mest cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mest
Entrez Gene ID	30242
Full Name	mesoderm specific transcript
Synonyms	Zpeg1,peg1,peg1/mest
General protein information	Preferred Names mesoderm specific transcript Names mesoderm-specific transcript homolog protein alpha/beta hydroxylase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328532.1	NP_001315461.1	mesoderm-specific transcript homolog protein isoform 2
NM_131043.2	NP_571118.2	mesoderm-specific transcript homolog protein isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MEST	XP_519382.3
Homo sapiens (human)	MEST	NP_002393.2
Bos taurus (cattle)	MEST	NP_001076837.1
Mus musculus (house mouse)	Mest	NP_032616.1
Rattus norvegicus (Norway rat)	Mest	NP_001009617.1
Gallus gallus (chicken)	MEST	XP_425258.3
	MEST	XP_001097736.1
Canis lupus familiaris (dog)	MEST	XP_848679.1
Xenopus tropicalis (tropical clawed frog)	mest	NP_001005789.1
Danio rerio (zebrafish)	mest	NP_571118.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/MEST.
Hahn Y, Yang SK, Chung JH
Biochimica et biophysica acta1731(2)125-32(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we report identification of zebrafish (Danio rerio) gene mest, an ortholog of mammalian PEG1/MEST.
Title: Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/MEST.

The following mest gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mest cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa09315	NM_001328532.1 Latest version!	Danio rerio mesoderm specific transcript (mest), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa53403	NM_131043.2 Latest version!	Danio rerio mesoderm specific transcript (mest), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mest ( NM_001328532.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315461.1 Danio rerio mesoderm specific transcript (mest), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa09315
Clone ID Related Accession (Same CDS sequence)	NM_001328532.1
Accession Version	NM_001328532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mesoderm-specific transcript homolog protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR749763.6. On Jun 17, 2016 this sequence version replaced XM_005164686.2. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC059471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGCG ACTCACCTAA TGACAGTGAG TGTGGGTCAG CTCGTCGTGT GAGCAGAGAA 
TGGTGGCTTC ACGTGGGTTT GTTGTGTGTT CCTCTGCTCG CCGTGTATCT GCACATCCCT 
CCGCCACAGC TGTCACCTGC GCTTAACAGC TGGCGCACTT CCGGTCACTT CTTCACATTC 
AGAGGCAATG ACATCTTCTA TAAAGAGTCA GTTGGAGTTG TGGGAAGCTC TGATGTCCTT 
GTGTTGCTTC ATGGCTTTCC TACGTCAAGC TATGACTGGT ATAAGATCTG GGATTCTCTG 
ACGCAGCGCT TCAATAGAGT CATCGCCCTT GACTTCCTGG GCTTCGGTTT TTCTGACAAA 
CCGAGGCCGC ACCGGTACTC CATCTTCGAG CAGGCCAGTG TTGTGGAAGC TCTAGTTGCC 
CATTTGGGTT TGTCAGAGCA GAGGATCAAC ATCCTATCAC ACGACTACGG AGACACTGTT 
GCTCTCGAGT TACTGTACAG GAGTGACCAC AACAGAAGCG GACACATCAT TGTAAACAGC 
CTCTGCCTAT CAAATGGAGG TATATTCCCA GAGACTCATC ATCCAAGATT CCTTCAGAAA 
GTGCTGAAGG ACTCCGGCTT TATTTCACCT GTACTCACTC GTCTGATGAA CTTCCAGCTT 
TTCTCCAGAG GAATTAAGGA AGTGTTTGGA CCGTACACCC AGCCAACAGA AGCAGAAGTC 
TGGGACATGT GGACAGGCAT TCGCTTTAAT GATGGAAATC TTGTAATGGA CAGTCTTTTG 
CAATACATTA ACCAGAGGCT GAAACACAGA GAACGATGGG TGGGCGCTCT AACTTCGACT 
TTAACACCCC TGCACATGAT TTATGGACCC CTAGATCCAG TTAATCCACA TCCGCAGTTT 
CTGCAGCTTT ACCAGAAACT AGTGCAGAGA TCCACAGTAT CTGTTCTAGA TGAGCACGTG 
AGTCATTATC CACAGCTGGA GGATCCCACA GGATTCTTTA ATGCCTATCT CAGCTTTATC 
AACTCATTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315461.1
CDS	167..1198
Translation	MSGDSPNDSECGSARRVSREWWLHVGLLCVPLLAVYLHIPPPQLSPALNSWRTSGHFFTFRGNDIFYKESVGVVGSSDVLVLLHGFPTSSYDWYKIWDSLTQRFNRVIALDFLGFGFSDKPRPHRYSIFEQASVVEALVAHLGLSEQRINILSHDYGDTVALELLYRSDHNRSGHIIVNSLCLSNGGIFPETHHPRFLQKVLKDSGFISPVLTRLMNFQLFSRGIKEVFGPYTQPTEAEVWDMWTGIRFNDGNLVMDSLLQYINQRLKHRERWVGALTSTLTPLHMIYGPLDPVNPHPQFLQLYQKLVQRSTVSVLDEHVSHYPQLEDPTGFFNAYLSFINSF

