LOC101209889 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101209889
Entrez Gene ID	101209889
Full Name	nucleolin 1
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011653481.1	XP_011651783.1	nucleolin 1 isoform X1
XM_011653483.2	XP_011651785.1	nucleolin 1 isoform X3
XM_011653482.1	XP_011651784.1	nucleolin 1 isoform X2
XM_011653481.2	XP_011651783.1	nucleolin 1 isoform X1
XM_011653482.2	XP_011651784.1	nucleolin 1 isoform X2
XM_011653483.1	XP_011651785.1	nucleolin 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101209889 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101209889 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu60913	XM_011653481.2 Latest version!	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCu60914	XM_011653482.2 Latest version!	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCu60915	XM_011653483.2 Latest version!	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCu60913	XM_011653481.1	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCu60914	XM_011653482.1	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCu60915	XM_011653483.1	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101209889 ( XM_011653481.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651783.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu60913
Clone ID Related Accession (Same CDS sequence)	XM_011653481.1 , XM_011653481.2
Accession Version	XM_011653481.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG TAAATATCTC TGTGAATGTT AATACTTGGA AACCACCTCA CTATCAGGAC 
CAAGGGCAAA TTCCTGAACT TGCTGGTCAA AAGTTTTTGC AGGAGAGTGA GTTACAGGGT 
ACCAGATCGG CTGTGCAAGT TATGGAGAAT GGCGAGCCAG TGACCCTTGT TAACCAACGG 
AAAACACCAG CATCTAACCT TCAAGAAGAG TTCCAGAAAC CTCCTTTATC TGCTAATGGC 
CAATTTGCTG GCACTCGTCC TTTAGAGGAA GCTGATGCTC GAACCATTTT TGTGAGCAAT 
GTTCACTTTG CTGCAACGAA GGATAGCCTG TCTAGGCATT TTAACAAGTT TGGAGAAGTT 
TTAAAAGTCA TCATAGTAAC CGATGCAACC ACTGGGCAAC CCAAAGGGTC AGCTTATGTG 
GAGTTCATGA GAAAAGAATC TGCAGAGAAT GCGTTGTCTC TTGATGGGAC CTCGTTTATG 
TCTAGGATTC TAAAGGTCGT AAGGAAAAAT GCTTCACAAT TAGAAGGTGC TTCAACTGTT 
ATCTGGCCTC GTGCTGTGCG AGGCTCGCCG TTTCCTACTT CGAGATTTAC CAGAGCTCCA 
TTCATGAGGG GTGTCCCTGG TGGATTTAGG GCTCGTCCCC CGATGAAACT CAGGGCCAGG 
AGCATGCAGT GGAAGCGTGA TAATCAGACC ACAACTGCTG ACAATGGTGC ATCTCTCTCT 
GGTAATTCTA TTCCCTCACC TGGTGCTCGT AGTCTCACCT ACGTTCGAAC AGAACCTAAG 
CCAGTGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651783.1
CDS	250..2304
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIVNISVNVNTWKPPHYQDQGQIPELAGQKFLQESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653481.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653481.1

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG TAAATATCTC TGTGAATGTT AATACTTGGA AACCACCTCA CTATCAGGAC 
CAAGGGCAAA TTCCTGAACT TGCTGGTCAA AAGTTTTTGC AGGAGAGTGA GTTACAGGGT 
