ulk2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ulk2
Entrez Gene ID	100329702
Full Name	unc-51 like autophagy activating kinase 2
Synonyms	im:7164951
General protein information	Preferred Names unc-51 like autophagy activating kinase 2 Names serine/threonine-protein kinase ULK2 unc-51-like kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002664615.4	XP_002664661.3	serine/threonine-protein kinase ULK2
XM_002664615.5	XP_002664661.3	serine/threonine-protein kinase ULK2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ulk2	XP_002664661.3
Homo sapiens (human)	ULK2	NP_001136082.1
	ULK2	XP_001099375.2
Mus musculus (house mouse)	Ulk2	NP_038909.3
Rattus norvegicus (Norway rat)	Ulk2	NP_001178574.1
Gallus gallus (chicken)	ULK2	XP_004946752.1
Xenopus tropicalis (tropical clawed frog)	ulk2	XP_004911684.1
Pan troglodytes (chimpanzee)	ULK2	XP_511339.2
Canis lupus familiaris (dog)	ULK2	XP_546644.4

Gene Ontology

The following ulk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ulk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20418	XM_002664615.5 Latest version!	Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa20418	XM_002664615.4	Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ulk2 ( XM_002664615.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664661.3 Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.

Price & Availability↑

CloneID	ODa20418
Clone ID Related Accession (Same CDS sequence)	XM_002664615.4 , XM_002664615.5
Accession Version	XM_002664615.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase ULK2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_002664615.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG TGGGAGATTT TGAATACAGC CGAAAAGACC TCATCGGACA CGGAGCCTTT 
GCGGTCGTCT TCAAGGGAAG ACATAAAAAG AAGACTGATT GGGAGGTCGC CATCAAAAGC 
ATTAACAAAA AGAATCTCTC CAAATCCCAG ATTCTGCTTG GCAAAGAAAT AAAAATTTTA 
AAGGAACTCC AGCATGAAAA CATTGTGGCG CTCTATGATG TCCAGGAAAC GCCAAGTTCT 
GTATTTCTGG TTATGGAGTA CTGCAATGGA GGAGACCTGG CAGATTATTT GCAAGCCAAG 
GGCACTCTGA GAGAGGACAC ACTGAGGGTC TTTCTACAGC AGATTGCAGC CGCCATGCGA 
ATCCTTAACA GCAAGGGGAT CATCCATCGA GACCTCAAAC CCCAGAACAT CCTGCTGTCC 
TACACGGGCC GCAAGAAGTC CAGCATCAAC GGCATACGCA TCAAGATCGC CGACTTTGGT 
TTTGCGAGAT ACCTCCAGAG CAACATGATG GCAGCCACAC TATGTGGATC CCCGATGTAC 
ATGGCCCCGG AGGTCATCAT GTCCCAGAAT TATGATGCCA AAGCTGACCT GTGGAGCATT 
GGGACTGTTA TTTACCAGTG CCTCGTGGGA AAGCCGCCTT TTCAGGCTAA CAGTCCCCAG 
GACTTGAGGA TGTTCTATGA AAAGAACAAG TCTTTGGTGC CAAACATCCC GAGAGAGACA 
TCTCCTCAAC TGGAAGACCT GCTGCTGGGG CTTCTTCAGA GAAACCAGAA AGATCGTATA 