Target ORF information:

RefSeq Version	NM_001328532.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesoderm specific transcript (mest), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328532.1

ORF Insert Sequence:

ATGTCAGGCG ACTCACCTAA TGACAGTGAG TGTGGGTCAG CTCGTCGTGT GAGCAGAGAA 
TGGTGGCTTC ACGTGGGTTT GTTGTGTGTT CCTCTGCTCG CCGTGTATCT GCACATCCCT 
CCGCCACAGC TGTCACCTGC GCTTAACAGC TGGCGCACTT CCGGTCACTT CTTCACATTC 
AGAGGCAATG ACATCTTCTA TAAAGAGTCA GTTGGAGTTG TGGGAAGCTC TGATGTCCTT 
GTGTTGCTTC ATGGCTTTCC TACGTCAAGC TATGACTGGT ATAAGATCTG GGATTCTCTG 
ACGCAGCGCT TCAATAGAGT CATCGCCCTT GACTTCCTGG GCTTCGGTTT TTCTGACAAA 
CCGAGGCCGC ACCGGTACTC CATCTTCGAG CAGGCCAGTG TTGTGGAAGC TCTAGTTGCC 
CATTTGGGTT TGTCAGAGCA GAGGATCAAC ATCCTATCAC ACGACTACGG AGACACTGTT 
GCTCTCGAGT TACTGTACAG GAGTGACCAC AACAGAAGCG GACACATCAT TGTAAACAGC 
CTCTGCCTAT CAAATGGAGG TATATTCCCA GAGACTCATC ATCCAAGATT CCTTCAGAAA 
GTGCTGAAGG ACTCCGGCTT TATTTCACCT GTACTCACTC GTCTGATGAA CTTCCAGCTT 
TTCTCCAGAG GAATTAAGGA AGTGTTTGGA CCGTACACCC AGCCAACAGA AGCAGAAGTC 
TGGGACATGT GGACAGGCAT TCGCTTTAAT GATGGAAATC TTGTAATGGA CAGTCTTTTG 
CAATACATTA ACCAGAGGCT GAAACACAGA GAACGATGGG TGGGCGCTCT AACTTCGACT 
TTAACACCCC TGCACATGAT TTATGGACCC CTAGATCCAG TTAATCCACA TCCGCAGTTT 
CTGCAGCTTT ACCAGAAACT AGTGCAGAGA TCCACAGTAT CTGTTCTAGA TGAGCACGTG 
AGTCATTATC CACAGCTGGA GGATCCCACA GGATTCTTTA ATGCCTATCT CAGCTTTATC 
AACTCATTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mest ( NM_131043.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571118.2 Danio rerio mesoderm specific transcript (mest), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa53403
Clone ID Related Accession (Same CDS sequence)	NM_131043.2
Accession Version	NM_131043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mesoderm-specific transcript homolog protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR749763.6. On Jun 17, 2016 this sequence version replaced NM_131043.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF194334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGCG ACTCACCTCA GAATGACAGT GAGTGTGGGT CAGCTCGTCG TGTGAGCAGA 
GAATGGTGGC TTCACGTGGG TTTGTTGTGT GTTCCTCTGC TCGCCGTGTA TCTGCACATC 
CCTCCGCCAC AGCTGTCACC TGCGCTTAAC AGCTGGCGCA CTTCCGGTCA CTTCTTCACA 
TTCAGAGGCA ATGACATCTT CTATAAAGAG TCAGTTGGAG TTGTGGGAAG CTCTGATGTC 
CTTGTGTTGC TTCATGGCTT TCCTACGTCA AGCTATGACT GGTATAAGAT CTGGGATTCT 
CTGACGCAGC GCTTCAATAG AGTCATCGCC CTTGACTTCC TGGGCTTCGG TTTTTCTGAC 
AAACCGAGGC CGCACCGGTA CTCCATCTTC GAGCAGGCCA GTGTTGTGGA AGCTCTAGTT 
GCCCATTTGG GTTTGTCAGA GCAGAGGATC AACATCCTAT CACACGACTA CGGAGACACT 
GTTGCTCTCG AGTTACTGTA CAGGAGTGAC CACAACAGAA GCGGACACAT CATTGTAAAC 
AGCCTCTGCC TATCAAATGG AGGTATATTC CCAGAGACTC ATCATCCAAG ATTCCTTCAG 
AAAGTGCTGA AGGACTCCGG CTTTATTTCA CCTGTACTCA CTCGTCTGAT GAACTTCCAG 
CTTTTCTCCA GAGGAATTAA GGAAGTGTTT GGACCGTACA CCCAGCCAAC AGAAGCAGAA 
GTCTGGGACA TGTGGACAGG CATTCGCTTT AATGATGGAA ATCTTGTAAT GGACAGTCTT 
TTGCAATACA TTAACCAGAG GCTGAAACAC AGAGAACGAT GGGTGGGCGC TCTAACTTCG 
ACTTTAACAC CCCTGCACAT GATTTATGGA CCCCTAGATC CAGTTAATCC ACATCCGCAG 
TTTCTGCAGC TTTACCAGAA ACTAGTGCAG AGATCCACAG TATCTGTTCT AGATGAGCAC 
GTGAGTCATT ATCCACAGCT GGAGGATCCC ACAGGATTCT TTAATGCCTA TCTCAGCTTT 
ATCAACTCAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571118.2
CDS	167..1201
Translation	MSGDSPQNDSECGSARRVSREWWLHVGLLCVPLLAVYLHIPPPQLSPALNSWRTSGHFFTFRGNDIFYKESVGVVGSSDVLVLLHGFPTSSYDWYKIWDSLTQRFNRVIALDFLGFGFSDKPRPHRYSIFEQASVVEALVAHLGLSEQRINILSHDYGDTVALELLYRSDHNRSGHIIVNSLCLSNGGIFPETHHPRFLQKVLKDSGFISPVLTRLMNFQLFSRGIKEVFGPYTQPTEAEVWDMWTGIRFNDGNLVMDSLLQYINQRLKHRERWVGALTSTLTPLHMIYGPLDPVNPHPQFLQLYQKLVQRSTVSVLDEHVSHYPQLEDPTGFFNAYLSFINSF

