arap1 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	arap1
Entrez Gene ID	103378556
Full Name	ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025054874.1	XP_024910642.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_025054873.1	XP_024910641.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
XM_025054875.1	XP_024910643.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X2
XM_025054878.1	XP_024910646.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X4
XM_017032112.1	XP_016887601.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
XM_025054876.1	XP_024910644.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X3
XM_025054877.1	XP_024910645.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following arap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy200124	XM_025054878.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCy200121	XM_025054873.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCy200121	XM_025054874.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCy200122	XM_025054875.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCy200123	XM_025054877.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCy200123	XM_025054876.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCy53813	XM_017032112.1 Latest version!	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arap1 ( XM_025054878.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910646.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCy200124
Clone ID Related Accession (Same CDS sequence)	XM_025054878.1
Accession Version	XM_025054878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGG ACCAAGCTCC ACAGGTTCCA CCTCGAATCA GGACACCCCA ATGTCCAGAA 
AGCCTGCAGC AGTCGTCTCA ACTGGACAGT CAGCTGTCTC TGCCTGTGAG AAGAGTCTCC 
CAAACTCCCA GTGAGATCAC TCTAGATTTA CCTGGAAATA ATTCAGATGC AAATGAAGAG 
ACGGACATCC TTCACCTTAA TTCATTAGAC AAGGAAATGC TTGTGCAGGT GCAGAGTCCG 
ACAAAGGGAC ATTACAATTC GTGGTTAAGC GACGACGAGC TGTTGGATGA TGATGATGAT 
GATGATGATG ACGACGATGA TTATGATGAC AATGCTGCTG TGAGTCAGTT CCCGTGGCTT 
GACCGGCCCA TCAGTAACAT TCATGGCAGT GTGTGTCTGC CCAGCACCGG CAGACGGCCT 
TCGGCCGCTA AAAATGACAG CCCCGAGTTG ATTGCCACTG TCATCAAGAC GGGCTGGCTG 
GACAAGAATC CACCACAGGG GGTGCTTTAT TACCAGAGAC GATGGGTGAA ATTGGATGCG 
GATTATCTGC GATACTTTGA CAGTGAAAAG GACATTTATT CCAAGGGAAT CATCTCAACA 
GCCTTCATCA CTAACGTGGC GAGCATAGGA GAGCTAAAGT TTGAGGTGGC TACGAACAAC 
CGGACATTTA TCTTCAGGGC AGAAAATGAA GCTGAGAGAA ACGACTGGGT TGCCGTGCTC 
CTGGACTGCA CCAGAGGGCG CTACCGCCGC AGCAACATCG GCCCCGGTTC ACCCTTGGCC 
CCAGATCATC AGGGGTACCT GGAGCTCAAA GGGTTACGCT CCAAACTTTA CACTGTTGTT 
GCCCTCGATA AAGTCTTTCT CTACAAAACC ATCGATGACT ATCGTATGGG AGTCGGCATC 
ACCTCCATTG ATATGAATGT GGGAAATGTA AAAGATTCGG ACCGTCGTAA CTTCGAACTC 
ACAACGCCTT ACCGCATATT CAGTTTCATA GCGGAGTCGG AGCAGCAGAG GGAACTCTGG 
GTGGCCGCCA TGAGGAACGC TATAGGCGAG GCGCTGTCCA ATAGCGATGT GGCGAACCAG 
ATATGGGCGG AGCCTAGTAA CAGTTTCTGC GCCGACTGTG GGATTGCCAA ACCAGAATGG 
GCAGCTATCA ACTTGTGCGT GGTGGTCTGC AAGCAATGTG CAGGAGAGCA CAGAGGACTG 
GGTCCCAGCG TGTCCAAGGT GCGTAGTCTG AAGATGGACA GGAAGGTTTG GACAGAGGAA 
CTCGTACAGG TGTTTCTCTC GCTAGGCAAC GAGCGGGCCA ACAGCTTTTG GGCTGCTAAC 
GTCCCGCCCA GTGAGGCGCT GGCGCCTTCT AGCAGCAGCG AGGAGAGGAG GCGATTTATC 
ACCAACAAAT ATCGCCAGGG AAAGTACAGA AAGTACCACA TGCTGTATGG GAACCAGACT 
GAACTGAACA ATGCTTTATG TGTGAATATC CAATGCAGTG ATTTGTTGGA GACGTTGAGT 
TTGATCTTCT GCGGGGCAGA CGTAAATTGC TCGACTGGCA TGACAAACTG GCCGTCCCCT 
CTGTCCCTGG CCAACGCACG CTCACAGCAC TTACAGGCTG AAATAATCAG CCACAATCTC 
AACACAGATC AGCCTCAGTC AGAGGGGGTT GTGGCCATGC AGCCAGTTCC TTATTCAAAA 
CCACCTTGTG TGTCTTACAA TGGCTTTCTG TTCAAAACCG CATCCATGGC TCGAGCGGTC 
ACTGAGCGCA AGGCCAGAGA AGAGTTCAGC CGCCGTTGGT GCACGCTGAA CGACGGCGTC 
TTCAGCTATT ATGAGAGCGA CAAGAACTCC AACCCCAATG GAGCACTGAA GGTTTCAGAA 
ATAGTCAGTT TGGCTGTTGA CGCGCCTGAA AAACATGGAT ACGAATACAC CTTTGAAATC 
TACTCGGAGT CAGAGCGACT ATATCTGTTC GGCACCGATG ATGCCGACAG CCACAAAGAA 
TGGGTTAAGT CCATTGCTAG GAGTTTCATA CCCACTGCCG CAGAGCCGTT ACTGAAGATG 
AGTTTTGAGA GGATCGGACG GCTGAAATGT AAAGACGGCT TGAACCTGCA GACGTCTCGG 
CTGGGTTGGT TCGCTCTGGT GGGCTCCACA CTCCATGCCT CACTAGAGGA TGGGCAGTGT 
GAGGAAATCC ACCTCCGTAA AGTTAACGAA CTCTCCACAC AACAGGACAA TGGAGTGCTG 
GTTCTGGTGG AGAAAGGCAG GACTTTGTAC ATAGAAGGTG AGAGAAAGTT GGATTTCGCA 
GGCTGGTGCG CCGCCATCCG ATCGGCAGGA AGGAGCGGAG GAGACACGCT GAGTCAGCAG 
CAGCTCACCG AAACGGACAT ACCTGTCATC GTCCACAGCT GCATTGGATA CATCACTCAG 
TGCGGCTTGG CGTCTGAGGG CATTTATCGC AAAAGCGGCG TGAACTCGCG TGTCAAAGCT 
CTGTGCGAGA GATTTCGGGG TGATGCCCGC AGTCTTTGTC TAAGGGAGGG GGAGCACCAG 
GTGGACGACG TCTCCAGTAC GCTGAAACGC TTCTTCAGGG AGTTAGAAGA AGGACTGTTC 
ACATCAGCGG ATGCAAGCAG CTGGCTCAGT ACAGCAGGCG TCCAGGATGA AAGTGTGAAA 
ATTGCTCAGT ACCAGCAGCT GCTGAACAGA CTGCCTCATG TGAATAAGGC CACACTACAA 
GCCCTTATCA ACCACCTCTA CTGTGTGCAG CGTTTCTCTG AGCTCAACCA AATGAACCTC 
CACAACCTGG CTATAGTCTT CGGCCCCACA CTGTTCCAGA CAGATGGGAA GGACTACAGC 
GCCGGCCTCA CTGTAGAAGA CCTCATACAG CACTACACAC TTATCTTTGA GGTGGATGAG 
CAGCAGCTAA AGAAGCAGCT GGAAGAGATC ACTGTCATCA TTAAAGTCAG GGAAAAAATG 
AGCACAAAGT TTCCCAGCAC TGAGCCTGGT GGACATTTCA TCTGCACCGT TTACCTGGAG 
GAGAAAAAAG AAACGGCTGA GCAACACGTG AAGATTCCTG GCTCTATGAC AGCAATGGAG 
CTGACGTGTG AGGTCCTTCA TCGACGTAAC ATCCTCGTCA GAGACAAGGA GTACTGGTGC 
TGCTGGGAGA TCAGCAACAA GGAGGAAATG GAGCGTCCAT TGCACTATCA GGAGCGTGTC 
CTACCTATTC TTCACGCCTT AGGCACGGAC TCCCATCTGG TCATAAAGAA GCATCTTGCT 
ATGGAAGCAA TGATCACTTA CCTGTCTAGT AAAGCGGACG CATCCAAACA TGGGATGATG 
AAATTCAGAG AAGAGCGAAG CATCCTGGGA CTGGGACTGA CCTCTGGACA TTTCCATGAC 
CGATACTTCA TCCTCAACTC CAGCTCACTC CGAATGTACA AAGAAATCAG AAGTAACCGC 
TCAGAACGTG ATTGGCCAGT GAAAAACCTC AATGTTTACT TAGGAATCAG GAAAAGACTC 
CGTCCTCCCA CTTGTTGGGG CCTGACGGTG TTATGTGAGA GTAAGAAACC AGATCGACAG 
GAGAAGCAGC AGTTCTATGT TTGCTGTGAC ACCCAGTCAG AAATGCGAGA GTGGTACGCA 
AATTTTCTCA GCGTCCAGTA CAACGGTGAC GTCTGGCCTC AGGACGGTCT GCAGCAGACG 
AGGGTGAGCC GCGTGTTGCC CAACACGCAC CACGGTAACG TTTCACTGAT ACCCCTGCGT 
GGCAGTGAGA ATGAGATGCG GAACAGCGTG GCAGCTTTCA GCCAAGACCC TCTTTCTCTT 
TTTAGGGATG TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910646.1
CDS	372..4169
Translation	MDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHGNVSLIPLRGSENEMRNSVAAFSQDPLSLFRDVP

