HA2 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	HA2
Entrez Gene ID	101221564
Full Name	plasma membrane ATPase 4
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305767.1	NP_001292696.1	plasma membrane ATPase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Two plasma membrane H(+)-ATPase genes are differentially expressed in iron-deficient cucumber plants.
Santi S, Cesco S, Varanini Z, Pinton R
Plant physiology and biochemistry : PPB43(3)287-92(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu03741	NM_001305767.1 Latest version!	Cucumis sativus plasma membrane ATPase 4 (HA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HA2 ( NM_001305767.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001292696.1 Cucumis sativus plasma membrane ATPase 4 (HA2), mRNA.

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CloneID	OCu03741
Clone ID Related Accession (Same CDS sequence)	NM_001305767.1
Accession Version	NM_001305767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	plasma membrane ATPase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU735752.2. On Mar 30, 2015 this sequence version replaced XM_004148278.2. ##Evidence-Data-START## Transcript exon combination :: EU735752.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01085196, SAMN01085198 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAACG ACACCGCTTT AACTCTCGAA GAGATCAAGA ATGAAACTGT TGATTTGGAG 
AAAATCCCCA TTGAGGAAGT GTTTGAGCAG CTGAAATGTA CAAGAGAAGG TTTAAGTTCT 
CAAGAAGGGG AGAATCGTCT CCAAATCTTT GGTCCCAACA AGCTAGAAGA GAAAAAGGAA 
AGCAAGATCT TGAAGTTTCT TGGGTTTATG TGGAATCCTC TGTCTTGGGT CATGGAAGCT 
GCAGCTATCA TGGCCATTGC CTTGGCCAAC GGTGGCAATC GGGGACCGGA TTGGCAGGAT 
TTCGTTGGTA TCGTCTGTCT TTTGGTTATC AACTCCACCA TTAGTTTCAT TGAAGAGAAC 
AATGCTGGTA ATGCAGCTGC TGCTCTTATG GCTGGTCTTG CACCTAAAAC CAAGGTTCTG 
AGGGATGGAA AATGGTGTGA ACAAGATGCA TCAATTCTAG TACCAGGAGA TGTGATCAGT 
GTGAAATTGG GAGATATCAT CCCAGCCGAT GCTCGTCTTC TCGAAGGTGA TCCTTTAAAG 
GTTGATCAAT CTGCCTTGAC CGGAGAATCA CTTCCCGTGA CCAAGAATCC AGGGGATGAA 
GTTTTCTCTG GTTCCACTTG CAAACAAGGA GAAATCGAGG CTATTGTTAT TGCTACTGGT 
GTGCACACTT TCTTTGGTAA AGCAGCTCAT CTTGTTGATA GTACTAACCA AGTTGGACAC 
TTCCAAAAGG TTCTCACTGC CATTGGAAAC TTTTGTATTT GCTCCATTGC CATTGGTATG 
TTGATTGAGA TCCTCGTTAT GTACCCGATC CAACACCGTG CCTATAGAGA TGGTATCGAC 
AACCTTTTGG TTCTCTTGAT TGGAGGCATT CCAATTGCTA TGCCTACTGT CTTGTCAGTG 
ACTATGGCTA TTGGTTCTCA CAAGCTGTCT CAACAAGGTG CTATTACCAA GAGAATGACT 
GCTATCGAAG AAATGGCCGG TATGGACGTA CTTTGCAGTG ACAAAACTGG AACTCTGACT 
CTTAACAAGT TGAGTGTTGA CAAGAACTTG ATTGAGGTGT TTGTCAAGGG TGTTGACAAA 
GAACATGTCA TTCTGCTTGC TGCAAGGGCA TCTAGGACTG AAAACCAGGA TGCAATTGAT 
GCTGCCATGG TTGGAATGCT CGCTGACCCG AAAGAGGCAA GAGCTGGAAT TAGAGAAATT 
CACTTCTTCC CATTTAATCC TGTGGATAAG AGAACTGCTT TGACTTACAT TGATTCTAAT 
GGAAACTGGC ACCGTGCTAG CAAAGGTGCT CCTGAACAGA TCTTGACTCT TTGCAACTGC 
AAAGAGGACT TCAAGAGGAA GGTGTTTTCT GTAATTGACA AGTTTGCCGA GCGTGGACTT 
CGATCTTTGG CTGTTTCTAG ACAGGAAGTG CCTGAAAAGA ATAAGGAAAG TCCCGGTGCT 
CCATGGCAAT TTGTTGGTTT GTTGCCCCTT TTCGATCCTC CTAGACACGA TAGTGCTGAA 
ACCATCCGCA GGGCTCTCAA CTTGGGTGTG AATGTCAAGA TGATTACTGG TGATCAACTT 
GCTATTGCAA AGGAAACCGG TCGAAGACTT GGAATGGGAA CGAACATGTA TCCATCTTCT 
TCCTTACTCG GTCAGCACAA GGACGAAAGC ATTGCTGGCC TTCCAGTTGA GGAGTTGATT 
GAGAAGGCTG ATGGATTTGC TGGAGTGTTT CCAGAACACA AATACGAAAT CGTAAAGAAG 
TTGCAAGAAA GAAAGCACAT TTGTGGAATG ACTGGAGATG GTGTGAATGA TGCTCCTGCT 
TTGAAGAAGG CAGACATTGG TATTGCTGTT GCTGATGCTA CTGATGCTGC ACGAGGTGCA 
TCTGACATTG TTCTTACCGA GCCTGGTTTG AGCGTTATCA TTAGTGCCGT GTTGACCAGC 
AGAGCAATTT TCCAAAGGAT GAAGAACTAT ACTATTTATG CAGTCTCCAT CACTATTCGT 
ATTGTGTTTG GTTTCATGTT GATTGCTCTC ATTTGGAAAT TTGATTTCTC TCCCTTCATG 
GTTTTGATTA TTGCTATCTT GAACGATGGT ACAATTATGA CCATCTCAAA GGACAGGGTG 
AAGCCATCTC CATTACCTGA CAGTTGGAAA CTAAAGGAGA TTTTCGCAAC CGGAATAGTA 
CTTGGTGGGT ACTTGGCCTT GATGACGGTT ATATTCTTTT GGTTAATGGA CGGAACAAAT 
TTCTTTTCGG ATAAATTCGG TGTTAGATCC ATCCGTCATA ATGAAGATGA GATGATGGCT 
GCTTTGTACC TGCAAGTCAG TATTGTCAGC CAGGCTTTGA TTTTTGTAAC AAGGTCGCGT 
GGCCGGTCGT ACGCTGAGCG ACCTGGACTT CTATTGGTCG GCGCTTTCTT CATTGCACAG 
CTGGTTGCAA CCTTGATCGC AGTATATGCG AACTGGGGAT TTGCAAAGAT CAAGGGAATC 
GGATGGGGAT GGGCCGGTGT CATCTGGCTT TACAGCATTG TTTTCTATAT TCCACTCGAT 
GTCATGAAGT TCGCTATTCG ATACATATTG AGCGGAAAGG CATGGCTCAA CTTGTTAGAA 
AACAAGACCG CTTTCACCAC CAAGAAAGAC TATGGAAAAG AGGAAAGAGA AGCACAATGG 
GCACTTGCTC AAAGAACATT ACATGGACTT CAACCACCCG AGTCGACAAA CATCTTCTCC 
GAAAAGAGCA GCTACAGAGA ACTCTCTGAG ATCGCCGAGC AAGCTAAGAG ACGTGCCGAG 
ATTGCAAGGC TCCGAGAGCT GAACACACTC AAGGGACACG TCGAGTCTGT GGTGAAGTTG 
AAGGGACTCG ACATCGACAC CATTCAGCAG CACTACACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292696.1
CDS	231..3095
Translation	MGNDTALTLEEIKNETVDLEKIPIEEVFEQLKCTREGLSSQEGENRLQIFGPNKLEEKKESKILKFLGFMWNPLSWVMEAAAIMAIALANGGNRGPDWQDFVGIVCLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGKWCEQDASILVPGDVISVKLGDIIPADARLLEGDPLKVDQSALTGESLPVTKNPGDEVFSGSTCKQGEIEAIVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCICSIAIGMLIEILVMYPIQHRAYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHKLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDKNLIEVFVKGVDKEHVILLAARASRTENQDAIDAAMVGMLADPKEARAGIREIHFFPFNPVDKRTALTYIDSNGNWHRASKGAPEQILTLCNCKEDFKRKVFSVIDKFAERGLRSLAVSRQEVPEKNKESPGAPWQFVGLLPLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSSSLLGQHKDESIAGLPVEELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMLIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLKEIFATGIVLGGYLALMTVIFFWLMDGTNFFSDKFGVRSIRHNEDEMMAALYLQVSIVSQALIFVTRSRGRSYAERPGLLLVGAFFIAQLVATLIAVYANWGFAKIKGIGWGWAGVIWLYSIVFYIPLDVMKFAIRYILSGKAWLNLLENKTAFTTKKDYGKEEREAQWALAQRTLHGLQPPESTNIFSEKSSYRELSEIAEQAKRRAEIARLRELNTLKGHVESVVKLKGLDIDTIQQHYTV

Target ORF information:

RefSeq Version	NM_001305767.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus plasma membrane ATPase 4 (HA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305767.1

ORF Insert Sequence:

ATGGGAAACG ACACCGCTTT AACTCTCGAA GAGATCAAGA ATGAAACTGT TGATTTGGAG 
AAAATCCCCA TTGAGGAAGT GTTTGAGCAG CTGAAATGTA CAAGAGAAGG TTTAAGTTCT 
CAAGAAGGGG AGAATCGTCT CCAAATCTTT GGTCCCAACA AGCTAGAAGA GAAAAAGGAA 
AGCAAGATCT TGAAGTTTCT TGGGTTTATG TGGAATCCTC TGTCTTGGGT CATGGAAGCT 
GCAGCTATCA TGGCCATTGC CTTGGCCAAC GGTGGCAATC GGGGACCGGA TTGGCAGGAT 
TTCGTTGGTA TCGTCTGTCT TTTGGTTATC AACTCCACCA TTAGTTTCAT TGAAGAGAAC 
AATGCTGGTA ATGCAGCTGC TGCTCTTATG GCTGGTCTTG CACCTAAAAC CAAGGTTCTG 
AGGGATGGAA AATGGTGTGA ACAAGATGCA TCAATTCTAG TACCAGGAGA TGTGATCAGT 
GTGAAATTGG GAGATATCAT CCCAGCCGAT GCTCGTCTTC TCGAAGGTGA TCCTTTAAAG 
GTTGATCAAT CTGCCTTGAC CGGAGAATCA CTTCCCGTGA CCAAGAATCC AGGGGATGAA 
GTTTTCTCTG GTTCCACTTG CAAACAAGGA GAAATCGAGG CTATTGTTAT TGCTACTGGT 
GTGCACACTT TCTTTGGTAA AGCAGCTCAT CTTGTTGATA GTACTAACCA AGTTGGACAC 
TTCCAAAAGG TTCTCACTGC CATTGGAAAC TTTTGTATTT GCTCCATTGC CATTGGTATG 
TTGATTGAGA TCCTCGTTAT GTACCCGATC CAACACCGTG CCTATAGAGA TGGTATCGAC 
AACCTTTTGG TTCTCTTGAT TGGAGGCATT CCAATTGCTA TGCCTACTGT CTTGTCAGTG 
ACTATGGCTA TTGGTTCTCA CAAGCTGTCT CAACAAGGTG CTATTACCAA GAGAATGACT 
GCTATCGAAG AAATGGCCGG TATGGACGTA CTTTGCAGTG ACAAAACTGG AACTCTGACT 
CTTAACAAGT TGAGTGTTGA CAAGAACTTG ATTGAGGTGT TTGTCAAGGG TGTTGACAAA 
GAACATGTCA TTCTGCTTGC TGCAAGGGCA TCTAGGACTG AAAACCAGGA TGCAATTGAT 
GCTGCCATGG TTGGAATGCT CGCTGACCCG AAAGAGGCAA GAGCTGGAAT TAGAGAAATT 
CACTTCTTCC CATTTAATCC TGTGGATAAG AGAACTGCTT TGACTTACAT TGATTCTAAT 
GGAAACTGGC ACCGTGCTAG CAAAGGTGCT CCTGAACAGA TCTTGACTCT TTGCAACTGC 
AAAGAGGACT TCAAGAGGAA GGTGTTTTCT GTAATTGACA AGTTTGCCGA GCGTGGACTT 
CGATCTTTGG CTGTTTCTAG ACAGGAAGTG CCTGAAAAGA ATAAGGAAAG TCCCGGTGCT 
CCATGGCAAT TTGTTGGTTT GTTGCCCCTT TTCGATCCTC CTAGACACGA TAGTGCTGAA 
ACCATCCGCA GGGCTCTCAA CTTGGGTGTG AATGTCAAGA TGATTACTGG TGATCAACTT 
GCTATTGCAA AGGAAACCGG TCGAAGACTT GGAATGGGAA CGAACATGTA TCCATCTTCT 
TCCTTACTCG GTCAGCACAA GGACGAAAGC ATTGCTGGCC TTCCAGTTGA GGAGTTGATT 
GAGAAGGCTG ATGGATTTGC TGGAGTGTTT CCAGAACACA AATACGAAAT CGTAAAGAAG 
TTGCAAGAAA GAAAGCACAT TTGTGGAATG ACTGGAGATG GTGTGAATGA TGCTCCTGCT 
TTGAAGAAGG CAGACATTGG TATTGCTGTT GCTGATGCTA CTGATGCTGC ACGAGGTGCA 
TCTGACATTG TTCTTACCGA GCCTGGTTTG AGCGTTATCA TTAGTGCCGT GTTGACCAGC 
AGAGCAATTT TCCAAAGGAT GAAGAACTAT ACTATTTATG CAGTCTCCAT CACTATTCGT 
ATTGTGTTTG GTTTCATGTT GATTGCTCTC ATTTGGAAAT TTGATTTCTC TCCCTTCATG 
GTTTTGATTA TTGCTATCTT GAACGATGGT ACAATTATGA CCATCTCAAA GGACAGGGTG 
AAGCCATCTC CATTACCTGA CAGTTGGAAA CTAAAGGAGA TTTTCGCAAC CGGAATAGTA 
CTTGGTGGGT ACTTGGCCTT GATGACGGTT ATATTCTTTT GGTTAATGGA CGGAACAAAT 
TTCTTTTCGG ATAAATTCGG TGTTAGATCC ATCCGTCATA ATGAAGATGA GATGATGGCT 
GCTTTGTACC TGCAAGTCAG TATTGTCAGC CAGGCTTTGA TTTTTGTAAC AAGGTCGCGT 
GGCCGGTCGT ACGCTGAGCG ACCTGGACTT CTATTGGTCG GCGCTTTCTT CATTGCACAG 
CTGGTTGCAA CCTTGATCGC AGTATATGCG AACTGGGGAT TTGCAAAGAT CAAGGGAATC 
GGATGGGGAT GGGCCGGTGT CATCTGGCTT TACAGCATTG TTTTCTATAT TCCACTCGAT 
GTCATGAAGT TCGCTATTCG ATACATATTG AGCGGAAAGG CATGGCTCAA CTTGTTAGAA 
AACAAGACCG CTTTCACCAC CAAGAAAGAC TATGGAAAAG AGGAAAGAGA AGCACAATGG 
GCACTTGCTC AAAGAACATT ACATGGACTT CAACCACCCG AGTCGACAAA CATCTTCTCC 
GAAAAGAGCA GCTACAGAGA ACTCTCTGAG ATCGCCGAGC AAGCTAAGAG ACGTGCCGAG 
ATTGCAAGGC TCCGAGAGCT GAACACACTC AAGGGACACG TCGAGTCTGT GGTGAAGTTG 
AAGGGACTCG ACATCGACAC CATTCAGCAG CACTACACAG TGTGA