LOC105341856 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105341856
Entrez Gene ID	105341856
Full Name	diacylglycerol kinase theta
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011448603.2	XP_011446905.1	diacylglycerol kinase theta isoform X1
XM_011448604.2	XP_011446906.1	diacylglycerol kinase theta isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105341856 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105341856 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr108551	XM_011448603.2 Latest version!	Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCr108552	XM_011448604.2 Latest version!	Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105341856 ( XM_011448603.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011446905.1 Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr108551
Clone ID Related Accession (Same CDS sequence)	XM_011448603.2
Accession Version	XM_011448603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	diacylglycerol kinase theta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936606.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011448603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGTA TTCGATTAAG TAGGCGAAGA GTGTGTCTTT CAATGGAGAA CAACACAGTG 
GATGGCGGAA TTGTTGATGA AGAGAGGACG TCCGGTTTTA TGCCTGAAAA TGAGCAGGGG 
CATGGACATT CGTTCATCAA AAAGACATTT CATAAGCCCA CGTATTGTCA CCATTGCACC 
CTGATGCTAT GGGGTCTCAT AGGACAAGGA TATGTATGCG AAGTTTGCAA CTTTGTGGTC 
CATGAGCGAT GTCTGAAATT AGTGGTGTCT GCCTGCTCCA GCATTGCCAC AACTCTGATC 
AAGAATCCAG TGGCTCATTG TTTCTCGACA CCCTCCATCT TCAAACGCAA GTTCTGTGAT 
GTATGTCGCA AGCGGCTGGA GGACAGTGCA GCCATCAGAT GTGAACTGTG TGAATACTAT 
GCCCATGTGG ATTGCCAAGA CTTTGTGGTC AGCAATTGTA AGGAGTGCGC CACCTACACC 
CCGGACAATG ATCGTGTTGG CCAACAGAAC ATTGTGCAAT ACCATCACTG GAGGGAGGGG 
AACTTGCCAG CTAACTCCAA ATGTGTAGCG TGTAAAAAGA CCTGCTGGTC ATCTGAATGT 
ATGGCCGGCA TGAGGTGTGA ATGGTGTGGT GTCACGTCCC ATGCAAGTTG CTACAAACTT 
CTTCCAGCAG AATGTAATTT TGGGAGTTTA CGAGAAATCA TGCTGCCTCC AAGTTGTTTG 
ACAGTCCCCA GGATAAACAT CAGCATGGAT AAGATCCTAG GAATCAACGT CAGGACTCCC 
GACGAACCAG AGAAGATAGA GAAAGCCCAA AAATCGAAGC CCAAGAAGGG TAGTCGAAAT 
GGGACATCTG AAGAATCGGA TAGCTATCTC ATAAAACCAG TTTTCGATGA CTGTCCATCC 
AGTGGAGATT CCAAAAACGA GGAGGAGGAG AGAGAACGGA AGTCGCCTCG TGAAAAAGAG 
TACAAGGACA AAGATAAAGA CCCAGATCTG GAAGTTTTCC ATGTGTATGA TGGGGATAAT 
TCATTAAGAG AAAAGAGATA CCGGACAGTA TCTGTTCCAA AGAATGCTTC CTTTCAAAGT 
ATATTGGAAG CAACATTGAA AACATTCCAC ATATCAGATG ATCCGAAGAA CTATTATGTC 
TCAGAAGTGA CAGACTCAGA AGAGAGGGAG CTAGATGAGA ATAGTCCAAT CAGATCACAG 
CTGAAGACGT CAGACGACAA GAGACCCTCC ATTTTCCTAC GTTATCGAGA CATGGACTCG 
GAAAGGGGCA TGATCAAAGT CTACCCAGGA GTGTTGAGGG TATCAGGTAG TTACAAAAAT 
ATAGAGATTG GCTCCGACAT GACCTGTGTG GATGTTATAC GAGAGGCTCT TCAAAAGTTT 
GGTGTGGAGG ATGTGGTCCC GGAGACCTTC AACTTGATTG AGGTTCTGTT GGATAAAGGG 
GTCAATGAAC AGGTGTTACC AAGTGATGCA AGGCCATGGC AGATTCTGCA GAAGAGTCGA 
AAGGACTCCT TACAACGGAA CAGGATGACC AGGTTTTACC TAAAGCAGAG GGAGGACCCC 
CATGGTCCCA GTATCTCACT GTTTGTAGGA AATCTACCCA CAGGCTTGGC TCAACGACAA 
TATGAAAAAC TTCTTTTAGA TTTTATAACT CCAGAGAATA AATGGTCCTA TTTTGATGTG 
ATATACTATG AATATGGGTC TCTAGTGCTA ATATACAACT CTGCAGAACA TGCAACCAGT 
GCATTCAACA TTTTGAGTAA TAGTACATTT GATCAGAAAC CAATATTAGC ATTGCTTTTA 
CCCAATATTC AGTCCCACAT GATCTTAGAA AACGTCAGCC CTCTGCTAGT TTTTGTCAAT 
GCAAAGAGTG GAGGCTGTCA GGGCTCTGAG CTCATCACTT CCTTCCGCAA ACTTCTGAAC 
CCCCATCAGG TGTTTAACTT GGAGAATGGG GGTCCTCTGC CCGGCCTGTA TGTGTTCAGA 
CACGTAGCCC ACTACAAGAT CCTGGCCTGT GGGGGGGACG GGACCGTGGG CTGGGTCCTC 
TCCTGTCTGG ACAATGTGGG ACAGGATGCG GTGTGCCAGT CGCCTCCCCT GGCTATTGTT 
CCATTGGGTA CAGGTAATGA TTTGGCAAGA GTTTTACGCT GGGGACCAGG TTACACAGGT 
GGGGAGGACC CGTTAAATTT ACTAAGGGAC GTTATAGATG CTGAGGAAAT AAAACTAGAC 
AGGTGGACTG TGATCTTCCA TCCAAACGAG AAGGAACAAG AAACCAAAGA CCAGTATGAG 
GACACAACCT CAATATTTGT AATGAACAAT TATTTTGGTA TTGGAATCGA TGCTGAAATA 
TCTTTGGACT TCCACACAGC CAGAGAAGAG AAACCAGGAA AATTCCAAAG CAGAATTCAT 
AATAAAGGGT TTTATTTTAA AATGGGATTA CAAAAAATGG TAAAGAAAAG ACTGGTGAAA 
GACTTGCATC GCCACATTAG ACTAGAGGTG GATGGGCGAC TAGTAGAGTT ACCACCAGTT 
GAGGGAATAA TTATACTAAA TATACTCAGT TGGGGGTCTG GCTCCAACCC TTGGGGACCG 
GAAAGGGAGG ACCAATTTGC TAAACCAACT CATTACGACG GGATGCTAGA GGTCGTGGGG 
GTCACTGGAG TAGTTCATAT GGGACAGATC CAGAGCAGTC TACGAGCCGG AATCCGTATA 
GCACAGGGAG GTCATTTAAG GATCACATTA CTGACAGACA TACCTGTGCA GGTAGATGGA 
GAGCCCTGGG TACAACCTGC AGGACAAGTT GTAGTTCTAA GATCAGCATT AAAGGCAACA 
