crim1 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	107084074
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015373736.1	XP_015229222.1	cysteine-rich motor neuron 1 protein isoform X1
XM_015373737.1	XP_015229223.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy06701	XM_015373736.1 Latest version!	Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCy06702	XM_015373737.1 Latest version!	Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_015373736.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015229222.1 Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy06701
Clone ID Related Accession (Same CDS sequence)	XM_015373736.1
Accession Version	XM_015373736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAA AACGCACCGT TATATTGGTG AATCTCCTCG TCCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACTTGTCCAC TCAAAGTCGC GCGCGGCTGT 
GAGGGTGGCC GCAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CGCGCGGCTG 
GAGTGGGAGC CCTGCGGCGG GAAGGACTGG GCGCACGGAT ACTGCGCCTT GGGGCTGACG 
TGCGCTACTG TCAACAGCAC TGGAGCCGCC ATCATACCTG AAATTGGAGT TTGTAAATTT 
CTCCCTGACC AGCCAGATGC AGGCCTTGAA GATGAGCGCT GCCCTCAAAT CACTGGCTGC 
GACAAAGCAG GAGATGAATG CGTGTGCAGT GCTCGTAACT CTTGCTTGGA AACCTTTACC 
TATCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG CCACGAGCAT 
AGGGAGAGAC GCAAAGAGAA ATATGTGGGA CCGTCCAATC CGACGTGCAT GTTCTCAGGG 
TGCAACCTGA CCGCCAACGG CTGCATGTGC AAGTCTCAGA GCTGCCACCA CCACTTTGCA 
TACATCAACC GCAGCCAGTG CGAGGAAGCA GCAGAGGAAC TGAGGTGCGC CGGGGTGATA 
TGTCCTGCTC TACATCTGCC CTCTTGCCCC ATAGGGTCTG TGCTGACCAA GAGCTACACT 
CCCCCTGCTG GCTGCTGCCC CTCAGTACCA CCCCAGTGCA CGTGCGACTT CCGATCCTGC 
AACAAAACCC ACTGCCCAAG TGGCCAGCAT GCTGTTCTGA TTAAACAGGC CAGCGGCCGG 
CCAGGAGACT GCTGTGACTT TTTTGAGTGC CAGAAAGTTT CTCCAAAATG CGTCTTCAGT 
GGGAAGGAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGTGGTGGAA TCTCCTTCTG CACCAAGGCT GAGTGTGCTG AGCTGGACTG TGAAAGGTTT 
TACATTCCAG AGGGAGAGTG CTGCCCCGTC TGCATAGATG TTGAGTTGCA GTCAATGGAC 
AGCACTAAGG CCAGTTGCTG GATGAATAAC AAGTTGCGAA TGCACGAGGA GCAGTGGAAA 
GAGGACGACT GTACATTCTG CCAGTGTGTG GATGGAGTAC CACATTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAAAA CCCTGTGAAG ATTCCCGGAG AGTGCTGTCC CTTTTGTGAA 
GAGCCATCTT ATGAAACTGT AAGTCCCTTG CTATGTCCAC CGCTGGAAAA CTGCTCCCTC 
TCAGGGTTTG AATGTCCATA TGGCTTCCAG CAAGACCAAA ATGGGTGTCT CCTCTGCCAG 
