NEDD4 cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEDD4
Entrez Gene ID	109308991
Full Name	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Crocodylus porosus(Australian saltwater crocodile)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019533527.1	XP_019389072.1	E3 ubiquitin-protein ligase NEDD4 isoform X2
XM_019533526.1	XP_019389071.1	E3 ubiquitin-protein ligase NEDD4 isoform X1

Pathways from BioSystems
Gene Ontology

The following NEDD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr68223	XM_019533527.1 Latest version!	Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCr68222	XM_019533526.1 Latest version!	Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEDD4 ( XM_019533527.1 ) cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile) -> XP_019389072.1 Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr68223
Clone ID Related Accession (Same CDS sequence)	XM_019533527.1
Accession Version	XM_019533527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-19
Organism	Crocodylus porosus(Australian saltwater crocodile)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728911.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crocodylus porosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTCCCGTGGA GTCGCTGATG CTGGGGCTGG CGGGGGACGA GGAAAATACA 
CGAATTGTGA GGGTGAAGAT TATAGCTGGA ATTAGTCTTG CCAAAAAGGA TATCTTGGGA 
GCAAGTGATC CTTATGTGAA AGTGACAGTA TATGATCCAG TGGATGGAAT ACTTACCAGT 
GTTCAAACAA AAACTATTAA AAAGACCTTG AATCCAAAGT GGAATGAGGA GCTTTTATTC 
AGAGTTAATC CTCAGAAACA CCGGCTTCTT TTTGAAGTGT TTGATGAAAA CCGTCTGACC 
AGAGATGACT TCCTAGGCCA AGTGGACGTT CCTCTTTATC AGTTACCGAC AGAAAATCCA 
AGTATGGAGA GACCATATAC ATTTAAGGAT TTTGTTCTTC ATCCAAGAAG TCACAAATCA 
AAAGTTAAAG GTCATCTTAG ATTAAAAATG ACTTACTTAC CTAAAAATAA TGGATCTGAA 
GAAGAAAATA CAGAACAGGC AGAGGAATTA GAGCCTGGCT GGATTATATT GGACCAACCA 
GATGCTGCTT GCCAGCTGCA ACACCAGCAA CAGGAAATTC CTCCCCTACC TCCAGGCTGG 
GAAGAAAGGC AAGATATACT TGGCAGGACT TATTATGTCA ACCATGAATT CAGAAGGACA 
CAGTGGAAGA GACCAACTGC TCAGGACAGT ACAGCTGATG CAGATCATGG GAACTTTCAG 
TTGGAAGCAC AGCATGCATT TACTCATCGG CATCAAATAT CAGAAGATTC TGAAAATTTA 
GACAACAGGG ATTCTCCTGA GAGCTGGGAA ATCATAACTG AAGATGAGGC AACTATGTAT 
AGCAATCAGA GTCTACAAAT GCCTCTGTCA CAGAGTAATT CTGATGTACA GACTCAGCTT 
GCAGAAGAAT TGAATACCAG ACTTACTACC TCCGGGAGTT CAGCCAGTGG CCAACCAGCT 
CTTTACACTA ATCATTCCAG CAGAAGAAGT AGTCTGCAGA CGTACAGGGT TGAGGAACAG 
CCTGCACTTC CTGTGTTATT GCCTACTTCA TCTGGATTGC CTCCAGGTTG GGAAGAAAGA 
CAAGATGAAA AAGGACGATC ATATTACATA GATCACAATT CCAGAACTAC TACCTGGATA 
AAGCCTGTTG TTCAGACTAC AGTAGAAACG GGTCAGCTAT CAACTGTCCA GAGTACTTCT 
ACAGGACAGC AGCCACCATC AAGTGATTCA TCACAATCTG AAACAGATCA AGGATTACTC 
CCTAAAGGCT GGGAAGTCCG ACATGCACCA AATGGAAGAC CATTCTTTAT TGATCACAAT 
ACCAAAACTA CTACATGGGA AGATCCAAGA TTGAAAATTC CAGCTCATCT AAGAAGAAAG 
ACCTCTCTAG ATCCCACAGA TCTAGGCCCC TTGCCACCAG GATGGGAAGA GAGAACTCAT 
ACAGATGGAA GAATATTCTA CATAAACCAC AACACAAAGA AAACACAATG GGAAGATCCA 
CGACTACAGA CTGCAGCGAT TACTGGACCA GCTGTGCCTT ACTCTAGGGA TTACAAAAGA 
AAATATGAAT TCTTCAGAAA AAAGTTGAAG AAGCAGAGTG ATATTCCAAA TAGATTTGAA 
ATGAAAGTTC ATCGCACAAC AATCCTTGAA GATTCTTATA GAAGAATTAT AGCTGTAAAA 
AGAGCAGAAT TCCTCAAAGC AAAACTCTGG ATTGAGTTTG ATGGTGAAAA AGGATTAGAT 
TATGGAGGAG TGGCAAGGGA ATGGTTCTTC CTTCTCTCTA AAGAAATGTT TAATCCCTAT 
TATGGATTGT TTGAATATTC TGCTACGGAT AACTATACTC TGCAGATCAA TCCAAATTCT 
GGCTTGTGCA ACGAAGATCA TCTCTCTTAC TTCAAATTTA TAGGTCGTGT AGCTGGAATG 
GCTGTTTACC ATGGCAAGCT TTTGGATGCC TTTTTTATTC GGCCTTTTTA CAAGATGATG 
CTTCAAAAAC CAATAACGCT TCACGACATG GAATCTGTGG ACAGTGAATA TTACAATTCT 
TTGAAATGGA TTCTTGAAAA TGACCCAGCA GAATTGGACC TCAGGTTTAT GGTTGATGAA 
GAGCTATTTG GACAGACTCA TCAACATGAT TTGAAAAGTG GTGGATCAGA AATAGTAGTT 
ACCAACAAGA ACAAACGAGA CTATATTCAC CTTGTAATTC AATGGAGGTT TGTGAGCAGA 
GTCCAGAAAC AAATGGCTGC CTTTAAGGAG GGATTTTTTG AACTGATACC ACAGGACCTA 
ATTAAAATTT TTGATGAAAA TGAATTAGAG TTATTAATGT GTGGACTAGG AGATGTGGAT 
GTGGCTGACT GGAAACTACA TACAAAATAC AAAAATGGTT ACAGCATAAA TCACCAAGTT 
ATACAGTGGT TTTGGAAGGC AGTTTTGATG ATGGATTCCG AAAAGCGAAT ACGATTACTT 
CAGTTTGTCA CTGGTACATC CCGTGTACCT ATGAATGGAT TTGCTGAATT ATATGGTTCA 
AATGGACCAC AGTTGTTTAC AATCGAACAG TGGGGCACCC CGGAAAAACT GCCAAGAGCT 
CATACTTGCT TTAATCGCTT GGATTTGCCT CCATATGACT CATTTGAAGA CTTATGGGAA 
AAACTCCTCT TAGCAATTGA AAATACTCAG GGTTTTGATG GAGTCGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019389072.1
CDS	53..2743
Translation	MAASPVESLMLGLAGDEENTRIVRVKIIAGISLAKKDILGASDPYVKVTVYDPVDGILTSVQTKTIKKTLNPKWNEELLFRVNPQKHRLLFEVFDENRLTRDDFLGQVDVPLYQLPTENPSMERPYTFKDFVLHPRSHKSKVKGHLRLKMTYLPKNNGSEEENTEQAEELEPGWIILDQPDAACQLQHQQQEIPPLPPGWEERQDILGRTYYVNHEFRRTQWKRPTAQDSTADADHGNFQLEAQHAFTHRHQISEDSENLDNRDSPESWEIITEDEATMYSNQSLQMPLSQSNSDVQTQLAEELNTRLTTSGSSASGQPALYTNHSSRRSSLQTYRVEEQPALPVLLPTSSGLPPGWEERQDEKGRSYYIDHNSRTTTWIKPVVQTTVETGQLSTVQSTSTGQQPPSSDSSQSETDQGLLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRRKTSLDPTDLGPLPPGWEERTHTDGRIFYINHNTKKTQWEDPRLQTAAITGPAVPYSRDYKRKYEFFRKKLKKQSDIPNRFEMKVHRTTILEDSYRRIIAVKRAEFLKAKLWIEFDGEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLHDMESVDSEYYNSLKWILENDPAELDLRFMVDEELFGQTHQHDLKSGGSEIVVTNKNKRDYIHLVIQWRFVSRVQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVADWKLHTKYKNGYSINHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPEKLPRAHTCFNRLDLPPYDSFEDLWEKLLLAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_019533527.1
Organism	Crocodylus porosus(Australian saltwater crocodile)
Definition	Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019533527.1

ORF Insert Sequence:

ATGGCGGCCT CTCCCGTGGA GTCGCTGATG CTGGGGCTGG CGGGGGACGA GGAAAATACA 
CGAATTGTGA GGGTGAAGAT TATAGCTGGA ATTAGTCTTG CCAAAAAGGA TATCTTGGGA 
GCAAGTGATC CTTATGTGAA AGTGACAGTA TATGATCCAG TGGATGGAAT ACTTACCAGT 
GTTCAAACAA AAACTATTAA AAAGACCTTG AATCCAAAGT GGAATGAGGA GCTTTTATTC 
AGAGTTAATC CTCAGAAACA CCGGCTTCTT TTTGAAGTGT TTGATGAAAA CCGTCTGACC 
AGAGATGACT TCCTAGGCCA AGTGGACGTT CCTCTTTATC AGTTACCGAC AGAAAATCCA 
AGTATGGAGA GACCATATAC ATTTAAGGAT TTTGTTCTTC ATCCAAGAAG TCACAAATCA 
AAAGTTAAAG GTCATCTTAG ATTAAAAATG ACTTACTTAC CTAAAAATAA TGGATCTGAA 
GAAGAAAATA CAGAACAGGC AGAGGAATTA GAGCCTGGCT GGATTATATT GGACCAACCA 
GATGCTGCTT GCCAGCTGCA ACACCAGCAA CAGGAAATTC CTCCCCTACC TCCAGGCTGG 
GAAGAAAGGC AAGATATACT TGGCAGGACT TATTATGTCA ACCATGAATT CAGAAGGACA 
CAGTGGAAGA GACCAACTGC TCAGGACAGT ACAGCTGATG CAGATCATGG GAACTTTCAG 
TTGGAAGCAC AGCATGCATT TACTCATCGG CATCAAATAT CAGAAGATTC TGAAAATTTA 
GACAACAGGG ATTCTCCTGA GAGCTGGGAA ATCATAACTG AAGATGAGGC AACTATGTAT 
AGCAATCAGA GTCTACAAAT GCCTCTGTCA CAGAGTAATT CTGATGTACA GACTCAGCTT 
GCAGAAGAAT TGAATACCAG ACTTACTACC TCCGGGAGTT CAGCCAGTGG CCAACCAGCT 
CTTTACACTA ATCATTCCAG CAGAAGAAGT AGTCTGCAGA CGTACAGGGT TGAGGAACAG 
CCTGCACTTC CTGTGTTATT GCCTACTTCA TCTGGATTGC CTCCAGGTTG GGAAGAAAGA 
CAAGATGAAA AAGGACGATC ATATTACATA GATCACAATT CCAGAACTAC TACCTGGATA 
AAGCCTGTTG TTCAGACTAC AGTAGAAACG GGTCAGCTAT CAACTGTCCA GAGTACTTCT 
ACAGGACAGC AGCCACCATC AAGTGATTCA TCACAATCTG AAACAGATCA AGGATTACTC 
CCTAAAGGCT GGGAAGTCCG ACATGCACCA AATGGAAGAC CATTCTTTAT TGATCACAAT 
ACCAAAACTA CTACATGGGA AGATCCAAGA TTGAAAATTC CAGCTCATCT AAGAAGAAAG 
ACCTCTCTAG ATCCCACAGA TCTAGGCCCC TTGCCACCAG GATGGGAAGA GAGAACTCAT 
ACAGATGGAA GAATATTCTA CATAAACCAC AACACAAAGA AAACACAATG GGAAGATCCA 
CGACTACAGA CTGCAGCGAT TACTGGACCA GCTGTGCCTT ACTCTAGGGA TTACAAAAGA 
AAATATGAAT TCTTCAGAAA AAAGTTGAAG AAGCAGAGTG ATATTCCAAA TAGATTTGAA 
ATGAAAGTTC ATCGCACAAC AATCCTTGAA GATTCTTATA GAAGAATTAT AGCTGTAAAA 
AGAGCAGAAT TCCTCAAAGC AAAACTCTGG ATTGAGTTTG ATGGTGAAAA AGGATTAGAT 
TATGGAGGAG TGGCAAGGGA ATGGTTCTTC CTTCTCTCTA AAGAAATGTT TAATCCCTAT 
TATGGATTGT TTGAATATTC TGCTACGGAT AACTATACTC TGCAGATCAA TCCAAATTCT 
GGCTTGTGCA ACGAAGATCA TCTCTCTTAC TTCAAATTTA TAGGTCGTGT AGCTGGAATG 
GCTGTTTACC ATGGCAAGCT TTTGGATGCC TTTTTTATTC GGCCTTTTTA CAAGATGATG 
CTTCAAAAAC CAATAACGCT TCACGACATG GAATCTGTGG ACAGTGAATA TTACAATTCT 
TTGAAATGGA TTCTTGAAAA TGACCCAGCA GAATTGGACC TCAGGTTTAT GGTTGATGAA 
GAGCTATTTG GACAGACTCA TCAACATGAT TTGAAAAGTG GTGGATCAGA AATAGTAGTT 
ACCAACAAGA ACAAACGAGA CTATATTCAC CTTGTAATTC AATGGAGGTT TGTGAGCAGA 
GTCCAGAAAC AAATGGCTGC CTTTAAGGAG GGATTTTTTG AACTGATACC ACAGGACCTA 
ATTAAAATTT TTGATGAAAA TGAATTAGAG TTATTAATGT GTGGACTAGG AGATGTGGAT 
GTGGCTGACT GGAAACTACA TACAAAATAC AAAAATGGTT ACAGCATAAA TCACCAAGTT 
ATACAGTGGT TTTGGAAGGC AGTTTTGATG ATGGATTCCG AAAAGCGAAT ACGATTACTT 
CAGTTTGTCA CTGGTACATC CCGTGTACCT ATGAATGGAT TTGCTGAATT ATATGGTTCA 
AATGGACCAC AGTTGTTTAC AATCGAACAG TGGGGCACCC CGGAAAAACT GCCAAGAGCT 
CATACTTGCT TTAATCGCTT GGATTTGCCT CCATATGACT CATTTGAAGA CTTATGGGAA 
AAACTCCTCT TAGCAATTGA AAATACTCAG GGTTTTGATG GAGTCGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_019533526.1 ) cDNA ORF clone, Crocodylus porosus(Australian saltwater crocodile) -> XP_019389071.1 Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr68222
Clone ID Related Accession (Same CDS sequence)	XM_019533526.1
Accession Version	XM_019533526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-19
Organism	Crocodylus porosus(Australian saltwater crocodile)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728911.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crocodylus porosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGAA GGTTAAGATT GCATTTTGCA GCCCGAAGAA GCAACACCGA TCCACTGTCA 
GAAGCCTCTG AAGATGACTT GGATAATAAT ATTTATATGT ATTTCAAAAG ACCTTCTCAG 
AATTCTGCAT CTAATGTCAT AGAAATGAAA CTGACTCCCA GACGAGCAGC CTTTCCACCT 
GTAATGCAGG AAATAATTAA TCAACAACAA AGTGCAAGTG GTTCTTCGCT GCAAAAAAAC 
AACAAAAGGA GCACTTCACT TCAGATTTCC CTGCAGTCTA GAAAGTACAG TGGATGTCTT 
CAGTCTGGTG ATATAGTAGC TACTAATGAA GATTCATCAT TCACTGGTGT TGGCAATGAA 
GGGAACTGTT CTGGGAGAAA TATTGGTGAT TATCCGTCTA ACCCGATAAA TACCACCAGC 
CACTTGAGCA GTACTACCAT GTTCAATGAC AGTCTTGGCA GTATTGCAGC CAGTGACAAT 
GGATCCTTCA GTAGTGCTGG CAGTGACTAT GGATCATGCA CAAGTACCAC AAGTGATGGA 
GGTTCACACT GCAACAGTGT CCTTAGTGAC AATAGTAGTT GCACTCTTTC CACAGGTGAT 