ATGCTAAAAA AGAGCAAAAA GAAGATGAGG CGGAGAAATA CAGAACCTAG CATATTTTTT 
CCTGACAATG AACCTCTGCG TTTACAGTCA CCCACAGAGG AGAGTGAACC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011446905.1
CDS	282..3218
Translation	MPCIRLSRRRVCLSMENNTVDGGIVDEERTSGFMPENEQGHGHSFIKKTFHKPTYCHHCTLMLWGLIGQGYVCEVCNFVVHERCLKLVVSACSSIATTLIKNPVAHCFSTPSIFKRKFCDVCRKRLEDSAAIRCELCEYYAHVDCQDFVVSNCKECATYTPDNDRVGQQNIVQYHHWREGNLPANSKCVACKKTCWSSECMAGMRCEWCGVTSHASCYKLLPAECNFGSLREIMLPPSCLTVPRINISMDKILGINVRTPDEPEKIEKAQKSKPKKGSRNGTSEESDSYLIKPVFDDCPSSGDSKNEEEERERKSPREKEYKDKDKDPDLEVFHVYDGDNSLREKRYRTVSVPKNASFQSILEATLKTFHISDDPKNYYVSEVTDSEERELDENSPIRSQLKTSDDKRPSIFLRYRDMDSERGMIKVYPGVLRVSGSYKNIEIGSDMTCVDVIREALQKFGVEDVVPETFNLIEVLLDKGVNEQVLPSDARPWQILQKSRKDSLQRNRMTRFYLKQREDPHGPSISLFVGNLPTGLAQRQYEKLLLDFITPENKWSYFDVIYYEYGSLVLIYNSAEHATSAFNILSNSTFDQKPILALLLPNIQSHMILENVSPLLVFVNAKSGGCQGSELITSFRKLLNPHQVFNLENGGPLPGLYVFRHVAHYKILACGGDGTVGWVLSCLDNVGQDAVCQSPPLAIVPLGTGNDLARVLRWGPGYTGGEDPLNLLRDVIDAEEIKLDRWTVIFHPNEKEQETKDQYEDTTSIFVMNNYFGIGIDAEISLDFHTAREEKPGKFQSRIHNKGFYFKMGLQKMVKKRLVKDLHRHIRLEVDGRLVELPPVEGIIILNILSWGSGSNPWGPEREDQFAKPTHYDGMLEVVGVTGVVHMGQIQSSLRAGIRIAQGGHLRITLLTDIPVQVDGEPWVQPAGQVVVLRSALKATMLKKSKKKMRRRNTEPSIFFPDNEPLRLQSPTEESEPL

Target ORF information:

RefSeq Version	XM_011448603.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011448603.2

ORF Insert Sequence:

ATGCCATGTA TTCGATTAAG TAGGCGAAGA GTGTGTCTTT CAATGGAGAA CAACACAGTG 
GATGGCGGAA TTGTTGATGA AGAGAGGACG TCCGGTTTTA TGCCTGAAAA TGAGCAGGGG 
CATGGACATT CGTTCATCAA AAAGACATTT CATAAGCCCA CGTATTGTCA CCATTGCACC 
CTGATGCTAT GGGGTCTCAT AGGACAAGGA TATGTATGCG AAGTTTGCAA CTTTGTGGTC 
CATGAGCGAT GTCTGAAATT AGTGGTGTCT GCCTGCTCCA GCATTGCCAC AACTCTGATC 
AAGAATCCAG TGGCTCATTG TTTCTCGACA CCCTCCATCT TCAAACGCAA GTTCTGTGAT 
GTATGTCGCA AGCGGCTGGA GGACAGTGCA GCCATCAGAT GTGAACTGTG TGAATACTAT 
GCCCATGTGG ATTGCCAAGA CTTTGTGGTC AGCAATTGTA AGGAGTGCGC CACCTACACC 
CCGGACAATG ATCGTGTTGG CCAACAGAAC ATTGTGCAAT ACCATCACTG GAGGGAGGGG 
AACTTGCCAG CTAACTCCAA ATGTGTAGCG TGTAAAAAGA CCTGCTGGTC ATCTGAATGT 
ATGGCCGGCA TGAGGTGTGA ATGGTGTGGT GTCACGTCCC ATGCAAGTTG CTACAAACTT 
CTTCCAGCAG AATGTAATTT TGGGAGTTTA CGAGAAATCA TGCTGCCTCC AAGTTGTTTG 
ACAGTCCCCA GGATAAACAT CAGCATGGAT AAGATCCTAG GAATCAACGT CAGGACTCCC 
GACGAACCAG AGAAGATAGA GAAAGCCCAA AAATCGAAGC CCAAGAAGGG TAGTCGAAAT 
GGGACATCTG AAGAATCGGA TAGCTATCTC ATAAAACCAG TTTTCGATGA CTGTCCATCC 
AGTGGAGATT CCAAAAACGA GGAGGAGGAG AGAGAACGGA AGTCGCCTCG TGAAAAAGAG 
TACAAGGACA AAGATAAAGA CCCAGATCTG GAAGTTTTCC ATGTGTATGA TGGGGATAAT 
TCATTAAGAG AAAAGAGATA CCGGACAGTA TCTGTTCCAA AGAATGCTTC CTTTCAAAGT 
ATATTGGAAG CAACATTGAA AACATTCCAC ATATCAGATG ATCCGAAGAA CTATTATGTC 
TCAGAAGTGA CAGACTCAGA AGAGAGGGAG CTAGATGAGA ATAGTCCAAT CAGATCACAG 
CTGAAGACGT CAGACGACAA GAGACCCTCC ATTTTCCTAC GTTATCGAGA CATGGACTCG 
GAAAGGGGCA TGATCAAAGT CTACCCAGGA GTGTTGAGGG TATCAGGTAG TTACAAAAAT 
ATAGAGATTG GCTCCGACAT GACCTGTGTG GATGTTATAC GAGAGGCTCT TCAAAAGTTT 
GGTGTGGAGG ATGTGGTCCC GGAGACCTTC AACTTGATTG AGGTTCTGTT GGATAAAGGG 
GTCAATGAAC AGGTGTTACC AAGTGATGCA AGGCCATGGC AGATTCTGCA GAAGAGTCGA 
AAGGACTCCT TACAACGGAA CAGGATGACC AGGTTTTACC TAAAGCAGAG GGAGGACCCC 
CATGGTCCCA GTATCTCACT GTTTGTAGGA AATCTACCCA CAGGCTTGGC TCAACGACAA 
TATGAAAAAC TTCTTTTAGA TTTTATAACT CCAGAGAATA AATGGTCCTA TTTTGATGTG 
ATATACTATG AATATGGGTC TCTAGTGCTA ATATACAACT CTGCAGAACA TGCAACCAGT 
GCATTCAACA TTTTGAGTAA TAGTACATTT GATCAGAAAC CAATATTAGC ATTGCTTTTA 
CCCAATATTC AGTCCCACAT GATCTTAGAA AACGTCAGCC CTCTGCTAGT TTTTGTCAAT 
GCAAAGAGTG GAGGCTGTCA GGGCTCTGAG CTCATCACTT CCTTCCGCAA ACTTCTGAAC 
CCCCATCAGG TGTTTAACTT GGAGAATGGG GGTCCTCTGC CCGGCCTGTA TGTGTTCAGA 
CACGTAGCCC ACTACAAGAT CCTGGCCTGT GGGGGGGACG GGACCGTGGG CTGGGTCCTC 
TCCTGTCTGG ACAATGTGGG ACAGGATGCG GTGTGCCAGT CGCCTCCCCT GGCTATTGTT 
CCATTGGGTA CAGGTAATGA TTTGGCAAGA GTTTTACGCT GGGGACCAGG TTACACAGGT 
GGGGAGGACC CGTTAAATTT ACTAAGGGAC GTTATAGATG CTGAGGAAAT AAAACTAGAC 
AGGTGGACTG TGATCTTCCA TCCAAACGAG AAGGAACAAG AAACCAAAGA CCAGTATGAG 
GACACAACCT CAATATTTGT AATGAACAAT TATTTTGGTA TTGGAATCGA TGCTGAAATA 
TCTTTGGACT TCCACACAGC CAGAGAAGAG AAACCAGGAA AATTCCAAAG CAGAATTCAT 
AATAAAGGGT TTTATTTTAA AATGGGATTA CAAAAAATGG TAAAGAAAAG ACTGGTGAAA 
GACTTGCATC GCCACATTAG ACTAGAGGTG GATGGGCGAC TAGTAGAGTT ACCACCAGTT 
GAGGGAATAA TTATACTAAA TATACTCAGT TGGGGGTCTG GCTCCAACCC TTGGGGACCG 
GAAAGGGAGG ACCAATTTGC TAAACCAACT CATTACGACG GGATGCTAGA GGTCGTGGGG 
GTCACTGGAG TAGTTCATAT GGGACAGATC CAGAGCAGTC TACGAGCCGG AATCCGTATA 
