UBE4A cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	104068681
Full Name	ubiquitination factor E4A
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009571592.1	XP_009569887.1	ubiquitin conjugation factor E4 A isoform X1
XM_009571598.1	XP_009569893.1	ubiquitin conjugation factor E4 A isoform X2

Pathways from BioSystems
Gene Ontology

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu36919	XM_009571592.1 Latest version!	Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCu36920	XM_009571598.1 Latest version!	Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( XM_009571592.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009569887.1 Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu36919
Clone ID Related Accession (Same CDS sequence)	XM_009571592.1
Accession Version	XM_009571592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009243360.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGT 
TCCATCGCCG ACGCCAAGCA CTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
AGAGGCGACG AGGCTTCGGT CAGCCAAGAT GACTCGGACA ACAGCATCTC TGAGAGCCTG 
GATGACTGTG ATTACTCGGT GGCTGAGATC AACCGCTCCT TCCGCTCGCA GCGAGAGCTG 
TGCGAGCAGC TTAACATCAA CCACATGATC CAGAGGATCT TCCTTATCAC CCTCGACAAC 
AGCGATCCTA GCATGAAGAG TGGGAATGGG ATCCCAGTGC GCTGCGTCTA TCTGGAGGAA 
ATGGCCGCAG ACCTGGACGA GCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTG 
TTCACCCGCT TGCTGCTGCC GGAGCCTGCA AACCACCTGA TTTACATGAC CTCTGCCAGC 
ACACAGAACC TCTCTGCCGA CCGGGACGCC GGGGAGAGGC AGATCCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAAGTCC CAGAGAGCCT GCTGCCCTTT 
GCCGTGCGCT GCCGGAACCT GACGGTATCC AACACCCGCA CTGTCCTCCT CACCCCAGAG 
ATCTATGTCA ACCAAAATGT GCACGAGCAG CTGGTGGACC TGATGCTGGA GGCGCTGCAA 
GGGGCCCACT TTGAAGGTGT GACTGCATTT CTGGAGGAGG TGATAGAAGC CCTGACAATG 
GATGAGGAGG TGCGAACGTT TGGGGAGGTG ATGGTTCCAG TCTTTGATAT CCTGTCGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTTGA CGTGCTCCTC 
TACTTTACAA AGCAGAAGGA CATTGCCAAG GTTTTTGCAG GCTATATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACG TTGCTGGGTG CCATTTTGAA CATCTCCTGC 
TTGTTAAAGA CGCCCGGCGT CGTGGAGAAC CATGGCTATT TCCTAAATCC ATCCCGATCC 
AGCCCGCAGG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGGG CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG CTGCAGTTGT CTCCAGAGAC GAAACACAGG 
ATTCTTTCCT GGCTGGGAAA CTGCCTCTAC GCGAATGCAG GCCGCACCAA AATCTGGGCT 
AACCAGATGC CGGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTTAACCTG 
GGAGCTGCCC TCCTGAAGCT CTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTC 
ACCTTCAACC CCACCTACTG TGCCCTGAAG GAGCTGAACG AGGAGGAGAG GCGCAGCAAG 
AATGTACATA TGAAAGGTTT GGAAAAAGAA ACGTGCTTGA TACCTGCTCC AAGCGAGCAA 
GAGCCGGAGT TTGCGAACAG CTACAACCTG GTGACAGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTCCA CAGGTTGCAT GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTAGCATG GCGAGAAGCT CAGCAGAGCG CCAGCCCGGC TGCCGACAGC 
CTCCGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTCT CCACGAAGAC GGCAATGACA 
GAGCCGCAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TAAGCTTCCC GCTGCCGGAG 
GTGGAAAACA GCGCATTGGC CTATGTGCCA GAATTCTTTG CTGATAATCT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCCTGGAGA CTTCTGCTGA TTCTCTGGAG 
CATGTCCTTC ACTTCATTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCAGA AGTGCTGGAA GCGGTGATGC CGCACTTAGA CCAGGCCCAG 
AGCCCACTCG TCTCCAGTGT GTTTCATCGC AAGCGAGTGT TCTGCTCTTA CCGAAATGCT 
GCCCATCTTG CCGAGGCACT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGCGACCCA 
CACCAGTTTG AACAGAAGTT CAATTACCGC CGCCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACGG ACTCATACCG GCAGAGCATA AAGGCCCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTGAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTCCA GCAGATCGAG 
AAGGACCGCG GCGAGTGGGA CAGCCTGTCC CAGGAGGCTC GCCGTGAGAA GGAGTCAAGC 
CTGCAAATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATCGGC 
ACTCTGGCCT TCCTGACCTC TGAAATCAAG TCCCTGTTCG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACACCTGG TTGGTCCTAA AATGGGAGCT 
CTGAAAGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCGC AGCAGCTCGT GTCCGACATC 
TGCACCATCT ACCTGAACCT CGGGGATGAA GAAAACTTCT GTGCTACCGT GCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC ACTCTTCGCC CAGACCGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCCGGCA ACATGATCGT GTCTTTCAGT AACCTGGCTG AGAGAATTAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA GGAGACGTAC GCGGATGCCT GCGATGAGTT CCTGGATCCA 
ATCATGAGCA CTCTGATGTT GGACCCAGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGGTCAACCA TCGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTTAA CCGAAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTCAAAG AGAAGATCCA GCAGTGGCTC 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGAGACAC TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009569887.1
CDS	1..3228
Translation	MTDQENNNSISSNPFAALFGSIADAKHFAAVQKQQQLRQLRGDEASVSQDDSDNSISESLDDCDYSVAEINRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPVRCVYLEEMAADLDEQDWLDMDNVEQALFTRLLLPEPANHLIYMTSASTQNLSADRDAGERQILRYLYACFQRAREEITKVPESLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVHEQLVDLMLEALQGAHFEGVTAFLEEVIEALTMDEEVRTFGEVMVPVFDILSGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMGQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLYANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPAPSEQEPEFANSYNLVTENLVLTQYTLHLGFHRLHDQMVKINQSLHRLQVAWREAQQSASPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAMGNHGTEPLELSFPLPEVENSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHVLHFITVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQSPLVSSVFHRKRVFCSYRNAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRQSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSQEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMLDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQQWLAERKKQKEELEETLN

Target ORF information:

RefSeq Version	XM_009571592.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009571592.1

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGT 
TCCATCGCCG ACGCCAAGCA CTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
AGAGGCGACG AGGCTTCGGT CAGCCAAGAT GACTCGGACA ACAGCATCTC TGAGAGCCTG 
GATGACTGTG ATTACTCGGT GGCTGAGATC AACCGCTCCT TCCGCTCGCA GCGAGAGCTG 
TGCGAGCAGC TTAACATCAA CCACATGATC CAGAGGATCT TCCTTATCAC CCTCGACAAC 
AGCGATCCTA GCATGAAGAG TGGGAATGGG ATCCCAGTGC GCTGCGTCTA TCTGGAGGAA 
ATGGCCGCAG ACCTGGACGA GCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTG 
TTCACCCGCT TGCTGCTGCC GGAGCCTGCA AACCACCTGA TTTACATGAC CTCTGCCAGC 
ACACAGAACC TCTCTGCCGA CCGGGACGCC GGGGAGAGGC AGATCCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAAGTCC CAGAGAGCCT GCTGCCCTTT 
GCCGTGCGCT GCCGGAACCT GACGGTATCC AACACCCGCA CTGTCCTCCT CACCCCAGAG 
ATCTATGTCA ACCAAAATGT GCACGAGCAG CTGGTGGACC TGATGCTGGA GGCGCTGCAA 
GGGGCCCACT TTGAAGGTGT GACTGCATTT CTGGAGGAGG TGATAGAAGC CCTGACAATG 
GATGAGGAGG TGCGAACGTT TGGGGAGGTG ATGGTTCCAG TCTTTGATAT CCTGTCGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTTGA CGTGCTCCTC 
TACTTTACAA AGCAGAAGGA CATTGCCAAG GTTTTTGCAG GCTATATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACG TTGCTGGGTG CCATTTTGAA CATCTCCTGC 
TTGTTAAAGA CGCCCGGCGT CGTGGAGAAC CATGGCTATT TCCTAAATCC ATCCCGATCC 
AGCCCGCAGG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGGG CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG CTGCAGTTGT CTCCAGAGAC GAAACACAGG 
ATTCTTTCCT GGCTGGGAAA CTGCCTCTAC GCGAATGCAG GCCGCACCAA AATCTGGGCT 
AACCAGATGC CGGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTTAACCTG 
GGAGCTGCCC TCCTGAAGCT CTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTC 
ACCTTCAACC CCACCTACTG TGCCCTGAAG GAGCTGAACG AGGAGGAGAG GCGCAGCAAG 