GGTACTTTTT ATAATAGCAT TGCATATGAT AACTCTTCGT ATAGCAGAGC TACAAACAAA 
TATAATTCCT ATTGGAAAGA TGCATCTCAG GAAAGTTTGT GTCAAAGTAA TACTATTTTT 
GACCAAGATG GTTTGGAGCA AGTGAAATTG TCAGCGAGGA GGAAAACTAA AGTTCCACTA 
AGACGTTGTT CATCGTTGGT AATTTTCCCT CGAAGTCCTT CCAGTACTCC ACCAATGTCT 
CCAACAAGCT CAGGTCCTCC ACCACAGAGT GTGGCATATC AGACCTCACA TAAACTAGTT 
ATTTCTCCAG GTGATCTGGC ACAACATGAA AATCAAACTG GTAATAAGGA GTTCCTTTCA 
ACAGCAGTCA ATGGACTTCG ACTCTCTAAA TCTGCTTTCT CTCCTGGTGA GGTTAGAGCG 
ATTAGACCAC TTTACTTGAA TAGTTCATTA CAGGCCAAAA GTCAAGCCAC AGATAGTTCT 
GAGCAGGCAA CTTGTGAAGA AACATCTGAT GGCTTTTCTT GTATTTCAGT TCACCGTCCT 
CAAAAAGTAT CTACATCAAG GGATGAAATG GTTAATAGTT GTGGCAGTCC AGAATCAAAA 
CTGGATTTTG GTGCAGAGTG CCCTCATTTA AAACTGACAC ATAGCACATC TGCATGTTTA 
CACTCAAAAA ACAATTCAGA ATATCGGATT AATGGAAATG AACTAAAAAG AACTAATATT 
CAAATGAACA AAACCCACCA TTTTCTGCAA AGAAGCATGT CCTTAGAAGG TTCTTGCCAG 
GAAGTTTCTT GCTATTTATC TAGTCTTAAA TACAAGTGTC CTAAGGCAGG GGCATCTCAC 
TTGCACATAC AGTTCCGACC TCGGAATGAA GGAAAAACTA ATGGCTTTAA AAAAAGAAAT 
GGAATTTCCA AAAGGGAAAT GTGTAACAGG ACTTTGTGGA AGCCCCTCAA GACCAGAGAT 
GACTTCCTAG GCCAAGTGGA CGTTCCTCTT TATCAGTTAC CGACAGAAAA TCCAAGTATG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAAAGTT 
AAAGGTCATC TTAGATTAAA AATGACTTAC TTACCTAAAA ATAATGGATC TGAAGAAGAA 
AATACAGAAC AGGCAGAGGA ATTAGAGCCT GGCTGGATTA TATTGGACCA ACCAGATGCT 
GCTTGCCAGC TGCAACACCA GCAACAGGAA ATTCCTCCCC TACCTCCAGG CTGGGAAGAA 
AGGCAAGATA TACTTGGCAG GACTTATTAT GTCAACCATG AATTCAGAAG GACACAGTGG 
AAGAGACCAA CTGCTCAGGA CAGTACAGCT GATGCAGATC ATGGGAACTT TCAGTTGGAA 
GCACAGCATG CATTTACTCA TCGGCATCAA ATATCAGAAG ATTCTGAAAA TTTAGACAAC 
AGGGATTCTC CTGAGAGCTG GGAAATCATA ACTGAAGATG AGGCAACTAT GTATAGCAAT 
CAGAGTCTAC AAATGCCTCT GTCACAGAGT AATTCTGATG TACAGACTCA GCTTGCAGAA 
GAATTGAATA CCAGACTTAC TACCTCCGGG AGTTCAGCCA GTGGCCAACC AGCTCTTTAC 
ACTAATCATT CCAGCAGAAG AAGTAGTCTG CAGACGTACA GGGTTGAGGA ACAGCCTGCA 
CTTCCTGTGT TATTGCCTAC TTCATCTGGA TTGCCTCCAG GTTGGGAAGA AAGACAAGAT 
GAAAAAGGAC GATCATATTA CATAGATCAC AATTCCAGAA CTACTACCTG GATAAAGCCT 
GTTGTTCAGA CTACAGTAGA AACGGGTCAG CTATCAACTG TCCAGAGTAC TTCTACAGGA 
CAGCAGCCAC CATCAAGTGA TTCATCACAA TCTGAAACAG ATCAAGGATT ACTCCCTAAA 
GGCTGGGAAG TCCGACATGC ACCAAATGGA AGACCATTCT TTATTGATCA CAATACCAAA 
ACTACTACAT GGGAAGATCC AAGATTGAAA ATTCCAGCTC ATCTAAGAAG AAAGACCTCT 
CTAGATCCCA CAGATCTAGG CCCCTTGCCA CCAGGATGGG AAGAGAGAAC TCATACAGAT 
GGAAGAATAT TCTACATAAA CCACAACACA AAGAAAACAC AATGGGAAGA TCCACGACTA 
CAGACTGCAG CGATTACTGG ACCAGCTGTG CCTTACTCTA GGGATTACAA AAGAAAATAT 
GAATTCTTCA GAAAAAAGTT GAAGAAGCAG AGTGATATTC CAAATAGATT TGAAATGAAA 
GTTCATCGCA CAACAATCCT TGAAGATTCT TATAGAAGAA TTATAGCTGT AAAAAGAGCA 
GAATTCCTCA AAGCAAAACT CTGGATTGAG TTTGATGGTG AAAAAGGATT AGATTATGGA 
GGAGTGGCAA GGGAATGGTT CTTCCTTCTC TCTAAAGAAA TGTTTAATCC CTATTATGGA 
TTGTTTGAAT ATTCTGCTAC GGATAACTAT ACTCTGCAGA TCAATCCAAA TTCTGGCTTG 
TGCAACGAAG ATCATCTCTC TTACTTCAAA TTTATAGGTC GTGTAGCTGG AATGGCTGTT 
TACCATGGCA AGCTTTTGGA TGCCTTTTTT ATTCGGCCTT TTTACAAGAT GATGCTTCAA 
AAACCAATAA CGCTTCACGA CATGGAATCT GTGGACAGTG AATATTACAA TTCTTTGAAA 
TGGATTCTTG AAAATGACCC AGCAGAATTG GACCTCAGGT TTATGGTTGA TGAAGAGCTA 
TTTGGACAGA CTCATCAACA TGATTTGAAA AGTGGTGGAT CAGAAATAGT AGTTACCAAC 
AAGAACAAAC GAGACTATAT TCACCTTGTA ATTCAATGGA GGTTTGTGAG CAGAGTCCAG 
AAACAAATGG CTGCCTTTAA GGAGGGATTT TTTGAACTGA TACCACAGGA CCTAATTAAA 
ATTTTTGATG AAAATGAATT AGAGTTATTA ATGTGTGGAC TAGGAGATGT GGATGTGGCT 
GACTGGAAAC TACATACAAA ATACAAAAAT GGTTACAGCA TAAATCACCA AGTTATACAG 
TGGTTTTGGA AGGCAGTTTT GATGATGGAT TCCGAAAAGC GAATACGATT ACTTCAGTTT 
GTCACTGGTA CATCCCGTGT ACCTATGAAT GGATTTGCTG AATTATATGG TTCAAATGGA 
CCACAGTTGT TTACAATCGA ACAGTGGGGC ACCCCGGAAA AACTGCCAAG AGCTCATACT 
TGCTTTAATC GCTTGGATTT GCCTCCATAT GACTCATTTG AAGACTTATG GGAAAAACTC 
CTCTTAGCAA TTGAAAATAC TCAGGGTTTT GATGGAGTCG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019389071.1
CDS	384..4328
Translation	MARRLRLHFAARRSNTDPLSEASEDDLDNNIYMYFKRPSQNSASNVIEMKLTPRRAAFPPVMQEIINQQQSASGSSLQKNNKRSTSLQISLQSRKYSGCLQSGDIVATNEDSSFTGVGNEGNCSGRNIGDYPSNPINTTSHLSSTTMFNDSLGSIAASDNGSFSSAGSDYGSCTSTTSDGGSHCNSVLSDNSSCTLSTGDGTFYNSIAYDNSSYSRATNKYNSYWKDASQESLCQSNTIFDQDGLEQVKLSARRKTKVPLRRCSSLVIFPRSPSSTPPMSPTSSGPPPQSVAYQTSHKLVISPGDLAQHENQTGNKEFLSTAVNGLRLSKSAFSPGEVRAIRPLYLNSSLQAKSQATDSSEQATCEETSDGFSCISVHRPQKVSTSRDEMVNSCGSPESKLDFGAECPHLKLTHSTSACLHSKNNSEYRINGNELKRTNIQMNKTHHFLQRSMSLEGSCQEVSCYLSSLKYKCPKAGASHLHIQFRPRNEGKTNGFKKRNGISKREMCNRTLWKPLKTRDDFLGQVDVPLYQLPTENPSMERPYTFKDFVLHPRSHKSKVKGHLRLKMTYLPKNNGSEEENTEQAEELEPGWIILDQPDAACQLQHQQQEIPPLPPGWEERQDILGRTYYVNHEFRRTQWKRPTAQDSTADADHGNFQLEAQHAFTHRHQISEDSENLDNRDSPESWEIITEDEATMYSNQSLQMPLSQSNSDVQTQLAEELNTRLTTSGSSASGQPALYTNHSSRRSSLQTYRVEEQPALPVLLPTSSGLPPGWEERQDEKGRSYYIDHNSRTTTWIKPVVQTTVETGQLSTVQSTSTGQQPPSSDSSQSETDQGLLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRRKTSLDPTDLGPLPPGWEERTHTDGRIFYINHNTKKTQWEDPRLQTAAITGPAVPYSRDYKRKYEFFRKKLKKQSDIPNRFEMKVHRTTILEDSYRRIIAVKRAEFLKAKLWIEFDGEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLHDMESVDSEYYNSLKWILENDPAELDLRFMVDEELFGQTHQHDLKSGGSEIVVTNKNKRDYIHLVIQWRFVSRVQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVADWKLHTKYKNGYSINHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPEKLPRAHTCFNRLDLPPYDSFEDLWEKLLLAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_019533526.1
Organism	Crocodylus porosus(Australian saltwater crocodile)
Definition	Crocodylus porosus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019533526.1

ORF Insert Sequence:

ATGGCACGAA GGTTAAGATT GCATTTTGCA GCCCGAAGAA GCAACACCGA TCCACTGTCA 
GAAGCCTCTG AAGATGACTT GGATAATAAT ATTTATATGT ATTTCAAAAG ACCTTCTCAG 
AATTCTGCAT CTAATGTCAT AGAAATGAAA CTGACTCCCA GACGAGCAGC CTTTCCACCT 
GTAATGCAGG AAATAATTAA TCAACAACAA AGTGCAAGTG GTTCTTCGCT GCAAAAAAAC 
AACAAAAGGA GCACTTCACT TCAGATTTCC CTGCAGTCTA GAAAGTACAG TGGATGTCTT 
CAGTCTGGTG ATATAGTAGC TACTAATGAA GATTCATCAT TCACTGGTGT TGGCAATGAA 
GGGAACTGTT CTGGGAGAAA TATTGGTGAT TATCCGTCTA ACCCGATAAA TACCACCAGC 
CACTTGAGCA GTACTACCAT GTTCAATGAC AGTCTTGGCA GTATTGCAGC CAGTGACAAT 
GGATCCTTCA GTAGTGCTGG CAGTGACTAT GGATCATGCA CAAGTACCAC AAGTGATGGA 
GGTTCACACT GCAACAGTGT CCTTAGTGAC AATAGTAGTT GCACTCTTTC CACAGGTGAT 
GGTACTTTTT ATAATAGCAT TGCATATGAT AACTCTTCGT ATAGCAGAGC TACAAACAAA 
TATAATTCCT ATTGGAAAGA TGCATCTCAG GAAAGTTTGT GTCAAAGTAA TACTATTTTT 
GACCAAGATG GTTTGGAGCA AGTGAAATTG TCAGCGAGGA GGAAAACTAA AGTTCCACTA 
AGACGTTGTT CATCGTTGGT AATTTTCCCT CGAAGTCCTT CCAGTACTCC ACCAATGTCT 
CCAACAAGCT CAGGTCCTCC ACCACAGAGT GTGGCATATC AGACCTCACA TAAACTAGTT 
ATTTCTCCAG GTGATCTGGC ACAACATGAA AATCAAACTG GTAATAAGGA GTTCCTTTCA 
ACAGCAGTCA ATGGACTTCG ACTCTCTAAA TCTGCTTTCT CTCCTGGTGA GGTTAGAGCG 
ATTAGACCAC TTTACTTGAA TAGTTCATTA CAGGCCAAAA GTCAAGCCAC AGATAGTTCT 
GAGCAGGCAA CTTGTGAAGA AACATCTGAT GGCTTTTCTT GTATTTCAGT TCACCGTCCT 
CAAAAAGTAT CTACATCAAG GGATGAAATG GTTAATAGTT GTGGCAGTCC AGAATCAAAA 
CTGGATTTTG GTGCAGAGTG CCCTCATTTA AAACTGACAC ATAGCACATC TGCATGTTTA 
CACTCAAAAA ACAATTCAGA ATATCGGATT AATGGAAATG AACTAAAAAG AACTAATATT 
CAAATGAACA AAACCCACCA TTTTCTGCAA AGAAGCATGT CCTTAGAAGG TTCTTGCCAG 
GAAGTTTCTT GCTATTTATC TAGTCTTAAA TACAAGTGTC CTAAGGCAGG GGCATCTCAC 
TTGCACATAC AGTTCCGACC TCGGAATGAA GGAAAAACTA ATGGCTTTAA AAAAAGAAAT 
GGAATTTCCA AAAGGGAAAT GTGTAACAGG ACTTTGTGGA AGCCCCTCAA GACCAGAGAT 
GACTTCCTAG GCCAAGTGGA CGTTCCTCTT TATCAGTTAC CGACAGAAAA TCCAAGTATG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAAAGTT 
AAAGGTCATC TTAGATTAAA AATGACTTAC TTACCTAAAA ATAATGGATC TGAAGAAGAA 
AATACAGAAC AGGCAGAGGA ATTAGAGCCT GGCTGGATTA TATTGGACCA ACCAGATGCT 
GCTTGCCAGC TGCAACACCA GCAACAGGAA ATTCCTCCCC TACCTCCAGG CTGGGAAGAA 
AGGCAAGATA TACTTGGCAG GACTTATTAT GTCAACCATG AATTCAGAAG GACACAGTGG 
AAGAGACCAA CTGCTCAGGA CAGTACAGCT GATGCAGATC ATGGGAACTT TCAGTTGGAA 
GCACAGCATG CATTTACTCA TCGGCATCAA ATATCAGAAG ATTCTGAAAA TTTAGACAAC 
AGGGATTCTC CTGAGAGCTG GGAAATCATA ACTGAAGATG AGGCAACTAT GTATAGCAAT 
CAGAGTCTAC AAATGCCTCT GTCACAGAGT AATTCTGATG TACAGACTCA GCTTGCAGAA 
GAATTGAATA CCAGACTTAC TACCTCCGGG AGTTCAGCCA GTGGCCAACC AGCTCTTTAC 
ACTAATCATT CCAGCAGAAG AAGTAGTCTG CAGACGTACA GGGTTGAGGA ACAGCCTGCA 
CTTCCTGTGT TATTGCCTAC TTCATCTGGA TTGCCTCCAG GTTGGGAAGA AAGACAAGAT 
GAAAAAGGAC GATCATATTA CATAGATCAC AATTCCAGAA CTACTACCTG GATAAAGCCT 
GTTGTTCAGA CTACAGTAGA AACGGGTCAG CTATCAACTG TCCAGAGTAC TTCTACAGGA 
CAGCAGCCAC CATCAAGTGA TTCATCACAA TCTGAAACAG ATCAAGGATT ACTCCCTAAA 
GGCTGGGAAG TCCGACATGC ACCAAATGGA AGACCATTCT TTATTGATCA CAATACCAAA 
ACTACTACAT GGGAAGATCC AAGATTGAAA ATTCCAGCTC ATCTAAGAAG AAAGACCTCT 
CTAGATCCCA CAGATCTAGG CCCCTTGCCA CCAGGATGGG AAGAGAGAAC TCATACAGAT 
GGAAGAATAT TCTACATAAA CCACAACACA AAGAAAACAC AATGGGAAGA TCCACGACTA 
CAGACTGCAG CGATTACTGG ACCAGCTGTG CCTTACTCTA GGGATTACAA AAGAAAATAT 
GAATTCTTCA GAAAAAAGTT GAAGAAGCAG AGTGATATTC CAAATAGATT TGAAATGAAA 
GTTCATCGCA CAACAATCCT TGAAGATTCT TATAGAAGAA TTATAGCTGT AAAAAGAGCA 
GAATTCCTCA AAGCAAAACT CTGGATTGAG TTTGATGGTG AAAAAGGATT AGATTATGGA 
GGAGTGGCAA GGGAATGGTT CTTCCTTCTC TCTAAAGAAA TGTTTAATCC CTATTATGGA 
TTGTTTGAAT ATTCTGCTAC GGATAACTAT ACTCTGCAGA TCAATCCAAA TTCTGGCTTG 
TGCAACGAAG ATCATCTCTC TTACTTCAAA TTTATAGGTC GTGTAGCTGG AATGGCTGTT 
TACCATGGCA AGCTTTTGGA TGCCTTTTTT ATTCGGCCTT TTTACAAGAT GATGCTTCAA 
AAACCAATAA CGCTTCACGA CATGGAATCT GTGGACAGTG AATATTACAA TTCTTTGAAA 
TGGATTCTTG AAAATGACCC AGCAGAATTG GACCTCAGGT TTATGGTTGA TGAAGAGCTA 
TTTGGACAGA CTCATCAACA TGATTTGAAA AGTGGTGGAT CAGAAATAGT AGTTACCAAC 
AAGAACAAAC GAGACTATAT TCACCTTGTA ATTCAATGGA GGTTTGTGAG CAGAGTCCAG 
AAACAAATGG CTGCCTTTAA GGAGGGATTT TTTGAACTGA TACCACAGGA CCTAATTAAA 
ATTTTTGATG AAAATGAATT AGAGTTATTA ATGTGTGGAC TAGGAGATGT GGATGTGGCT 
GACTGGAAAC TACATACAAA ATACAAAAAT GGTTACAGCA TAAATCACCA AGTTATACAG 
TGGTTTTGGA AGGCAGTTTT GATGATGGAT TCCGAAAAGC GAATACGATT ACTTCAGTTT 
GTCACTGGTA CATCCCGTGT ACCTATGAAT GGATTTGCTG AATTATATGG TTCAAATGGA 
CCACAGTTGT TTACAATCGA ACAGTGGGGC ACCCCGGAAA AACTGCCAAG AGCTCATACT 
TGCTTTAATC GCTTGGATTT GCCTCCATAT GACTCATTTG AAGACTTATG GGAAAAACTC 
CTCTTAGCAA TTGAAAATAC TCAGGGTTTT GATGGAGTCG ATTAG