LOC101221653 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101221653
Entrez Gene ID	101221653
Full Name	1-aminocyclopropane-1-carboxylate oxidase 4
Synonyms	CS-ACO2,Cs-ACO1,aco1
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308849.1	NP_001295778.1	1-aminocyclopropane-1-carboxylate oxidase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
Li Z, Zhang Z, Yan P, Huang S, Fei Z, Lin K
BMC genomics12540(2011 Nov)

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
Guo S, Zheng Y, Joung JG, Liu S, Zhang Z, Crasta OR, Sobral BW, Xu Y, Huang S, Fei Z
BMC genomics11384(2010 Jun)

The genome of the cucumber, Cucumis sativus L.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EA, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, Du Y, Li S
Nature genetics41(12)1275-81(2009 Dec)

An integrated genetic and cytogenetic map of the cucumber genome.
Ren Y, Zhang Z, Liu J, Staub JE, Han Y, Cheng Z, Li X, Lu J, Miao H, Kang H, Xie B, Gu X, Wang X, Du Y, Jin W, Huang S
PloS one4(6)e5795(2009 Jun)

Expression of ACC oxidase genes differs among sex genotypes and sex phases in cucumber.
Kahana A, Silberstein L, Kessler N, Goldstein RS, Perl-Treves R
Plant molecular biology41(4)517-28(1999 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101221653 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101221653 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu03750	NM_001308849.1 Latest version!	Cucumis sativus 1-aminocyclopropane-1-carboxylate oxidase 4 (LOC101221653), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101221653 ( NM_001308849.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001295778.1 Cucumis sativus 1-aminocyclopropane-1-carboxylate oxidase 4 (LOC101221653), mRNA.

Price & Availability↑

CloneID	OCu03750
Clone ID Related Accession (Same CDS sequence)	NM_001308849.1
Accession Version	NM_001308849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-26
Organism	Cucumis sativus(cucumber)
Product	1-aminocyclopropane-1-carboxylate oxidase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FN544066.1. On May 16, 2015 this sequence version replaced XM_004151234.2. ##Evidence-Data-START## Transcript exon combination :: FN544066.1, AB006807.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00007319, SAMN00007327 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTTCC CAATCATCAA CTTGGAGAAG CTTAATGGTG AAGAGAGATC AAGTATCTTA 
GCCAAAATCA AAGATGCTTG TGAAAACTGG GGTTTCTTTG AGGTACTGAA CCACGGAATA 
GATACAGAAT TTCTAGACAA AGTAGAGAAA TTAACAAAAG AACATTACAA GAAATGTATG 
GAAGAGAGAT TCAAAGAACA CATAGCCAGC AAAGGATTGA ACGACGTCGT AACAGAAGTC 
AACGATGTTG ACTGGGAAAG CACTTTCTTC CTCCGTCATC TCCCAGTCTC CAACATCGCG 
GATCTCCCGG AACTATCCGA CGATTACAAA TCCGTAATGA AACAATTCGC GTCAAAATTA 
CAAGTACTCG CCGAACTCCT CCTGGATTTA CTCTGCGAAA ATCTGAACCT TCCAAAATCA 
TATCTGAAAA ACGCTTTCCA CGGATCCAAC GGCCCAACTT TCGGGACAAA AGTCAGCAAT 
TACCCACCTT GTCCAAACCC AGATCTAATA AAAGGCCTCC GAGCACACAC CGACGCGGGA 
GGGATAATCC TTCTATTTCA AGACGACAAA GTGAGTGGTT TGCAACTGTT GAAGGAGGGG 
GAGTGGGTGG ACGTGCCGCC GGTCCGGCTC TCGATCGTGG TGAATATCGG GGATCAATTG 
GAAGTGATTA CAAACGGGAA ATATAAAAGT GTTTTGCACA GAGTAATTGC GCAGCCGGAA 
GGGGAAGGGA GAATGTCGTT GGCGTCGTTT TACAATCCGG GAAGTGACGC GGTGATATTT 
CCGGCGCCGA GTTTGGTGGC GGAGGAGAAA AATGAAACAT ACCCAAAATT TGTATTTGAG 
GATTATATGA AGCTTTATGC TGGGCTGAAA TTTGAAGCCA AGGAGCCGAG GTTTGAAGCC 
ATGAAGGGTA ATAATGTCAA ATTGGATCCT ATTGCAACCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295778.1
CDS	1..945
Translation	MEFPIINLEKLNGEERSSILAKIKDACENWGFFEVLNHGIDTEFLDKVEKLTKEHYKKCMEERFKEHIASKGLNDVVTEVNDVDWESTFFLRHLPVSNIADLPELSDDYKSVMKQFASKLQVLAELLLDLLCENLNLPKSYLKNAFHGSNGPTFGTKVSNYPPCPNPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKEGEWVDVPPVRLSIVVNIGDQLEVITNGKYKSVLHRVIAQPEGEGRMSLASFYNPGSDAVIFPAPSLVAEEKNETYPKFVFEDYMKLYAGLKFEAKEPRFEAMKGNNVKLDPIATV

