LOC107091395 gene cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

mRNA	Protein	Name
XM_015385271.1	XP_015240757.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
XM_015385279.1	XP_015240765.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X11
XM_015385269.1	XP_015240755.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
XM_015385270.1	XP_015240756.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
XM_015385273.1	XP_015240759.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
XM_015385272.1	XP_015240758.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
XM_015385268.1	XP_015240754.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
XM_015385274.1	XP_015240760.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
XM_015385275.1	XP_015240761.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
XM_015385277.1	XP_015240763.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
XM_015385278.1	XP_015240764.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
XM_015385276.1	XP_015240762.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
XM_015385281.1	XP_015240767.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy15965	XM_015385278.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy15966	XM_015385279.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy15967	XM_015385281.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy15956	XM_015385268.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCy15957	XM_015385269.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCy15958	XM_015385270.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCy15959	XM_015385271.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCy15960	XM_015385272.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCy15961	XM_015385274.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCy15961	XM_015385273.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCy15962	XM_015385275.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCy15963	XM_015385276.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCy15964	XM_015385277.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

LOC107091395 ( XM_015385278.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240764.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCy15965
Clone ID Related Accession (Same CDS sequence)	XM_015385278.1
Accession Version	XM_015385278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GCTGGAAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGCC GCATCATCGA GGGGAGTCCA GCGGACCGCT GCGGGAAGCT GAAGGTGGGC 
GACCGCATCC TGGCTGTCAA CGGGAACTCC ATCACCAACA AGTCCCACTC TGACATCGTC 
AACCTGATCA AGGAGGCTGG GAACACCGTC ACGCTGCGGA TCATTCCTGG AGATGAGTCA 
TCCAATGCAT CTTTGTTGAC AAATGCAGAG AAGATTGCCA CCATAACCAC CACTCACACA 
GCTCAGCAAC AGGCAGCACC TGAGGCCAGG ACCAACACTA AACCAAAACA GGAATCTTAT 
GAGTTTAAAG CTCCTACGAA CACTGCTCCC CAGCCCCCCA TCCAGCAAGA CTCAGCTCAG 
GACTCCGAGT TCTACTCTGT GGACCTGGAG AGGGACAACA AAGGCTTTGG CTTCAGCCTG 
CGGGGAGGAA GAGAGTACAA CATGGACCTG TACGTGCTGA GGCTTGCTGA AGAAGGAGCT 
GCTGTTCGCA ATGGGAAAAT GAGGGTTGGA GATGAGATCT TGGAGATCAA CGGCGAAAGC 
ACCAAAGGCA TGAAGCACGC ACGGGCCATC GAGCTCATCA AGAACGGAGG CCGAAACGTC 
CACCTGGTGC TCAAGAGGGG TGACGGCTCC GTGCCTGAAT ATGGGATGGC CGCTCCCCAT 
CTCACAGCAA GTGTGAGAAA TGACAAGATG GGGGAGGCCT CTGTCCTCCA AAATCCGACC 
ACGACGGCAG CCCCAACGAC ATCAGCACAG TTGACCCAGT CACAAGGATA CAAAACGCTG 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240764.1
CDS	388..4476
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYGMAAPHLTASVRNDKMGEASVLQNPTTTAAPTTSAQLTQSQGYKTLRK

Target ORF information:

RefSeq Version	XM_015385278.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385278.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GCTGGAAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGCC GCATCATCGA GGGGAGTCCA GCGGACCGCT GCGGGAAGCT GAAGGTGGGC 
GACCGCATCC TGGCTGTCAA CGGGAACTCC ATCACCAACA AGTCCCACTC TGACATCGTC 
AACCTGATCA AGGAGGCTGG GAACACCGTC ACGCTGCGGA TCATTCCTGG AGATGAGTCA 
TCCAATGCAT CTTTGTTGAC AAATGCAGAG AAGATTGCCA CCATAACCAC CACTCACACA 
GCTCAGCAAC AGGCAGCACC TGAGGCCAGG ACCAACACTA AACCAAAACA GGAATCTTAT 
GAGTTTAAAG CTCCTACGAA CACTGCTCCC CAGCCCCCCA TCCAGCAAGA CTCAGCTCAG 
GACTCCGAGT TCTACTCTGT GGACCTGGAG AGGGACAACA AAGGCTTTGG CTTCAGCCTG 
CGGGGAGGAA GAGAGTACAA CATGGACCTG TACGTGCTGA GGCTTGCTGA AGAAGGAGCT 
GCTGTTCGCA ATGGGAAAAT GAGGGTTGGA GATGAGATCT TGGAGATCAA CGGCGAAAGC 
ACCAAAGGCA TGAAGCACGC ACGGGCCATC GAGCTCATCA AGAACGGAGG CCGAAACGTC 
CACCTGGTGC TCAAGAGGGG TGACGGCTCC GTGCCTGAAT ATGGGATGGC CGCTCCCCAT 
CTCACAGCAA GTGTGAGAAA TGACAAGATG GGGGAGGCCT CTGTCCTCCA AAATCCGACC 
ACGACGGCAG CCCCAACGAC ATCAGCACAG TTGACCCAGT CACAAGGATA CAAAACGCTG 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385279.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240765.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCy15966
Clone ID Related Accession (Same CDS sequence)	XM_015385279.1
Accession Version	XM_015385279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGGG 
ATGGCCGCTC CCCATCTCAC AGCAAGTGTG AGAAATGACA AGATGGGGGA GGCCTCTGTC 
CTCCAAAATC CGACCACGAC GGCAGCCCCA ACGACATCAG CACAGTTGAC CCAGTCACAA 
GGATACAAAA CGCTGCGGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240765.1
CDS	388..4431
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYGMAAPHLTASVRNDKMGEASVLQNPTTTAAPTTSAQLTQSQGYKTLRK

Target ORF information:

RefSeq Version	XM_015385279.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385279.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGGG 
ATGGCCGCTC CCCATCTCAC AGCAAGTGTG AGAAATGACA AGATGGGGGA GGCCTCTGTC 
CTCCAAAATC CGACCACGAC GGCAGCCCCA ACGACATCAG CACAGTTGAC CCAGTCACAA 
GGATACAAAA CGCTGCGGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385281.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240767.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X13, mRNA.

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CloneID	OCy15967
Clone ID Related Accession (Same CDS sequence)	XM_015385281.1
Accession Version	XM_015385281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGGT 
GGGTCAATCT ACGAAAACAT TCCCTTCTCC CCCATCTTCA CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240767.1
CDS	388..4335
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYGGSIYENIPFSPIFTP

Target ORF information:

RefSeq Version	XM_015385281.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385281.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGGT 
GGGTCAATCT ACGAAAACAT TCCCTTCTCC CCCATCTTCA CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385268.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240754.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X1, mRNA.

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CloneID	OCy15956
Clone ID Related Accession (Same CDS sequence)	XM_015385268.1
Accession Version	XM_015385268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GCTGGAAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGCC GCATCATCGA GGGGAGTCCA GCGGACCGCT GCGGGAAGCT GAAGGTGGGC 
GACCGCATCC TGGCTGTCAA CGGGAACTCC ATCACCAACA AGTCCCACTC TGACATCGTC 
AACCTGATCA AGGAGGCTGG GAACACCGTC ACGCTGCGGA TCATTCCTGG AGATGAGTCA 
TCCAATGCAT CTTTGTTGAC AAATGCAGAG AAGATTGCCA CCATAACCAC CACTCACACA 
GCTCAGCAAC AGGCAGCACC TGAGGCCAGG ACCAACACTA AACCAAAACA GGAATCTTAT 
GAGTTTAAAG CTCCTACGAA CACTGCTCCC CAGCCCCCCA TCCAGCAAGA CTCAGCTCAG 
GACTCCGAGT TCTACTCTGT GGACCTGGAG AGGGACAACA AAGGCTTTGG CTTCAGCCTG 
CGGGGAGGAA GAGAGTACAA CATGGACCTG TACGTGCTGA GGCTTGCTGA AGAAGGAGCT 
GCTGTTCGCA ATGGGAAAAT GAGGGTTGGA GATGAGATCT TGGAGATCAA CGGCGAAAGC 
ACCAAAGGCA TGAAGCACGC ACGGGCCATC GAGCTCATCA AGAACGGAGG CCGAAACGTC 
CACCTGGTGC TCAAGAGGGG TGACGGCTCC GTGCCTGAAT ATGACGGCAG CCCCAACGAC 
ATCAGCACAG TTGACCCAGT CACAAGGATA CAAAACGCTG CGGAAGTGAG TTCCCTGCCT 
CTAACTAATG CACCATCAGA GTCCAGCTTC CCACCAGAAC CCCCACCTCA TTCACGGAGC 
AAAACGGAGC CGAGCCGTCG CACCCATGGC ACTGGCAAGC CCCATCGTTC CCATCATCGT 
CATCACTCCC CTAGCAAATC CAAAGGGAAG AAGTCTAAAG GAAGGCATGG ACACAAGCAG 
AATAAGAAAA AGGATGACAA GGAAAAAGAA GGCCATCGGC ATCGAAGCCG CCACCGCTCG 
CCTCATAGGG GGCAGGGCCG TTCACGTAGT GCAGACAACA TGCTGGATAG CCGTGATAGA 
GGGCACCGCC GAGGCCGTTC ACCAGAACGG CGCCACGGCC AACACTCCTC GAACCACCGC 
CACGGCTCTC CTCGACGCTC CCCTTATCGC CGCTCTCCAA CCCGCTCACC ATACCGACGC 
TCCCCATACC GCTCTCCTAG ACGCTCCCCT TACCGCTCTC ATCGCCACTC TCCTCGACGT 
TCTCCTCATC GCCACAGGTC CCCCAACCGC TACCACTCTC CAAACAGACA TCGCCACCGC 
TCTGCAGACC CCTACCGTTA TACCGCCACT GAGAGAAACA ATGAAAAGCC AACGGTAGAT 
GAGGCTGCAA TGACCGGGAC TCCAGAACCA AGCCTCCCTT TTGAAGAGAG TATCCTTCGG 
GAGTCGCCGG CCCTGGACCG CCTGAACAGG GACCCCACTC TTTCACTTCC ACCACTACAG 
AGAGAGGATC GTCTCTACGG GCAGGACAGT CTTCTAATGA GAACCTCCTC CATGGAAAGA 
GCCTACAGAG GAGACAGACT TGGCATGGCA TCCCGTGACA ACTTCAAAGA CGTCACCCTG 
CCCAGAGTCC GCACCCCTGA TTTTGATTCA TCCTACCAGA AGTACACCTC CCTGTTGAGA 
GGGCGTACTC CTGAGATGGT TGAGAGGGAT GGGCTCTTTG CCAGTCGAGG TAGCACCCCT 
GAACCCCGAT ACCGTGCTCG CAGCCTTGGA CGGGCCATCT CCCCAGCCAG GAGGGACGAC 
TCGGAGGATG AAGAAGATGA CGATCATTTT GTTGCGGCTA AGGTGAGAGA GTACTACAGC 
ACACTCAAGT CAGACACCAG CCGCCAAACC AAGCCTATGA TCCCTGAGCC TAAGAAGACC 
TACAAGGATA ATCCCAAAGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240754.1
CDS	388..5580
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385268.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385268.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GCTGGAAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGCC GCATCATCGA GGGGAGTCCA GCGGACCGCT GCGGGAAGCT GAAGGTGGGC 
GACCGCATCC TGGCTGTCAA CGGGAACTCC ATCACCAACA AGTCCCACTC TGACATCGTC 
AACCTGATCA AGGAGGCTGG GAACACCGTC ACGCTGCGGA TCATTCCTGG AGATGAGTCA 
TCCAATGCAT CTTTGTTGAC AAATGCAGAG AAGATTGCCA CCATAACCAC CACTCACACA 
GCTCAGCAAC AGGCAGCACC TGAGGCCAGG ACCAACACTA AACCAAAACA GGAATCTTAT 
GAGTTTAAAG CTCCTACGAA CACTGCTCCC CAGCCCCCCA TCCAGCAAGA CTCAGCTCAG 
GACTCCGAGT TCTACTCTGT GGACCTGGAG AGGGACAACA AAGGCTTTGG CTTCAGCCTG 
CGGGGAGGAA GAGAGTACAA CATGGACCTG TACGTGCTGA GGCTTGCTGA AGAAGGAGCT 
GCTGTTCGCA ATGGGAAAAT GAGGGTTGGA GATGAGATCT TGGAGATCAA CGGCGAAAGC 
ACCAAAGGCA TGAAGCACGC ACGGGCCATC GAGCTCATCA AGAACGGAGG CCGAAACGTC 
CACCTGGTGC TCAAGAGGGG TGACGGCTCC GTGCCTGAAT ATGACGGCAG CCCCAACGAC 
ATCAGCACAG TTGACCCAGT CACAAGGATA CAAAACGCTG CGGAAGTGAG TTCCCTGCCT 
CTAACTAATG CACCATCAGA GTCCAGCTTC CCACCAGAAC CCCCACCTCA TTCACGGAGC 
AAAACGGAGC CGAGCCGTCG CACCCATGGC ACTGGCAAGC CCCATCGTTC CCATCATCGT 
CATCACTCCC CTAGCAAATC CAAAGGGAAG AAGTCTAAAG GAAGGCATGG ACACAAGCAG 
AATAAGAAAA AGGATGACAA GGAAAAAGAA GGCCATCGGC ATCGAAGCCG CCACCGCTCG 
CCTCATAGGG GGCAGGGCCG TTCACGTAGT GCAGACAACA TGCTGGATAG CCGTGATAGA 
GGGCACCGCC GAGGCCGTTC ACCAGAACGG CGCCACGGCC AACACTCCTC GAACCACCGC 
CACGGCTCTC CTCGACGCTC CCCTTATCGC CGCTCTCCAA CCCGCTCACC ATACCGACGC 
TCCCCATACC GCTCTCCTAG ACGCTCCCCT TACCGCTCTC ATCGCCACTC TCCTCGACGT 
TCTCCTCATC GCCACAGGTC CCCCAACCGC TACCACTCTC CAAACAGACA TCGCCACCGC 
TCTGCAGACC CCTACCGTTA TACCGCCACT GAGAGAAACA ATGAAAAGCC AACGGTAGAT 
GAGGCTGCAA TGACCGGGAC TCCAGAACCA AGCCTCCCTT TTGAAGAGAG TATCCTTCGG 
GAGTCGCCGG CCCTGGACCG CCTGAACAGG GACCCCACTC TTTCACTTCC ACCACTACAG 
AGAGAGGATC GTCTCTACGG GCAGGACAGT CTTCTAATGA GAACCTCCTC CATGGAAAGA 
GCCTACAGAG GAGACAGACT TGGCATGGCA TCCCGTGACA ACTTCAAAGA CGTCACCCTG 
CCCAGAGTCC GCACCCCTGA TTTTGATTCA TCCTACCAGA AGTACACCTC CCTGTTGAGA 
GGGCGTACTC CTGAGATGGT TGAGAGGGAT GGGCTCTTTG CCAGTCGAGG TAGCACCCCT 
GAACCCCGAT ACCGTGCTCG CAGCCTTGGA CGGGCCATCT CCCCAGCCAG GAGGGACGAC 
TCGGAGGATG AAGAAGATGA CGATCATTTT GTTGCGGCTA AGGTGAGAGA GTACTACAGC 
ACACTCAAGT CAGACACCAG CCGCCAAACC AAGCCTATGA TCCCTGAGCC TAAGAAGACC 
TACAAGGATA ATCCCAAAGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385269.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240755.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X2, mRNA.

