arel1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arel1
Entrez Gene ID	100332031
Full Name	apoptosis resistant E3 ubiquitin protein ligase 1
General protein information	Preferred Names apoptosis resistant E3 ubiquitin protein ligase 1 Names apoptosis-resistant E3 ubiquitin protein ligase 1 zmp:0000001299
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002663035.5	XP_002663081.2	apoptosis-resistant E3 ubiquitin protein ligase 1
XM_002663035.6	XP_002663081.2	apoptosis-resistant E3 ubiquitin protein ligase 1

Pathways from BioSystems

Homologies

	AREL1	XP_001094796.1
Gallus gallus (chicken)	KIAA0317	XP_004941854.1
Danio rerio (zebrafish)	LOC100332031	XP_002663081.2
Homo sapiens (human)	AREL1	NP_001034568.1
Pan troglodytes (chimpanzee)	AREL1	XP_001157027.1
Canis lupus familiaris (dog)	AREL1	XP_547907.3
Bos taurus (cattle)	AREL1	NP_001069633.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009516	XP_310176.2
Mus musculus (house mouse)	Arel1	NP_835166.3
Rattus norvegicus (Norway rat)	Arel1	NP_001100214.1
Xenopus tropicalis (tropical clawed frog)	arel1	NP_001011393.1
Drosophila melanogaster (fruit fly)	CG4238	NP_001259895.1

Gene Ontology

The following arel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22907	XM_002663035.6 Latest version!	Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa22907	XM_002663035.5	Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arel1 ( XM_002663035.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663081.2 Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.

