HA3 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	HA3
Entrez Gene ID	101221783
Full Name	plasma membrane ATPase 4
Synonyms	ha1
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305750.1	NP_001292679.1	plasma membrane ATPase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots.
Janicka-Russak M, Kaba?a K, Burzy?ski M, K?obus G
Journal of experimental botany59(13)3721-8(2008)

Two plasma membrane H(+)-ATPase genes are differentially expressed in iron-deficient cucumber plants.
Santi S, Cesco S, Varanini Z, Pinton R
Plant physiology and biochemistry : PPB43(3)287-92(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu36444	NM_001305750.1 Latest version!	Cucumis sativus plasma membrane ATPase 4 (HA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HA3 ( NM_001305750.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001292679.1 Cucumis sativus plasma membrane ATPase 4 (HA3), mRNA.

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CloneID	OCu36444
Clone ID Related Accession (Same CDS sequence)	NM_001305750.1
Accession Version	NM_001305750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	plasma membrane ATPase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF375892.2. On Mar 30, 2015 this sequence version replaced XM_004146279.1. ##Evidence-Data-START## Transcript exon combination :: EF375892.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04372267, SAMN04457411 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGAA AATTGAGTCT GGAGGAGATC AAGAACGAAA CTGTTGACCT GGAGAAAATA 
CCAATTGCAG AAGTGTTTGA ACAATTGAAA TGTGGCCCAG AAGGTCTCAC AACTCAAGAA 
GGAGAAAACA GACTCCAATT ATTTGGTCCC AACAAATTAG AAGAGAAAAA GGAAAGCAAA 
ATACTAAAGT TTTTGGGGTT CATGTGGAAT CCTTTGTCAT GGGTTATGGA AGCTGCAGCT 
ATTATGGCTA TTGCTTTAGC AAATGGTGGT GGAAAGCCTC CAGATTGGGA AGACTTTGTT 
GGTATTATTT GTTTATTAGT GATCAACTCC ACAATCAGTT TCATTGAGGA AAATAATGCT 
GGCAATGCTG CTGCTGCTCT CATGGCTGGC CTTGCCCCTA AAACCAAGGT ACTGAGGGAT 
GGTAAATGGA GTGAGCAGGA GGCTGCAATT TTGGTTCCTG GAGATATCGT TAGTGTAAAA 
TTGGGAGATA TTATCCCGGC GGATGCTCGT CTTCTCGAGG GCGATCCTTT AAAGGTTGAC 
CAATCTGCAT TGACTGGAGA GTCACTTCCT GTTACTAAAA ATCCAGGGGA TGAAATCTTC 
TCTGGTTCAA CTTGCAAACA AGGGGAAATT GAAGCTGTTG TAATTGCCAC TGGTGTGCAT 
ACTTTCTTTG GAAAAGCAGC ACATCTTGTA GATAGCACTC ACCAAGTTGG GCATTTCCAG 
