mitfb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mitfb
Entrez Gene ID	114833
Full Name	microphthalmia-associated transcription factor b
General protein information	Preferred Names microphthalmia-associated transcription factor b Names microphthalmia-associated transcription factor Mitf1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131847.2	NP_571922.2	microphthalmia-associated transcription factor
XM_021469718.1	XP_021325393.1	microphthalmia-associated transcription factor isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MITF	NP_937802.1
Canis lupus familiaris (dog)	MITF	NP_001003337.1
Xenopus tropicalis (tropical clawed frog)	mitf	NP_001093747.1
Pan troglodytes (chimpanzee)	MITF	XP_001138876.1
Bos taurus (cattle)	MITF	NP_001001150.1
Mus musculus (house mouse)	Mitf	NP_001106669.1
	MITF	XP_001086496.1
Gallus gallus (chicken)	MITF	NP_990360.1
Danio rerio (zebrafish)	mitfb	NP_571922.2
Rattus norvegicus (Norway rat)	Mitf	NP_001178018.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish exposure to environmentally relevant concentration of depleted uranium impairs progeny development at the molecular and histological levels.
Armant O, Gombeau K, Murat El Houdigui S, Floriani M, Camilleri V, Cavalie I, Adam-Guillermin C
PloS one12(5)e0177932(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Omeprazole inhibits melanin biosynthesis in melan-a cells and zebrafish.
Baek SH, Lee SH
Experimental dermatology25(3)239-41(2016 Mar)

Sesamol decreases melanin biosynthesis in melanocyte cells and zebrafish: Possible involvement of MITF via the intracellular cAMP and p38/JNK signalling pathways.
Baek SH, Lee SH
Experimental dermatology24(10)761-6(2015 Oct)

Differential evolution of duplicated medakafish mitf genes.
Li M, Zhu F, Hong Y
International journal of biological sciences9(5)496-508(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that one or more Otx targets in addition to mitfa and mitfb, possibly another mitf family member, are necessary for development of the retinal pigmented epithelium in zebrafish.
Title: Otx but not Mitf transcription factors are required for zebrafish retinal pigment epithelium development.

The following mitfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mitfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36111	NM_131847.2 Latest version!	Danio rerio melanocyte inducing transcription factor b (mitfb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa151417	XM_021469718.1 Latest version!	Danio rerio microphthalmia-associated transcription factor b (mitfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mitfb ( NM_131847.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571922.2 Danio rerio melanocyte inducing transcription factor b (mitfb), mRNA.

Price & Availability↑

CloneID	ODa36111
Clone ID Related Accession (Same CDS sequence)	NM_131847.2
Accession Version	NM_131847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	microphthalmia-associated transcription factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN105118.1. On Dec 22, 2011 this sequence version replaced NM_131847.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN105118.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTCGG AATCGGGGAT CGTGCCCGAC TTTGAAGTCG GAGATGATTT TCACGAAGAA 
CCGAAAACGT ATTACGAGCT GAAGAGTCAG CCGCTGCAAA ACAGTAATCC CTCAGAGCAG 
CAGCATGGCT CCTGTAAGCC CCCGCTGGGC TCGTCTCGAG TCCTGCTCCG CCAGCAGCTG 
ATGCGTGAGC AGCTCCAGCA GCAGGAGCGC CGAGAGCAGC AGAAGCGGCA GATCTCCATC 
ACACACAGCC CCGCCATCAA CGTCAGCCAT CCCTGCGGCC CGCCCAGCGC CGCACAGGTG 
CCCATGGAGG TGCTGAAGGT TCAGACTCAT CTTGAAAACC CCACCAAGTA CCACATCCAG 
CAGGCTCAGC GGCAGCAGGT GAAGGCATAT CTGTCCACCA CTCTGGGAGG AAAGCAGGCG 
GTGAGTTTGC CGTGTCCTAG CCAGGCATCT GATCACGGGG GAATGCCTCC GGGGCCGGGA 
AACAGCGCCC CCAACAGTCC CATGGCCTTA CTGACCCTCA ACCCCAACTG CGAGAAAGAG 
ATGGACGATG TCATTGACGA CATCATTGGT TTGGAATCAA GTTACAGTGA TGACATCATG 
GGACTGTCGC TGGACCCCGG ACTTCAGATG GCAAACACGA TCCCGGTGTC TGCGAATCTG 
CTGGACATGT ACAGTAACCC CGGTCTGCCT CCTCCAGGAA TCTCCATCAG TAACTCGTGT 
CCTGCCAACA TGACCAGCGT TAAAAGGGAA TTCTCTGTGA CTCCATCTCC AGCCCTGATG 
CACATGATGG ACAAGCCCAG CTCCTGCAGC AAGTTCGAGA GCTACCAGAG GCCTGAAGGC 
TTCCCTGTGG AGGTAGAAGT TCGGGCTCTC GCAAAGGAAC GGCAAAAAAA GGACAACCAC 
AACTTAATCG AGAGGCGACG GAGATTTAAC ATTAATGATC GAATCAAAGA ACTGGGCACT 
CTGATTCCCA AATCAAATGA CCCGGACATG CGCTGGAACA AGGGCACGAT TCTGAAGGCG 
TCGGTGGATT ACATCAGGAA GCTGCAGCGA GAGCAGCACC GCACTAAAGA GCTGGAGAAC 
CGGCAGAAGA AGCTGGAGCA CACAAACCGC AACCTCTTGC TCAGGATACA GGAGCTGGAG 
ATTCAGGCTC GAGCTCACGG TCTGGCCATG GCTTCATCAG CTCTGTGTGC ATCAGAACTC 
GCGGCGCGGG CCATCAAACA GGAGCCACTA CTGGGCGACT GCAGTCAGGA TATGTACACA 
AGCACCCACG CGCACCCGTC CTGCGCCGAC ATGAGCCGCT CCAGCACCCT GGACCTGAAC 
AACGGCACAA TCAGCTTCAG CGACACACAC CTTTCAGACA CACACGCCGC CAAACTGGAC 
GACATCCTGA TGGACGAAAC ACTGACGTCT GGAGCCACAA ATGAGTCTCT TATCTCCGCC 
GCCTCCAACG AAAGCACGCA CAAAGACAGC ATGAACATGG AGGAGAACCA GCATGCTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571922.2
CDS	93..1595
Translation	MQSESGIVPDFEVGDDFHEEPKTYYELKSQPLQNSNPSEQQHGSCKPPLGSSRVLLRQQLMREQLQQQERREQQKRQISITHSPAINVSHPCGPPSAAQVPMEVLKVQTHLENPTKYHIQQAQRQQVKAYLSTTLGGKQAVSLPCPSQASDHGGMPPGPGNSAPNSPMALLTLNPNCEKEMDDVIDDIIGLESSYSDDIMGLSLDPGLQMANTIPVSANLLDMYSNPGLPPPGISISNSCPANMTSVKREFSVTPSPALMHMMDKPSSCSKFESYQRPEGFPVEVEVRALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQHRTKELENRQKKLEHTNRNLLLRIQELEIQARAHGLAMASSALCASELAARAIKQEPLLGDCSQDMYTSTHAHPSCADMSRSSTLDLNNGTISFSDTHLSDTHAAKLDDILMDETLTSGATNESLISAASNESTHKDSMNMEENQHAC

Target ORF information:

RefSeq Version	NM_131847.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio melanocyte inducing transcription factor b (mitfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131847.2

ORF Insert Sequence:

ATGCAGTCGG AATCGGGGAT CGTGCCCGAC TTTGAAGTCG GAGATGATTT TCACGAAGAA 
CCGAAAACGT ATTACGAGCT GAAGAGTCAG CCGCTGCAAA ACAGTAATCC CTCAGAGCAG 
CAGCATGGCT CCTGTAAGCC CCCGCTGGGC TCGTCTCGAG TCCTGCTCCG CCAGCAGCTG 
ATGCGTGAGC AGCTCCAGCA GCAGGAGCGC CGAGAGCAGC AGAAGCGGCA GATCTCCATC 
ACACACAGCC CCGCCATCAA CGTCAGCCAT CCCTGCGGCC CGCCCAGCGC CGCACAGGTG 
CCCATGGAGG TGCTGAAGGT TCAGACTCAT CTTGAAAACC CCACCAAGTA CCACATCCAG 
CAGGCTCAGC GGCAGCAGGT GAAGGCATAT CTGTCCACCA CTCTGGGAGG AAAGCAGGCG 
GTGAGTTTGC CGTGTCCTAG CCAGGCATCT GATCACGGGG GAATGCCTCC GGGGCCGGGA 
AACAGCGCCC CCAACAGTCC CATGGCCTTA CTGACCCTCA ACCCCAACTG CGAGAAAGAG 
ATGGACGATG TCATTGACGA CATCATTGGT TTGGAATCAA GTTACAGTGA TGACATCATG 
GGACTGTCGC TGGACCCCGG ACTTCAGATG GCAAACACGA TCCCGGTGTC TGCGAATCTG 
CTGGACATGT ACAGTAACCC CGGTCTGCCT CCTCCAGGAA TCTCCATCAG TAACTCGTGT 
CCTGCCAACA TGACCAGCGT TAAAAGGGAA TTCTCTGTGA CTCCATCTCC AGCCCTGATG 
CACATGATGG ACAAGCCCAG CTCCTGCAGC AAGTTCGAGA GCTACCAGAG GCCTGAAGGC 
TTCCCTGTGG AGGTAGAAGT TCGGGCTCTC GCAAAGGAAC GGCAAAAAAA GGACAACCAC 
AACTTAATCG AGAGGCGACG GAGATTTAAC ATTAATGATC GAATCAAAGA ACTGGGCACT 
CTGATTCCCA AATCAAATGA CCCGGACATG CGCTGGAACA AGGGCACGAT TCTGAAGGCG 
TCGGTGGATT ACATCAGGAA GCTGCAGCGA GAGCAGCACC GCACTAAAGA GCTGGAGAAC 
CGGCAGAAGA AGCTGGAGCA CACAAACCGC AACCTCTTGC TCAGGATACA GGAGCTGGAG 
ATTCAGGCTC GAGCTCACGG TCTGGCCATG GCTTCATCAG CTCTGTGTGC ATCAGAACTC 
GCGGCGCGGG CCATCAAACA GGAGCCACTA CTGGGCGACT GCAGTCAGGA TATGTACACA 
AGCACCCACG CGCACCCGTC CTGCGCCGAC ATGAGCCGCT CCAGCACCCT GGACCTGAAC 
AACGGCACAA TCAGCTTCAG CGACACACAC CTTTCAGACA CACACGCCGC CAAACTGGAC 
GACATCCTGA TGGACGAAAC ACTGACGTCT GGAGCCACAA ATGAGTCTCT TATCTCCGCC 
GCCTCCAACG AAAGCACGCA CAAAGACAGC ATGAACATGG AGGAGAACCA GCATGCTTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mitfb ( XM_021469718.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325393.1 Danio rerio microphthalmia-associated transcription factor b (mitfb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151417
Clone ID Related Accession (Same CDS sequence)	XM_021469718.1
Accession Version	XM_021469718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	microphthalmia-associated transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGG GGCCGGGAAA CAGCGCCCCC AACAGTCCCA TGGCCTTACT GACCCTCAAC 
CCCAACTGCG AGAAAGAGAT GGACGATGTC ATTGACGACA TCATTGGTTT GGAATCAAGT 
TACAGTGATG ACATCATGGG ACTGTCGCTG GACCCCGGAC TTCAGATGGC AAACACGATC 
CCGGTGTCTG CGAATCTGCT GGACATGTAC AGTAACCCCG GTCTGCCTCC TCCAGGAATC 
TCCATCAGTA ACTCCTGTCC TGCCAACATG ACCAGCGTTA AAAGGGAATT CTCTGTGACT 
CCATCTCCAG CCCTGATGCA CATGATGGAC AAGCCCAGCT CCTGCAGCAA GTTCGAGAGC 
TACCAGAGGC CTGAAGGCTT CCCTGTGGAG GTAGAAGTTC GGGCTCTCGC AAAGGAACGG 
CAAAAAAAGG ACAACCACAA CTTAATCGAG AGGCGACGGA GATTCAACAT TAATGATCGA 
ATCAAAGAAC TGGGCACTCT GATTCCCAAA TCAAACGACC CGGACATGCG CTGGAACAAG 
GGCACGATTC TGAAGGCGTC GGTGGATTAC ATCAGGAAAC TGCAGCGAGA GCAGCACCGC 
ACTAAAGAGC TGGAGAACCG GCAGAAGAAG CTGGAGCACA CAAACCGCAA CCTCTTGCTC 
AGGATACAGG AGCTGGAGAT TCAGGCTCGA GCTCACGGTC TGGCCATGGC TTCATCAGCT 
CTGTGTGCAT CAGAACTCGC GGCGCGGGCC ATCAAACAGG AGCCACTACT GGGCGACTGC 
AGTCAGGATA TGTACACAAG CACCCACGCG CACCCGTCCT GCGCCGACAT GAGCCGCTCC 
AGCACCCTGG ACCTGAACAA CGGCACAATC AGCTTCAGCG ACACACACCT TTCAGACACA 
CACGCCGCCA AACTGGACGA CATCCTGATG GACGAAACAC TGACGTCTGG AGCCACAAAT 
GAGTCTCTTA TCTCCGCCGC CTCCAACGAA AGCACGCACA AAGACAGCAT GAACATGGAG 
GAGAACCAGC ATGCTTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325393.1
CDS	581..1621
Translation	MPPGPGNSAPNSPMALLTLNPNCEKEMDDVIDDIIGLESSYSDDIMGLSLDPGLQMANTIPVSANLLDMYSNPGLPPPGISISNSCPANMTSVKREFSVTPSPALMHMMDKPSSCSKFESYQRPEGFPVEVEVRALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQHRTKELENRQKKLEHTNRNLLLRIQELEIQARAHGLAMASSALCASELAARAIKQEPLLGDCSQDMYTSTHAHPSCADMSRSSTLDLNNGTISFSDTHLSDTHAAKLDDILMDETLTSGATNESLISAASNESTHKDSMNMEENQHAC