Price & Availability↑

CloneID	ODa22907
Clone ID Related Accession (Same CDS sequence)	XM_002663035.5 , XM_002663035.6
Accession Version	XM_002663035.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	apoptosis-resistant E3 ubiquitin protein ligase 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663035.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCC TGTTTTTGCT GACCTTCGTC TTCTGTTCAG TCTCGTGGGT TTTCTTCTGG 
GAGGTGCGCT GGAAGAAAGG GAAGGAAAGT CAGATTGAAG AATGGCTCCA GGGGCACGGC 
CTGTCCGAAT ACAAGTATTT GTTTGAAGAT GTTCAGACCC TGGAGGAGCT GAGTTTATGC 
GTGCTGTCCC GACTGGAGGA AGTGGTAAAG GAAAAGCGTC ACTGGAGGGA GATCATCGAA 
GCCCACGTGC AGCTGCTCAG AGACTTTGCC TTTCAGGAGT GGCTTTGTTC TCAGAGCCTG 
GAGCATTACT ATCACACTTT GAAGACTTTG GGATGCACTA ATTTGGATGA TCTAGCCCAG 
TTTGACAGCC AACTTCAGCT CTCTCTGGCA GCGTGGGGCT ATTACTACGA GGATTACATC 
AAACTGTCCA CAGGCGTTAG GGTTCTCCAG GCTTCAAAGG GAAGCAGAGA TCAAGATTAC 
GAGGTCCAGC TGGTGCACAG CCTTGCTGAG AGGAGGCTGA ATGAGAAGTG GTCTTTCGCA 
GGAGCTCTGA TCTTTGGCTG TACCATTGCC CTGTGCTTTC TGATAAGGGA CCTCATGTTT 
TACGTGATTG GTGGAATAAC TGTTTCCATC ATCGCATTCG TCTTTACCAT CAAGTTTCTG 
TGCGAGCTTG CTGCTCGTGT GGTCAGCTTC TTACAGAATG ATGACCCTGC CCGACATGGA 
GACCGCAGTA TTTACGACTA TGTGCGGGGT AATTATTTGG ACCCACGCTC CTGCAAAGTC 
TCCTGGGACT GGAAAGAGCC ACAGGAAGTG GGTCAAACCA TGAGTTTCCG AGTGCAGCTT 
TTCTACAAAA ATGGCCAGCC GTTTCCTGCA CACAGACCTG TGGGACTGAG GGTGAACATC 
ACTCACATAG AGCTGGCCTT AGAAGTCCCT GTTACCCAGG AGGTCCTTCA AGAGCCTGAG 
TCTAATGTCG TCAAAGTGGC CTTCACTGTG CGGAAGGCTG GACGCTATGA GGTAGCTGTC 
AAACTCGGAG GACTGAATGT GGCCTACAGT CCTTACTACA AGATATTTCA ACCAGGTACA 
GTGGTCCCAT CCAAAACAAA GATAGCCTAC CACTTTTCAA CACTGGTGCT GACTTACGGC 
CAGCAGCACA CGCTGCAGAT CGAGCCTCGG GATGAGTACG GCAACCCCAC CAGCAACTCC 
GTCTCACTCG TAGATGAGGT CAACTACAGC GTGCATGTCC ATCCATTGGG CACAGTGGAG 
GAGGAGAGCT CAGAGGACTT CTACAGCACG TCAGTGTCCG CAAACAAGCC TCAATGTCAA 
GTTTTGATGA GGCTTACTTT CAAGAAGAAA GGCTGCTTTA GAGCCTGCAT CTCGTACAGG 
GACCAGCCCC TCAGAAACGG GGAGTTTGAC ATCATTGTAC TCAGCGAAAA TGAGAAGAAC 
TGTGTGGAGA AAAACGTGTC TACCCCAGGC ATAAGCATCT ACTTTGAGGC CTACCTCTAC 
AACATGGGCA CTTACAGTAG TTGTCCTTGG CAGATTTTAG CATCTTCTCA ACTAGCCCTG 
CAGAGACGGC CCTCCATGGG TGAAGAGGAG GAGGAGCATG ACTCCCCTGT AGAGGGCCAG 
TCAGAGAAAA TCAGGAAACC AAAAAAGGTC TATTGCTACA TCTCACCAAA GCAATTTTCT 
GTGAAGGAGT TTTACCTGAA AATTATTCCT TGGCGCCTGT TCACTTTTCG TGTGTGTCCC 
GGAACCAAGT TTACGTACCA TGGACCAGAC CCTGTTCATA AATACTTAAC CCTGGTGGTA 
GATGATGGGA TCCAGCCTCC AGTAGAGCTC AGCTGTAAAG ACCGCAACAT AATGGCTGCC 
