LOC101206845 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101206845
Entrez Gene ID	101206845
Full Name	auxin response factor 2
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004137972.2	XP_004138020.1	auxin response factor 2
XM_004137972.3	XP_004138020.1	auxin response factor 2A

Pathways from BioSystems
Gene Ontology

The following LOC101206845 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101206845 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu52349	XM_004137972.3 Latest version!	Cucumis sativus auxin response factor 2A (LOC101206845), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCu52349	XM_004137972.2	Cucumis sativus auxin response factor 2 (LOC101206845), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101206845 ( XM_004137972.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004138020.1 Cucumis sativus auxin response factor 2 (LOC101206845), mRNA.

Price & Availability↑

CloneID	OCu52349
Clone ID Related Accession (Same CDS sequence)	XM_004137972.2 , XM_004137972.3
Accession Version	XM_004137972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	auxin response factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004137972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCT CCGAGGTTTC GATCAACCCC AATTCCGCCT CCTTCAACGA CCATGCCGAT 
TCTACCAAAG ACACCTCCGA TCCACCCAAC GCTCTCTCTC CCAGGGATGC TGATATCGCT 
CTCTACACCG AGCTCTGGAA TGCTTGTGCT GGTCCTCTCG TTTCTGTTCC TCGTGAAAAC 
GAGCGCGTCT TCTACTTCCC TCAGGGCCAC ATCGAACAGG TGGAAGCATC AACTAGTCAA 
GTGGCGGACC AGCAGATGCC TGTTTATAAC CTTCCATCGA AGATCCTCTG CCGCGTTATT 
AATGTGCACT TAAAGGCCGA GCCAGACACC GATGAGGTAT TTGCACAGAT CACTTTGCTT 
CCAGAGGCTA ATCAAGATGA GCATGCTGTA GACAAGGAAC CTCCACCTCC TCCCCCGAGG 
AGATTTCACG TGCATTCTTT TTGCAAGACT CTGACTGCTT CTGACACTAG TACTCATGGG 
GGGTTTTCAG TCTTGAGACG CCATGCTGAT GAATGTCTTC CACCACTGGA CATGTCACGA 
CAGCCTCCTA CCCAGGAGTT GGTTGCAAAG GATTTGCATG GAAATGAATG GCGTTTCCGT 
CATATATTTC GAGGGCAACC ACGAAGGCAT TTGCTTCAGA GTGGTTGGAG TGTCTTTGTG 
AGCTCAAAAA GACTGGTTGC AGGAGATGCT TTTATATTTT TGAGGGGTGA GAATGGAGAG 
CTGCGTGTTG GTGTGAGGCG TGCAATGAGA CAACATGGCA ATGTTCCATC ATCGGTCATA 
TCTAGCCACA GCATGCATCT TGGTGTGCTT GCGACTGCTT GGCATGCTAT TTCGACAGGC 
ACATTGTTCA CTGTCTACTA TAAGCCTAGG ACAAGCCCTT CTGAATTCAT TGTTCCATAT 
GACCAGTATA TGGAGTCTAT CAAGAAGAGC TACACCATAG GAATGCGGTT TAAAATGAGA 
TTTGAAGGTG AAGAGGCTCC TGAGCAGAGG TTCACTGGTA CTATCATTGG ATGTGAAGAT 
GCTGATCCCA AAAGGTGGAA AGATTCCAAG TGGAGATGCC TGAAGGTGAG ATGGGATGAA 
ACTTCTACTA TTTCTCGTCC AGAGAAAGTC TCACCGTGGA AAATTGAGCC GGCCCTTGCT 
CCTCCTGCGC TGAATCCCCT TCCTATGACT AGACCTAAAA GGCCTCGATC AAACATGGTC 
TCTACATCCC CTGATTCCTC TGTTCTTACA CGAGAAGGCT CATCAAGAGT GACTGTAGAC 
CCTTCACCAG CCAGTGCGTT TACAAGGGTC TTGCAAGGTC AAGAATTCTC GACCTTGAGA 
GGTAATTTTA TCGATGGCAG TGATCCAGAT GCGGCTGAAA AATCTGTTAT GTGGCCTCCT 
TCATTAGATG ATGAGAAAGT TGACGTGGTT TCTACATCTA AGAAACATGG AGCAGATAGC 
TGGATACCTC CAGGAAGGAG TGAGCCAACC TACGCAGATC TACTGTCAGG TTTTGGAACT 
GATATGGATT CTTCTCATGG TGTTCGTGCT GCCATGGGAG ATTCTGCATT AGTCACTGCT 
AATTCAATCA GAAAGCATGC CATGGAACAC GATGGAAAAT TTAGCTTCCT TGGTGGTAGC 
TCATGGTCTG TCTTGCCTTC TGGTCTCTCG CTCAACCTGG TTGACTCCAG TCAGAAGGGT 
CATATTCGGG CTGGTGACTT ATCTTATCAA GTGAGAGGAA ATGCTACCTT CAATGGATTT 
GGCGATCATT CCATATCCCA TTGTCATAGA ACTGAGCAGC CTCATGGAAA TTGGCTGATG 
CCTCCACCTT CATCTCATTT TGACTATCCA ATTCATTCAA GTGAACTGAT GTCAAAACCT 
ATGTTATTTC AGAACCAAGA TATACTGAAA CCCAAAGATG GGAATTGTAA GCTTTTCGGT 
ATTTCCCTTG TTAAAAATCC TGCCATTCCA GACCCTGTAG GTTTGAACAG AAACATGATG 
AACGAGGCGG ATGTGATGCA TCCCAATGTA CATCAAATCC ATTCAAGCGA GTCTGGCCTG 
AAGTCTGAAC TACCTAGGGT TTTAAAGTTG GATAAGTCAG TTGCCATCAG TGAGGCCGAT 
AAGTTGCAGC AAACATGCAA ATCACAGGGT ACTTCAGCAA GGAGTTGTAC GAAGGTCCAC 
AAGCAGGGGA TTGCACTTGG AAGGTCAGTT GATCTTAGCA GGTTCAACAA TTACGATGAA 
CTGGTTGCTG AATTGGACCA ACTGTTTGAA TTTGGTGGTG AGCTGCAGGC TCCCAAAAAG 
AATTGGCTTA TTGTATATAC GGATGATGAA GGTGACATGA TGCTTGTTGG AGACGATCCC 
TGGCGGGAGT TTTGTGGTAT GGTTCGGAAG ATTTTTATCT ATACAAGAGA AGAAGTCCAG 
AAGATGAATC CAGGATCCCT AAATTTGAAA GGCGATGAAA ATCCATCAGT TGAAGGGGAA 
GAGGTAAAAG AGACCAAAAG TCAGGCAGTT CCTTCGATGT CAGCACCAGA GAGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004138020.1
CDS	358..2877
Translation	MASSEVSINPNSASFNDHADSTKDTSDPPNALSPRDADIALYTELWNACAGPLVSVPRENERVFYFPQGHIEQVEASTSQVADQQMPVYNLPSKILCRVINVHLKAEPDTDEVFAQITLLPEANQDEHAVDKEPPPPPPRRFHVHSFCKTLTASDTSTHGGFSVLRRHADECLPPLDMSRQPPTQELVAKDLHGNEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQHGNVPSSVISSHSMHLGVLATAWHAISTGTLFTVYYKPRTSPSEFIVPYDQYMESIKKSYTIGMRFKMRFEGEEAPEQRFTGTIIGCEDADPKRWKDSKWRCLKVRWDETSTISRPEKVSPWKIEPALAPPALNPLPMTRPKRPRSNMVSTSPDSSVLTREGSSRVTVDPSPASAFTRVLQGQEFSTLRGNFIDGSDPDAAEKSVMWPPSLDDEKVDVVSTSKKHGADSWIPPGRSEPTYADLLSGFGTDMDSSHGVRAAMGDSALVTANSIRKHAMEHDGKFSFLGGSSWSVLPSGLSLNLVDSSQKGHIRAGDLSYQVRGNATFNGFGDHSISHCHRTEQPHGNWLMPPPSSHFDYPIHSSELMSKPMLFQNQDILKPKDGNCKLFGISLVKNPAIPDPVGLNRNMMNEADVMHPNVHQIHSSESGLKSELPRVLKLDKSVAISEADKLQQTCKSQGTSARSCTKVHKQGIALGRSVDLSRFNNYDELVAELDQLFEFGGELQAPKKNWLIVYTDDEGDMMLVGDDPWREFCGMVRKIFIYTREEVQKMNPGSLNLKGDENPSVEGEEVKETKSQAVPSMSAPESS

Target ORF information:

RefSeq Version	XM_004137972.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus auxin response factor 2 (LOC101206845), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004137972.2

ORF Insert Sequence:

ATGGCCTCCT CCGAGGTTTC GATCAACCCC AATTCCGCCT CCTTCAACGA CCATGCCGAT 
TCTACCAAAG ACACCTCCGA TCCACCCAAC GCTCTCTCTC CCAGGGATGC TGATATCGCT 
CTCTACACCG AGCTCTGGAA TGCTTGTGCT GGTCCTCTCG TTTCTGTTCC TCGTGAAAAC 
GAGCGCGTCT TCTACTTCCC TCAGGGCCAC ATCGAACAGG TGGAAGCATC AACTAGTCAA 
GTGGCGGACC AGCAGATGCC TGTTTATAAC CTTCCATCGA AGATCCTCTG CCGCGTTATT 
AATGTGCACT TAAAGGCCGA GCCAGACACC GATGAGGTAT TTGCACAGAT CACTTTGCTT 
CCAGAGGCTA ATCAAGATGA GCATGCTGTA GACAAGGAAC CTCCACCTCC TCCCCCGAGG 
AGATTTCACG TGCATTCTTT TTGCAAGACT CTGACTGCTT CTGACACTAG TACTCATGGG 
GGGTTTTCAG TCTTGAGACG CCATGCTGAT GAATGTCTTC CACCACTGGA CATGTCACGA 
CAGCCTCCTA CCCAGGAGTT GGTTGCAAAG GATTTGCATG GAAATGAATG GCGTTTCCGT 
CATATATTTC GAGGGCAACC ACGAAGGCAT TTGCTTCAGA GTGGTTGGAG TGTCTTTGTG 
AGCTCAAAAA GACTGGTTGC AGGAGATGCT TTTATATTTT TGAGGGGTGA GAATGGAGAG 
CTGCGTGTTG GTGTGAGGCG TGCAATGAGA CAACATGGCA ATGTTCCATC ATCGGTCATA 
TCTAGCCACA GCATGCATCT TGGTGTGCTT GCGACTGCTT GGCATGCTAT TTCGACAGGC 
ACATTGTTCA CTGTCTACTA TAAGCCTAGG ACAAGCCCTT CTGAATTCAT TGTTCCATAT 
GACCAGTATA TGGAGTCTAT CAAGAAGAGC TACACCATAG GAATGCGGTT TAAAATGAGA 
TTTGAAGGTG AAGAGGCTCC TGAGCAGAGG TTCACTGGTA CTATCATTGG ATGTGAAGAT 
GCTGATCCCA AAAGGTGGAA AGATTCCAAG TGGAGATGCC TGAAGGTGAG ATGGGATGAA 
ACTTCTACTA TTTCTCGTCC AGAGAAAGTC TCACCGTGGA AAATTGAGCC GGCCCTTGCT 
CCTCCTGCGC TGAATCCCCT TCCTATGACT AGACCTAAAA GGCCTCGATC AAACATGGTC 
TCTACATCCC CTGATTCCTC TGTTCTTACA CGAGAAGGCT CATCAAGAGT GACTGTAGAC 
CCTTCACCAG CCAGTGCGTT TACAAGGGTC TTGCAAGGTC AAGAATTCTC GACCTTGAGA 
GGTAATTTTA TCGATGGCAG TGATCCAGAT GCGGCTGAAA AATCTGTTAT GTGGCCTCCT 
TCATTAGATG ATGAGAAAGT TGACGTGGTT TCTACATCTA AGAAACATGG AGCAGATAGC 
TGGATACCTC CAGGAAGGAG TGAGCCAACC TACGCAGATC TACTGTCAGG TTTTGGAACT 
GATATGGATT CTTCTCATGG TGTTCGTGCT GCCATGGGAG ATTCTGCATT AGTCACTGCT 
AATTCAATCA GAAAGCATGC CATGGAACAC GATGGAAAAT TTAGCTTCCT TGGTGGTAGC 
TCATGGTCTG TCTTGCCTTC TGGTCTCTCG CTCAACCTGG TTGACTCCAG TCAGAAGGGT 
CATATTCGGG CTGGTGACTT ATCTTATCAA GTGAGAGGAA ATGCTACCTT CAATGGATTT 
GGCGATCATT CCATATCCCA TTGTCATAGA ACTGAGCAGC CTCATGGAAA TTGGCTGATG 
CCTCCACCTT CATCTCATTT TGACTATCCA ATTCATTCAA GTGAACTGAT GTCAAAACCT 
ATGTTATTTC AGAACCAAGA TATACTGAAA CCCAAAGATG GGAATTGTAA GCTTTTCGGT 
ATTTCCCTTG TTAAAAATCC TGCCATTCCA GACCCTGTAG GTTTGAACAG AAACATGATG 
AACGAGGCGG ATGTGATGCA TCCCAATGTA CATCAAATCC ATTCAAGCGA GTCTGGCCTG 
AAGTCTGAAC TACCTAGGGT TTTAAAGTTG GATAAGTCAG TTGCCATCAG TGAGGCCGAT 
AAGTTGCAGC AAACATGCAA ATCACAGGGT ACTTCAGCAA GGAGTTGTAC GAAGGTCCAC 
AAGCAGGGGA TTGCACTTGG AAGGTCAGTT GATCTTAGCA GGTTCAACAA TTACGATGAA 
CTGGTTGCTG AATTGGACCA ACTGTTTGAA TTTGGTGGTG AGCTGCAGGC TCCCAAAAAG 
AATTGGCTTA TTGTATATAC GGATGATGAA GGTGACATGA TGCTTGTTGG AGACGATCCC 
TGGCGGGAGT TTTGTGGTAT GGTTCGGAAG ATTTTTATCT ATACAAGAGA AGAAGTCCAG 
AAGATGAATC CAGGATCCCT AAATTTGAAA GGCGATGAAA ATCCATCAGT TGAAGGGGAA 
GAGGTAAAAG AGACCAAAAG TCAGGCAGTT CCTTCGATGT CAGCACCAGA GAGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101206845 ( XM_004137972.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004138020.1 Cucumis sativus auxin response factor 2A (LOC101206845), mRNA.

Price & Availability↑

CloneID	OCu52349
Clone ID Related Accession (Same CDS sequence)	XM_004137972.2 , XM_004137972.3
Accession Version	XM_004137972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	auxin response factor 2A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026655.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004137972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCT CCGAGGTTTC GATCAACCCC AATTCCGCCT CCTTCAACGA CCATGCCGAT 
TCTACCAAAG ACACCTCCGA TCCACCCAAC GCTCTCTCTC CCAGGGATGC TGATATCGCT 
CTCTACACCG AGCTCTGGAA TGCTTGTGCT GGTCCTCTCG TTTCTGTTCC TCGTGAAAAC 
GAGCGCGTCT TCTACTTCCC TCAGGGCCAC ATCGAACAGG TGGAAGCATC AACTAGTCAA 
GTGGCGGACC AGCAGATGCC TGTTTATAAC CTTCCATCGA AGATCCTCTG CCGCGTTATT 
AATGTGCACT TAAAGGCCGA GCCAGACACC GATGAGGTAT TTGCACAGAT CACTTTGCTT 
CCAGAGGCTA ATCAAGATGA GCATGCTGTA GACAAGGAAC CTCCACCTCC TCCCCCGAGG 
AGATTTCACG TGCATTCTTT TTGCAAGACT CTGACTGCTT CTGACACTAG TACTCATGGG 
GGGTTTTCAG TCTTGAGACG CCATGCTGAT GAATGTCTTC CACCACTGGA CATGTCACGA 
CAGCCTCCTA CCCAGGAGTT GGTTGCAAAG GATTTGCATG GAAATGAATG GCGTTTCCGT 
CATATATTTC GAGGGCAACC ACGAAGGCAT TTGCTTCAGA GTGGTTGGAG TGTCTTTGTG 
AGCTCAAAAA GACTGGTTGC AGGAGATGCT TTTATATTTT TGAGGGGTGA GAATGGAGAG 
CTGCGTGTTG GTGTGAGGCG TGCAATGAGA CAACATGGCA ATGTTCCATC ATCGGTCATA 
TCTAGCCACA GCATGCATCT TGGTGTGCTT GCGACTGCTT GGCATGCTAT TTCGACAGGC 
ACATTGTTCA CTGTCTACTA TAAGCCTAGG ACAAGCCCTT CTGAATTCAT TGTTCCATAT 
GACCAGTATA TGGAGTCTAT CAAGAAGAGC TACACCATAG GAATGCGGTT TAAAATGAGA 
TTTGAAGGTG AAGAGGCTCC TGAGCAGAGG TTCACTGGTA CTATCATTGG ATGTGAAGAT 
GCTGATCCCA AAAGGTGGAA AGATTCCAAG TGGAGATGCC TGAAGGTGAG ATGGGATGAA 
ACTTCTACTA TTTCTCGTCC AGAGAAAGTC TCACCGTGGA AAATTGAGCC GGCCCTTGCT 
CCTCCTGCGC TGAATCCCCT TCCTATGACT AGACCTAAAA GGCCTCGATC AAACATGGTC 
TCTACATCCC CTGATTCCTC TGTTCTTACA CGAGAAGGCT CATCAAGAGT GACTGTAGAC 
CCTTCACCAG CCAGTGCGTT TACAAGGGTC TTGCAAGGTC AAGAATTCTC GACCTTGAGA 
GGTAATTTTA TCGATGGCAG TGATCCAGAT GCGGCTGAAA AATCTGTTAT GTGGCCTCCT 
TCATTAGATG ATGAGAAAGT TGACGTGGTT TCTACATCTA AGAAACATGG AGCAGATAGC 
TGGATACCTC CAGGAAGGAG TGAGCCAACC TACGCAGATC TACTGTCAGG TTTTGGAACT 
GATATGGATT CTTCTCATGG TGTTCGTGCT GCCATGGGAG ATTCTGCATT AGTCACTGCT 
AATTCAATCA GAAAGCATGC CATGGAACAC GATGGAAAAT TTAGCTTCCT TGGTGGTAGC 
TCATGGTCTG TCTTGCCTTC TGGTCTCTCG CTCAACCTGG TTGACTCCAG TCAGAAGGGT 
CATATTCGGG CTGGTGACTT ATCTTATCAA GTGAGAGGAA ATGCTACCTT CAATGGATTT 
GGCGATCATT CCATATCCCA TTGTCATAGA ACTGAGCAGC CTCATGGAAA TTGGCTGATG 
CCTCCACCTT CATCTCATTT TGACTATCCA ATTCATTCAA GTGAACTGAT GTCAAAACCT 
ATGTTATTTC AGAACCAAGA TATACTGAAA CCCAAAGATG GGAATTGTAA GCTTTTCGGT 
ATTTCCCTTG TTAAAAATCC TGCCATTCCA GACCCTGTAG GTTTGAACAG AAACATGATG 
AACGAGGCGG ATGTGATGCA TCCCAATGTA CATCAAATCC ATTCAAGCGA GTCTGGCCTG 
AAGTCTGAAC TACCTAGGGT TTTAAAGTTG GATAAGTCAG TTGCCATCAG TGAGGCCGAT 
AAGTTGCAGC AAACATGCAA ATCACAGGGT ACTTCAGCAA GGAGTTGTAC GAAGGTCCAC 
AAGCAGGGGA TTGCACTTGG AAGGTCAGTT GATCTTAGCA GGTTCAACAA TTACGATGAA 
CTGGTTGCTG AATTGGACCA ACTGTTTGAA TTTGGTGGTG AGCTGCAGGC TCCCAAAAAG 
AATTGGCTTA TTGTATATAC GGATGATGAA GGTGACATGA TGCTTGTTGG AGACGATCCC 
TGGCGGGAGT TTTGTGGTAT GGTTCGGAAG ATTTTTATCT ATACAAGAGA AGAAGTCCAG 
AAGATGAATC CAGGATCCCT AAATTTGAAA GGCGATGAAA ATCCATCAGT TGAAGGGGAA 
GAGGTAAAAG AGACCAAAAG TCAGGCAGTT CCTTCGATGT CAGCACCAGA GAGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004138020.1
CDS	374..2893
Translation	MASSEVSINPNSASFNDHADSTKDTSDPPNALSPRDADIALYTELWNACAGPLVSVPRENERVFYFPQGHIEQVEASTSQVADQQMPVYNLPSKILCRVINVHLKAEPDTDEVFAQITLLPEANQDEHAVDKEPPPPPPRRFHVHSFCKTLTASDTSTHGGFSVLRRHADECLPPLDMSRQPPTQELVAKDLHGNEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQHGNVPSSVISSHSMHLGVLATAWHAISTGTLFTVYYKPRTSPSEFIVPYDQYMESIKKSYTIGMRFKMRFEGEEAPEQRFTGTIIGCEDADPKRWKDSKWRCLKVRWDETSTISRPEKVSPWKIEPALAPPALNPLPMTRPKRPRSNMVSTSPDSSVLTREGSSRVTVDPSPASAFTRVLQGQEFSTLRGNFIDGSDPDAAEKSVMWPPSLDDEKVDVVSTSKKHGADSWIPPGRSEPTYADLLSGFGTDMDSSHGVRAAMGDSALVTANSIRKHAMEHDGKFSFLGGSSWSVLPSGLSLNLVDSSQKGHIRAGDLSYQVRGNATFNGFGDHSISHCHRTEQPHGNWLMPPPSSHFDYPIHSSELMSKPMLFQNQDILKPKDGNCKLFGISLVKNPAIPDPVGLNRNMMNEADVMHPNVHQIHSSESGLKSELPRVLKLDKSVAISEADKLQQTCKSQGTSARSCTKVHKQGIALGRSVDLSRFNNYDELVAELDQLFEFGGELQAPKKNWLIVYTDDEGDMMLVGDDPWREFCGMVRKIFIYTREEVQKMNPGSLNLKGDENPSVEGEEVKETKSQAVPSMSAPESS

Target ORF information:

RefSeq Version	XM_004137972.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus auxin response factor 2A (LOC101206845), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004137972.3

ORF Insert Sequence:

ATGGCCTCCT CCGAGGTTTC GATCAACCCC AATTCCGCCT CCTTCAACGA CCATGCCGAT 
TCTACCAAAG ACACCTCCGA TCCACCCAAC GCTCTCTCTC CCAGGGATGC TGATATCGCT 
CTCTACACCG AGCTCTGGAA TGCTTGTGCT GGTCCTCTCG TTTCTGTTCC TCGTGAAAAC 
GAGCGCGTCT TCTACTTCCC TCAGGGCCAC ATCGAACAGG TGGAAGCATC AACTAGTCAA 
GTGGCGGACC AGCAGATGCC TGTTTATAAC CTTCCATCGA AGATCCTCTG CCGCGTTATT 
AATGTGCACT TAAAGGCCGA GCCAGACACC GATGAGGTAT TTGCACAGAT CACTTTGCTT 
CCAGAGGCTA ATCAAGATGA GCATGCTGTA GACAAGGAAC CTCCACCTCC TCCCCCGAGG 
AGATTTCACG TGCATTCTTT TTGCAAGACT CTGACTGCTT CTGACACTAG TACTCATGGG 
GGGTTTTCAG TCTTGAGACG CCATGCTGAT GAATGTCTTC CACCACTGGA CATGTCACGA 
CAGCCTCCTA CCCAGGAGTT GGTTGCAAAG GATTTGCATG GAAATGAATG GCGTTTCCGT 
CATATATTTC GAGGGCAACC ACGAAGGCAT TTGCTTCAGA GTGGTTGGAG TGTCTTTGTG 
AGCTCAAAAA GACTGGTTGC AGGAGATGCT TTTATATTTT TGAGGGGTGA GAATGGAGAG 
CTGCGTGTTG GTGTGAGGCG TGCAATGAGA CAACATGGCA ATGTTCCATC ATCGGTCATA 
TCTAGCCACA GCATGCATCT TGGTGTGCTT GCGACTGCTT GGCATGCTAT TTCGACAGGC 
ACATTGTTCA CTGTCTACTA TAAGCCTAGG ACAAGCCCTT CTGAATTCAT TGTTCCATAT 
GACCAGTATA TGGAGTCTAT CAAGAAGAGC TACACCATAG GAATGCGGTT TAAAATGAGA 
TTTGAAGGTG AAGAGGCTCC TGAGCAGAGG TTCACTGGTA CTATCATTGG ATGTGAAGAT 
GCTGATCCCA AAAGGTGGAA AGATTCCAAG TGGAGATGCC TGAAGGTGAG ATGGGATGAA 
ACTTCTACTA TTTCTCGTCC AGAGAAAGTC TCACCGTGGA AAATTGAGCC GGCCCTTGCT 
CCTCCTGCGC TGAATCCCCT TCCTATGACT AGACCTAAAA GGCCTCGATC AAACATGGTC 
TCTACATCCC CTGATTCCTC TGTTCTTACA CGAGAAGGCT CATCAAGAGT GACTGTAGAC 
CCTTCACCAG CCAGTGCGTT TACAAGGGTC TTGCAAGGTC AAGAATTCTC GACCTTGAGA 
GGTAATTTTA TCGATGGCAG TGATCCAGAT GCGGCTGAAA AATCTGTTAT GTGGCCTCCT 
TCATTAGATG ATGAGAAAGT TGACGTGGTT TCTACATCTA AGAAACATGG AGCAGATAGC 
TGGATACCTC CAGGAAGGAG TGAGCCAACC TACGCAGATC TACTGTCAGG TTTTGGAACT 
GATATGGATT CTTCTCATGG TGTTCGTGCT GCCATGGGAG ATTCTGCATT AGTCACTGCT 
AATTCAATCA GAAAGCATGC CATGGAACAC GATGGAAAAT TTAGCTTCCT TGGTGGTAGC 
TCATGGTCTG TCTTGCCTTC TGGTCTCTCG CTCAACCTGG TTGACTCCAG TCAGAAGGGT 
CATATTCGGG CTGGTGACTT ATCTTATCAA GTGAGAGGAA ATGCTACCTT CAATGGATTT 
GGCGATCATT CCATATCCCA TTGTCATAGA ACTGAGCAGC CTCATGGAAA TTGGCTGATG 
CCTCCACCTT CATCTCATTT TGACTATCCA ATTCATTCAA GTGAACTGAT GTCAAAACCT 
ATGTTATTTC AGAACCAAGA TATACTGAAA CCCAAAGATG GGAATTGTAA GCTTTTCGGT 
ATTTCCCTTG TTAAAAATCC TGCCATTCCA GACCCTGTAG GTTTGAACAG AAACATGATG 
AACGAGGCGG ATGTGATGCA TCCCAATGTA CATCAAATCC ATTCAAGCGA GTCTGGCCTG 
AAGTCTGAAC TACCTAGGGT TTTAAAGTTG GATAAGTCAG TTGCCATCAG TGAGGCCGAT 
AAGTTGCAGC AAACATGCAA ATCACAGGGT ACTTCAGCAA GGAGTTGTAC GAAGGTCCAC 
AAGCAGGGGA TTGCACTTGG AAGGTCAGTT GATCTTAGCA GGTTCAACAA TTACGATGAA 
CTGGTTGCTG AATTGGACCA ACTGTTTGAA TTTGGTGGTG AGCTGCAGGC TCCCAAAAAG 
AATTGGCTTA TTGTATATAC GGATGATGAA GGTGACATGA TGCTTGTTGG AGACGATCCC 
TGGCGGGAGT TTTGTGGTAT GGTTCGGAAG ATTTTTATCT ATACAAGAGA AGAAGTCCAG 
AAGATGAATC CAGGATCCCT AAATTTGAAA GGCGATGAAA ATCCATCAGT TGAAGGGGAA 
GAGGTAAAAG AGACCAAAAG TCAGGCAGTT CCTTCGATGT CAGCACCAGA GAGTTCTTAG