ACCAGATCGG CTGTGCAAGT TATGGAGAAT GGCGAGCCAG TGACCCTTGT TAACCAACGG 
AAAACACCAG CATCTAACCT TCAAGAAGAG TTCCAGAAAC CTCCTTTATC TGCTAATGGC 
CAATTTGCTG GCACTCGTCC TTTAGAGGAA GCTGATGCTC GAACCATTTT TGTGAGCAAT 
GTTCACTTTG CTGCAACGAA GGATAGCCTG TCTAGGCATT TTAACAAGTT TGGAGAAGTT 
TTAAAAGTCA TCATAGTAAC CGATGCAACC ACTGGGCAAC CCAAAGGGTC AGCTTATGTG 
GAGTTCATGA GAAAAGAATC TGCAGAGAAT GCGTTGTCTC TTGATGGGAC CTCGTTTATG 
TCTAGGATTC TAAAGGTCGT AAGGAAAAAT GCTTCACAAT TAGAAGGTGC TTCAACTGTT 
ATCTGGCCTC GTGCTGTGCG AGGCTCGCCG TTTCCTACTT CGAGATTTAC CAGAGCTCCA 
TTCATGAGGG GTGTCCCTGG TGGATTTAGG GCTCGTCCCC CGATGAAACT CAGGGCCAGG 
AGCATGCAGT GGAAGCGTGA TAATCAGACC ACAACTGCTG ACAATGGTGC ATCTCTCTCT 
GGTAATTCTA TTCCCTCACC TGGTGCTCGT AGTCTCACCT ACGTTCGAAC AGAACCTAAG 
CCAGTGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209889 ( XM_011653481.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651783.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu60913
Clone ID Related Accession (Same CDS sequence)	XM_011653481.1 , XM_011653481.2
Accession Version	XM_011653481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011653481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG TAAATATCTC TGTGAATGTT AATACTTGGA AACCACCTCA CTATCAGGAC 
CAAGGGCAAA TTCCTGAACT TGCTGGTCAA AAGTTTTTGC AGGAGAGTGA GTTACAGGGT 
ACCAGATCGG CTGTGCAAGT TATGGAGAAT GGCGAGCCAG TGACCCTTGT TAACCAACGG 
AAAACACCAG CATCTAACCT TCAAGAAGAG TTCCAGAAAC CTCCTTTATC TGCTAATGGC 
CAATTTGCTG GCACTCGTCC TTTAGAGGAA GCTGATGCTC GAACCATTTT TGTGAGCAAT 
GTTCACTTTG CTGCAACGAA GGATAGCCTG TCTAGGCATT TTAACAAGTT TGGAGAAGTT 
TTAAAAGTCA TCATAGTAAC CGATGCAACC ACTGGGCAAC CCAAAGGGTC AGCTTATGTG 
GAGTTCATGA GAAAAGAATC TGCAGAGAAT GCGTTGTCTC TTGATGGGAC CTCGTTTATG 
TCTAGGATTC TAAAGGTCGT AAGGAAAAAT GCTTCACAAT TAGAAGGTGC TTCAACTGTT 
ATCTGGCCTC GTGCTGTGCG AGGCTCGCCG TTTCCTACTT CGAGATTTAC CAGAGCTCCA 
TTCATGAGGG GTGTCCCTGG TGGATTTAGG GCTCGTCCCC CGATGAAACT CAGGGCCAGG 
AGCATGCAGT GGAAGCGTGA TAATCAGACC ACAACTGCTG ACAATGGTGC ATCTCTCTCT 
GGTAATTCTA TTCCCTCACC TGGTGCTCGT AGTCTCACCT ACGTTCGAAC AGAACCTAAG 
CCAGTGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651783.1
CDS	279..2333
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIVNISVNVNTWKPPHYQDQGQIPELAGQKFLQESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653481.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653481.2

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG TAAATATCTC TGTGAATGTT AATACTTGGA AACCACCTCA CTATCAGGAC 
CAAGGGCAAA TTCCTGAACT TGCTGGTCAA AAGTTTTTGC AGGAGAGTGA GTTACAGGGT 
ACCAGATCGG CTGTGCAAGT TATGGAGAAT GGCGAGCCAG TGACCCTTGT TAACCAACGG 
AAAACACCAG CATCTAACCT TCAAGAAGAG TTCCAGAAAC CTCCTTTATC TGCTAATGGC 
CAATTTGCTG GCACTCGTCC TTTAGAGGAA GCTGATGCTC GAACCATTTT TGTGAGCAAT 
GTTCACTTTG CTGCAACGAA GGATAGCCTG TCTAGGCATT TTAACAAGTT TGGAGAAGTT 
TTAAAAGTCA TCATAGTAAC CGATGCAACC ACTGGGCAAC CCAAAGGGTC AGCTTATGTG 
GAGTTCATGA GAAAAGAATC TGCAGAGAAT GCGTTGTCTC TTGATGGGAC CTCGTTTATG 
TCTAGGATTC TAAAGGTCGT AAGGAAAAAT GCTTCACAAT TAGAAGGTGC TTCAACTGTT 
ATCTGGCCTC GTGCTGTGCG AGGCTCGCCG TTTCCTACTT CGAGATTTAC CAGAGCTCCA 
TTCATGAGGG GTGTCCCTGG TGGATTTAGG GCTCGTCCCC CGATGAAACT CAGGGCCAGG 
AGCATGCAGT GGAAGCGTGA TAATCAGACC ACAACTGCTG ACAATGGTGC ATCTCTCTCT 
GGTAATTCTA TTCCCTCACC TGGTGCTCGT AGTCTCACCT ACGTTCGAAC AGAACCTAAG 
CCAGTGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209889 ( XM_011653482.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651784.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCu60914
Clone ID Related Accession (Same CDS sequence)	XM_011653482.1 , XM_011653482.2
Accession Version	XM_011653482.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTT TTTTGCAGGA GAGTGAGTTA CAGGGTACCA GATCGGCTGT GCAAGTTATG 
GAGAATGGCG AGCCAGTGAC CCTTGTTAAC CAACGGAAAA CACCAGCATC TAACCTTCAA 
GAAGAGTTCC AGAAACCTCC TTTATCTGCT AATGGCCAAT TTGCTGGCAC TCGTCCTTTA 
GAGGAAGCTG ATGCTCGAAC CATTTTTGTG AGCAATGTTC ACTTTGCTGC AACGAAGGAT 
AGCCTGTCTA GGCATTTTAA CAAGTTTGGA GAAGTTTTAA AAGTCATCAT AGTAACCGAT 
GCAACCACTG GGCAACCCAA AGGGTCAGCT TATGTGGAGT TCATGAGAAA AGAATCTGCA 
GAGAATGCGT TGTCTCTTGA TGGGACCTCG TTTATGTCTA GGATTCTAAA GGTCGTAAGG 
AAAAATGCTT CACAATTAGA AGGTGCTTCA ACTGTTATCT GGCCTCGTGC TGTGCGAGGC 
TCGCCGTTTC CTACTTCGAG ATTTACCAGA GCTCCATTCA TGAGGGGTGT CCCTGGTGGA 
TTTAGGGCTC GTCCCCCGAT GAAACTCAGG GCCAGGAGCA TGCAGTGGAA GCGTGATAAT 
CAGACCACAA CTGCTGACAA TGGTGCATCT CTCTCTGGTA ATTCTATTCC CTCACCTGGT 
GCTCGTAGTC TCACCTACGT TCGAACAGAA CCTAAGCCAG TGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651784.1
CDS	250..2220
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIFLQESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653482.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653482.1

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTT TTTTGCAGGA GAGTGAGTTA CAGGGTACCA GATCGGCTGT GCAAGTTATG 
GAGAATGGCG AGCCAGTGAC CCTTGTTAAC CAACGGAAAA CACCAGCATC TAACCTTCAA 
GAAGAGTTCC AGAAACCTCC TTTATCTGCT AATGGCCAAT TTGCTGGCAC TCGTCCTTTA 
GAGGAAGCTG ATGCTCGAAC CATTTTTGTG AGCAATGTTC ACTTTGCTGC AACGAAGGAT 
AGCCTGTCTA GGCATTTTAA CAAGTTTGGA GAAGTTTTAA AAGTCATCAT AGTAACCGAT 
GCAACCACTG GGCAACCCAA AGGGTCAGCT TATGTGGAGT TCATGAGAAA AGAATCTGCA 
GAGAATGCGT TGTCTCTTGA TGGGACCTCG TTTATGTCTA GGATTCTAAA GGTCGTAAGG 
AAAAATGCTT CACAATTAGA AGGTGCTTCA ACTGTTATCT GGCCTCGTGC TGTGCGAGGC 
TCGCCGTTTC CTACTTCGAG ATTTACCAGA GCTCCATTCA TGAGGGGTGT CCCTGGTGGA 
TTTAGGGCTC GTCCCCCGAT GAAACTCAGG GCCAGGAGCA TGCAGTGGAA GCGTGATAAT 
CAGACCACAA CTGCTGACAA TGGTGCATCT CTCTCTGGTA ATTCTATTCC CTCACCTGGT 
GCTCGTAGTC TCACCTACGT TCGAACAGAA CCTAAGCCAG TGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209889 ( XM_011653482.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651784.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.