TGCCTTAACA ATAACTCCTG CCCCGACTTG GGAAAATACT GTTCTCTTCA ATGTCCTATG 
GGATATGAGA GGGACGATCT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC TACTCCCAAG 
TGCCGACCCC TGACCTGCAC AAAGACCTGC CCTTTTGGAT ACGTGAGGAA CAAGCATGGC 
TGTGAAATGT GTCGATGTGT CAAATGCCCC CCCTTTACCT GCGACAAGCA CTGCAGCAAT 
GGCTATCAAC AAAACAGGAA GGGCTGCAAC ATATGCATGT GTAAAGAAAG TGATCAAGTC 
CTAAGCACGA CTGTCCCAGC CCCTACATCC AGTTTCTGCC TCACCTCCAA TGGGCGGCAG 
TATGAGGAAG GAGATAGCTG GCATGACGGC TGCCGTGACT GCTACTGCCA CTCTGGACGG 
GAGATGTGCG TGCTCATTTC ATGCCCCATG CCCAGCTGCT CTCATCCTGT TGTCAAGTCT 
GACCAGTGTT GTCCTACATG TGAAGACGAG TCTGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA CTTTATGTGG AGGGGGAGAC TTGGAACCTG 
GATGAATGCA CGCGTTGCAC CTGCAGGAAG GGACGCGTCC TGTGTGACAC TGAAGGTTGT 
CCACCAGCTC TCTGCCAAAC CCCCATCAGA AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GCAGAAGAGA CAGACATCAA CTTGCTGCCA GTGAACCGCA ACCAGCAGCA GTTCTGCATA 
ACCATTGAAG GAGACATTCT TCAAGCTGGG GACTCGTGGA AGGCCAATGC TTGTACAAGC 
TGCACCTGCA TCAACGGCAC CATTCAGTGT TTCTCTCAGC GCTGTCCAGC AGCCAGCTGT 
AGGGTGCCAG TTTTACGCAA GGGCCAATGC TGCCCATATT GTCTCGAATC AACCACATCA 
GCACCTGTCA CTCTGTCAAC CAGCAAACCA AAGGCAGGGG TCACCACCAC AGGGCAGAGA 
GGACGTTGGA TTACGACTGT GACCATCCCA CTTGTCATCA CTGATACAGA CCAGCCGCGA 
TTGGCTGAAA ACAACCCGGG CCATATGGAG ATGGCCCTTG TCTACCAATC AGCAGCATGG 
ATCCTTGCAG GGCTGCTTTT GGCCATAGTC ATCTTTCTCA TTGTGGCTTT GATCATCAAC 
AAGAAAAAGA AATGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC CGTGATCCTG 
AAGAAACATG TGAACAAGAA TTCTGTCATC TACATGGAGC CCTCAAAAGA GAACAAGTTT 
CAGAACAAGA GCAACTGTGG CATCATCCAC TTTTCCCCTG AGATGAGCTC GCCTCCCTGC 
ATGGAGAAGA TGAACATCCC CAGGGCCAAG CTGAGCACGG GCAGTGACTG GATGCCACGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015229222.1
CDS	303..3125
Translation	MFEKRTVILVNLLVLVRALLAHECTPCNLRTCPLKVARGCEGGRSVARDPCGCCDHCARLEWEPCGGKDWAHGYCALGLTCATVNSTGAAIIPEIGVCKFLPDQPDAGLEDERCPQITGCDKAGDECVCSARNSCLETFTYPDQETCMKASKSEGRSHEHRERRKEKYVGPSNPTCMFSGCNLTANGCMCKSQSCHHHFAYINRSQCEEAAEELRCAGVICPALHLPSCPIGSVLTKSYTPPAGCCPSVPPQCTCDFRSCNKTHCPSGQHAVLIKQASGRPGDCCDFFECQKVSPKCVFSGKEFSEGEVYRMDPCWLCQCRGGISFCTKAECAELDCERFYIPEGECCPVCIDVELQSMDSTKASCWMNNKLRMHEEQWKEDDCTFCQCVDGVPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGFECPYGFQQDQNGCLLCQCLNNNSCPDLGKYCSLQCPMGYERDDLGCEVCECSIPTPKCRPLTCTKTCPFGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYQQNRKGCNICMCKESDQVLSTTVPAPTSSFCLTSNGRQYEEGDSWHDGCRDCYCHSGREMCVLISCPMPSCSHPVVKSDQCCPTCEDESGSGQPEGMDMVVCRAPGGELYVEGETWNLDECTRCTCRKGRVLCDTEGCPPALCQTPIRNKDTCCHVCPAEETDINLLPVNRNQQQFCITIEGDILQAGDSWKANACTSCTCINGTIQCFSQRCPAASCRVPVLRKGQCCPYCLESTTSAPVTLSTSKPKAGVTTTGQRGRWITTVTIPLVITDTDQPRLAENNPGHMEMALVYQSAAWILAGLLLAIVIFLIVALIINKKKKWVQMSCYSAPKKTVILKKHVNKNSVIYMEPSKENKFQNKSNCGIIHFSPEMSSPPCMEKMNIPRAKLSTGSDWMPR

