LOC105340402 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105340402
Entrez Gene ID	105340402
Full Name	probable E3 ubiquitin-protein ligase HERC4
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011446428.2	XP_011444730.1	probable E3 ubiquitin-protein ligase HERC4
XM_011446427.2	XP_011444729.1	probable E3 ubiquitin-protein ligase HERC4

Pathways from BioSystems
Gene Ontology

The following LOC105340402 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105340402 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr106953	XM_011446428.2 Latest version!	Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCr106953	XM_011446427.2 Latest version!	Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105340402 ( XM_011446428.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011444730.1 Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr106953
Clone ID Related Accession (Same CDS sequence)	XM_011446428.2 , XM_011446427.2
Accession Version	XM_011446428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011446428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGG TATTCGGCTG GGGCAGAAGT AATGATGGAC AACTGTCTAT CGGATTTTCG 
GAGGAGACAA GCATTCTCTC CCCTAAGGAA GTGTCTCGCC TCCAGGAGCT GGACATTAAG 
GAGATCAGCT GTGGAGAGAA GCACACTCTC ATTGTCACCA AGGATGGGAA GCTTTACTCG 
TGTGGCAGTA ACGAATATGG AGAACTGGGT CACAGTAAAC CAAGGTCTCG GGCAGAGTTG 
GTGAGTTCTC TGAGCAGTAT GCAGGTCATA CAGGCGGCAG GAGGGTCCGA GTTCAGTCTG 
GTGATTACGA CTGCGGGGGA GGTGTTCAGT TGGGGCAGGA ACGACAAAGG ACAGCTGGGG 
CGAGGAAAAC TCTCTAAGGA AGAGGAAAGG AAACCAAAAC TGATGAAGAG CCTTGCTGTG 
TGTACAGTGG TACAGATAAC ATGCGGGGCC AATCATTGCC TGGCACTCAC CGATGATGGT 
CGTCTGTTTT CTTGGGGTTG TAATAAATAC GGCCAGTTAG GACTTGGCTC AAGTCACTCA 
TTCCATGACA CTCCCCAGCA AATTGTCTGT CTGCGGGGCA TACCTATAGC ACAGATTGCT 
TCGGGGGGTA ACCATAGCTT TATACTGACC TGGTCCAGAG CAGTGTTTGG ATGGGGCAAA 
AACAGTTTTG GACAGCTTGG TTTAAATGAT GAGTATGATA AAAGTCATCC CACTTTATGC 
AAGTCTCTGA GGAACCAGAA GGTCATGTAC ATCTCTTGTG GGGAAGACCA CACTGTGGCC 
TTGACCAAGG AAGGTGGTGT TTTCTCATTT GGGGCAGGGG GTTTTGGGCA GTTGGGACAC 
AACTCCACTC AGAACGAGAT CTTACCTAAA CGGGTGATGG AGCTGATGGG GAGTGTCATA 
TCTCAGGTCG CATGTGGAAG GCGACACACG GTAGCATTTT CTCCGTCCAG TGGTAAGATG 
TACGCATTTG GATCAGGAGG ATCCGGACAG CTTGGATTAG GGGATACCTC AGCTCGTAAC 
TCTCCGTTTC CAATCCTGAG TCCGAATTTT CCCAGCACAG GGCGGCGATT GTCTCAAGTC 
ATGGACCATG ACGGCTCGTT ATACCTGGTC AAGCATCTGT ACAGCGGGGG AGATCATTGC 
CTACTACTGG CCAGGAATGC GGAGATGGGT GGAGAGCCAG ATGATTTCAG GGAGGTTGAC 
CCCAGCACCC AGATAACAAC CCTGACCGCT GAGAAATGTG AAAAGATCAA ATCTCTACAG 
CTGGATGTCA TCACACCATC AGAACTCGTC GATGATGTTT TGAAAATATT TTCAAGTTCT 
GCATGTTTGA ATGCATCATT TTTACTGAAA AATGATGAAC ATTTTGGCTG TAGCAGTAAG 
AATAATGGCG TAGACATGGA CAATGTCAGG AAGCTGATGA ACCGAATCGC TGACGCTAAA 
AATGTGAACA TCATTCAGCA GATAACAGAG AGCATTGAGA ACCGCCTGTT GAGGTCTCTT 
CCTCCCTCAC CCCCCGATGT AGAGGCTCTC AGAGTCTACC TCATTCTGCC AGAATTCCAT 
TTGTTATACG AACCCAAATT CTTCTCAACT CTGATTGGTC CATTTGGGAA GTCTATAGTA 
GGATTAGAAA AGGCAGCATC AAGAGTGCTA GATATATGGT GGGGATCTCT AAAGCCAAGG 
TTTTTTAAAA GGATTTTAGG GATCTTCAAA CAGACAGCAT TATTAATTCT TCGTCTGCCA 
AATCTACCTG CCACTAGCAA TATGGAAGTG ATGATTCGTT TTGAAAGTTT GAAGGCTTCA 
TTAGAGGTTC TGAAAAAATT AAACAATGTC AATGACTATA ATGGTCAAAT AATTCCTTAT 
GACAACTTCT ACATACCAGA GGTTACAGAT ATTGTTGACA TTAGAAAAGA TTACGTCAAC 
TGGGTTCACA GGGGGAAAAC TCTACCATCG CCCCAGCTGC ATTTCTGTGA TTATCCATTT 
ATATTTGATG CGTCTGCTAA AAGTGTTCTT CTACAGACAG ATGCCTGCAT GCAAATGCAG 
TCTGCATACG AAGAAGTTCA GAGACGAAAT TTCCAGAATC TGTTTCTTCC TATTGATCCA 
ATAAACCCCA TGTTGATATT ACACGTGACG CGACATAACA TTGTAACGGA TACGCTTCAA 
CAACTGTCTC GTCAAGGACC AGCTGATCTC AAGAAACCAC TCAAGGTGAT ATTTGTTAAT 
GAGGAGGCCA TCGATGAAGG TGGAGTCAGA AAGGAGTTCT TTCTGCTATT GATGAAAGAA 
ATACTTGATC CCAAATATGG AATGTTTAAA TTCTTTGAGG AATCAAGACT GCAATGGTTC 
AACCCTAACT CTTTTGAGGA CAAACAAATG TTTCATCTGA TTGGTGCTGT TTGTGGACTG 
GCAATATATA ACTCCACCAT CATTGGTCTC AGCTTTCCCC TGGCGCTGTA CAAAAAACTG 
CTCAATAGAA AAACAACATT AGAAGACTTG ATGGAGTTGA TGCCTTCAGT TGGCAGAGGT 
ATGCATGACC TCTTAGATTA TGAAGGAGAT GATGTAGAAG ATGTGTTCTG CTTAACTTTT 
GAGATTACAG TAGAGAGTTT TGGAGAAGTT CAACATAAAC CTCTTGTAGA GGGTGGATCG 
GATAGGCCGG TAACCAAAGA CAACAGACAG GAGTACATAG ATGCCTATGT AGACTATTTA 
TTCAACAAAT CAGTGGAGGA CCATTTCAGT GCTTTCAGCG ATGGCTTCCA CAAAGTTTGT 
GGGGGAAAAG TTCTGGAACT TTTCCATCCA CAAGAACTAC AGGCCATGGT AATAGGAAAT 
GAAAACTATG ATTTCCATGA ATTTGAAAAG AATACGGAAT ACAAGGGAGA GTACTACCGA 
CAACATCCCA CGATCCGACT CTTCTGGGAG GTGTTCCACG AGCTGACCAT TCAGCTCAAA 
AAGAAGTTCT TATTATTTTT GACTGGGAGT GACCGGATTC CAATCCTGGG AATGAAGGCG 
GTGAAGATGA TCATCCAGCC CACCCATGGT GGGAACAGTT ACCTCCCCGT CGCCCACACA 
TGTTTTAATC TGTTAGACCT CCCCAAGTAC CAGGACTACC GGACCATGAA AGAGAAGCTC 
TGTATGGCCA TAGAACAGAC CGAGGGATTC GGGATTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011444730.1
CDS	249..3410
Translation	MIGVFGWGRSNDGQLSIGFSEETSILSPKEVSRLQELDIKEISCGEKHTLIVTKDGKLYSCGSNEYGELGHSKPRSRAELVSSLSSMQVIQAAGGSEFSLVITTAGEVFSWGRNDKGQLGRGKLSKEEERKPKLMKSLAVCTVVQITCGANHCLALTDDGRLFSWGCNKYGQLGLGSSHSFHDTPQQIVCLRGIPIAQIASGGNHSFILTWSRAVFGWGKNSFGQLGLNDEYDKSHPTLCKSLRNQKVMYISCGEDHTVALTKEGGVFSFGAGGFGQLGHNSTQNEILPKRVMELMGSVISQVACGRRHTVAFSPSSGKMYAFGSGGSGQLGLGDTSARNSPFPILSPNFPSTGRRLSQVMDHDGSLYLVKHLYSGGDHCLLLARNAEMGGEPDDFREVDPSTQITTLTAEKCEKIKSLQLDVITPSELVDDVLKIFSSSACLNASFLLKNDEHFGCSSKNNGVDMDNVRKLMNRIADAKNVNIIQQITESIENRLLRSLPPSPPDVEALRVYLILPEFHLLYEPKFFSTLIGPFGKSIVGLEKAASRVLDIWWGSLKPRFFKRILGIFKQTALLILRLPNLPATSNMEVMIRFESLKASLEVLKKLNNVNDYNGQIIPYDNFYIPEVTDIVDIRKDYVNWVHRGKTLPSPQLHFCDYPFIFDASAKSVLLQTDACMQMQSAYEEVQRRNFQNLFLPIDPINPMLILHVTRHNIVTDTLQQLSRQGPADLKKPLKVIFVNEEAIDEGGVRKEFFLLLMKEILDPKYGMFKFFEESRLQWFNPNSFEDKQMFHLIGAVCGLAIYNSTIIGLSFPLALYKKLLNRKTTLEDLMELMPSVGRGMHDLLDYEGDDVEDVFCLTFEITVESFGEVQHKPLVEGGSDRPVTKDNRQEYIDAYVDYLFNKSVEDHFSAFSDGFHKVCGGKVLELFHPQELQAMVIGNENYDFHEFEKNTEYKGEYYRQHPTIRLFWEVFHELTIQLKKKFLLFLTGSDRIPILGMKAVKMIIQPTHGGNSYLPVAHTCFNLLDLPKYQDYRTMKEKLCMAIEQTEGFGIV

Target ORF information:

RefSeq Version	XM_011446428.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011446428.2

ORF Insert Sequence:

ATGATAGGGG TATTCGGCTG GGGCAGAAGT AATGATGGAC AACTGTCTAT CGGATTTTCG 
GAGGAGACAA GCATTCTCTC CCCTAAGGAA GTGTCTCGCC TCCAGGAGCT GGACATTAAG 
GAGATCAGCT GTGGAGAGAA GCACACTCTC ATTGTCACCA AGGATGGGAA GCTTTACTCG 
TGTGGCAGTA ACGAATATGG AGAACTGGGT CACAGTAAAC CAAGGTCTCG GGCAGAGTTG 
GTGAGTTCTC TGAGCAGTAT GCAGGTCATA CAGGCGGCAG GAGGGTCCGA GTTCAGTCTG 
GTGATTACGA CTGCGGGGGA GGTGTTCAGT TGGGGCAGGA ACGACAAAGG ACAGCTGGGG 
CGAGGAAAAC TCTCTAAGGA AGAGGAAAGG AAACCAAAAC TGATGAAGAG CCTTGCTGTG 
TGTACAGTGG TACAGATAAC ATGCGGGGCC AATCATTGCC TGGCACTCAC CGATGATGGT 
CGTCTGTTTT CTTGGGGTTG TAATAAATAC GGCCAGTTAG GACTTGGCTC AAGTCACTCA 
TTCCATGACA CTCCCCAGCA AATTGTCTGT CTGCGGGGCA TACCTATAGC ACAGATTGCT 
TCGGGGGGTA ACCATAGCTT TATACTGACC TGGTCCAGAG CAGTGTTTGG ATGGGGCAAA 
AACAGTTTTG GACAGCTTGG TTTAAATGAT GAGTATGATA AAAGTCATCC CACTTTATGC 
AAGTCTCTGA GGAACCAGAA GGTCATGTAC ATCTCTTGTG GGGAAGACCA CACTGTGGCC 
TTGACCAAGG AAGGTGGTGT TTTCTCATTT GGGGCAGGGG GTTTTGGGCA GTTGGGACAC 
AACTCCACTC AGAACGAGAT CTTACCTAAA CGGGTGATGG AGCTGATGGG GAGTGTCATA 
TCTCAGGTCG CATGTGGAAG GCGACACACG GTAGCATTTT CTCCGTCCAG TGGTAAGATG 
TACGCATTTG GATCAGGAGG ATCCGGACAG CTTGGATTAG GGGATACCTC AGCTCGTAAC 
TCTCCGTTTC CAATCCTGAG TCCGAATTTT CCCAGCACAG GGCGGCGATT GTCTCAAGTC 
ATGGACCATG ACGGCTCGTT ATACCTGGTC AAGCATCTGT ACAGCGGGGG AGATCATTGC 
CTACTACTGG CCAGGAATGC GGAGATGGGT GGAGAGCCAG ATGATTTCAG GGAGGTTGAC 
CCCAGCACCC AGATAACAAC CCTGACCGCT GAGAAATGTG AAAAGATCAA ATCTCTACAG 
CTGGATGTCA TCACACCATC AGAACTCGTC GATGATGTTT TGAAAATATT TTCAAGTTCT 
GCATGTTTGA ATGCATCATT TTTACTGAAA AATGATGAAC ATTTTGGCTG TAGCAGTAAG 
AATAATGGCG TAGACATGGA CAATGTCAGG AAGCTGATGA ACCGAATCGC TGACGCTAAA 
AATGTGAACA TCATTCAGCA GATAACAGAG AGCATTGAGA ACCGCCTGTT GAGGTCTCTT 
CCTCCCTCAC CCCCCGATGT AGAGGCTCTC AGAGTCTACC TCATTCTGCC AGAATTCCAT 
TTGTTATACG AACCCAAATT CTTCTCAACT CTGATTGGTC CATTTGGGAA GTCTATAGTA 
GGATTAGAAA AGGCAGCATC AAGAGTGCTA GATATATGGT GGGGATCTCT AAAGCCAAGG 
TTTTTTAAAA GGATTTTAGG GATCTTCAAA CAGACAGCAT TATTAATTCT TCGTCTGCCA 
AATCTACCTG CCACTAGCAA TATGGAAGTG ATGATTCGTT TTGAAAGTTT GAAGGCTTCA 
TTAGAGGTTC TGAAAAAATT AAACAATGTC AATGACTATA ATGGTCAAAT AATTCCTTAT 
GACAACTTCT ACATACCAGA GGTTACAGAT ATTGTTGACA TTAGAAAAGA TTACGTCAAC 
TGGGTTCACA GGGGGAAAAC TCTACCATCG CCCCAGCTGC ATTTCTGTGA TTATCCATTT 
ATATTTGATG CGTCTGCTAA AAGTGTTCTT CTACAGACAG ATGCCTGCAT GCAAATGCAG 
TCTGCATACG AAGAAGTTCA GAGACGAAAT TTCCAGAATC TGTTTCTTCC TATTGATCCA 
ATAAACCCCA TGTTGATATT ACACGTGACG CGACATAACA TTGTAACGGA TACGCTTCAA 
CAACTGTCTC GTCAAGGACC AGCTGATCTC AAGAAACCAC TCAAGGTGAT ATTTGTTAAT 
GAGGAGGCCA TCGATGAAGG TGGAGTCAGA AAGGAGTTCT TTCTGCTATT GATGAAAGAA 
ATACTTGATC CCAAATATGG AATGTTTAAA TTCTTTGAGG AATCAAGACT GCAATGGTTC 
AACCCTAACT CTTTTGAGGA CAAACAAATG TTTCATCTGA TTGGTGCTGT TTGTGGACTG 
GCAATATATA ACTCCACCAT CATTGGTCTC AGCTTTCCCC TGGCGCTGTA CAAAAAACTG 
CTCAATAGAA AAACAACATT AGAAGACTTG ATGGAGTTGA TGCCTTCAGT TGGCAGAGGT 
ATGCATGACC TCTTAGATTA TGAAGGAGAT GATGTAGAAG ATGTGTTCTG CTTAACTTTT 
GAGATTACAG TAGAGAGTTT TGGAGAAGTT CAACATAAAC CTCTTGTAGA GGGTGGATCG 
GATAGGCCGG TAACCAAAGA CAACAGACAG GAGTACATAG ATGCCTATGT AGACTATTTA 
TTCAACAAAT CAGTGGAGGA CCATTTCAGT GCTTTCAGCG ATGGCTTCCA CAAAGTTTGT 
GGGGGAAAAG TTCTGGAACT TTTCCATCCA CAAGAACTAC AGGCCATGGT AATAGGAAAT 
GAAAACTATG ATTTCCATGA ATTTGAAAAG AATACGGAAT ACAAGGGAGA GTACTACCGA 
CAACATCCCA CGATCCGACT CTTCTGGGAG GTGTTCCACG AGCTGACCAT TCAGCTCAAA 
AAGAAGTTCT TATTATTTTT GACTGGGAGT GACCGGATTC CAATCCTGGG AATGAAGGCG 
GTGAAGATGA TCATCCAGCC CACCCATGGT GGGAACAGTT ACCTCCCCGT CGCCCACACA 
TGTTTTAATC TGTTAGACCT CCCCAAGTAC CAGGACTACC GGACCATGAA AGAGAAGCTC 
TGTATGGCCA TAGAACAGAC CGAGGGATTC GGGATTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105340402 ( XM_011446427.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011444729.1 Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr106953
Clone ID Related Accession (Same CDS sequence)	XM_011446428.2 , XM_011446427.2
Accession Version	XM_011446427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011446427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGGGG TATTCGGCTG GGGCAGAAGT AATGATGGAC AACTGTCTAT CGGATTTTCG 
GAGGAGACAA GCATTCTCTC CCCTAAGGAA GTGTCTCGCC TCCAGGAGCT GGACATTAAG 
GAGATCAGCT GTGGAGAGAA GCACACTCTC ATTGTCACCA AGGATGGGAA GCTTTACTCG 
TGTGGCAGTA ACGAATATGG AGAACTGGGT CACAGTAAAC CAAGGTCTCG GGCAGAGTTG 
GTGAGTTCTC TGAGCAGTAT GCAGGTCATA CAGGCGGCAG GAGGGTCCGA GTTCAGTCTG 
GTGATTACGA CTGCGGGGGA GGTGTTCAGT TGGGGCAGGA ACGACAAAGG ACAGCTGGGG 
CGAGGAAAAC TCTCTAAGGA AGAGGAAAGG AAACCAAAAC TGATGAAGAG CCTTGCTGTG 
TGTACAGTGG TACAGATAAC ATGCGGGGCC AATCATTGCC TGGCACTCAC CGATGATGGT 
CGTCTGTTTT CTTGGGGTTG TAATAAATAC GGCCAGTTAG GACTTGGCTC AAGTCACTCA 
TTCCATGACA CTCCCCAGCA AATTGTCTGT CTGCGGGGCA TACCTATAGC ACAGATTGCT 
TCGGGGGGTA ACCATAGCTT TATACTGACC TGGTCCAGAG CAGTGTTTGG ATGGGGCAAA 
AACAGTTTTG GACAGCTTGG TTTAAATGAT GAGTATGATA AAAGTCATCC CACTTTATGC 
AAGTCTCTGA GGAACCAGAA GGTCATGTAC ATCTCTTGTG GGGAAGACCA CACTGTGGCC 
TTGACCAAGG AAGGTGGTGT TTTCTCATTT GGGGCAGGGG GTTTTGGGCA GTTGGGACAC 
AACTCCACTC AGAACGAGAT CTTACCTAAA CGGGTGATGG AGCTGATGGG GAGTGTCATA 
TCTCAGGTCG CATGTGGAAG GCGACACACG GTAGCATTTT CTCCGTCCAG TGGTAAGATG 
TACGCATTTG GATCAGGAGG ATCCGGACAG CTTGGATTAG GGGATACCTC AGCTCGTAAC 
TCTCCGTTTC CAATCCTGAG TCCGAATTTT CCCAGCACAG GGCGGCGATT GTCTCAAGTC 
ATGGACCATG ACGGCTCGTT ATACCTGGTC AAGCATCTGT ACAGCGGGGG AGATCATTGC 
CTACTACTGG CCAGGAATGC GGAGATGGGT GGAGAGCCAG ATGATTTCAG GGAGGTTGAC 
CCCAGCACCC AGATAACAAC CCTGACCGCT GAGAAATGTG AAAAGATCAA ATCTCTACAG 
CTGGATGTCA TCACACCATC AGAACTCGTC GATGATGTTT TGAAAATATT TTCAAGTTCT 
GCATGTTTGA ATGCATCATT TTTACTGAAA AATGATGAAC ATTTTGGCTG TAGCAGTAAG 
AATAATGGCG TAGACATGGA CAATGTCAGG AAGCTGATGA ACCGAATCGC TGACGCTAAA 