AAAGTGCTTA CAGCAATTGG GAATTTTTGT ATTTGTTCTA TTGCTTTGGG AATGCTTATT 
GAAATCATTG GCATGTGCCC AATTCAACAT CGTAAATACC GTGACGGAAT CGACAATCTC 
TTCGTTCTCT TGATCGGTGG CATACCAATT GCTATGCCTA CTGTATTATC TGTGACAATG 
GCTATTGGAT CTCACAAACT ATCTCAACAA GGCGCCATCA CGAAGCGTAT GACTGCCATT 
GAAGAAATGG CTGGTATGGA TGTCCTTTGC AGTGATAAAA CAGGAACATT GACTCTAAAC 
AAGCTTAGTG TTGATGTAAA TTTGGTCGAG GTTTTTGCTA AGGGTGTGGA TAAACAACAT 
GTTATTTTGC TGGCTGCAAG GGCTTCTCGA ACCGAAAATC AAGATGCAAT TGATGCTGCT 
ATTGTAGGAA TGCTTGCTGA TCCTAAGGAG GCACGAGCAG GCATAAGAGA AGTGCATTTC 
TTCCCATTCA ATCCTGTGGA TAAGAGAACT GCATTAACTT ACATTGATTC TGATGGTAAT 
TGGCATCGAG CAAGCAAAGG AGCTCCCGAG CAGATCTTAA CACTTTGCAA CTGCAAAGAG 
GATGTCAAGA AGAAGGCTCA TGCTGTAATT GATAAATTTG CAGAACGCGG TCTTCGCTCG 
CTGGCTGTTG GGAGACAGGA AGTGCCTGAG AAAAGAAAAG AAAGTCCTGG AAGTCCATGG 
CAGTTTGTTG GCTTATTGCC TCTGTTTGAT CCTCCAAGGC ATGACAGTGG AGAAACCATC 
AAAAGAGCTC TCAATCTGGG AGTGAATGTC AAGATGATTA CCGGTGATCA ACTTGCTATT 
GCCAAAGAAA CTGGTAGAAG ACTTGGAATG GGGACAAACA TGTATCCGTC TGCATCATTA 
CTTGGCCAAC ACAAGGATGA AAGCATAGCA GGCATTCCTA TTGAAGAGTT AATTGAAAAG 
GCAGATGGCT TTGCTGGAGT TTTTCCAGAA CACAAATACG AGATTGTAAG GAAGTTGCAA 
GAGAGGAAAC ATATTTGTGG AATGACCGGA GACGGTGTGA ATGATGCTCC TGCACTAAAA 
AAGGCTGACA TTGGAATTGC AGTTGCTGAT GCTACTGATG CTGCTAGAGG TGCTTCTGAT 
ATTGTTCTCA CAGAGCCAGG CTTGAGTGTT ATCATCAGTG CTGTCCTTAC GAGTAGAGCT 
ATCTTTCAGA GAATGAAGAA CTACACAATT TATGCAGTTT CGATCACAAT ACGTATCGTG 
TTTGGTTTCT TGCTTATTGC CCTCATATGG AAATTTGACT TCTCCCCATT TATGGTTTTG 
ATCATTGCCA TTCTAAATGA TGGTACAATC ATGACCATTT CAAAGGATAG AGTGAAGCCA 
TCTCCATTAC CTGATAGTTG GAAACTGAAA GAGATATTTG CAACAGGCAT CATGCTTGGT 
GGTTACTTGG CATTGATGAC TGTCATATTC TTCTGGATAA TGAGGGAGAC AGATTTCTTC 
CCAGAGAAAT TTAATGTGAG ACCTATTAAG GACAGTCCTA AAGAAATGAT GGCTGCTCTT 
TATCTTCAAG TGAGCATTGT CAGCCAAGCT TTGATATTTG TTACTCGATC GCGTAGTTGG 
TCCTACTTTG AACGACCGGG GTTGCTACTG ATGGGTGCTT TTGTTATTGC ACAACTGGTT 
GCAACACTCA TAGCAGTATA TGCAAACTGG GAATTTGCAA AGATAAAAGG AGCAGGATGG 
GGATGGGCTG GTGTAGTTTG GATCTACAGT GTTATTTTCT ACATTCCATT AGACTTTATC 
AAGTTTGCAA TTCGCTATAT CTTAAGCGGC AAAGCTTGGC TTAACTTACT GGAAAACAAG 
ACCGCCTTTA CGACCAAGAA AGACTATGGA AGGGAGGAAA GAGAAGCTCA ATGGGCTGCT 
ACTCAAAGAA CCTTACATGG CCTCCAACCA GCACCAGAAA GAGCTAGTCT CTTCCTTGAA 
AAGAATAGCT ACAGAGAGCT TTCCGAAATC GCGGAGCAGG CCAAGAGACG AGCAGAGATA 
GCTAGGCTTA GGGAGCTCAA CACTTTGAAG GGTCATGTTG AATCAGTAGT AAAATTGAAA 
GGGTTGGATA TTGATACAAT CCAACAACAT TACACAGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292679.1
CDS	157..3018
Translation	MAGKLSLEEIKNETVDLEKIPIAEVFEQLKCGPEGLTTQEGENRLQLFGPNKLEEKKESKILKFLGFMWNPLSWVMEAAAIMAIALANGGGKPPDWEDFVGIICLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGKWSEQEAAILVPGDIVSVKLGDIIPADARLLEGDPLKVDQSALTGESLPVTKNPGDEIFSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTHQVGHFQKVLTAIGNFCICSIALGMLIEIIGMCPIQHRKYRDGIDNLFVLLIGGIPIAMPTVLSVTMAIGSHKLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDVNLVEVFAKGVDKQHVILLAARASRTENQDAIDAAIVGMLADPKEARAGIREVHFFPFNPVDKRTALTYIDSDGNWHRASKGAPEQILTLCNCKEDVKKKAHAVIDKFAERGLRSLAVGRQEVPEKRKESPGSPWQFVGLLPLFDPPRHDSGETIKRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSASLLGQHKDESIAGIPIEELIEKADGFAGVFPEHKYEIVRKLQERKHICGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFLLIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLKEIFATGIMLGGYLALMTVIFFWIMRETDFFPEKFNVRPIKDSPKEMMAALYLQVSIVSQALIFVTRSRSWSYFERPGLLLMGAFVIAQLVATLIAVYANWEFAKIKGAGWGWAGVVWIYSVIFYIPLDFIKFAIRYILSGKAWLNLLENKTAFTTKKDYGREEREAQWAATQRTLHGLQPAPERASLFLEKNSYRELSEIAEQAKRRAEIARLRELNTLKGHVESVVKLKGLDIDTIQQHYTV

Target ORF information:

RefSeq Version	NM_001305750.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus plasma membrane ATPase 4 (HA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305750.1

ORF Insert Sequence:

ATGGCTGGAA AATTGAGTCT GGAGGAGATC AAGAACGAAA CTGTTGACCT GGAGAAAATA 
CCAATTGCAG AAGTGTTTGA ACAATTGAAA TGTGGCCCAG AAGGTCTCAC AACTCAAGAA 
GGAGAAAACA GACTCCAATT ATTTGGTCCC AACAAATTAG AAGAGAAAAA GGAAAGCAAA 
ATACTAAAGT TTTTGGGGTT CATGTGGAAT CCTTTGTCAT GGGTTATGGA AGCTGCAGCT 
ATTATGGCTA TTGCTTTAGC AAATGGTGGT GGAAAGCCTC CAGATTGGGA AGACTTTGTT 
GGTATTATTT GTTTATTAGT GATCAACTCC ACAATCAGTT TCATTGAGGA AAATAATGCT 
GGCAATGCTG CTGCTGCTCT CATGGCTGGC CTTGCCCCTA AAACCAAGGT ACTGAGGGAT 
GGTAAATGGA GTGAGCAGGA GGCTGCAATT TTGGTTCCTG GAGATATCGT TAGTGTAAAA 
TTGGGAGATA TTATCCCGGC GGATGCTCGT CTTCTCGAGG GCGATCCTTT AAAGGTTGAC 
CAATCTGCAT TGACTGGAGA GTCACTTCCT GTTACTAAAA ATCCAGGGGA TGAAATCTTC 
TCTGGTTCAA CTTGCAAACA AGGGGAAATT GAAGCTGTTG TAATTGCCAC TGGTGTGCAT 
ACTTTCTTTG GAAAAGCAGC ACATCTTGTA GATAGCACTC ACCAAGTTGG GCATTTCCAG 
AAAGTGCTTA CAGCAATTGG GAATTTTTGT ATTTGTTCTA TTGCTTTGGG AATGCTTATT 