Target ORF information:

RefSeq Version	XM_021469718.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microphthalmia-associated transcription factor b (mitfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469718.1

ORF Insert Sequence:

ATGCCTCCGG GGCCGGGAAA CAGCGCCCCC AACAGTCCCA TGGCCTTACT GACCCTCAAC 
CCCAACTGCG AGAAAGAGAT GGACGATGTC ATTGACGACA TCATTGGTTT GGAATCAAGT 
TACAGTGATG ACATCATGGG ACTGTCGCTG GACCCCGGAC TTCAGATGGC AAACACGATC 
CCGGTGTCTG CGAATCTGCT GGACATGTAC AGTAACCCCG GTCTGCCTCC TCCAGGAATC 
TCCATCAGTA ACTCCTGTCC TGCCAACATG ACCAGCGTTA AAAGGGAATT CTCTGTGACT 
CCATCTCCAG CCCTGATGCA CATGATGGAC AAGCCCAGCT CCTGCAGCAA GTTCGAGAGC 
TACCAGAGGC CTGAAGGCTT CCCTGTGGAG GTAGAAGTTC GGGCTCTCGC AAAGGAACGG 
CAAAAAAAGG ACAACCACAA CTTAATCGAG AGGCGACGGA GATTCAACAT TAATGATCGA 
ATCAAAGAAC TGGGCACTCT GATTCCCAAA TCAAACGACC CGGACATGCG CTGGAACAAG 
GGCACGATTC TGAAGGCGTC GGTGGATTAC ATCAGGAAAC TGCAGCGAGA GCAGCACCGC 
ACTAAAGAGC TGGAGAACCG GCAGAAGAAG CTGGAGCACA CAAACCGCAA CCTCTTGCTC 
AGGATACAGG AGCTGGAGAT TCAGGCTCGA GCTCACGGTC TGGCCATGGC TTCATCAGCT 
CTGTGTGCAT CAGAACTCGC GGCGCGGGCC ATCAAACAGG AGCCACTACT GGGCGACTGC 
AGTCAGGATA TGTACACAAG CACCCACGCG CACCCGTCCT GCGCCGACAT GAGCCGCTCC 
AGCACCCTGG ACCTGAACAA CGGCACAATC AGCTTCAGCG ACACACACCT TTCAGACACA 
CACGCCGCCA AACTGGACGA CATCCTGATG GACGAAACAC TGACGTCTGG AGCCACAAAT 
GAGTCTCTTA TCTCCGCCGC CTCCAACGAA AGCACGCACA AAGACAGCAT GAACATGGAG 
GAGAACCAGC ATGCTTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish exposure to environmentally relevant concentration of depleted uranium impairs progeny development at the molecular and histological levels.
PloS one12(5)e0177932(2017)
Armant O,Gombeau K,Murat El Houdigui S,Floriani M,Camilleri V,Cavalie I,Adam-Guillermin C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Omeprazole inhibits melanin biosynthesis in melan-a cells and zebrafish.
Experimental dermatology25(3)239-41(2016 Mar)
Baek SH,Lee SH

Sesamol decreases melanin biosynthesis in melanocyte cells and zebrafish: Possible involvement of MITF via the intracellular cAMP and p38/JNK signalling pathways.
Experimental dermatology24(10)761-6(2015 Oct)
Baek SH,Lee SH

Differential evolution of duplicated medakafish mitf genes.
International journal of biological sciences9(5)496-508(2013)
Li M,Zhu F,Hong Y