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CloneID	OCy15957
Clone ID Related Accession (Same CDS sequence)	XM_015385269.1
Accession Version	XM_015385269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAATTCCAGA CTTCCAGGAG CAGGACATTT TCCTGTGGAG GAAGGACACA 
GGCTTCGGCT TTAGGATCCT TGGTGGCAAC GAGCCAGGAG AACCTATCTA CATCGGCCAC 
ATAGTAAAGT ACGGCGCTGC AGATGAGGAC GGCCGCCTCC GATCCGGAGA TGAACTGATA 
TGCGTGGACG GCACGGCGGT GGTGGGCAAG TCGCATCAAT TGGTGGTGCA GTTGATGCAG 
CAGGCTGCCA AGCAGGGCCA TGTTAATCTT ACTGTGCGAC GCAAAACCAG CTACACAGTT 
AAATCAGAAG GAGACGTCCC TCCGTCACCA GCGTCCTCAC ACCACAGCAG CACCCAGGCG 
CCCAGCCTGA CGGAGGACAT GGGGAAGCGC ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC 
ACGGTCAGCT CGGGCAGCGG CTCCACCTCT GGCATCGGCA GCGGTGGAGG AGGGGGAGGC 
GGGGGGGCCG GCGTCACTGG GAATGCTGTG GTTTCCGCCT CGACGGTTGC GACCACATCC 
TCTCAGCCTC CCAACGGCAC CACAAACGCT GCCCCCGCCT CGGCCGTTCA GCCCTACGAC 
GTGGAGATCC GACGGGGCGA GAATGAAGGG TTCGGTTTCG TCATCGTGTC ATCAGTGTCG 
CGGCCTGAAG CTGGCACCAC CTTCGCTGGA AATGCATGTG TGGCCATGCC TCACAAAATA 
GGCCGCATCA TCGAGGGGAG TCCAGCGGAC CGCTGCGGGA AGCTGAAGGT GGGCGACCGC 
ATCCTGGCTG TCAACGGGAA CTCCATCACC AACAAGTCCC ACTCTGACAT CGTCAACCTG 
ATCAAGGAGG CTGGGAACAC CGTCACGCTG CGGATCATTC CTGGAGATGA GTCATCCAAT 
GCATCTTTGT TGACAAATGC AGAGAAGATT GCCACCATAA CCACCACTCA CACAGCTCAG 
CAACAGGCAG CACCTGAGGC CAGGACCAAC ACTAAACCAA AACAGGAATC TTATGAGTTT 
AAAGCTCCTA CGAACACTGC TCCCCAGCCC CCCATCCAGC AAGACTCAGC TCAGGACTCC 
GAGTTCTACT CTGTGGACCT GGAGAGGGAC AACAAAGGCT TTGGCTTCAG CCTGCGGGGA 
GGAAGAGAGT ACAACATGGA CCTGTACGTG CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT 
CGCAATGGGA AAATGAGGGT TGGAGATGAG ATCTTGGAGA TCAACGGCGA AAGCACCAAA 
GGCATGAAGC ACGCACGGGC CATCGAGCTC ATCAAGAACG GAGGCCGAAA CGTCCACCTG 
GTGCTCAAGA GGGGTGACGG CTCCGTGCCT GAATATGACG GCAGCCCCAA CGACATCAGC 
ACAGTTGACC CAGTCACAAG GATACAAAAC GCTGCGGAAG TGAGTTCCCT GCCTCTAACT 
AATGCACCAT CAGAGTCCAG CTTCCCACCA GAACCCCCAC CTCATTCACG GAGCAAAACG 
GAGCCGAGCC GTCGCACCCA TGGCACTGGC AAGCCCCATC GTTCCCATCA TCGTCATCAC 
TCCCCTAGCA AATCCAAAGG GAAGAAGTCT AAAGGAAGGC ATGGACACAA GCAGAATAAG 
AAAAAGGATG ACAAGGAAAA AGAAGGCCAT CGGCATCGAA GCCGCCACCG CTCGCCTCAT 
AGGGGGCAGG GCCGTTCACG TAGTGCAGAC AACATGCTGG ATAGCCGTGA TAGAGGGCAC 
CGCCGAGGCC GTTCACCAGA ACGGCGCCAC GGCCAACACT CCTCGAACCA CCGCCACGGC 
TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT CCAACCCGCT CACCATACCG ACGCTCCCCA 
TACCGCTCTC CTAGACGCTC CCCTTACCGC TCTCATCGCC ACTCTCCTCG ACGTTCTCCT 
CATCGCCACA GGTCCCCCAA CCGCTACCAC TCTCCAAACA GACATCGCCA CCGCTCTGCA 
GACCCCTACC GTTATACCGC CACTGAGAGA AACAATGAAA AGCCAACGGT AGATGAGGCT 
GCAATGACCG GGACTCCAGA ACCAAGCCTC CCTTTTGAAG AGAGTATCCT TCGGGAGTCG 
CCGGCCCTGG ACCGCCTGAA CAGGGACCCC ACTCTTTCAC TTCCACCACT ACAGAGAGAG 
GATCGTCTCT ACGGGCAGGA CAGTCTTCTA ATGAGAACCT CCTCCATGGA AAGAGCCTAC 
AGAGGAGACA GACTTGGCAT GGCATCCCGT GACAACTTCA AAGACGTCAC CCTGCCCAGA 
GTCCGCACCC CTGATTTTGA TTCATCCTAC CAGAAGTACA CCTCCCTGTT GAGAGGGCGT 
ACTCCTGAGA TGGTTGAGAG GGATGGGCTC TTTGCCAGTC GAGGTAGCAC CCCTGAACCC 
CGATACCGTG CTCGCAGCCT TGGACGGGCC ATCTCCCCAG CCAGGAGGGA CGACTCGGAG 
GATGAAGAAG ATGACGATCA TTTTGTTGCG GCTAAGGTGA GAGAGTACTA CAGCACACTC 
AAGTCAGACA CCAGCCGCCA AACCAAGCCT ATGATCCCTG AGCCTAAGAA GACCTACAAG 
GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240755.1
CDS	388..5574
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYEIPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385269.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385269.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAATTCCAGA CTTCCAGGAG CAGGACATTT TCCTGTGGAG GAAGGACACA 
GGCTTCGGCT TTAGGATCCT TGGTGGCAAC GAGCCAGGAG AACCTATCTA CATCGGCCAC 
ATAGTAAAGT ACGGCGCTGC AGATGAGGAC GGCCGCCTCC GATCCGGAGA TGAACTGATA 
TGCGTGGACG GCACGGCGGT GGTGGGCAAG TCGCATCAAT TGGTGGTGCA GTTGATGCAG 
CAGGCTGCCA AGCAGGGCCA TGTTAATCTT ACTGTGCGAC GCAAAACCAG CTACACAGTT 
AAATCAGAAG GAGACGTCCC TCCGTCACCA GCGTCCTCAC ACCACAGCAG CACCCAGGCG 
CCCAGCCTGA CGGAGGACAT GGGGAAGCGC ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC 
ACGGTCAGCT CGGGCAGCGG CTCCACCTCT GGCATCGGCA GCGGTGGAGG AGGGGGAGGC 
GGGGGGGCCG GCGTCACTGG GAATGCTGTG GTTTCCGCCT CGACGGTTGC GACCACATCC 
TCTCAGCCTC CCAACGGCAC CACAAACGCT GCCCCCGCCT CGGCCGTTCA GCCCTACGAC 
GTGGAGATCC GACGGGGCGA GAATGAAGGG TTCGGTTTCG TCATCGTGTC ATCAGTGTCG 
CGGCCTGAAG CTGGCACCAC CTTCGCTGGA AATGCATGTG TGGCCATGCC TCACAAAATA 
GGCCGCATCA TCGAGGGGAG TCCAGCGGAC CGCTGCGGGA AGCTGAAGGT GGGCGACCGC 
ATCCTGGCTG TCAACGGGAA CTCCATCACC AACAAGTCCC ACTCTGACAT CGTCAACCTG 
ATCAAGGAGG CTGGGAACAC CGTCACGCTG CGGATCATTC CTGGAGATGA GTCATCCAAT 
GCATCTTTGT TGACAAATGC AGAGAAGATT GCCACCATAA CCACCACTCA CACAGCTCAG 
CAACAGGCAG CACCTGAGGC CAGGACCAAC ACTAAACCAA AACAGGAATC TTATGAGTTT 
AAAGCTCCTA CGAACACTGC TCCCCAGCCC CCCATCCAGC AAGACTCAGC TCAGGACTCC 
GAGTTCTACT CTGTGGACCT GGAGAGGGAC AACAAAGGCT TTGGCTTCAG CCTGCGGGGA 
GGAAGAGAGT ACAACATGGA CCTGTACGTG CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT 
CGCAATGGGA AAATGAGGGT TGGAGATGAG ATCTTGGAGA TCAACGGCGA AAGCACCAAA 
GGCATGAAGC ACGCACGGGC CATCGAGCTC ATCAAGAACG GAGGCCGAAA CGTCCACCTG 
GTGCTCAAGA GGGGTGACGG CTCCGTGCCT GAATATGACG GCAGCCCCAA CGACATCAGC 
ACAGTTGACC CAGTCACAAG GATACAAAAC GCTGCGGAAG TGAGTTCCCT GCCTCTAACT 
AATGCACCAT CAGAGTCCAG CTTCCCACCA GAACCCCCAC CTCATTCACG GAGCAAAACG 
GAGCCGAGCC GTCGCACCCA TGGCACTGGC AAGCCCCATC GTTCCCATCA TCGTCATCAC 
TCCCCTAGCA AATCCAAAGG GAAGAAGTCT AAAGGAAGGC ATGGACACAA GCAGAATAAG 
AAAAAGGATG ACAAGGAAAA AGAAGGCCAT CGGCATCGAA GCCGCCACCG CTCGCCTCAT 
AGGGGGCAGG GCCGTTCACG TAGTGCAGAC AACATGCTGG ATAGCCGTGA TAGAGGGCAC 
CGCCGAGGCC GTTCACCAGA ACGGCGCCAC GGCCAACACT CCTCGAACCA CCGCCACGGC 
TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT CCAACCCGCT CACCATACCG ACGCTCCCCA 
TACCGCTCTC CTAGACGCTC CCCTTACCGC TCTCATCGCC ACTCTCCTCG ACGTTCTCCT 
CATCGCCACA GGTCCCCCAA CCGCTACCAC TCTCCAAACA GACATCGCCA CCGCTCTGCA 
GACCCCTACC GTTATACCGC CACTGAGAGA AACAATGAAA AGCCAACGGT AGATGAGGCT 
GCAATGACCG GGACTCCAGA ACCAAGCCTC CCTTTTGAAG AGAGTATCCT TCGGGAGTCG 
CCGGCCCTGG ACCGCCTGAA CAGGGACCCC ACTCTTTCAC TTCCACCACT ACAGAGAGAG 
GATCGTCTCT ACGGGCAGGA CAGTCTTCTA ATGAGAACCT CCTCCATGGA AAGAGCCTAC 
AGAGGAGACA GACTTGGCAT GGCATCCCGT GACAACTTCA AAGACGTCAC CCTGCCCAGA 
GTCCGCACCC CTGATTTTGA TTCATCCTAC CAGAAGTACA CCTCCCTGTT GAGAGGGCGT 
ACTCCTGAGA TGGTTGAGAG GGATGGGCTC TTTGCCAGTC GAGGTAGCAC CCCTGAACCC 
CGATACCGTG CTCGCAGCCT TGGACGGGCC ATCTCCCCAG CCAGGAGGGA CGACTCGGAG 
GATGAAGAAG ATGACGATCA TTTTGTTGCG GCTAAGGTGA GAGAGTACTA CAGCACACTC 
AAGTCAGACA CCAGCCGCCA AACCAAGCCT ATGATCCCTG AGCCTAAGAA GACCTACAAG 
GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385270.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240756.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X3, mRNA.

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CloneID	OCy15958
Clone ID Related Accession (Same CDS sequence)	XM_015385270.1
Accession Version	XM_015385270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGAATGGA TCGAGGACTC CGCATTGGCA 
GGCGCCCCAC TGGCTACCTA CACTGCCAAC CACGAGACCT ACAGGGACCC TCAAACAGGT 
CCGCCTGTTC CCAACGCCAT GGGACAAAAA CGTGGGAAGC CTTTCTTTAC CAGGAACCGA 
TCAGAACTGA ATGGAACATT CATCAATACC AAATTGAAGA AGAGCCGTCG GGGATTTGGG 
TTCACAGTTG TTGGAGGAGA CGAGCCAGAT GAGTTCTTGC AGATAAAGAG CTTGGTTCTG 
GACGGACCTG CAGCCCTCGA TGGCAAGATG GAGACAGGTG ATGTAATCGT GAGTGTAAAC 
GAAACCTGTG TCCTGGGCTA CACGCACGCC CAAGTTGTGA AGATCTTTCA GTCCATCCCA 
ATCGGCTCCA TGGTTAACTT GGAGCTGTGC CGTGGCTACC CACTGCCTTT CGACCCTGAC 
GACCCCAACA CCAGCTTGGT AACCTCAGTG GCCATCCTCG ACAACAAAGA TCCCATCATC 
GTCAACGGCC AGGAAGTCAC CAACAGCTAC GACTCCCCTT CCAGCCAGGG CAGTCAGAGC 
ACCAACAACG GCCCGACCAA CGGCGGGCCA CCTCTCAATG GCTTCACCCG ACCTCACAGC 
CCCTCCACTG AGGTGGCGTC TGATACCTCC TCGCAGCTCG GCTACTCTAG CGACGTGGTG 
AACCTGGCTT CGGCCATCGC CACGCAACCA GAACTTATCA CTGTACACAT GGAGAAAGGA 
GACAAGGGCT TTGGCTTCAC CATCGCTGAC AGCCTCGGGG GTGGAGGGCA GAGGGTGAAG 
CAGATTGTGG ACTACCCTCG CTGCCGCGGC CTCAGGGAGG GCGACATCAT CGTCGAGGTG 
AACAAGAGGA ATGTGCAGAG CATGTCTCAC AACCAGGTGG TGGACATGCT CAGCAAGTTA 
CCAAAAGGCA GCGAGGTCAC CATGCTGGTG CAGAGAGGAG TGGCACCTGC TAAGAAGAGC 
CCCAAACTGG ATTATTATGA CCGGGCTCCT CCATACAGAG ATTACACTAG GATGCAATTG 
TCCAGGAAGG ACAGTCAGAA CAGTTCCCAG CATAGCGTCT CCAGCCACCG GAGCGCCCAC 
ACCGACTCGC CTGTGCACTC GTCTCTGGCA CCCCCTCTCA GTGAGAGCAG CACGCCGCCG 
GCCCCGTCGC AGCCTTTGCC TGGCCTGCCT TCCCAGGACT CTCAAGGAGA CGGGACCACC 
CACCGAAAAC CAGACCCCTT TAAGATCTGG GCCCAGTCCA GGAGCATGTA TGAAAGTAGG 
CTTCCAGACT TCCAGGAGCA GGACATTTTC CTGTGGAGGA AGGACACAGG CTTCGGCTTT 
AGGATCCTTG GTGGCAACGA GCCAGGAGAA CCTATCTACA TCGGCCACAT AGTAAAGTAC 
GGCGCTGCAG ATGAGGACGG CCGCCTCCGA TCCGGAGATG AACTGATATG CGTGGACGGC 
ACGGCGGTGG TGGGCAAGTC GCATCAATTG GTGGTGCAGT TGATGCAGCA GGCTGCCAAG 
CAGGGCCATG TTAATCTTAC TGTGCGACGC AAAACCAGCT ACACAGTTAA ATCAGAAGGA 
GACGTCCCTC CGTCACCAGC GTCCTCACAC CACAGCAGCA CCCAGGCGCC CAGCCTGACG 
GAGGACATGG GGAAGCGCAC CCCGCAAGGC AGCCAGAATT CCCTCAACAC GGTCAGCTCG 
GGCAGCGGCT CCACCTCTGG CATCGGCAGC GGTGGAGGAG GGGGAGGCGG GGGGGCCGGC 
GTCACTGGGA ATGCTGTGGT TTCCGCCTCG ACGGTTGCGA CCACATCCTC TCAGCCTCCC 
AACGGCACCA CAAACGCTGC CCCCGCCTCG GCCGTTCAGC CCTACGACGT GGAGATCCGA 
CGGGGCGAGA ATGAAGGGTT CGGTTTCGTC ATCGTGTCAT CAGTGTCGCG GCCTGAAGCT 
GGCACCACCT TCGCTGGAAA TGCATGTGTG GCCATGCCTC ACAAAATAGG CCGCATCATC 
GAGGGGAGTC CAGCGGACCG CTGCGGGAAG CTGAAGGTGG GCGACCGCAT CCTGGCTGTC 
AACGGGAACT CCATCACCAA CAAGTCCCAC TCTGACATCG TCAACCTGAT CAAGGAGGCT 
GGGAACACCG TCACGCTGCG GATCATTCCT GGAGATGAGT CATCCAATGC ATCTTTGTTG 
ACAAATGCAG AGAAGATTGC CACCATAACC ACCACTCACA CAGCTCAGCA ACAGGCAGCA 
CCTGAGGCCA GGACCAACAC TAAACCAAAA CAGGAATCTT ATGAGTTTAA AGCTCCTACG 
AACACTGCTC CCCAGCCCCC CATCCAGCAA GACTCAGCTC AGGACTCCGA GTTCTACTCT 
GTGGACCTGG AGAGGGACAA CAAAGGCTTT GGCTTCAGCC TGCGGGGAGG AAGAGAGTAC 
AACATGGACC TGTACGTGCT GAGGCTTGCT GAAGAAGGAG CTGCTGTTCG CAATGGGAAA 
ATGAGGGTTG GAGATGAGAT CTTGGAGATC AACGGCGAAA GCACCAAAGG CATGAAGCAC 
GCACGGGCCA TCGAGCTCAT CAAGAACGGA GGCCGAAACG TCCACCTGGT GCTCAAGAGG 
GGTGACGGCT CCGTGCCTGA ATATGACGGC AGCCCCAACG ACATCAGCAC AGTTGACCCA 
GTCACAAGGA TACAAAACGC TGCGGAAGTG AGTTCCCTGC CTCTAACTAA TGCACCATCA 
GAGTCCAGCT TCCCACCAGA ACCCCCACCT CATTCACGGA GCAAAACGGA GCCGAGCCGT 
CGCACCCATG GCACTGGCAA GCCCCATCGT TCCCATCATC GTCATCACTC CCCTAGCAAA 
TCCAAAGGGA AGAAGTCTAA AGGAAGGCAT GGACACAAGC AGAATAAGAA AAAGGATGAC 
AAGGAAAAAG AAGGCCATCG GCATCGAAGC CGCCACCGCT CGCCTCATAG GGGGCAGGGC 
CGTTCACGTA GTGCAGACAA CATGCTGGAT AGCCGTGATA GAGGGCACCG CCGAGGCCGT 
TCACCAGAAC GGCGCCACGG CCAACACTCC TCGAACCACC GCCACGGCTC TCCTCGACGC 
TCCCCTTATC GCCGCTCTCC AACCCGCTCA CCATACCGAC GCTCCCCATA CCGCTCTCCT 
AGACGCTCCC CTTACCGCTC TCATCGCCAC TCTCCTCGAC GTTCTCCTCA TCGCCACAGG 
TCCCCCAACC GCTACCACTC TCCAAACAGA CATCGCCACC GCTCTGCAGA CCCCTACCGT 
TATACCGCCA CTGAGAGAAA CAATGAAAAG CCAACGGTAG ATGAGGCTGC AATGACCGGG 
ACTCCAGAAC CAAGCCTCCC TTTTGAAGAG AGTATCCTTC GGGAGTCGCC GGCCCTGGAC 
CGCCTGAACA GGGACCCCAC TCTTTCACTT CCACCACTAC AGAGAGAGGA TCGTCTCTAC 
GGGCAGGACA GTCTTCTAAT GAGAACCTCC TCCATGGAAA GAGCCTACAG AGGAGACAGA 
CTTGGCATGG CATCCCGTGA CAACTTCAAA GACGTCACCC TGCCCAGAGT CCGCACCCCT 
GATTTTGATT CATCCTACCA GAAGTACACC TCCCTGTTGA GAGGGCGTAC TCCTGAGATG 
GTTGAGAGGG ATGGGCTCTT TGCCAGTCGA GGTAGCACCC CTGAACCCCG ATACCGTGCT 
CGCAGCCTTG GACGGGCCAT CTCCCCAGCC AGGAGGGACG ACTCGGAGGA TGAAGAAGAT 
GACGATCATT TTGTTGCGGC TAAGGTGAGA GAGTACTACA GCACACTCAA GTCAGACACC 
AGCCGCCAAA CCAAGCCTAT GATCCCTGAG CCTAAGAAGA CCTACAAGGA TAATCCCAAA 
GACCTGAGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240756.1
CDS	388..5562
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEEWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385270.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385270.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGAATGGA TCGAGGACTC CGCATTGGCA 
GGCGCCCCAC TGGCTACCTA CACTGCCAAC CACGAGACCT ACAGGGACCC TCAAACAGGT 
CCGCCTGTTC CCAACGCCAT GGGACAAAAA CGTGGGAAGC CTTTCTTTAC CAGGAACCGA 
TCAGAACTGA ATGGAACATT CATCAATACC AAATTGAAGA AGAGCCGTCG GGGATTTGGG 
TTCACAGTTG TTGGAGGAGA CGAGCCAGAT GAGTTCTTGC AGATAAAGAG CTTGGTTCTG 
GACGGACCTG CAGCCCTCGA TGGCAAGATG GAGACAGGTG ATGTAATCGT GAGTGTAAAC 
GAAACCTGTG TCCTGGGCTA CACGCACGCC CAAGTTGTGA AGATCTTTCA GTCCATCCCA 
ATCGGCTCCA TGGTTAACTT GGAGCTGTGC CGTGGCTACC CACTGCCTTT CGACCCTGAC 
GACCCCAACA CCAGCTTGGT AACCTCAGTG GCCATCCTCG ACAACAAAGA TCCCATCATC 
GTCAACGGCC AGGAAGTCAC CAACAGCTAC GACTCCCCTT CCAGCCAGGG CAGTCAGAGC 
ACCAACAACG GCCCGACCAA CGGCGGGCCA CCTCTCAATG GCTTCACCCG ACCTCACAGC 
CCCTCCACTG AGGTGGCGTC TGATACCTCC TCGCAGCTCG GCTACTCTAG CGACGTGGTG 
AACCTGGCTT CGGCCATCGC CACGCAACCA GAACTTATCA CTGTACACAT GGAGAAAGGA 
GACAAGGGCT TTGGCTTCAC CATCGCTGAC AGCCTCGGGG GTGGAGGGCA GAGGGTGAAG 
CAGATTGTGG ACTACCCTCG CTGCCGCGGC CTCAGGGAGG GCGACATCAT CGTCGAGGTG 
AACAAGAGGA ATGTGCAGAG CATGTCTCAC AACCAGGTGG TGGACATGCT CAGCAAGTTA 
CCAAAAGGCA GCGAGGTCAC CATGCTGGTG CAGAGAGGAG TGGCACCTGC TAAGAAGAGC 
CCCAAACTGG ATTATTATGA CCGGGCTCCT CCATACAGAG ATTACACTAG GATGCAATTG 
TCCAGGAAGG ACAGTCAGAA CAGTTCCCAG CATAGCGTCT CCAGCCACCG GAGCGCCCAC 
ACCGACTCGC CTGTGCACTC GTCTCTGGCA CCCCCTCTCA GTGAGAGCAG CACGCCGCCG 
GCCCCGTCGC AGCCTTTGCC TGGCCTGCCT TCCCAGGACT CTCAAGGAGA CGGGACCACC 
CACCGAAAAC CAGACCCCTT TAAGATCTGG GCCCAGTCCA GGAGCATGTA TGAAAGTAGG 
CTTCCAGACT TCCAGGAGCA GGACATTTTC CTGTGGAGGA AGGACACAGG CTTCGGCTTT 
AGGATCCTTG GTGGCAACGA GCCAGGAGAA CCTATCTACA TCGGCCACAT AGTAAAGTAC 
GGCGCTGCAG ATGAGGACGG CCGCCTCCGA TCCGGAGATG AACTGATATG CGTGGACGGC 
ACGGCGGTGG TGGGCAAGTC GCATCAATTG GTGGTGCAGT TGATGCAGCA GGCTGCCAAG 
CAGGGCCATG TTAATCTTAC TGTGCGACGC AAAACCAGCT ACACAGTTAA ATCAGAAGGA 
GACGTCCCTC CGTCACCAGC GTCCTCACAC CACAGCAGCA CCCAGGCGCC CAGCCTGACG 
GAGGACATGG GGAAGCGCAC CCCGCAAGGC AGCCAGAATT CCCTCAACAC GGTCAGCTCG 
GGCAGCGGCT CCACCTCTGG CATCGGCAGC GGTGGAGGAG GGGGAGGCGG GGGGGCCGGC 
GTCACTGGGA ATGCTGTGGT TTCCGCCTCG ACGGTTGCGA CCACATCCTC TCAGCCTCCC 
AACGGCACCA CAAACGCTGC CCCCGCCTCG GCCGTTCAGC CCTACGACGT GGAGATCCGA 
CGGGGCGAGA ATGAAGGGTT CGGTTTCGTC ATCGTGTCAT CAGTGTCGCG GCCTGAAGCT 
GGCACCACCT TCGCTGGAAA TGCATGTGTG GCCATGCCTC ACAAAATAGG CCGCATCATC 
GAGGGGAGTC CAGCGGACCG CTGCGGGAAG CTGAAGGTGG GCGACCGCAT CCTGGCTGTC 
AACGGGAACT CCATCACCAA CAAGTCCCAC TCTGACATCG TCAACCTGAT CAAGGAGGCT 
GGGAACACCG TCACGCTGCG GATCATTCCT GGAGATGAGT CATCCAATGC ATCTTTGTTG 
ACAAATGCAG AGAAGATTGC CACCATAACC ACCACTCACA CAGCTCAGCA ACAGGCAGCA 
CCTGAGGCCA GGACCAACAC TAAACCAAAA CAGGAATCTT ATGAGTTTAA AGCTCCTACG 
AACACTGCTC CCCAGCCCCC CATCCAGCAA GACTCAGCTC AGGACTCCGA GTTCTACTCT 
GTGGACCTGG AGAGGGACAA CAAAGGCTTT GGCTTCAGCC TGCGGGGAGG AAGAGAGTAC 
AACATGGACC TGTACGTGCT GAGGCTTGCT GAAGAAGGAG CTGCTGTTCG CAATGGGAAA 
ATGAGGGTTG GAGATGAGAT CTTGGAGATC AACGGCGAAA GCACCAAAGG CATGAAGCAC 
GCACGGGCCA TCGAGCTCAT CAAGAACGGA GGCCGAAACG TCCACCTGGT GCTCAAGAGG 
GGTGACGGCT CCGTGCCTGA ATATGACGGC AGCCCCAACG ACATCAGCAC AGTTGACCCA 
GTCACAAGGA TACAAAACGC TGCGGAAGTG AGTTCCCTGC CTCTAACTAA TGCACCATCA 
GAGTCCAGCT TCCCACCAGA ACCCCCACCT CATTCACGGA GCAAAACGGA GCCGAGCCGT 
CGCACCCATG GCACTGGCAA GCCCCATCGT TCCCATCATC GTCATCACTC CCCTAGCAAA 
TCCAAAGGGA AGAAGTCTAA AGGAAGGCAT GGACACAAGC AGAATAAGAA AAAGGATGAC 
AAGGAAAAAG AAGGCCATCG GCATCGAAGC CGCCACCGCT CGCCTCATAG GGGGCAGGGC 
CGTTCACGTA GTGCAGACAA CATGCTGGAT AGCCGTGATA GAGGGCACCG CCGAGGCCGT 
TCACCAGAAC GGCGCCACGG CCAACACTCC TCGAACCACC GCCACGGCTC TCCTCGACGC 
TCCCCTTATC GCCGCTCTCC AACCCGCTCA CCATACCGAC GCTCCCCATA CCGCTCTCCT 
AGACGCTCCC CTTACCGCTC TCATCGCCAC TCTCCTCGAC GTTCTCCTCA TCGCCACAGG 
TCCCCCAACC GCTACCACTC TCCAAACAGA CATCGCCACC GCTCTGCAGA CCCCTACCGT 
TATACCGCCA CTGAGAGAAA CAATGAAAAG CCAACGGTAG ATGAGGCTGC AATGACCGGG 
ACTCCAGAAC CAAGCCTCCC TTTTGAAGAG AGTATCCTTC GGGAGTCGCC GGCCCTGGAC 
CGCCTGAACA GGGACCCCAC TCTTTCACTT CCACCACTAC AGAGAGAGGA TCGTCTCTAC 
GGGCAGGACA GTCTTCTAAT GAGAACCTCC TCCATGGAAA GAGCCTACAG AGGAGACAGA 
CTTGGCATGG CATCCCGTGA CAACTTCAAA GACGTCACCC TGCCCAGAGT CCGCACCCCT 
GATTTTGATT CATCCTACCA GAAGTACACC TCCCTGTTGA GAGGGCGTAC TCCTGAGATG 
GTTGAGAGGG ATGGGCTCTT TGCCAGTCGA GGTAGCACCC CTGAACCCCG ATACCGTGCT 
CGCAGCCTTG GACGGGCCAT CTCCCCAGCC AGGAGGGACG ACTCGGAGGA TGAAGAAGAT 
GACGATCATT TTGTTGCGGC TAAGGTGAGA GAGTACTACA GCACACTCAA GTCAGACACC 
AGCCGCCAAA CCAAGCCTAT GATCCCTGAG CCTAAGAAGA CCTACAAGGA TAATCCCAAA 
GACCTGAGCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385271.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240757.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCy15959
Clone ID Related Accession (Same CDS sequence)	XM_015385271.1
Accession Version	XM_015385271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240757.1
CDS	388..5544
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385271.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385271.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGGAT TATTATGACC GGGCTCCTCC ATACAGAGAT 
TACACTAGGA TGCAATTGTC CAGGAAGGAC AGTCAGAACA GTTCCCAGCA TAGCGTCTCC 
AGCCACCGGA GCGCCCACAC CGACTCGCCT GTGCACTCGT CTCTGGCACC CCCTCTCAGT 
GAGAGCAGCA CGCCGCCGGC CCCGTCGCAG CCTTTGCCTG GCCTGCCTTC CCAGGACTCT 
CAAGGAGACG GGACCACCCA CCGAAAACCA GACCCCTTTA AGATCTGGGC CCAGTCCAGG 
AGCATGTATG AAAGTAGGCT TCCAGACTTC CAGGAGCAGG ACATTTTCCT GTGGAGGAAG 
GACACAGGCT TCGGCTTTAG GATCCTTGGT GGCAACGAGC CAGGAGAACC TATCTACATC 
GGCCACATAG TAAAGTACGG CGCTGCAGAT GAGGACGGCC GCCTCCGATC CGGAGATGAA 
CTGATATGCG TGGACGGCAC GGCGGTGGTG GGCAAGTCGC ATCAATTGGT GGTGCAGTTG 
ATGCAGCAGG CTGCCAAGCA GGGCCATGTT AATCTTACTG TGCGACGCAA AACCAGCTAC 
ACAGTTAAAT CAGAAGGAGA CGTCCCTCCG TCACCAGCGT CCTCACACCA CAGCAGCACC 
CAGGCGCCCA GCCTGACGGA GGACATGGGG AAGCGCACCC CGCAAGGCAG CCAGAATTCC 
CTCAACACGG TCAGCTCGGG CAGCGGCTCC ACCTCTGGCA TCGGCAGCGG TGGAGGAGGG 
GGAGGCGGGG GGGCCGGCGT CACTGGGAAT GCTGTGGTTT CCGCCTCGAC GGTTGCGACC 
ACATCCTCTC AGCCTCCCAA CGGCACCACA AACGCTGCCC CCGCCTCGGC CGTTCAGCCC 
TACGACGTGG AGATCCGACG GGGCGAGAAT GAAGGGTTCG GTTTCGTCAT CGTGTCATCA 
GTGTCGCGGC CTGAAGCTGG CACCACCTTC GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385272.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240758.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X5, mRNA.

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CloneID	OCy15960
Clone ID Related Accession (Same CDS sequence)	XM_015385272.1
Accession Version	XM_015385272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGAC 
GGCAGCCCCA ACGACATCAG CACAGTTGAC CCAGTCACAA GGATACAAAA CGCTGCGGAA 
GTGAGTTCCC TGCCTCTAAC TAATGCACCA TCAGAGTCCA GCTTCCCACC AGAACCCCCA 
CCTCATTCAC GGAGCAAAAC GGAGCCGAGC CGTCGCACCC ATGGCACTGG CAAGCCCCAT 
CGTTCCCATC ATCGTCATCA CTCCCCTAGC AAATCCAAAG GGAAGAAGTC TAAAGGAAGG 
CATGGACACA AGCAGAATAA GAAAAAGGAT GACAAGGAAA AAGAAGGCCA TCGGCATCGA 
AGCCGCCACC GCTCGCCTCA TAGGGGGCAG GGCCGTTCAC GTAGTGCAGA CAACATGCTG 
GATAGCCGTG ATAGAGGGCA CCGCCGAGGC CGTTCACCAG AACGGCGCCA CGGCCAACAC 
TCCTCGAACC ACCGCCACGG CTCTCCTCGA CGCTCCCCTT ATCGCCGCTC TCCAACCCGC 
TCACCATACC GACGCTCCCC ATACCGCTCT CCTAGACGCT CCCCTTACCG CTCTCATCGC 
CACTCTCCTC GACGTTCTCC TCATCGCCAC AGGTCCCCCA ACCGCTACCA CTCTCCAAAC 
AGACATCGCC ACCGCTCTGC AGACCCCTAC CGTTATACCG CCACTGAGAG AAACAATGAA 
AAGCCAACGG TAGATGAGGC TGCAATGACC GGGACTCCAG AACCAAGCCT CCCTTTTGAA 
GAGAGTATCC TTCGGGAGTC GCCGGCCCTG GACCGCCTGA ACAGGGACCC CACTCTTTCA 
CTTCCACCAC TACAGAGAGA GGATCGTCTC TACGGGCAGG ACAGTCTTCT AATGAGAACC 
TCCTCCATGG AAAGAGCCTA CAGAGGAGAC AGACTTGGCA TGGCATCCCG TGACAACTTC 
AAAGACGTCA CCCTGCCCAG AGTCCGCACC CCTGATTTTG ATTCATCCTA CCAGAAGTAC 
ACCTCCCTGT TGAGAGGGCG TACTCCTGAG ATGGTTGAGA GGGATGGGCT CTTTGCCAGT 
CGAGGTAGCA CCCCTGAACC CCGATACCGT GCTCGCAGCC TTGGACGGGC CATCTCCCCA 
GCCAGGAGGG ACGACTCGGA GGATGAAGAA GATGACGATC ATTTTGTTGC GGCTAAGGTG 
AGAGAGTACT ACAGCACACT CAAGTCAGAC ACCAGCCGCC AAACCAAGCC TATGATCCCT 
GAGCCTAAGA AGACCTACAA GGATAATCCC AAAGACCTGA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240758.1
CDS	388..5535
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385272.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385272.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG TGGGAAGCCT 
TTCTTTACCA GGAACCGATC AGAACTGAAT GGAACATTCA TCAATACCAA ATTGAAGAAG 
AGCCGTCGGG GATTTGGGTT CACAGTTGTT GGAGGAGACG AGCCAGATGA GTTCTTGCAG 
ATAAAGAGCT TGGTTCTGGA CGGACCTGCA GCCCTCGATG GCAAGATGGA GACAGGTGAT 
GTAATCGTGA GTGTAAACGA AACCTGTGTC CTGGGCTACA CGCACGCCCA AGTTGTGAAG 
ATCTTTCAGT CCATCCCAAT CGGCTCCATG GTTAACTTGG AGCTGTGCCG TGGCTACCCA 
CTGCCTTTCG ACCCTGACGA CCCCAACACC AGCTTGGTAA CCTCAGTGGC CATCCTCGAC 
AACAAAGATC CCATCATCGT CAACGGCCAG GAAGTCACCA ACAGCTACGA CTCCCCTTCC 
AGCCAGGGCA GTCAGAGCAC CAACAACGGC CCGACCAACG GCGGGCCACC TCTCAATGGC 
TTCACCCGAC CTCACAGCCC CTCCACTGAG GTGGCGTCTG ATACCTCCTC GCAGCTCGGC 
TACTCTAGCG ACGTGGTGAA CCTGGCTTCG GCCATCGCCA CGCAACCAGA ACTTATCACT 
GTACACATGG AGAAAGGAGA CAAGGGCTTT GGCTTCACCA TCGCTGACAG CCTCGGGGGT 
GGAGGGCAGA GGGTGAAGCA GATTGTGGAC TACCCTCGCT GCCGCGGCCT CAGGGAGGGC 
GACATCATCG TCGAGGTGAA CAAGAGGAAT GTGCAGAGCA TGTCTCACAA CCAGGTGGTG 
GACATGCTCA GCAAGTTACC AAAAGGCAGC GAGGTCACCA TGCTGGTGCA GAGAGGAGTG 
GCACCTGCTA AGAAGAGCCC CAAACTGCAA TTGTCCAGGA AGGACAGTCA GAACAGTTCC 
CAGCATAGCG TCTCCAGCCA CCGGAGCGCC CACACCGACT CGCCTGTGCA CTCGTCTCTG 
GCACCCCCTC TCAGTGAGAG CAGCACGCCG CCGGCCCCGT CGCAGCCTTT GCCTGGCCTG 
CCTTCCCAGG ACTCTCAAGG AGACGGGACC ACCCACCGAA AACCAGACCC CTTTAAGATC 
TGGGCCCAGT CCAGGAGCAT GTATGAAAGT AGGCTTCCAG ACTTCCAGGA GCAGGACATT 
TTCCTGTGGA GGAAGGACAC AGGCTTCGGC TTTAGGATCC TTGGTGGCAA CGAGCCAGGA 
GAACCTATCT ACATCGGCCA CATAGTAAAG TACGGCGCTG CAGATGAGGA CGGCCGCCTC 
CGATCCGGAG ATGAACTGAT ATGCGTGGAC GGCACGGCGG TGGTGGGCAA GTCGCATCAA 
TTGGTGGTGC AGTTGATGCA GCAGGCTGCC AAGCAGGGCC ATGTTAATCT TACTGTGCGA 
CGCAAAACCA GCTACACAGT TAAATCAGAA GGAGACGTCC CTCCGTCACC AGCGTCCTCA 
CACCACAGCA GCACCCAGGC GCCCAGCCTG ACGGAGGACA TGGGGAAGCG CACCCCGCAA 
GGCAGCCAGA ATTCCCTCAA CACGGTCAGC TCGGGCAGCG GCTCCACCTC TGGCATCGGC 
AGCGGTGGAG GAGGGGGAGG CGGGGGGGCC GGCGTCACTG GGAATGCTGT GGTTTCCGCC 
TCGACGGTTG CGACCACATC CTCTCAGCCT CCCAACGGCA CCACAAACGC TGCCCCCGCC 
TCGGCCGTTC AGCCCTACGA CGTGGAGATC CGACGGGGCG AGAATGAAGG GTTCGGTTTC 
GTCATCGTGT CATCAGTGTC GCGGCCTGAA GCTGGCACCA CCTTCGCTGG AAATGCATGT 
GTGGCCATGC CTCACAAAAT AGGCCGCATC ATCGAGGGGA GTCCAGCGGA CCGCTGCGGG 
AAGCTGAAGG TGGGCGACCG CATCCTGGCT GTCAACGGGA ACTCCATCAC CAACAAGTCC 
CACTCTGACA TCGTCAACCT GATCAAGGAG GCTGGGAACA CCGTCACGCT GCGGATCATT 
CCTGGAGATG AGTCATCCAA TGCATCTTTG TTGACAAATG CAGAGAAGAT TGCCACCATA 
ACCACCACTC ACACAGCTCA GCAACAGGCA GCACCTGAGG CCAGGACCAA CACTAAACCA 
AAACAGGAAT CTTATGAGTT TAAAGCTCCT ACGAACACTG CTCCCCAGCC CCCCATCCAG 
CAAGACTCAG CTCAGGACTC CGAGTTCTAC TCTGTGGACC TGGAGAGGGA CAACAAAGGC 
TTTGGCTTCA GCCTGCGGGG AGGAAGAGAG TACAACATGG ACCTGTACGT GCTGAGGCTT 
GCTGAAGAAG GAGCTGCTGT TCGCAATGGG AAAATGAGGG TTGGAGATGA GATCTTGGAG 
ATCAACGGCG AAAGCACCAA AGGCATGAAG CACGCACGGG CCATCGAGCT CATCAAGAAC 
GGAGGCCGAA ACGTCCACCT GGTGCTCAAG AGGGGTGACG GCTCCGTGCC TGAATATGAC 
GGCAGCCCCA ACGACATCAG CACAGTTGAC CCAGTCACAA GGATACAAAA CGCTGCGGAA 
GTGAGTTCCC TGCCTCTAAC TAATGCACCA TCAGAGTCCA GCTTCCCACC AGAACCCCCA 
CCTCATTCAC GGAGCAAAAC GGAGCCGAGC CGTCGCACCC ATGGCACTGG CAAGCCCCAT 
CGTTCCCATC ATCGTCATCA CTCCCCTAGC AAATCCAAAG GGAAGAAGTC TAAAGGAAGG 
CATGGACACA AGCAGAATAA GAAAAAGGAT GACAAGGAAA AAGAAGGCCA TCGGCATCGA 
AGCCGCCACC GCTCGCCTCA TAGGGGGCAG GGCCGTTCAC GTAGTGCAGA CAACATGCTG 
GATAGCCGTG ATAGAGGGCA CCGCCGAGGC CGTTCACCAG AACGGCGCCA CGGCCAACAC 
TCCTCGAACC ACCGCCACGG CTCTCCTCGA CGCTCCCCTT ATCGCCGCTC TCCAACCCGC 
TCACCATACC GACGCTCCCC ATACCGCTCT CCTAGACGCT CCCCTTACCG CTCTCATCGC 
CACTCTCCTC GACGTTCTCC TCATCGCCAC AGGTCCCCCA ACCGCTACCA CTCTCCAAAC 
AGACATCGCC ACCGCTCTGC AGACCCCTAC CGTTATACCG CCACTGAGAG AAACAATGAA 
AAGCCAACGG TAGATGAGGC TGCAATGACC GGGACTCCAG AACCAAGCCT CCCTTTTGAA 
GAGAGTATCC TTCGGGAGTC GCCGGCCCTG GACCGCCTGA ACAGGGACCC CACTCTTTCA 
CTTCCACCAC TACAGAGAGA GGATCGTCTC TACGGGCAGG ACAGTCTTCT AATGAGAACC 
TCCTCCATGG AAAGAGCCTA CAGAGGAGAC AGACTTGGCA TGGCATCCCG TGACAACTTC 
AAAGACGTCA CCCTGCCCAG AGTCCGCACC CCTGATTTTG ATTCATCCTA CCAGAAGTAC 
ACCTCCCTGT TGAGAGGGCG TACTCCTGAG ATGGTTGAGA GGGATGGGCT CTTTGCCAGT 
CGAGGTAGCA CCCCTGAACC CCGATACCGT GCTCGCAGCC TTGGACGGGC CATCTCCCCA 
GCCAGGAGGG ACGACTCGGA GGATGAAGAA GATGACGATC ATTTTGTTGC GGCTAAGGTG 
AGAGAGTACT ACAGCACACT CAAGTCAGAC ACCAGCCGCC AAACCAAGCC TATGATCCCT 
GAGCCTAAGA AGACCTACAA GGATAATCCC AAAGACCTGA GCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385273.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240759.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X6, mRNA.

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CloneID	OCy15961
Clone ID Related Accession (Same CDS sequence)	XM_015385273.1 , XM_015385274.1
Accession Version	XM_015385273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CTGGAATAGG GCTTACAGAT AACCAACTGG AGGACGACGA GGACCTCCCT 
GACATGAACA GCAGCTTTAC AGGAGATTCT AGCGAAATAG ACGAGCTTCA TCACTCAGCG 
CGCCCCCACG TCCCTCGCTA TAGTGACCCG TCCTACGCTG GGACAACCCC CTCACCATCG 
GCCACCACGG AGAGCACACA GCAGACAAAC CTTCACCTGA GCCATCCTCC CCCGGAGGAC 
CCGCTGGGAC CTCTGCCTGA CAACTGGGAG ATGGCCTACA CCGAGAATGG AGAGGTCTAC 
TTTATAGACC ACAATACAAA GACCACCTCT TGGTTAGACC CTCGGTGCCT GGACAAACCT 
CAGAAACCCC TGGAAGAATG TGAAGATGAT GAAGGGGTCC ATACAGAAGA GCTGGATGAT 
CTCGAACTTC CTCCAGGATG GGAGAAAATT GATGATCCAG TCTATGGGGT CTACTATGTT 
GATCATATCA ACAGGAAGAC CCAGTATGAA AACCCAGTGC TGGAGGCTAA GAGGCGCAGG 
CAGCTGGAAC AGCAGCCCGC CCAGCAGCCT CAGCCCCAAA GCCAGCAGCC ACCTGAAGGT 
GAGCGCTACA TCCGAGAATG GATCGAGGAC TCCGCATTGG CAGGCGCCCC ACTGGCTACC 
TACACTGCCA ACCACGAGAC CTACAGGGAC CCTCAAACAG GTCCGCCTGT TCCCAACGCC 
ATGGGACAAA AACGTGGGAA GCCTTTCTTT ACCAGGAACC GATCAGAACT GAATGGAACA 
TTCATCAATA CCAAATTGAA GAAGAGCCGT CGGGGATTTG GGTTCACAGT TGTTGGAGGA 
GACGAGCCAG ATGAGTTCTT GCAGATAAAG AGCTTGGTTC TGGACGGACC TGCAGCCCTC 
GATGGCAAGA TGGAGACAGG TGATGTAATC GTGAGTGTAA ACGAAACCTG TGTCCTGGGC 
TACACGCACG CCCAAGTTGT GAAGATCTTT CAGTCCATCC CAATCGGCTC CATGGTTAAC 
TTGGAGCTGT GCCGTGGCTA CCCACTGCCT TTCGACCCTG ACGACCCCAA CACCAGCTTG 
GTAACCTCAG TGGCCATCCT CGACAACAAA GATCCCATCA TCGTCAACGG CCAGGAAGTC 
ACCAACAGCT ACGACTCCCC TTCCAGCCAG GGCAGTCAGA GCACCAACAA CGGCCCGACC 
AACGGCGGGC CACCTCTCAA TGGCTTCACC CGACCTCACA GCCCCTCCAC TGAGGTGGCG 
TCTGATACCT CCTCGCAGCT CGGCTACTCT AGCGACGTGG TGAACCTGGC TTCGGCCATC 
GCCACGCAAC CAGAACTTAT CACTGTACAC ATGGAGAAAG GAGACAAGGG CTTTGGCTTC 
ACCATCGCTG ACAGCCTCGG GGGTGGAGGG CAGAGGGTGA AGCAGATTGT GGACTACCCT 
CGCTGCCGCG GCCTCAGGGA GGGCGACATC ATCGTCGAGG TGAACAAGAG GAATGTGCAG 
AGCATGTCTC ACAACCAGGT GGTGGACATG CTCAGCAAGT TACCAAAAGG CAGCGAGGTC 
ACCATGCTGG TGCAGAGAGG AGTGGCACCT GCTAAGAAGA GCCCCAAACT GGATTATTAT 
GACCGGGCTC CTCCATACAG AGATTACACT AGGATGCAAT TGTCCAGGAA GGACAGTCAG 
AACAGTTCCC AGCATAGCGT CTCCAGCCAC CGGAGCGCCC ACACCGACTC GCCTGTGCAC 
TCGTCTCTGG CACCCCCTCT CAGTGAGAGC AGCACGCCGC CGGCCCCGTC GCAGCCTTTG 
CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA GACGGGACCA CCCACCGAAA ACCAGACCCC 
TTTAAGATCT GGGCCCAGTC CAGGAGCATG TATGAAAGTA GGCTTCCAGA CTTCCAGGAG 
CAGGACATTT TCCTGTGGAG GAAGGACACA GGCTTCGGCT TTAGGATCCT TGGTGGCAAC 
GAGCCAGGAG AACCTATCTA CATCGGCCAC ATAGTAAAGT ACGGCGCTGC AGATGAGGAC 
GGCCGCCTCC GATCCGGAGA TGAACTGATA TGCGTGGACG GCACGGCGGT GGTGGGCAAG 
TCGCATCAAT TGGTGGTGCA GTTGATGCAG CAGGCTGCCA AGCAGGGCCA TGTTAATCTT 
ACTGTGCGAC GCAAAACCAG CTACACAGTT AAATCAGAAG GAGACGTCCC TCCGTCACCA 
GCGTCCTCAC ACCACAGCAG CACCCAGGCG CCCAGCCTGA CGGAGGACAT GGGGAAGCGC 
ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC ACGGTCAGCT CGGGCAGCGG CTCCACCTCT 
GGCATCGGCA GCGGTGGAGG AGGGGGAGGC GGGGGGGCCG GCGTCACTGG GAATGCTGTG 
GTTTCCGCCT CGACGGTTGC GACCACATCC TCTCAGCCTC CCAACGGCAC CACAAACGCT 
GCCCCCGCCT CGGCCGTTCA GCCCTACGAC GTGGAGATCC GACGGGGCGA GAATGAAGGG 
TTCGGTTTCG TCATCGTGTC ATCAGTGTCG CGGCCTGAAG CTGGCACCAC CTTCGCTGGA 
AATGCATGTG TGGCCATGCC TCACAAAATA GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240759.1
CDS	575..5071
Translation	MQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385273.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385273.1

ORF Insert Sequence:

ATGCAAAATG CTGGAATAGG GCTTACAGAT AACCAACTGG AGGACGACGA GGACCTCCCT 
GACATGAACA GCAGCTTTAC AGGAGATTCT AGCGAAATAG ACGAGCTTCA TCACTCAGCG 
CGCCCCCACG TCCCTCGCTA TAGTGACCCG TCCTACGCTG GGACAACCCC CTCACCATCG 
GCCACCACGG AGAGCACACA GCAGACAAAC CTTCACCTGA GCCATCCTCC CCCGGAGGAC 
CCGCTGGGAC CTCTGCCTGA CAACTGGGAG ATGGCCTACA CCGAGAATGG AGAGGTCTAC 
TTTATAGACC ACAATACAAA GACCACCTCT TGGTTAGACC CTCGGTGCCT GGACAAACCT 
CAGAAACCCC TGGAAGAATG TGAAGATGAT GAAGGGGTCC ATACAGAAGA GCTGGATGAT 
CTCGAACTTC CTCCAGGATG GGAGAAAATT GATGATCCAG TCTATGGGGT CTACTATGTT 
GATCATATCA ACAGGAAGAC CCAGTATGAA AACCCAGTGC TGGAGGCTAA GAGGCGCAGG 
CAGCTGGAAC AGCAGCCCGC CCAGCAGCCT CAGCCCCAAA GCCAGCAGCC ACCTGAAGGT 
GAGCGCTACA TCCGAGAATG GATCGAGGAC TCCGCATTGG CAGGCGCCCC ACTGGCTACC 
TACACTGCCA ACCACGAGAC CTACAGGGAC CCTCAAACAG GTCCGCCTGT TCCCAACGCC 
ATGGGACAAA AACGTGGGAA GCCTTTCTTT ACCAGGAACC GATCAGAACT GAATGGAACA 
TTCATCAATA CCAAATTGAA GAAGAGCCGT CGGGGATTTG GGTTCACAGT TGTTGGAGGA 
GACGAGCCAG ATGAGTTCTT GCAGATAAAG AGCTTGGTTC TGGACGGACC TGCAGCCCTC 
GATGGCAAGA TGGAGACAGG TGATGTAATC GTGAGTGTAA ACGAAACCTG TGTCCTGGGC 
TACACGCACG CCCAAGTTGT GAAGATCTTT CAGTCCATCC CAATCGGCTC CATGGTTAAC 
TTGGAGCTGT GCCGTGGCTA CCCACTGCCT TTCGACCCTG ACGACCCCAA CACCAGCTTG 
GTAACCTCAG TGGCCATCCT CGACAACAAA GATCCCATCA TCGTCAACGG CCAGGAAGTC 
ACCAACAGCT ACGACTCCCC TTCCAGCCAG GGCAGTCAGA GCACCAACAA CGGCCCGACC 
AACGGCGGGC CACCTCTCAA TGGCTTCACC CGACCTCACA GCCCCTCCAC TGAGGTGGCG 
TCTGATACCT CCTCGCAGCT CGGCTACTCT AGCGACGTGG TGAACCTGGC TTCGGCCATC 
GCCACGCAAC CAGAACTTAT CACTGTACAC ATGGAGAAAG GAGACAAGGG CTTTGGCTTC 
ACCATCGCTG ACAGCCTCGG GGGTGGAGGG CAGAGGGTGA AGCAGATTGT GGACTACCCT 
CGCTGCCGCG GCCTCAGGGA GGGCGACATC ATCGTCGAGG TGAACAAGAG GAATGTGCAG 
AGCATGTCTC ACAACCAGGT GGTGGACATG CTCAGCAAGT TACCAAAAGG CAGCGAGGTC 
ACCATGCTGG TGCAGAGAGG AGTGGCACCT GCTAAGAAGA GCCCCAAACT GGATTATTAT 
GACCGGGCTC CTCCATACAG AGATTACACT AGGATGCAAT TGTCCAGGAA GGACAGTCAG 
AACAGTTCCC AGCATAGCGT CTCCAGCCAC CGGAGCGCCC ACACCGACTC GCCTGTGCAC 
TCGTCTCTGG CACCCCCTCT CAGTGAGAGC AGCACGCCGC CGGCCCCGTC GCAGCCTTTG 
CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA GACGGGACCA CCCACCGAAA ACCAGACCCC 
TTTAAGATCT GGGCCCAGTC CAGGAGCATG TATGAAAGTA GGCTTCCAGA CTTCCAGGAG 
CAGGACATTT TCCTGTGGAG GAAGGACACA GGCTTCGGCT TTAGGATCCT TGGTGGCAAC 
GAGCCAGGAG AACCTATCTA CATCGGCCAC ATAGTAAAGT ACGGCGCTGC AGATGAGGAC 
GGCCGCCTCC GATCCGGAGA TGAACTGATA TGCGTGGACG GCACGGCGGT GGTGGGCAAG 
TCGCATCAAT TGGTGGTGCA GTTGATGCAG CAGGCTGCCA AGCAGGGCCA TGTTAATCTT 
ACTGTGCGAC GCAAAACCAG CTACACAGTT AAATCAGAAG GAGACGTCCC TCCGTCACCA 
GCGTCCTCAC ACCACAGCAG CACCCAGGCG CCCAGCCTGA CGGAGGACAT GGGGAAGCGC 
ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC ACGGTCAGCT CGGGCAGCGG CTCCACCTCT 
GGCATCGGCA GCGGTGGAGG AGGGGGAGGC GGGGGGGCCG GCGTCACTGG GAATGCTGTG 
GTTTCCGCCT CGACGGTTGC GACCACATCC TCTCAGCCTC CCAACGGCAC CACAAACGCT 
GCCCCCGCCT CGGCCGTTCA GCCCTACGAC GTGGAGATCC GACGGGGCGA GAATGAAGGG 
TTCGGTTTCG TCATCGTGTC ATCAGTGTCG CGGCCTGAAG CTGGCACCAC CTTCGCTGGA 
AATGCATGTG TGGCCATGCC TCACAAAATA GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385274.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240760.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X7, mRNA.

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CloneID	OCy15961
Clone ID Related Accession (Same CDS sequence)	XM_015385273.1 , XM_015385274.1
Accession Version	XM_015385274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CTGGAATAGG GCTTACAGAT AACCAACTGG AGGACGACGA GGACCTCCCT 
GACATGAACA GCAGCTTTAC AGGAGATTCT AGCGAAATAG ACGAGCTTCA TCACTCAGCG 
CGCCCCCACG TCCCTCGCTA TAGTGACCCG TCCTACGCTG GGACAACCCC CTCACCATCG 
GCCACCACGG AGAGCACACA GCAGACAAAC CTTCACCTGA GCCATCCTCC CCCGGAGGAC 
CCGCTGGGAC CTCTGCCTGA CAACTGGGAG ATGGCCTACA CCGAGAATGG AGAGGTCTAC 
TTTATAGACC ACAATACAAA GACCACCTCT TGGTTAGACC CTCGGTGCCT GGACAAACCT 
CAGAAACCCC TGGAAGAATG TGAAGATGAT GAAGGGGTCC ATACAGAAGA GCTGGATGAT 
CTCGAACTTC CTCCAGGATG GGAGAAAATT GATGATCCAG TCTATGGGGT CTACTATGTT 
GATCATATCA ACAGGAAGAC CCAGTATGAA AACCCAGTGC TGGAGGCTAA GAGGCGCAGG 
CAGCTGGAAC AGCAGCCCGC CCAGCAGCCT CAGCCCCAAA GCCAGCAGCC ACCTGAAGGT 
GAGCGCTACA TCCGAGAATG GATCGAGGAC TCCGCATTGG CAGGCGCCCC ACTGGCTACC 
TACACTGCCA ACCACGAGAC CTACAGGGAC CCTCAAACAG GTCCGCCTGT TCCCAACGCC 
ATGGGACAAA AACGTGGGAA GCCTTTCTTT ACCAGGAACC GATCAGAACT GAATGGAACA 
TTCATCAATA CCAAATTGAA GAAGAGCCGT CGGGGATTTG GGTTCACAGT TGTTGGAGGA 
GACGAGCCAG ATGAGTTCTT GCAGATAAAG AGCTTGGTTC TGGACGGACC TGCAGCCCTC 
GATGGCAAGA TGGAGACAGG TGATGTAATC GTGAGTGTAA ACGAAACCTG TGTCCTGGGC 
TACACGCACG CCCAAGTTGT GAAGATCTTT CAGTCCATCC CAATCGGCTC CATGGTTAAC 
TTGGAGCTGT GCCGTGGCTA CCCACTGCCT TTCGACCCTG ACGACCCCAA CACCAGCTTG 
GTAACCTCAG TGGCCATCCT CGACAACAAA GATCCCATCA TCGTCAACGG CCAGGAAGTC 
ACCAACAGCT ACGACTCCCC TTCCAGCCAG GGCAGTCAGA GCACCAACAA CGGCCCGACC 
AACGGCGGGC CACCTCTCAA TGGCTTCACC CGACCTCACA GCCCCTCCAC TGAGGTGGCG 
TCTGATACCT CCTCGCAGCT CGGCTACTCT AGCGACGTGG TGAACCTGGC TTCGGCCATC 
GCCACGCAAC CAGAACTTAT CACTGTACAC ATGGAGAAAG GAGACAAGGG CTTTGGCTTC 
ACCATCGCTG ACAGCCTCGG GGGTGGAGGG CAGAGGGTGA AGCAGATTGT GGACTACCCT 
CGCTGCCGCG GCCTCAGGGA GGGCGACATC ATCGTCGAGG TGAACAAGAG GAATGTGCAG 
AGCATGTCTC ACAACCAGGT GGTGGACATG CTCAGCAAGT TACCAAAAGG CAGCGAGGTC 
ACCATGCTGG TGCAGAGAGG AGTGGCACCT GCTAAGAAGA GCCCCAAACT GGATTATTAT 
GACCGGGCTC CTCCATACAG AGATTACACT AGGATGCAAT TGTCCAGGAA GGACAGTCAG 
AACAGTTCCC AGCATAGCGT CTCCAGCCAC CGGAGCGCCC ACACCGACTC GCCTGTGCAC 
TCGTCTCTGG CACCCCCTCT CAGTGAGAGC AGCACGCCGC CGGCCCCGTC GCAGCCTTTG 
CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA GACGGGACCA CCCACCGAAA ACCAGACCCC 
TTTAAGATCT GGGCCCAGTC CAGGAGCATG TATGAAAGTA GGCTTCCAGA CTTCCAGGAG 
CAGGACATTT TCCTGTGGAG GAAGGACACA GGCTTCGGCT TTAGGATCCT TGGTGGCAAC 
GAGCCAGGAG AACCTATCTA CATCGGCCAC ATAGTAAAGT ACGGCGCTGC AGATGAGGAC 
GGCCGCCTCC GATCCGGAGA TGAACTGATA TGCGTGGACG GCACGGCGGT GGTGGGCAAG 
TCGCATCAAT TGGTGGTGCA GTTGATGCAG CAGGCTGCCA AGCAGGGCCA TGTTAATCTT 
ACTGTGCGAC GCAAAACCAG CTACACAGTT AAATCAGAAG GAGACGTCCC TCCGTCACCA 
GCGTCCTCAC ACCACAGCAG CACCCAGGCG CCCAGCCTGA CGGAGGACAT GGGGAAGCGC 
ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC ACGGTCAGCT CGGGCAGCGG CTCCACCTCT 
GGCATCGGCA GCGGTGGAGG AGGGGGAGGC GGGGGGGCCG GCGTCACTGG GAATGCTGTG 
GTTTCCGCCT CGACGGTTGC GACCACATCC TCTCAGCCTC CCAACGGCAC CACAAACGCT 
GCCCCCGCCT CGGCCGTTCA GCCCTACGAC GTGGAGATCC GACGGGGCGA GAATGAAGGG 
TTCGGTTTCG TCATCGTGTC ATCAGTGTCG CGGCCTGAAG CTGGCACCAC CTTCGCTGGA 
AATGCATGTG TGGCCATGCC TCACAAAATA GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240760.1
CDS	478..4974
Translation	MQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRGKPFFTRNRSELNGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNETCVLGYTHAQVVKIFQSIPIGSMVNLELCRGYPLPFDPDDPNTSLVTSVAILDNKDPIIVNGQEVTNSYDSPSSQGSQSTNNGPTNGGPPLNGFTRPHSPSTEVASDTSSQLGYSSDVVNLASAIATQPELITVHMEKGDKGFGFTIADSLGGGGQRVKQIVDYPRCRGLREGDIIVEVNKRNVQSMSHNQVVDMLSKLPKGSEVTMLVQRGVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385274.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385274.1

ORF Insert Sequence:

ATGCAAAATG CTGGAATAGG GCTTACAGAT AACCAACTGG AGGACGACGA GGACCTCCCT 
GACATGAACA GCAGCTTTAC AGGAGATTCT AGCGAAATAG ACGAGCTTCA TCACTCAGCG 
CGCCCCCACG TCCCTCGCTA TAGTGACCCG TCCTACGCTG GGACAACCCC CTCACCATCG 
GCCACCACGG AGAGCACACA GCAGACAAAC CTTCACCTGA GCCATCCTCC CCCGGAGGAC 
CCGCTGGGAC CTCTGCCTGA CAACTGGGAG ATGGCCTACA CCGAGAATGG AGAGGTCTAC 
TTTATAGACC ACAATACAAA GACCACCTCT TGGTTAGACC CTCGGTGCCT GGACAAACCT 
CAGAAACCCC TGGAAGAATG TGAAGATGAT GAAGGGGTCC ATACAGAAGA GCTGGATGAT 
CTCGAACTTC CTCCAGGATG GGAGAAAATT GATGATCCAG TCTATGGGGT CTACTATGTT 
GATCATATCA ACAGGAAGAC CCAGTATGAA AACCCAGTGC TGGAGGCTAA GAGGCGCAGG 
CAGCTGGAAC AGCAGCCCGC CCAGCAGCCT CAGCCCCAAA GCCAGCAGCC ACCTGAAGGT 
GAGCGCTACA TCCGAGAATG GATCGAGGAC TCCGCATTGG CAGGCGCCCC ACTGGCTACC 
TACACTGCCA ACCACGAGAC CTACAGGGAC CCTCAAACAG GTCCGCCTGT TCCCAACGCC 
ATGGGACAAA AACGTGGGAA GCCTTTCTTT ACCAGGAACC GATCAGAACT GAATGGAACA 
TTCATCAATA CCAAATTGAA GAAGAGCCGT CGGGGATTTG GGTTCACAGT TGTTGGAGGA 
GACGAGCCAG ATGAGTTCTT GCAGATAAAG AGCTTGGTTC TGGACGGACC TGCAGCCCTC 
GATGGCAAGA TGGAGACAGG TGATGTAATC GTGAGTGTAA ACGAAACCTG TGTCCTGGGC 
TACACGCACG CCCAAGTTGT GAAGATCTTT CAGTCCATCC CAATCGGCTC CATGGTTAAC 
TTGGAGCTGT GCCGTGGCTA CCCACTGCCT TTCGACCCTG ACGACCCCAA CACCAGCTTG 
GTAACCTCAG TGGCCATCCT CGACAACAAA GATCCCATCA TCGTCAACGG CCAGGAAGTC 
ACCAACAGCT ACGACTCCCC TTCCAGCCAG GGCAGTCAGA GCACCAACAA CGGCCCGACC 
AACGGCGGGC CACCTCTCAA TGGCTTCACC CGACCTCACA GCCCCTCCAC TGAGGTGGCG 
TCTGATACCT CCTCGCAGCT CGGCTACTCT AGCGACGTGG TGAACCTGGC TTCGGCCATC 
GCCACGCAAC CAGAACTTAT CACTGTACAC ATGGAGAAAG GAGACAAGGG CTTTGGCTTC 
ACCATCGCTG ACAGCCTCGG GGGTGGAGGG CAGAGGGTGA AGCAGATTGT GGACTACCCT 
CGCTGCCGCG GCCTCAGGGA GGGCGACATC ATCGTCGAGG TGAACAAGAG GAATGTGCAG 
AGCATGTCTC ACAACCAGGT GGTGGACATG CTCAGCAAGT TACCAAAAGG CAGCGAGGTC 
ACCATGCTGG TGCAGAGAGG AGTGGCACCT GCTAAGAAGA GCCCCAAACT GGATTATTAT 
GACCGGGCTC CTCCATACAG AGATTACACT AGGATGCAAT TGTCCAGGAA GGACAGTCAG 
AACAGTTCCC AGCATAGCGT CTCCAGCCAC CGGAGCGCCC ACACCGACTC GCCTGTGCAC 
TCGTCTCTGG CACCCCCTCT CAGTGAGAGC AGCACGCCGC CGGCCCCGTC GCAGCCTTTG 
CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA GACGGGACCA CCCACCGAAA ACCAGACCCC 
TTTAAGATCT GGGCCCAGTC CAGGAGCATG TATGAAAGTA GGCTTCCAGA CTTCCAGGAG 
CAGGACATTT TCCTGTGGAG GAAGGACACA GGCTTCGGCT TTAGGATCCT TGGTGGCAAC 
GAGCCAGGAG AACCTATCTA CATCGGCCAC ATAGTAAAGT ACGGCGCTGC AGATGAGGAC 
GGCCGCCTCC GATCCGGAGA TGAACTGATA TGCGTGGACG GCACGGCGGT GGTGGGCAAG 
TCGCATCAAT TGGTGGTGCA GTTGATGCAG CAGGCTGCCA AGCAGGGCCA TGTTAATCTT 
ACTGTGCGAC GCAAAACCAG CTACACAGTT AAATCAGAAG GAGACGTCCC TCCGTCACCA 
GCGTCCTCAC ACCACAGCAG CACCCAGGCG CCCAGCCTGA CGGAGGACAT GGGGAAGCGC 
ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC ACGGTCAGCT CGGGCAGCGG CTCCACCTCT 
GGCATCGGCA GCGGTGGAGG AGGGGGAGGC GGGGGGGCCG GCGTCACTGG GAATGCTGTG 
GTTTCCGCCT CGACGGTTGC GACCACATCC TCTCAGCCTC CCAACGGCAC CACAAACGCT 
GCCCCCGCCT CGGCCGTTCA GCCCTACGAC GTGGAGATCC GACGGGGCGA GAATGAAGGG 
TTCGGTTTCG TCATCGTGTC ATCAGTGTCG CGGCCTGAAG CTGGCACCAC CTTCGCTGGA 
AATGCATGTG TGGCCATGCC TCACAAAATA GGCCGCATCA TCGAGGGGAG TCCAGCGGAC 
CGCTGCGGGA AGCTGAAGGT GGGCGACCGC ATCCTGGCTG TCAACGGGAA CTCCATCACC 
AACAAGTCCC ACTCTGACAT CGTCAACCTG ATCAAGGAGG CTGGGAACAC CGTCACGCTG 
CGGATCATTC CTGGAGATGA GTCATCCAAT GCATCTTTGT TGACAAATGC AGAGAAGATT 
GCCACCATAA CCACCACTCA CACAGCTCAG CAACAGGCAG CACCTGAGGC CAGGACCAAC 
ACTAAACCAA AACAGGAATC TTATGAGTTT AAAGCTCCTA CGAACACTGC TCCCCAGCCC 
CCCATCCAGC AAGACTCAGC TCAGGACTCC GAGTTCTACT CTGTGGACCT GGAGAGGGAC 
AACAAAGGCT TTGGCTTCAG CCTGCGGGGA GGAAGAGAGT ACAACATGGA CCTGTACGTG 
CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT CGCAATGGGA AAATGAGGGT TGGAGATGAG 
ATCTTGGAGA TCAACGGCGA AAGCACCAAA GGCATGAAGC ACGCACGGGC CATCGAGCTC 
ATCAAGAACG GAGGCCGAAA CGTCCACCTG GTGCTCAAGA GGGGTGACGG CTCCGTGCCT 
GAATATGACG GCAGCCCCAA CGACATCAGC ACAGTTGACC CAGTCACAAG GATACAAAAC 
GCTGCGGAAG TGAGTTCCCT GCCTCTAACT AATGCACCAT CAGAGTCCAG CTTCCCACCA 
GAACCCCCAC CTCATTCACG GAGCAAAACG GAGCCGAGCC GTCGCACCCA TGGCACTGGC 
AAGCCCCATC GTTCCCATCA TCGTCATCAC TCCCCTAGCA AATCCAAAGG GAAGAAGTCT 
AAAGGAAGGC ATGGACACAA GCAGAATAAG AAAAAGGATG ACAAGGAAAA AGAAGGCCAT 
CGGCATCGAA GCCGCCACCG CTCGCCTCAT AGGGGGCAGG GCCGTTCACG TAGTGCAGAC 
AACATGCTGG ATAGCCGTGA TAGAGGGCAC CGCCGAGGCC GTTCACCAGA ACGGCGCCAC 
GGCCAACACT CCTCGAACCA CCGCCACGGC TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT 
CCAACCCGCT CACCATACCG ACGCTCCCCA TACCGCTCTC CTAGACGCTC CCCTTACCGC 
TCTCATCGCC ACTCTCCTCG ACGTTCTCCT CATCGCCACA GGTCCCCCAA CCGCTACCAC 
TCTCCAAACA GACATCGCCA CCGCTCTGCA GACCCCTACC GTTATACCGC CACTGAGAGA 
AACAATGAAA AGCCAACGGT AGATGAGGCT GCAATGACCG GGACTCCAGA ACCAAGCCTC 
CCTTTTGAAG AGAGTATCCT TCGGGAGTCG CCGGCCCTGG ACCGCCTGAA CAGGGACCCC 
ACTCTTTCAC TTCCACCACT ACAGAGAGAG GATCGTCTCT ACGGGCAGGA CAGTCTTCTA 
ATGAGAACCT CCTCCATGGA AAGAGCCTAC AGAGGAGACA GACTTGGCAT GGCATCCCGT 
GACAACTTCA AAGACGTCAC CCTGCCCAGA GTCCGCACCC CTGATTTTGA TTCATCCTAC 
CAGAAGTACA CCTCCCTGTT GAGAGGGCGT ACTCCTGAGA TGGTTGAGAG GGATGGGCTC 
TTTGCCAGTC GAGGTAGCAC CCCTGAACCC CGATACCGTG CTCGCAGCCT TGGACGGGCC 
ATCTCCCCAG CCAGGAGGGA CGACTCGGAG GATGAAGAAG ATGACGATCA TTTTGTTGCG 
GCTAAGGTGA GAGAGTACTA CAGCACACTC AAGTCAGACA CCAGCCGCCA AACCAAGCCT 
ATGATCCCTG AGCCTAAGAA GACCTACAAG GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385275.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240761.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X8, mRNA.

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CloneID	OCy15962
Clone ID Related Accession (Same CDS sequence)	XM_015385275.1
Accession Version	XM_015385275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG AGTGGCACCT 
GCTAAGAAGA GCCCCAAACT GGATTATTAT GACCGGGCTC CTCCATACAG AGATTACACT 
AGGATGCAAT TGTCCAGGAA GGACAGTCAG AACAGTTCCC AGCATAGCGT CTCCAGCCAC 
CGGAGCGCCC ACACCGACTC GCCTGTGCAC TCGTCTCTGG CACCCCCTCT CAGTGAGAGC 
AGCACGCCGC CGGCCCCGTC GCAGCCTTTG CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA 
GACGGGACCA CCCACCGAAA ACCAGACCCC TTTAAGATCT GGGCCCAGTC CAGGAGCATG 
TATGAAAGTA GGCTTCCAGA CTTCCAGGAG CAGGACATTT TCCTGTGGAG GAAGGACACA 
GGCTTCGGCT TTAGGATCCT TGGTGGCAAC GAGCCAGGAG AACCTATCTA CATCGGCCAC 
ATAGTAAAGT ACGGCGCTGC AGATGAGGAC GGCCGCCTCC GATCCGGAGA TGAACTGATA 
TGCGTGGACG GCACGGCGGT GGTGGGCAAG TCGCATCAAT TGGTGGTGCA GTTGATGCAG 
CAGGCTGCCA AGCAGGGCCA TGTTAATCTT ACTGTGCGAC GCAAAACCAG CTACACAGTT 
AAATCAGAAG GAGACGTCCC TCCGTCACCA GCGTCCTCAC ACCACAGCAG CACCCAGGCG 
CCCAGCCTGA CGGAGGACAT GGGGAAGCGC ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC 
ACGGTCAGCT CGGGCAGCGG CTCCACCTCT GGCATCGGCA GCGGTGGAGG AGGGGGAGGC 
GGGGGGGCCG GCGTCACTGG GAATGCTGTG GTTTCCGCCT CGACGGTTGC GACCACATCC 
TCTCAGCCTC CCAACGGCAC CACAAACGCT GCCCCCGCCT CGGCCGTTCA GCCCTACGAC 
GTGGAGATCC GACGGGGCGA GAATGAAGGG TTCGGTTTCG TCATCGTGTC ATCAGTGTCG 
CGGCCTGAAG CTGGCACCAC CTTCGCTGGA AATGCATGTG TGGCCATGCC TCACAAAATA 
GGCCGCATCA TCGAGGGGAG TCCAGCGGAC CGCTGCGGGA AGCTGAAGGT GGGCGACCGC 
ATCCTGGCTG TCAACGGGAA CTCCATCACC AACAAGTCCC ACTCTGACAT CGTCAACCTG 
ATCAAGGAGG CTGGGAACAC CGTCACGCTG CGGATCATTC CTGGAGATGA GTCATCCAAT 
GCATCTTTGT TGACAAATGC AGAGAAGATT GCCACCATAA CCACCACTCA CACAGCTCAG 
CAACAGGCAG CACCTGAGGC CAGGACCAAC ACTAAACCAA AACAGGAATC TTATGAGTTT 
AAAGCTCCTA CGAACACTGC TCCCCAGCCC CCCATCCAGC AAGACTCAGC TCAGGACTCC 
GAGTTCTACT CTGTGGACCT GGAGAGGGAC AACAAAGGCT TTGGCTTCAG CCTGCGGGGA 
GGAAGAGAGT ACAACATGGA CCTGTACGTG CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT 
CGCAATGGGA AAATGAGGGT TGGAGATGAG ATCTTGGAGA TCAACGGCGA AAGCACCAAA 
GGCATGAAGC ACGCACGGGC CATCGAGCTC ATCAAGAACG GAGGCCGAAA CGTCCACCTG 
GTGCTCAAGA GGGGTGACGG CTCCGTGCCT GAATATGACG GCAGCCCCAA CGACATCAGC 
ACAGTTGACC CAGTCACAAG GATACAAAAC GCTGCGGAAG TGAGTTCCCT GCCTCTAACT 
AATGCACCAT CAGAGTCCAG CTTCCCACCA GAACCCCCAC CTCATTCACG GAGCAAAACG 
GAGCCGAGCC GTCGCACCCA TGGCACTGGC AAGCCCCATC GTTCCCATCA TCGTCATCAC 
TCCCCTAGCA AATCCAAAGG GAAGAAGTCT AAAGGAAGGC ATGGACACAA GCAGAATAAG 
AAAAAGGATG ACAAGGAAAA AGAAGGCCAT CGGCATCGAA GCCGCCACCG CTCGCCTCAT 
AGGGGGCAGG GCCGTTCACG TAGTGCAGAC AACATGCTGG ATAGCCGTGA TAGAGGGCAC 
CGCCGAGGCC GTTCACCAGA ACGGCGCCAC GGCCAACACT CCTCGAACCA CCGCCACGGC 
TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT CCAACCCGCT CACCATACCG ACGCTCCCCA 
TACCGCTCTC CTAGACGCTC CCCTTACCGC TCTCATCGCC ACTCTCCTCG ACGTTCTCCT 
CATCGCCACA GGTCCCCCAA CCGCTACCAC TCTCCAAACA GACATCGCCA CCGCTCTGCA 
GACCCCTACC GTTATACCGC CACTGAGAGA AACAATGAAA AGCCAACGGT AGATGAGGCT 
GCAATGACCG GGACTCCAGA ACCAAGCCTC CCTTTTGAAG AGAGTATCCT TCGGGAGTCG 
CCGGCCCTGG ACCGCCTGAA CAGGGACCCC ACTCTTTCAC TTCCACCACT ACAGAGAGAG 
GATCGTCTCT ACGGGCAGGA CAGTCTTCTA ATGAGAACCT CCTCCATGGA AAGAGCCTAC 
AGAGGAGACA GACTTGGCAT GGCATCCCGT GACAACTTCA AAGACGTCAC CCTGCCCAGA 
GTCCGCACCC CTGATTTTGA TTCATCCTAC CAGAAGTACA CCTCCCTGTT GAGAGGGCGT 
ACTCCTGAGA TGGTTGAGAG GGATGGGCTC TTTGCCAGTC GAGGTAGCAC CCCTGAACCC 
CGATACCGTG CTCGCAGCCT TGGACGGGCC ATCTCCCCAG CCAGGAGGGA CGACTCGGAG 
GATGAAGAAG ATGACGATCA TTTTGTTGCG GCTAAGGTGA GAGAGTACTA CAGCACACTC 
AAGTCAGACA CCAGCCGCCA AACCAAGCCT ATGATCCCTG AGCCTAAGAA GACCTACAAG 
GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240761.1
CDS	388..4734
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385275.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385275.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG AGTGGCACCT 
GCTAAGAAGA GCCCCAAACT GGATTATTAT GACCGGGCTC CTCCATACAG AGATTACACT 
AGGATGCAAT TGTCCAGGAA GGACAGTCAG AACAGTTCCC AGCATAGCGT CTCCAGCCAC 
CGGAGCGCCC ACACCGACTC GCCTGTGCAC TCGTCTCTGG CACCCCCTCT CAGTGAGAGC 
AGCACGCCGC CGGCCCCGTC GCAGCCTTTG CCTGGCCTGC CTTCCCAGGA CTCTCAAGGA 
GACGGGACCA CCCACCGAAA ACCAGACCCC TTTAAGATCT GGGCCCAGTC CAGGAGCATG 
TATGAAAGTA GGCTTCCAGA CTTCCAGGAG CAGGACATTT TCCTGTGGAG GAAGGACACA 
GGCTTCGGCT TTAGGATCCT TGGTGGCAAC GAGCCAGGAG AACCTATCTA CATCGGCCAC 
ATAGTAAAGT ACGGCGCTGC AGATGAGGAC GGCCGCCTCC GATCCGGAGA TGAACTGATA 
TGCGTGGACG GCACGGCGGT GGTGGGCAAG TCGCATCAAT TGGTGGTGCA GTTGATGCAG 
CAGGCTGCCA AGCAGGGCCA TGTTAATCTT ACTGTGCGAC GCAAAACCAG CTACACAGTT 
AAATCAGAAG GAGACGTCCC TCCGTCACCA GCGTCCTCAC ACCACAGCAG CACCCAGGCG 
CCCAGCCTGA CGGAGGACAT GGGGAAGCGC ACCCCGCAAG GCAGCCAGAA TTCCCTCAAC 
ACGGTCAGCT CGGGCAGCGG CTCCACCTCT GGCATCGGCA GCGGTGGAGG AGGGGGAGGC 
GGGGGGGCCG GCGTCACTGG GAATGCTGTG GTTTCCGCCT CGACGGTTGC GACCACATCC 
TCTCAGCCTC CCAACGGCAC CACAAACGCT GCCCCCGCCT CGGCCGTTCA GCCCTACGAC 
GTGGAGATCC GACGGGGCGA GAATGAAGGG TTCGGTTTCG TCATCGTGTC ATCAGTGTCG 
CGGCCTGAAG CTGGCACCAC CTTCGCTGGA AATGCATGTG TGGCCATGCC TCACAAAATA 
GGCCGCATCA TCGAGGGGAG TCCAGCGGAC CGCTGCGGGA AGCTGAAGGT GGGCGACCGC 
ATCCTGGCTG TCAACGGGAA CTCCATCACC AACAAGTCCC ACTCTGACAT CGTCAACCTG 
ATCAAGGAGG CTGGGAACAC CGTCACGCTG CGGATCATTC CTGGAGATGA GTCATCCAAT 
GCATCTTTGT TGACAAATGC AGAGAAGATT GCCACCATAA CCACCACTCA CACAGCTCAG 
CAACAGGCAG CACCTGAGGC CAGGACCAAC ACTAAACCAA AACAGGAATC TTATGAGTTT 
AAAGCTCCTA CGAACACTGC TCCCCAGCCC CCCATCCAGC AAGACTCAGC TCAGGACTCC 
GAGTTCTACT CTGTGGACCT GGAGAGGGAC AACAAAGGCT TTGGCTTCAG CCTGCGGGGA 
GGAAGAGAGT ACAACATGGA CCTGTACGTG CTGAGGCTTG CTGAAGAAGG AGCTGCTGTT 
CGCAATGGGA AAATGAGGGT TGGAGATGAG ATCTTGGAGA TCAACGGCGA AAGCACCAAA 
GGCATGAAGC ACGCACGGGC CATCGAGCTC ATCAAGAACG GAGGCCGAAA CGTCCACCTG 
GTGCTCAAGA GGGGTGACGG CTCCGTGCCT GAATATGACG GCAGCCCCAA CGACATCAGC 
ACAGTTGACC CAGTCACAAG GATACAAAAC GCTGCGGAAG TGAGTTCCCT GCCTCTAACT 
AATGCACCAT CAGAGTCCAG CTTCCCACCA GAACCCCCAC CTCATTCACG GAGCAAAACG 
GAGCCGAGCC GTCGCACCCA TGGCACTGGC AAGCCCCATC GTTCCCATCA TCGTCATCAC 
TCCCCTAGCA AATCCAAAGG GAAGAAGTCT AAAGGAAGGC ATGGACACAA GCAGAATAAG 
AAAAAGGATG ACAAGGAAAA AGAAGGCCAT CGGCATCGAA GCCGCCACCG CTCGCCTCAT 
AGGGGGCAGG GCCGTTCACG TAGTGCAGAC AACATGCTGG ATAGCCGTGA TAGAGGGCAC 
CGCCGAGGCC GTTCACCAGA ACGGCGCCAC GGCCAACACT CCTCGAACCA CCGCCACGGC 
TCTCCTCGAC GCTCCCCTTA TCGCCGCTCT CCAACCCGCT CACCATACCG ACGCTCCCCA 
TACCGCTCTC CTAGACGCTC CCCTTACCGC TCTCATCGCC ACTCTCCTCG ACGTTCTCCT 
CATCGCCACA GGTCCCCCAA CCGCTACCAC TCTCCAAACA GACATCGCCA CCGCTCTGCA 
GACCCCTACC GTTATACCGC CACTGAGAGA AACAATGAAA AGCCAACGGT AGATGAGGCT 
GCAATGACCG GGACTCCAGA ACCAAGCCTC CCTTTTGAAG AGAGTATCCT TCGGGAGTCG 
CCGGCCCTGG ACCGCCTGAA CAGGGACCCC ACTCTTTCAC TTCCACCACT ACAGAGAGAG 
GATCGTCTCT ACGGGCAGGA CAGTCTTCTA ATGAGAACCT CCTCCATGGA AAGAGCCTAC 
AGAGGAGACA GACTTGGCAT GGCATCCCGT GACAACTTCA AAGACGTCAC CCTGCCCAGA 
GTCCGCACCC CTGATTTTGA TTCATCCTAC CAGAAGTACA CCTCCCTGTT GAGAGGGCGT 
ACTCCTGAGA TGGTTGAGAG GGATGGGCTC TTTGCCAGTC GAGGTAGCAC CCCTGAACCC 
CGATACCGTG CTCGCAGCCT TGGACGGGCC ATCTCCCCAG CCAGGAGGGA CGACTCGGAG 
GATGAAGAAG ATGACGATCA TTTTGTTGCG GCTAAGGTGA GAGAGTACTA CAGCACACTC 
AAGTCAGACA CCAGCCGCCA AACCAAGCCT ATGATCCCTG AGCCTAAGAA GACCTACAAG 
GATAATCCCA AAGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385276.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240762.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCy15963
Clone ID Related Accession (Same CDS sequence)	XM_015385276.1
Accession Version	XM_015385276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGAATGGA TCGAGGACTC CGCATTGGCA 
GGCGCCCCAC TGGCTACCTA CACTGCCAAC CACGAGACCT ACAGGGACCC TCAAACAGGT 
CCGCCTGTTC CCAACGCCAT GGGACAAAAA CGAGTGGCAC CTGCTAAGAA GAGCCCCAAA 
CTGGATTATT ATGACCGGGC TCCTCCATAC AGAGATTACA CTAGGATGCA ATTGTCCAGG 
AAGGACAGTC AGAACAGTTC CCAGCATAGC GTCTCCAGCC ACCGGAGCGC CCACACCGAC 
TCGCCTGTGC ACTCGTCTCT GGCACCCCCT CTCAGTGAGA GCAGCACGCC GCCGGCCCCG 
TCGCAGCCTT TGCCTGGCCT GCCTTCCCAG GACTCTCAAG GAGACGGGAC CACCCACCGA 
AAACCAGACC CCTTTAAGAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTTCCA 
GACTTCCAGG AGCAGGACAT TTTCCTGTGG AGGAAGGACA CAGGCTTCGG CTTTAGGATC 
CTTGGTGGCA ACGAGCCAGG AGAACCTATC TACATCGGCC ACATAGTAAA GTACGGCGCT 
GCAGATGAGG ACGGCCGCCT CCGATCCGGA GATGAACTGA TATGCGTGGA CGGCACGGCG 
GTGGTGGGCA AGTCGCATCA ATTGGTGGTG CAGTTGATGC AGCAGGCTGC CAAGCAGGGC 
CATGTTAATC TTACTGTGCG ACGCAAAACC AGCTACACAG TTAAATCAGA AGGAGACGTC 
CCTCCGTCAC CAGCGTCCTC ACACCACAGC AGCACCCAGG CGCCCAGCCT GACGGAGGAC 
ATGGGGAAGC GCACCCCGCA AGGCAGCCAG AATTCCCTCA ACACGGTCAG CTCGGGCAGC 
GGCTCCACCT CTGGCATCGG CAGCGGTGGA GGAGGGGGAG GCGGGGGGGC CGGCGTCACT 
GGGAATGCTG TGGTTTCCGC CTCGACGGTT GCGACCACAT CCTCTCAGCC TCCCAACGGC 
ACCACAAACG CTGCCCCCGC CTCGGCCGTT CAGCCCTACG ACGTGGAGAT CCGACGGGGC 
GAGAATGAAG GGTTCGGTTT CGTCATCGTG TCATCAGTGT CGCGGCCTGA AGCTGGCACC 
ACCTTCGCTG GAAATGCATG TGTGGCCATG CCTCACAAAA TAGGCCGCAT CATCGAGGGG 
AGTCCAGCGG ACCGCTGCGG GAAGCTGAAG GTGGGCGACC GCATCCTGGC TGTCAACGGG 
AACTCCATCA CCAACAAGTC CCACTCTGAC ATCGTCAACC TGATCAAGGA GGCTGGGAAC 
ACCGTCACGC TGCGGATCAT TCCTGGAGAT GAGTCATCCA ATGCATCTTT GTTGACAAAT 
GCAGAGAAGA TTGCCACCAT AACCACCACT CACACAGCTC AGCAACAGGC AGCACCTGAG 
GCCAGGACCA ACACTAAACC AAAACAGGAA TCTTATGAGT TTAAAGCTCC TACGAACACT 
GCTCCCCAGC CCCCCATCCA GCAAGACTCA GCTCAGGACT CCGAGTTCTA CTCTGTGGAC 
CTGGAGAGGG ACAACAAAGG CTTTGGCTTC AGCCTGCGGG GAGGAAGAGA GTACAACATG 
GACCTGTACG TGCTGAGGCT TGCTGAAGAA GGAGCTGCTG TTCGCAATGG GAAAATGAGG 
GTTGGAGATG AGATCTTGGA GATCAACGGC GAAAGCACCA AAGGCATGAA GCACGCACGG 
GCCATCGAGC TCATCAAGAA CGGAGGCCGA AACGTCCACC TGGTGCTCAA GAGGGGTGAC 
GGCTCCGTGC CTGAATATGA CGGCAGCCCC AACGACATCA GCACAGTTGA CCCAGTCACA 
AGGATACAAA ACGCTGCGGA AGTGAGTTCC CTGCCTCTAA CTAATGCACC ATCAGAGTCC 
AGCTTCCCAC CAGAACCCCC ACCTCATTCA CGGAGCAAAA CGGAGCCGAG CCGTCGCACC 
CATGGCACTG GCAAGCCCCA TCGTTCCCAT CATCGTCATC ACTCCCCTAG CAAATCCAAA 
GGGAAGAAGT CTAAAGGAAG GCATGGACAC AAGCAGAATA AGAAAAAGGA TGACAAGGAA 
AAAGAAGGCC ATCGGCATCG AAGCCGCCAC CGCTCGCCTC ATAGGGGGCA GGGCCGTTCA 
CGTAGTGCAG ACAACATGCT GGATAGCCGT GATAGAGGGC ACCGCCGAGG CCGTTCACCA 
GAACGGCGCC ACGGCCAACA CTCCTCGAAC CACCGCCACG GCTCTCCTCG ACGCTCCCCT 
TATCGCCGCT CTCCAACCCG CTCACCATAC CGACGCTCCC CATACCGCTC TCCTAGACGC 
TCCCCTTACC GCTCTCATCG CCACTCTCCT CGACGTTCTC CTCATCGCCA CAGGTCCCCC 
AACCGCTACC ACTCTCCAAA CAGACATCGC CACCGCTCTG CAGACCCCTA CCGTTATACC 
GCCACTGAGA GAAACAATGA AAAGCCAACG GTAGATGAGG CTGCAATGAC CGGGACTCCA 
GAACCAAGCC TCCCTTTTGA AGAGAGTATC CTTCGGGAGT CGCCGGCCCT GGACCGCCTG 
AACAGGGACC CCACTCTTTC ACTTCCACCA CTACAGAGAG AGGATCGTCT CTACGGGCAG 
GACAGTCTTC TAATGAGAAC CTCCTCCATG GAAAGAGCCT ACAGAGGAGA CAGACTTGGC 
ATGGCATCCC GTGACAACTT CAAAGACGTC ACCCTGCCCA GAGTCCGCAC CCCTGATTTT 
GATTCATCCT ACCAGAAGTA CACCTCCCTG TTGAGAGGGC GTACTCCTGA GATGGTTGAG 
AGGGATGGGC TCTTTGCCAG TCGAGGTAGC ACCCCTGAAC CCCGATACCG TGCTCGCAGC 
CTTGGACGGG CCATCTCCCC AGCCAGGAGG GACGACTCGG AGGATGAAGA AGATGACGAT 
CATTTTGTTG CGGCTAAGGT GAGAGAGTAC TACAGCACAC TCAAGTCAGA CACCAGCCGC 
CAAACCAAGC CTATGATCCC TGAGCCTAAG AAGACCTACA AGGATAATCC CAAAGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240762.1
CDS	388..4716
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEEWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRVAPAKKSPKLDYYDRAPPYRDYTRMQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385276.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385276.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGAATGGA TCGAGGACTC CGCATTGGCA 
GGCGCCCCAC TGGCTACCTA CACTGCCAAC CACGAGACCT ACAGGGACCC TCAAACAGGT 
CCGCCTGTTC CCAACGCCAT GGGACAAAAA CGAGTGGCAC CTGCTAAGAA GAGCCCCAAA 
CTGGATTATT ATGACCGGGC TCCTCCATAC AGAGATTACA CTAGGATGCA ATTGTCCAGG 
AAGGACAGTC AGAACAGTTC CCAGCATAGC GTCTCCAGCC ACCGGAGCGC CCACACCGAC 
TCGCCTGTGC ACTCGTCTCT GGCACCCCCT CTCAGTGAGA GCAGCACGCC GCCGGCCCCG 
TCGCAGCCTT TGCCTGGCCT GCCTTCCCAG GACTCTCAAG GAGACGGGAC CACCCACCGA 
AAACCAGACC CCTTTAAGAT CTGGGCCCAG TCCAGGAGCA TGTATGAAAG TAGGCTTCCA 
GACTTCCAGG AGCAGGACAT TTTCCTGTGG AGGAAGGACA CAGGCTTCGG CTTTAGGATC 
CTTGGTGGCA ACGAGCCAGG AGAACCTATC TACATCGGCC ACATAGTAAA GTACGGCGCT 
GCAGATGAGG ACGGCCGCCT CCGATCCGGA GATGAACTGA TATGCGTGGA CGGCACGGCG 
GTGGTGGGCA AGTCGCATCA ATTGGTGGTG CAGTTGATGC AGCAGGCTGC CAAGCAGGGC 
CATGTTAATC TTACTGTGCG ACGCAAAACC AGCTACACAG TTAAATCAGA AGGAGACGTC 
CCTCCGTCAC CAGCGTCCTC ACACCACAGC AGCACCCAGG CGCCCAGCCT GACGGAGGAC 
ATGGGGAAGC GCACCCCGCA AGGCAGCCAG AATTCCCTCA ACACGGTCAG CTCGGGCAGC 
GGCTCCACCT CTGGCATCGG CAGCGGTGGA GGAGGGGGAG GCGGGGGGGC CGGCGTCACT 
GGGAATGCTG TGGTTTCCGC CTCGACGGTT GCGACCACAT CCTCTCAGCC TCCCAACGGC 
ACCACAAACG CTGCCCCCGC CTCGGCCGTT CAGCCCTACG ACGTGGAGAT CCGACGGGGC 
GAGAATGAAG GGTTCGGTTT CGTCATCGTG TCATCAGTGT CGCGGCCTGA AGCTGGCACC 
ACCTTCGCTG GAAATGCATG TGTGGCCATG CCTCACAAAA TAGGCCGCAT CATCGAGGGG 
AGTCCAGCGG ACCGCTGCGG GAAGCTGAAG GTGGGCGACC GCATCCTGGC TGTCAACGGG 
AACTCCATCA CCAACAAGTC CCACTCTGAC ATCGTCAACC TGATCAAGGA GGCTGGGAAC 
ACCGTCACGC TGCGGATCAT TCCTGGAGAT GAGTCATCCA ATGCATCTTT GTTGACAAAT 
GCAGAGAAGA TTGCCACCAT AACCACCACT CACACAGCTC AGCAACAGGC AGCACCTGAG 
GCCAGGACCA ACACTAAACC AAAACAGGAA TCTTATGAGT TTAAAGCTCC TACGAACACT 
GCTCCCCAGC CCCCCATCCA GCAAGACTCA GCTCAGGACT CCGAGTTCTA CTCTGTGGAC 
CTGGAGAGGG ACAACAAAGG CTTTGGCTTC AGCCTGCGGG GAGGAAGAGA GTACAACATG 
GACCTGTACG TGCTGAGGCT TGCTGAAGAA GGAGCTGCTG TTCGCAATGG GAAAATGAGG 
GTTGGAGATG AGATCTTGGA GATCAACGGC GAAAGCACCA AAGGCATGAA GCACGCACGG 
GCCATCGAGC TCATCAAGAA CGGAGGCCGA AACGTCCACC TGGTGCTCAA GAGGGGTGAC 
GGCTCCGTGC CTGAATATGA CGGCAGCCCC AACGACATCA GCACAGTTGA CCCAGTCACA 
AGGATACAAA ACGCTGCGGA AGTGAGTTCC CTGCCTCTAA CTAATGCACC ATCAGAGTCC 
AGCTTCCCAC CAGAACCCCC ACCTCATTCA CGGAGCAAAA CGGAGCCGAG CCGTCGCACC 
CATGGCACTG GCAAGCCCCA TCGTTCCCAT CATCGTCATC ACTCCCCTAG CAAATCCAAA 
GGGAAGAAGT CTAAAGGAAG GCATGGACAC AAGCAGAATA AGAAAAAGGA TGACAAGGAA 
AAAGAAGGCC ATCGGCATCG AAGCCGCCAC CGCTCGCCTC ATAGGGGGCA GGGCCGTTCA 
CGTAGTGCAG ACAACATGCT GGATAGCCGT GATAGAGGGC ACCGCCGAGG CCGTTCACCA 
GAACGGCGCC ACGGCCAACA CTCCTCGAAC CACCGCCACG GCTCTCCTCG ACGCTCCCCT 
TATCGCCGCT CTCCAACCCG CTCACCATAC CGACGCTCCC CATACCGCTC TCCTAGACGC 
TCCCCTTACC GCTCTCATCG CCACTCTCCT CGACGTTCTC CTCATCGCCA CAGGTCCCCC 
AACCGCTACC ACTCTCCAAA CAGACATCGC CACCGCTCTG CAGACCCCTA CCGTTATACC 
GCCACTGAGA GAAACAATGA AAAGCCAACG GTAGATGAGG CTGCAATGAC CGGGACTCCA 
GAACCAAGCC TCCCTTTTGA AGAGAGTATC CTTCGGGAGT CGCCGGCCCT GGACCGCCTG 
AACAGGGACC CCACTCTTTC ACTTCCACCA CTACAGAGAG AGGATCGTCT CTACGGGCAG 
GACAGTCTTC TAATGAGAAC CTCCTCCATG GAAAGAGCCT ACAGAGGAGA CAGACTTGGC 
ATGGCATCCC GTGACAACTT CAAAGACGTC ACCCTGCCCA GAGTCCGCAC CCCTGATTTT 
GATTCATCCT ACCAGAAGTA CACCTCCCTG TTGAGAGGGC GTACTCCTGA GATGGTTGAG 
AGGGATGGGC TCTTTGCCAG TCGAGGTAGC ACCCCTGAAC CCCGATACCG TGCTCGCAGC 
CTTGGACGGG CCATCTCCCC AGCCAGGAGG GACGACTCGG AGGATGAAGA AGATGACGAT 
CATTTTGTTG CGGCTAAGGT GAGAGAGTAC TACAGCACAC TCAAGTCAGA CACCAGCCGC 
CAAACCAAGC CTATGATCCC TGAGCCTAAG AAGACCTACA AGGATAATCC CAAAGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107091395 ( XM_015385277.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015240763.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X10, mRNA.

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CloneID	OCy15964
Clone ID Related Accession (Same CDS sequence)	XM_015385277.1
Accession Version	XM_015385277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150788.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG AGTGGCACCT 
GCTAAGAAGA GCCCCAAACT GCAATTGTCC AGGAAGGACA GTCAGAACAG TTCCCAGCAT 
AGCGTCTCCA GCCACCGGAG CGCCCACACC GACTCGCCTG TGCACTCGTC TCTGGCACCC 
CCTCTCAGTG AGAGCAGCAC GCCGCCGGCC CCGTCGCAGC CTTTGCCTGG CCTGCCTTCC 
CAGGACTCTC AAGGAGACGG GACCACCCAC CGAAAACCAG ACCCCTTTAA GATCTGGGCC 
CAGTCCAGGA GCATGTATGA AAGTAGGCTT CCAGACTTCC AGGAGCAGGA CATTTTCCTG 
TGGAGGAAGG ACACAGGCTT CGGCTTTAGG ATCCTTGGTG GCAACGAGCC AGGAGAACCT 
ATCTACATCG GCCACATAGT AAAGTACGGC GCTGCAGATG AGGACGGCCG CCTCCGATCC 
GGAGATGAAC TGATATGCGT GGACGGCACG GCGGTGGTGG GCAAGTCGCA TCAATTGGTG 
GTGCAGTTGA TGCAGCAGGC TGCCAAGCAG GGCCATGTTA ATCTTACTGT GCGACGCAAA 
ACCAGCTACA CAGTTAAATC AGAAGGAGAC GTCCCTCCGT CACCAGCGTC CTCACACCAC 
AGCAGCACCC AGGCGCCCAG CCTGACGGAG GACATGGGGA AGCGCACCCC GCAAGGCAGC 
CAGAATTCCC TCAACACGGT CAGCTCGGGC AGCGGCTCCA CCTCTGGCAT CGGCAGCGGT 
GGAGGAGGGG GAGGCGGGGG GGCCGGCGTC ACTGGGAATG CTGTGGTTTC CGCCTCGACG 
GTTGCGACCA CATCCTCTCA GCCTCCCAAC GGCACCACAA ACGCTGCCCC CGCCTCGGCC 
GTTCAGCCCT ACGACGTGGA GATCCGACGG GGCGAGAATG AAGGGTTCGG TTTCGTCATC 
GTGTCATCAG TGTCGCGGCC TGAAGCTGGC ACCACCTTCG CTGGAAATGC ATGTGTGGCC 
ATGCCTCACA AAATAGGCCG CATCATCGAG GGGAGTCCAG CGGACCGCTG CGGGAAGCTG 
AAGGTGGGCG ACCGCATCCT GGCTGTCAAC GGGAACTCCA TCACCAACAA GTCCCACTCT 
GACATCGTCA ACCTGATCAA GGAGGCTGGG AACACCGTCA CGCTGCGGAT CATTCCTGGA 
GATGAGTCAT CCAATGCATC TTTGTTGACA AATGCAGAGA AGATTGCCAC CATAACCACC 
ACTCACACAG CTCAGCAACA GGCAGCACCT GAGGCCAGGA CCAACACTAA ACCAAAACAG 
GAATCTTATG AGTTTAAAGC TCCTACGAAC ACTGCTCCCC AGCCCCCCAT CCAGCAAGAC 
TCAGCTCAGG ACTCCGAGTT CTACTCTGTG GACCTGGAGA GGGACAACAA AGGCTTTGGC 
TTCAGCCTGC GGGGAGGAAG AGAGTACAAC ATGGACCTGT ACGTGCTGAG GCTTGCTGAA 
GAAGGAGCTG CTGTTCGCAA TGGGAAAATG AGGGTTGGAG ATGAGATCTT GGAGATCAAC 
GGCGAAAGCA CCAAAGGCAT GAAGCACGCA CGGGCCATCG AGCTCATCAA GAACGGAGGC 
CGAAACGTCC ACCTGGTGCT CAAGAGGGGT GACGGCTCCG TGCCTGAATA TGACGGCAGC 
CCCAACGACA TCAGCACAGT TGACCCAGTC ACAAGGATAC AAAACGCTGC GGAAGTGAGT 
TCCCTGCCTC TAACTAATGC ACCATCAGAG TCCAGCTTCC CACCAGAACC CCCACCTCAT 
TCACGGAGCA AAACGGAGCC GAGCCGTCGC ACCCATGGCA CTGGCAAGCC CCATCGTTCC 
CATCATCGTC ATCACTCCCC TAGCAAATCC AAAGGGAAGA AGTCTAAAGG AAGGCATGGA 
CACAAGCAGA ATAAGAAAAA GGATGACAAG GAAAAAGAAG GCCATCGGCA TCGAAGCCGC 
CACCGCTCGC CTCATAGGGG GCAGGGCCGT TCACGTAGTG CAGACAACAT GCTGGATAGC 
CGTGATAGAG GGCACCGCCG AGGCCGTTCA CCAGAACGGC GCCACGGCCA ACACTCCTCG 
AACCACCGCC ACGGCTCTCC TCGACGCTCC CCTTATCGCC GCTCTCCAAC CCGCTCACCA 
TACCGACGCT CCCCATACCG CTCTCCTAGA CGCTCCCCTT ACCGCTCTCA TCGCCACTCT 
CCTCGACGTT CTCCTCATCG CCACAGGTCC CCCAACCGCT ACCACTCTCC AAACAGACAT 
CGCCACCGCT CTGCAGACCC CTACCGTTAT ACCGCCACTG AGAGAAACAA TGAAAAGCCA 
ACGGTAGATG AGGCTGCAAT GACCGGGACT CCAGAACCAA GCCTCCCTTT TGAAGAGAGT 
ATCCTTCGGG AGTCGCCGGC CCTGGACCGC CTGAACAGGG ACCCCACTCT TTCACTTCCA 
CCACTACAGA GAGAGGATCG TCTCTACGGG CAGGACAGTC TTCTAATGAG AACCTCCTCC 
ATGGAAAGAG CCTACAGAGG AGACAGACTT GGCATGGCAT CCCGTGACAA CTTCAAAGAC 
GTCACCCTGC CCAGAGTCCG CACCCCTGAT TTTGATTCAT CCTACCAGAA GTACACCTCC 
CTGTTGAGAG GGCGTACTCC TGAGATGGTT GAGAGGGATG GGCTCTTTGC CAGTCGAGGT 
AGCACCCCTG AACCCCGATA CCGTGCTCGC AGCCTTGGAC GGGCCATCTC CCCAGCCAGG 
AGGGACGACT CGGAGGATGA AGAAGATGAC GATCATTTTG TTGCGGCTAA GGTGAGAGAG 
TACTACAGCA CACTCAAGTC AGACACCAGC CGCCAAACCA AGCCTATGAT CCCTGAGCCT 
AAGAAGACCT ACAAGGATAA TCCCAAAGAC CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015240763.1
CDS	388..4689
Translation	MTNPALKKNHWTFRVTECSVGKDARGDLNVSLRGGAENGEFSYIGHINEELVFYREGRLNEGELLLEVENISISGLPLYDVQTLIKNCKSPVKLKTVRPGTKLNKDLKHYLSQRFQKSSPDHELQQTIRENLYRHAVPCTTRSPREGEVPGVDYNFLSVEDFLELEKSGTLLEIGTYDGNYYGTPKPPVQPPIGKVINTSGSGGDALLSDGLSGSLPGSQNSTPRRAKSYNDMQNAGIGLTDNQLEDDEDLPDMNSSFTGDSSEIDELHHSARPHVPRYSDPSYAGTTPSPSATTESTQQTNLHLSHPPPEDPLGPLPDNWEMAYTENGEVYFIDHNTKTTSWLDPRCLDKPQKPLEECEDDEGVHTEELDDLELPPGWEKIDDPVYGVYYVDHINRKTQYENPVLEAKRRRQLEQQPAQQPQPQSQQPPEGERYIREWIEDSALAGAPLATYTANHETYRDPQTGPPVPNAMGQKRVAPAKKSPKLQLSRKDSQNSSQHSVSSHRSAHTDSPVHSSLAPPLSESSTPPAPSQPLPGLPSQDSQGDGTTHRKPDPFKIWAQSRSMYESRLPDFQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTSYTVKSEGDVPPSPASSHHSSTQAPSLTEDMGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGGGGAGVTGNAVVSASTVATTSSQPPNGTTNAAPASAVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGNSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNASLLTNAEKIATITTTHTAQQQAAPEARTNTKPKQESYEFKAPTNTAPQPPIQQDSAQDSEFYSVDLERDNKGFGFSLRGGREYNMDLYVLRLAEEGAAVRNGKMRVGDEILEINGESTKGMKHARAIELIKNGGRNVHLVLKRGDGSVPEYDGSPNDISTVDPVTRIQNAAEVSSLPLTNAPSESSFPPEPPPHSRSKTEPSRRTHGTGKPHRSHHRHHSPSKSKGKKSKGRHGHKQNKKKDDKEKEGHRHRSRHRSPHRGQGRSRSADNMLDSRDRGHRRGRSPERRHGQHSSNHRHGSPRRSPYRRSPTRSPYRRSPYRSPRRSPYRSHRHSPRRSPHRHRSPNRYHSPNRHRHRSADPYRYTATERNNEKPTVDEAAMTGTPEPSLPFEESILRESPALDRLNRDPTLSLPPLQREDRLYGQDSLLMRTSSMERAYRGDRLGMASRDNFKDVTLPRVRTPDFDSSYQKYTSLLRGRTPEMVERDGLFASRGSTPEPRYRARSLGRAISPARRDDSEDEEDDDHFVAAKVREYYSTLKSDTSRQTKPMIPEPKKTYKDNPKDLSI

Target ORF information:

RefSeq Version	XM_015385277.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107091395), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385277.1

ORF Insert Sequence:

ATGACCAACC CTGCGCTGAA GAAGAACCAC TGGACCTTCA GGGTGACCGA ATGCTCCGTG 
GGGAAAGATG CCCGTGGAGA CCTGAACGTG TCTCTGCGCG GCGGCGCGGA GAACGGGGAG 
TTCTCCTACA TCGGCCACAT TAACGAGGAG CTGGTGTTTT ACAGAGAGGG CAGACTGAAC 
GAAGGGGAGC TGCTGCTGGA GGTGGAGAAC ATCTCCATCT CAGGATTACC TCTGTACGAC 
GTGCAGACGC TGATAAAGAA CTGCAAAAGT CCCGTGAAGT TGAAAACTGT GAGACCAGGA 
ACTAAGTTGA ATAAGGACCT GAAGCATTAT CTAAGCCAAC GCTTCCAGAA GTCCTCACCT 
GACCACGAAC TGCAGCAGAC CATCAGAGAA AACCTGTACC GCCACGCAGT GCCTTGTACT 
ACCCGCTCCC CGCGTGAAGG AGAGGTTCCT GGGGTGGATT ATAACTTCCT GTCTGTAGAG 
GACTTCCTCG AGTTGGAGAA GAGTGGAACC CTGCTGGAAA TTGGAACATA TGACGGTAAC 
TATTATGGGA CGCCCAAGCC GCCGGTGCAG CCCCCCATCG GGAAAGTGAT TAACACCAGC 
GGTAGCGGTG GTGATGCCCT GCTGTCAGAC GGGCTCAGCG GTAGCCTGCC GGGCTCGCAG 
AACTCCACTC CCCGACGAGC CAAGTCCTAC AATGACATGC AAAATGCTGG AATAGGGCTT 
ACAGATAACC AACTGGAGGA CGACGAGGAC CTCCCTGACA TGAACAGCAG CTTTACAGGA 
GATTCTAGCG AAATAGACGA GCTTCATCAC TCAGCGCGCC CCCACGTCCC TCGCTATAGT 
GACCCGTCCT ACGCTGGGAC AACCCCCTCA CCATCGGCCA CCACGGAGAG CACACAGCAG 
ACAAACCTTC ACCTGAGCCA TCCTCCCCCG GAGGACCCGC TGGGACCTCT GCCTGACAAC 
TGGGAGATGG CCTACACCGA GAATGGAGAG GTCTACTTTA TAGACCACAA TACAAAGACC 
ACCTCTTGGT TAGACCCTCG GTGCCTGGAC AAACCTCAGA AACCCCTGGA AGAATGTGAA 
GATGATGAAG GGGTCCATAC AGAAGAGCTG GATGATCTCG AACTTCCTCC AGGATGGGAG 
AAAATTGATG ATCCAGTCTA TGGGGTCTAC TATGTTGATC ATATCAACAG GAAGACCCAG 
TATGAAAACC CAGTGCTGGA GGCTAAGAGG CGCAGGCAGC TGGAACAGCA GCCCGCCCAG 
CAGCCTCAGC CCCAAAGCCA GCAGCCACCT GAAGGTGAGC GCTACATCCG AGAATGGATC 
GAGGACTCCG CATTGGCAGG CGCCCCACTG GCTACCTACA CTGCCAACCA CGAGACCTAC 
AGGGACCCTC AAACAGGTCC GCCTGTTCCC AACGCCATGG GACAAAAACG AGTGGCACCT 
GCTAAGAAGA GCCCCAAACT GCAATTGTCC AGGAAGGACA GTCAGAACAG TTCCCAGCAT 
AGCGTCTCCA GCCACCGGAG CGCCCACACC GACTCGCCTG TGCACTCGTC TCTGGCACCC 
CCTCTCAGTG AGAGCAGCAC GCCGCCGGCC CCGTCGCAGC CTTTGCCTGG CCTGCCTTCC 
CAGGACTCTC AAGGAGACGG GACCACCCAC CGAAAACCAG ACCCCTTTAA GATCTGGGCC 
CAGTCCAGGA GCATGTATGA AAGTAGGCTT CCAGACTTCC AGGAGCAGGA CATTTTCCTG 
TGGAGGAAGG ACACAGGCTT CGGCTTTAGG ATCCTTGGTG GCAACGAGCC AGGAGAACCT 
ATCTACATCG GCCACATAGT AAAGTACGGC GCTGCAGATG AGGACGGCCG CCTCCGATCC 
GGAGATGAAC TGATATGCGT GGACGGCACG GCGGTGGTGG GCAAGTCGCA TCAATTGGTG 
GTGCAGTTGA TGCAGCAGGC TGCCAAGCAG GGCCATGTTA ATCTTACTGT GCGACGCAAA 
ACCAGCTACA CAGTTAAATC AGAAGGAGAC GTCCCTCCGT CACCAGCGTC CTCACACCAC 
AGCAGCACCC AGGCGCCCAG CCTGACGGAG GACATGGGGA AGCGCACCCC GCAAGGCAGC 
CAGAATTCCC TCAACACGGT CAGCTCGGGC AGCGGCTCCA CCTCTGGCAT CGGCAGCGGT 
GGAGGAGGGG GAGGCGGGGG GGCCGGCGTC ACTGGGAATG CTGTGGTTTC CGCCTCGACG 
GTTGCGACCA CATCCTCTCA GCCTCCCAAC GGCACCACAA ACGCTGCCCC CGCCTCGGCC 
GTTCAGCCCT ACGACGTGGA GATCCGACGG GGCGAGAATG AAGGGTTCGG TTTCGTCATC 
GTGTCATCAG TGTCGCGGCC TGAAGCTGGC ACCACCTTCG CTGGAAATGC ATGTGTGGCC 
ATGCCTCACA AAATAGGCCG CATCATCGAG GGGAGTCCAG CGGACCGCTG CGGGAAGCTG 
AAGGTGGGCG ACCGCATCCT GGCTGTCAAC GGGAACTCCA TCACCAACAA GTCCCACTCT 
GACATCGTCA ACCTGATCAA GGAGGCTGGG AACACCGTCA CGCTGCGGAT CATTCCTGGA 
GATGAGTCAT CCAATGCATC TTTGTTGACA AATGCAGAGA AGATTGCCAC CATAACCACC 
ACTCACACAG CTCAGCAACA GGCAGCACCT GAGGCCAGGA CCAACACTAA ACCAAAACAG 
GAATCTTATG AGTTTAAAGC TCCTACGAAC ACTGCTCCCC AGCCCCCCAT CCAGCAAGAC 
TCAGCTCAGG ACTCCGAGTT CTACTCTGTG GACCTGGAGA GGGACAACAA AGGCTTTGGC 
TTCAGCCTGC GGGGAGGAAG AGAGTACAAC ATGGACCTGT ACGTGCTGAG GCTTGCTGAA 
GAAGGAGCTG CTGTTCGCAA TGGGAAAATG AGGGTTGGAG ATGAGATCTT GGAGATCAAC 
GGCGAAAGCA CCAAAGGCAT GAAGCACGCA CGGGCCATCG AGCTCATCAA GAACGGAGGC 
CGAAACGTCC ACCTGGTGCT CAAGAGGGGT GACGGCTCCG TGCCTGAATA TGACGGCAGC 
CCCAACGACA TCAGCACAGT TGACCCAGTC ACAAGGATAC AAAACGCTGC GGAAGTGAGT 
TCCCTGCCTC TAACTAATGC ACCATCAGAG TCCAGCTTCC CACCAGAACC CCCACCTCAT 
TCACGGAGCA AAACGGAGCC GAGCCGTCGC ACCCATGGCA CTGGCAAGCC CCATCGTTCC 
CATCATCGTC ATCACTCCCC TAGCAAATCC AAAGGGAAGA AGTCTAAAGG AAGGCATGGA 
CACAAGCAGA ATAAGAAAAA GGATGACAAG GAAAAAGAAG GCCATCGGCA TCGAAGCCGC 
CACCGCTCGC CTCATAGGGG GCAGGGCCGT TCACGTAGTG CAGACAACAT GCTGGATAGC 
CGTGATAGAG GGCACCGCCG AGGCCGTTCA CCAGAACGGC GCCACGGCCA ACACTCCTCG 
AACCACCGCC ACGGCTCTCC TCGACGCTCC CCTTATCGCC GCTCTCCAAC CCGCTCACCA 
TACCGACGCT CCCCATACCG CTCTCCTAGA CGCTCCCCTT ACCGCTCTCA TCGCCACTCT 
CCTCGACGTT CTCCTCATCG CCACAGGTCC CCCAACCGCT ACCACTCTCC AAACAGACAT 
CGCCACCGCT CTGCAGACCC CTACCGTTAT ACCGCCACTG AGAGAAACAA TGAAAAGCCA 
ACGGTAGATG AGGCTGCAAT GACCGGGACT CCAGAACCAA GCCTCCCTTT TGAAGAGAGT 
ATCCTTCGGG AGTCGCCGGC CCTGGACCGC CTGAACAGGG ACCCCACTCT TTCACTTCCA 
CCACTACAGA GAGAGGATCG TCTCTACGGG CAGGACAGTC TTCTAATGAG AACCTCCTCC 
ATGGAAAGAG CCTACAGAGG AGACAGACTT GGCATGGCAT CCCGTGACAA CTTCAAAGAC 
GTCACCCTGC CCAGAGTCCG CACCCCTGAT TTTGATTCAT CCTACCAGAA GTACACCTCC 
CTGTTGAGAG GGCGTACTCC TGAGATGGTT GAGAGGGATG GGCTCTTTGC CAGTCGAGGT 
AGCACCCCTG AACCCCGATA CCGTGCTCGC AGCCTTGGAC GGGCCATCTC CCCAGCCAGG 
AGGGACGACT CGGAGGATGA AGAAGATGAC GATCATTTTG TTGCGGCTAA GGTGAGAGAG 
TACTACAGCA CACTCAAGTC AGACACCAGC CGCCAAACCA AGCCTATGAT CCCTGAGCCT 
AAGAAGACCT ACAAGGATAA TCCCAAAGAC CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC107091395 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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