GATTTTGACA CTTTCTTCAG TCATCCTTTC TTGGAACCGA TATCAACTAT CAAAAAATCA 
TGCCCTGTTC CAGTGCCCTC ATGTTCAGGC TTGGTCTCAG ACAGCACATG TGGCAGCTCT 
CCATCCTGCC GTTACGTGTC TCCTCCGTCA CTGCCAGACA TGCAGACTCT CCCTGAAGAC 
GTGTTGTCCT CTCCACCTCT CGGTCCTCCC AACTATCTGC AGCTGTCCAA GGAGTCCGGC 
GGCAGCACCA GCAGCAAAAA CTCCTCCTGT GACACAGATG ACTTTGTGCT GGTGCCGCAT 
CTGTCTGGGG AACAGTCCTA TGACCTTCCA ATGGGGGCAG TGGGTCGCAG GCCATCCAGT 
GAATTTCTCC TGTGTGGAGG GTCACCGCAG CCATCTACTG GACAGACGCC AATGGTGTCA 
CCACGGAGCG AGACCACGCC AATTCCTGTG CCCACACAGG TGCGAAACTA CCAGCGGATC 
AAACAGAACC TCTCCAGCAG CCCAACCACA ACACTTTATG GCTCTCCCAG GTCAGGCACA 
GTGCGTCGCT CCAACACAAG CCCCATGGGC TTCCCTAAAA TGGTGTCTGC TTCCCCAAGC 
CCTGCAGATA CTGTCCAAAC AGTGGGGCGA CGTCTCTCTA CTGGCAGCTC TCGGCCCTAC 
TCACCCTCTC CACTGGTGGG CACGATTCCT GAGCAGCTCG GCCACTGTTG CTGTGGTCAC 
CCTCAGAGCC ATGAACCGCG CAGTCGCAGC TCTTCAGGCG GTTCCCCAGT GCCATCCTCT 
CAGTTACTGG GCGCTCGTCT TCAGAGTGCG CCGACACTGA CGGACATCTA CCAGAGCAAG 
CAGAAGCTCC ACAAGCAGCT GTCTGACCCA GTGCACCCCA CCTCCTCCGC CTATCCCAGC 
AACCACTCTC CTCAGCTCGG CCGGCCCGCA AACCTCGGCA CCTCCCCAAC CAAACACCTC 
GGTTCCTCTC CTCGCACCTC TGATTGGCTG ACAAAATCAC CGCTCCCCAC TATCATTGGC 
TCTCCAACTA AGGTAACAGC TCCATTCAAA ATCCCTAAAA CTCAAGCCTC TTGCAATCTC 
ATGGCCCTGG CCGACAGCCC CATCCCCAAC AAGACTCTGA TGGATGGACG TGAACTTTGT 
GCTCATCACT GTACAGCGTA TCCCAGCAGC AGACAGCCCG CACCTGAGGC TAGCAAGACT 
TCCTTTGGCA GGTCTGTAAG CGCTGGCAGA CTGTCTGAGC CTCCGGTACG GATCACTCTA 
GGAGGACAGC CGTACCAGGG CAGCACAGAC AGCCTCAACA CAGAGAGACC CATGGACACA 
GCCCCGGCCG GGATGTGTGC TTTGGCAGCA GGAGGAGGGA GTCCTCGCAC CGTCGTGTTC 
ACGGTGGGTT CACCCCCCAG CAGCAGCACC CCTCCAACCT GCAGCCATCT GGCCTCTCGC 
CCGCGCGCCA CCTCAGTGGG CTCCAACAGC TCTGCCGGTT CGCTCTGCTC CACCAGTGGT 
AAGGTGTACA TGGGCTCTCC TCCAGGCATG ACCATCGGCA GCTCTCCACC CGGAGCAGAA 
GCAGGTCCCA GCAGCCTCCG TTACGTCCCA TACGGCACCT CTCCACCCAG CTTGGATGGC 
TTCATTACCT TTGAAGCTCC AGAACTGCCA GAGGAGACGC TGATGGAGCG TGAGCACACA 
GACACACTGA TGTACCTGCG GATGATGCTG TCTTTCACGG ACTGTGTGCT GGAGATCGCC 
GCTCTGCGGG CCGGAGGGCC TGATCTGGGT GCATCGGCTG CTTCTCTCTA TCCTCCTCAG 
GACAGTGTGG TGGTGGACCA GATCAGTCAG CTCAGTAGAG AGTGGGGGCA GGTGGAGCAG 
CTGGTGTTGT ATATGAAAGC AGCTCAGCTT CTGGCCTCCT CGCTGCACCT CGCCAAAGCC 
CAGATCAAAT CTGCCAAACT CAACCCGTCT ACTGCCGTCA AACAGGTGGT GAAGAGTCTG 
AACGAGCGCT ACAAGAGCTG TATTTCCCTG TGCCGTCGCC TCACAGACAA ACTCAACCAC 
TTCTTCTCTG ACAAGCAGCG CTTTGTGGAC GAGATCAACA GTGTGACAGC AGAAAAGCTC 
ATTTATAACC ACGCTGTGGA GATGGTGCAG TCGGCCGCTC TGGATGAGAT GTTTCAGCAG 
ACTGAGGACA TCGCTTATCG CTACAACAAA GCCTCCATGC TGCTGGAAGG CCTGACCAAG 
ATCCTCCAGG ACCCAGCTGA TATTGAGAAC GTTATTAAAT ACAAGGCCAG TGTGGACCGG 
AGGATCTCAG CTTTGTGTTA CTGCACCGTC ACCCTGTACG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664661.3
CDS	992..4096
Translation	METVGDFEYSRKDLIGHGAFAVVFKGRHKKKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVALYDVQETPSSVFLVMEYCNGGDLADYLQAKGTLREDTLRVFLQQIAAAMRILNSKGIIHRDLKPQNILLSYTGRKKSSINGIRIKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTVIYQCLVGKPPFQANSPQDLRMFYEKNKSLVPNIPRETSPQLEDLLLGLLQRNQKDRIDFDTFFSHPFLEPISTIKKSCPVPVPSCSGLVSDSTCGSSPSCRYVSPPSLPDMQTLPEDVLSSPPLGPPNYLQLSKESGGSTSSKNSSCDTDDFVLVPHLSGEQSYDLPMGAVGRRPSSEFLLCGGSPQPSTGQTPMVSPRSETTPIPVPTQVRNYQRIKQNLSSSPTTTLYGSPRSGTVRRSNTSPMGFPKMVSASPSPADTVQTVGRRLSTGSSRPYSPSPLVGTIPEQLGHCCCGHPQSHEPRSRSSSGGSPVPSSQLLGARLQSAPTLTDIYQSKQKLHKQLSDPVHPTSSAYPSNHSPQLGRPANLGTSPTKHLGSSPRTSDWLTKSPLPTIIGSPTKVTAPFKIPKTQASCNLMALADSPIPNKTLMDGRELCAHHCTAYPSSRQPAPEASKTSFGRSVSAGRLSEPPVRITLGGQPYQGSTDSLNTERPMDTAPAGMCALAAGGGSPRTVVFTVGSPPSSSTPPTCSHLASRPRATSVGSNSSAGSLCSTSGKVYMGSPPGMTIGSSPPGAEAGPSSLRYVPYGTSPPSLDGFITFEAPELPEETLMEREHTDTLMYLRMMLSFTDCVLEIAALRAGGPDLGASAASLYPPQDSVVVDQISQLSREWGQVEQLVLYMKAAQLLASSLHLAKAQIKSAKLNPSTAVKQVVKSLNERYKSCISLCRRLTDKLNHFFSDKQRFVDEINSVTAEKLIYNHAVEMVQSAALDEMFQQTEDIAYRYNKASMLLEGLTKILQDPADIENVIKYKASVDRRISALCYCTVTLYE

Target ORF information:

RefSeq Version	XM_002664615.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664615.4

ORF Insert Sequence:

ATGGAGACGG TGGGAGATTT TGAATACAGC CGAAAAGACC TCATCGGACA CGGAGCCTTT 
GCGGTCGTCT TCAAGGGAAG ACATAAAAAG AAGACTGATT GGGAGGTCGC CATCAAAAGC 
ATTAACAAAA AGAATCTCTC CAAATCCCAG ATTCTGCTTG GCAAAGAAAT AAAAATTTTA 
AAGGAACTCC AGCATGAAAA CATTGTGGCG CTCTATGATG TCCAGGAAAC GCCAAGTTCT 
GTATTTCTGG TTATGGAGTA CTGCAATGGA GGAGACCTGG CAGATTATTT GCAAGCCAAG 
GGCACTCTGA GAGAGGACAC ACTGAGGGTC TTTCTACAGC AGATTGCAGC CGCCATGCGA 
ATCCTTAACA GCAAGGGGAT CATCCATCGA GACCTCAAAC CCCAGAACAT CCTGCTGTCC 
TACACGGGCC GCAAGAAGTC CAGCATCAAC GGCATACGCA TCAAGATCGC CGACTTTGGT 
TTTGCGAGAT ACCTCCAGAG CAACATGATG GCAGCCACAC TATGTGGATC CCCGATGTAC 
ATGGCCCCGG AGGTCATCAT GTCCCAGAAT TATGATGCCA AAGCTGACCT GTGGAGCATT 
GGGACTGTTA TTTACCAGTG CCTCGTGGGA AAGCCGCCTT TTCAGGCTAA CAGTCCCCAG 
GACTTGAGGA TGTTCTATGA AAAGAACAAG TCTTTGGTGC CAAACATCCC GAGAGAGACA 
TCTCCTCAAC TGGAAGACCT GCTGCTGGGG CTTCTTCAGA GAAACCAGAA AGATCGTATA 
GATTTTGACA CTTTCTTCAG TCATCCTTTC TTGGAACCGA TATCAACTAT CAAAAAATCA 
TGCCCTGTTC CAGTGCCCTC ATGTTCAGGC TTGGTCTCAG ACAGCACATG TGGCAGCTCT 
CCATCCTGCC GTTACGTGTC TCCTCCGTCA CTGCCAGACA TGCAGACTCT CCCTGAAGAC 
GTGTTGTCCT CTCCACCTCT CGGTCCTCCC AACTATCTGC AGCTGTCCAA GGAGTCCGGC 
GGCAGCACCA GCAGCAAAAA CTCCTCCTGT GACACAGATG ACTTTGTGCT GGTGCCGCAT 
CTGTCTGGGG AACAGTCCTA TGACCTTCCA ATGGGGGCAG TGGGTCGCAG GCCATCCAGT 
GAATTTCTCC TGTGTGGAGG GTCACCGCAG CCATCTACTG GACAGACGCC AATGGTGTCA 
CCACGGAGCG AGACCACGCC AATTCCTGTG CCCACACAGG TGCGAAACTA CCAGCGGATC 
AAACAGAACC TCTCCAGCAG CCCAACCACA ACACTTTATG GCTCTCCCAG GTCAGGCACA 
GTGCGTCGCT CCAACACAAG CCCCATGGGC TTCCCTAAAA TGGTGTCTGC TTCCCCAAGC 
CCTGCAGATA CTGTCCAAAC AGTGGGGCGA CGTCTCTCTA CTGGCAGCTC TCGGCCCTAC 
TCACCCTCTC CACTGGTGGG CACGATTCCT GAGCAGCTCG GCCACTGTTG CTGTGGTCAC 
CCTCAGAGCC ATGAACCGCG CAGTCGCAGC TCTTCAGGCG GTTCCCCAGT GCCATCCTCT 
CAGTTACTGG GCGCTCGTCT TCAGAGTGCG CCGACACTGA CGGACATCTA CCAGAGCAAG 
CAGAAGCTCC ACAAGCAGCT GTCTGACCCA GTGCACCCCA CCTCCTCCGC CTATCCCAGC 
AACCACTCTC CTCAGCTCGG CCGGCCCGCA AACCTCGGCA CCTCCCCAAC CAAACACCTC 
GGTTCCTCTC CTCGCACCTC TGATTGGCTG ACAAAATCAC CGCTCCCCAC TATCATTGGC 
TCTCCAACTA AGGTAACAGC TCCATTCAAA ATCCCTAAAA CTCAAGCCTC TTGCAATCTC 
ATGGCCCTGG CCGACAGCCC CATCCCCAAC AAGACTCTGA TGGATGGACG TGAACTTTGT 
GCTCATCACT GTACAGCGTA TCCCAGCAGC AGACAGCCCG CACCTGAGGC TAGCAAGACT 
TCCTTTGGCA GGTCTGTAAG CGCTGGCAGA CTGTCTGAGC CTCCGGTACG GATCACTCTA 
GGAGGACAGC CGTACCAGGG CAGCACAGAC AGCCTCAACA CAGAGAGACC CATGGACACA 
GCCCCGGCCG GGATGTGTGC TTTGGCAGCA GGAGGAGGGA GTCCTCGCAC CGTCGTGTTC 
ACGGTGGGTT CACCCCCCAG CAGCAGCACC CCTCCAACCT GCAGCCATCT GGCCTCTCGC 
CCGCGCGCCA CCTCAGTGGG CTCCAACAGC TCTGCCGGTT CGCTCTGCTC CACCAGTGGT 
AAGGTGTACA TGGGCTCTCC TCCAGGCATG ACCATCGGCA GCTCTCCACC CGGAGCAGAA 
GCAGGTCCCA GCAGCCTCCG TTACGTCCCA TACGGCACCT CTCCACCCAG CTTGGATGGC 
TTCATTACCT TTGAAGCTCC AGAACTGCCA GAGGAGACGC TGATGGAGCG TGAGCACACA 
GACACACTGA TGTACCTGCG GATGATGCTG TCTTTCACGG ACTGTGTGCT GGAGATCGCC 
GCTCTGCGGG CCGGAGGGCC TGATCTGGGT GCATCGGCTG CTTCTCTCTA TCCTCCTCAG 
GACAGTGTGG TGGTGGACCA GATCAGTCAG CTCAGTAGAG AGTGGGGGCA GGTGGAGCAG 
CTGGTGTTGT ATATGAAAGC AGCTCAGCTT CTGGCCTCCT CGCTGCACCT CGCCAAAGCC 
CAGATCAAAT CTGCCAAACT CAACCCGTCT ACTGCCGTCA AACAGGTGGT GAAGAGTCTG 
AACGAGCGCT ACAAGAGCTG TATTTCCCTG TGCCGTCGCC TCACAGACAA ACTCAACCAC 
TTCTTCTCTG ACAAGCAGCG CTTTGTGGAC GAGATCAACA GTGTGACAGC AGAAAAGCTC 
ATTTATAACC ACGCTGTGGA GATGGTGCAG TCGGCCGCTC TGGATGAGAT GTTTCAGCAG 
ACTGAGGACA TCGCTTATCG CTACAACAAA GCCTCCATGC TGCTGGAAGG CCTGACCAAG 
ATCCTCCAGG ACCCAGCTGA TATTGAGAAC GTTATTAAAT ACAAGGCCAG TGTGGACCGG 
AGGATCTCAG CTTTGTGTTA CTGCACCGTC ACCCTGTACG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk2 ( XM_002664615.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664661.3 Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.

Price & Availability↑

CloneID	ODa20418
Clone ID Related Accession (Same CDS sequence)	XM_002664615.4 , XM_002664615.5
Accession Version	XM_002664615.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase ULK2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002664615.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG TGGGAGATTT TGAATACAGC CGAAAAGACC TCATCGGACA CGGAGCCTTT 
GCGGTCGTCT TCAAGGGAAG ACATAAAAAG AAGACTGATT GGGAGGTCGC CATCAAAAGC 
ATTAACAAAA AGAATCTCTC CAAATCCCAG ATTCTGCTTG GCAAAGAAAT AAAAATTTTA 
AAGGAACTCC AGCATGAAAA CATTGTGGCG CTCTATGATG TCCAGGAAAC GCCAAGTTCT 
GTATTTCTGG TTATGGAGTA CTGCAATGGA GGAGACCTGG CAGATTATTT GCAAGCCAAG 
GGCACTCTGA GAGAGGACAC ACTGAGGGTC TTTCTACAGC AGATTGCAGC CGCCATGCGA 
ATCCTTAACA GCAAGGGGAT CATCCATCGA GACCTCAAAC CCCAGAACAT CCTGCTGTCC 
TACACGGGCC GCAAGAAGTC CAGCATCAAC GGCATACGCA TCAAGATCGC CGACTTTGGT 
TTTGCGAGAT ACCTCCAGAG CAACATGATG GCAGCCACAC TATGTGGATC CCCGATGTAC 
ATGGCCCCGG AGGTCATCAT GTCCCAGAAT TATGATGCCA AAGCTGACCT GTGGAGCATT 
GGGACTGTTA TTTACCAGTG CCTCGTGGGA AAGCCGCCTT TTCAGGCTAA CAGTCCCCAG 
GACTTGAGGA TGTTCTATGA AAAGAACAAG TCTTTGGTGC CAAACATCCC GAGAGAGACA 
TCTCCTCAAC TGGAAGACCT GCTGCTGGGG CTTCTTCAGA GAAACCAGAA AGATCGTATA 
GATTTTGACA CTTTCTTCAG TCATCCTTTC TTGGAACCGA TATCAACTAT CAAAAAATCA 
TGCCCTGTTC CAGTGCCCTC ATGTTCAGGC TTGGTCTCAG ACAGCACATG TGGCAGCTCT 
CCATCCTGCC GTTACGTGTC TCCTCCGTCA CTGCCAGACA TGCAGACTCT CCCTGAAGAC 
GTGTTGTCCT CTCCACCTCT CGGTCCTCCC AACTATCTGC AGCTGTCCAA GGAGTCCGGC 
GGCAGCACCA GCAGCAAAAA CTCCTCCTGT GACACAGATG ACTTTGTGCT GGTGCCGCAT 
CTGTCTGGGG AACAGTCCTA TGACCTTCCA ATGGGGGCAG TGGGTCGCAG GCCATCCAGT 
GAATTTCTCC TGTGTGGAGG GTCACCGCAG CCATCTACTG GACAGACGCC AATGGTGTCA 
CCACGGAGCG AGACCACGCC AATTCCTGTG CCCACACAGG TGCGAAACTA CCAGCGGATC 
AAACAGAACC TCTCCAGCAG CCCAACCACA ACACTTTATG GCTCTCCCAG GTCAGGCACA 
GTGCGTCGCT CCAACACAAG CCCCATGGGC TTCCCTAAAA TGGTGTCTGC TTCCCCAAGC 
CCTGCAGATA CTGTCCAAAC AGTGGGGCGA CGTCTCTCTA CTGGCAGCTC TCGGCCCTAC 
TCACCCTCTC CACTGGTGGG CACGATTCCT GAGCAGCTCG GCCACTGTTG CTGTGGTCAC 
CCTCAGAGCC ATGAACCGCG CAGTCGCAGC TCTTCAGGCG GTTCCCCAGT GCCATCCTCT 
CAGTTACTGG GCGCTCGTCT TCAGAGTGCG CCGACACTGA CGGACATCTA CCAGAGCAAG 
CAGAAGCTCC ACAAGCAGCT GTCTGACCCA GTGCACCCCA CCTCCTCCGC CTATCCCAGC 
AACCACTCTC CTCAGCTCGG CCGGCCCGCA AACCTCGGCA CCTCCCCAAC CAAACACCTC 
GGTTCCTCTC CTCGCACCTC TGATTGGCTG ACAAAATCAC CGCTCCCCAC TATCATTGGC 
TCTCCAACTA AGGTAACAGC TCCATTCAAA ATCCCTAAAA CTCAAGCCTC TTGCAATCTC 
ATGGCCCTGG CCGACAGCCC CATCCCCAAC AAGACTCTGA TGGATGGACG TGAACTTTGT 
GCTCATCACT GTACAGCGTA TCCCAGCAGC AGACAGCCCG CACCTGAGGC TAGCAAGACT 
TCCTTTGGCA GGTCTGTAAG CGCTGGCAGA CTGTCTGAGC CTCCGGTACG GATCACTCTA 
GGAGGACAGC CGTACCAGGG CAGCACAGAC AGCCTCAACA CAGAGAGACC CATGGACACA 
GCCCCGGCCG GGATGTGTGC TTTGGCAGCA GGAGGAGGGA GTCCTCGCAC CGTCGTGTTC 
ACGGTGGGTT CACCCCCCAG CAGCAGCACC CCTCCAACCT GCAGCCATCT GGCCTCTCGC 
CCGCGCGCCA CCTCAGTGGG CTCCAACAGC TCTGCCGGTT CGCTCTGCTC CACCAGTGGT 
AAGGTGTACA TGGGCTCTCC TCCAGGCATG ACCATCGGCA GCTCTCCACC CGGAGCAGAA 
GCAGGTCCCA GCAGCCTCCG TTACGTCCCA TACGGCACCT CTCCACCCAG CTTGGATGGC 
TTCATTACCT TTGAAGCTCC AGAACTGCCA GAGGAGACGC TGATGGAGCG TGAGCACACA 
GACACACTGA TGTACCTGCG GATGATGCTG TCTTTCACGG ACTGTGTGCT GGAGATCGCC 
GCTCTGCGGG CCGGAGGGCC TGATCTGGGT GCATCGGCTG CTTCTCTCTA TCCTCCTCAG 
GACAGTGTGG TGGTGGACCA GATCAGTCAG CTCAGTAGAG AGTGGGGGCA GGTGGAGCAG 
CTGGTGTTGT ATATGAAAGC AGCTCAGCTT CTGGCCTCCT CGCTGCACCT CGCCAAAGCC 
CAGATCAAAT CTGCCAAACT CAACCCGTCT ACTGCCGTCA AACAGGTGGT GAAGAGTCTG 
AACGAGCGCT ACAAGAGCTG TATTTCCCTG TGCCGTCGCC TCACAGACAA ACTCAACCAC 
TTCTTCTCTG ACAAGCAGCG CTTTGTGGAC GAGATCAACA GTGTGACAGC AGAAAAGCTC 
ATTTATAACC ACGCTGTGGA GATGGTGCAG TCGGCCGCTC TGGATGAGAT GTTTCAGCAG 
ACTGAGGACA TCGCTTATCG CTACAACAAA GCCTCCATGC TGCTGGAAGG CCTGACCAAG 
ATCCTCCAGG ACCCAGCTGA TATTGAGAAC GTTATTAAAT ACAAGGCCAG TGTGGACCGG 
AGGATCTCAG CTTTGTGTTA CTGCACCGTC ACCCTGTACG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664661.3
CDS	975..4079
Translation	METVGDFEYSRKDLIGHGAFAVVFKGRHKKKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVALYDVQETPSSVFLVMEYCNGGDLADYLQAKGTLREDTLRVFLQQIAAAMRILNSKGIIHRDLKPQNILLSYTGRKKSSINGIRIKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTVIYQCLVGKPPFQANSPQDLRMFYEKNKSLVPNIPRETSPQLEDLLLGLLQRNQKDRIDFDTFFSHPFLEPISTIKKSCPVPVPSCSGLVSDSTCGSSPSCRYVSPPSLPDMQTLPEDVLSSPPLGPPNYLQLSKESGGSTSSKNSSCDTDDFVLVPHLSGEQSYDLPMGAVGRRPSSEFLLCGGSPQPSTGQTPMVSPRSETTPIPVPTQVRNYQRIKQNLSSSPTTTLYGSPRSGTVRRSNTSPMGFPKMVSASPSPADTVQTVGRRLSTGSSRPYSPSPLVGTIPEQLGHCCCGHPQSHEPRSRSSSGGSPVPSSQLLGARLQSAPTLTDIYQSKQKLHKQLSDPVHPTSSAYPSNHSPQLGRPANLGTSPTKHLGSSPRTSDWLTKSPLPTIIGSPTKVTAPFKIPKTQASCNLMALADSPIPNKTLMDGRELCAHHCTAYPSSRQPAPEASKTSFGRSVSAGRLSEPPVRITLGGQPYQGSTDSLNTERPMDTAPAGMCALAAGGGSPRTVVFTVGSPPSSSTPPTCSHLASRPRATSVGSNSSAGSLCSTSGKVYMGSPPGMTIGSSPPGAEAGPSSLRYVPYGTSPPSLDGFITFEAPELPEETLMEREHTDTLMYLRMMLSFTDCVLEIAALRAGGPDLGASAASLYPPQDSVVVDQISQLSREWGQVEQLVLYMKAAQLLASSLHLAKAQIKSAKLNPSTAVKQVVKSLNERYKSCISLCRRLTDKLNHFFSDKQRFVDEINSVTAEKLIYNHAVEMVQSAALDEMFQQTEDIAYRYNKASMLLEGLTKILQDPADIENVIKYKASVDRRISALCYCTVTLYE

Target ORF information:

RefSeq Version	XM_002664615.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 2 (ulk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664615.5

ORF Insert Sequence:

ATGGAGACGG TGGGAGATTT TGAATACAGC CGAAAAGACC TCATCGGACA CGGAGCCTTT 
GCGGTCGTCT TCAAGGGAAG ACATAAAAAG AAGACTGATT GGGAGGTCGC CATCAAAAGC 
ATTAACAAAA AGAATCTCTC CAAATCCCAG ATTCTGCTTG GCAAAGAAAT AAAAATTTTA 
AAGGAACTCC AGCATGAAAA CATTGTGGCG CTCTATGATG TCCAGGAAAC GCCAAGTTCT 
GTATTTCTGG TTATGGAGTA CTGCAATGGA GGAGACCTGG CAGATTATTT GCAAGCCAAG 
GGCACTCTGA GAGAGGACAC ACTGAGGGTC TTTCTACAGC AGATTGCAGC CGCCATGCGA 
ATCCTTAACA GCAAGGGGAT CATCCATCGA GACCTCAAAC CCCAGAACAT CCTGCTGTCC 
TACACGGGCC GCAAGAAGTC CAGCATCAAC GGCATACGCA TCAAGATCGC CGACTTTGGT 
TTTGCGAGAT ACCTCCAGAG CAACATGATG GCAGCCACAC TATGTGGATC CCCGATGTAC 
ATGGCCCCGG AGGTCATCAT GTCCCAGAAT TATGATGCCA AAGCTGACCT GTGGAGCATT 
GGGACTGTTA TTTACCAGTG CCTCGTGGGA AAGCCGCCTT TTCAGGCTAA CAGTCCCCAG 
GACTTGAGGA TGTTCTATGA AAAGAACAAG TCTTTGGTGC CAAACATCCC GAGAGAGACA 
TCTCCTCAAC TGGAAGACCT GCTGCTGGGG CTTCTTCAGA GAAACCAGAA AGATCGTATA 
GATTTTGACA CTTTCTTCAG TCATCCTTTC TTGGAACCGA TATCAACTAT CAAAAAATCA 
TGCCCTGTTC CAGTGCCCTC ATGTTCAGGC TTGGTCTCAG ACAGCACATG TGGCAGCTCT 
CCATCCTGCC GTTACGTGTC TCCTCCGTCA CTGCCAGACA TGCAGACTCT CCCTGAAGAC 
GTGTTGTCCT CTCCACCTCT CGGTCCTCCC AACTATCTGC AGCTGTCCAA GGAGTCCGGC 
GGCAGCACCA GCAGCAAAAA CTCCTCCTGT GACACAGATG ACTTTGTGCT GGTGCCGCAT 
CTGTCTGGGG AACAGTCCTA TGACCTTCCA ATGGGGGCAG TGGGTCGCAG GCCATCCAGT 
GAATTTCTCC TGTGTGGAGG GTCACCGCAG CCATCTACTG GACAGACGCC AATGGTGTCA 
CCACGGAGCG AGACCACGCC AATTCCTGTG CCCACACAGG TGCGAAACTA CCAGCGGATC 
AAACAGAACC TCTCCAGCAG CCCAACCACA ACACTTTATG GCTCTCCCAG GTCAGGCACA 
GTGCGTCGCT CCAACACAAG CCCCATGGGC TTCCCTAAAA TGGTGTCTGC TTCCCCAAGC 
CCTGCAGATA CTGTCCAAAC AGTGGGGCGA CGTCTCTCTA CTGGCAGCTC TCGGCCCTAC 
TCACCCTCTC CACTGGTGGG CACGATTCCT GAGCAGCTCG GCCACTGTTG CTGTGGTCAC 
CCTCAGAGCC ATGAACCGCG CAGTCGCAGC TCTTCAGGCG GTTCCCCAGT GCCATCCTCT 
CAGTTACTGG GCGCTCGTCT TCAGAGTGCG CCGACACTGA CGGACATCTA CCAGAGCAAG 
CAGAAGCTCC ACAAGCAGCT GTCTGACCCA GTGCACCCCA CCTCCTCCGC CTATCCCAGC 
AACCACTCTC CTCAGCTCGG CCGGCCCGCA AACCTCGGCA CCTCCCCAAC CAAACACCTC 
GGTTCCTCTC CTCGCACCTC TGATTGGCTG ACAAAATCAC CGCTCCCCAC TATCATTGGC 
TCTCCAACTA AGGTAACAGC TCCATTCAAA ATCCCTAAAA CTCAAGCCTC TTGCAATCTC 
ATGGCCCTGG CCGACAGCCC CATCCCCAAC AAGACTCTGA TGGATGGACG TGAACTTTGT 
GCTCATCACT GTACAGCGTA TCCCAGCAGC AGACAGCCCG CACCTGAGGC TAGCAAGACT 
TCCTTTGGCA GGTCTGTAAG CGCTGGCAGA CTGTCTGAGC CTCCGGTACG GATCACTCTA 
GGAGGACAGC CGTACCAGGG CAGCACAGAC AGCCTCAACA CAGAGAGACC CATGGACACA 
GCCCCGGCCG GGATGTGTGC TTTGGCAGCA GGAGGAGGGA GTCCTCGCAC CGTCGTGTTC 
ACGGTGGGTT CACCCCCCAG CAGCAGCACC CCTCCAACCT GCAGCCATCT GGCCTCTCGC 
CCGCGCGCCA CCTCAGTGGG CTCCAACAGC TCTGCCGGTT CGCTCTGCTC CACCAGTGGT 
AAGGTGTACA TGGGCTCTCC TCCAGGCATG ACCATCGGCA GCTCTCCACC CGGAGCAGAA 
GCAGGTCCCA GCAGCCTCCG TTACGTCCCA TACGGCACCT CTCCACCCAG CTTGGATGGC 
TTCATTACCT TTGAAGCTCC AGAACTGCCA GAGGAGACGC TGATGGAGCG TGAGCACACA 
GACACACTGA TGTACCTGCG GATGATGCTG TCTTTCACGG ACTGTGTGCT GGAGATCGCC 
GCTCTGCGGG CCGGAGGGCC TGATCTGGGT GCATCGGCTG CTTCTCTCTA TCCTCCTCAG 
GACAGTGTGG TGGTGGACCA GATCAGTCAG CTCAGTAGAG AGTGGGGGCA GGTGGAGCAG 
CTGGTGTTGT ATATGAAAGC AGCTCAGCTT CTGGCCTCCT CGCTGCACCT CGCCAAAGCC 
CAGATCAAAT CTGCCAAACT CAACCCGTCT ACTGCCGTCA AACAGGTGGT GAAGAGTCTG 
AACGAGCGCT ACAAGAGCTG TATTTCCCTG TGCCGTCGCC TCACAGACAA ACTCAACCAC 
TTCTTCTCTG ACAAGCAGCG CTTTGTGGAC GAGATCAACA GTGTGACAGC AGAAAAGCTC 
ATTTATAACC ACGCTGTGGA GATGGTGCAG TCGGCCGCTC TGGATGAGAT GTTTCAGCAG 
ACTGAGGACA TCGCTTATCG CTACAACAAA GCCTCCATGC TGCTGGAAGG CCTGACCAAG 
ATCCTCCAGG ACCCAGCTGA TATTGAGAAC GTTATTAAAT ACAAGGCCAG TGTGGACCGG 
AGGATCTCAG CTTTGTGTTA CTGCACCGTC ACCCTGTACG AATAA