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CloneID	OCu60914
Clone ID Related Accession (Same CDS sequence)	XM_011653482.1 , XM_011653482.2
Accession Version	XM_011653482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011653482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTT TTTTGCAGGA GAGTGAGTTA CAGGGTACCA GATCGGCTGT GCAAGTTATG 
GAGAATGGCG AGCCAGTGAC CCTTGTTAAC CAACGGAAAA CACCAGCATC TAACCTTCAA 
GAAGAGTTCC AGAAACCTCC TTTATCTGCT AATGGCCAAT TTGCTGGCAC TCGTCCTTTA 
GAGGAAGCTG ATGCTCGAAC CATTTTTGTG AGCAATGTTC ACTTTGCTGC AACGAAGGAT 
AGCCTGTCTA GGCATTTTAA CAAGTTTGGA GAAGTTTTAA AAGTCATCAT AGTAACCGAT 
GCAACCACTG GGCAACCCAA AGGGTCAGCT TATGTGGAGT TCATGAGAAA AGAATCTGCA 
GAGAATGCGT TGTCTCTTGA TGGGACCTCG TTTATGTCTA GGATTCTAAA GGTCGTAAGG 
AAAAATGCTT CACAATTAGA AGGTGCTTCA ACTGTTATCT GGCCTCGTGC TGTGCGAGGC 
TCGCCGTTTC CTACTTCGAG ATTTACCAGA GCTCCATTCA TGAGGGGTGT CCCTGGTGGA 
TTTAGGGCTC GTCCCCCGAT GAAACTCAGG GCCAGGAGCA TGCAGTGGAA GCGTGATAAT 
CAGACCACAA CTGCTGACAA TGGTGCATCT CTCTCTGGTA ATTCTATTCC CTCACCTGGT 
GCTCGTAGTC TCACCTACGT TCGAACAGAA CCTAAGCCAG TGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651784.1
CDS	279..2249
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIFLQESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653482.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653482.2

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTT TTTTGCAGGA GAGTGAGTTA CAGGGTACCA GATCGGCTGT GCAAGTTATG 
GAGAATGGCG AGCCAGTGAC CCTTGTTAAC CAACGGAAAA CACCAGCATC TAACCTTCAA 
GAAGAGTTCC AGAAACCTCC TTTATCTGCT AATGGCCAAT TTGCTGGCAC TCGTCCTTTA 
GAGGAAGCTG ATGCTCGAAC CATTTTTGTG AGCAATGTTC ACTTTGCTGC AACGAAGGAT 
AGCCTGTCTA GGCATTTTAA CAAGTTTGGA GAAGTTTTAA AAGTCATCAT AGTAACCGAT 
GCAACCACTG GGCAACCCAA AGGGTCAGCT TATGTGGAGT TCATGAGAAA AGAATCTGCA 
GAGAATGCGT TGTCTCTTGA TGGGACCTCG TTTATGTCTA GGATTCTAAA GGTCGTAAGG 
AAAAATGCTT CACAATTAGA AGGTGCTTCA ACTGTTATCT GGCCTCGTGC TGTGCGAGGC 
TCGCCGTTTC CTACTTCGAG ATTTACCAGA GCTCCATTCA TGAGGGGTGT CCCTGGTGGA 
TTTAGGGCTC GTCCCCCGAT GAAACTCAGG GCCAGGAGCA TGCAGTGGAA GCGTGATAAT 
CAGACCACAA CTGCTGACAA TGGTGCATCT CTCTCTGGTA ATTCTATTCC CTCACCTGGT 
GCTCGTAGTC TCACCTACGT TCGAACAGAA CCTAAGCCAG TGGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209889 ( XM_011653483.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651785.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCu60915