GAAATCATTG GCATGTGCCC AATTCAACAT CGTAAATACC GTGACGGAAT CGACAATCTC 
TTCGTTCTCT TGATCGGTGG CATACCAATT GCTATGCCTA CTGTATTATC TGTGACAATG 
GCTATTGGAT CTCACAAACT ATCTCAACAA GGCGCCATCA CGAAGCGTAT GACTGCCATT 
GAAGAAATGG CTGGTATGGA TGTCCTTTGC AGTGATAAAA CAGGAACATT GACTCTAAAC 
AAGCTTAGTG TTGATGTAAA TTTGGTCGAG GTTTTTGCTA AGGGTGTGGA TAAACAACAT 
GTTATTTTGC TGGCTGCAAG GGCTTCTCGA ACCGAAAATC AAGATGCAAT TGATGCTGCT 
ATTGTAGGAA TGCTTGCTGA TCCTAAGGAG GCACGAGCAG GCATAAGAGA AGTGCATTTC 
TTCCCATTCA ATCCTGTGGA TAAGAGAACT GCATTAACTT ACATTGATTC TGATGGTAAT 
TGGCATCGAG CAAGCAAAGG AGCTCCCGAG CAGATCTTAA CACTTTGCAA CTGCAAAGAG 
GATGTCAAGA AGAAGGCTCA TGCTGTAATT GATAAATTTG CAGAACGCGG TCTTCGCTCG 
CTGGCTGTTG GGAGACAGGA AGTGCCTGAG AAAAGAAAAG AAAGTCCTGG AAGTCCATGG 
CAGTTTGTTG GCTTATTGCC TCTGTTTGAT CCTCCAAGGC ATGACAGTGG AGAAACCATC 
AAAAGAGCTC TCAATCTGGG AGTGAATGTC AAGATGATTA CCGGTGATCA ACTTGCTATT 
GCCAAAGAAA CTGGTAGAAG ACTTGGAATG GGGACAAACA TGTATCCGTC TGCATCATTA 
CTTGGCCAAC ACAAGGATGA AAGCATAGCA GGCATTCCTA TTGAAGAGTT AATTGAAAAG 
GCAGATGGCT TTGCTGGAGT TTTTCCAGAA CACAAATACG AGATTGTAAG GAAGTTGCAA 
GAGAGGAAAC ATATTTGTGG AATGACCGGA GACGGTGTGA ATGATGCTCC TGCACTAAAA 
AAGGCTGACA TTGGAATTGC AGTTGCTGAT GCTACTGATG CTGCTAGAGG TGCTTCTGAT 
ATTGTTCTCA CAGAGCCAGG CTTGAGTGTT ATCATCAGTG CTGTCCTTAC GAGTAGAGCT 
ATCTTTCAGA GAATGAAGAA CTACACAATT TATGCAGTTT CGATCACAAT ACGTATCGTG 
TTTGGTTTCT TGCTTATTGC CCTCATATGG AAATTTGACT TCTCCCCATT TATGGTTTTG 
ATCATTGCCA TTCTAAATGA TGGTACAATC ATGACCATTT CAAAGGATAG AGTGAAGCCA 
TCTCCATTAC CTGATAGTTG GAAACTGAAA GAGATATTTG CAACAGGCAT CATGCTTGGT 
GGTTACTTGG CATTGATGAC TGTCATATTC TTCTGGATAA TGAGGGAGAC AGATTTCTTC 
CCAGAGAAAT TTAATGTGAG ACCTATTAAG GACAGTCCTA AAGAAATGAT GGCTGCTCTT 
TATCTTCAAG TGAGCATTGT CAGCCAAGCT TTGATATTTG TTACTCGATC GCGTAGTTGG 
TCCTACTTTG AACGACCGGG GTTGCTACTG ATGGGTGCTT TTGTTATTGC ACAACTGGTT 
GCAACACTCA TAGCAGTATA TGCAAACTGG GAATTTGCAA AGATAAAAGG AGCAGGATGG 
GGATGGGCTG GTGTAGTTTG GATCTACAGT GTTATTTTCT ACATTCCATT AGACTTTATC 
AAGTTTGCAA TTCGCTATAT CTTAAGCGGC AAAGCTTGGC TTAACTTACT GGAAAACAAG 
ACCGCCTTTA CGACCAAGAA AGACTATGGA AGGGAGGAAA GAGAAGCTCA ATGGGCTGCT 
ACTCAAAGAA CCTTACATGG CCTCCAACCA GCACCAGAAA GAGCTAGTCT CTTCCTTGAA 
AAGAATAGCT ACAGAGAGCT TTCCGAAATC GCGGAGCAGG CCAAGAGACG AGCAGAGATA 
GCTAGGCTTA GGGAGCTCAA CACTTTGAAG GGTCATGTTG AATCAGTAGT AAAATTGAAA 
GGGTTGGATA TTGATACAAT CCAACAACAT TACACAGTGT AA