ACCTTTATCC GCTTTCTCCA CAAGAACATT GGAGGATCAG AGACTTTTCA GGATAAAGTG 
AACTTTTTTC AAAGGGAGCT AAGGCACATC CACTCCAAGA GACCTCGCAC TAAAACTAGC 
CTGAAAATCA GTCGGCACTC TCTGCTGGAG TCGTCTCTGA AAGCCACACG GAACTTTTCT 
GTGTCTGACT GGAGCAAGAA CTTTGAAGTT GTGTTCCAGG ATGAGGAAGC TCTCGACTGG 
GGAGGGCCAA GGCGTGAGTG GTTTGAGCTC GTCTGTAAAG CTCTGTTTGA TACCAATAAC 
CAGCTTTTCA CACGCTTTAG TGACAACAAT CAAGGCCTGG TCCATCCAAA CGCTGAACGC 
CCCCCTCACC TGAGAGTGAA AATGTATGAG TTTGCAGGCC GGGTTGTGGG GAAATGTTTA 
TATGAATCCG CTTTGGGCGG GTCCTACAAG CAGTTGGTAC GAGCCCGATT CACCCGCTCC 
TTCTTGGCTC AGATAATTGG ATTGCGAATG AACTATAAGT ACTTTGAAAC TGATGACGAG 
GAATTTTACA AAACCAAGGT CTGCTTCATT CTCAACAATG ATGTCAGCGA GATGGACCTG 
CTGTTTGCAG AGGAAAAATA CAGCAAGTCT GGACACCTGG AAAAGGTGGT GGAACTGATA 
CCTGGTGGTG CTCAAATTGC TGTGACCAAT GAGAATAAAA ATCACTATTT AAACTTGTTG 
GCCCAGTACA GACTAGCCAG TCAAGTGAGA GAGGAAGTCG AGCACTTCCT TAAAGGTTTG 
AACGAACTGG TCCCTGAAAA CCTTCTGGCC ATATTTGATG AGAATGAACT GGAGTTGTTG 
ATGTGTGGCA CAGGAGATAT AAATGTCCAG GACTTTAAAG CTCATGCAGT AATTGTTGGA 
GGGTCATGGC ATTTCAGAGA AAAGGTGATG AAGTGGTTCT GGGCGGTGGT ATCATCCTTC 
ACACAGGAAG AATTGGCGCG ATTGCTGCAG TTCACCACCG GCTCCTCACA GCTTCCACCT 
GGTGGCTTCA ACACGCTCTG CCCTTCCTTC CAAATCATAG CAGCTCCCAC ACACAGCACG 
CTGCCCACCG CACACACCTG TTTTAACCAG CTGTGCCTCC CAACATATGA CTCCTACGAG 
GAGCTCCACA AGCTGCTGAA GCTGGCCATC AGTGAGGGGA GCGAGGGCTT TGGGATGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663081.2
CDS	181..3243
Translation	MDRLFLLTFVFCSVSWVFFWEVRWKKGKESQIEEWLQGHGLSEYKYLFEDVQTLEELSLCVLSRLEEVVKEKRHWREIIEAHVQLLRDFAFQEWLCSQSLEHYYHTLKTLGCTNLDDLAQFDSQLQLSLAAWGYYYEDYIKLSTGVRVLQASKGSRDQDYEVQLVHSLAERRLNEKWSFAGALIFGCTIALCFLIRDLMFYVIGGITVSIIAFVFTIKFLCELAARVVSFLQNDDPARHGDRSIYDYVRGNYLDPRSCKVSWDWKEPQEVGQTMSFRVQLFYKNGQPFPAHRPVGLRVNITHIELALEVPVTQEVLQEPESNVVKVAFTVRKAGRYEVAVKLGGLNVAYSPYYKIFQPGTVVPSKTKIAYHFSTLVLTYGQQHTLQIEPRDEYGNPTSNSVSLVDEVNYSVHVHPLGTVEEESSEDFYSTSVSANKPQCQVLMRLTFKKKGCFRACISYRDQPLRNGEFDIIVLSENEKNCVEKNVSTPGISIYFEAYLYNMGTYSSCPWQILASSQLALQRRPSMGEEEEEHDSPVEGQSEKIRKPKKVYCYISPKQFSVKEFYLKIIPWRLFTFRVCPGTKFTYHGPDPVHKYLTLVVDDGIQPPVELSCKDRNIMAATFIRFLHKNIGGSETFQDKVNFFQRELRHIHSKRPRTKTSLKISRHSLLESSLKATRNFSVSDWSKNFEVVFQDEEALDWGGPRREWFELVCKALFDTNNQLFTRFSDNNQGLVHPNAERPPHLRVKMYEFAGRVVGKCLYESALGGSYKQLVRARFTRSFLAQIIGLRMNYKYFETDDEEFYKTKVCFILNNDVSEMDLLFAEEKYSKSGHLEKVVELIPGGAQIAVTNENKNHYLNLLAQYRLASQVREEVEHFLKGLNELVPENLLAIFDENELELLMCGTGDINVQDFKAHAVIVGGSWHFREKVMKWFWAVVSSFTQEELARLLQFTTGSSQLPPGGFNTLCPSFQIIAAPTHSTLPTAHTCFNQLCLPTYDSYEELHKLLKLAISEGSEGFGML

