VP cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	VP
Entrez Gene ID	101222673
Full Name	pyrophosphate-energized vacuolar membrane proton pump
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308846.1	NP_001295775.1	pyrophosphate-energized vacuolar membrane proton pump

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
Li Z, Zhang Z, Yan P, Huang S, Fei Z, Lin K
BMC genomics12540(2011 Nov)

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
Guo S, Zheng Y, Joung JG, Liu S, Zhang Z, Crasta OR, Sobral BW, Xu Y, Huang S, Fei Z
BMC genomics11384(2010 Jun)

The genome of the cucumber, Cucumis sativus L.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EA, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, Du Y, Li S
Nature genetics41(12)1275-81(2009 Dec)

An integrated genetic and cytogenetic map of the cucumber genome.
Ren Y, Zhang Z, Liu J, Staub JE, Han Y, Cheng Z, Li X, Lu J, Miao H, Kang H, Xie B, Gu X, Wang X, Du Y, Jin W, Huang S
PloS one4(6)e5795(2009 Jun)

Modification of vacuolar proton pumps in cucumber roots under salt stress.
Kaba?a K, K?obus G
Journal of plant physiology165(17)1830-7(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu03748	NM_001308846.1 Latest version!	Cucumis sativus pyrophosphate-energized vacuolar membrane proton pump (VP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VP ( NM_001308846.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001295775.1 Cucumis sativus pyrophosphate-energized vacuolar membrane proton pump (VP), mRNA.

Price & Availability↑

CloneID	OCu03748
Clone ID Related Accession (Same CDS sequence)	NM_001308846.1
Accession Version	NM_001308846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	pyrophosphate-energized vacuolar membrane proton pump
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ223786.1. On May 16, 2015 this sequence version replaced XM_004150458.2. ##Evidence-Data-START## Transcript exon combination :: GQ223786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01085195, SAMN01085196 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCGG CGATTCTGCC AGATCTCGGT GCTCAGATTT TCATTCCTCT CTGTGCTATT 
GTCGGAATCC TCTTCTCTTT AGTCCAGTGG TACTACGTTT CACAAGTTAA ACTCTCTTCT 
GCTCGTGATT CCGCTAATAA CAATTCCTCT TCTGCTAAAA ATGGCTACTC TGACTACCTT 
ATTGAAGAGG AAGAAGGTGT TAATGACCAT AACGTTGTTA TTAAATGTGC CGAAATTCAA 
AACGCCATCT CTGAAGGGGC AACCTCCTTC CTTTTCACGG AGTACAAGTA TGTTGGCATC 
TTTATGATAT TGTTTGCAGC ATTGATTTTT GTATTCCTTG GATCAGTAGA GGGTTTTAGT 
ACCAAGCCCC AACCATGCTC ATATGACAAA ACAAAGACTT GTAAGCCAGC TTTGGCAACG 
GCTACATTCA GTACTATATC ATTTTTGCTT GGTGCAGTGA CTTCAGTGGT TTCTGGTTTC 
CTTGGAATGA AAATTGCTAC ATATGCAAAT GCCAGAACCA CCTTGGAGGC AAGAAAAGGT 
GTTGGAAAGG CATTTATTAC TGCTTTTAGG TCTGGTGCTG TCATGGGCTT CCTCCTAGCT 
GCCAACGGTC TCTTGGTTTT GTTTATTGCC ATTAACCTCT TCAAATTGTA CTATGGTGAA 
GATTGGGGTG GTCTTTTCGA GTCTATCACT GGGTATGGTC TTGGTGGATC CTCCATGGCT 
CTCTTTGGTA GAGTTGGTGG TGGTATCTAC ACCAAAGCTG CTGATGTTGG TGCCGACCTT 
GTGGGTAAAG TGGAAAGAAA CATTCCTGAG GACGACCCAA GAAATCCAGC TGTGATTGCT 
GATAATGTTG GTGACAATGT TGGGGATATT GCCGGTATGG GATCTGATCT TTTTGGTTCA 
TATGCTGAGT CATCCTGTGC TGCTCTCGTT GTTGCATCTA TCTCCTCTTT CGGCAACAAC 
CATGAGTTCA CAGCAATGCT CTATCCACTC ATCGTTAGTT CTATGGGTAT CCTTGTCTGT 
CTGATCACCA CTTTATTTGC AACGGATTTC TTTGAGATCA AGGCTGTTAA GGAAATTGAG 
CCAGCATTGA AGAATCAACT CATAATTTCC ACTGTTATAA TGACCTTTGG AATTGCAATT 
GTTACTTGGG TGAGTGTTCC AGCAAAATTT ACCATCTTCA ACTTTGGAAC TCAAAAAGTT 
GTACAGAACT GGGAACTTTT CTTGTGCGTT GCTGTTGGTC TTTGGGCTGG GCTGATAATA 
GGATTTGTGA CAGAGTACTA TACTAGCAAT GCATACAGCC CTGTGCAAGA CGTTGCTGAT 
TCTTGCCGAA CTGGAGCTGC TACAAATGTT ATTTTTGGCT TGGCCTTGGG ATACAAATCT 
GTCATTATCC CTATCTTTGC AATTGCAGCT AGTATTTTTG TGAGTTTCTC CTTTGCTGCA 
ATGTACGGCA TTGCCGTTGC TGCCCTTGGA ATGTTGAGTA CAATTGCTAC TGGTTTGGCC 
ATCGATGCAT ATGGTCCAAT CAGTGACAAT GCTGGAGGCA TTGCTGAGAT GGCTGGCATG 
AGCCACAGAA TTCGGGAGAG AACCGATGCC CTTGATGCTG CTGGAAACAC GACTGCAGCA 
ATTGGAAAGG GTTTTGCCAT TGGTTCAGCT GCTCTCGTGT CCCTTGCCCT CTTTGGTGCC 
TTTGTTAGCC GTGCTGGTGT TGTCGTTCTT GACCTCTTGA CACCCAAAGT TTTCATTGGC 
TTGATCGTTG GTGCTATGCT GCCATACTGG TTTTCTGCCA TGACAATGAA GAGTGTTGGG 
AGTGCAGCCC TAAAGATGGT TGAGGAGGTC CGAAGGCAAT TCAACACAAT CCCAGGTCTC 
ATGGAAGGTA CTGCCAAGCC CGACTATGCT ACGTGTGTCA AGATCTCAAC TGATGCATCT 
ATTAAGGAGA TGATCCCACC TGGTGCCCTT GTCATGTTGA CACCCCTCAT TGTTGGTATC 
CTATTCGGAG TTGAAACTCT TTCTGGCGTC CTTGCTGGAT CCCTTGTTTC TGGTGTCCAG 
ATTGCCATTT CCGCATCCAA CACCGGAGGT GCTTGGGACA ATGCTAAGAA GTACATCGAG 
GCTGGCACCT CTGAGCACGC AAGAACCCTT GGCCCGAAAG GATCAGATCC ACACAAAACT 
GCTGTTATCG GGGACACAAT CGGAGACCCA CTCAAGGATA CATCTGGACC TTCTCTCAAC 
ATTCTCATCA AGTTGATGGC TGTGGAGTCA CTCGTGTTTG CTCCTTTCTT TGCCAGCCAC 
GGTGGCATTC TGTTCAAGAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295775.1
CDS	1..2307
Translation	MGAAILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVVVLDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKTAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF

Target ORF information:

RefSeq Version	NM_001308846.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus pyrophosphate-energized vacuolar membrane proton pump (VP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308846.1

ORF Insert Sequence:

ATGGGCGCGG CGATTCTGCC AGATCTCGGT GCTCAGATTT TCATTCCTCT CTGTGCTATT 
GTCGGAATCC TCTTCTCTTT AGTCCAGTGG TACTACGTTT CACAAGTTAA ACTCTCTTCT 
GCTCGTGATT CCGCTAATAA CAATTCCTCT TCTGCTAAAA ATGGCTACTC TGACTACCTT 
ATTGAAGAGG AAGAAGGTGT TAATGACCAT AACGTTGTTA TTAAATGTGC CGAAATTCAA 
AACGCCATCT CTGAAGGGGC AACCTCCTTC CTTTTCACGG AGTACAAGTA TGTTGGCATC 
TTTATGATAT TGTTTGCAGC ATTGATTTTT GTATTCCTTG GATCAGTAGA GGGTTTTAGT 
ACCAAGCCCC AACCATGCTC ATATGACAAA ACAAAGACTT GTAAGCCAGC TTTGGCAACG 
GCTACATTCA GTACTATATC ATTTTTGCTT GGTGCAGTGA CTTCAGTGGT TTCTGGTTTC 
CTTGGAATGA AAATTGCTAC ATATGCAAAT GCCAGAACCA CCTTGGAGGC AAGAAAAGGT 
GTTGGAAAGG CATTTATTAC TGCTTTTAGG TCTGGTGCTG TCATGGGCTT CCTCCTAGCT 
GCCAACGGTC TCTTGGTTTT GTTTATTGCC ATTAACCTCT TCAAATTGTA CTATGGTGAA 
GATTGGGGTG GTCTTTTCGA GTCTATCACT GGGTATGGTC TTGGTGGATC CTCCATGGCT 
CTCTTTGGTA GAGTTGGTGG TGGTATCTAC ACCAAAGCTG CTGATGTTGG TGCCGACCTT 
GTGGGTAAAG TGGAAAGAAA CATTCCTGAG GACGACCCAA GAAATCCAGC TGTGATTGCT 
GATAATGTTG GTGACAATGT TGGGGATATT GCCGGTATGG GATCTGATCT TTTTGGTTCA 
TATGCTGAGT CATCCTGTGC TGCTCTCGTT GTTGCATCTA TCTCCTCTTT CGGCAACAAC 
CATGAGTTCA CAGCAATGCT CTATCCACTC ATCGTTAGTT CTATGGGTAT CCTTGTCTGT 
CTGATCACCA CTTTATTTGC AACGGATTTC TTTGAGATCA AGGCTGTTAA GGAAATTGAG 
CCAGCATTGA AGAATCAACT CATAATTTCC ACTGTTATAA TGACCTTTGG AATTGCAATT 
GTTACTTGGG TGAGTGTTCC AGCAAAATTT ACCATCTTCA ACTTTGGAAC TCAAAAAGTT 
GTACAGAACT GGGAACTTTT CTTGTGCGTT GCTGTTGGTC TTTGGGCTGG GCTGATAATA 
GGATTTGTGA CAGAGTACTA TACTAGCAAT GCATACAGCC CTGTGCAAGA CGTTGCTGAT 
TCTTGCCGAA CTGGAGCTGC TACAAATGTT ATTTTTGGCT TGGCCTTGGG ATACAAATCT 
GTCATTATCC CTATCTTTGC AATTGCAGCT AGTATTTTTG TGAGTTTCTC CTTTGCTGCA 
ATGTACGGCA TTGCCGTTGC TGCCCTTGGA ATGTTGAGTA CAATTGCTAC TGGTTTGGCC 
ATCGATGCAT ATGGTCCAAT CAGTGACAAT GCTGGAGGCA TTGCTGAGAT GGCTGGCATG 
AGCCACAGAA TTCGGGAGAG AACCGATGCC CTTGATGCTG CTGGAAACAC GACTGCAGCA 
ATTGGAAAGG GTTTTGCCAT TGGTTCAGCT GCTCTCGTGT CCCTTGCCCT CTTTGGTGCC 
TTTGTTAGCC GTGCTGGTGT TGTCGTTCTT GACCTCTTGA CACCCAAAGT TTTCATTGGC 
TTGATCGTTG GTGCTATGCT GCCATACTGG TTTTCTGCCA TGACAATGAA GAGTGTTGGG 
AGTGCAGCCC TAAAGATGGT TGAGGAGGTC CGAAGGCAAT TCAACACAAT CCCAGGTCTC 
ATGGAAGGTA CTGCCAAGCC CGACTATGCT ACGTGTGTCA AGATCTCAAC TGATGCATCT 
ATTAAGGAGA TGATCCCACC TGGTGCCCTT GTCATGTTGA CACCCCTCAT TGTTGGTATC 
CTATTCGGAG TTGAAACTCT TTCTGGCGTC CTTGCTGGAT CCCTTGTTTC TGGTGTCCAG 
ATTGCCATTT CCGCATCCAA CACCGGAGGT GCTTGGGACA ATGCTAAGAA GTACATCGAG 
GCTGGCACCT CTGAGCACGC AAGAACCCTT GGCCCGAAAG GATCAGATCC ACACAAAACT 
GCTGTTATCG GGGACACAAT CGGAGACCCA CTCAAGGATA CATCTGGACC TTCTCTCAAC 
ATTCTCATCA AGTTGATGGC TGTGGAGTCA CTCGTGTTTG CTCCTTTCTT TGCCAGCCAC 
GGTGGCATTC TGTTCAAGAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
BMC genomics12540(2011 Nov)
Li Z,Zhang Z,Yan P,Huang S,Fei Z,Lin K

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
BMC genomics11384(2010 Jun)
Guo S,Zheng Y,Joung JG,Liu S,Zhang Z,Crasta OR,Sobral BW,Xu Y,Huang S,Fei Z

The genome of the cucumber, Cucumis sativus L.
Nature genetics41(12)1275-81(2009 Dec)
Huang S,Li R,Zhang Z,Li L,Gu X,Fan W,Lucas WJ,Wang X,Xie B,Ni P,Ren Y,Zhu H,Li J,Lin K,Jin W,Fei Z,Li G,Staub J,Kilian A,van der Vossen EA,Wu Y,Guo J,He J,Jia Z,Ren Y,Tian G,Lu Y,Ruan J,Qian W,Wang M,Huang Q,Li B,Xuan Z,Cao J,Asan,Wu Z,Zhang J,Cai Q,Bai Y,Zhao B,Han Y,Li Y,Li X,Wang S,Shi Q,Liu S,Cho WK,Kim JY,Xu Y,Heller-Uszynska K,Miao H,Cheng Z,Zhang S,Wu J,Yang Y,Kang H,Li M,Liang H,Ren X,Shi Z,Wen M,Jian M,Yang H,Zhang G,Yang Z,Chen R,Liu S,Li J,Ma L,Liu H,Zhou Y,Zhao J,Fang X,Li G,Fang L,Li Y,Liu D,Zheng H,Zhang Y,Qin N,Li Z,Yang G,Yang S,Bolund L,Kristiansen K,Zheng H,Li S,Zhang X,Yang H,Wang J,Sun R,Zhang B,Jiang S,Wang J,Du Y,Li S

An integrated genetic and cytogenetic map of the cucumber genome.
PloS one4(6)e5795(2009 Jun)
Ren Y,Zhang Z,Liu J,Staub JE,Han Y,Cheng Z,Li X,Lu J,Miao H,Kang H,Xie B,Gu X,Wang X,Du Y,Jin W,Huang S

Modification of vacuolar proton pumps in cucumber roots under salt stress.
Journal of plant physiology165(17)1830-7(2008 Nov)
Kaba?a K,K?obus G