sema3d cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3d
Entrez Gene ID	30250
Full Name	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms	sema2,semaZ2
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D Names semaphorin-3D sema Z2 semaphorin 3d semaphorin-2 semaphorin-Z2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131048.2	NP_571123.2	semaphorin-3D precursor

Pathways from BioSystems

Homologies

	SEMA3D	XP_001107897.1
Bos taurus (cattle)	SEMA3D	NP_001193025.1
Homo sapiens (human)	SEMA3D	NP_689967.2
Canis lupus familiaris (dog)	SEMA3D	XP_533138.2
Rattus norvegicus (Norway rat)	Sema3d	NP_001098103.1
Pan troglodytes (chimpanzee)	SEMA3D	XP_527805.2
Mus musculus (house mouse)	Sema3d	NP_083158.3
Gallus gallus (chicken)	SEMA3D	NP_990704.1
Danio rerio (zebrafish)	sema3d	NP_571123.1
Xenopus tropicalis (tropical clawed frog)	sema3d	XP_002935293.2

Gene Ontology
Bibliography

Related articles in PubMed

Cohesin mediates Esco2-dependent transcriptional regulation in a zebrafish regenerating fin model of Roberts Syndrome.
Banerji R, Skibbens RV, Iovine MK
Biology open6(12)1802-1813(2017 Dec)

Brorin is required for neurogenesis, gliogenesis, and commissural axon guidance in the zebrafish forebrain.
Miyake A, Mekata Y, Fujibayashi H, Nakanishi K, Konishi M, Itoh N
PloS one12(4)e0176036(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sema3d regulates collective endothelial cell migration in zebrafish through two separate mechanisms. Mesenchymal Sema3d guides outgrowth of the common cardinal vein via repulsion and signals through PlexinD1.
Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.

Hapln1a-ECM stabilizes the secreted growth factor Semaphorin3d (Sema3d), which has been independently shown to mediate Cx43 dependent phenotypes during regeneration.
Title: Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration.

Sema3d functions in a common molecular pathway with Cx43 in cell proliferation and joint formation
Title: Semaphorin3d mediates Cx43-dependent phenotypes during fin regeneration.

We show that Semaphorin3D (Sema3D), a classic repulsive molecule, promotes fasciculation by regulating L1 CAM levels and axon-axon interactions rather than by creating a repulsive surround.
Title: Semaphorin3D regulates axon axon interactions by modulating levels of L1 cell adhesion molecule.

Sema3D acts in concert with Nrp1a to guide neurons on the left side of the brain to innervate the target nucleus differently than those on the right side.
Title: Neuropilin asymmetry mediates a left-right difference in habenular connectivity.

The following sema3d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98346	NM_131048.2 Latest version!	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3d ( NM_131048.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571123.2 Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), mRNA.

Price & Availability↑

CloneID	ODa98346
Clone ID Related Accession (Same CDS sequence)	NM_131048.2
Accession Version	NM_131048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-3D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU855784.7, CU633862.5 and CU861648.4. On Feb 2, 2016 this sequence version replaced NM_131048.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124485.1, GDQH01020797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACTG CAGGGGAGCC GGACAGACGG AGACAGAGGA GACAGGTCAG GACGGGACGG 
TTTTCATGTG CTTGGTGGTC TACATCAGTG ATGCTCTTCT TCTCTCTGCC TGAGGGGAAC 
TGCATGAAGG AAAGTTTGCC AAGAGTTAAA CTCGGCTACA AAGATCTGAT ACATTCTCGA 
AGTGTAGTAC CATTTACGGG TTCCAGCGAA GGGCAGCATT TTCAGACAGT GTTATTGGAT 
GAGGAGAGAA GCCGATTACT GCTGGGAGCC AAAGACCACG TCTACCTACT GGACCCAGAC 
AACATCAATA AACACCCCAA AAAGTTGAGC TGGCCTGCTT CGAGAGACAG GGTGGAAATG 
TGTATACTGG CTGGTAAAAA CCCTCTTACT GAATGTGCAA ATTTCATACG AGTTTTGCAC 
AGCTATAACC GGACCCATGT TTACGCCTGT GGCACTGGAG CCTTCCATCC TACATGTGCC 
TTCTTAGAAA TTAAAGGTCA CAAAGAGGAC CGCTGGCTGC AGCTTCACAG CAACACTATG 
GAATCTGGCC GAATGAAATG TCCTTTTGAT CCTAATCAGC CTTTTGCTTC AGTGCTCACA 
GATCAGTATC TGTACGCTGG CACGGCGTCT GATTTCCTGG GCAAGGACAG CACGTTTACC 
CGCTCTCTCG GCCCACCTCC ACACCAGCAG TACATTCGCA CAGACATCTC AGAAGACTAC 
TGGATCAACG AGGGCAAGTT CATTTCTGCC CATCCCATCT CAGACACCTA CAATCCTGAC 
GATGACAAGA TTTACTTCTT TTTCCGTGAG GCTTCTCGTG ATGGGAGCAC TACAGACAAG 
AGCGTGCTGT CTCGTGTCGC CCGGATCTGC AGGAACGATG TTGGAGGTTT GAGAAGTCTT 
ACTAACAAGT GGACAACATT TCTCAAAGCG AGACTCGTGT GCTCCATTCC TGGACCAGAT 
GGAGTGGACA CACACTTTGA TGAGCTCCAG GACATCTTTC TTCTCCCTAG CAGAGATGAG 
AAAAACCCTA TGGTGTATGG AGTGTTCACC ACTACCAGTT CCATCTTTAA AGGATCAGCA 
GTGTGCGTCT ATACAATGGA AGACATCCGT GCAGCGTTTA ATGGACCGTA TGCTCATAAA 
GAGGGACCAG ATCATCGCTG GGTGGAATAT GAAGGCAGAA TTCCCTACCC ACGTCCAGGC 
ACTTGCCCCA GTCGGACATA TGATCCTCAT ATAAAAACAA CGAAAGACTT TCCAGATGAA 
GTCATCAGTT TTATACGATT GCACCCACTC ATGTACCAGT CTGTGCACCC AATGACAGGC 
CGACCAATTT TCACTCGTAT CAATACTGAG TACCGGCTTA CTCAGATCAT AGTGGACCGC 
GTGGCAGCTG AGGATGGACA GTATGCTGTT ATGTTCTTGG GCACAGATAT GGGATCCGTA 
TTGAAGGTAG TCAGCATCAC TCAAGAAAAC TGGTCCTCAG AAGAGATCAT CTTAGAGGAG 
CTTCAGGTGT TCAAGAATCC TTCACCCATC CTCAACATGG AAGTCTCTTC AAAGCAGCAG 
CAGCTGTTTG TCGGAGGAAG CGACGGGTTG GTGCAGGTGT CACTGCACAG GTGCCATATT 
TATGGACAGG GCTGTGCTGA ATGCTGTCTA GCAAGAGACC CCTACTGCGC TTGGGACGGC 
ACCCAGTGCT CCAGATACAT CCCAGCTTCC AAAAGAAGGG CCAGAAGGCA GGACATCAAA 
CATGGAGACC CATCCAGCCA TTGTTGGGAC ACAGAGGATG TGTTAGGGAG GAATGTCGAG 
GAGAAGGTGT TGTACGGAGT GGAGAGTAAC TCCTCTTTTC TTGAGTGCGT TTCGAAATCC 
CAGCAGGCCT TGATCCGCTG GTTTGTACTG AAGCCAGGAG TGGACCACCG TCAAGAGATT 
AAACCAGACG AGCGTGTGCT GATAACCGAT CGAGGTTTGC TGATTCGCTG GCTCCAACGT 
GGCGATGCGG GGTCCTACTT TTGCACGTCT CAGGAGCACA GATTCACCCG AACCCTCCTC 
CACGTCTCTC TCCACATTCT GGACCGCGGA CAAATCAATG CACATCAACC TGCTATACGG 
GAATCATCTG AAAACCCTGC CGTGACTGAA CCCCGCCAGC GCTATAAGGA TTATTTGCGC 
ATGTTGAGCG GCCCTGCACG ATCTCTGGAT GAGTACTGTG AAACCATGTG GCATAGGGAG 
AAGAAGCAGA AGCAGAAGGG CAAATGGAAA CACGTTCAAG AGCTCCGCAA AAGCAGAAAC 
CGACGCCACC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571123.2
CDS	269..2563
Translation	MKTAGEPDRRRQRRQVRTGRFSCAWWSTSVMLFFSLPEGNCMKESLPRVKLGYKDLIHSRSVVPFTGSSEGQHFQTVLLDEERSRLLLGAKDHVYLLDPDNINKHPKKLSWPASRDRVEMCILAGKNPLTECANFIRVLHSYNRTHVYACGTGAFHPTCAFLEIKGHKEDRWLQLHSNTMESGRMKCPFDPNQPFASVLTDQYLYAGTASDFLGKDSTFTRSLGPPPHQQYIRTDISEDYWINEGKFISAHPISDTYNPDDDKIYFFFREASRDGSTTDKSVLSRVARICRNDVGGLRSLTNKWTTFLKARLVCSIPGPDGVDTHFDELQDIFLLPSRDEKNPMVYGVFTTTSSIFKGSAVCVYTMEDIRAAFNGPYAHKEGPDHRWVEYEGRIPYPRPGTCPSRTYDPHIKTTKDFPDEVISFIRLHPLMYQSVHPMTGRPIFTRINTEYRLTQIIVDRVAAEDGQYAVMFLGTDMGSVLKVVSITQENWSSEEIILEELQVFKNPSPILNMEVSSKQQQLFVGGSDGLVQVSLHRCHIYGQGCAECCLARDPYCAWDGTQCSRYIPASKRRARRQDIKHGDPSSHCWDTEDVLGRNVEEKVLYGVESNSSFLECVSKSQQALIRWFVLKPGVDHRQEIKPDERVLITDRGLLIRWLQRGDAGSYFCTSQEHRFTRTLLHVSLHILDRGQINAHQPAIRESSENPAVTEPRQRYKDYLRMLSGPARSLDEYCETMWHREKKQKQKGKWKHVQELRKSRNRRHH

Target ORF information:

RefSeq Version	NM_131048.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (sema3d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131048.2

ORF Insert Sequence:

ATGAAGACTG CAGGGGAGCC GGACAGACGG AGACAGAGGA GACAGGTCAG GACGGGACGG 
TTTTCATGTG CTTGGTGGTC TACATCAGTG ATGCTCTTCT TCTCTCTGCC TGAGGGGAAC 
TGCATGAAGG AAAGTTTGCC AAGAGTTAAA CTCGGCTACA AAGATCTGAT ACATTCTCGA 
AGTGTAGTAC CATTTACGGG TTCCAGCGAA GGGCAGCATT TTCAGACAGT GTTATTGGAT 
GAGGAGAGAA GCCGATTACT GCTGGGAGCC AAAGACCACG TCTACCTACT GGACCCAGAC 
AACATCAATA AACACCCCAA AAAGTTGAGC TGGCCTGCTT CGAGAGACAG GGTGGAAATG 
TGTATACTGG CTGGTAAAAA CCCTCTTACT GAATGTGCAA ATTTCATACG AGTTTTGCAC 
AGCTATAACC GGACCCATGT TTACGCCTGT GGCACTGGAG CCTTCCATCC TACATGTGCC 
TTCTTAGAAA TTAAAGGTCA CAAAGAGGAC CGCTGGCTGC AGCTTCACAG CAACACTATG 
GAATCTGGCC GAATGAAATG TCCTTTTGAT CCTAATCAGC CTTTTGCTTC AGTGCTCACA 
GATCAGTATC TGTACGCTGG CACGGCGTCT GATTTCCTGG GCAAGGACAG CACGTTTACC 
CGCTCTCTCG GCCCACCTCC ACACCAGCAG TACATTCGCA CAGACATCTC AGAAGACTAC 
TGGATCAACG AGGGCAAGTT CATTTCTGCC CATCCCATCT CAGACACCTA CAATCCTGAC 
GATGACAAGA TTTACTTCTT TTTCCGTGAG GCTTCTCGTG ATGGGAGCAC TACAGACAAG 
AGCGTGCTGT CTCGTGTCGC CCGGATCTGC AGGAACGATG TTGGAGGTTT GAGAAGTCTT 
ACTAACAAGT GGACAACATT TCTCAAAGCG AGACTCGTGT GCTCCATTCC TGGACCAGAT 
GGAGTGGACA CACACTTTGA TGAGCTCCAG GACATCTTTC TTCTCCCTAG CAGAGATGAG 
AAAAACCCTA TGGTGTATGG AGTGTTCACC ACTACCAGTT CCATCTTTAA AGGATCAGCA 
GTGTGCGTCT ATACAATGGA AGACATCCGT GCAGCGTTTA ATGGACCGTA TGCTCATAAA 
GAGGGACCAG ATCATCGCTG GGTGGAATAT GAAGGCAGAA TTCCCTACCC ACGTCCAGGC 
ACTTGCCCCA GTCGGACATA TGATCCTCAT ATAAAAACAA CGAAAGACTT TCCAGATGAA 
GTCATCAGTT TTATACGATT GCACCCACTC ATGTACCAGT CTGTGCACCC AATGACAGGC 
CGACCAATTT TCACTCGTAT CAATACTGAG TACCGGCTTA CTCAGATCAT AGTGGACCGC 
GTGGCAGCTG AGGATGGACA GTATGCTGTT ATGTTCTTGG GCACAGATAT GGGATCCGTA 
TTGAAGGTAG TCAGCATCAC TCAAGAAAAC TGGTCCTCAG AAGAGATCAT CTTAGAGGAG 
CTTCAGGTGT TCAAGAATCC TTCACCCATC CTCAACATGG AAGTCTCTTC AAAGCAGCAG 
CAGCTGTTTG TCGGAGGAAG CGACGGGTTG GTGCAGGTGT CACTGCACAG GTGCCATATT 
TATGGACAGG GCTGTGCTGA ATGCTGTCTA GCAAGAGACC CCTACTGCGC TTGGGACGGC 
ACCCAGTGCT CCAGATACAT CCCAGCTTCC AAAAGAAGGG CCAGAAGGCA GGACATCAAA 
CATGGAGACC CATCCAGCCA TTGTTGGGAC ACAGAGGATG TGTTAGGGAG GAATGTCGAG 
GAGAAGGTGT TGTACGGAGT GGAGAGTAAC TCCTCTTTTC TTGAGTGCGT TTCGAAATCC 
CAGCAGGCCT TGATCCGCTG GTTTGTACTG AAGCCAGGAG TGGACCACCG TCAAGAGATT 
AAACCAGACG AGCGTGTGCT GATAACCGAT CGAGGTTTGC TGATTCGCTG GCTCCAACGT 
GGCGATGCGG GGTCCTACTT TTGCACGTCT CAGGAGCACA GATTCACCCG AACCCTCCTC 
CACGTCTCTC TCCACATTCT GGACCGCGGA CAAATCAATG CACATCAACC TGCTATACGG 
GAATCATCTG AAAACCCTGC CGTGACTGAA CCCCGCCAGC GCTATAAGGA TTATTTGCGC 
ATGTTGAGCG GCCCTGCACG ATCTCTGGAT GAGTACTGTG AAACCATGTG GCATAGGGAG 
AAGAAGCAGA AGCAGAAGGG CAAATGGAAA CACGTTCAAG AGCTCCGCAA AAGCAGAAAC 
CGACGCCACC ACTAG