Target ORF information:

RefSeq Version	XM_025054878.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054878.1

ORF Insert Sequence:

ATGGACAAGG ACCAAGCTCC ACAGGTTCCA CCTCGAATCA GGACACCCCA ATGTCCAGAA 
AGCCTGCAGC AGTCGTCTCA ACTGGACAGT CAGCTGTCTC TGCCTGTGAG AAGAGTCTCC 
CAAACTCCCA GTGAGATCAC TCTAGATTTA CCTGGAAATA ATTCAGATGC AAATGAAGAG 
ACGGACATCC TTCACCTTAA TTCATTAGAC AAGGAAATGC TTGTGCAGGT GCAGAGTCCG 
ACAAAGGGAC ATTACAATTC GTGGTTAAGC GACGACGAGC TGTTGGATGA TGATGATGAT 
GATGATGATG ACGACGATGA TTATGATGAC AATGCTGCTG TGAGTCAGTT CCCGTGGCTT 
GACCGGCCCA TCAGTAACAT TCATGGCAGT GTGTGTCTGC CCAGCACCGG CAGACGGCCT 
TCGGCCGCTA AAAATGACAG CCCCGAGTTG ATTGCCACTG TCATCAAGAC GGGCTGGCTG 
GACAAGAATC CACCACAGGG GGTGCTTTAT TACCAGAGAC GATGGGTGAA ATTGGATGCG 
GATTATCTGC GATACTTTGA CAGTGAAAAG GACATTTATT CCAAGGGAAT CATCTCAACA 
GCCTTCATCA CTAACGTGGC GAGCATAGGA GAGCTAAAGT TTGAGGTGGC TACGAACAAC 
CGGACATTTA TCTTCAGGGC AGAAAATGAA GCTGAGAGAA ACGACTGGGT TGCCGTGCTC 
CTGGACTGCA CCAGAGGGCG CTACCGCCGC AGCAACATCG GCCCCGGTTC ACCCTTGGCC 
CCAGATCATC AGGGGTACCT GGAGCTCAAA GGGTTACGCT CCAAACTTTA CACTGTTGTT 
GCCCTCGATA AAGTCTTTCT CTACAAAACC ATCGATGACT ATCGTATGGG AGTCGGCATC 
ACCTCCATTG ATATGAATGT GGGAAATGTA AAAGATTCGG ACCGTCGTAA CTTCGAACTC 
ACAACGCCTT ACCGCATATT CAGTTTCATA GCGGAGTCGG AGCAGCAGAG GGAACTCTGG 
GTGGCCGCCA TGAGGAACGC TATAGGCGAG GCGCTGTCCA ATAGCGATGT GGCGAACCAG 
ATATGGGCGG AGCCTAGTAA CAGTTTCTGC GCCGACTGTG GGATTGCCAA ACCAGAATGG 
GCAGCTATCA ACTTGTGCGT GGTGGTCTGC AAGCAATGTG CAGGAGAGCA CAGAGGACTG 
GGTCCCAGCG TGTCCAAGGT GCGTAGTCTG AAGATGGACA GGAAGGTTTG GACAGAGGAA 
CTCGTACAGG TGTTTCTCTC GCTAGGCAAC GAGCGGGCCA ACAGCTTTTG GGCTGCTAAC 
GTCCCGCCCA GTGAGGCGCT GGCGCCTTCT AGCAGCAGCG AGGAGAGGAG GCGATTTATC 
ACCAACAAAT ATCGCCAGGG AAAGTACAGA AAGTACCACA TGCTGTATGG GAACCAGACT 
GAACTGAACA ATGCTTTATG TGTGAATATC CAATGCAGTG ATTTGTTGGA GACGTTGAGT 
TTGATCTTCT GCGGGGCAGA CGTAAATTGC TCGACTGGCA TGACAAACTG GCCGTCCCCT 
CTGTCCCTGG CCAACGCACG CTCACAGCAC TTACAGGCTG AAATAATCAG CCACAATCTC 
AACACAGATC AGCCTCAGTC AGAGGGGGTT GTGGCCATGC AGCCAGTTCC TTATTCAAAA 
CCACCTTGTG TGTCTTACAA TGGCTTTCTG TTCAAAACCG CATCCATGGC TCGAGCGGTC 
ACTGAGCGCA AGGCCAGAGA AGAGTTCAGC CGCCGTTGGT GCACGCTGAA CGACGGCGTC 
TTCAGCTATT ATGAGAGCGA CAAGAACTCC AACCCCAATG GAGCACTGAA GGTTTCAGAA 
ATAGTCAGTT TGGCTGTTGA CGCGCCTGAA AAACATGGAT ACGAATACAC CTTTGAAATC 
TACTCGGAGT CAGAGCGACT ATATCTGTTC GGCACCGATG ATGCCGACAG CCACAAAGAA 
TGGGTTAAGT CCATTGCTAG GAGTTTCATA CCCACTGCCG CAGAGCCGTT ACTGAAGATG 
AGTTTTGAGA GGATCGGACG GCTGAAATGT AAAGACGGCT TGAACCTGCA GACGTCTCGG 
CTGGGTTGGT TCGCTCTGGT GGGCTCCACA CTCCATGCCT CACTAGAGGA TGGGCAGTGT 
GAGGAAATCC ACCTCCGTAA AGTTAACGAA CTCTCCACAC AACAGGACAA TGGAGTGCTG 
GTTCTGGTGG AGAAAGGCAG GACTTTGTAC ATAGAAGGTG AGAGAAAGTT GGATTTCGCA 
GGCTGGTGCG CCGCCATCCG ATCGGCAGGA AGGAGCGGAG GAGACACGCT GAGTCAGCAG 
CAGCTCACCG AAACGGACAT ACCTGTCATC GTCCACAGCT GCATTGGATA CATCACTCAG 
TGCGGCTTGG CGTCTGAGGG CATTTATCGC AAAAGCGGCG TGAACTCGCG TGTCAAAGCT 
CTGTGCGAGA GATTTCGGGG TGATGCCCGC AGTCTTTGTC TAAGGGAGGG GGAGCACCAG 
GTGGACGACG TCTCCAGTAC GCTGAAACGC TTCTTCAGGG AGTTAGAAGA AGGACTGTTC 
ACATCAGCGG ATGCAAGCAG CTGGCTCAGT ACAGCAGGCG TCCAGGATGA AAGTGTGAAA 
ATTGCTCAGT ACCAGCAGCT GCTGAACAGA CTGCCTCATG TGAATAAGGC CACACTACAA 
GCCCTTATCA ACCACCTCTA CTGTGTGCAG CGTTTCTCTG AGCTCAACCA AATGAACCTC 
CACAACCTGG CTATAGTCTT CGGCCCCACA CTGTTCCAGA CAGATGGGAA GGACTACAGC 
GCCGGCCTCA CTGTAGAAGA CCTCATACAG CACTACACAC TTATCTTTGA GGTGGATGAG 
CAGCAGCTAA AGAAGCAGCT GGAAGAGATC ACTGTCATCA TTAAAGTCAG GGAAAAAATG 
AGCACAAAGT TTCCCAGCAC TGAGCCTGGT GGACATTTCA TCTGCACCGT TTACCTGGAG 
GAGAAAAAAG AAACGGCTGA GCAACACGTG AAGATTCCTG GCTCTATGAC AGCAATGGAG 
CTGACGTGTG AGGTCCTTCA TCGACGTAAC ATCCTCGTCA GAGACAAGGA GTACTGGTGC 
TGCTGGGAGA TCAGCAACAA GGAGGAAATG GAGCGTCCAT TGCACTATCA GGAGCGTGTC 
CTACCTATTC TTCACGCCTT AGGCACGGAC TCCCATCTGG TCATAAAGAA GCATCTTGCT 
ATGGAAGCAA TGATCACTTA CCTGTCTAGT AAAGCGGACG CATCCAAACA TGGGATGATG 
AAATTCAGAG AAGAGCGAAG CATCCTGGGA CTGGGACTGA CCTCTGGACA TTTCCATGAC 
CGATACTTCA TCCTCAACTC CAGCTCACTC CGAATGTACA AAGAAATCAG AAGTAACCGC 
TCAGAACGTG ATTGGCCAGT GAAAAACCTC AATGTTTACT TAGGAATCAG GAAAAGACTC 
CGTCCTCCCA CTTGTTGGGG CCTGACGGTG TTATGTGAGA GTAAGAAACC AGATCGACAG 
GAGAAGCAGC AGTTCTATGT TTGCTGTGAC ACCCAGTCAG AAATGCGAGA GTGGTACGCA 
AATTTTCTCA GCGTCCAGTA CAACGGTGAC GTCTGGCCTC AGGACGGTCT GCAGCAGACG 
AGGGTGAGCC GCGTGTTGCC CAACACGCAC CACGGTAACG TTTCACTGAT ACCCCTGCGT 
GGCAGTGAGA ATGAGATGCG GAACAGCGTG GCAGCTTTCA GCCAAGACCC TCTTTCTCTT 
TTTAGGGATG TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_025054874.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910642.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy200121
Clone ID Related Accession (Same CDS sequence)	XM_025054874.1 , XM_025054873.1
Accession Version	XM_025054874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGGT AACGTTTCAC TGATACCCCT GCGTGGCAGT 
GAGAATGAGA TGCGGAACAG CGTGGCAGCT TTCAGCCAAG ACCCTCTTTC TCTTTTTAGG 
GATGTTCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910642.1
CDS	211..5022
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHGNVSLIPLRGSENEMRNSVAAFSQDPLSLFRDVP