Clone ID Related Accession (Same CDS sequence)	XM_011653483.2 , XM_011653483.1
Accession Version	XM_011653483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011653483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG AGAGTGAGTT ACAGGGTACC AGATCGGCTG TGCAAGTTAT GGAGAATGGC 
GAGCCAGTGA CCCTTGTTAA CCAACGGAAA ACACCAGCAT CTAACCTTCA AGAAGAGTTC 
CAGAAACCTC CTTTATCTGC TAATGGCCAA TTTGCTGGCA CTCGTCCTTT AGAGGAAGCT 
GATGCTCGAA CCATTTTTGT GAGCAATGTT CACTTTGCTG CAACGAAGGA TAGCCTGTCT 
AGGCATTTTA ACAAGTTTGG AGAAGTTTTA AAAGTCATCA TAGTAACCGA TGCAACCACT 
GGGCAACCCA AAGGGTCAGC TTATGTGGAG TTCATGAGAA AAGAATCTGC AGAGAATGCG 
TTGTCTCTTG ATGGGACCTC GTTTATGTCT AGGATTCTAA AGGTCGTAAG GAAAAATGCT 
TCACAATTAG AAGGTGCTTC AACTGTTATC TGGCCTCGTG CTGTGCGAGG CTCGCCGTTT 
CCTACTTCGA GATTTACCAG AGCTCCATTC ATGAGGGGTG TCCCTGGTGG ATTTAGGGCT 
CGTCCCCCGA TGAAACTCAG GGCCAGGAGC ATGCAGTGGA AGCGTGATAA TCAGACCACA 
ACTGCTGACA ATGGTGCATC TCTCTCTGGT AATTCTATTC CCTCACCTGG TGCTCGTAGT 
CTCACCTACG TTCGAACAGA ACCTAAGCCA GTGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651785.1
CDS	279..2240
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653483.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653483.2

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG AGAGTGAGTT ACAGGGTACC AGATCGGCTG TGCAAGTTAT GGAGAATGGC 
GAGCCAGTGA CCCTTGTTAA CCAACGGAAA ACACCAGCAT CTAACCTTCA AGAAGAGTTC 
CAGAAACCTC CTTTATCTGC TAATGGCCAA TTTGCTGGCA CTCGTCCTTT AGAGGAAGCT 
GATGCTCGAA CCATTTTTGT GAGCAATGTT CACTTTGCTG CAACGAAGGA TAGCCTGTCT 
AGGCATTTTA ACAAGTTTGG AGAAGTTTTA AAAGTCATCA TAGTAACCGA TGCAACCACT 
GGGCAACCCA AAGGGTCAGC TTATGTGGAG TTCATGAGAA AAGAATCTGC AGAGAATGCG 
TTGTCTCTTG ATGGGACCTC GTTTATGTCT AGGATTCTAA AGGTCGTAAG GAAAAATGCT 
TCACAATTAG AAGGTGCTTC AACTGTTATC TGGCCTCGTG CTGTGCGAGG CTCGCCGTTT 
CCTACTTCGA GATTTACCAG AGCTCCATTC ATGAGGGGTG TCCCTGGTGG ATTTAGGGCT 
CGTCCCCCGA TGAAACTCAG GGCCAGGAGC ATGCAGTGGA AGCGTGATAA TCAGACCACA 
ACTGCTGACA ATGGTGCATC TCTCTCTGGT AATTCTATTC CCTCACCTGG TGCTCGTAGT 
CTCACCTACG TTCGAACAGA ACCTAAGCCA GTGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209889 ( XM_011653483.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011651785.1 Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.

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CloneID	OCu60915
Clone ID Related Accession (Same CDS sequence)	XM_011653483.2 , XM_011653483.1
Accession Version	XM_011653483.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	nucleolin 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG AGAGTGAGTT ACAGGGTACC AGATCGGCTG TGCAAGTTAT GGAGAATGGC 
GAGCCAGTGA CCCTTGTTAA CCAACGGAAA ACACCAGCAT CTAACCTTCA AGAAGAGTTC 
CAGAAACCTC CTTTATCTGC TAATGGCCAA TTTGCTGGCA CTCGTCCTTT AGAGGAAGCT 
GATGCTCGAA CCATTTTTGT GAGCAATGTT CACTTTGCTG CAACGAAGGA TAGCCTGTCT 
AGGCATTTTA ACAAGTTTGG AGAAGTTTTA AAAGTCATCA TAGTAACCGA TGCAACCACT 
GGGCAACCCA AAGGGTCAGC TTATGTGGAG TTCATGAGAA AAGAATCTGC AGAGAATGCG 
TTGTCTCTTG ATGGGACCTC GTTTATGTCT AGGATTCTAA AGGTCGTAAG GAAAAATGCT 
TCACAATTAG AAGGTGCTTC AACTGTTATC TGGCCTCGTG CTGTGCGAGG CTCGCCGTTT 
CCTACTTCGA GATTTACCAG AGCTCCATTC ATGAGGGGTG TCCCTGGTGG ATTTAGGGCT 
CGTCCCCCGA TGAAACTCAG GGCCAGGAGC ATGCAGTGGA AGCGTGATAA TCAGACCACA 
ACTGCTGACA ATGGTGCATC TCTCTCTGGT AATTCTATTC CCTCACCTGG TGCTCGTAGT 
CTCACCTACG TTCGAACAGA ACCTAAGCCA GTGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011651785.1
CDS	250..2211
Translation	MNSSEMGSEDRVDDRTFKVDFTGDGMAKLRERIKLKMKEFMGDYTDDTLVEYVIVLLRNGRRREEAQNELNVFLADDSHSFVSWLWDHLASSMDLYVEPPAKASANEVPTQKSPVAEPDTRNNSHNLESDSERGKTEKLSSRRRTREWRGIANEETRVVPRSEVSRVKHSSPEQVPGHRKRSRPDDHQGTEREAAFQVSIAAPRRLLQFAMRDAVATTRPSNASKEPHSKRLRSVVSASNSDTTNRPRRLQSVAKVPNPMATVIKAVTEASEDVVRVKSSSVFDRLGRQSRDMDLTESSGRPSEYGATFVENDEYGDMNHSEDRPYSATYLERGNYIGKYSRDEPMFEPETGLASDSTSENEDVNIQGHRVFDDSWTAESGVRKGSNLRTVAFRAVENSDDERVAQYTQKDQPSLAANSSRDIESELQGTRSAVQVMENGEPVTLVNQRKTPASNLQEEFQKPPLSANGQFAGTRPLEEADARTIFVSNVHFAATKDSLSRHFNKFGEVLKVIIVTDATTGQPKGSAYVEFMRKESAENALSLDGTSFMSRILKVVRKNASQLEGASTVIWPRAVRGSPFPTSRFTRAPFMRGVPGGFRARPPMKLRARSMQWKRDNQTTTADNGASLSGNSIPSPGARSLTYVRTEPKPVDK

Target ORF information:

RefSeq Version	XM_011653483.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus nucleolin 1 (LOC101209889), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011653483.1

ORF Insert Sequence:

ATGAACTCGT CGGAGATGGG GAGTGAGGAT CGTGTCGATG ATCGTACGTT CAAGGTTGAT 
TTTACTGGCG ACGGAATGGC GAAGCTCAGA GAGAGGATAA AGCTGAAGAT GAAGGAATTC 
