isg15 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	isg15
Entrez Gene ID	103397325
Full Name	ISG15 ubiquitin-like modifier
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301006.1	NP_001287935.1	ubiquitin-like protein ISG15

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cynoglossus semilaevis ISG15: a secreted cytokine-like protein that stimulates antiviral immune response in a LRGG motif-dependent manner.
Wang W, Zhang M, Xiao ZZ, Sun L
PloS one7(9)e44884(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following isg15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the isg15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy00019	NM_001301006.1 Latest version!	Cynoglossus semilaevis ISG15 ubiquitin-like modifier (isg15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

isg15 ( NM_001301006.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> NP_001287935.1 Cynoglossus semilaevis ISG15 ubiquitin-like modifier (isg15), mRNA.

Price & Availability↑

CloneID	OCy00019
Clone ID Related Accession (Same CDS sequence)	NM_001301006.1
Accession Version	NM_001301006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Cynoglossus semilaevis(tongue sole)
Product	ubiquitin-like protein ISG15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN967757.1. On Jul 17, 2014 this sequence version replaced XM_008335544.1. ##Evidence-Data-START## Transcript exon combination :: JN967757.1, GH231767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00752404, SAMN01766803 [ECO:0000348] ##Evidence-Data-END##

ATGGAAATAA CCATCACAAT GTTAAACGGG AACTCATGCA CTCTGATGGT GCAGCCTCAG 
GAGAACGTGG GCTCTCTGAG GCAGCGCATC CATCAGAAAC TGAATGTAAC CCCCGAGAGA 
CAGAGACTGG TGTTTGACAA TGGGCAGAGG AAGGATTTGA GCGACAACTC CCAGAACATA 
GCATTCTACG GTCTGCGTTC TGGCTCCAAA GTGTCCTTGC TGGTGATCGA ACCGGTCACC 
ATTCAGGTAT TTCTGAAGAA CGTCAAAAAC GTGGTCAGCG CCTACGACAT CACACCTGAC 
GAGACAGTGG CCAACTTTAA GCGCAGGGTG CAGCACAGAG AAGGCGTGGC AGAGAGTCAG 
CAGAGGCTGG TGTTCCAAGG CCAGGAGATG ACGCAAGGAA AACTGTCTGA TTACAACGTC 
CAAGCTCTGA GCACCATTGA GCTGTTACTG CGTCTGAGAG GAGGCAGAGG ACACACCGTC 
ATTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287935.1
CDS	58..546
Translation	MEITITMLNGNSCTLMVQPQENVGSLRQRIHQKLNVTPERQRLVFDNGQRKDLSDNSQNIAFYGLRSGSKVSLLVIEPVTIQVFLKNVKNVVSAYDITPDETVANFKRRVQHREGVAESQQRLVFQGQEMTQGKLSDYNVQALSTIELLLRLRGGRGHTVIG

Target ORF information:

RefSeq Version	NM_001301006.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ISG15 ubiquitin-like modifier (isg15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301006.1

ORF Insert Sequence:

ATGGAAATAA CCATCACAAT GTTAAACGGG AACTCATGCA CTCTGATGGT GCAGCCTCAG 
GAGAACGTGG GCTCTCTGAG GCAGCGCATC CATCAGAAAC TGAATGTAAC CCCCGAGAGA 
CAGAGACTGG TGTTTGACAA TGGGCAGAGG AAGGATTTGA GCGACAACTC CCAGAACATA 
GCATTCTACG GTCTGCGTTC TGGCTCCAAA GTGTCCTTGC TGGTGATCGA ACCGGTCACC 
ATTCAGGTAT TTCTGAAGAA CGTCAAAAAC GTGGTCAGCG CCTACGACAT CACACCTGAC 
GAGACAGTGG CCAACTTTAA GCGCAGGGTG CAGCACAGAG AAGGCGTGGC AGAGAGTCAG 
CAGAGGCTGG TGTTCCAAGG CCAGGAGATG ACGCAAGGAA AACTGTCTGA TTACAACGTC 
CAAGCTCTGA GCACCATTGA GCTGTTACTG CGTCTGAGAG GAGGCAGAGG ACACACCGTC 
ATTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cynoglossus semilaevis ISG15: a secreted cytokine-like protein that stimulates antiviral immune response in a LRGG motif-dependent manner.
PloS one7(9)e44884(2012)
Wang W,Zhang M,Xiao ZZ,Sun L