GCACAGGGAG GTCATTTAAG GATCACATTA CTGACAGACA TACCTGTGCA GGTAGATGGA 
GAGCCCTGGG TACAACCTGC AGGACAAGTT GTAGTTCTAA GATCAGCATT AAAGGCAACA 
ATGCTAAAAA AGAGCAAAAA GAAGATGAGG CGGAGAAATA CAGAACCTAG CATATTTTTT 
CCTGACAATG AACCTCTGCG TTTACAGTCA CCCACAGAGG AGAGTGAACC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105341856 ( XM_011448604.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011446906.1 Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr108552
Clone ID Related Accession (Same CDS sequence)	XM_011448604.2
Accession Version	XM_011448604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	diacylglycerol kinase theta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936606.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011448604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGTA TTCGATTAAG TAGGCGAAGA GTGTGTCTTT CAATGGAGAA CAACACAGTG 
GATGGCGGAA TTGTTGATGA AGAGAGGACG TCCGGTTTTA TGCCTGAAAA TGAGCAGGGG 
CATGGACATT CGTTCATCAA AAAGACATTT CATAAGCCCA CGTATTGTCA CCATTGCACC 
CTGATGCTAT GGGGTCTCAT AGGACAAGGA TATGTATGCG AAGTTTGCAA CTTTGTGGTC 
CATGAGCGAT GTCTGAAATT AGTGGTGTCT GCCTGCTCCA GCATTGCCAC AACTCTGATC 
AAGAATCCAG TGGCTCATTG TTTCTCGACA CCCTCCATCT TCAAACGCAA GTTCTGTGAT 
GTATGTCGCA AGCGGCTGGA GGACAGTGCA GCCATCAGAT GTGAACTGTG TGAATACTAT 
GCCCATGTGG ATTGCCAAGA CTTTGTGGTC AGCAATTGTA AGGAGTGCGC CACCTACACC 
CCGGACAATG ATCGTGTTGG CCAACAGAAC ATTGTGCAAT ACCATCACTG GAGGGAGGGG 
AACTTGCCAG CTAACTCCAA ATGTGTAGCG TGTAAAAAGA CCTGCTGGTC ATCTGAATGT 
ATGGCCGGCA TGAGGTGTGA ATGGTGTGGT GTCACGTCCC ATGCAAGTTG CTACAAACTT 
CTTCCAGCAG AATGTAATTT TGGGAGTTTA CGAGAAATCA TGCTGCCTCC AAGTTGTTTG 
ACAGTCCCCA GGATAAACAT CAGCATGGAT AAGATCCTAG GAATCAACGT CAGGACTCCC 
GACGAACCAG AGAAGATAGA GAAAGCCCAA AAATCGAAGC CCAAGAAGGG TAGTCGAAAT 
GGGACATCTG AAGAATCGGA TAGCTATCTC ATAAAACCAG TTTTCGATGA CTGTCCATCC 
AGTGGAGATT CCAAAAACGA GGAGGAGGAG AGAGAACGGA AGTCGCCTCG TGAAAAAGAG 
TACAAGGACA AAGATAAAGA CCCAGATCTG GAAGTTTTCC ATGTGTATGA TGGGGATAAT 
TCATTAAGAG AAAAGAGATA CCGGACAGTA TCTGTTCCAA AGAATGCTTC CTTTCAAAGT 
ATATTGGAAG CAACATTGAA AACATTCCAC ATATCAGATG ATCCGAAGAA CTATTATGTC 
TCAGAAGTGA CAGACTCAGA GAGGGAGCTA GATGAGAATA GTCCAATCAG ATCACAGCTG 
AAGACGTCAG ACGACAAGAG ACCCTCCATT TTCCTACGTT ATCGAGACAT GGACTCGGAA 
AGGGGCATGA TCAAAGTCTA CCCAGGAGTG TTGAGGGTAT CAGGTAGTTA CAAAAATATA 
GAGATTGGCT CCGACATGAC CTGTGTGGAT GTTATACGAG AGGCTCTTCA AAAGTTTGGT 
GTGGAGGATG TGGTCCCGGA GACCTTCAAC TTGATTGAGG TTCTGTTGGA TAAAGGGGTC 
AATGAACAGG TGTTACCAAG TGATGCAAGG CCATGGCAGA TTCTGCAGAA GAGTCGAAAG 
GACTCCTTAC AACGGAACAG GATGACCAGG TTTTACCTAA AGCAGAGGGA GGACCCCCAT 
GGTCCCAGTA TCTCACTGTT TGTAGGAAAT CTACCCACAG GCTTGGCTCA ACGACAATAT 
GAAAAACTTC TTTTAGATTT TATAACTCCA GAGAATAAAT GGTCCTATTT TGATGTGATA 
TACTATGAAT ATGGGTCTCT AGTGCTAATA TACAACTCTG CAGAACATGC AACCAGTGCA 
TTCAACATTT TGAGTAATAG TACATTTGAT CAGAAACCAA TATTAGCATT GCTTTTACCC 
AATATTCAGT CCCACATGAT CTTAGAAAAC GTCAGCCCTC TGCTAGTTTT TGTCAATGCA 
AAGAGTGGAG GCTGTCAGGG CTCTGAGCTC ATCACTTCCT TCCGCAAACT TCTGAACCCC 
CATCAGGTGT TTAACTTGGA GAATGGGGGT CCTCTGCCCG GCCTGTATGT GTTCAGACAC 
GTAGCCCACT ACAAGATCCT GGCCTGTGGG GGGGACGGGA CCGTGGGCTG GGTCCTCTCC 
TGTCTGGACA ATGTGGGACA GGATGCGGTG TGCCAGTCGC CTCCCCTGGC TATTGTTCCA 
TTGGGTACAG GTAATGATTT GGCAAGAGTT TTACGCTGGG GACCAGGTTA CACAGGTGGG 
GAGGACCCGT TAAATTTACT AAGGGACGTT ATAGATGCTG AGGAAATAAA ACTAGACAGG 
TGGACTGTGA TCTTCCATCC AAACGAGAAG GAACAAGAAA CCAAAGACCA GTATGAGGAC 
ACAACCTCAA TATTTGTAAT GAACAATTAT TTTGGTATTG GAATCGATGC TGAAATATCT 
TTGGACTTCC ACACAGCCAG AGAAGAGAAA CCAGGAAAAT TCCAAAGCAG AATTCATAAT 
AAAGGGTTTT ATTTTAAAAT GGGATTACAA AAAATGGTAA AGAAAAGACT GGTGAAAGAC 
TTGCATCGCC ACATTAGACT AGAGGTGGAT GGGCGACTAG TAGAGTTACC ACCAGTTGAG 
GGAATAATTA TACTAAATAT ACTCAGTTGG GGGTCTGGCT CCAACCCTTG GGGACCGGAA 
AGGGAGGACC AATTTGCTAA ACCAACTCAT TACGACGGGA TGCTAGAGGT CGTGGGGGTC 
ACTGGAGTAG TTCATATGGG ACAGATCCAG AGCAGTCTAC GAGCCGGAAT CCGTATAGCA 
CAGGGAGGTC ATTTAAGGAT CACATTACTG ACAGACATAC CTGTGCAGGT AGATGGAGAG 
CCCTGGGTAC AACCTGCAGG ACAAGTTGTA GTTCTAAGAT CAGCATTAAA GGCAACAATG 
CTAAAAAAGA GCAAAAAGAA GATGAGGCGG AGAAATACAG AACCTAGCAT ATTTTTTCCT 
GACAATGAAC CTCTGCGTTT ACAGTCACCC ACAGAGGAGA GTGAACCACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011446906.1
CDS	283..3216
Translation	MPCIRLSRRRVCLSMENNTVDGGIVDEERTSGFMPENEQGHGHSFIKKTFHKPTYCHHCTLMLWGLIGQGYVCEVCNFVVHERCLKLVVSACSSIATTLIKNPVAHCFSTPSIFKRKFCDVCRKRLEDSAAIRCELCEYYAHVDCQDFVVSNCKECATYTPDNDRVGQQNIVQYHHWREGNLPANSKCVACKKTCWSSECMAGMRCEWCGVTSHASCYKLLPAECNFGSLREIMLPPSCLTVPRINISMDKILGINVRTPDEPEKIEKAQKSKPKKGSRNGTSEESDSYLIKPVFDDCPSSGDSKNEEEERERKSPREKEYKDKDKDPDLEVFHVYDGDNSLREKRYRTVSVPKNASFQSILEATLKTFHISDDPKNYYVSEVTDSERELDENSPIRSQLKTSDDKRPSIFLRYRDMDSERGMIKVYPGVLRVSGSYKNIEIGSDMTCVDVIREALQKFGVEDVVPETFNLIEVLLDKGVNEQVLPSDARPWQILQKSRKDSLQRNRMTRFYLKQREDPHGPSISLFVGNLPTGLAQRQYEKLLLDFITPENKWSYFDVIYYEYGSLVLIYNSAEHATSAFNILSNSTFDQKPILALLLPNIQSHMILENVSPLLVFVNAKSGGCQGSELITSFRKLLNPHQVFNLENGGPLPGLYVFRHVAHYKILACGGDGTVGWVLSCLDNVGQDAVCQSPPLAIVPLGTGNDLARVLRWGPGYTGGEDPLNLLRDVIDAEEIKLDRWTVIFHPNEKEQETKDQYEDTTSIFVMNNYFGIGIDAEISLDFHTAREEKPGKFQSRIHNKGFYFKMGLQKMVKKRLVKDLHRHIRLEVDGRLVELPPVEGIIILNILSWGSGSNPWGPEREDQFAKPTHYDGMLEVVGVTGVVHMGQIQSSLRAGIRIAQGGHLRITLLTDIPVQVDGEPWVQPAGQVVVLRSALKATMLKKSKKKMRRRNTEPSIFFPDNEPLRLQSPTEESEPL

Target ORF information:

RefSeq Version	XM_011448604.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas diacylglycerol kinase theta (LOC105341856), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011448604.2

ORF Insert Sequence:

ATGCCATGTA TTCGATTAAG TAGGCGAAGA GTGTGTCTTT CAATGGAGAA CAACACAGTG 
GATGGCGGAA TTGTTGATGA AGAGAGGACG TCCGGTTTTA TGCCTGAAAA TGAGCAGGGG 
CATGGACATT CGTTCATCAA AAAGACATTT CATAAGCCCA CGTATTGTCA CCATTGCACC 
CTGATGCTAT GGGGTCTCAT AGGACAAGGA TATGTATGCG AAGTTTGCAA CTTTGTGGTC 
CATGAGCGAT GTCTGAAATT AGTGGTGTCT GCCTGCTCCA GCATTGCCAC AACTCTGATC 
AAGAATCCAG TGGCTCATTG TTTCTCGACA CCCTCCATCT TCAAACGCAA GTTCTGTGAT 
GTATGTCGCA AGCGGCTGGA GGACAGTGCA GCCATCAGAT GTGAACTGTG TGAATACTAT 
GCCCATGTGG ATTGCCAAGA CTTTGTGGTC AGCAATTGTA AGGAGTGCGC CACCTACACC 
CCGGACAATG ATCGTGTTGG CCAACAGAAC ATTGTGCAAT ACCATCACTG GAGGGAGGGG 
AACTTGCCAG CTAACTCCAA ATGTGTAGCG TGTAAAAAGA CCTGCTGGTC ATCTGAATGT 
ATGGCCGGCA TGAGGTGTGA ATGGTGTGGT GTCACGTCCC ATGCAAGTTG CTACAAACTT 
CTTCCAGCAG AATGTAATTT TGGGAGTTTA CGAGAAATCA TGCTGCCTCC AAGTTGTTTG 
ACAGTCCCCA GGATAAACAT CAGCATGGAT AAGATCCTAG GAATCAACGT CAGGACTCCC 
GACGAACCAG AGAAGATAGA GAAAGCCCAA AAATCGAAGC CCAAGAAGGG TAGTCGAAAT 
GGGACATCTG AAGAATCGGA TAGCTATCTC ATAAAACCAG TTTTCGATGA CTGTCCATCC 
AGTGGAGATT CCAAAAACGA GGAGGAGGAG AGAGAACGGA AGTCGCCTCG TGAAAAAGAG 
TACAAGGACA AAGATAAAGA CCCAGATCTG GAAGTTTTCC ATGTGTATGA TGGGGATAAT 
TCATTAAGAG AAAAGAGATA CCGGACAGTA TCTGTTCCAA AGAATGCTTC CTTTCAAAGT 
ATATTGGAAG CAACATTGAA AACATTCCAC ATATCAGATG ATCCGAAGAA CTATTATGTC 
TCAGAAGTGA CAGACTCAGA GAGGGAGCTA GATGAGAATA GTCCAATCAG ATCACAGCTG 
AAGACGTCAG ACGACAAGAG ACCCTCCATT TTCCTACGTT ATCGAGACAT GGACTCGGAA 
AGGGGCATGA TCAAAGTCTA CCCAGGAGTG TTGAGGGTAT CAGGTAGTTA CAAAAATATA 
GAGATTGGCT CCGACATGAC CTGTGTGGAT GTTATACGAG AGGCTCTTCA AAAGTTTGGT 
GTGGAGGATG TGGTCCCGGA GACCTTCAAC TTGATTGAGG TTCTGTTGGA TAAAGGGGTC 
AATGAACAGG TGTTACCAAG TGATGCAAGG CCATGGCAGA TTCTGCAGAA GAGTCGAAAG 
GACTCCTTAC AACGGAACAG GATGACCAGG TTTTACCTAA AGCAGAGGGA GGACCCCCAT 
GGTCCCAGTA TCTCACTGTT TGTAGGAAAT CTACCCACAG GCTTGGCTCA ACGACAATAT 
GAAAAACTTC TTTTAGATTT TATAACTCCA GAGAATAAAT GGTCCTATTT TGATGTGATA 
TACTATGAAT ATGGGTCTCT AGTGCTAATA TACAACTCTG CAGAACATGC AACCAGTGCA 
TTCAACATTT TGAGTAATAG TACATTTGAT CAGAAACCAA TATTAGCATT GCTTTTACCC 
AATATTCAGT CCCACATGAT CTTAGAAAAC GTCAGCCCTC TGCTAGTTTT TGTCAATGCA 
AAGAGTGGAG GCTGTCAGGG CTCTGAGCTC ATCACTTCCT TCCGCAAACT TCTGAACCCC 
CATCAGGTGT TTAACTTGGA GAATGGGGGT CCTCTGCCCG GCCTGTATGT GTTCAGACAC 
GTAGCCCACT ACAAGATCCT GGCCTGTGGG GGGGACGGGA CCGTGGGCTG GGTCCTCTCC 
TGTCTGGACA ATGTGGGACA GGATGCGGTG TGCCAGTCGC CTCCCCTGGC TATTGTTCCA 
TTGGGTACAG GTAATGATTT GGCAAGAGTT TTACGCTGGG GACCAGGTTA CACAGGTGGG 
GAGGACCCGT TAAATTTACT AAGGGACGTT ATAGATGCTG AGGAAATAAA ACTAGACAGG 
TGGACTGTGA TCTTCCATCC AAACGAGAAG GAACAAGAAA CCAAAGACCA GTATGAGGAC 
ACAACCTCAA TATTTGTAAT GAACAATTAT TTTGGTATTG GAATCGATGC TGAAATATCT 
TTGGACTTCC ACACAGCCAG AGAAGAGAAA CCAGGAAAAT TCCAAAGCAG AATTCATAAT 
AAAGGGTTTT ATTTTAAAAT GGGATTACAA AAAATGGTAA AGAAAAGACT GGTGAAAGAC 
TTGCATCGCC ACATTAGACT AGAGGTGGAT GGGCGACTAG TAGAGTTACC ACCAGTTGAG 
GGAATAATTA TACTAAATAT ACTCAGTTGG GGGTCTGGCT CCAACCCTTG GGGACCGGAA 
AGGGAGGACC AATTTGCTAA ACCAACTCAT TACGACGGGA TGCTAGAGGT CGTGGGGGTC 
ACTGGAGTAG TTCATATGGG ACAGATCCAG AGCAGTCTAC GAGCCGGAAT CCGTATAGCA 
CAGGGAGGTC ATTTAAGGAT CACATTACTG ACAGACATAC CTGTGCAGGT AGATGGAGAG 
CCCTGGGTAC AACCTGCAGG ACAAGTTGTA GTTCTAAGAT CAGCATTAAA GGCAACAATG 
CTAAAAAAGA GCAAAAAGAA GATGAGGCGG AGAAATACAG AACCTAGCAT ATTTTTTCCT 
GACAATGAAC CTCTGCGTTT ACAGTCACCC ACAGAGGAGA GTGAACCACT CTAA