ALP3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALP3
Entrez Gene ID	100330410
Full Name	alkaline phosphatase 3
General protein information	Preferred Names alkaline phosphatase 3 Names alkaline phosphatase, tissue-nonspecific isozyme
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278839.1	NP_001265768.1	alkaline phosphatase, tissue-nonspecific isozyme precursor
XM_017359193.1	XP_017214682.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALPL	NP_789828.2
Canis lupus familiaris (dog)	ALPL	NP_001184066.1
Rattus norvegicus (Norway rat)	Alpl	NP_037191.1
Gallus gallus (chicken)	ALPL	NP_990691.1
Drosophila melanogaster (fruit fly)	CG8147	NP_649897.4
Homo sapiens (human)	ALPL	NP_000469.3
	ALPL	XP_001109717.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001684	XP_321411.3
Pan troglodytes (chimpanzee)	ALPL	XP_003307915.1
Xenopus tropicalis (tropical clawed frog)	LOC100485467	XP_002943485.2
Mus musculus (house mouse)	Alpl	NP_001274101.1
Danio rerio (zebrafish)	alp3	NP_001265768.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates.
Yang Y, Wandler AM, Postlethwait JH, Guillemin K
Frontiers in immunology3314(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33527	NM_001278839.1 Latest version!	Danio rerio alkaline phosphatase 3 (alp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa55049	XM_017359193.1 Latest version!	Danio rerio alkaline phosphatase 3 (alp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALP3 ( NM_001278839.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001265768.1 Danio rerio alkaline phosphatase 3 (alp3), mRNA.

Price & Availability↑

CloneID	ODa33527
Clone ID Related Accession (Same CDS sequence)	NM_001278839.1
Accession Version	NM_001278839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Danio rerio(zebrafish)
Product	alkaline phosphatase, tissue-nonspecific isozyme precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JX847418.1. On Jul 17, 2013 this sequence version replaced XM_003201677.1. ##Evidence-Data-START## Transcript exon combination :: JX847418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTGCTG TCCGTGTGTC CGTCGTCTGT TTTCTGCTGA CCATCACTTC TGGATCAGTT 
GAAGAGGAGA ATCCAGAGTT CTGGAGGAAG TCGGCTCAAA ACACACTGCG TTCGGCTCTG 
AGCCGGAAAA TCAACACTAA TGTGGCCAAA AACATTGTGC TGTTTCTGGG AGACGGTATG 
GGAGTGACGA CCATCACAGC CGCGCGGATC CTGAAGGGTC AGTTACAGAA ACACTCAGGC 
GAGGAGACCG TCATGAATAT GGACACATTC CCCAATGTGG GTCTTGCGAA GGTGTATTCA 
GTGGATTTTC AGATTCCTGA CAGCGCAGCG ACGGCTACAG CGTATCTGTG CGGAGTCAAA 
ACCAACCTGA ACACTGTGGG TGTGAGCGCA GCAGCACGTA ACGGAGTGTG TCGATCACAG 
AAGGGCAACG AGGTCACGTC CATCCTCAGA TGGGCCAAAG ACGCAGGTAA GTCAGTAGGA 
ATAGTAACGA CGACTCGTGT CCAGCATGCG ACTCCAGCAG CTTCATTCGC TCACAGCGCC 
AGCAGGACCT GGTACAGCGA TGCGGATCTG CCTGCGTCTG CAGCTACTGA AGGCTGTGTG 
GACATCGCTA CACAACTACT ACACAACATC GATATTGACG TGATCATCGG AGGAGGCCGT 
AAGTACATGA CACCTAAAGG TTTCCCCGAT CCAGAGTATC CGTCAGACGC GAGTGCTCAG 
GGCCAGCGGA GAGACGGCAG AAACCTCATT CAGGACTGGA TACAGATGAA GGAAGGAAAG 
GTTGCCAGAT ATGTGTGGAA TAAGACAGAT TTTGACGCTG TGGATCCTGA AAAGACTGAT 
TATCTGATGG CTCTGTTTGA GCCGGCTGAT CTGAGGTTTG ATGTGGAGCG AGATCACAGC 
ATGGACCCCT CCATCTCTGA AACCACCGAC AAAGCCATTC AGATCCTCAA GAAAAACCCC 
AAAGGATTCT TCCTGCTGGT CGAAGGTGGT CGTATTGATC AGGGTCATCA CTCCAGTCGG 
GCGTCAGCCG CTCTGCATGA AGCTGTGGCT CTGGATGAGG CGGTTTCTCG TGGACTGGAG 
CTCACTGATG AAGAGGAGAC GCTGACCATC GTCACTGCTG ACCACTCACA CGCATTCACT 
TTCAACGGAT ACCCTTTCAG AGGAAACAGC ATTCTGGGTA AATCTCCAAT CTTCGCCTCT 
GACTTTTTAC CATACACAAC ACTGATGTAC GGGAATGGTC CGGGACACAA AATCACCAAC 
AACAAGCGTC CAGACATACG CAAAGTTGAC ACTGCCGATA AGGACTACAT CCAGCAGTCG 
GCCGTCCCGC TGGACTCAGA GACCCACGGG GGTGAGGATG TGGCAGTGTT CGCTCGCGGG 
CCCATGGCAC ATCTCTTCCA GGGCGTTTAT GAGCAGAACT ACATCCCGCA CGCCATGGCT 
TACGCCGCAT GCATTGGTGA AAATCAGCAA CACTGCGCTA TTACTGCAAA ACCAGACGAA 
AACCCAGACA CATCCACTGA CCCGGGAAAC TCTGCCCAAA ACATGCCCGA CTATACCTTC 
ACCATCCTGA TGATCAGCAC ACTGATGAGG ATTTTACTGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265768.1
CDS	1..1605
Translation	MFAVRVSVVCFLLTITSGSVEEENPEFWRKSAQNTLRSALSRKINTNVAKNIVLFLGDGMGVTTITAARILKGQLQKHSGEETVMNMDTFPNVGLAKVYSVDFQIPDSAATATAYLCGVKTNLNTVGVSAAARNGVCRSQKGNEVTSILRWAKDAGKSVGIVTTTRVQHATPAASFAHSASRTWYSDADLPASAATEGCVDIATQLLHNIDIDVIIGGGRKYMTPKGFPDPEYPSDASAQGQRRDGRNLIQDWIQMKEGKVARYVWNKTDFDAVDPEKTDYLMALFEPADLRFDVERDHSMDPSISETTDKAIQILKKNPKGFFLLVEGGRIDQGHHSSRASAALHEAVALDEAVSRGLELTDEEETLTIVTADHSHAFTFNGYPFRGNSILGKSPIFASDFLPYTTLMYGNGPGHKITNNKRPDIRKVDTADKDYIQQSAVPLDSETHGGEDVAVFARGPMAHLFQGVYEQNYIPHAMAYAACIGENQQHCAITAKPDENPDTSTDPGNSAQNMPDYTFTILMISTLMRILLH

Target ORF information:

RefSeq Version	NM_001278839.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alkaline phosphatase 3 (alp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278839.1

ORF Insert Sequence:

ATGTTTGCTG TCCGTGTGTC CGTCGTCTGT TTTCTGCTGA CCATCACTTC TGGATCAGTT 
GAAGAGGAGA ATCCAGAGTT CTGGAGGAAG TCGGCTCAAA ACACACTGCG TTCGGCTCTG 
AGCCGGAAAA TCAACACTAA TGTGGCCAAA AACATTGTGC TGTTTCTGGG AGACGGTATG 
GGAGTGACGA CCATCACAGC CGCGCGGATC CTGAAGGGTC AGTTACAGAA ACACTCAGGC 
GAGGAGACCG TCATGAATAT GGACACATTC CCCAATGTGG GTCTTGCGAA GGTGTATTCA 
GTGGATTTTC AGATTCCTGA CAGCGCAGCG ACGGCTACAG CGTATCTGTG CGGAGTCAAA 
ACCAACCTGA ACACTGTGGG TGTGAGCGCA GCAGCACGTA ACGGAGTGTG TCGATCACAG 
AAGGGCAACG AGGTCACGTC CATCCTCAGA TGGGCCAAAG ACGCAGGTAA GTCAGTAGGA 
ATAGTAACGA CGACTCGTGT CCAGCATGCG ACTCCAGCAG CTTCATTCGC TCACAGCGCC 
AGCAGGACCT GGTACAGCGA TGCGGATCTG CCTGCGTCTG CAGCTACTGA AGGCTGTGTG 
GACATCGCTA CACAACTACT ACACAACATC GATATTGACG TGATCATCGG AGGAGGCCGT 
AAGTACATGA CACCTAAAGG TTTCCCCGAT CCAGAGTATC CGTCAGACGC GAGTGCTCAG 
GGCCAGCGGA GAGACGGCAG AAACCTCATT CAGGACTGGA TACAGATGAA GGAAGGAAAG 
GTTGCCAGAT ATGTGTGGAA TAAGACAGAT TTTGACGCTG TGGATCCTGA AAAGACTGAT 
TATCTGATGG CTCTGTTTGA GCCGGCTGAT CTGAGGTTTG ATGTGGAGCG AGATCACAGC 
ATGGACCCCT CCATCTCTGA AACCACCGAC AAAGCCATTC AGATCCTCAA GAAAAACCCC 
AAAGGATTCT TCCTGCTGGT CGAAGGTGGT CGTATTGATC AGGGTCATCA CTCCAGTCGG 
GCGTCAGCCG CTCTGCATGA AGCTGTGGCT CTGGATGAGG CGGTTTCTCG TGGACTGGAG 
CTCACTGATG AAGAGGAGAC GCTGACCATC GTCACTGCTG ACCACTCACA CGCATTCACT 
TTCAACGGAT ACCCTTTCAG AGGAAACAGC ATTCTGGGTA AATCTCCAAT CTTCGCCTCT 
GACTTTTTAC CATACACAAC ACTGATGTAC GGGAATGGTC CGGGACACAA AATCACCAAC 
AACAAGCGTC CAGACATACG CAAAGTTGAC ACTGCCGATA AGGACTACAT CCAGCAGTCG 
GCCGTCCCGC TGGACTCAGA GACCCACGGG GGTGAGGATG TGGCAGTGTT CGCTCGCGGG 
CCCATGGCAC ATCTCTTCCA GGGCGTTTAT GAGCAGAACT ACATCCCGCA CGCCATGGCT 
TACGCCGCAT GCATTGGTGA AAATCAGCAA CACTGCGCTA TTACTGCAAA ACCAGACGAA 
AACCCAGACA CATCCACTGA CCCGGGAAAC TCTGCCCAAA ACATGCCCGA CTATACCTTC 
ACCATCCTGA TGATCAGCAC ACTGATGAGG ATTTTACTGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALP3 ( XM_017359193.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214682.1 Danio rerio alkaline phosphatase 3 (alp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55049
Clone ID Related Accession (Same CDS sequence)	XM_017359193.1
Accession Version	XM_017359193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATGG ACACATTCCC CAATGTGGGT CTTGCGAAGG TGTATTCAGT GGATTTTCAG 
ATTCCTGACA GCGCAGCGAC GGCTACAGCG TATCTGTGCG GAGTCAAAAC CAACCTGAAC 
ACTGTGGGTG TGAGCGCAGC AGCACGTAAC GGAGTGTGTC GATCACAGAA GGGCAACGAG 
GTCACGTCCA TCCTCAGATG GGCCAAAGAC GCAGGTAAGT CAGTAGGAAT AGTAACGACG 
ACTCGTGTCC AGCATGCGAC TCCAGCAGCT TCATTCGCTC ACAGTGCCAG CAGGACCTGG 
TACAGCGATG CGGATCTGCC TGCGTCTGCA GCTACTGAAG GCTGTGTGGA CATCGCTACA 
CAACTACTAC ACAACATCGA TATTGATGTG ATCATCGGAG GAGGCCGTAA GTACATGACA 
CCTAAAGGTT TCCCCGATCC AGAGTATCCG TCAGACGCGA GTGCTCAGGG CCAGCGGAGA 
GACGGCAGAA ACCTCATTCA GGACTGGATA CAGATGAAGG AAGGAAAGGT TGCCAGATAT 
GTGTGGAATA AGACAGATTT TGACGCTGTG GATCCTGAAA AGACTGATTA TCTGATGGCT 
CTGTTTGAGC CGGCTGATCT GAGGTTTGAT GTGGAGCGAG ATCACAGCAT GGACCCCTCC 
ATCTCTGAAA CCACCGACAA AGCCATTCAG ATCCTCAAGA AAAACCCCAA AGGATTCTTC 
CTGCTGGTCG AAGGTGGTCG TATTGATCAG GGTCATCACT CCAGTCGGGC GTCAGCCGCT 
CTGCATGAAG CTGTGGCTCT GGATGAGGCG GTTTCTCGTG GACTGGAGCT CACTGATGAA 
GAGGAGACGC TGACCATCGT CACTGCTGAC CACTCACACG CATTCACTTT CAACGGATAC 
CCTTTCAGAG GAAACAGCAT TCTGGGTAAA TCTCCAATCT TCGCCTCTGA CTTTTTACCA 
TACACAACAC TGATGTACGG GAATGGTCCG GGACACAAAA TCACCAACAA CAAGCGTCCA 
GACATACGCA AAGTTGACAC TGCCGATAAG GACTACATCC AGCAGTCGGC CGTCCCGCTG 
GACTCAGAGA CCCACGGGGG TGAGGATGTG GCAGTGTTCG CTCGCGGGCC CATGGCACAT 
CTCTTCCAGG GCGTTTATGA GCAGAACTAC ATCCCGCACG CCATGGCTTA CGCCGCATGC 
ATTGGTGAAA ATCAGCAACA CTGCGCTATT ACTGCAAAAC CAGACGAAAA CCCAGACACA 
TCCACTGACC CGGGAAACTC TGCCCAAAAC ATGCCCGACT ATACCTTCAC CATCCTGATG 
ATCAGCACAC TGATGAGGAT TTTACTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214682.1
CDS	41..1393
Translation	MNMDTFPNVGLAKVYSVDFQIPDSAATATAYLCGVKTNLNTVGVSAAARNGVCRSQKGNEVTSILRWAKDAGKSVGIVTTTRVQHATPAASFAHSASRTWYSDADLPASAATEGCVDIATQLLHNIDIDVIIGGGRKYMTPKGFPDPEYPSDASAQGQRRDGRNLIQDWIQMKEGKVARYVWNKTDFDAVDPEKTDYLMALFEPADLRFDVERDHSMDPSISETTDKAIQILKKNPKGFFLLVEGGRIDQGHHSSRASAALHEAVALDEAVSRGLELTDEEETLTIVTADHSHAFTFNGYPFRGNSILGKSPIFASDFLPYTTLMYGNGPGHKITNNKRPDIRKVDTADKDYIQQSAVPLDSETHGGEDVAVFARGPMAHLFQGVYEQNYIPHAMAYAACIGENQQHCAITAKPDENPDTSTDPGNSAQNMPDYTFTILMISTLMRILLH

Target ORF information:

RefSeq Version	XM_017359193.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alkaline phosphatase 3 (alp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359193.1

ORF Insert Sequence:

ATGAATATGG ACACATTCCC CAATGTGGGT CTTGCGAAGG TGTATTCAGT GGATTTTCAG 
ATTCCTGACA GCGCAGCGAC GGCTACAGCG TATCTGTGCG GAGTCAAAAC CAACCTGAAC 
ACTGTGGGTG TGAGCGCAGC AGCACGTAAC GGAGTGTGTC GATCACAGAA GGGCAACGAG 
GTCACGTCCA TCCTCAGATG GGCCAAAGAC GCAGGTAAGT CAGTAGGAAT AGTAACGACG 
ACTCGTGTCC AGCATGCGAC TCCAGCAGCT TCATTCGCTC ACAGTGCCAG CAGGACCTGG 
TACAGCGATG CGGATCTGCC TGCGTCTGCA GCTACTGAAG GCTGTGTGGA CATCGCTACA 
CAACTACTAC ACAACATCGA TATTGATGTG ATCATCGGAG GAGGCCGTAA GTACATGACA 
CCTAAAGGTT TCCCCGATCC AGAGTATCCG TCAGACGCGA GTGCTCAGGG CCAGCGGAGA 
GACGGCAGAA ACCTCATTCA GGACTGGATA CAGATGAAGG AAGGAAAGGT TGCCAGATAT 
GTGTGGAATA AGACAGATTT TGACGCTGTG GATCCTGAAA AGACTGATTA TCTGATGGCT 
CTGTTTGAGC CGGCTGATCT GAGGTTTGAT GTGGAGCGAG ATCACAGCAT GGACCCCTCC 
ATCTCTGAAA CCACCGACAA AGCCATTCAG ATCCTCAAGA AAAACCCCAA AGGATTCTTC 
CTGCTGGTCG AAGGTGGTCG TATTGATCAG GGTCATCACT CCAGTCGGGC GTCAGCCGCT 
CTGCATGAAG CTGTGGCTCT GGATGAGGCG GTTTCTCGTG GACTGGAGCT CACTGATGAA 
GAGGAGACGC TGACCATCGT CACTGCTGAC CACTCACACG CATTCACTTT CAACGGATAC 
CCTTTCAGAG GAAACAGCAT TCTGGGTAAA TCTCCAATCT TCGCCTCTGA CTTTTTACCA 
TACACAACAC TGATGTACGG GAATGGTCCG GGACACAAAA TCACCAACAA CAAGCGTCCA 
GACATACGCA AAGTTGACAC TGCCGATAAG GACTACATCC AGCAGTCGGC CGTCCCGCTG 
GACTCAGAGA CCCACGGGGG TGAGGATGTG GCAGTGTTCG CTCGCGGGCC CATGGCACAT 
CTCTTCCAGG GCGTTTATGA GCAGAACTAC ATCCCGCACG CCATGGCTTA CGCCGCATGC 
ATTGGTGAAA ATCAGCAACA CTGCGCTATT ACTGCAAAAC CAGACGAAAA CCCAGACACA 
TCCACTGACC CGGGAAACTC TGCCCAAAAC ATGCCCGACT ATACCTTCAC CATCCTGATG 
ATCAGCACAC TGATGAGGAT TTTACTGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates.
Frontiers in immunology3314(2012)
Yang Y,Wandler AM,Postlethwait JH,Guillemin K