gpc4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpc4
Entrez Gene ID	118437
Full Name	glypican 4
Synonyms	chunp6920,gpc4,,gpc4/6,gpc6,kny,knypek,wu:fc47a08,wu:fe05f10,zgc:194854
General protein information	Preferred Names glypican 4 Names glypican-4 fc47a08 fe05f10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131860.2	NP_571935.2	glypican-4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpc4	NP_001014130.1
Bos taurus (cattle)	GPC4	NP_001192713.1
Mus musculus (house mouse)	Gpc4	NP_032176.2
Gallus gallus (chicken)	GPC4	XP_420222.3
Danio rerio (zebrafish)	gpc4	NP_571935.2
Xenopus tropicalis (tropical clawed frog)	gpc4	XP_002932634.1
Canis lupus familiaris (dog)	GPC4	XP_549265.2
	GPC4	XP_001097252.1
Drosophila melanogaster (fruit fly)	dlp	NP_996075.2
Caenorhabditis elegans (roundworm)	gpn-1	NP_510582.1
Homo sapiens (human)	GPC4	NP_001439.2
Pan troglodytes (chimpanzee)	GPC4	XP_529161.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003559	XP_313306.5

Gene Ontology
Bibliography

Related articles in PubMed

Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification.
Ling IT, Rochard L, Liao EC
Developmental biology421(2)219-232(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification.
Title: Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification.

A mutation in glypican4 leads to severe lateral line primordium migration defects. Glypican4 regulates the feedback loop between Wnt/beta-catenin/Fgf signaling in the primordium redundantly with other HSPGs. A mutation in glypican4 affects primordium migration non cell-autonomously
Title: Glypican4 modulates lateral line collective cell migration non cell-autonomously.

the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4.
Title: Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis.

novel mechanism for Wnt5b and Gpc4 regulation of chondrocyte behavior that is independent of the core Wnt/PCP molecules and differs from their collaborative action of controlling cell movements during gastrulation
Title: A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis.

glypican4 mutants do not have decreased proteolysis of ECM substrates, but instead have increased cell surface cadherin protein expression and increased intercellular adhesion.
Title: Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation.

The following gpc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38765	NM_131860.2 Latest version!	Danio rerio glypican 4 (gpc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpc4 ( NM_131860.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571935.2 Danio rerio glypican 4 (gpc4), mRNA.

Price & Availability↑

CloneID	ODa38765
Clone ID Related Accession (Same CDS sequence)	NM_131860.2
Accession Version	NM_131860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	glypican-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163403.1. On Apr 15, 2009 this sequence version replaced NM_131860.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC163403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGATGA TCGTTGTGTT TACAGTATGT ATGTCTGTGG TGGTTTTGGC GTCGGCTCAG 
GCTGATCAGA AGTCAAAGAA CTGCAATGAA GTCAGAACTG CCTACAGTTC CAAAGGTTTC 
AACGTCAACG ATGTTCCCAA CAAAGGAGTG CAGGGAGCCC ATCTGAAGGT GTGTCCTCAA 
GGTTTCTCCT GCTGTACTCT GGAGATGGAG GAGAAGTTGA GTCAGCTGAG CCGCGTAGAC 
CTGAAAGTTC CCGTTCACCA GCTCAGCTCC AACCTGCAGA GCACCTTCAC CCAGAGACAC 
CGTCACTTTG ACCAGTTCTT CAGAGAGCTG TTGGACAATG CAGAGAAGTC TCTCAATCAC 
ATGTTTGTGC GGACGTACGG CTTGATGTAC GTCAAGAACA AAGAGCTCTT TGAGGGTTTC 
TTCAGTGATC TGCGCCGCTA CTACAGCCAC GGCAGCAGTG AGGTCAATCT GGACGACATG 
CTGGCCGAGT TCTGGTCTGA ACTGCTGGAG CGCATGTTTC GACTGGTCAA CGTCCAGTAC 
GAGTTCAGCG ACTCCTACAT GGAGTGTGTC AGCAGGCACA CGGACCAGCT CAAACCCTTC 
GGAGACGTTC CACGCAAGCT CCGTCTGCAG CTGACCCGCT CCTTCATTGC TGTTCGCGCC 
TTCACTCGTG GCCTCACACT CATGCCTGAT GTGGTCCGCA AAGTCTCAAC GGTGAGCGCC 
TCACCGAGCT GTGTGCGCGC ATCGATGAAG ATGTTATACT GCCCGTACTG TAGCGGTCAG 
GTGGCGCTGA AGCCCTGCAA GAACTACTGT CTGAATGTCA TGAGGGGCTG TCTGGCCAAT 
CAGGCCGATC TGGACACAGA GTGGAATAAC TTCCTTGACT CCATGTTAGG TCTCGCTGAG 
AGGCTGGAGG GACCCTTTAA CTTCGAGTCG GTCGTGGACC CCATCGATGT GAAGATTTCA 
GATGCTATCA TGAACATGCA GGAGAACAGC ATGCAGGTCT CACAGAAGGT GTTTCAGGGT 
TGTGGGCAGC CCAAACTCAG CATGGGCTTC CGCAGCCGGA GGTCTGCTAA AGACTCGGGC 
TTCCCTGGCC GTTTTAGACC GTACAGCCCT GAGGCCAGAC CCACCACTGC TGCTGGCACC 
ACCCTGGACC GACTGCTGAC GGATGTCAAG AAGAAACTGA AGCATGCCAA GAAGTTCTGG 
TCCACCCTGC CGGACACAGT GTGTGTTGGA GAAAGGATCG CCCCCAGTGA TGATTGCTGG 
AATGGTACAG CTAAAAGCAG GTACGAATCT GTGGTTATGA GCAGCGGTTT AGCCAATCAG 
GTGTCTAATC CAGACGTGGA GGTTGACATC ACCAAACCAG ACATGGTGAT CCGGCGGCAG 
ATCGCAGTCC TGAAGGAAAT GACCAGCTGG CTGAAAGCTG CTTACACCGG CAACGACATC 
TCATTTACCT CCGATGATGC CGGCAGCGGA GAGGAAAGCG GAAGCGGCTG CGACTCTCCC 
TCTTGCGAAG GCGACGGAGA TATTTACTTC TCCACCCCGG CACCTGGTAA ACCTCGCATC 
CTCAAGACTC CGGAGGTCGA ACAGGCCTCC AGCGGCACAC GGTTGGCACC CTGCAGCCTG 
GCACTGGCCC TGGCCTCCCT TCTGCTCACC CTGCTTACTC TCCAAACAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571935.2
CDS	90..1763
Translation	MKMIVVFTVCMSVVVLASAQADQKSKNCNEVRTAYSSKGFNVNDVPNKGVQGAHLKVCPQGFSCCTLEMEEKLSQLSRVDLKVPVHQLSSNLQSTFTQRHRHFDQFFRELLDNAEKSLNHMFVRTYGLMYVKNKELFEGFFSDLRRYYSHGSSEVNLDDMLAEFWSELLERMFRLVNVQYEFSDSYMECVSRHTDQLKPFGDVPRKLRLQLTRSFIAVRAFTRGLTLMPDVVRKVSTVSASPSCVRASMKMLYCPYCSGQVALKPCKNYCLNVMRGCLANQADLDTEWNNFLDSMLGLAERLEGPFNFESVVDPIDVKISDAIMNMQENSMQVSQKVFQGCGQPKLSMGFRSRRSAKDSGFPGRFRPYSPEARPTTAAGTTLDRLLTDVKKKLKHAKKFWSTLPDTVCVGERIAPSDDCWNGTAKSRYESVVMSSGLANQVSNPDVEVDITKPDMVIRRQIAVLKEMTSWLKAAYTGNDISFTSDDAGSGEESGSGCDSPSCEGDGDIYFSTPAPGKPRILKTPEVEQASSGTRLAPCSLALALASLLLTLLTLQTR

Target ORF information:

RefSeq Version	NM_131860.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glypican 4 (gpc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131860.2

ORF Insert Sequence:

ATGAAGATGA TCGTTGTGTT TACAGTATGT ATGTCTGTGG TGGTTTTGGC GTCGGCTCAG 
GCTGATCAGA AGTCAAAGAA CTGCAATGAA GTCAGAACTG CCTACAGTTC CAAAGGTTTC 
AACGTCAACG ATGTTCCCAA CAAAGGAGTG CAGGGAGCCC ATCTGAAGGT GTGTCCTCAA 
GGTTTCTCCT GCTGTACTCT GGAGATGGAG GAGAAGTTGA GTCAGCTGAG CCGCGTAGAC 
CTGAAAGTTC CCGTTCACCA GCTCAGCTCC AACCTGCAGA GCACCTTCAC CCAGAGACAC 
CGTCACTTTG ACCAGTTCTT CAGAGAGCTG TTGGACAATG CAGAGAAGTC TCTCAATCAC 
ATGTTTGTGC GGACGTACGG CTTGATGTAC GTCAAGAACA AAGAGCTCTT TGAGGGTTTC 
TTCAGTGATC TGCGCCGCTA CTACAGCCAC GGCAGCAGTG AGGTCAATCT GGACGACATG 
CTGGCCGAGT TCTGGTCTGA ACTGCTGGAG CGCATGTTTC GACTGGTCAA CGTCCAGTAC 
GAGTTCAGCG ACTCCTACAT GGAGTGTGTC AGCAGGCACA CGGACCAGCT CAAACCCTTC 
GGAGACGTTC CACGCAAGCT CCGTCTGCAG CTGACCCGCT CCTTCATTGC TGTTCGCGCC 
TTCACTCGTG GCCTCACACT CATGCCTGAT GTGGTCCGCA AAGTCTCAAC GGTGAGCGCC 
TCACCGAGCT GTGTGCGCGC ATCGATGAAG ATGTTATACT GCCCGTACTG TAGCGGTCAG 
GTGGCGCTGA AGCCCTGCAA GAACTACTGT CTGAATGTCA TGAGGGGCTG TCTGGCCAAT 
CAGGCCGATC TGGACACAGA GTGGAATAAC TTCCTTGACT CCATGTTAGG TCTCGCTGAG 
AGGCTGGAGG GACCCTTTAA CTTCGAGTCG GTCGTGGACC CCATCGATGT GAAGATTTCA 
GATGCTATCA TGAACATGCA GGAGAACAGC ATGCAGGTCT CACAGAAGGT GTTTCAGGGT 
TGTGGGCAGC CCAAACTCAG CATGGGCTTC CGCAGCCGGA GGTCTGCTAA AGACTCGGGC 
TTCCCTGGCC GTTTTAGACC GTACAGCCCT GAGGCCAGAC CCACCACTGC TGCTGGCACC 
ACCCTGGACC GACTGCTGAC GGATGTCAAG AAGAAACTGA AGCATGCCAA GAAGTTCTGG 
TCCACCCTGC CGGACACAGT GTGTGTTGGA GAAAGGATCG CCCCCAGTGA TGATTGCTGG 
AATGGTACAG CTAAAAGCAG GTACGAATCT GTGGTTATGA GCAGCGGTTT AGCCAATCAG 
GTGTCTAATC CAGACGTGGA GGTTGACATC ACCAAACCAG ACATGGTGAT CCGGCGGCAG 
ATCGCAGTCC TGAAGGAAAT GACCAGCTGG CTGAAAGCTG CTTACACCGG CAACGACATC 
TCATTTACCT CCGATGATGC CGGCAGCGGA GAGGAAAGCG GAAGCGGCTG CGACTCTCCC 
TCTTGCGAAG GCGACGGAGA TATTTACTTC TCCACCCCGG CACCTGGTAA ACCTCGCATC 
CTCAAGACTC CGGAGGTCGA ACAGGCCTCC AGCGGCACAC GGTTGGCACC CTGCAGCCTG 
GCACTGGCCC TGGCCTCCCT TCTGCTCACC CTGCTTACTC TCCAAACAAG ATAA