gbx2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gbx2
Entrez Gene ID	245948
Full Name	gastrulation brain homeobox 2
Synonyms	fd07f12,gbx-2,wu:fd07f12
General protein information	Preferred Names gastrulation brain homeobox 2 Names homeobox protein GBX-2 gastrulation brain homeo box 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_152964.1	NP_694496.1	homeobox protein GBX-2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	gbx2	NP_694496.1
Xenopus tropicalis (tropical clawed frog)	gbx2	XP_002932046.1
Homo sapiens (human)	GBX2	NP_001476.2
Mus musculus (house mouse)	Gbx2	NP_034392.1
Rattus norvegicus (Norway rat)	Gbx2	NP_446160.1
Xenopus tropicalis (tropical clawed frog)	gbx2.2	NP_001011472.1
Pan troglodytes (chimpanzee)	GBX2	XP_526072.2
	GBX2	XP_001082998.1
Canis lupus familiaris (dog)	GBX2	XP_543300.1
Bos taurus (cattle)	GBX2	NP_001179074.1
Gallus gallus (chicken)	GBX2	NP_990399.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of gastrulation brain homeobox 2 (gbx2) in the development of the ventral telencephalon in zebrafish embryos.
Wang Z, Nakayama Y, Tsuda S, Yamasu K
Differentiation; research in biological diversity9928-40(2017 Dec)

Transcriptional profiling of human neural precursors post alcohol exposure reveals impaired neurogenesis via dysregulation of ERK signaling and miR-145.
Louis LK, Gopurappilly R, Surendran H, Dutta S, Pal R
Journal of neurochemistry(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gbx2 not only functions to position the midbrain-hindbrain boundary but also regulates neurogenesis in the anterior hindbrain.
Title: Comprehensive analysis of target genes in zebrafish embryos reveals gbx2 involvement in neurogenesis.

in the zebrafish gbx1 and gbx2 are required redundantly for morphogenesis of the cerebellar primordium and subsequent cerebellar differentiation, but that this requirement is alleviated by knocking down Otx
Title: Cerebellar development in the absence of Gbx function in zebrafish.

Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner.
Title: Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner.

gbx2/Gbx2 has an evolutionarily conserved role in anterior hindbrain development.
Title: Evolutionarily conserved function of Gbx2 in anterior hindbrain development.

gbx2 expression during the somitogenesis stage in zebrafish is regulated by a complex mechanism involving Pax2 as well as activators and suppressors in the regions flanking the gene.
Title: Three enhancer regions regulate gbx2 gene expression in the isthmic region during zebrafish development.

The following gbx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02877	NM_152964.1 Latest version!	Danio rerio gastrulation brain homeobox 2 (gbx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gbx2 ( NM_152964.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694496.1 Danio rerio gastrulation brain homeobox 2 (gbx2), mRNA.

Price & Availability↑

CloneID	ODa02877
Clone ID Related Accession (Same CDS sequence)	NM_152964.1
Accession Version	NM_152964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Danio rerio(zebrafish)
Product	homeobox protein GBX-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB075028.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB075028.1, AF422807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGCAG CTTTCAGCAC ACCGTTCATG ATGATGCAGC GTCCGGTGGG AAGCACCACT 
GCATTCAGCA TTGACTCGCT CATCGGCGGT CCGCCGCAGC CCAGTCCGGG ACATTTCGTG 
TACACAGGTT ATCCCATGTT CATGCCATAC CGGTCAGTGG TGCTTCCTCC GCCGCCTCCT 
CCACCCCCTC CAACACTCCC CCAAAGCGCT CTCCCTACGA CCCATCCGCA CCACCCGATC 
CCGGGCTTAC CCAGCAGCTT CTGCTCCAGC CTGGCGCAGG GCATGGCACT CACATCCACG 
CTAATGGCCA CGTTACCCGG CGGCTTCTCC TCCTCGCCGT CCCAACAGCA CCAAGACGCG 
GCGAGGAAGC TCGGCTCTCA GTCTATTCAC GCCATGTTCG ACAAATCTCA GGATATTCGT 
TTGGATGGAG AGGATGGGAA AACGTTTGCG ACGAAAGATT CGACGAGCAT TCCGTCCTTC 
CACGATTCGC AGTCCGTACA CACCTCTACA GTGCGAGGTC ACAGCAAAGA CGACTCGAAG 
GAGGATGATT GTCACAGGAA AGACGAGAGC TTCTCCATGG ACAGTGATTT AGATTACAGC 
TCCGATGATA ACGGGCCCGG GAACGCCATG TGTCAGAAAG AAGACGGAGA CGGCAGCGGA 
GGTCTGGACG ACGGCGTTCA CGGTGGGAAT GGGGCCGGGA ACACCACATC CACTGGGAAA 
AATCGGAGAA GGAGGACCGC TTTTACGAGC GAGCAACTGT TGGAACTGGA GAAGGAGTTT 
CACTGTAAGA AATATCTGTC CCTCACAGAA CGCTCGCAAA TCGCGCACGC CTTAAAGCTC 
AGCGAGGTGC AGGTCAAGAT CTGGTTTCAG AACCGGAGAG CCAAGTGGAA ACGGGTCAAA 
GCGGGCAACG TCAACAGCAA AACCGGAGAG CCATCCAGAA ACCCCAAAAT TGTGGTGCCC 
ATTCCGGTGC ATGTTAGTCG GTTTGCGATA CGCAGCCAAC ACCAACAGTT AGAACAGGCC 
CGACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694496.1
CDS	413..1441
Translation	MSAAFSTPFMMMQRPVGSTTAFSIDSLIGGPPQPSPGHFVYTGYPMFMPYRSVVLPPPPPPPPPTLPQSALPTTHPHHPIPGLPSSFCSSLAQGMALTSTLMATLPGGFSSSPSQQHQDAARKLGSQSIHAMFDKSQDIRLDGEDGKTFATKDSTSIPSFHDSQSVHTSTVRGHSKDDSKEDDCHRKDESFSMDSDLDYSSDDNGPGNAMCQKEDGDGSGGLDDGVHGGNGAGNTTSTGKNRRRRTAFTSEQLLELEKEFHCKKYLSLTERSQIAHALKLSEVQVKIWFQNRRAKWKRVKAGNVNSKTGEPSRNPKIVVPIPVHVSRFAIRSQHQQLEQARP

Target ORF information:

RefSeq Version	NM_152964.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gastrulation brain homeobox 2 (gbx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152964.1

ORF Insert Sequence:

ATGAGTGCAG CTTTCAGCAC ACCGTTCATG ATGATGCAGC GTCCGGTGGG AAGCACCACT 
GCATTCAGCA TTGACTCGCT CATCGGCGGT CCGCCGCAGC CCAGTCCGGG ACATTTCGTG 
TACACAGGTT ATCCCATGTT CATGCCATAC CGGTCAGTGG TGCTTCCTCC GCCGCCTCCT 
CCACCCCCTC CAACACTCCC CCAAAGCGCT CTCCCTACGA CCCATCCGCA CCACCCGATC 
CCGGGCTTAC CCAGCAGCTT CTGCTCCAGC CTGGCGCAGG GCATGGCACT CACATCCACG 
CTAATGGCCA CGTTACCCGG CGGCTTCTCC TCCTCGCCGT CCCAACAGCA CCAAGACGCG 
GCGAGGAAGC TCGGCTCTCA GTCTATTCAC GCCATGTTCG ACAAATCTCA GGATATTCGT 
TTGGATGGAG AGGATGGGAA AACGTTTGCG ACGAAAGATT CGACGAGCAT TCCGTCCTTC 
CACGATTCGC AGTCCGTACA CACCTCTACA GTGCGAGGTC ACAGCAAAGA CGACTCGAAG 
GAGGATGATT GTCACAGGAA AGACGAGAGC TTCTCCATGG ACAGTGATTT AGATTACAGC 
TCCGATGATA ACGGGCCCGG GAACGCCATG TGTCAGAAAG AAGACGGAGA CGGCAGCGGA 
GGTCTGGACG ACGGCGTTCA CGGTGGGAAT GGGGCCGGGA ACACCACATC CACTGGGAAA 
AATCGGAGAA GGAGGACCGC TTTTACGAGC GAGCAACTGT TGGAACTGGA GAAGGAGTTT 
CACTGTAAGA AATATCTGTC CCTCACAGAA CGCTCGCAAA TCGCGCACGC CTTAAAGCTC 
AGCGAGGTGC AGGTCAAGAT CTGGTTTCAG AACCGGAGAG CCAAGTGGAA ACGGGTCAAA 
GCGGGCAACG TCAACAGCAA AACCGGAGAG CCATCCAGAA ACCCCAAAAT TGTGGTGCCC 
ATTCCGGTGC ATGTTAGTCG GTTTGCGATA CGCAGCCAAC ACCAACAGTT AGAACAGGCC 
CGACCTTGA