Target ORF information:

RefSeq Version	XM_015373736.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015373736.1

ORF Insert Sequence:

ATGTTTGAAA AACGCACCGT TATATTGGTG AATCTCCTCG TCCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACTTGTCCAC TCAAAGTCGC GCGCGGCTGT 
GAGGGTGGCC GCAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CGCGCGGCTG 
GAGTGGGAGC CCTGCGGCGG GAAGGACTGG GCGCACGGAT ACTGCGCCTT GGGGCTGACG 
TGCGCTACTG TCAACAGCAC TGGAGCCGCC ATCATACCTG AAATTGGAGT TTGTAAATTT 
CTCCCTGACC AGCCAGATGC AGGCCTTGAA GATGAGCGCT GCCCTCAAAT CACTGGCTGC 
GACAAAGCAG GAGATGAATG CGTGTGCAGT GCTCGTAACT CTTGCTTGGA AACCTTTACC 
TATCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG CCACGAGCAT 
AGGGAGAGAC GCAAAGAGAA ATATGTGGGA CCGTCCAATC CGACGTGCAT GTTCTCAGGG 
TGCAACCTGA CCGCCAACGG CTGCATGTGC AAGTCTCAGA GCTGCCACCA CCACTTTGCA 
TACATCAACC GCAGCCAGTG CGAGGAAGCA GCAGAGGAAC TGAGGTGCGC CGGGGTGATA 
TGTCCTGCTC TACATCTGCC CTCTTGCCCC ATAGGGTCTG TGCTGACCAA GAGCTACACT 
CCCCCTGCTG GCTGCTGCCC CTCAGTACCA CCCCAGTGCA CGTGCGACTT CCGATCCTGC 
AACAAAACCC ACTGCCCAAG TGGCCAGCAT GCTGTTCTGA TTAAACAGGC CAGCGGCCGG 
CCAGGAGACT GCTGTGACTT TTTTGAGTGC CAGAAAGTTT CTCCAAAATG CGTCTTCAGT 
GGGAAGGAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGTGGTGGAA TCTCCTTCTG CACCAAGGCT GAGTGTGCTG AGCTGGACTG TGAAAGGTTT 
TACATTCCAG AGGGAGAGTG CTGCCCCGTC TGCATAGATG TTGAGTTGCA GTCAATGGAC 
AGCACTAAGG CCAGTTGCTG GATGAATAAC AAGTTGCGAA TGCACGAGGA GCAGTGGAAA 
GAGGACGACT GTACATTCTG CCAGTGTGTG GATGGAGTAC CACATTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAAAA CCCTGTGAAG ATTCCCGGAG AGTGCTGTCC CTTTTGTGAA 
GAGCCATCTT ATGAAACTGT AAGTCCCTTG CTATGTCCAC CGCTGGAAAA CTGCTCCCTC 
TCAGGGTTTG AATGTCCATA TGGCTTCCAG CAAGACCAAA ATGGGTGTCT CCTCTGCCAG 
TGCCTTAACA ATAACTCCTG CCCCGACTTG GGAAAATACT GTTCTCTTCA ATGTCCTATG 
GGATATGAGA GGGACGATCT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC TACTCCCAAG 
TGCCGACCCC TGACCTGCAC AAAGACCTGC CCTTTTGGAT ACGTGAGGAA CAAGCATGGC 
TGTGAAATGT GTCGATGTGT CAAATGCCCC CCCTTTACCT GCGACAAGCA CTGCAGCAAT 