AATGTACATA TGAAAGGTTT GGAAAAAGAA ACGTGCTTGA TACCTGCTCC AAGCGAGCAA 
GAGCCGGAGT TTGCGAACAG CTACAACCTG GTGACAGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTCCA CAGGTTGCAT GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTAGCATG GCGAGAAGCT CAGCAGAGCG CCAGCCCGGC TGCCGACAGC 
CTCCGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTCT CCACGAAGAC GGCAATGACA 
GAGCCGCAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TAAGCTTCCC GCTGCCGGAG 
GTGGAAAACA GCGCATTGGC CTATGTGCCA GAATTCTTTG CTGATAATCT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCCTGGAGA CTTCTGCTGA TTCTCTGGAG 
CATGTCCTTC ACTTCATTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCAGA AGTGCTGGAA GCGGTGATGC CGCACTTAGA CCAGGCCCAG 
AGCCCACTCG TCTCCAGTGT GTTTCATCGC AAGCGAGTGT TCTGCTCTTA CCGAAATGCT 
GCCCATCTTG CCGAGGCACT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGCGACCCA 
CACCAGTTTG AACAGAAGTT CAATTACCGC CGCCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACGG ACTCATACCG GCAGAGCATA AAGGCCCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTGAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTCCA GCAGATCGAG 
AAGGACCGCG GCGAGTGGGA CAGCCTGTCC CAGGAGGCTC GCCGTGAGAA GGAGTCAAGC 
CTGCAAATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATCGGC 
ACTCTGGCCT TCCTGACCTC TGAAATCAAG TCCCTGTTCG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACACCTGG TTGGTCCTAA AATGGGAGCT 
CTGAAAGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCGC AGCAGCTCGT GTCCGACATC 
TGCACCATCT ACCTGAACCT CGGGGATGAA GAAAACTTCT GTGCTACCGT GCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC ACTCTTCGCC CAGACCGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCCGGCA ACATGATCGT GTCTTTCAGT AACCTGGCTG AGAGAATTAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA GGAGACGTAC GCGGATGCCT GCGATGAGTT CCTGGATCCA 
ATCATGAGCA CTCTGATGTT GGACCCAGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGGTCAACCA TCGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTTAA CCGAAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTCAAAG AGAAGATCCA GCAGTGGCTC 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGAGACAC TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( XM_009571598.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009569893.1 Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCu36920
Clone ID Related Accession (Same CDS sequence)	XM_009571598.1
Accession Version	XM_009571598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009243360.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGT 
TCCATCGCCG ACGCCAAGCA CTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
AGAGGTGAGG GAGCTTCGGT CAGCCAAGAT GACTCGGACA ACAGCATCTC TGAGAGCCTG 
GATGACTGTG ATTACTCGGT GGCTGAGATC AACCGCTCCT TCCGCTCGCA GCGAGAGCTG 
TGCGAGCAGC TTAACATCAA CCACATGATC CAGAGGATCT TCCTTATCAC CCTCGACAAC 
AGCGATCCTA GCATGAAGAG TGGGAATGGG ATCCCAGTGC GCTGCGTCTA TCTGGAGGAA 
ATGGCCGCAG ACCTGGACGA GCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTG 
TTCACCCGCT TGCTGCTGCC GGAGCCTGCA AACCACCTGA TTTACATGAC CTCTGCCAGC 
ACACAGAACC TCTCTGCCGA CCGGGACGCC GGGGAGAGGC AGATCCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAAGTCC CAGAGAGCCT GCTGCCCTTT 
GCCGTGCGCT GCCGGAACCT GACGGTATCC AACACCCGCA CTGTCCTCCT CACCCCAGAG 
ATCTATGTCA ACCAAAATGT GCACGAGCAG CTGGTGGACC TGATGCTGGA GGCGCTGCAA 
GGGGCCCACT TTGAAGGTGT GACTGCATTT CTGGAGGAGG TGATAGAAGC CCTGACAATG 
GATGAGGAGG TGCGAACGTT TGGGGAGGTG ATGGTTCCAG TCTTTGATAT CCTGTCGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTTGA CGTGCTCCTC 
TACTTTACAA AGCAGAAGGA CATTGCCAAG GTTTTTGCAG GCTATATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACG TTGCTGGGTG CCATTTTGAA CATCTCCTGC 
TTGTTAAAGA CGCCCGGCGT CGTGGAGAAC CATGGCTATT TCCTAAATCC ATCCCGATCC 