AATGTGAACA TCATTCAGCA GATAACAGAG AGCATTGAGA ACCGCCTGTT GAGGTCTCTT 
CCTCCCTCAC CCCCCGATGT AGAGGCTCTC AGAGTCTACC TCATTCTGCC AGAATTCCAT 
TTGTTATACG AACCCAAATT CTTCTCAACT CTGATTGGTC CATTTGGGAA GTCTATAGTA 
GGATTAGAAA AGGCAGCATC AAGAGTGCTA GATATATGGT GGGGATCTCT AAAGCCAAGG 
TTTTTTAAAA GGATTTTAGG GATCTTCAAA CAGACAGCAT TATTAATTCT TCGTCTGCCA 
AATCTACCTG CCACTAGCAA TATGGAAGTG ATGATTCGTT TTGAAAGTTT GAAGGCTTCA 
TTAGAGGTTC TGAAAAAATT AAACAATGTC AATGACTATA ATGGTCAAAT AATTCCTTAT 
GACAACTTCT ACATACCAGA GGTTACAGAT ATTGTTGACA TTAGAAAAGA TTACGTCAAC 
TGGGTTCACA GGGGGAAAAC TCTACCATCG CCCCAGCTGC ATTTCTGTGA TTATCCATTT 
ATATTTGATG CGTCTGCTAA AAGTGTTCTT CTACAGACAG ATGCCTGCAT GCAAATGCAG 
TCTGCATACG AAGAAGTTCA GAGACGAAAT TTCCAGAATC TGTTTCTTCC TATTGATCCA 
ATAAACCCCA TGTTGATATT ACACGTGACG CGACATAACA TTGTAACGGA TACGCTTCAA 
CAACTGTCTC GTCAAGGACC AGCTGATCTC AAGAAACCAC TCAAGGTGAT ATTTGTTAAT 
GAGGAGGCCA TCGATGAAGG TGGAGTCAGA AAGGAGTTCT TTCTGCTATT GATGAAAGAA 
ATACTTGATC CCAAATATGG AATGTTTAAA TTCTTTGAGG AATCAAGACT GCAATGGTTC 
AACCCTAACT CTTTTGAGGA CAAACAAATG TTTCATCTGA TTGGTGCTGT TTGTGGACTG 
GCAATATATA ACTCCACCAT CATTGGTCTC AGCTTTCCCC TGGCGCTGTA CAAAAAACTG 
CTCAATAGAA AAACAACATT AGAAGACTTG ATGGAGTTGA TGCCTTCAGT TGGCAGAGGT 
ATGCATGACC TCTTAGATTA TGAAGGAGAT GATGTAGAAG ATGTGTTCTG CTTAACTTTT 
GAGATTACAG TAGAGAGTTT TGGAGAAGTT CAACATAAAC CTCTTGTAGA GGGTGGATCG 
GATAGGCCGG TAACCAAAGA CAACAGACAG GAGTACATAG ATGCCTATGT AGACTATTTA 
TTCAACAAAT CAGTGGAGGA CCATTTCAGT GCTTTCAGCG ATGGCTTCCA CAAAGTTTGT 
GGGGGAAAAG TTCTGGAACT TTTCCATCCA CAAGAACTAC AGGCCATGGT AATAGGAAAT 
GAAAACTATG ATTTCCATGA ATTTGAAAAG AATACGGAAT ACAAGGGAGA GTACTACCGA 
CAACATCCCA CGATCCGACT CTTCTGGGAG GTGTTCCACG AGCTGACCAT TCAGCTCAAA 
AAGAAGTTCT TATTATTTTT GACTGGGAGT GACCGGATTC CAATCCTGGG AATGAAGGCG 
GTGAAGATGA TCATCCAGCC CACCCATGGT GGGAACAGTT ACCTCCCCGT CGCCCACACA 
TGTTTTAATC TGTTAGACCT CCCCAAGTAC CAGGACTACC GGACCATGAA AGAGAAGCTC 
TGTATGGCCA TAGAACAGAC CGAGGGATTC GGGATTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011444729.1
CDS	253..3414
Translation	MIGVFGWGRSNDGQLSIGFSEETSILSPKEVSRLQELDIKEISCGEKHTLIVTKDGKLYSCGSNEYGELGHSKPRSRAELVSSLSSMQVIQAAGGSEFSLVITTAGEVFSWGRNDKGQLGRGKLSKEEERKPKLMKSLAVCTVVQITCGANHCLALTDDGRLFSWGCNKYGQLGLGSSHSFHDTPQQIVCLRGIPIAQIASGGNHSFILTWSRAVFGWGKNSFGQLGLNDEYDKSHPTLCKSLRNQKVMYISCGEDHTVALTKEGGVFSFGAGGFGQLGHNSTQNEILPKRVMELMGSVISQVACGRRHTVAFSPSSGKMYAFGSGGSGQLGLGDTSARNSPFPILSPNFPSTGRRLSQVMDHDGSLYLVKHLYSGGDHCLLLARNAEMGGEPDDFREVDPSTQITTLTAEKCEKIKSLQLDVITPSELVDDVLKIFSSSACLNASFLLKNDEHFGCSSKNNGVDMDNVRKLMNRIADAKNVNIIQQITESIENRLLRSLPPSPPDVEALRVYLILPEFHLLYEPKFFSTLIGPFGKSIVGLEKAASRVLDIWWGSLKPRFFKRILGIFKQTALLILRLPNLPATSNMEVMIRFESLKASLEVLKKLNNVNDYNGQIIPYDNFYIPEVTDIVDIRKDYVNWVHRGKTLPSPQLHFCDYPFIFDASAKSVLLQTDACMQMQSAYEEVQRRNFQNLFLPIDPINPMLILHVTRHNIVTDTLQQLSRQGPADLKKPLKVIFVNEEAIDEGGVRKEFFLLLMKEILDPKYGMFKFFEESRLQWFNPNSFEDKQMFHLIGAVCGLAIYNSTIIGLSFPLALYKKLLNRKTTLEDLMELMPSVGRGMHDLLDYEGDDVEDVFCLTFEITVESFGEVQHKPLVEGGSDRPVTKDNRQEYIDAYVDYLFNKSVEDHFSAFSDGFHKVCGGKVLELFHPQELQAMVIGNENYDFHEFEKNTEYKGEYYRQHPTIRLFWEVFHELTIQLKKKFLLFLTGSDRIPILGMKAVKMIIQPTHGGNSYLPVAHTCFNLLDLPKYQDYRTMKEKLCMAIEQTEGFGIV

Target ORF information:

RefSeq Version	XM_011446427.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas probable E3 ubiquitin-protein ligase HERC4 (LOC105340402), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011446427.2

ORF Insert Sequence:

ATGATAGGGG TATTCGGCTG GGGCAGAAGT AATGATGGAC AACTGTCTAT CGGATTTTCG 
GAGGAGACAA GCATTCTCTC CCCTAAGGAA GTGTCTCGCC TCCAGGAGCT GGACATTAAG 
GAGATCAGCT GTGGAGAGAA GCACACTCTC ATTGTCACCA AGGATGGGAA GCTTTACTCG 
TGTGGCAGTA ACGAATATGG AGAACTGGGT CACAGTAAAC CAAGGTCTCG GGCAGAGTTG 
GTGAGTTCTC TGAGCAGTAT GCAGGTCATA CAGGCGGCAG GAGGGTCCGA GTTCAGTCTG 
GTGATTACGA CTGCGGGGGA GGTGTTCAGT TGGGGCAGGA ACGACAAAGG ACAGCTGGGG 
CGAGGAAAAC TCTCTAAGGA AGAGGAAAGG AAACCAAAAC TGATGAAGAG CCTTGCTGTG 
TGTACAGTGG TACAGATAAC ATGCGGGGCC AATCATTGCC TGGCACTCAC CGATGATGGT 
CGTCTGTTTT CTTGGGGTTG TAATAAATAC GGCCAGTTAG GACTTGGCTC AAGTCACTCA 
TTCCATGACA CTCCCCAGCA AATTGTCTGT CTGCGGGGCA TACCTATAGC ACAGATTGCT 
TCGGGGGGTA ACCATAGCTT TATACTGACC TGGTCCAGAG CAGTGTTTGG ATGGGGCAAA 
AACAGTTTTG GACAGCTTGG TTTAAATGAT GAGTATGATA AAAGTCATCC CACTTTATGC 
AAGTCTCTGA GGAACCAGAA GGTCATGTAC ATCTCTTGTG GGGAAGACCA CACTGTGGCC 
TTGACCAAGG AAGGTGGTGT TTTCTCATTT GGGGCAGGGG GTTTTGGGCA GTTGGGACAC 
AACTCCACTC AGAACGAGAT CTTACCTAAA CGGGTGATGG AGCTGATGGG GAGTGTCATA 
TCTCAGGTCG CATGTGGAAG GCGACACACG GTAGCATTTT CTCCGTCCAG TGGTAAGATG 
TACGCATTTG GATCAGGAGG ATCCGGACAG CTTGGATTAG GGGATACCTC AGCTCGTAAC 
TCTCCGTTTC CAATCCTGAG TCCGAATTTT CCCAGCACAG GGCGGCGATT GTCTCAAGTC 
ATGGACCATG ACGGCTCGTT ATACCTGGTC AAGCATCTGT ACAGCGGGGG AGATCATTGC 
CTACTACTGG CCAGGAATGC GGAGATGGGT GGAGAGCCAG ATGATTTCAG GGAGGTTGAC 
CCCAGCACCC AGATAACAAC CCTGACCGCT GAGAAATGTG AAAAGATCAA ATCTCTACAG 
CTGGATGTCA TCACACCATC AGAACTCGTC GATGATGTTT TGAAAATATT TTCAAGTTCT 
GCATGTTTGA ATGCATCATT TTTACTGAAA AATGATGAAC ATTTTGGCTG TAGCAGTAAG 
AATAATGGCG TAGACATGGA CAATGTCAGG AAGCTGATGA ACCGAATCGC TGACGCTAAA 
AATGTGAACA TCATTCAGCA GATAACAGAG AGCATTGAGA ACCGCCTGTT GAGGTCTCTT 
CCTCCCTCAC CCCCCGATGT AGAGGCTCTC AGAGTCTACC TCATTCTGCC AGAATTCCAT 
TTGTTATACG AACCCAAATT CTTCTCAACT CTGATTGGTC CATTTGGGAA GTCTATAGTA 
GGATTAGAAA AGGCAGCATC AAGAGTGCTA GATATATGGT GGGGATCTCT AAAGCCAAGG 
TTTTTTAAAA GGATTTTAGG GATCTTCAAA CAGACAGCAT TATTAATTCT TCGTCTGCCA 
AATCTACCTG CCACTAGCAA TATGGAAGTG ATGATTCGTT TTGAAAGTTT GAAGGCTTCA 
TTAGAGGTTC TGAAAAAATT AAACAATGTC AATGACTATA ATGGTCAAAT AATTCCTTAT 
GACAACTTCT ACATACCAGA GGTTACAGAT ATTGTTGACA TTAGAAAAGA TTACGTCAAC 
TGGGTTCACA GGGGGAAAAC TCTACCATCG CCCCAGCTGC ATTTCTGTGA TTATCCATTT 
ATATTTGATG CGTCTGCTAA AAGTGTTCTT CTACAGACAG ATGCCTGCAT GCAAATGCAG 
TCTGCATACG AAGAAGTTCA GAGACGAAAT TTCCAGAATC TGTTTCTTCC TATTGATCCA 
ATAAACCCCA TGTTGATATT ACACGTGACG CGACATAACA TTGTAACGGA TACGCTTCAA 
CAACTGTCTC GTCAAGGACC AGCTGATCTC AAGAAACCAC TCAAGGTGAT ATTTGTTAAT 
GAGGAGGCCA TCGATGAAGG TGGAGTCAGA AAGGAGTTCT TTCTGCTATT GATGAAAGAA 
ATACTTGATC CCAAATATGG AATGTTTAAA TTCTTTGAGG AATCAAGACT GCAATGGTTC 
AACCCTAACT CTTTTGAGGA CAAACAAATG TTTCATCTGA TTGGTGCTGT TTGTGGACTG 
GCAATATATA ACTCCACCAT CATTGGTCTC AGCTTTCCCC TGGCGCTGTA CAAAAAACTG 
CTCAATAGAA AAACAACATT AGAAGACTTG ATGGAGTTGA TGCCTTCAGT TGGCAGAGGT 
ATGCATGACC TCTTAGATTA TGAAGGAGAT GATGTAGAAG ATGTGTTCTG CTTAACTTTT 
GAGATTACAG TAGAGAGTTT TGGAGAAGTT CAACATAAAC CTCTTGTAGA GGGTGGATCG 
GATAGGCCGG TAACCAAAGA CAACAGACAG GAGTACATAG ATGCCTATGT AGACTATTTA 
TTCAACAAAT CAGTGGAGGA CCATTTCAGT GCTTTCAGCG ATGGCTTCCA CAAAGTTTGT 
GGGGGAAAAG TTCTGGAACT TTTCCATCCA CAAGAACTAC AGGCCATGGT AATAGGAAAT 
GAAAACTATG ATTTCCATGA ATTTGAAAAG AATACGGAAT ACAAGGGAGA GTACTACCGA 
CAACATCCCA CGATCCGACT CTTCTGGGAG GTGTTCCACG AGCTGACCAT TCAGCTCAAA 
AAGAAGTTCT TATTATTTTT GACTGGGAGT GACCGGATTC CAATCCTGGG AATGAAGGCG 
GTGAAGATGA TCATCCAGCC CACCCATGGT GGGAACAGTT ACCTCCCCGT CGCCCACACA 
TGTTTTAATC TGTTAGACCT CCCCAAGTAC CAGGACTACC GGACCATGAA AGAGAAGCTC 
TGTATGGCCA TAGAACAGAC CGAGGGATTC GGGATTGTGT AA