Target ORF information:

RefSeq Version	NM_131043.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesoderm specific transcript (mest), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131043.2

ORF Insert Sequence:

ATGTCAGGCG ACTCACCTCA GAATGACAGT GAGTGTGGGT CAGCTCGTCG TGTGAGCAGA 
GAATGGTGGC TTCACGTGGG TTTGTTGTGT GTTCCTCTGC TCGCCGTGTA TCTGCACATC 
CCTCCGCCAC AGCTGTCACC TGCGCTTAAC AGCTGGCGCA CTTCCGGTCA CTTCTTCACA 
TTCAGAGGCA ATGACATCTT CTATAAAGAG TCAGTTGGAG TTGTGGGAAG CTCTGATGTC 
CTTGTGTTGC TTCATGGCTT TCCTACGTCA AGCTATGACT GGTATAAGAT CTGGGATTCT 
CTGACGCAGC GCTTCAATAG AGTCATCGCC CTTGACTTCC TGGGCTTCGG TTTTTCTGAC 
AAACCGAGGC CGCACCGGTA CTCCATCTTC GAGCAGGCCA GTGTTGTGGA AGCTCTAGTT 
GCCCATTTGG GTTTGTCAGA GCAGAGGATC AACATCCTAT CACACGACTA CGGAGACACT 
GTTGCTCTCG AGTTACTGTA CAGGAGTGAC CACAACAGAA GCGGACACAT CATTGTAAAC 
AGCCTCTGCC TATCAAATGG AGGTATATTC CCAGAGACTC ATCATCCAAG ATTCCTTCAG 
AAAGTGCTGA AGGACTCCGG CTTTATTTCA CCTGTACTCA CTCGTCTGAT GAACTTCCAG 
CTTTTCTCCA GAGGAATTAA GGAAGTGTTT GGACCGTACA CCCAGCCAAC AGAAGCAGAA 
GTCTGGGACA TGTGGACAGG CATTCGCTTT AATGATGGAA ATCTTGTAAT GGACAGTCTT 
TTGCAATACA TTAACCAGAG GCTGAAACAC AGAGAACGAT GGGTGGGCGC TCTAACTTCG 
ACTTTAACAC CCCTGCACAT GATTTATGGA CCCCTAGATC CAGTTAATCC ACATCCGCAG 
TTTCTGCAGC TTTACCAGAA ACTAGTGCAG AGATCCACAG TATCTGTTCT AGATGAGCAC 
GTGAGTCATT ATCCACAGCT GGAGGATCCC ACAGGATTCT TTAATGCCTA TCTCAGCTTT 
ATCAACTCAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/MEST.
Biochimica et biophysica acta1731(2)125-32(2005 Nov)
Hahn Y,Yang SK,Chung JH