AGCCCGCAGG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGGG CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG CTGCAGTTGT CTCCAGAGAC GAAACACAGG 
ATTCTTTCCT GGCTGGGAAA CTGCCTCTAC GCGAATGCAG GCCGCACCAA AATCTGGGCT 
AACCAGATGC CGGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTTAACCTG 
GGAGCTGCCC TCCTGAAGCT CTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTC 
ACCTTCAACC CCACCTACTG TGCCCTGAAG GAGCTGAACG AGGAGGAGAG GCGCAGCAAG 
AATGTACATA TGAAAGGTTT GGAAAAAGAA ACGTGCTTGA TACCTGCTCC AAGCGAGCAA 
GAGCCGGAGT TTGCGAACAG CTACAACCTG GTGACAGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTCCA CAGGTTGCAT GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTAGCATG GCGAGAAGCT CAGCAGAGCG CCAGCCCGGC TGCCGACAGC 
CTCCGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTCT CCACGAAGAC GGCAATGACA 
GAGCCGCAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TAAGCTTCCC GCTGCCGGAG 
GTGGAAAACA GCGCATTGGC CTATGTGCCA GAATTCTTTG CTGATAATCT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCCTGGAGA CTTCTGCTGA TTCTCTGGAG 
CATGTCCTTC ACTTCATTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCAGA AGTGCTGGAA GCGGTGATGC CGCACTTAGA CCAGGCCCAG 
AGCCCACTCG TCTCCAGTGT GTTTCATCGC AAGCGAGTGT TCTGCTCTTA CCGAAATGCT 
GCCCATCTTG CCGAGGCACT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGCGACCCA 
CACCAGTTTG AACAGAAGTT CAATTACCGC CGCCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACGG ACTCATACCG GCAGAGCATA AAGGCCCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTGAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTCCA GCAGATCGAG 
AAGGACCGCG GCGAGTGGGA CAGCCTGTCC CAGGAGGCTC GCCGTGAGAA GGAGTCAAGC 
CTGCAAATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATCGGC 
ACTCTGGCCT TCCTGACCTC TGAAATCAAG TCCCTGTTCG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACACCTGG TTGGTCCTAA AATGGGAGCT 
CTGAAAGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCGC AGCAGCTCGT GTCCGACATC 
TGCACCATCT ACCTGAACCT CGGGGATGAA GAAAACTTCT GTGCTACCGT GCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC ACTCTTCGCC CAGACCGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCCGGCA ACATGATCGT GTCTTTCAGT AACCTGGCTG AGAGAATTAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA GGAGACGTAC GCGGATGCCT GCGATGAGTT CCTGGATCCA 
ATCATGAGCA CTCTGATGTT GGACCCAGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGGTCAACCA TCGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTTAA CCGAAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTCAAAG AGAAGATCCA GCAGTGGCTC 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGAGACAC TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009569893.1
CDS	41..3268
Translation	MTDQENNNSISSNPFAALFGSIADAKHFAAVQKQQQLRQLRGEGASVSQDDSDNSISESLDDCDYSVAEINRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPVRCVYLEEMAADLDEQDWLDMDNVEQALFTRLLLPEPANHLIYMTSASTQNLSADRDAGERQILRYLYACFQRAREEITKVPESLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVHEQLVDLMLEALQGAHFEGVTAFLEEVIEALTMDEEVRTFGEVMVPVFDILSGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMGQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLYANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPAPSEQEPEFANSYNLVTENLVLTQYTLHLGFHRLHDQMVKINQSLHRLQVAWREAQQSASPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAMGNHGTEPLELSFPLPEVENSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHVLHFITVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQSPLVSSVFHRKRVFCSYRNAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRQSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSQEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMLDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQQWLAERKKQKEELEETLN

Target ORF information:

RefSeq Version	XM_009571598.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009571598.1

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGT 
TCCATCGCCG ACGCCAAGCA CTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
AGAGGTGAGG GAGCTTCGGT CAGCCAAGAT GACTCGGACA ACAGCATCTC TGAGAGCCTG 
GATGACTGTG ATTACTCGGT GGCTGAGATC AACCGCTCCT TCCGCTCGCA GCGAGAGCTG 
TGCGAGCAGC TTAACATCAA CCACATGATC CAGAGGATCT TCCTTATCAC CCTCGACAAC 
AGCGATCCTA GCATGAAGAG TGGGAATGGG ATCCCAGTGC GCTGCGTCTA TCTGGAGGAA 
ATGGCCGCAG ACCTGGACGA GCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTG 
TTCACCCGCT TGCTGCTGCC GGAGCCTGCA AACCACCTGA TTTACATGAC CTCTGCCAGC 
ACACAGAACC TCTCTGCCGA CCGGGACGCC GGGGAGAGGC AGATCCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAAGTCC CAGAGAGCCT GCTGCCCTTT 
GCCGTGCGCT GCCGGAACCT GACGGTATCC AACACCCGCA CTGTCCTCCT CACCCCAGAG 
ATCTATGTCA ACCAAAATGT GCACGAGCAG CTGGTGGACC TGATGCTGGA GGCGCTGCAA 
GGGGCCCACT TTGAAGGTGT GACTGCATTT CTGGAGGAGG TGATAGAAGC CCTGACAATG 
GATGAGGAGG TGCGAACGTT TGGGGAGGTG ATGGTTCCAG TCTTTGATAT CCTGTCGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTTGA CGTGCTCCTC 
TACTTTACAA AGCAGAAGGA CATTGCCAAG GTTTTTGCAG GCTATATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACG TTGCTGGGTG CCATTTTGAA CATCTCCTGC 
TTGTTAAAGA CGCCCGGCGT CGTGGAGAAC CATGGCTATT TCCTAAATCC ATCCCGATCC 
AGCCCGCAGG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGGG CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG CTGCAGTTGT CTCCAGAGAC GAAACACAGG 
ATTCTTTCCT GGCTGGGAAA CTGCCTCTAC GCGAATGCAG GCCGCACCAA AATCTGGGCT 
AACCAGATGC CGGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTTAACCTG 
GGAGCTGCCC TCCTGAAGCT CTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTC 
ACCTTCAACC CCACCTACTG TGCCCTGAAG GAGCTGAACG AGGAGGAGAG GCGCAGCAAG 
AATGTACATA TGAAAGGTTT GGAAAAAGAA ACGTGCTTGA TACCTGCTCC AAGCGAGCAA 
GAGCCGGAGT TTGCGAACAG CTACAACCTG GTGACAGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTCCA CAGGTTGCAT GACCAGATGG TAAAGATAAA CCAAAGCCTT 
CACCGCCTGC AAGTAGCATG GCGAGAAGCT CAGCAGAGCG CCAGCCCGGC TGCCGACAGC 
CTCCGGGAGC AGTTTGAGCG CCTGATGACC ATCTATCTCT CCACGAAGAC GGCAATGACA 
GAGCCGCAGA TGCTGCAGAA CTGCCTGAAC CTGCAGGTGT CCATGGCAGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TAAGCTTCCC GCTGCCGGAG 
GTGGAAAACA GCGCATTGGC CTATGTGCCA GAATTCTTTG CTGATAATCT GGGCGACTTC 
TTCATTTTCC TGCGGCGTTT TGCTGATGAC ATCCTGGAGA CTTCTGCTGA TTCTCTGGAG 
CATGTCCTTC ACTTCATTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAC 
CTGCGAGCCA AGCTGGCAGA AGTGCTGGAA GCGGTGATGC CGCACTTAGA CCAGGCCCAG 
AGCCCACTCG TCTCCAGTGT GTTTCATCGC AAGCGAGTGT TCTGCTCTTA CCGAAATGCT 
GCCCATCTTG CCGAGGCACT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGCGACCCA 
CACCAGTTTG AACAGAAGTT CAATTACCGC CGCCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACGG ACTCATACCG GCAGAGCATA AAGGCCCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTGAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGCAAGA TCAAAGTCCA GCAGATCGAG 
AAGGACCGCG GCGAGTGGGA CAGCCTGTCC CAGGAGGCTC GCCGTGAGAA GGAGTCAAGC 
CTGCAAATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATCGGC 
ACTCTGGCCT TCCTGACCTC TGAAATCAAG TCCCTGTTCG TGCATCCTTT CCTTGCCGAG 
CGCATCATCT CCATGCTCAA CTACTTCCTG CAACACCTGG TTGGTCCTAA AATGGGAGCT 
CTGAAAGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCGC AGCAGCTCGT GTCCGACATC 
TGCACCATCT ACCTGAACCT CGGGGATGAA GAAAACTTCT GTGCTACCGT GCCCAAGGAC 
GGCCGCTCCT ACTCACCGAC ACTCTTCGCC CAGACCGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCCGGCA ACATGATCGT GTCTTTCAGT AACCTGGCTG AGAGAATTAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA GGAGACGTAC GCGGATGCCT GCGATGAGTT CCTGGATCCA 
ATCATGAGCA CTCTGATGTT GGACCCAGTG ATCCTGCCCT CCTCCCGTGT CACTGTGGAC 
CGGTCAACCA TCGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTTAA CCGAAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTCAAAG AGAAGATCCA GCAGTGGCTC 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGAGACAC TGAACTGA