GGCTATCAAC AAAACAGGAA GGGCTGCAAC ATATGCATGT GTAAAGAAAG TGATCAAGTC 
CTAAGCACGA CTGTCCCAGC CCCTACATCC AGTTTCTGCC TCACCTCCAA TGGGCGGCAG 
TATGAGGAAG GAGATAGCTG GCATGACGGC TGCCGTGACT GCTACTGCCA CTCTGGACGG 
GAGATGTGCG TGCTCATTTC ATGCCCCATG CCCAGCTGCT CTCATCCTGT TGTCAAGTCT 
GACCAGTGTT GTCCTACATG TGAAGACGAG TCTGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA CTTTATGTGG AGGGGGAGAC TTGGAACCTG 
GATGAATGCA CGCGTTGCAC CTGCAGGAAG GGACGCGTCC TGTGTGACAC TGAAGGTTGT 
CCACCAGCTC TCTGCCAAAC CCCCATCAGA AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GCAGAAGAGA CAGACATCAA CTTGCTGCCA GTGAACCGCA ACCAGCAGCA GTTCTGCATA 
ACCATTGAAG GAGACATTCT TCAAGCTGGG GACTCGTGGA AGGCCAATGC TTGTACAAGC 
TGCACCTGCA TCAACGGCAC CATTCAGTGT TTCTCTCAGC GCTGTCCAGC AGCCAGCTGT 
AGGGTGCCAG TTTTACGCAA GGGCCAATGC TGCCCATATT GTCTCGAATC AACCACATCA 
GCACCTGTCA CTCTGTCAAC CAGCAAACCA AAGGCAGGGG TCACCACCAC AGGGCAGAGA 
GGACGTTGGA TTACGACTGT GACCATCCCA CTTGTCATCA CTGATACAGA CCAGCCGCGA 
TTGGCTGAAA ACAACCCGGG CCATATGGAG ATGGCCCTTG TCTACCAATC AGCAGCATGG 
ATCCTTGCAG GGCTGCTTTT GGCCATAGTC ATCTTTCTCA TTGTGGCTTT GATCATCAAC 
AAGAAAAAGA AATGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC CGTGATCCTG 
AAGAAACATG TGAACAAGAA TTCTGTCATC TACATGGAGC CCTCAAAAGA GAACAAGTTT 
CAGAACAAGA GCAACTGTGG CATCATCCAC TTTTCCCCTG AGATGAGCTC GCCTCCCTGC 
ATGGAGAAGA TGAACATCCC CAGGGCCAAG CTGAGCACGG GCAGTGACTG GATGCCACGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_015373737.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015229223.1 Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy06702
Clone ID Related Accession (Same CDS sequence)	XM_015373737.1
Accession Version	XM_015373737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAA AACGCACCGT TATATTGGTG AATCTCCTCG TCCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACTTGTCCAC TCAAAGTCGC GCGCGGCTGT 
GAGGGTGGCC GCAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CGCGCGGCTG 
GAGTGGGAGC CCTGCGGCGG GAAGGACTGG GCGCACGGAT ACTGCGCCTT GGGGCTGACG 
TGCGCTACTG TCAACAGCAC TGGAGCCGCC ATCATACCTG AAATTGGAGT TTGTAAATTT 
CTCCCTGACC AGCCAGATGC AGGCCTTGAA GATGAGCGCT GCCCTCAAAT CACTGGCTGC 
GACAAAGCAG GAGATGAATG CGTGTGCAGT GCTCGTAACT CTTGCTTGGA AACCTTTACC 
TATCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG CCACGAGCAT 
AGGGAGAGAC GCAAAGAGAA ATATGTGGGA CCGTCCAATC CGACGTGCAT GTTCTCAGGG 
TGCAACCTGA CCGCCAACGG CTGCATGTGC AAGTCTCAGA GCTGCCACCA CCACTTTGCA 
TACATCAACC GCAGCCAGTG CGAGGAAGCA GCAGAGGAAC TGAGGTGCGC CGGGGTGATA 
TGTCCTGCTC TACATCTGCC CTCTTGCCCC ATAGGGTCTG TGCTGACCAA GAGCTACACT 
CCCCCTGCTG GCTGCTGCCC CTCAGTACCA CCCCAGTGCA CGTGCGACTT CCGATCCTGC 
AACAAAACCC ACTGCCCAAG TGGCCAGCAT GCTGTTCTGA TTAAACAGGC CAGCGGCCGG 
CCAGGAGACT GCTGTGACTT TTTTGAGTGC CAGAAAGTTT CTCCAAAATG CGTCTTCAGT 
GGGAAGGAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGTGGTGGAA TCTCCTTCTG CACCAAGGCT GAGTGTGCTG AGCTGGACTG TGAAAGGTTT 
TACATTCCAG AGGGAGAGTG CTGCCCCGTC TGCATAGATG TTGAGTTGCA GTCAATGGAC 
AGCACTAAGG CCAGTTGCTG GATGAATAAC AAGTTGCGAA TGCACGAGGA GCAGTGGAAA 
GAGGACGACT GTACATTCTG CCAGTGTGTG GATGGAGTAC CACATTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAAAA CCCTGTGAAG ATTCCCGGAG AGTGCTGTCC CTTTTGTGAA 
GAGCCATCTT ATGAAACTGT AAGTCCCTTG CTATGTCCAC CGCTGGAAAA CTGCTCCCTC 
TCAGGGTTTG AATGTCCATA TGGCTTCCAG CAAGACCAAA ATGGGTGTCT CCTCTGCCAG 
TGCCTTAACA ATAACTCCTG CCCCGACTTG GGAAAATACT GTTCTCTTCA ATGTCCTATG 
GGATATGAGA GGGACGATCT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC TACTCCCAAG 
TGCCGACCCC TGACCTGCAC AAAGACCTGC CCTTTTGGAT ACGTGAGGAA CAAGCATGGC 
TGTGAAATGT GTCGATGTGT CAAATGCCCC CCCTTTACCT GCGACAAGCA CTGCAGCAAT 
GGCTATCAAC AAAACAGGAA GGGCTGCAAC ATATGCATGT GTAAAGAAAG TGATCAAGTC 
CTAAGCACGA CTGTCCCAGC CCCTACATCC AGTTTCTGCC TCACCTCCAA TGGGCGGCAG 
TATGAGGAAG GAGATAGCTG GCATGACGGC TGCCGTGACT GCTACTGCCA CTCTGGACGG 
GAGATGTGCG TGCTCATTTC ATGCCCCATG CCCAGCTGCT CTCATCCTGT TGTCAAGTCT 
GACCAGTGTT GTCCTACATG TGAAGACGAG TCTGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA CTTTATGTGG AGGGGGAGAC TTGGAACCTG 
GATGAATGCA CGCGTTGCAC CTGCAGGAAG GGACGCGTCC TGTGTGACAC TGAAGGTTGT 
CCACCAGCTC TCTGCCAAAC CCCCATCAGA AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GAAGAGACAG ACATCAACTT GCTGCCAGTG AACCGCAACC AGCAGCAGTT CTGCATAACC 
ATTGAAGGAG ACATTCTTCA AGCTGGGGAC TCGTGGAAGG CCAATGCTTG TACAAGCTGC 
ACCTGCATCA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCAGCAGC CAGCTGTAGG 
GTGCCAGTTT TACGCAAGGG CCAATGCTGC CCATATTGTC TCGAATCAAC CACATCAGCA 
CCTGTCACTC TGTCAACCAG CAAACCAAAG GCAGGGGTCA CCACCACAGG GCAGAGAGGA 
CGTTGGATTA CGACTGTGAC CATCCCACTT GTCATCACTG ATACAGACCA GCCGCGATTG 
GCTGAAAACA ACCCGGGCCA TATGGAGATG GCCCTTGTCT ACCAATCAGC AGCATGGATC 
CTTGCAGGGC TGCTTTTGGC CATAGTCATC TTTCTCATTG TGGCTTTGAT CATCAACAAG 
AAAAAGAAAT GGGTGCAGAT GTCCTGCTAC AGCGCACCAA AGAAGACCGT GATCCTGAAG 
AAACATGTGA ACAAGAATTC TGTCATCTAC ATGGAGCCCT CAAAAGAGAA CAAGTTTCAG 
AACAAGAGCA ACTGTGGCAT CATCCACTTT TCCCCTGAGA TGAGCTCGCC TCCCTGCATG 
GAGAAGATGA ACATCCCCAG GGCCAAGCTG AGCACGGGCA GTGACTGGAT GCCACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015229223.1
CDS	303..3122
Translation	MFEKRTVILVNLLVLVRALLAHECTPCNLRTCPLKVARGCEGGRSVARDPCGCCDHCARLEWEPCGGKDWAHGYCALGLTCATVNSTGAAIIPEIGVCKFLPDQPDAGLEDERCPQITGCDKAGDECVCSARNSCLETFTYPDQETCMKASKSEGRSHEHRERRKEKYVGPSNPTCMFSGCNLTANGCMCKSQSCHHHFAYINRSQCEEAAEELRCAGVICPALHLPSCPIGSVLTKSYTPPAGCCPSVPPQCTCDFRSCNKTHCPSGQHAVLIKQASGRPGDCCDFFECQKVSPKCVFSGKEFSEGEVYRMDPCWLCQCRGGISFCTKAECAELDCERFYIPEGECCPVCIDVELQSMDSTKASCWMNNKLRMHEEQWKEDDCTFCQCVDGVPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSGFECPYGFQQDQNGCLLCQCLNNNSCPDLGKYCSLQCPMGYERDDLGCEVCECSIPTPKCRPLTCTKTCPFGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYQQNRKGCNICMCKESDQVLSTTVPAPTSSFCLTSNGRQYEEGDSWHDGCRDCYCHSGREMCVLISCPMPSCSHPVVKSDQCCPTCEDESGSGQPEGMDMVVCRAPGGELYVEGETWNLDECTRCTCRKGRVLCDTEGCPPALCQTPIRNKDTCCHVCPEETDINLLPVNRNQQQFCITIEGDILQAGDSWKANACTSCTCINGTIQCFSQRCPAASCRVPVLRKGQCCPYCLESTTSAPVTLSTSKPKAGVTTTGQRGRWITTVTIPLVITDTDQPRLAENNPGHMEMALVYQSAAWILAGLLLAIVIFLIVALIINKKKKWVQMSCYSAPKKTVILKKHVNKNSVIYMEPSKENKFQNKSNCGIIHFSPEMSSPPCMEKMNIPRAKLSTGSDWMPR

Target ORF information:

RefSeq Version	XM_015373737.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015373737.1

ORF Insert Sequence:

ATGTTTGAAA AACGCACCGT TATATTGGTG AATCTCCTCG TCCTGGTGCG TGCGCTCCTG 
GCTCACGAGT GCACGCCCTG TAACCTCCGG ACTTGTCCAC TCAAAGTCGC GCGCGGCTGT 
GAGGGTGGCC GCAGCGTGGC CAGGGATCCA TGCGGGTGCT GCGACCACTG CGCGCGGCTG 
GAGTGGGAGC CCTGCGGCGG GAAGGACTGG GCGCACGGAT ACTGCGCCTT GGGGCTGACG 
TGCGCTACTG TCAACAGCAC TGGAGCCGCC ATCATACCTG AAATTGGAGT TTGTAAATTT 
CTCCCTGACC AGCCAGATGC AGGCCTTGAA GATGAGCGCT GCCCTCAAAT CACTGGCTGC 
GACAAAGCAG GAGATGAATG CGTGTGCAGT GCTCGTAACT CTTGCTTGGA AACCTTTACC 
TATCCAGACC AAGAGACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG CCACGAGCAT 
AGGGAGAGAC GCAAAGAGAA ATATGTGGGA CCGTCCAATC CGACGTGCAT GTTCTCAGGG 
TGCAACCTGA CCGCCAACGG CTGCATGTGC AAGTCTCAGA GCTGCCACCA CCACTTTGCA 
TACATCAACC GCAGCCAGTG CGAGGAAGCA GCAGAGGAAC TGAGGTGCGC CGGGGTGATA 
TGTCCTGCTC TACATCTGCC CTCTTGCCCC ATAGGGTCTG TGCTGACCAA GAGCTACACT 
CCCCCTGCTG GCTGCTGCCC CTCAGTACCA CCCCAGTGCA CGTGCGACTT CCGATCCTGC 
AACAAAACCC ACTGCCCAAG TGGCCAGCAT GCTGTTCTGA TTAAACAGGC CAGCGGCCGG 
CCAGGAGACT GCTGTGACTT TTTTGAGTGC CAGAAAGTTT CTCCAAAATG CGTCTTCAGT 
GGGAAGGAGT TCTCAGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGTGGTGGAA TCTCCTTCTG CACCAAGGCT GAGTGTGCTG AGCTGGACTG TGAAAGGTTT 
TACATTCCAG AGGGAGAGTG CTGCCCCGTC TGCATAGATG TTGAGTTGCA GTCAATGGAC 
AGCACTAAGG CCAGTTGCTG GATGAATAAC AAGTTGCGAA TGCACGAGGA GCAGTGGAAA 
GAGGACGACT GTACATTCTG CCAGTGTGTG GATGGAGTAC CACATTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAAAA CCCTGTGAAG ATTCCCGGAG AGTGCTGTCC CTTTTGTGAA 
GAGCCATCTT ATGAAACTGT AAGTCCCTTG CTATGTCCAC CGCTGGAAAA CTGCTCCCTC 
TCAGGGTTTG AATGTCCATA TGGCTTCCAG CAAGACCAAA ATGGGTGTCT CCTCTGCCAG 
TGCCTTAACA ATAACTCCTG CCCCGACTTG GGAAAATACT GTTCTCTTCA ATGTCCTATG 
GGATATGAGA GGGACGATCT GGGCTGCGAG GTGTGTGAGT GCAGCATCCC TACTCCCAAG 
TGCCGACCCC TGACCTGCAC AAAGACCTGC CCTTTTGGAT ACGTGAGGAA CAAGCATGGC 
TGTGAAATGT GTCGATGTGT CAAATGCCCC CCCTTTACCT GCGACAAGCA CTGCAGCAAT 
GGCTATCAAC AAAACAGGAA GGGCTGCAAC ATATGCATGT GTAAAGAAAG TGATCAAGTC 
CTAAGCACGA CTGTCCCAGC CCCTACATCC AGTTTCTGCC TCACCTCCAA TGGGCGGCAG 
TATGAGGAAG GAGATAGCTG GCATGACGGC TGCCGTGACT GCTACTGCCA CTCTGGACGG 
GAGATGTGCG TGCTCATTTC ATGCCCCATG CCCAGCTGCT CTCATCCTGT TGTCAAGTCT 
GACCAGTGTT GTCCTACATG TGAAGACGAG TCTGGCAGTG GCCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA CTTTATGTGG AGGGGGAGAC TTGGAACCTG 
GATGAATGCA CGCGTTGCAC CTGCAGGAAG GGACGCGTCC TGTGTGACAC TGAAGGTTGT 
CCACCAGCTC TCTGCCAAAC CCCCATCAGA AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GAAGAGACAG ACATCAACTT GCTGCCAGTG AACCGCAACC AGCAGCAGTT CTGCATAACC 
ATTGAAGGAG ACATTCTTCA AGCTGGGGAC TCGTGGAAGG CCAATGCTTG TACAAGCTGC 
ACCTGCATCA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCAGCAGC CAGCTGTAGG 
GTGCCAGTTT TACGCAAGGG CCAATGCTGC CCATATTGTC TCGAATCAAC CACATCAGCA 
CCTGTCACTC TGTCAACCAG CAAACCAAAG GCAGGGGTCA CCACCACAGG GCAGAGAGGA 
CGTTGGATTA CGACTGTGAC CATCCCACTT GTCATCACTG ATACAGACCA GCCGCGATTG 
GCTGAAAACA ACCCGGGCCA TATGGAGATG GCCCTTGTCT ACCAATCAGC AGCATGGATC 
CTTGCAGGGC TGCTTTTGGC CATAGTCATC TTTCTCATTG TGGCTTTGAT CATCAACAAG 
AAAAAGAAAT GGGTGCAGAT GTCCTGCTAC AGCGCACCAA AGAAGACCGT GATCCTGAAG 
AAACATGTGA ACAAGAATTC TGTCATCTAC ATGGAGCCCT CAAAAGAGAA CAAGTTTCAG 
AACAAGAGCA ACTGTGGCAT CATCCACTTT TCCCCTGAGA TGAGCTCGCC TCCCTGCATG 
GAGAAGATGA ACATCCCCAG GGCCAAGCTG AGCACGGGCA GTGACTGGAT GCCACGCTAA