Target ORF information:

RefSeq Version	XM_002663035.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663035.5

ORF Insert Sequence:

ATGGACCGCC TGTTTTTGCT GACCTTCGTC TTCTGTTCAG TCTCGTGGGT TTTCTTCTGG 
GAGGTGCGCT GGAAGAAAGG GAAGGAAAGT CAGATTGAAG AATGGCTCCA GGGGCACGGC 
CTGTCCGAAT ACAAGTATTT GTTTGAAGAT GTTCAGACCC TGGAGGAGCT GAGTTTATGC 
GTGCTGTCCC GACTGGAGGA AGTGGTAAAG GAAAAGCGTC ACTGGAGGGA GATCATCGAA 
GCCCACGTGC AGCTGCTCAG AGACTTTGCC TTTCAGGAGT GGCTTTGTTC TCAGAGCCTG 
GAGCATTACT ATCACACTTT GAAGACTTTG GGATGCACTA ATTTGGATGA TCTAGCCCAG 
TTTGACAGCC AACTTCAGCT CTCTCTGGCA GCGTGGGGCT ATTACTACGA GGATTACATC 
AAACTGTCCA CAGGCGTTAG GGTTCTCCAG GCTTCAAAGG GAAGCAGAGA TCAAGATTAC 
GAGGTCCAGC TGGTGCACAG CCTTGCTGAG AGGAGGCTGA ATGAGAAGTG GTCTTTCGCA 
GGAGCTCTGA TCTTTGGCTG TACCATTGCC CTGTGCTTTC TGATAAGGGA CCTCATGTTT 
TACGTGATTG GTGGAATAAC TGTTTCCATC ATCGCATTCG TCTTTACCAT CAAGTTTCTG 
TGCGAGCTTG CTGCTCGTGT GGTCAGCTTC TTACAGAATG ATGACCCTGC CCGACATGGA 
GACCGCAGTA TTTACGACTA TGTGCGGGGT AATTATTTGG ACCCACGCTC CTGCAAAGTC 
TCCTGGGACT GGAAAGAGCC ACAGGAAGTG GGTCAAACCA TGAGTTTCCG AGTGCAGCTT 
TTCTACAAAA ATGGCCAGCC GTTTCCTGCA CACAGACCTG TGGGACTGAG GGTGAACATC 
ACTCACATAG AGCTGGCCTT AGAAGTCCCT GTTACCCAGG AGGTCCTTCA AGAGCCTGAG 
TCTAATGTCG TCAAAGTGGC CTTCACTGTG CGGAAGGCTG GACGCTATGA GGTAGCTGTC 
AAACTCGGAG GACTGAATGT GGCCTACAGT CCTTACTACA AGATATTTCA ACCAGGTACA 
GTGGTCCCAT CCAAAACAAA GATAGCCTAC CACTTTTCAA CACTGGTGCT GACTTACGGC 
CAGCAGCACA CGCTGCAGAT CGAGCCTCGG GATGAGTACG GCAACCCCAC CAGCAACTCC 
GTCTCACTCG TAGATGAGGT CAACTACAGC GTGCATGTCC ATCCATTGGG CACAGTGGAG 
GAGGAGAGCT CAGAGGACTT CTACAGCACG TCAGTGTCCG CAAACAAGCC TCAATGTCAA 
GTTTTGATGA GGCTTACTTT CAAGAAGAAA GGCTGCTTTA GAGCCTGCAT CTCGTACAGG 
GACCAGCCCC TCAGAAACGG GGAGTTTGAC ATCATTGTAC TCAGCGAAAA TGAGAAGAAC 
TGTGTGGAGA AAAACGTGTC TACCCCAGGC ATAAGCATCT ACTTTGAGGC CTACCTCTAC 
AACATGGGCA CTTACAGTAG TTGTCCTTGG CAGATTTTAG CATCTTCTCA ACTAGCCCTG 
CAGAGACGGC CCTCCATGGG TGAAGAGGAG GAGGAGCATG ACTCCCCTGT AGAGGGCCAG 
TCAGAGAAAA TCAGGAAACC AAAAAAGGTC TATTGCTACA TCTCACCAAA GCAATTTTCT 
GTGAAGGAGT TTTACCTGAA AATTATTCCT TGGCGCCTGT TCACTTTTCG TGTGTGTCCC 
GGAACCAAGT TTACGTACCA TGGACCAGAC CCTGTTCATA AATACTTAAC CCTGGTGGTA 
GATGATGGGA TCCAGCCTCC AGTAGAGCTC AGCTGTAAAG ACCGCAACAT AATGGCTGCC 
ACCTTTATCC GCTTTCTCCA CAAGAACATT GGAGGATCAG AGACTTTTCA GGATAAAGTG 
AACTTTTTTC AAAGGGAGCT AAGGCACATC CACTCCAAGA GACCTCGCAC TAAAACTAGC 
CTGAAAATCA GTCGGCACTC TCTGCTGGAG TCGTCTCTGA AAGCCACACG GAACTTTTCT 
GTGTCTGACT GGAGCAAGAA CTTTGAAGTT GTGTTCCAGG ATGAGGAAGC TCTCGACTGG 
GGAGGGCCAA GGCGTGAGTG GTTTGAGCTC GTCTGTAAAG CTCTGTTTGA TACCAATAAC 
CAGCTTTTCA CACGCTTTAG TGACAACAAT CAAGGCCTGG TCCATCCAAA CGCTGAACGC 
CCCCCTCACC TGAGAGTGAA AATGTATGAG TTTGCAGGCC GGGTTGTGGG GAAATGTTTA 
TATGAATCCG CTTTGGGCGG GTCCTACAAG CAGTTGGTAC GAGCCCGATT CACCCGCTCC 
TTCTTGGCTC AGATAATTGG ATTGCGAATG AACTATAAGT ACTTTGAAAC TGATGACGAG 
GAATTTTACA AAACCAAGGT CTGCTTCATT CTCAACAATG ATGTCAGCGA GATGGACCTG 
CTGTTTGCAG AGGAAAAATA CAGCAAGTCT GGACACCTGG AAAAGGTGGT GGAACTGATA 
CCTGGTGGTG CTCAAATTGC TGTGACCAAT GAGAATAAAA ATCACTATTT AAACTTGTTG 
GCCCAGTACA GACTAGCCAG TCAAGTGAGA GAGGAAGTCG AGCACTTCCT TAAAGGTTTG 
AACGAACTGG TCCCTGAAAA CCTTCTGGCC ATATTTGATG AGAATGAACT GGAGTTGTTG 
ATGTGTGGCA CAGGAGATAT AAATGTCCAG GACTTTAAAG CTCATGCAGT AATTGTTGGA 
GGGTCATGGC ATTTCAGAGA AAAGGTGATG AAGTGGTTCT GGGCGGTGGT ATCATCCTTC 
ACACAGGAAG AATTGGCGCG ATTGCTGCAG TTCACCACCG GCTCCTCACA GCTTCCACCT 
GGTGGCTTCA ACACGCTCTG CCCTTCCTTC CAAATCATAG CAGCTCCCAC ACACAGCACG 
CTGCCCACCG CACACACCTG TTTTAACCAG CTGTGCCTCC CAACATATGA CTCCTACGAG 
GAGCTCCACA AGCTGCTGAA GCTGGCCATC AGTGAGGGGA GCGAGGGCTT TGGGATGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arel1 ( XM_002663035.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663081.2 Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.

Price & Availability↑

CloneID	ODa22907
Clone ID Related Accession (Same CDS sequence)	XM_002663035.5 , XM_002663035.6
Accession Version	XM_002663035.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	apoptosis-resistant E3 ubiquitin protein ligase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663035.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCC TGTTTTTGCT GACCTTCGTC TTCTGTTCAG TCTCGTGGGT TTTCTTCTGG 
GAGGTGCGCT GGAAGAAAGG GAAGGAAAGT CAGATTGAAG AATGGCTCCA GGGGCACGGC 
CTGTCCGAAT ACAAGTATTT GTTTGAAGAT GTTCAGACCC TGGAGGAGCT GAGTTTATGC 
GTGCTGTCCC GACTGGAGGA AGTGGTAAAG GAAAAGCGTC ACTGGAGGGA GATCATCGAA 
GCCCACGTGC AGCTGCTCAG AGACTTTGCC TTTCAGGAGT GGCTTTGTTC TCAGAGCCTG 
GAGCATTACT ATCACACTTT GAAGACTTTG GGATGCACTA ATTTGGATGA TCTAGCCCAG 
TTTGACAGCC AACTTCAGCT CTCTCTGGCA GCGTGGGGCT ATTACTACGA GGATTACATC 
AAACTGTCCA CAGGCGTTAG GGTTCTCCAG GCTTCAAAGG GAAGCAGAGA TCAAGATTAC 
GAGGTCCAGC TGGTGCACAG CCTTGCTGAG AGGAGGCTGA ATGAGAAGTG GTCTTTCGCA 
GGAGCTCTGA TCTTTGGCTG TACCATTGCC CTGTGCTTTC TGATAAGGGA CCTCATGTTT 
TACGTGATTG GTGGAATAAC TGTTTCCATC ATCGCATTCG TCTTTACCAT CAAGTTTCTG 
TGCGAGCTTG CTGCTCGTGT GGTCAGCTTC TTACAGAATG ATGACCCTGC CCGACATGGA 
GACCGCAGTA TTTACGACTA TGTGCGGGGT AATTATTTGG ACCCACGCTC CTGCAAAGTC 
TCCTGGGACT GGAAAGAGCC ACAGGAAGTG GGTCAAACCA TGAGTTTCCG AGTGCAGCTT 
TTCTACAAAA ATGGCCAGCC GTTTCCTGCA CACAGACCTG TGGGACTGAG GGTGAACATC 
ACTCACATAG AGCTGGCCTT AGAAGTCCCT GTTACCCAGG AGGTCCTTCA AGAGCCTGAG 
TCTAATGTCG TCAAAGTGGC CTTCACTGTG CGGAAGGCTG GACGCTATGA GGTAGCTGTC 
AAACTCGGAG GACTGAATGT GGCCTACAGT CCTTACTACA AGATATTTCA ACCAGGTACA 
GTGGTCCCAT CCAAAACAAA GATAGCCTAC CACTTTTCAA CACTGGTGCT GACTTACGGC 
CAGCAGCACA CGCTGCAGAT CGAGCCTCGG GATGAGTACG GCAACCCCAC CAGCAACTCC 
GTCTCACTCG TAGATGAGGT CAACTACAGC GTGCATGTCC ATCCATTGGG CACAGTGGAG 
GAGGAGAGCT CAGAGGACTT CTACAGCACG TCAGTGTCCG CAAACAAGCC TCAATGTCAA 
GTTTTGATGA GGCTTACTTT CAAGAAGAAA GGCTGCTTTA GAGCCTGCAT CTCGTACAGG 
GACCAGCCCC TCAGAAACGG GGAGTTTGAC ATCATTGTAC TCAGCGAAAA TGAGAAGAAC 
TGTGTGGAGA AAAACGTGTC TACCCCAGGC ATAAGCATCT ACTTTGAGGC CTACCTCTAC 
AACATGGGCA CTTACAGTAG TTGTCCTTGG CAGATTTTAG CATCTTCTCA ACTAGCCCTG 
CAGAGACGGC CCTCCATGGG TGAAGAGGAG GAGGAGCATG ACTCCCCTGT AGAGGGCCAG 
TCAGAGAAAA TCAGGAAACC AAAAAAGGTC TATTGCTACA TCTCACCAAA GCAATTTTCT 
GTGAAGGAGT TTTACCTGAA AATTATTCCT TGGCGCCTGT TCACTTTTCG TGTGTGTCCC 
GGAACCAAGT TTACGTACCA TGGACCAGAC CCTGTTCATA AATACTTAAC CCTGGTGGTA 
GATGATGGGA TCCAGCCTCC AGTAGAGCTC AGCTGTAAAG ACCGCAACAT AATGGCTGCC 
ACCTTTATCC GCTTTCTCCA CAAGAACATT GGAGGATCAG AGACTTTTCA GGATAAAGTG 
AACTTTTTTC AAAGGGAGCT AAGGCACATC CACTCCAAGA GACCTCGCAC TAAAACTAGC 
CTGAAAATCA GTCGGCACTC TCTGCTGGAG TCGTCTCTGA AAGCCACACG GAACTTTTCT 
GTGTCTGACT GGAGCAAGAA CTTTGAAGTT GTGTTCCAGG ATGAGGAAGC TCTCGACTGG 
GGAGGGCCAA GGCGTGAGTG GTTTGAGCTC GTCTGTAAAG CTCTGTTTGA TACCAATAAC 
CAGCTTTTCA CACGCTTTAG TGACAACAAT CAAGGCCTGG TCCATCCAAA CGCTGAACGC 
CCCCCTCACC TGAGAGTGAA AATGTATGAG TTTGCAGGCC GGGTTGTGGG GAAATGTTTA 
TATGAATCCG CTTTGGGCGG GTCCTACAAG CAGTTGGTAC GAGCCCGATT CACCCGCTCC 
TTCTTGGCTC AGATAATTGG ATTGCGAATG AACTATAAGT ACTTTGAAAC TGATGACGAG 
GAATTTTACA AAACCAAGGT CTGCTTCATT CTCAACAATG ATGTCAGCGA GATGGACCTG 
CTGTTTGCAG AGGAAAAATA CAGCAAGTCT GGACACCTGG AAAAGGTGGT GGAACTGATA 
CCTGGTGGTG CTCAAATTGC TGTGACCAAT GAGAATAAAA ATCACTATTT AAACTTGTTG 
GCCCAGTACA GACTAGCCAG TCAAGTGAGA GAGGAAGTCG AGCACTTCCT TAAAGGTTTG 
AACGAACTGG TCCCTGAAAA CCTTCTGGCC ATATTTGATG AGAATGAACT GGAGTTGTTG 
ATGTGTGGCA CAGGAGATAT AAATGTCCAG GACTTTAAAG CTCATGCAGT AATTGTTGGA 
GGGTCATGGC ATTTCAGAGA AAAGGTGATG AAGTGGTTCT GGGCGGTGGT ATCATCCTTC 
ACACAGGAAG AATTGGCGCG ATTGCTGCAG TTCACCACCG GCTCCTCACA GCTTCCACCT 
GGTGGCTTCA ACACGCTCTG CCCTTCCTTC CAAATCATAG CAGCTCCCAC ACACAGCACG 
CTGCCCACCG CACACACCTG TTTTAACCAG CTGTGCCTCC CAACATATGA CTCCTACGAG 
GAGCTCCACA AGCTGCTGAA GCTGGCCATC AGTGAGGGGA GCGAGGGCTT TGGGATGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663081.2
CDS	176..3238
Translation	MDRLFLLTFVFCSVSWVFFWEVRWKKGKESQIEEWLQGHGLSEYKYLFEDVQTLEELSLCVLSRLEEVVKEKRHWREIIEAHVQLLRDFAFQEWLCSQSLEHYYHTLKTLGCTNLDDLAQFDSQLQLSLAAWGYYYEDYIKLSTGVRVLQASKGSRDQDYEVQLVHSLAERRLNEKWSFAGALIFGCTIALCFLIRDLMFYVIGGITVSIIAFVFTIKFLCELAARVVSFLQNDDPARHGDRSIYDYVRGNYLDPRSCKVSWDWKEPQEVGQTMSFRVQLFYKNGQPFPAHRPVGLRVNITHIELALEVPVTQEVLQEPESNVVKVAFTVRKAGRYEVAVKLGGLNVAYSPYYKIFQPGTVVPSKTKIAYHFSTLVLTYGQQHTLQIEPRDEYGNPTSNSVSLVDEVNYSVHVHPLGTVEEESSEDFYSTSVSANKPQCQVLMRLTFKKKGCFRACISYRDQPLRNGEFDIIVLSENEKNCVEKNVSTPGISIYFEAYLYNMGTYSSCPWQILASSQLALQRRPSMGEEEEEHDSPVEGQSEKIRKPKKVYCYISPKQFSVKEFYLKIIPWRLFTFRVCPGTKFTYHGPDPVHKYLTLVVDDGIQPPVELSCKDRNIMAATFIRFLHKNIGGSETFQDKVNFFQRELRHIHSKRPRTKTSLKISRHSLLESSLKATRNFSVSDWSKNFEVVFQDEEALDWGGPRREWFELVCKALFDTNNQLFTRFSDNNQGLVHPNAERPPHLRVKMYEFAGRVVGKCLYESALGGSYKQLVRARFTRSFLAQIIGLRMNYKYFETDDEEFYKTKVCFILNNDVSEMDLLFAEEKYSKSGHLEKVVELIPGGAQIAVTNENKNHYLNLLAQYRLASQVREEVEHFLKGLNELVPENLLAIFDENELELLMCGTGDINVQDFKAHAVIVGGSWHFREKVMKWFWAVVSSFTQEELARLLQFTTGSSQLPPGGFNTLCPSFQIIAAPTHSTLPTAHTCFNQLCLPTYDSYEELHKLLKLAISEGSEGFGML

Target ORF information:

RefSeq Version	XM_002663035.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio apoptosis resistant E3 ubiquitin protein ligase 1 (arel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663035.6

ORF Insert Sequence:

ATGGACCGCC TGTTTTTGCT GACCTTCGTC TTCTGTTCAG TCTCGTGGGT TTTCTTCTGG 
GAGGTGCGCT GGAAGAAAGG GAAGGAAAGT CAGATTGAAG AATGGCTCCA GGGGCACGGC 
CTGTCCGAAT ACAAGTATTT GTTTGAAGAT GTTCAGACCC TGGAGGAGCT GAGTTTATGC 
GTGCTGTCCC GACTGGAGGA AGTGGTAAAG GAAAAGCGTC ACTGGAGGGA GATCATCGAA 
GCCCACGTGC AGCTGCTCAG AGACTTTGCC TTTCAGGAGT GGCTTTGTTC TCAGAGCCTG 
GAGCATTACT ATCACACTTT GAAGACTTTG GGATGCACTA ATTTGGATGA TCTAGCCCAG 
TTTGACAGCC AACTTCAGCT CTCTCTGGCA GCGTGGGGCT ATTACTACGA GGATTACATC 
AAACTGTCCA CAGGCGTTAG GGTTCTCCAG GCTTCAAAGG GAAGCAGAGA TCAAGATTAC 
GAGGTCCAGC TGGTGCACAG CCTTGCTGAG AGGAGGCTGA ATGAGAAGTG GTCTTTCGCA 
GGAGCTCTGA TCTTTGGCTG TACCATTGCC CTGTGCTTTC TGATAAGGGA CCTCATGTTT 
TACGTGATTG GTGGAATAAC TGTTTCCATC ATCGCATTCG TCTTTACCAT CAAGTTTCTG 
TGCGAGCTTG CTGCTCGTGT GGTCAGCTTC TTACAGAATG ATGACCCTGC CCGACATGGA 
GACCGCAGTA TTTACGACTA TGTGCGGGGT AATTATTTGG ACCCACGCTC CTGCAAAGTC 
TCCTGGGACT GGAAAGAGCC ACAGGAAGTG GGTCAAACCA TGAGTTTCCG AGTGCAGCTT 
TTCTACAAAA ATGGCCAGCC GTTTCCTGCA CACAGACCTG TGGGACTGAG GGTGAACATC 
ACTCACATAG AGCTGGCCTT AGAAGTCCCT GTTACCCAGG AGGTCCTTCA AGAGCCTGAG 
TCTAATGTCG TCAAAGTGGC CTTCACTGTG CGGAAGGCTG GACGCTATGA GGTAGCTGTC 
AAACTCGGAG GACTGAATGT GGCCTACAGT CCTTACTACA AGATATTTCA ACCAGGTACA 
GTGGTCCCAT CCAAAACAAA GATAGCCTAC CACTTTTCAA CACTGGTGCT GACTTACGGC 
CAGCAGCACA CGCTGCAGAT CGAGCCTCGG GATGAGTACG GCAACCCCAC CAGCAACTCC 
GTCTCACTCG TAGATGAGGT CAACTACAGC GTGCATGTCC ATCCATTGGG CACAGTGGAG 
GAGGAGAGCT CAGAGGACTT CTACAGCACG TCAGTGTCCG CAAACAAGCC TCAATGTCAA 
GTTTTGATGA GGCTTACTTT CAAGAAGAAA GGCTGCTTTA GAGCCTGCAT CTCGTACAGG 
GACCAGCCCC TCAGAAACGG GGAGTTTGAC ATCATTGTAC TCAGCGAAAA TGAGAAGAAC 
TGTGTGGAGA AAAACGTGTC TACCCCAGGC ATAAGCATCT ACTTTGAGGC CTACCTCTAC 
AACATGGGCA CTTACAGTAG TTGTCCTTGG CAGATTTTAG CATCTTCTCA ACTAGCCCTG 
CAGAGACGGC CCTCCATGGG TGAAGAGGAG GAGGAGCATG ACTCCCCTGT AGAGGGCCAG 
TCAGAGAAAA TCAGGAAACC AAAAAAGGTC TATTGCTACA TCTCACCAAA GCAATTTTCT 
GTGAAGGAGT TTTACCTGAA AATTATTCCT TGGCGCCTGT TCACTTTTCG TGTGTGTCCC 
GGAACCAAGT TTACGTACCA TGGACCAGAC CCTGTTCATA AATACTTAAC CCTGGTGGTA 
GATGATGGGA TCCAGCCTCC AGTAGAGCTC AGCTGTAAAG ACCGCAACAT AATGGCTGCC 
ACCTTTATCC GCTTTCTCCA CAAGAACATT GGAGGATCAG AGACTTTTCA GGATAAAGTG 
AACTTTTTTC AAAGGGAGCT AAGGCACATC CACTCCAAGA GACCTCGCAC TAAAACTAGC 
CTGAAAATCA GTCGGCACTC TCTGCTGGAG TCGTCTCTGA AAGCCACACG GAACTTTTCT 
GTGTCTGACT GGAGCAAGAA CTTTGAAGTT GTGTTCCAGG ATGAGGAAGC TCTCGACTGG 
GGAGGGCCAA GGCGTGAGTG GTTTGAGCTC GTCTGTAAAG CTCTGTTTGA TACCAATAAC 
CAGCTTTTCA CACGCTTTAG TGACAACAAT CAAGGCCTGG TCCATCCAAA CGCTGAACGC 
CCCCCTCACC TGAGAGTGAA AATGTATGAG TTTGCAGGCC GGGTTGTGGG GAAATGTTTA 
TATGAATCCG CTTTGGGCGG GTCCTACAAG CAGTTGGTAC GAGCCCGATT CACCCGCTCC 
TTCTTGGCTC AGATAATTGG ATTGCGAATG AACTATAAGT ACTTTGAAAC TGATGACGAG 
GAATTTTACA AAACCAAGGT CTGCTTCATT CTCAACAATG ATGTCAGCGA GATGGACCTG 
CTGTTTGCAG AGGAAAAATA CAGCAAGTCT GGACACCTGG AAAAGGTGGT GGAACTGATA 
CCTGGTGGTG CTCAAATTGC TGTGACCAAT GAGAATAAAA ATCACTATTT AAACTTGTTG 
GCCCAGTACA GACTAGCCAG TCAAGTGAGA GAGGAAGTCG AGCACTTCCT TAAAGGTTTG 
AACGAACTGG TCCCTGAAAA CCTTCTGGCC ATATTTGATG AGAATGAACT GGAGTTGTTG 
ATGTGTGGCA CAGGAGATAT AAATGTCCAG GACTTTAAAG CTCATGCAGT AATTGTTGGA 
GGGTCATGGC ATTTCAGAGA AAAGGTGATG AAGTGGTTCT GGGCGGTGGT ATCATCCTTC 
ACACAGGAAG AATTGGCGCG ATTGCTGCAG TTCACCACCG GCTCCTCACA GCTTCCACCT 
GGTGGCTTCA ACACGCTCTG CCCTTCCTTC CAAATCATAG CAGCTCCCAC ACACAGCACG 
CTGCCCACCG CACACACCTG TTTTAACCAG CTGTGCCTCC CAACATATGA CTCCTACGAG 
GAGCTCCACA AGCTGCTGAA GCTGGCCATC AGTGAGGGGA GCGAGGGCTT TGGGATGCTA 
TAA