Target ORF information:

RefSeq Version	XM_025054874.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054874.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGGT AACGTTTCAC TGATACCCCT GCGTGGCAGT 
GAGAATGAGA TGCGGAACAG CGTGGCAGCT TTCAGCCAAG ACCCTCTTTC TCTTTTTAGG 
GATGTTCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_025054873.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910641.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X1, mRNA.

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CloneID	OCy200121
Clone ID Related Accession (Same CDS sequence)	XM_025054874.1 , XM_025054873.1
Accession Version	XM_025054873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGGT AACGTTTCAC TGATACCCCT GCGTGGCAGT 
GAGAATGAGA TGCGGAACAG CGTGGCAGCT TTCAGCCAAG ACCCTCTTTC TCTTTTTAGG 
GATGTTCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910641.1
CDS	210..5021
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHGNVSLIPLRGSENEMRNSVAAFSQDPLSLFRDVP

Target ORF information:

RefSeq Version	XM_025054873.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054873.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGGT AACGTTTCAC TGATACCCCT GCGTGGCAGT 
GAGAATGAGA TGCGGAACAG CGTGGCAGCT TTCAGCCAAG ACCCTCTTTC TCTTTTTAGG 
GATGTTCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_025054875.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910643.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X3, mRNA.

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CloneID	OCy200122
Clone ID Related Accession (Same CDS sequence)	XM_025054875.1
Accession Version	XM_025054875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCACTCTAG ATTTACCTGG AAATAATTCA GATGCAAATG AAGAGACGGA CATCCTTCAC 
CTTAATTCAT TAGACAAGGA AATGCTTGTG CAGGTGCAGA GTCCGACAAA GGGACATTAC 
AATTCGTGGT TAAGCGACGA CGAGCTGTTG GATGATGATG ATGATGATGA TGATGACGAC 
GATGATTATG ATGACAATGC TGCTGTGAGT CAGTTCCCGT GGCTTGACCG GCCCATCAGT 
AACATTCATG GCAGTGTGTG TCTGCCCAGC ACCGGCAGAC GGCCTTCGGC CGCTAAAAAT 
GACAGCCCCG AGTTGATTGC CACTGTCATC AAGACGGGCT GGCTGGACAA GAATCCACCA 
CAGGGGGTGC TTTATTACCA GAGACGATGG GTGAAATTGG ATGCGGATTA TCTGCGATAC 
TTTGACAGTG AAAAGGACAT TTATTCCAAG GGAATCATCT CAACAGCCTT CATCACTAAC 
GTGGCGAGCA TAGGAGAGCT AAAGTTTGAG GTGGCTACGA ACAACCGGAC ATTTATCTTC 
AGGGCAGAAA ATGAAGCTGA GAGAAACGAC TGGGTTGCCG TGCTCCTGGA CTGCACCAGA 
GGGCGCTACC GCCGCAGCAA CATCGGCCCC GGTTCACCCT TGGCCCCAGA TCATCAGGGG 
TACCTGGAGC TCAAAGGGTT ACGCTCCAAA CTTTACACTG TTGTTGCCCT CGATAAAGTC 
TTTCTCTACA AAACCATCGA TGACTATCGT ATGGGAGTCG GCATCACCTC CATTGATATG 
AATGTGGGAA ATGTAAAAGA TTCGGACCGT CGTAACTTCG AACTCACAAC GCCTTACCGC 
ATATTCAGTT TCATAGCGGA GTCGGAGCAG CAGAGGGAAC TCTGGGTGGC CGCCATGAGG 
AACGCTATAG GCGAGGCGCT GTCCAATAGC GATGTGGCGA ACCAGATATG GGCGGAGCCT 
AGTAACAGTT TCTGCGCCGA CTGTGGGATT GCCAAACCAG AATGGGCAGC TATCAACTTG 
TGCGTGGTGG TCTGCAAGCA ATGTGCAGGA GAGCACAGAG GACTGGGTCC CAGCGTGTCC 
AAGGTGCGTA GTCTGAAGAT GGACAGGAAG GTTTGGACAG AGGAACTCGT ACAGGTGTTT 
CTCTCGCTAG GCAACGAGCG GGCCAACAGC TTTTGGGCTG CTAACGTCCC GCCCAGTGAG 
GCGCTGGCGC CTTCTAGCAG CAGCGAGGAG AGGAGGCGAT TTATCACCAA CAAATATCGC 
CAGGGAAAGT ACAGAAAGTA CCACATGCTG TATGGGAACC AGACTGAACT GAACAATGCT 
TTATGTGTGA ATATCCAATG CAGTGATTTG TTGGAGACGT TGAGTTTGAT CTTCTGCGGG 
GCAGACGTAA ATTGCTCGAC TGGCATGACA AACTGGCCGT CCCCTCTGTC CCTGGCCAAC 
GCACGCTCAC AGCACTTACA GGCTGAAATA ATCAGCCACA ATCTCAACAC AGATCAGCCT 
CAGTCAGAGG GGGTTGTGGC CATGCAGCCA GTTCCTTATT CAAAACCACC TTGTGTGTCT 
TACAATGGCT TTCTGTTCAA AACCGCATCC ATGGCTCGAG CGGTCACTGA GCGCAAGGCC 
AGAGAAGAGT TCAGCCGCCG TTGGTGCACG CTGAACGACG GCGTCTTCAG CTATTATGAG 
AGCGACAAGA ACTCCAACCC CAATGGAGCA CTGAAGGTTT CAGAAATAGT CAGTTTGGCT 
GTTGACGCGC CTGAAAAACA TGGATACGAA TACACCTTTG AAATCTACTC GGAGTCAGAG 
CGACTATATC TGTTCGGCAC CGATGATGCC GACAGCCACA AAGAATGGGT TAAGTCCATT 
GCTAGGAGTT TCATACCCAC TGCCGCAGAG CCGTTACTGA AGATGAGTTT TGAGAGGATC 
GGACGGCTGA AATGTAAAGA CGGCTTGAAC CTGCAGACGT CTCGGCTGGG TTGGTTCGCT 
CTGGTGGGCT CCACACTCCA TGCCTCACTA GAGGATGGGC AGTGTGAGGA AATCCACCTC 
CGTAAAGTTA ACGAACTCTC CACACAACAG GACAATGGAG TGCTGGTTCT GGTGGAGAAA 
GGCAGGACTT TGTACATAGA AGGTGAGAGA AAGTTGGATT TCGCAGGCTG GTGCGCCGCC 
ATCCGATCGG CAGGAAGGAG CGGAGGAGAC ACGCTGAGTC AGCAGCAGCT CACCGAAACG 
GACATACCTG TCATCGTCCA CAGCTGCATT GGATACATCA CTCAGTGCGG CTTGGCGTCT 
GAGGGCATTT ATCGCAAAAG CGGCGTGAAC TCGCGTGTCA AAGCTCTGTG CGAGAGATTT 
CGGGGTGATG CCCGCAGTCT TTGTCTAAGG GAGGGGGAGC ACCAGGTGGA CGACGTCTCC 
AGTACGCTGA AACGCTTCTT CAGGGAGTTA GAAGAAGGAC TGTTCACATC AGCGGATGCA 
AGCAGCTGGC TCAGTACAGC AGGCGTCCAG GATGAAAGTG TGAAAATTGC TCAGTACCAG 
CAGCTGCTGA ACAGACTGCC TCATGTGAAT AAGGCCACAC TACAAGCCCT TATCAACCAC 
CTCTACTGTG TGCAGCGTTT CTCTGAGCTC AACCAAATGA ACCTCCACAA CCTGGCTATA 
GTCTTCGGCC CCACACTGTT CCAGACAGAT GGGAAGGACT ACAGCGCCGG CCTCACTGTA 
GAAGACCTCA TACAGCACTA CACACTTATC TTTGAGGTGG ATGAGCAGCA GCTAAAGAAG 
CAGCTGGAAG AGATCACTGT CATCATTAAA GTCAGGGAAA AAATGAGCAC AAAGTTTCCC 
AGCACTGAGC CTGGTGGACA TTTCATCTGC ACCGTTTACC TGGAGGAGAA AAAAGAAACG 
GCTGAGCAAC ACGTGAAGAT TCCTGGCTCT ATGACAGCAA TGGAGCTGAC GTGTGAGGTC 
CTTCATCGAC GTAACATCCT CGTCAGAGAC AAGGAGTACT GGTGCTGCTG GGAGATCAGC 
AACAAGGAGG AAATGGAGCG TCCATTGCAC TATCAGGAGC GTGTCCTACC TATTCTTCAC 
GCCTTAGGCA CGGACTCCCA TCTGGTCATA AAGAAGCATC TTGCTATGGA AGCAATGATC 
ACTTACCTGT CTAGTAAAGC GGACGCATCC AAACATGGGA TGATGAAATT CAGAGAAGAG 
CGAAGCATCC TGGGACTGGG ACTGACCTCT GGACATTTCC ATGACCGATA CTTCATCCTC 
AACTCCAGCT CACTCCGAAT GTACAAAGAA ATCAGAAGTA ACCGCTCAGA ACGTGATTGG 
CCAGTGAAAA ACCTCAATGT TTACTTAGGA ATCAGGAAAA GACTCCGTCC TCCCACTTGT 
TGGGGCCTGA CGGTGTTATG TGAGAGTAAG AAACCAGATC GACAGGAGAA GCAGCAGTTC 
TATGTTTGCT GTGACACCCA GTCAGAAATG CGAGAGTGGT ACGCAAATTT TCTCAGCGTC 
CAGTACAACG GTGACGTCTG GCCTCAGGAC GGTCTGCAGC AGACGAGGGT GAGCCGCGTG 
TTGCCCAACA CGCACCACGG TAACGTTTCA CTGATACCCC TGCGTGGCAG TGAGAATGAG 
ATGCGGAACA GCGTGGCAGC TTTCAGCCAA GACCCTCTTT CTCTTTTTAG GGATGTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910643.1
CDS	213..5015
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPTLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHGNVSLIPLRGSENEMRNSVAAFSQDPLSLFRDVP

Target ORF information:

RefSeq Version	XM_025054875.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054875.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCACTCTAG ATTTACCTGG AAATAATTCA GATGCAAATG AAGAGACGGA CATCCTTCAC 
CTTAATTCAT TAGACAAGGA AATGCTTGTG CAGGTGCAGA GTCCGACAAA GGGACATTAC 
AATTCGTGGT TAAGCGACGA CGAGCTGTTG GATGATGATG ATGATGATGA TGATGACGAC 
GATGATTATG ATGACAATGC TGCTGTGAGT CAGTTCCCGT GGCTTGACCG GCCCATCAGT 
AACATTCATG GCAGTGTGTG TCTGCCCAGC ACCGGCAGAC GGCCTTCGGC CGCTAAAAAT 
GACAGCCCCG AGTTGATTGC CACTGTCATC AAGACGGGCT GGCTGGACAA GAATCCACCA 
CAGGGGGTGC TTTATTACCA GAGACGATGG GTGAAATTGG ATGCGGATTA TCTGCGATAC 
TTTGACAGTG AAAAGGACAT TTATTCCAAG GGAATCATCT CAACAGCCTT CATCACTAAC 
GTGGCGAGCA TAGGAGAGCT AAAGTTTGAG GTGGCTACGA ACAACCGGAC ATTTATCTTC 
AGGGCAGAAA ATGAAGCTGA GAGAAACGAC TGGGTTGCCG TGCTCCTGGA CTGCACCAGA 
GGGCGCTACC GCCGCAGCAA CATCGGCCCC GGTTCACCCT TGGCCCCAGA TCATCAGGGG 
TACCTGGAGC TCAAAGGGTT ACGCTCCAAA CTTTACACTG TTGTTGCCCT CGATAAAGTC 
TTTCTCTACA AAACCATCGA TGACTATCGT ATGGGAGTCG GCATCACCTC CATTGATATG 
AATGTGGGAA ATGTAAAAGA TTCGGACCGT CGTAACTTCG AACTCACAAC GCCTTACCGC 
ATATTCAGTT TCATAGCGGA GTCGGAGCAG CAGAGGGAAC TCTGGGTGGC CGCCATGAGG 
AACGCTATAG GCGAGGCGCT GTCCAATAGC GATGTGGCGA ACCAGATATG GGCGGAGCCT 
AGTAACAGTT TCTGCGCCGA CTGTGGGATT GCCAAACCAG AATGGGCAGC TATCAACTTG 
TGCGTGGTGG TCTGCAAGCA ATGTGCAGGA GAGCACAGAG GACTGGGTCC CAGCGTGTCC 
AAGGTGCGTA GTCTGAAGAT GGACAGGAAG GTTTGGACAG AGGAACTCGT ACAGGTGTTT 
CTCTCGCTAG GCAACGAGCG GGCCAACAGC TTTTGGGCTG CTAACGTCCC GCCCAGTGAG 
GCGCTGGCGC CTTCTAGCAG CAGCGAGGAG AGGAGGCGAT TTATCACCAA CAAATATCGC 
CAGGGAAAGT ACAGAAAGTA CCACATGCTG TATGGGAACC AGACTGAACT GAACAATGCT 
TTATGTGTGA ATATCCAATG CAGTGATTTG TTGGAGACGT TGAGTTTGAT CTTCTGCGGG 
GCAGACGTAA ATTGCTCGAC TGGCATGACA AACTGGCCGT CCCCTCTGTC CCTGGCCAAC 
GCACGCTCAC AGCACTTACA GGCTGAAATA ATCAGCCACA ATCTCAACAC AGATCAGCCT 
CAGTCAGAGG GGGTTGTGGC CATGCAGCCA GTTCCTTATT CAAAACCACC TTGTGTGTCT 
TACAATGGCT TTCTGTTCAA AACCGCATCC ATGGCTCGAG CGGTCACTGA GCGCAAGGCC 
AGAGAAGAGT TCAGCCGCCG TTGGTGCACG CTGAACGACG GCGTCTTCAG CTATTATGAG 
AGCGACAAGA ACTCCAACCC CAATGGAGCA CTGAAGGTTT CAGAAATAGT CAGTTTGGCT 
GTTGACGCGC CTGAAAAACA TGGATACGAA TACACCTTTG AAATCTACTC GGAGTCAGAG 
CGACTATATC TGTTCGGCAC CGATGATGCC GACAGCCACA AAGAATGGGT TAAGTCCATT 
GCTAGGAGTT TCATACCCAC TGCCGCAGAG CCGTTACTGA AGATGAGTTT TGAGAGGATC 
GGACGGCTGA AATGTAAAGA CGGCTTGAAC CTGCAGACGT CTCGGCTGGG TTGGTTCGCT 
CTGGTGGGCT CCACACTCCA TGCCTCACTA GAGGATGGGC AGTGTGAGGA AATCCACCTC 
CGTAAAGTTA ACGAACTCTC CACACAACAG GACAATGGAG TGCTGGTTCT GGTGGAGAAA 
GGCAGGACTT TGTACATAGA AGGTGAGAGA AAGTTGGATT TCGCAGGCTG GTGCGCCGCC 
ATCCGATCGG CAGGAAGGAG CGGAGGAGAC ACGCTGAGTC AGCAGCAGCT CACCGAAACG 
GACATACCTG TCATCGTCCA CAGCTGCATT GGATACATCA CTCAGTGCGG CTTGGCGTCT 
GAGGGCATTT ATCGCAAAAG CGGCGTGAAC TCGCGTGTCA AAGCTCTGTG CGAGAGATTT 
CGGGGTGATG CCCGCAGTCT TTGTCTAAGG GAGGGGGAGC ACCAGGTGGA CGACGTCTCC 
AGTACGCTGA AACGCTTCTT CAGGGAGTTA GAAGAAGGAC TGTTCACATC AGCGGATGCA 
AGCAGCTGGC TCAGTACAGC AGGCGTCCAG GATGAAAGTG TGAAAATTGC TCAGTACCAG 
CAGCTGCTGA ACAGACTGCC TCATGTGAAT AAGGCCACAC TACAAGCCCT TATCAACCAC 
CTCTACTGTG TGCAGCGTTT CTCTGAGCTC AACCAAATGA ACCTCCACAA CCTGGCTATA 
GTCTTCGGCC CCACACTGTT CCAGACAGAT GGGAAGGACT ACAGCGCCGG CCTCACTGTA 
GAAGACCTCA TACAGCACTA CACACTTATC TTTGAGGTGG ATGAGCAGCA GCTAAAGAAG 
CAGCTGGAAG AGATCACTGT CATCATTAAA GTCAGGGAAA AAATGAGCAC AAAGTTTCCC 
AGCACTGAGC CTGGTGGACA TTTCATCTGC ACCGTTTACC TGGAGGAGAA AAAAGAAACG 
GCTGAGCAAC ACGTGAAGAT TCCTGGCTCT ATGACAGCAA TGGAGCTGAC GTGTGAGGTC 
CTTCATCGAC GTAACATCCT CGTCAGAGAC AAGGAGTACT GGTGCTGCTG GGAGATCAGC 
AACAAGGAGG AAATGGAGCG TCCATTGCAC TATCAGGAGC GTGTCCTACC TATTCTTCAC 
GCCTTAGGCA CGGACTCCCA TCTGGTCATA AAGAAGCATC TTGCTATGGA AGCAATGATC 
ACTTACCTGT CTAGTAAAGC GGACGCATCC AAACATGGGA TGATGAAATT CAGAGAAGAG 
CGAAGCATCC TGGGACTGGG ACTGACCTCT GGACATTTCC ATGACCGATA CTTCATCCTC 
AACTCCAGCT CACTCCGAAT GTACAAAGAA ATCAGAAGTA ACCGCTCAGA ACGTGATTGG 
CCAGTGAAAA ACCTCAATGT TTACTTAGGA ATCAGGAAAA GACTCCGTCC TCCCACTTGT 
TGGGGCCTGA CGGTGTTATG TGAGAGTAAG AAACCAGATC GACAGGAGAA GCAGCAGTTC 
TATGTTTGCT GTGACACCCA GTCAGAAATG CGAGAGTGGT ACGCAAATTT TCTCAGCGTC 
CAGTACAACG GTGACGTCTG GCCTCAGGAC GGTCTGCAGC AGACGAGGGT GAGCCGCGTG 
TTGCCCAACA CGCACCACGG TAACGTTTCA CTGATACCCC TGCGTGGCAG TGAGAATGAG 
ATGCGGAACA GCGTGGCAGC TTTCAGCCAA GACCCTCTTT CTCTTTTTAG GGATGTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_025054876.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910644.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X4, mRNA.

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CloneID	OCy200123
Clone ID Related Accession (Same CDS sequence)	XM_025054876.1 , XM_025054877.1
Accession Version	XM_025054876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGCT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910644.1
CDS	215..4930
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHAF

Target ORF information:

RefSeq Version	XM_025054876.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054876.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGCT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_025054877.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024910645.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCy200123
Clone ID Related Accession (Same CDS sequence)	XM_025054876.1 , XM_025054877.1
Accession Version	XM_025054877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGCT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024910645.1
CDS	215..4930
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDDDDDDDYDDNAAVSQFPWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHAF

Target ORF information:

RefSeq Version	XM_025054877.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025054877.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAT 
GATGACGACG ATGATTATGA TGACAATGCT GCTGTGAGTC AGTTCCCGTG GCTTGACCGG 
CCCATCAGTA ACATTCATGG CAGTGTGTGT CTGCCCAGCA CCGGCAGACG GCCTTCGGCC 
GCTAAAAATG ACAGCCCCGA GTTGATTGCC ACTGTCATCA AGACGGGCTG GCTGGACAAG 
AATCCACCAC AGGGGGTGCT TTATTACCAG AGACGATGGG TGAAATTGGA TGCGGATTAT 
CTGCGATACT TTGACAGTGA AAAGGACATT TATTCCAAGG GAATCATCTC AACAGCCTTC 
ATCACTAACG TGGCGAGCAT AGGAGAGCTA AAGTTTGAGG TGGCTACGAA CAACCGGACA 
TTTATCTTCA GGGCAGAAAA TGAAGCTGAG AGAAACGACT GGGTTGCCGT GCTCCTGGAC 
TGCACCAGAG GGCGCTACCG CCGCAGCAAC ATCGGCCCCG GTTCACCCTT GGCCCCAGAT 
CATCAGGGGT ACCTGGAGCT CAAAGGGTTA CGCTCCAAAC TTTACACTGT TGTTGCCCTC 
GATAAAGTCT TTCTCTACAA AACCATCGAT GACTATCGTA TGGGAGTCGG CATCACCTCC 
ATTGATATGA ATGTGGGAAA TGTAAAAGAT TCGGACCGTC GTAACTTCGA ACTCACAACG 
CCTTACCGCA TATTCAGTTT CATAGCGGAG TCGGAGCAGC AGAGGGAACT CTGGGTGGCC 
GCCATGAGGA ACGCTATAGG CGAGGCGCTG TCCAATAGCG ATGTGGCGAA CCAGATATGG 
GCGGAGCCTA GTAACAGTTT CTGCGCCGAC TGTGGGATTG CCAAACCAGA ATGGGCAGCT 
ATCAACTTGT GCGTGGTGGT CTGCAAGCAA TGTGCAGGAG AGCACAGAGG ACTGGGTCCC 
AGCGTGTCCA AGGTGCGTAG TCTGAAGATG GACAGGAAGG TTTGGACAGA GGAACTCGTA 
CAGGTGTTTC TCTCGCTAGG CAACGAGCGG GCCAACAGCT TTTGGGCTGC TAACGTCCCG 
CCCAGTGAGG CGCTGGCGCC TTCTAGCAGC AGCGAGGAGA GGAGGCGATT TATCACCAAC 
AAATATCGCC AGGGAAAGTA CAGAAAGTAC CACATGCTGT ATGGGAACCA GACTGAACTG 
AACAATGCTT TATGTGTGAA TATCCAATGC AGTGATTTGT TGGAGACGTT GAGTTTGATC 
TTCTGCGGGG CAGACGTAAA TTGCTCGACT GGCATGACAA ACTGGCCGTC CCCTCTGTCC 
CTGGCCAACG CACGCTCACA GCACTTACAG GCTGAAATAA TCAGCCACAA TCTCAACACA 
GATCAGCCTC AGTCAGAGGG GGTTGTGGCC ATGCAGCCAG TTCCTTATTC AAAACCACCT 
TGTGTGTCTT ACAATGGCTT TCTGTTCAAA ACCGCATCCA TGGCTCGAGC GGTCACTGAG 
CGCAAGGCCA GAGAAGAGTT CAGCCGCCGT TGGTGCACGC TGAACGACGG CGTCTTCAGC 
TATTATGAGA GCGACAAGAA CTCCAACCCC AATGGAGCAC TGAAGGTTTC AGAAATAGTC 
AGTTTGGCTG TTGACGCGCC TGAAAAACAT GGATACGAAT ACACCTTTGA AATCTACTCG 
GAGTCAGAGC GACTATATCT GTTCGGCACC GATGATGCCG ACAGCCACAA AGAATGGGTT 
AAGTCCATTG CTAGGAGTTT CATACCCACT GCCGCAGAGC CGTTACTGAA GATGAGTTTT 
GAGAGGATCG GACGGCTGAA ATGTAAAGAC GGCTTGAACC TGCAGACGTC TCGGCTGGGT 
TGGTTCGCTC TGGTGGGCTC CACACTCCAT GCCTCACTAG AGGATGGGCA GTGTGAGGAA 
ATCCACCTCC GTAAAGTTAA CGAACTCTCC ACACAACAGG ACAATGGAGT GCTGGTTCTG 
GTGGAGAAAG GCAGGACTTT GTACATAGAA GGTGAGAGAA AGTTGGATTT CGCAGGCTGG 
TGCGCCGCCA TCCGATCGGC AGGAAGGAGC GGAGGAGACA CGCTGAGTCA GCAGCAGCTC 
ACCGAAACGG ACATACCTGT CATCGTCCAC AGCTGCATTG GATACATCAC TCAGTGCGGC 
TTGGCGTCTG AGGGCATTTA TCGCAAAAGC GGCGTGAACT CGCGTGTCAA AGCTCTGTGC 
GAGAGATTTC GGGGTGATGC CCGCAGTCTT TGTCTAAGGG AGGGGGAGCA CCAGGTGGAC 
GACGTCTCCA GTACGCTGAA ACGCTTCTTC AGGGAGTTAG AAGAAGGACT GTTCACATCA 
GCGGATGCAA GCAGCTGGCT CAGTACAGCA GGCGTCCAGG ATGAAAGTGT GAAAATTGCT 
CAGTACCAGC AGCTGCTGAA CAGACTGCCT CATGTGAATA AGGCCACACT ACAAGCCCTT 
ATCAACCACC TCTACTGTGT GCAGCGTTTC TCTGAGCTCA ACCAAATGAA CCTCCACAAC 
CTGGCTATAG TCTTCGGCCC CACACTGTTC CAGACAGATG GGAAGGACTA CAGCGCCGGC 
CTCACTGTAG AAGACCTCAT ACAGCACTAC ACACTTATCT TTGAGGTGGA TGAGCAGCAG 
CTAAAGAAGC AGCTGGAAGA GATCACTGTC ATCATTAAAG TCAGGGAAAA AATGAGCACA 
AAGTTTCCCA GCACTGAGCC TGGTGGACAT TTCATCTGCA CCGTTTACCT GGAGGAGAAA 
AAAGAAACGG CTGAGCAACA CGTGAAGATT CCTGGCTCTA TGACAGCAAT GGAGCTGACG 
TGTGAGGTCC TTCATCGACG TAACATCCTC GTCAGAGACA AGGAGTACTG GTGCTGCTGG 
GAGATCAGCA ACAAGGAGGA AATGGAGCGT CCATTGCACT ATCAGGAGCG TGTCCTACCT 
ATTCTTCACG CCTTAGGCAC GGACTCCCAT CTGGTCATAA AGAAGCATCT TGCTATGGAA 
GCAATGATCA CTTACCTGTC TAGTAAAGCG GACGCATCCA AACATGGGAT GATGAAATTC 
AGAGAAGAGC GAAGCATCCT GGGACTGGGA CTGACCTCTG GACATTTCCA TGACCGATAC 
TTCATCCTCA ACTCCAGCTC ACTCCGAATG TACAAAGAAA TCAGAAGTAA CCGCTCAGAA 
CGTGATTGGC CAGTGAAAAA CCTCAATGTT TACTTAGGAA TCAGGAAAAG ACTCCGTCCT 
CCCACTTGTT GGGGCCTGAC GGTGTTATGT GAGAGTAAGA AACCAGATCG ACAGGAGAAG 
CAGCAGTTCT ATGTTTGCTG TGACACCCAG TCAGAAATGC GAGAGTGGTA CGCAAATTTT 
CTCAGCGTCC AGTACAACGG TGACGTCTGG CCTCAGGACG GTCTGCAGCA GACGAGGGTG 
AGCCGCGTGT TGCCCAACAC GCACCACGCT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arap1 ( XM_017032112.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016887601.1 Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), mRNA.

Price & Availability↑

CloneID	OCy53813
Clone ID Related Accession (Same CDS sequence)	XM_017032112.1
Accession Version	XM_017032112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAG 
TGGCTTGACC GGCCCATCAG TAACATTCAT GGCAGTGTGT GTCTGCCCAG CACCGGCAGA 
CGGCCTTCGG CCGCTAAAAA TGACAGCCCC GAGTTGATTG CCACTGTCAT CAAGACGGGC 
TGGCTGGACA AGAATCCACC ACAGGGGGTG CTTTATTACC AGAGACGATG GGTGAAATTG 
GATGCGGATT ATCTGCGATA CTTTGACAGT GAAAAGGACA TTTATTCCAA GGGAATCATC 
TCAACAGCCT TCATCACTAA CGTGGCGAGC ATAGGAGAGC TAAAGTTTGA GGTGGCTACG 
AACAACCGGA CATTTATCTT CAGGGCAGAA AATGAAGCTG AGAGAAACGA CTGGGTTGCC 
GTGCTCCTGG ACTGCACCAG AGGGCGCTAC CGCCGCAGCA ACATCGGCCC CGGTTCACCC 
TTGGCCCCAG ATCATCAGGG GTACCTGGAG CTCAAAGGGT TACGCTCCAA ACTTTACACT 
GTTGTTGCCC TCGATAAAGT CTTTCTCTAC AAAACCATCG ATGACTATCG TATGGGAGTC 
GGCATCACCT CCATTGATAT GAATGTGGGA AATGTAAAAG ATTCGGACCG TCGTAACTTC 
GAACTCACAA CGCCTTACCG CATATTCAGT TTCATAGCGG AGTCGGAGCA GCAGAGGGAA 
CTCTGGGTGG CCGCCATGAG GAACGCTATA GGCGAGGCGC TGTCCAATAG CGATGTGGCG 
AACCAGATAT GGGCGGAGCC TAGTAACAGT TTCTGCGCCG ACTGTGGGAT TGCCAAACCA 
GAATGGGCAG CTATCAACTT GTGCGTGGTG GTCTGCAAGC AATGTGCAGG AGAGCACAGA 
GGACTGGGTC CCAGCGTGTC CAAGGTGCGT AGTCTGAAGA TGGACAGGAA GGTTTGGACA 
GAGGAACTCG TACAGGTGTT TCTCTCGCTA GGCAACGAGC GGGCCAACAG CTTTTGGGCT 
GCTAACGTCC CGCCCAGTGA GGCGCTGGCG CCTTCTAGCA GCAGCGAGGA GAGGAGGCGA 
TTTATCACCA ACAAATATCG CCAGGGAAAG TACAGAAAGT ACCACATGCT GTATGGGAAC 
CAGACTGAAC TGAACAATGC TTTATGTGTG AATATCCAAT GCAGTGATTT GTTGGAGACG 
TTGAGTTTGA TCTTCTGCGG GGCAGACGTA AATTGCTCGA CTGGCATGAC AAACTGGCCG 
TCCCCTCTGT CCCTGGCCAA CGCACGCTCA CAGCACTTAC AGGCTGAAAT AATCAGCCAC 
AATCTCAACA CAGATCAGCC TCAGTCAGAG GGGGTTGTGG CCATGCAGCC AGTTCCTTAT 
TCAAAACCAC CTTGTGTGTC TTACAATGGC TTTCTGTTCA AAACCGCATC CATGGCTCGA 
GCGGTCACTG AGCGCAAGGC CAGAGAAGAG TTCAGCCGCC GTTGGTGCAC GCTGAACGAC 
GGCGTCTTCA GCTATTATGA GAGCGACAAG AACTCCAACC CCAATGGAGC ACTGAAGGTT 
TCAGAAATAG TCAGTTTGGC TGTTGACGCG CCTGAAAAAC ATGGATACGA ATACACCTTT 
GAAATCTACT CGGAGTCAGA GCGACTATAT CTGTTCGGCA CCGATGATGC CGACAGCCAC 
AAAGAATGGG TTAAGTCCAT TGCTAGGAGT TTCATACCCA CTGCCGCAGA GCCGTTACTG 
AAGATGAGTT TTGAGAGGAT CGGACGGCTG AAATGTAAAG ACGGCTTGAA CCTGCAGACG 
TCTCGGCTGG GTTGGTTCGC TCTGGTGGGC TCCACACTCC ATGCCTCACT AGAGGATGGG 
CAGTGTGAGG AAATCCACCT CCGTAAAGTT AACGAACTCT CCACACAACA GGACAATGGA 
GTGCTGGTTC TGGTGGAGAA AGGCAGGACT TTGTACATAG AAGGTGAGAG AAAGTTGGAT 
TTCGCAGGCT GGTGCGCCGC CATCCGATCG GCAGGAAGGA GCGGAGGAGA CACGCTGAGT 
CAGCAGCAGC TCACCGAAAC GGACATACCT GTCATCGTCC ACAGCTGCAT TGGATACATC 
ACTCAGTGCG GCTTGGCGTC TGAGGGCATT TATCGCAAAA GCGGCGTGAA CTCGCGTGTC 
AAAGCTCTGT GCGAGAGATT TCGGGGTGAT GCCCGCAGTC TTTGTCTAAG GGAGGGGGAG 
CACCAGGTGG ACGACGTCTC CAGTACGCTG AAACGCTTCT TCAGGGAGTT AGAAGAAGGA 
CTGTTCACAT CAGCGGATGC AAGCAGCTGG CTCAGTACAG CAGGCGTCCA GGATGAAAGT 
GTGAAAATTG CTCAGTACCA GCAGCTGCTG AACAGACTGC CTCATGTGAA TAAGGCCACA 
CTACAAGCCC TTATCAACCA CCTCTACTGT GTGCAGCGTT TCTCTGAGCT CAACCAAATG 
AACCTCCACA ACCTGGCTAT AGTCTTCGGC CCCACACTGT TCCAGACAGA TGGGAAGGAC 
TACAGCGCCG GCCTCACTGT AGAAGACCTC ATACAGCACT ACACACTTAT CTTTGAGGTG 
GATGAGCAGC AGCTAAAGAA GCAGCTGGAA GAGATCACTG TCATCATTAA AGTCAGGGAA 
AAAATGAGCA CAAAGTTTCC CAGCACTGAG CCTGGTGGAC ATTTCATCTG CACCGTTTAC 
CTGGAGGAGA AAAAAGAAAC GGCTGAGCAA CACGTGAAGA TTCCTGGCTC TATGACAGCA 
ATGGAGCTGA CGTGTGAGGT CCTTCATCGA CGTAACATCC TCGTCAGAGA CAAGGAGTAC 
TGGTGCTGCT GGGAGATCAG CAACAAGGAG GAAATGGAGC GTCCATTGCA CTATCAGGAG 
CGTGTCCTAC CTATTCTTCA CGCCTTAGGC ACGGACTCCC ATCTGGTCAT AAAGAAGCAT 
CTTGCTATGG AAGCAATGAT CACTTACCTG TCTAGTAAAG CGGACGCATC CAAACATGGG 
ATGATGAAAT TCAGAGAAGA GCGAAGCATC CTGGGACTGG GACTGACCTC TGGACATTTC 
CATGACCGAT ACTTCATCCT CAACTCCAGC TCACTCCGAA TGTACAAAGA AATCAGAAGT 
AACCGCTCAG AACGTGATTG GCCAGTGAAA AACCTCAATG TTTACTTAGG AATCAGGAAA 
AGACTCCGTC CTCCCACTTG TTGGGGCCTG ACGGTGTTAT GTGAGAGTAA GAAACCAGAT 
CGACAGGAGA AGCAGCAGTT CTATGTTTGC TGTGACACCC AGTCAGAAAT GCGAGAGTGG 
TACGCAAATT TTCTCAGCGT CCAGTACAAC GGTGACGTCT GGCCTCAGGA CGGTCTGCAG 
CAGACGAGGG TGAGCCGCGT GTTGCCCAAC ACGCACCACG GTAACGTTTC ACTGATACCC 
CTGCGTGGCA GTGAGAATGA GATGCGGAAC AGCGTGGCAG CTTTCAGCCA AGACCCTCTT 
TCTCTTTTTA GGGATGTTCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016887601.1
CDS	154..4917
Translation	MDVDLPSPMPRSHPMSHTDPGANVWDWLSVLRLDQYTVAFQNAGLMTLQQCRNLTQDQLGSIGVTLPGHQRRILASLNKTQGSHDTQSVTHSPPGLSERTLQSEDVDSADELQKEKYLLSSERPLEKRDTEMPRPAPRERTTPVPKEKQPTSQEDHGETDVERERPVPRERTKFSNNVVVKCPPFPASSSTSPLDAPLPPVPPRSTTNCPPQPFLPSLSLIPPPKTPGTPAQDRHDVQGPAAQKAPHDATSACTSTGPAARERPQTLDIQPHCQYLHPEGARKTSPVSPASASISSDRNAPPLPPKLSAGSKGPPLIPCRVSTPSKTNSLSPAETSEEMDKDQAPQVPPRIRTPQCPESLQQSSQLDSQLSLPVRRVSQTPSEITLDLPGNNSDANEETDILHLNSLDKEMLVQVQSPTKGHYNSWLSDDELLDDDDDDEWLDRPISNIHGSVCLPSTGRRPSAAKNDSPELIATVIKTGWLDKNPPQGVLYYQRRWVKLDADYLRYFDSEKDIYSKGIISTAFITNVASIGELKFEVATNNRTFIFRAENEAERNDWVAVLLDCTRGRYRRSNIGPGSPLAPDHQGYLELKGLRSKLYTVVALDKVFLYKTIDDYRMGVGITSIDMNVGNVKDSDRRNFELTTPYRIFSFIAESEQQRELWVAAMRNAIGEALSNSDVANQIWAEPSNSFCADCGIAKPEWAAINLCVVVCKQCAGEHRGLGPSVSKVRSLKMDRKVWTEELVQVFLSLGNERANSFWAANVPPSEALAPSSSSEERRRFITNKYRQGKYRKYHMLYGNQTELNNALCVNIQCSDLLETLSLIFCGADVNCSTGMTNWPSPLSLANARSQHLQAEIISHNLNTDQPQSEGVVAMQPVPYSKPPCVSYNGFLFKTASMARAVTERKAREEFSRRWCTLNDGVFSYYESDKNSNPNGALKVSEIVSLAVDAPEKHGYEYTFEIYSESERLYLFGTDDADSHKEWVKSIARSFIPTAAEPLLKMSFERIGRLKCKDGLNLQTSRLGWFALVGSTLHASLEDGQCEEIHLRKVNELSTQQDNGVLVLVEKGRTLYIEGERKLDFAGWCAAIRSAGRSGGDTLSQQQLTETDIPVIVHSCIGYITQCGLASEGIYRKSGVNSRVKALCERFRGDARSLCLREGEHQVDDVSSTLKRFFRELEEGLFTSADASSWLSTAGVQDESVKIAQYQQLLNRLPHVNKATLQALINHLYCVQRFSELNQMNLHNLAIVFGPTLFQTDGKDYSAGLTVEDLIQHYTLIFEVDEQQLKKQLEEITVIIKVREKMSTKFPSTEPGGHFICTVYLEEKKETAEQHVKIPGSMTAMELTCEVLHRRNILVRDKEYWCCWEISNKEEMERPLHYQERVLPILHALGTDSHLVIKKHLAMEAMITYLSSKADASKHGMMKFREERSILGLGLTSGHFHDRYFILNSSSLRMYKEIRSNRSERDWPVKNLNVYLGIRKRLRPPTCWGLTVLCESKKPDRQEKQQFYVCCDTQSEMREWYANFLSVQYNGDVWPQDGLQQTRVSRVLPNTHHGNVSLIPLRGSENEMRNSVAAFSQDPLSLFRDVP

Target ORF information:

RefSeq Version	XM_017032112.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (arap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017032112.1

ORF Insert Sequence:

ATGGACGTGG ACTTGCCCTC CCCCATGCCA CGCTCTCATC CCATGTCACA CACTGATCCC 
GGCGCAAATG TGTGGGACTG GCTGTCTGTT CTTCGCCTGG ACCAGTACAC CGTGGCATTT 
CAGAACGCTG GACTTATGAC ACTACAGCAG TGTCGCAACC TCACACAAGA TCAGCTGGGG 
AGCATTGGTG TCACCCTGCC GGGTCACCAA AGACGCATCT TGGCCAGTCT GAACAAAACA 
CAGGGAAGTC ATGACACACA GTCTGTCACA CACTCTCCTC CTGGCCTGTC TGAAAGGACC 
CTCCAATCGG AGGATGTTGA CTCAGCGGAT GAGTTACAGA AAGAGAAATA TTTGCTCTCT 
TCTGAGAGAC CTCTGGAAAA AAGAGACACT GAGATGCCGA GACCTGCTCC CAGAGAAAGA 
ACTACACCTG TGCCCAAGGA AAAGCAACCA ACTTCACAAG AGGATCACGG AGAAACGGAC 
GTAGAGAGGG AGAGACCTGT GCCCAGAGAG AGGACGAAAT TTAGCAATAA CGTTGTAGTA 
AAATGCCCTC CTTTTCCAGC GTCCTCCTCG ACTTCACCTC TCGACGCTCC TCTGCCCCCC 
GTGCCTCCTC GAAGCACCAC CAACTGCCCT CCGCAGCCCT TTTTGCCCAG CCTGAGCCTA 
ATTCCTCCTC CGAAAACTCC TGGCACTCCT GCACAAGACA GACATGATGT CCAAGGTCCA 
GCTGCGCAGA AAGCGCCTCA TGATGCAACC TCCGCCTGCA CTTCCACCGG TCCTGCTGCC 
AGAGAACGTC CACAAACATT AGACATCCAG CCACATTGCC AGTATCTCCA CCCCGAGGGA 
GCAAGAAAAA CATCACCCGT GTCCCCCGCG TCGGCTTCCA TCAGCAGCGA CAGAAATGCT 
CCTCCTCTTC CTCCAAAACT CAGCGCAGGA TCCAAAGGCC CACCGCTGAT CCCTTGCAGG 
GTTTCCACGC CTTCCAAAAC GAACAGCCTC TCACCCGCGG AGACCTCTGA AGAGATGGAC 
AAGGACCAAG CTCCACAGGT TCCACCTCGA ATCAGGACAC CCCAATGTCC AGAAAGCCTG 
CAGCAGTCGT CTCAACTGGA CAGTCAGCTG TCTCTGCCTG TGAGAAGAGT CTCCCAAACT 
CCCAGTGAGA TCACTCTAGA TTTACCTGGA AATAATTCAG ATGCAAATGA AGAGACGGAC 
ATCCTTCACC TTAATTCATT AGACAAGGAA ATGCTTGTGC AGGTGCAGAG TCCGACAAAG 
GGACATTACA ATTCGTGGTT AAGCGACGAC GAGCTGTTGG ATGATGATGA TGATGATGAG 
TGGCTTGACC GGCCCATCAG TAACATTCAT GGCAGTGTGT GTCTGCCCAG CACCGGCAGA 
CGGCCTTCGG CCGCTAAAAA TGACAGCCCC GAGTTGATTG CCACTGTCAT CAAGACGGGC 
TGGCTGGACA AGAATCCACC ACAGGGGGTG CTTTATTACC AGAGACGATG GGTGAAATTG 
GATGCGGATT ATCTGCGATA CTTTGACAGT GAAAAGGACA TTTATTCCAA GGGAATCATC 
TCAACAGCCT TCATCACTAA CGTGGCGAGC ATAGGAGAGC TAAAGTTTGA GGTGGCTACG 
AACAACCGGA CATTTATCTT CAGGGCAGAA AATGAAGCTG AGAGAAACGA CTGGGTTGCC 
GTGCTCCTGG ACTGCACCAG AGGGCGCTAC CGCCGCAGCA ACATCGGCCC CGGTTCACCC 
TTGGCCCCAG ATCATCAGGG GTACCTGGAG CTCAAAGGGT TACGCTCCAA ACTTTACACT 
GTTGTTGCCC TCGATAAAGT CTTTCTCTAC AAAACCATCG ATGACTATCG TATGGGAGTC 
GGCATCACCT CCATTGATAT GAATGTGGGA AATGTAAAAG ATTCGGACCG TCGTAACTTC 
GAACTCACAA CGCCTTACCG CATATTCAGT TTCATAGCGG AGTCGGAGCA GCAGAGGGAA 
CTCTGGGTGG CCGCCATGAG GAACGCTATA GGCGAGGCGC TGTCCAATAG CGATGTGGCG 
AACCAGATAT GGGCGGAGCC TAGTAACAGT TTCTGCGCCG ACTGTGGGAT TGCCAAACCA 
GAATGGGCAG CTATCAACTT GTGCGTGGTG GTCTGCAAGC AATGTGCAGG AGAGCACAGA 
GGACTGGGTC CCAGCGTGTC CAAGGTGCGT AGTCTGAAGA TGGACAGGAA GGTTTGGACA 
GAGGAACTCG TACAGGTGTT TCTCTCGCTA GGCAACGAGC GGGCCAACAG CTTTTGGGCT 
GCTAACGTCC CGCCCAGTGA GGCGCTGGCG CCTTCTAGCA GCAGCGAGGA GAGGAGGCGA 
TTTATCACCA ACAAATATCG CCAGGGAAAG TACAGAAAGT ACCACATGCT GTATGGGAAC 
CAGACTGAAC TGAACAATGC TTTATGTGTG AATATCCAAT GCAGTGATTT GTTGGAGACG 
TTGAGTTTGA TCTTCTGCGG GGCAGACGTA AATTGCTCGA CTGGCATGAC AAACTGGCCG 
TCCCCTCTGT CCCTGGCCAA CGCACGCTCA CAGCACTTAC AGGCTGAAAT AATCAGCCAC 
AATCTCAACA CAGATCAGCC TCAGTCAGAG GGGGTTGTGG CCATGCAGCC AGTTCCTTAT 
TCAAAACCAC CTTGTGTGTC TTACAATGGC TTTCTGTTCA AAACCGCATC CATGGCTCGA 
GCGGTCACTG AGCGCAAGGC CAGAGAAGAG TTCAGCCGCC GTTGGTGCAC GCTGAACGAC 
GGCGTCTTCA GCTATTATGA GAGCGACAAG AACTCCAACC CCAATGGAGC ACTGAAGGTT 
TCAGAAATAG TCAGTTTGGC TGTTGACGCG CCTGAAAAAC ATGGATACGA ATACACCTTT 
GAAATCTACT CGGAGTCAGA GCGACTATAT CTGTTCGGCA CCGATGATGC CGACAGCCAC 
AAAGAATGGG TTAAGTCCAT TGCTAGGAGT TTCATACCCA CTGCCGCAGA GCCGTTACTG 
AAGATGAGTT TTGAGAGGAT CGGACGGCTG AAATGTAAAG ACGGCTTGAA CCTGCAGACG 
TCTCGGCTGG GTTGGTTCGC TCTGGTGGGC TCCACACTCC ATGCCTCACT AGAGGATGGG 
CAGTGTGAGG AAATCCACCT CCGTAAAGTT AACGAACTCT CCACACAACA GGACAATGGA 
GTGCTGGTTC TGGTGGAGAA AGGCAGGACT TTGTACATAG AAGGTGAGAG AAAGTTGGAT 
TTCGCAGGCT GGTGCGCCGC CATCCGATCG GCAGGAAGGA GCGGAGGAGA CACGCTGAGT 
CAGCAGCAGC TCACCGAAAC GGACATACCT GTCATCGTCC ACAGCTGCAT TGGATACATC 
ACTCAGTGCG GCTTGGCGTC TGAGGGCATT TATCGCAAAA GCGGCGTGAA CTCGCGTGTC 
AAAGCTCTGT GCGAGAGATT TCGGGGTGAT GCCCGCAGTC TTTGTCTAAG GGAGGGGGAG 
CACCAGGTGG ACGACGTCTC CAGTACGCTG AAACGCTTCT TCAGGGAGTT AGAAGAAGGA 
CTGTTCACAT CAGCGGATGC AAGCAGCTGG CTCAGTACAG CAGGCGTCCA GGATGAAAGT 
GTGAAAATTG CTCAGTACCA GCAGCTGCTG AACAGACTGC CTCATGTGAA TAAGGCCACA 
CTACAAGCCC TTATCAACCA CCTCTACTGT GTGCAGCGTT TCTCTGAGCT CAACCAAATG 
AACCTCCACA ACCTGGCTAT AGTCTTCGGC CCCACACTGT TCCAGACAGA TGGGAAGGAC 
TACAGCGCCG GCCTCACTGT AGAAGACCTC ATACAGCACT ACACACTTAT CTTTGAGGTG 
GATGAGCAGC AGCTAAAGAA GCAGCTGGAA GAGATCACTG TCATCATTAA AGTCAGGGAA 
AAAATGAGCA CAAAGTTTCC CAGCACTGAG CCTGGTGGAC ATTTCATCTG CACCGTTTAC 
CTGGAGGAGA AAAAAGAAAC GGCTGAGCAA CACGTGAAGA TTCCTGGCTC TATGACAGCA 
ATGGAGCTGA CGTGTGAGGT CCTTCATCGA CGTAACATCC TCGTCAGAGA CAAGGAGTAC 
TGGTGCTGCT GGGAGATCAG CAACAAGGAG GAAATGGAGC GTCCATTGCA CTATCAGGAG 
CGTGTCCTAC CTATTCTTCA CGCCTTAGGC ACGGACTCCC ATCTGGTCAT AAAGAAGCAT 
CTTGCTATGG AAGCAATGAT CACTTACCTG TCTAGTAAAG CGGACGCATC CAAACATGGG 
ATGATGAAAT TCAGAGAAGA GCGAAGCATC CTGGGACTGG GACTGACCTC TGGACATTTC 
CATGACCGAT ACTTCATCCT CAACTCCAGC TCACTCCGAA TGTACAAAGA AATCAGAAGT 
AACCGCTCAG AACGTGATTG GCCAGTGAAA AACCTCAATG TTTACTTAGG AATCAGGAAA 
AGACTCCGTC CTCCCACTTG TTGGGGCCTG ACGGTGTTAT GTGAGAGTAA GAAACCAGAT 
CGACAGGAGA AGCAGCAGTT CTATGTTTGC TGTGACACCC AGTCAGAAAT GCGAGAGTGG 
TACGCAAATT TTCTCAGCGT CCAGTACAAC GGTGACGTCT GGCCTCAGGA CGGTCTGCAG 
CAGACGAGGG TGAGCCGCGT GTTGCCCAAC ACGCACCACG GTAACGTTTC ACTGATACCC 
CTGCGTGGCA GTGAGAATGA GATGCGGAAC AGCGTGGCAG CTTTCAGCCA AGACCCTCTT 
TCTCTTTTTA GGGATGTTCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

arap1 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム