lrfn5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrfn5a
Entrez Gene ID	100144400
Full Name	leucine rich repeat and fibronectin type III domain containing 5a
Synonyms	sc:d0343,si:ch73-206h2.1
General protein information	Preferred Names leucine rich repeat and fibronectin type III domain containing 5a Names leucine-rich repeat and fibronectin type-III domain-containing protein 5 leucine rich repeat and fibronectin type III domain containing 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001921639.6	XP_001921674.1	leucine-rich repeat and fibronectin type-III domain-containing protein 5
XM_001921639.7	XP_001921674.1	leucine-rich repeat and fibronectin type-III domain-containing protein 5

Pathways from BioSystems

Homologies

	LRFN5	XP_001094064.1
Rattus norvegicus (Norway rat)	Lrfn5	NP_001101494.1
Gallus gallus (chicken)	LRFN5	XP_004936591.1
Homo sapiens (human)	LRFN5	NP_689660.2
Pan troglodytes (chimpanzee)	LRFN5	XP_001149289.1
Canis lupus familiaris (dog)	LOC490666	XP_005623411.1
Danio rerio (zebrafish)	si:ch73-206h2.1	XP_001921674.1
Xenopus tropicalis (tropical clawed frog)	lrfn5	XP_002941802.1
Mus musculus (house mouse)	Lrfn5	NP_848829.2

Gene Ontology

The following lrfn5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrfn5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22316	XM_001921639.7 Latest version!	Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa22316	XM_001921639.6	Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrfn5a ( XM_001921639.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921674.1 Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.

Price & Availability↑

CloneID	ODa22316
Clone ID Related Accession (Same CDS sequence)	XM_001921639.6 , XM_001921639.7
Accession Version	XM_001921639.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat and fibronectin type-III domain-containing protein 5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921639.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAC TGCTCGTCTG CTTGTTGGTT ATCGGAATGG CAGTGAAGGC CCAGATCTGT 
CCAAAGCGCT GCGTCTGTCA GGTTCTTTCC CCCAACCTCG CAACACTCTG TGCCAAAAAA 
GGGCTGCTCT TCGTCCCCCC TAACATCGAC AGGCACACCG TGGAGCTCCG GCTAGCTGAT 
AATTTTGTCA CCAGCGTCAA ACGGAAAGAC TTGGCCAACA TGACCAAGCT GGTGGATCTT 
ACGTTATCCA GGAATACAAT AAGCTACATC ACTCCACATG CATTCGCAGA CCTGGAAAAC 
CTACGAGCCT TGCATCTAGA CCACAACCGC TTGACGCGAA TAGCCAACGA CACTTTCAGT 
GGCATGTCCA AGCTGCATCA TCTAATCCTG AACAACAACC AACTGACCCT CATCCACATG 
GGAGCCTTTA ATGACCTACT AGCCCTCGAA GAGCTGGATT TATCCTACAA CAACCTCGAG 
ACCATCCCCT GGGAGGCCAT TCAAAGGATG ACCAGCCTAC ACACTCTCAG TCTGGACCAC 
AATATGATTG AATACATTCC TGAGGGGACG TTCTCCCTCC TACAGAAGCT CAATCGCTTG 
GACGTGACCT CCAACAAGCT CCAGAAGCTC CCACCGGACC CTCTTTTCCA ACGAGCACAG 
GTTCTGGCAA CATCCGGCAT TATGAATCCC TCTTCTTTTG CGCTAAGCTT CGGAGGCAAT 
CCTTTACATT GCAACTGCGA GCTACTGTGG CTTCGGCGGC TCAATCGCGA GGACGACTTG 
GAGACCTGCG CCACACCTTT GCACCTCTCT GGACGCTATT TCTGGTCCAT CCCCGAGGAG 
GAGTTCCTCT GTGAGCCGCC CCTCATTACC AGGCACTCTC ACGAAATGAG GGTTCTGGAG 
GGCCAACGAG TGGCATTAAA GTGCAAAGCC AGGGGAGATC CCGAACCTGC CATACATTGG 
ATATCTCCCG AGGGAAAGTT AGTCTCGAAC ACGTCACGCA CGCTGGTTTA CAACAACGGC 
ACGCTGGACA TCCTGATAAG CACAGTAAAA GACACAGGCT CTTTCACCTG CATCTCCTCC 
AATCCTGCAG GGGAGGCTCA TCAGACCATA GAGCTGGTCA TCATTAAGCT TCCACACATC 
AGCAACAGCA CAAATAACAT CCAGGAGCCG GACCCGGGCT CTTCTGATAT CTCCACTTCT 
ACAAGGGCAG GAGCAAACGG TGGCAACCAC ACCGGTGACA CCAAGACCAG CTCCGATAAG 
AGAGTGGTTA TGGCAGAAGC CACTTCCTCC ACGGCACTCA TCAAGTTCAA CTTTCAGAGG 
AATATACCTG GAATACGCAT GTTTCAGATT CAGTACAATG GAACCTACGA TGACTCTCTT 
GTTTACAGAA TGATTCCCCC AACGAGTAAA AACTTCCTTG TGAATAACCT GGCGGCCGGG 
ACACAGTACG ACCTTTGTGT GCTGGCCATT TATGATGATG GCATGACCTC CCTGACGGCC 
ACGCGGGTTG TGGGCTGCGT CCAGTTCACC ACAGAGTCTG AATATCTGCG CTGCCACTTC 
ATGCAGTCCC AGTTCTTAGG CGGCACTATG ATCATCATCA TCGGTGGCAT AATCGTGGCC 
TCCGTCCTCG TCTTCATCAT CATCCTCATG ATCCGCTATA AAGTGTGCAA CAGCGGCGAC 
GCAGCAAAGG GAACGTCAGT CATCGATGTG CACTCACAAA CCAACGGTGC GCAAGGATGT 
ACCGTCACTC CGTCTGCATC CAAACAAGGT GCTTTGAGCT TGGAGGAGGG TGAGGGGGGC 
AAAAAGAGCT CCGCCGCACC TCTTCCTCCA CCTCCGGATT CCCAAACGCA CACCTCAGAG 
ACCTCCATCC CAGACTGCTC CACCTCCACC TCCACGGTAA GTCAAAGCTG GGCGACTCCC 
GGCTCTTCAG GGTCACTAAA GCCAAAGCGC AAACCAGCGC CAAAGCCCGT CCCGACAGCT 
GAGCAGCCCA AAGCAGAAGT GCTGCCCAAT GCAGAGACGC AAAATACCAA TCGCAACAAC 
TCCACCGCCC TTTACCAACC TGCCCCACTC CCTCCACCCC CGTCCCGCTT CAAAGACACC 
CCCATTTTAA GAAGGGCGCG TCCGTCTACG TCCAAGTATA TGACTCTGCC CGTTGAAGGA 
GCGTGGGCCA AACGCAGGTA CTCTTTGAAC GAGAACCTGT CAAAACATCA CTGCTACATT 
GGCTCGCAGA AGCTGGGAAA TGTGTTTTGC AAGCGGAGTA TGTCCATGAA CGGAATGCTT 
GTCCAAATGG CAAACTCTAA TCTAGACAGT GAAAAAGCGG CATTTTCCAG TTCCGAGTGG 
ATTCTTGAGA GCACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921674.1
CDS	836..3196
Translation	MEKLLVCLLVIGMAVKAQICPKRCVCQVLSPNLATLCAKKGLLFVPPNIDRHTVELRLADNFVTSVKRKDLANMTKLVDLTLSRNTISYITPHAFADLENLRALHLDHNRLTRIANDTFSGMSKLHHLILNNNQLTLIHMGAFNDLLALEELDLSYNNLETIPWEAIQRMTSLHTLSLDHNMIEYIPEGTFSLLQKLNRLDVTSNKLQKLPPDPLFQRAQVLATSGIMNPSSFALSFGGNPLHCNCELLWLRRLNREDDLETCATPLHLSGRYFWSIPEEEFLCEPPLITRHSHEMRVLEGQRVALKCKARGDPEPAIHWISPEGKLVSNTSRTLVYNNGTLDILISTVKDTGSFTCISSNPAGEAHQTIELVIIKLPHISNSTNNIQEPDPGSSDISTSTRAGANGGNHTGDTKTSSDKRVVMAEATSSTALIKFNFQRNIPGIRMFQIQYNGTYDDSLVYRMIPPTSKNFLVNNLAAGTQYDLCVLAIYDDGMTSLTATRVVGCVQFTTESEYLRCHFMQSQFLGGTMIIIIGGIIVASVLVFIIILMIRYKVCNSGDAAKGTSVIDVHSQTNGAQGCTVTPSASKQGALSLEEGEGGKKSSAAPLPPPPDSQTHTSETSIPDCSTSTSTVSQSWATPGSSGSLKPKRKPAPKPVPTAEQPKAEVLPNAETQNTNRNNSTALYQPAPLPPPPSRFKDTPILRRARPSTSKYMTLPVEGAWAKRRYSLNENLSKHHCYIGSQKLGNVFCKRSMSMNGMLVQMANSNLDSEKAAFSSSEWILESTV

Target ORF information:

RefSeq Version	XM_001921639.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921639.6

ORF Insert Sequence:

ATGGAAAAAC TGCTCGTCTG CTTGTTGGTT ATCGGAATGG CAGTGAAGGC CCAGATCTGT 
CCAAAGCGCT GCGTCTGTCA GGTTCTTTCC CCCAACCTCG CAACACTCTG TGCCAAAAAA 
GGGCTGCTCT TCGTCCCCCC TAACATCGAC AGGCACACCG TGGAGCTCCG GCTAGCTGAT 
AATTTTGTCA CCAGCGTCAA ACGGAAAGAC TTGGCCAACA TGACCAAGCT GGTGGATCTT 
ACGTTATCCA GGAATACAAT AAGCTACATC ACTCCACATG CATTCGCAGA CCTGGAAAAC 
CTACGAGCCT TGCATCTAGA CCACAACCGC TTGACGCGAA TAGCCAACGA CACTTTCAGT 
GGCATGTCCA AGCTGCATCA TCTAATCCTG AACAACAACC AACTGACCCT CATCCACATG 
GGAGCCTTTA ATGACCTACT AGCCCTCGAA GAGCTGGATT TATCCTACAA CAACCTCGAG 
ACCATCCCCT GGGAGGCCAT TCAAAGGATG ACCAGCCTAC ACACTCTCAG TCTGGACCAC 
AATATGATTG AATACATTCC TGAGGGGACG TTCTCCCTCC TACAGAAGCT CAATCGCTTG 
GACGTGACCT CCAACAAGCT CCAGAAGCTC CCACCGGACC CTCTTTTCCA ACGAGCACAG 
GTTCTGGCAA CATCCGGCAT TATGAATCCC TCTTCTTTTG CGCTAAGCTT CGGAGGCAAT 
CCTTTACATT GCAACTGCGA GCTACTGTGG CTTCGGCGGC TCAATCGCGA GGACGACTTG 
GAGACCTGCG CCACACCTTT GCACCTCTCT GGACGCTATT TCTGGTCCAT CCCCGAGGAG 
GAGTTCCTCT GTGAGCCGCC CCTCATTACC AGGCACTCTC ACGAAATGAG GGTTCTGGAG 
GGCCAACGAG TGGCATTAAA GTGCAAAGCC AGGGGAGATC CCGAACCTGC CATACATTGG 
ATATCTCCCG AGGGAAAGTT AGTCTCGAAC ACGTCACGCA CGCTGGTTTA CAACAACGGC 
ACGCTGGACA TCCTGATAAG CACAGTAAAA GACACAGGCT CTTTCACCTG CATCTCCTCC 
AATCCTGCAG GGGAGGCTCA TCAGACCATA GAGCTGGTCA TCATTAAGCT TCCACACATC 
AGCAACAGCA CAAATAACAT CCAGGAGCCG GACCCGGGCT CTTCTGATAT CTCCACTTCT 
ACAAGGGCAG GAGCAAACGG TGGCAACCAC ACCGGTGACA CCAAGACCAG CTCCGATAAG 
AGAGTGGTTA TGGCAGAAGC CACTTCCTCC ACGGCACTCA TCAAGTTCAA CTTTCAGAGG 
AATATACCTG GAATACGCAT GTTTCAGATT CAGTACAATG GAACCTACGA TGACTCTCTT 
GTTTACAGAA TGATTCCCCC AACGAGTAAA AACTTCCTTG TGAATAACCT GGCGGCCGGG 
ACACAGTACG ACCTTTGTGT GCTGGCCATT TATGATGATG GCATGACCTC CCTGACGGCC 
ACGCGGGTTG TGGGCTGCGT CCAGTTCACC ACAGAGTCTG AATATCTGCG CTGCCACTTC 
ATGCAGTCCC AGTTCTTAGG CGGCACTATG ATCATCATCA TCGGTGGCAT AATCGTGGCC 
TCCGTCCTCG TCTTCATCAT CATCCTCATG ATCCGCTATA AAGTGTGCAA CAGCGGCGAC 
GCAGCAAAGG GAACGTCAGT CATCGATGTG CACTCACAAA CCAACGGTGC GCAAGGATGT 
ACCGTCACTC CGTCTGCATC CAAACAAGGT GCTTTGAGCT TGGAGGAGGG TGAGGGGGGC 
AAAAAGAGCT CCGCCGCACC TCTTCCTCCA CCTCCGGATT CCCAAACGCA CACCTCAGAG 
ACCTCCATCC CAGACTGCTC CACCTCCACC TCCACGGTAA GTCAAAGCTG GGCGACTCCC 
GGCTCTTCAG GGTCACTAAA GCCAAAGCGC AAACCAGCGC CAAAGCCCGT CCCGACAGCT 
GAGCAGCCCA AAGCAGAAGT GCTGCCCAAT GCAGAGACGC AAAATACCAA TCGCAACAAC 
TCCACCGCCC TTTACCAACC TGCCCCACTC CCTCCACCCC CGTCCCGCTT CAAAGACACC 
CCCATTTTAA GAAGGGCGCG TCCGTCTACG TCCAAGTATA TGACTCTGCC CGTTGAAGGA 
GCGTGGGCCA AACGCAGGTA CTCTTTGAAC GAGAACCTGT CAAAACATCA CTGCTACATT 
GGCTCGCAGA AGCTGGGAAA TGTGTTTTGC AAGCGGAGTA TGTCCATGAA CGGAATGCTT 
GTCCAAATGG CAAACTCTAA TCTAGACAGT GAAAAAGCGG CATTTTCCAG TTCCGAGTGG 
ATTCTTGAGA GCACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrfn5a ( XM_001921639.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921674.1 Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.

Price & Availability↑

CloneID	ODa22316
Clone ID Related Accession (Same CDS sequence)	XM_001921639.6 , XM_001921639.7
Accession Version	XM_001921639.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat and fibronectin type-III domain-containing protein 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921639.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAC TGCTCGTCTG CTTGTTGGTT ATCGGAATGG CAGTGAAGGC CCAGATCTGT 
CCAAAGCGCT GCGTCTGTCA GGTTCTTTCC CCCAACCTCG CAACACTCTG TGCCAAAAAA 
GGGCTGCTCT TCGTCCCCCC TAACATCGAC AGGCACACCG TGGAGCTCCG GCTAGCTGAT 
AATTTTGTCA CCAGCGTCAA ACGGAAAGAC TTGGCCAACA TGACCAAGCT GGTGGATCTT 
ACGTTATCCA GGAATACAAT AAGCTACATC ACTCCACATG CATTCGCAGA CCTGGAAAAC 
CTACGAGCCT TGCATCTAGA CCACAACCGC TTGACGCGAA TAGCCAACGA CACTTTCAGT 
GGCATGTCCA AGCTGCATCA TCTAATCCTG AACAACAACC AACTGACCCT CATCCACATG 
GGAGCCTTTA ATGACCTACT AGCCCTCGAA GAGCTGGATT TATCCTACAA CAACCTCGAG 
ACCATCCCCT GGGAGGCCAT TCAAAGGATG ACCAGCCTAC ACACTCTCAG TCTGGACCAC 
AATATGATTG AATACATTCC TGAGGGGACG TTCTCCCTCC TACAGAAGCT CAATCGCTTG 
GACGTGACCT CCAACAAGCT CCAGAAGCTC CCACCGGACC CTCTTTTCCA ACGAGCACAG 
GTTCTGGCAA CATCCGGCAT TATGAATCCC TCTTCTTTTG CGCTAAGCTT CGGAGGCAAT 
CCTTTACATT GCAACTGCGA GCTACTGTGG CTTCGGCGGC TCAATCGCGA GGACGACTTG 
GAGACCTGCG CCACACCTTT GCACCTCTCT GGACGCTATT TCTGGTCCAT CCCCGAGGAG 
GAGTTCCTCT GTGAGCCGCC CCTCATTACC AGGCACTCTC ACGAAATGAG GGTTCTGGAG 
GGCCAACGAG TGGCATTAAA GTGCAAAGCC AGGGGAGATC CCGAACCTGC CATACATTGG 
ATATCTCCCG AGGGAAAGTT AGTCTCGAAC ACGTCACGCA CGCTGGTTTA CAACAACGGC 
ACGCTGGACA TCCTGATAAG CACAGTAAAA GACACAGGCT CTTTCACCTG CATCTCCTCC 
AATCCTGCAG GGGAGGCTCA TCAGACCATA GAGCTGGTCA TCATTAAGCT TCCACACATC 
AGCAACAGCA CAAATAACAT CCAGGAGCCG GACCCGGGCT CTTCTGATAT CTCCACTTCT 
ACAAGGGCAG GAGCAAACGG TGGCAACCAC ACCGGTGACA CCAAGACCAG CTCCGATAAG 
AGAGTGGTTA TGGCAGAAGC CACTTCCTCC ACGGCACTCA TCAAGTTCAA CTTTCAGAGG 
AATATACCTG GAATACGCAT GTTTCAGATT CAGTACAATG GAACCTACGA TGACTCTCTT 
GTTTACAGAA TGATTCCCCC AACGAGTAAA AACTTCCTTG TGAATAACCT GGCGGCCGGG 
ACACAGTACG ACCTTTGTGT GCTGGCCATT TATGATGATG GCATGACCTC CCTGACGGCC 
ACGCGGGTTG TGGGCTGCGT CCAGTTCACC ACAGAGTCTG AATATCTGCG CTGCCACTTC 
ATGCAGTCCC AGTTCTTAGG CGGCACTATG ATCATCATCA TCGGTGGCAT AATCGTGGCC 
TCCGTCCTCG TCTTCATCAT CATCCTCATG ATCCGCTATA AAGTGTGCAA CAGCGGCGAC 
GCAGCAAAGG GAACGTCAGT CATCGATGTG CACTCACAAA CCAACGGTGC GCAAGGATGT 
ACCGTCACTC CGTCTGCATC CAAACAAGGT GCTTTGAGCT TGGAGGAGGG TGAGGGGGGC 
AAAAAGAGCT CCGCCGCACC TCTTCCTCCA CCTCCGGATT CCCAAACGCA CACCTCAGAG 
ACCTCCATCC CAGACTGCTC CACCTCCACC TCCACGGTAA GTCAAAGCTG GGCGACTCCC 
GGCTCTTCAG GGTCACTAAA GCCAAAGCGC AAACCAGCGC CAAAGCCCGT CCCGACAGCT 
GAGCAGCCCA AAGCAGAAGT GCTGCCCAAT GCAGAGACGC AAAATACCAA TCGCAACAAC 
TCCACCGCCC TTTACCAACC TGCCCCACTC CCTCCACCCC CGTCCCGCTT CAAAGACACC 
CCCATTTTAA GAAGGGCGCG TCCGTCTACG TCCAAGTATA TGACTCTGCC CGTTGAAGGA 
GCGTGGGCCA AACGCAGGTA CTCTTTGAAC GAGAACCTGT CAAAACATCA CTGCTACATT 
GGCTCGCAGA AGCTGGGAAA TGTGTTTTGC AAGCGGAGTA TGTCCATGAA CGGAATGCTT 
GTCCAAATGG CAAACTCTAA TCTAGACAGT GAAAAAGCGG CATTTTCCAG TTCCGAGTGG 
ATTCTTGAGA GCACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921674.1
CDS	888..3248
Translation	MEKLLVCLLVIGMAVKAQICPKRCVCQVLSPNLATLCAKKGLLFVPPNIDRHTVELRLADNFVTSVKRKDLANMTKLVDLTLSRNTISYITPHAFADLENLRALHLDHNRLTRIANDTFSGMSKLHHLILNNNQLTLIHMGAFNDLLALEELDLSYNNLETIPWEAIQRMTSLHTLSLDHNMIEYIPEGTFSLLQKLNRLDVTSNKLQKLPPDPLFQRAQVLATSGIMNPSSFALSFGGNPLHCNCELLWLRRLNREDDLETCATPLHLSGRYFWSIPEEEFLCEPPLITRHSHEMRVLEGQRVALKCKARGDPEPAIHWISPEGKLVSNTSRTLVYNNGTLDILISTVKDTGSFTCISSNPAGEAHQTIELVIIKLPHISNSTNNIQEPDPGSSDISTSTRAGANGGNHTGDTKTSSDKRVVMAEATSSTALIKFNFQRNIPGIRMFQIQYNGTYDDSLVYRMIPPTSKNFLVNNLAAGTQYDLCVLAIYDDGMTSLTATRVVGCVQFTTESEYLRCHFMQSQFLGGTMIIIIGGIIVASVLVFIIILMIRYKVCNSGDAAKGTSVIDVHSQTNGAQGCTVTPSASKQGALSLEEGEGGKKSSAAPLPPPPDSQTHTSETSIPDCSTSTSTVSQSWATPGSSGSLKPKRKPAPKPVPTAEQPKAEVLPNAETQNTNRNNSTALYQPAPLPPPPSRFKDTPILRRARPSTSKYMTLPVEGAWAKRRYSLNENLSKHHCYIGSQKLGNVFCKRSMSMNGMLVQMANSNLDSEKAAFSSSEWILESTV

Target ORF information:

RefSeq Version	XM_001921639.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine rich repeat and fibronectin type III domain containing 5a (lrfn5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921639.7

ORF Insert Sequence:

ATGGAAAAAC TGCTCGTCTG CTTGTTGGTT ATCGGAATGG CAGTGAAGGC CCAGATCTGT 
CCAAAGCGCT GCGTCTGTCA GGTTCTTTCC CCCAACCTCG CAACACTCTG TGCCAAAAAA 
GGGCTGCTCT TCGTCCCCCC TAACATCGAC AGGCACACCG TGGAGCTCCG GCTAGCTGAT 
AATTTTGTCA CCAGCGTCAA ACGGAAAGAC TTGGCCAACA TGACCAAGCT GGTGGATCTT 
ACGTTATCCA GGAATACAAT AAGCTACATC ACTCCACATG CATTCGCAGA CCTGGAAAAC 
CTACGAGCCT TGCATCTAGA CCACAACCGC TTGACGCGAA TAGCCAACGA CACTTTCAGT 
GGCATGTCCA AGCTGCATCA TCTAATCCTG AACAACAACC AACTGACCCT CATCCACATG 
GGAGCCTTTA ATGACCTACT AGCCCTCGAA GAGCTGGATT TATCCTACAA CAACCTCGAG 
ACCATCCCCT GGGAGGCCAT TCAAAGGATG ACCAGCCTAC ACACTCTCAG TCTGGACCAC 
AATATGATTG AATACATTCC TGAGGGGACG TTCTCCCTCC TACAGAAGCT CAATCGCTTG 
GACGTGACCT CCAACAAGCT CCAGAAGCTC CCACCGGACC CTCTTTTCCA ACGAGCACAG 
GTTCTGGCAA CATCCGGCAT TATGAATCCC TCTTCTTTTG CGCTAAGCTT CGGAGGCAAT 
CCTTTACATT GCAACTGCGA GCTACTGTGG CTTCGGCGGC TCAATCGCGA GGACGACTTG 
GAGACCTGCG CCACACCTTT GCACCTCTCT GGACGCTATT TCTGGTCCAT CCCCGAGGAG 
GAGTTCCTCT GTGAGCCGCC CCTCATTACC AGGCACTCTC ACGAAATGAG GGTTCTGGAG 
GGCCAACGAG TGGCATTAAA GTGCAAAGCC AGGGGAGATC CCGAACCTGC CATACATTGG 
ATATCTCCCG AGGGAAAGTT AGTCTCGAAC ACGTCACGCA CGCTGGTTTA CAACAACGGC 
ACGCTGGACA TCCTGATAAG CACAGTAAAA GACACAGGCT CTTTCACCTG CATCTCCTCC 
AATCCTGCAG GGGAGGCTCA TCAGACCATA GAGCTGGTCA TCATTAAGCT TCCACACATC 
AGCAACAGCA CAAATAACAT CCAGGAGCCG GACCCGGGCT CTTCTGATAT CTCCACTTCT 
ACAAGGGCAG GAGCAAACGG TGGCAACCAC ACCGGTGACA CCAAGACCAG CTCCGATAAG 
AGAGTGGTTA TGGCAGAAGC CACTTCCTCC ACGGCACTCA TCAAGTTCAA CTTTCAGAGG 
AATATACCTG GAATACGCAT GTTTCAGATT CAGTACAATG GAACCTACGA TGACTCTCTT 
GTTTACAGAA TGATTCCCCC AACGAGTAAA AACTTCCTTG TGAATAACCT GGCGGCCGGG 
ACACAGTACG ACCTTTGTGT GCTGGCCATT TATGATGATG GCATGACCTC CCTGACGGCC 
ACGCGGGTTG TGGGCTGCGT CCAGTTCACC ACAGAGTCTG AATATCTGCG CTGCCACTTC 
ATGCAGTCCC AGTTCTTAGG CGGCACTATG ATCATCATCA TCGGTGGCAT AATCGTGGCC 
TCCGTCCTCG TCTTCATCAT CATCCTCATG ATCCGCTATA AAGTGTGCAA CAGCGGCGAC 
GCAGCAAAGG GAACGTCAGT CATCGATGTG CACTCACAAA CCAACGGTGC GCAAGGATGT 
ACCGTCACTC CGTCTGCATC CAAACAAGGT GCTTTGAGCT TGGAGGAGGG TGAGGGGGGC 
AAAAAGAGCT CCGCCGCACC TCTTCCTCCA CCTCCGGATT CCCAAACGCA CACCTCAGAG 
ACCTCCATCC CAGACTGCTC CACCTCCACC TCCACGGTAA GTCAAAGCTG GGCGACTCCC 
GGCTCTTCAG GGTCACTAAA GCCAAAGCGC AAACCAGCGC CAAAGCCCGT CCCGACAGCT 
GAGCAGCCCA AAGCAGAAGT GCTGCCCAAT GCAGAGACGC AAAATACCAA TCGCAACAAC 
TCCACCGCCC TTTACCAACC TGCCCCACTC CCTCCACCCC CGTCCCGCTT CAAAGACACC 
CCCATTTTAA GAAGGGCGCG TCCGTCTACG TCCAAGTATA TGACTCTGCC CGTTGAAGGA 
GCGTGGGCCA AACGCAGGTA CTCTTTGAAC GAGAACCTGT CAAAACATCA CTGCTACATT 
GGCTCGCAGA AGCTGGGAAA TGTGTTTTGC AAGCGGAGTA TGTCCATGAA CGGAATGCTT 
GTCCAAATGG CAAACTCTAA TCTAGACAGT GAAAAAGCGG CATTTTCCAG TTCCGAGTGG 
ATTCTTGAGA GCACAGTGTG A