Target ORF information:

RefSeq Version	NM_001308849.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus 1-aminocyclopropane-1-carboxylate oxidase 4 (LOC101221653), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308849.1

ORF Insert Sequence:

ATGGAGTTCC CAATCATCAA CTTGGAGAAG CTTAATGGTG AAGAGAGATC AAGTATCTTA 
GCCAAAATCA AAGATGCTTG TGAAAACTGG GGTTTCTTTG AGGTACTGAA CCACGGAATA 
GATACAGAAT TTCTAGACAA AGTAGAGAAA TTAACAAAAG AACATTACAA GAAATGTATG 
GAAGAGAGAT TCAAAGAACA CATAGCCAGC AAAGGATTGA ACGACGTCGT AACAGAAGTC 
AACGATGTTG ACTGGGAAAG CACTTTCTTC CTCCGTCATC TCCCAGTCTC CAACATCGCG 
GATCTCCCGG AACTATCCGA CGATTACAAA TCCGTAATGA AACAATTCGC GTCAAAATTA 
CAAGTACTCG CCGAACTCCT CCTGGATTTA CTCTGCGAAA ATCTGAACCT TCCAAAATCA 
TATCTGAAAA ACGCTTTCCA CGGATCCAAC GGCCCAACTT TCGGGACAAA AGTCAGCAAT 
TACCCACCTT GTCCAAACCC AGATCTAATA AAAGGCCTCC GAGCACACAC CGACGCGGGA 
GGGATAATCC TTCTATTTCA AGACGACAAA GTGAGTGGTT TGCAACTGTT GAAGGAGGGG 
GAGTGGGTGG ACGTGCCGCC GGTCCGGCTC TCGATCGTGG TGAATATCGG GGATCAATTG 
GAAGTGATTA CAAACGGGAA ATATAAAAGT GTTTTGCACA GAGTAATTGC GCAGCCGGAA 
GGGGAAGGGA GAATGTCGTT GGCGTCGTTT TACAATCCGG GAAGTGACGC GGTGATATTT 
CCGGCGCCGA GTTTGGTGGC GGAGGAGAAA AATGAAACAT ACCCAAAATT TGTATTTGAG 
GATTATATGA AGCTTTATGC TGGGCTGAAA TTTGAAGCCA AGGAGCCGAG GTTTGAAGCC 
ATGAAGGGTA ATAATGTCAA ATTGGATCCT ATTGCAACCG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
BMC genomics12540(2011 Nov)
Li Z,Zhang Z,Yan P,Huang S,Fei Z,Lin K

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
BMC genomics11384(2010 Jun)
Guo S,Zheng Y,Joung JG,Liu S,Zhang Z,Crasta OR,Sobral BW,Xu Y,Huang S,Fei Z

The genome of the cucumber, Cucumis sativus L.
Nature genetics41(12)1275-81(2009 Dec)
Huang S,Li R,Zhang Z,Li L,Gu X,Fan W,Lucas WJ,Wang X,Xie B,Ni P,Ren Y,Zhu H,Li J,Lin K,Jin W,Fei Z,Li G,Staub J,Kilian A,van der Vossen EA,Wu Y,Guo J,He J,Jia Z,Ren Y,Tian G,Lu Y,Ruan J,Qian W,Wang M,Huang Q,Li B,Xuan Z,Cao J,Asan,Wu Z,Zhang J,Cai Q,Bai Y,Zhao B,Han Y,Li Y,Li X,Wang S,Shi Q,Liu S,Cho WK,Kim JY,Xu Y,Heller-Uszynska K,Miao H,Cheng Z,Zhang S,Wu J,Yang Y,Kang H,Li M,Liang H,Ren X,Shi Z,Wen M,Jian M,Yang H,Zhang G,Yang Z,Chen R,Liu S,Li J,Ma L,Liu H,Zhou Y,Zhao J,Fang X,Li G,Fang L,Li Y,Liu D,Zheng H,Zhang Y,Qin N,Li Z,Yang G,Yang S,Bolund L,Kristiansen K,Zheng H,Li S,Zhang X,Yang H,Wang J,Sun R,Zhang B,Jiang S,Wang J,Du Y,Li S

An integrated genetic and cytogenetic map of the cucumber genome.
PloS one4(6)e5795(2009 Jun)
Ren Y,Zhang Z,Liu J,Staub JE,Han Y,Cheng Z,Li X,Lu J,Miao H,Kang H,Xie B,Gu X,Wang X,Du Y,Jin W,Huang S

Expression of ACC oxidase genes differs among sex genotypes and sex phases in cucumber.
Plant molecular biology41(4)517-28(1999 Nov)
Kahana A,Silberstein L,Kessler N,Goldstein RS,Perl-Treves R

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LOC101221653 cDNA ORF clone, Cucumis sativus(cucumber)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム