LOC101219577 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101219577
Entrez Gene ID	101219577
Full Name	DNA gyrase subunit B, chloroplastic/mitochondrial
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004144285.2	XP_004144333.2	DNA gyrase subunit B, chloroplastic/mitochondrial
XM_004144285.3	XP_004144333.2	DNA gyrase subunit B, chloroplastic/mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC101219577 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101219577 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu64601	XM_004144285.3 Latest version!	Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
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OCu64601	XM_004144285.2	Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101219577 ( XM_004144285.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004144333.2 Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.

Price & Availability↑

CloneID	OCu64601
Clone ID Related Accession (Same CDS sequence)	XM_004144285.2 , XM_004144285.3
Accession Version	XM_004144285.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	DNA gyrase subunit B, chloroplastic/mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953806.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004144285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTC GCCAGCTTCT TCTTAGAGCT CCTCCTTATC TTCCCCTTCG CTTCATGGCT 
TCCTCAACCT CCTTTTTCCC TTCCTCTATA CACCTTCGCT TTCTTTCTTC CTCCGCCTCT 
TTCGCTCCTC GCTTCAGTTT CCTTCAGCTC AGGGGGGCGA GATTTCGAAC GATGAAGATT 
TCCACTAGTA TTCGACTGGT ACCAAATGCA GAAGTTTCTT CTAAGGCTTT CATGTCATCA 
AGTGCTGCTA CCGAAACATC AACGAGAAGT GAGAGTTCGA AGGCTTATGG GTCTGAGCAA 
ATTCAGGTTC TGGAAGGCTT AGACCCTGTA AGAAAGAGGC CTGGAATGTA TATTGGGAGC 
ACAGGACCTC GTGGACTACA TCATTTGGTC TATGAAATAC TGGATAACGC TGTTGATGAG 
GCTCAAGCTG GTTTTGCCTC ACAGATAGAT GTTGTCTTGC ATGCAGATAA TTCTGTGAGC 
GTCGCTGACA ATGGACGTGG GATTCCTACT GATTTGCATC CTGTTACGAA GAAGTCTTCC 
TTGGAGACTG TGTTGACGGT ATTGCATGCA GGTGGAAAAT TTGGTGGTTC CAGCAGTGGC 
TACAGTGTCT CAGGCGGACT ACATGGCGTG GGTCTGTCTG TGGTTAATGC CTTGTCTGAG 
TCCTTGGAAG TTTCTATTTG GCGTGATGGG AATGAGTTCC ACCAAAAGTA TTCTCGCGGA 
AAGCCTGCTT CCATTCTAAC ATCCCATGTT CCTTCATCTG AATTGAAAAA TCACCAAGGA 
ACACACATCA GATTTTGGCC TGACAAAGAA GTCTTTACTA CTGGAATACA ATTTGATTAC 
AACACAATAT CTGGACGAAT TAGGGAGCTA GCTTTTCTAA ACCCCAAGCT GACTATAACC 
CTCACAAAAG AGGATAATGA TTTGGAGAAG AACCAGTACA ATGAATACTG CTATGCTGGT 
GGCTTGGTCG AATATGTTGC ATGGCTGAAC AGTGATAAGA ATCCAATTCA TGAAGTCTTT 
GGTTTCAGAA AAGAAACAGA TGGCGTAGCA ATTGATGTCG CGCTTCAGTG GTGTGCAGAT 
GCATATTCAG ACACAATTCT AGGATATGCT AATAGCATAC GGACTATTGA TGGTGGCACC 
CATATAGATG GGATGAAGGC TTCACTGACG AGAACACTCA ATAATCTTGG AAAGAAATCA 
AAAATTTTTA AGGATAAGGA TATCAGCTTA AGTGGTGAAC ATGTTAGGGA GGGATTAACC 
TGTGTTGTCT CAGTCAGGGT CCGAAATCCG GAGTTTGAAG GACAGACCAA GACCAGGTTG 
GGAAATCCTG AAGTGCGAAA AGTAGTTGAG CAATCTATAC AAGAGTACCT AACTGAATAT 
CTGGAGTTGC ACCCTGATGT TCTTGAATTA ATCCTTTCAA AATCTTTAAA TGCTCTCAAG 
GCAGCGTTAG CTGCTAAGAG GGCAAGGGAG CTTGTGAGAC AAAAAAGTGT TTTGCGTTCA 
TCATCCCTTC CCGGCAAGCT AGCTGACTGT TCATCAATCA AACCTGAGGA GTCAGAAATT 
TTCATTGTTG AAGGAGACTC TGCCGGTGGA AGTGCAAAAC AGGGTCGTGA TAGGCGCTTC 
CAGGCTATTC TACCTTTGAG AGGCAAAATC TTAAACATTG AGAGGAAAGA CGAGGCTGCA 
ATGTACAAAA ATGAAGAGAT TAATAATTTA ATTCGTGCTC TTGGACTTGG ATTCAAGGGG 
GAAGATTTTA AAAAGGACGC TCTTCGCTAT CATAAAATAA TCATATTAAC GGATGCTGAT 
GTTGATGGTG CTCACATCCG AACTCTGCTC TTAACGTTTT TCTTCCGATA TCAGAAAGCT 
TTGTTTGACG AAGGGTGCAT TTATGTTGGT GTTCCACCTC TTTACAAGGT TGAGAGGGGG 
AAGCAAGTAT ACTACTGCTA CGACGACAAG GAACTAAAGA AGGTTCAAAG TTCCTTCCCA 
TCAAATGCAT CATATAATAT TCAGAGGTTC AAAGGTTTGG GTGAGATGAT GCCCGCACAA 
CTATGGGAAA CGACGATGGA CCCAGAACGA AGGATGCTGA AGCAGTTGGC AGTTGAGGAT 
GCAGCAGAAG CAAATGTCGT ATTTTCATCC CTTATGGGTG TCCGGGTGGA TTTTCGAAAG 
GAACTTATTC AGAAATCAGC AAGTACGATG AATCTTGAGC ATCTAGATAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004144333.2
CDS	53..2266
Translation	MALRQLLLRAPPYLPLRFMASSTSFFPSSIHLRFLSSSASFAPRFSFLQLRGARFRTMKISTSIRLVPNAEVSSKAFMSSSAATETSTRSESSKAYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSVADNGRGIPTDLHPVTKKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGNEFHQKYSRGKPASILTSHVPSSELKNHQGTHIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPKLTITLTKEDNDLEKNQYNEYCYAGGLVEYVAWLNSDKNPIHEVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIFKDKDISLSGEHVREGLTCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLELILSKSLNALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKDALRYHKIIILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQVYYCYDDKELKKVQSSFPSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGVRVDFRKELIQKSASTMNLEHLDI

Target ORF information:

RefSeq Version	XM_004144285.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004144285.2

ORF Insert Sequence:

ATGGCGCTTC GCCAGCTTCT TCTTAGAGCT CCTCCTTATC TTCCCCTTCG CTTCATGGCT 
TCCTCAACCT CCTTTTTCCC TTCCTCTATA CACCTTCGCT TTCTTTCTTC CTCCGCCTCT 
TTCGCTCCTC GCTTCAGTTT CCTTCAGCTC AGGGGGGCGA GATTTCGAAC GATGAAGATT 
TCCACTAGTA TTCGACTGGT ACCAAATGCA GAAGTTTCTT CTAAGGCTTT CATGTCATCA 
AGTGCTGCTA CCGAAACATC AACGAGAAGT GAGAGTTCGA AGGCTTATGG GTCTGAGCAA 
ATTCAGGTTC TGGAAGGCTT AGACCCTGTA AGAAAGAGGC CTGGAATGTA TATTGGGAGC 
ACAGGACCTC GTGGACTACA TCATTTGGTC TATGAAATAC TGGATAACGC TGTTGATGAG 
GCTCAAGCTG GTTTTGCCTC ACAGATAGAT GTTGTCTTGC ATGCAGATAA TTCTGTGAGC 
GTCGCTGACA ATGGACGTGG GATTCCTACT GATTTGCATC CTGTTACGAA GAAGTCTTCC 
TTGGAGACTG TGTTGACGGT ATTGCATGCA GGTGGAAAAT TTGGTGGTTC CAGCAGTGGC 
TACAGTGTCT CAGGCGGACT ACATGGCGTG GGTCTGTCTG TGGTTAATGC CTTGTCTGAG 
TCCTTGGAAG TTTCTATTTG GCGTGATGGG AATGAGTTCC ACCAAAAGTA TTCTCGCGGA 
AAGCCTGCTT CCATTCTAAC ATCCCATGTT CCTTCATCTG AATTGAAAAA TCACCAAGGA 
ACACACATCA GATTTTGGCC TGACAAAGAA GTCTTTACTA CTGGAATACA ATTTGATTAC 
AACACAATAT CTGGACGAAT TAGGGAGCTA GCTTTTCTAA ACCCCAAGCT GACTATAACC 
CTCACAAAAG AGGATAATGA TTTGGAGAAG AACCAGTACA ATGAATACTG CTATGCTGGT 
GGCTTGGTCG AATATGTTGC ATGGCTGAAC AGTGATAAGA ATCCAATTCA TGAAGTCTTT 
GGTTTCAGAA AAGAAACAGA TGGCGTAGCA ATTGATGTCG CGCTTCAGTG GTGTGCAGAT 
GCATATTCAG ACACAATTCT AGGATATGCT AATAGCATAC GGACTATTGA TGGTGGCACC 
CATATAGATG GGATGAAGGC TTCACTGACG AGAACACTCA ATAATCTTGG AAAGAAATCA 
AAAATTTTTA AGGATAAGGA TATCAGCTTA AGTGGTGAAC ATGTTAGGGA GGGATTAACC 
TGTGTTGTCT CAGTCAGGGT CCGAAATCCG GAGTTTGAAG GACAGACCAA GACCAGGTTG 
GGAAATCCTG AAGTGCGAAA AGTAGTTGAG CAATCTATAC AAGAGTACCT AACTGAATAT 
CTGGAGTTGC ACCCTGATGT TCTTGAATTA ATCCTTTCAA AATCTTTAAA TGCTCTCAAG 
GCAGCGTTAG CTGCTAAGAG GGCAAGGGAG CTTGTGAGAC AAAAAAGTGT TTTGCGTTCA 
TCATCCCTTC CCGGCAAGCT AGCTGACTGT TCATCAATCA AACCTGAGGA GTCAGAAATT 
TTCATTGTTG AAGGAGACTC TGCCGGTGGA AGTGCAAAAC AGGGTCGTGA TAGGCGCTTC 
CAGGCTATTC TACCTTTGAG AGGCAAAATC TTAAACATTG AGAGGAAAGA CGAGGCTGCA 
ATGTACAAAA ATGAAGAGAT TAATAATTTA ATTCGTGCTC TTGGACTTGG ATTCAAGGGG 
GAAGATTTTA AAAAGGACGC TCTTCGCTAT CATAAAATAA TCATATTAAC GGATGCTGAT 
GTTGATGGTG CTCACATCCG AACTCTGCTC TTAACGTTTT TCTTCCGATA TCAGAAAGCT 
TTGTTTGACG AAGGGTGCAT TTATGTTGGT GTTCCACCTC TTTACAAGGT TGAGAGGGGG 
AAGCAAGTAT ACTACTGCTA CGACGACAAG GAACTAAAGA AGGTTCAAAG TTCCTTCCCA 
TCAAATGCAT CATATAATAT TCAGAGGTTC AAAGGTTTGG GTGAGATGAT GCCCGCACAA 
CTATGGGAAA CGACGATGGA CCCAGAACGA AGGATGCTGA AGCAGTTGGC AGTTGAGGAT 
GCAGCAGAAG CAAATGTCGT ATTTTCATCC CTTATGGGTG TCCGGGTGGA TTTTCGAAAG 
GAACTTATTC AGAAATCAGC AAGTACGATG AATCTTGAGC ATCTAGATAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101219577 ( XM_004144285.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004144333.2 Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.

Price & Availability↑

CloneID	OCu64601
Clone ID Related Accession (Same CDS sequence)	XM_004144285.2 , XM_004144285.3
Accession Version	XM_004144285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	DNA gyrase subunit B, chloroplastic/mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026658.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004144285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTC GCCAGCTTCT TCTTAGAGCT CCTCCTTATC TTCCCCTTCG CTTCATGGCT 
TCCTCAACCT CCTTTTTCCC TTCCTCTATA CACCTTCGCT TTCTTTCTTC CTCCGCCTCT 
TTCGCTCCTC GCTTCAGTTT CCTTCAGCTC AGGGGGGCGA GATTTCGAAC GATGAAGATT 
TCCACTAGTA TTCGACTGGT ACCAAATGCA GAAGTTTCTT CTAAGGCTTT CATGTCATCA 
AGTGCTGCTA CCGAAACATC AACGAGAAGT GAGAGTTCGA AGGCTTATGG GTCTGAGCAA 
ATTCAGGTTC TGGAAGGCTT AGACCCTGTA AGAAAGAGGC CTGGAATGTA TATTGGGAGC 
ACAGGACCTC GTGGACTACA TCATTTGGTC TATGAAATAC TGGATAACGC TGTTGATGAG 
GCTCAAGCTG GTTTTGCCTC ACAGATAGAT GTTGTCTTGC ATGCAGATAA TTCTGTGAGC 
GTCGCTGACA ATGGACGTGG GATTCCTACT GATTTGCATC CTGTTACGAA GAAGTCTTCC 
TTGGAGACTG TGTTGACGGT ATTGCATGCA GGTGGAAAAT TTGGTGGTTC CAGCAGTGGC 
TACAGTGTCT CAGGCGGACT ACATGGCGTG GGTCTGTCTG TGGTTAATGC CTTGTCTGAG 
TCCTTGGAAG TTTCTATTTG GCGTGATGGG AATGAGTTCC ACCAAAAGTA TTCTCGCGGA 
AAGCCTGCTT CCATTCTAAC ATCCCATGTT CCTTCATCTG AATTGAAAAA TCACCAAGGA 
ACACACATCA GATTTTGGCC TGACAAAGAA GTCTTTACTA CTGGAATACA ATTTGATTAC 
AACACAATAT CTGGACGAAT TAGGGAGCTA GCTTTTCTAA ACCCCAAGCT GACTATAACC 
CTCACAAAAG AGGATAATGA TTTGGAGAAG AACCAGTACA ATGAATACTG CTATGCTGGT 
GGCTTGGTCG AATATGTTGC ATGGCTGAAC AGTGATAAGA ATCCAATTCA TGAAGTCTTT 
GGTTTCAGAA AAGAAACAGA TGGCGTAGCA ATTGATGTCG CGCTTCAGTG GTGTGCAGAT 
GCATATTCAG ACACAATTCT AGGATATGCT AATAGCATAC GGACTATTGA TGGTGGCACC 
CATATAGATG GGATGAAGGC TTCACTGACG AGAACACTCA ATAATCTTGG AAAGAAATCA 
AAAATTTTTA AGGATAAGGA TATCAGCTTA AGTGGTGAAC ATGTTAGGGA GGGATTAACC 
TGTGTTGTCT CAGTCAGGGT CCGAAATCCG GAGTTTGAAG GACAGACCAA GACCAGGTTG 
GGAAATCCTG AAGTGCGAAA AGTAGTTGAG CAATCTATAC AAGAGTACCT AACTGAATAT 
CTGGAGTTGC ACCCTGATGT TCTTGAATTA ATCCTTTCAA AATCTTTAAA TGCTCTCAAG 
GCAGCGTTAG CTGCTAAGAG GGCAAGGGAG CTTGTGAGAC AAAAAAGTGT TTTGCGTTCA 
TCATCCCTTC CCGGCAAGCT AGCTGACTGT TCATCAATCA AACCTGAGGA GTCAGAAATT 
TTCATTGTTG AAGGAGACTC TGCCGGTGGA AGTGCAAAAC AGGGTCGTGA TAGGCGCTTC 
CAGGCTATTC TACCTTTGAG AGGCAAAATC TTAAACATTG AGAGGAAAGA CGAGGCTGCA 
ATGTACAAAA ATGAAGAGAT TAATAATTTA ATTCGTGCTC TTGGACTTGG ATTCAAGGGG 
GAAGATTTTA AAAAGGACGC TCTTCGCTAT CATAAAATAA TCATATTAAC GGATGCTGAT 
GTTGATGGTG CTCACATCCG AACTCTGCTC TTAACGTTTT TCTTCCGATA TCAGAAAGCT 
TTGTTTGACG AAGGGTGCAT TTATGTTGGT GTTCCACCTC TTTACAAGGT TGAGAGGGGG 
AAGCAAGTAT ACTACTGCTA CGACGACAAG GAACTAAAGA AGGTTCAAAG TTCCTTCCCA 
TCAAATGCAT CATATAATAT TCAGAGGTTC AAAGGTTTGG GTGAGATGAT GCCCGCACAA 
CTATGGGAAA CGACGATGGA CCCAGAACGA AGGATGCTGA AGCAGTTGGC AGTTGAGGAT 
GCAGCAGAAG CAAATGTCGT ATTTTCATCC CTTATGGGTG TCCGGGTGGA TTTTCGAAAG 
GAACTTATTC AGAAATCAGC AAGTACGATG AATCTTGAGC ATCTAGATAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004144333.2
CDS	71..2284
Translation	MALRQLLLRAPPYLPLRFMASSTSFFPSSIHLRFLSSSASFAPRFSFLQLRGARFRTMKISTSIRLVPNAEVSSKAFMSSSAATETSTRSESSKAYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSVADNGRGIPTDLHPVTKKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGNEFHQKYSRGKPASILTSHVPSSELKNHQGTHIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPKLTITLTKEDNDLEKNQYNEYCYAGGLVEYVAWLNSDKNPIHEVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIFKDKDISLSGEHVREGLTCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLELILSKSLNALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKDALRYHKIIILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQVYYCYDDKELKKVQSSFPSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGVRVDFRKELIQKSASTMNLEHLDI

Target ORF information:

RefSeq Version	XM_004144285.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus DNA gyrase subunit B, chloroplastic/mitochondrial (LOC101219577), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004144285.3

ORF Insert Sequence:

ATGGCGCTTC GCCAGCTTCT TCTTAGAGCT CCTCCTTATC TTCCCCTTCG CTTCATGGCT 
TCCTCAACCT CCTTTTTCCC TTCCTCTATA CACCTTCGCT TTCTTTCTTC CTCCGCCTCT 
TTCGCTCCTC GCTTCAGTTT CCTTCAGCTC AGGGGGGCGA GATTTCGAAC GATGAAGATT 
TCCACTAGTA TTCGACTGGT ACCAAATGCA GAAGTTTCTT CTAAGGCTTT CATGTCATCA 
AGTGCTGCTA CCGAAACATC AACGAGAAGT GAGAGTTCGA AGGCTTATGG GTCTGAGCAA 
ATTCAGGTTC TGGAAGGCTT AGACCCTGTA AGAAAGAGGC CTGGAATGTA TATTGGGAGC 
ACAGGACCTC GTGGACTACA TCATTTGGTC TATGAAATAC TGGATAACGC TGTTGATGAG 
GCTCAAGCTG GTTTTGCCTC ACAGATAGAT GTTGTCTTGC ATGCAGATAA TTCTGTGAGC 
GTCGCTGACA ATGGACGTGG GATTCCTACT GATTTGCATC CTGTTACGAA GAAGTCTTCC 
TTGGAGACTG TGTTGACGGT ATTGCATGCA GGTGGAAAAT TTGGTGGTTC CAGCAGTGGC 
TACAGTGTCT CAGGCGGACT ACATGGCGTG GGTCTGTCTG TGGTTAATGC CTTGTCTGAG 
TCCTTGGAAG TTTCTATTTG GCGTGATGGG AATGAGTTCC ACCAAAAGTA TTCTCGCGGA 
AAGCCTGCTT CCATTCTAAC ATCCCATGTT CCTTCATCTG AATTGAAAAA TCACCAAGGA 
ACACACATCA GATTTTGGCC TGACAAAGAA GTCTTTACTA CTGGAATACA ATTTGATTAC 
AACACAATAT CTGGACGAAT TAGGGAGCTA GCTTTTCTAA ACCCCAAGCT GACTATAACC 
CTCACAAAAG AGGATAATGA TTTGGAGAAG AACCAGTACA ATGAATACTG CTATGCTGGT 
GGCTTGGTCG AATATGTTGC ATGGCTGAAC AGTGATAAGA ATCCAATTCA TGAAGTCTTT 
GGTTTCAGAA AAGAAACAGA TGGCGTAGCA ATTGATGTCG CGCTTCAGTG GTGTGCAGAT 
GCATATTCAG ACACAATTCT AGGATATGCT AATAGCATAC GGACTATTGA TGGTGGCACC 
CATATAGATG GGATGAAGGC TTCACTGACG AGAACACTCA ATAATCTTGG AAAGAAATCA 
AAAATTTTTA AGGATAAGGA TATCAGCTTA AGTGGTGAAC ATGTTAGGGA GGGATTAACC 
TGTGTTGTCT CAGTCAGGGT CCGAAATCCG GAGTTTGAAG GACAGACCAA GACCAGGTTG 
GGAAATCCTG AAGTGCGAAA AGTAGTTGAG CAATCTATAC AAGAGTACCT AACTGAATAT 
CTGGAGTTGC ACCCTGATGT TCTTGAATTA ATCCTTTCAA AATCTTTAAA TGCTCTCAAG 
GCAGCGTTAG CTGCTAAGAG GGCAAGGGAG CTTGTGAGAC AAAAAAGTGT TTTGCGTTCA 
TCATCCCTTC CCGGCAAGCT AGCTGACTGT TCATCAATCA AACCTGAGGA GTCAGAAATT 
TTCATTGTTG AAGGAGACTC TGCCGGTGGA AGTGCAAAAC AGGGTCGTGA TAGGCGCTTC 
CAGGCTATTC TACCTTTGAG AGGCAAAATC TTAAACATTG AGAGGAAAGA CGAGGCTGCA 
ATGTACAAAA ATGAAGAGAT TAATAATTTA ATTCGTGCTC TTGGACTTGG ATTCAAGGGG 
GAAGATTTTA AAAAGGACGC TCTTCGCTAT CATAAAATAA TCATATTAAC GGATGCTGAT 
GTTGATGGTG CTCACATCCG AACTCTGCTC TTAACGTTTT TCTTCCGATA TCAGAAAGCT 
TTGTTTGACG AAGGGTGCAT TTATGTTGGT GTTCCACCTC TTTACAAGGT TGAGAGGGGG 
AAGCAAGTAT ACTACTGCTA CGACGACAAG GAACTAAAGA AGGTTCAAAG TTCCTTCCCA 
TCAAATGCAT CATATAATAT TCAGAGGTTC AAAGGTTTGG GTGAGATGAT GCCCGCACAA 
CTATGGGAAA CGACGATGGA CCCAGAACGA AGGATGCTGA AGCAGTTGGC AGTTGAGGAT 
GCAGCAGAAG CAAATGTCGT ATTTTCATCC CTTATGGGTG TCCGGGTGGA TTTTCGAAAG 
GAACTTATTC AGAAATCAGC AAGTACGATG AATCTTGAGC ATCTAGATAT TTAA