ATGGGCGATT ATACTGACGA CACTCTCGTG GAATACGTGA TTGTCTTGTT GAGGAACGGC 
AGGCGCAGAG AAGAAGCACA GAATGAACTT AATGTCTTTT TGGCAGACGA TAGTCATTCA 
TTTGTATCTT GGTTGTGGGA CCATCTGGCT TCAAGTATGG ATTTATATGT GGAGCCGCCT 
GCTAAAGCTT CTGCCAATGA GGTGCCCACA CAAAAGTCTC CAGTAGCAGA ACCAGATACA 
AGAAATAATT CTCACAATCT GGAGTCTGAT TCTGAAAGAG GGAAGACTGA AAAATTGTCT 
AGCAGACGAC GTACTAGGGA ATGGAGAGGA ATTGCTAATG AGGAAACCCG TGTAGTTCCA 
AGATCTGAAG TTAGTCGTGT CAAGCATTCT TCTCCTGAAC AAGTTCCTGG TCATAGGAAA 
AGGAGCCGGC CTGACGATCA TCAAGGCACT GAGAGGGAAG CTGCCTTTCA GGTAAGCATT 
GCTGCCCCTC GACGGCTGCT CCAGTTTGCA ATGCGAGATG CTGTGGCCAC CACGAGGCCC 
TCTAATGCAT CTAAGGAGCC CCATTCAAAA CGTCTTCGTT CAGTAGTTTC TGCATCCAAT 
AGTGACACAA CTAATCGTCC TAGGAGGCTT CAATCTGTTG CAAAAGTTCC AAATCCAATG 
GCAACTGTCA TCAAGGCTGT GACGGAGGCA TCTGAAGATG TGGTCAGAGT TAAATCCTCC 
AGTGTTTTTG ACCGACTTGG TCGTCAATCT CGTGATATGG ATTTAACAGA GTCTAGTGGC 
CGACCTTCAG AATATGGAGC AACTTTTGTA GAGAACGATG AATATGGGGA TATGAATCAT 
TCAGAGGATC GACCATACTC AGCTACCTAT CTTGAGAGAG GCAATTATAT TGGGAAATAT 
TCTCGTGATG AGCCCATGTT TGAACCTGAA ACTGGATTAG CATCAGATTC TACATCTGAA 
AATGAAGATG TTAATATTCA GGGCCACAGA GTATTTGATG ATTCTTGGAC TGCCGAATCA 
GGAGTAAGAA AGGGAAGCAA TTTACGGACT GTGGCATTCA GGGCAGTTGA GAATTCTGAT 
GATGAACGTG TGGCACAATA TACACAGAAG GATCAACCTT CGTTAGCTGC AAATTCCTCA 
CGTGACATTG AGAGTGAGTT ACAGGGTACC AGATCGGCTG TGCAAGTTAT GGAGAATGGC 
GAGCCAGTGA CCCTTGTTAA CCAACGGAAA ACACCAGCAT CTAACCTTCA AGAAGAGTTC 
CAGAAACCTC CTTTATCTGC TAATGGCCAA TTTGCTGGCA CTCGTCCTTT AGAGGAAGCT 
GATGCTCGAA CCATTTTTGT GAGCAATGTT CACTTTGCTG CAACGAAGGA TAGCCTGTCT 
AGGCATTTTA ACAAGTTTGG AGAAGTTTTA AAAGTCATCA TAGTAACCGA TGCAACCACT 
GGGCAACCCA AAGGGTCAGC TTATGTGGAG TTCATGAGAA AAGAATCTGC AGAGAATGCG 
TTGTCTCTTG ATGGGACCTC GTTTATGTCT AGGATTCTAA AGGTCGTAAG GAAAAATGCT 
TCACAATTAG AAGGTGCTTC AACTGTTATC TGGCCTCGTG CTGTGCGAGG CTCGCCGTTT 
CCTACTTCGA GATTTACCAG AGCTCCATTC ATGAGGGGTG TCCCTGGTGG ATTTAGGGCT 
CGTCCCCCGA TGAAACTCAG GGCCAGGAGC ATGCAGTGGA AGCGTGATAA TCAGACCACA 
ACTGCTGACA ATGGTGCATC TCTCTCTGGT AATTCTATTC CCTCACCTGG TGCTCGTAGT 
CTCACCTACG TTCGAACAGA ACCTAAGCCA GTGGACAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101209889 cDNA ORF clone, Cucumis sativus(cucumber)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム