LOC105327779 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105327779
Entrez Gene ID	105327779
Full Name	3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011428405.2	XP_011426707.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
XM_020067038.1	XP_019922597.1	3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105327779 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105327779 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr92564	XM_020067038.1 Latest version!	Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCr92565	XM_011428405.2 Latest version!	Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105327779 ( XM_020067038.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_019922597.1 Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr92564
Clone ID Related Accession (Same CDS sequence)	XM_020067038.1
Accession Version	XM_020067038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGA GTACAGGTTT ACCATCATTT GTTAAAATCG TAGAAGTGGG ACCCAGAGAT 
GGACTTCAGA ATGAAAAGAT GTTTGTGCCA ACAGAAACCA AAGTGGAATT TATCAATAAG 
CTGTCTGATG CAGGACTGTC AGTCATTGAA GTTACAAGTT TTGTGTCTCC AAAATGGGTC 
CCACAGATGG CAGACAATGC AGAAGTTCTG AAATTGATCA AGAGGAGAGA TGGAGTCACT 
TATGCAGCTC TCACTCCAAA TCTGAAAGGC CTCAAAGATG CTATTTCTTG TGGTGTAAAT 
GAAGTTGCAA TTTTTGGAGC CGCATCTGAA ACATTTAGCA AGAAAAATAT AAATTGCAGT 
ATATCAGAGA GTTTACAGAG ATTTGAAGAA GTTGTCAAAT TAGCTAAGGA AATTAAACTG 
CCTGTTAGAG GGTATGTCTC GTGTGTTCTT GGATGTCCTT ATGAAAAAAT TGTCTCACCA 
GAGAAGGTTG CACAGGTTGC CAAGCAGATG TTTGAGATGG GTTGCTATGA AATATCCCTT 
GGTGACACCA TTGGTGTAGG AACTCCAGGA GCTATGAAAC AGCTGATTGA CGTTGTAACG 
AAACATGTCC CAATTGAAAA AGTGGCTGTT CATTGTCATG ACACCTATGG ACAGGCTCTG 
GCTAATATCC TGGCAGCATT ACAGATGGGC GTGTCTGTCG TGGATAGTTC TACCGCGGGT 
CTTGGCGGTT GTCCATATGC CAAGGGAGCT AGTGGCAATG TGGCAACAGA AGATGTACTG 
TACATGCTGG ATGGGCTTGG AATCAAAACA GGAGTTGATC TGCAGAAGAC AGTGGAAGCT 
GGTCGATTTA TTTCACAAGC ATTAGGACGA AGTACACACT CTAAAGTGGG ACAAGCCATG 
AAATCCTCAT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019922597.1
CDS	276..1190
Translation	MIKSTGLPSFVKIVEVGPRDGLQNEKMFVPTETKVEFINKLSDAGLSVIEVTSFVSPKWVPQMADNAEVLKLIKRRDGVTYAALTPNLKGLKDAISCGVNEVAIFGAASETFSKKNINCSISESLQRFEEVVKLAKEIKLPVRGYVSCVLGCPYEKIVSPEKVAQVAKQMFEMGCYEISLGDTIGVGTPGAMKQLIDVVTKHVPIEKVAVHCHDTYGQALANILAALQMGVSVVDSSTAGLGGCPYAKGASGNVATEDVLYMLDGLGIKTGVDLQKTVEAGRFISQALGRSTHSKVGQAMKSSL

Target ORF information:

RefSeq Version	XM_020067038.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020067038.1

ORF Insert Sequence:

ATGATAAAGA GTACAGGTTT ACCATCATTT GTTAAAATCG TAGAAGTGGG ACCCAGAGAT 
GGACTTCAGA ATGAAAAGAT GTTTGTGCCA ACAGAAACCA AAGTGGAATT TATCAATAAG 
CTGTCTGATG CAGGACTGTC AGTCATTGAA GTTACAAGTT TTGTGTCTCC AAAATGGGTC 
CCACAGATGG CAGACAATGC AGAAGTTCTG AAATTGATCA AGAGGAGAGA TGGAGTCACT 
TATGCAGCTC TCACTCCAAA TCTGAAAGGC CTCAAAGATG CTATTTCTTG TGGTGTAAAT 
GAAGTTGCAA TTTTTGGAGC CGCATCTGAA ACATTTAGCA AGAAAAATAT AAATTGCAGT 
ATATCAGAGA GTTTACAGAG ATTTGAAGAA GTTGTCAAAT TAGCTAAGGA AATTAAACTG 
CCTGTTAGAG GGTATGTCTC GTGTGTTCTT GGATGTCCTT ATGAAAAAAT TGTCTCACCA 
GAGAAGGTTG CACAGGTTGC CAAGCAGATG TTTGAGATGG GTTGCTATGA AATATCCCTT 
GGTGACACCA TTGGTGTAGG AACTCCAGGA GCTATGAAAC AGCTGATTGA CGTTGTAACG 
AAACATGTCC CAATTGAAAA AGTGGCTGTT CATTGTCATG ACACCTATGG ACAGGCTCTG 
GCTAATATCC TGGCAGCATT ACAGATGGGC GTGTCTGTCG TGGATAGTTC TACCGCGGGT 
CTTGGCGGTT GTCCATATGC CAAGGGAGCT AGTGGCAATG TGGCAACAGA AGATGTACTG 
TACATGCTGG ATGGGCTTGG AATCAAAACA GGAGTTGATC TGCAGAAGAC AGTGGAAGCT 
GGTCGATTTA TTTCACAAGC ATTAGGACGA AGTACACACT CTAAAGTGGG ACAAGCCATG 
AAATCCTCAT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105327779 ( XM_011428405.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011426707.1 Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr92565
Clone ID Related Accession (Same CDS sequence)	XM_011428405.2
Accession Version	XM_011428405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011428405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCCA CAGGTTTGTG TCTTAATTTT CAGATGGCAG ACAATGCAGA AGTTCTGAAA 
TTGATCAAGA GGAGAGATGG AGTCACTTAT GCAGCTCTCA CTCCAAATCT GAAAGGCCTC 
AAAGATGCTA TTTCTTGTGG TGTAAATGAA GTTGCAATTT TTGGAGCCGC ATCTGAAACA 
TTTAGCAAGA AAAATATAAA TTGCAGTATA TCAGAGAGTT TACAGAGATT TGAAGAAGTT 
GTCAAATTAG CTAAGGAAAT TAAACTGCCT GTTAGAGGGT ATGTCTCGTG TGTTCTTGGA 
TGTCCTTATG AAAAAATTGT CTCACCAGAG AAGGTTGCAC AGGTTGCCAA GCAGATGTTT 
GAGATGGGTT GCTATGAAAT ATCCCTTGGT GACACCATTG GTGTAGGAAC TCCAGGAGCT 
ATGAAACAGC TGATTGACGT TGTAACGAAA CATGTCCCAA TTGAAAAAGT GGCTGTTCAT 
TGTCATGACA CCTATGGACA GGCTCTGGCT AATATCCTGG CAGCATTACA GATGGGCGTG 
TCTGTCGTGG ATAGTTCTAC CGCGGGTCTT GGCGGTTGTC CATATGCCAA GGGAGCTAGT 
GGCAATGTGG CAACAGAAGA TGTACTGTAC ATGCTGGATG GGCTTGGAAT CAAAACAGGA 
GTTGATCTGC AGAAGACAGT GGAAGCTGGT CGATTTATTT CACAAGCATT AGGACGAAGT 
ACACACTCTA AAGTGGGACA AGCCATGAAA TCCTCATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011426707.1
CDS	254..1015
Translation	MGPTGLCLNFQMADNAEVLKLIKRRDGVTYAALTPNLKGLKDAISCGVNEVAIFGAASETFSKKNINCSISESLQRFEEVVKLAKEIKLPVRGYVSCVLGCPYEKIVSPEKVAQVAKQMFEMGCYEISLGDTIGVGTPGAMKQLIDVVTKHVPIEKVAVHCHDTYGQALANILAALQMGVSVVDSSTAGLGGCPYAKGASGNVATEDVLYMLDGLGIKTGVDLQKTVEAGRFISQALGRSTHSKVGQAMKSSL

Target ORF information:

RefSeq Version	XM_011428405.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas 3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic (LOC105327779), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011428405.2

ORF Insert Sequence:

ATGGGTCCCA CAGGTTTGTG TCTTAATTTT CAGATGGCAG ACAATGCAGA AGTTCTGAAA 
TTGATCAAGA GGAGAGATGG AGTCACTTAT GCAGCTCTCA CTCCAAATCT GAAAGGCCTC 
AAAGATGCTA TTTCTTGTGG TGTAAATGAA GTTGCAATTT TTGGAGCCGC ATCTGAAACA 
TTTAGCAAGA AAAATATAAA TTGCAGTATA TCAGAGAGTT TACAGAGATT TGAAGAAGTT 
GTCAAATTAG CTAAGGAAAT TAAACTGCCT GTTAGAGGGT ATGTCTCGTG TGTTCTTGGA 
TGTCCTTATG AAAAAATTGT CTCACCAGAG AAGGTTGCAC AGGTTGCCAA GCAGATGTTT 
GAGATGGGTT GCTATGAAAT ATCCCTTGGT GACACCATTG GTGTAGGAAC TCCAGGAGCT 
ATGAAACAGC TGATTGACGT TGTAACGAAA CATGTCCCAA TTGAAAAAGT GGCTGTTCAT 
TGTCATGACA CCTATGGACA GGCTCTGGCT AATATCCTGG CAGCATTACA GATGGGCGTG 
TCTGTCGTGG ATAGTTCTAC CGCGGGTCTT GGCGGTTGTC CATATGCCAA GGGAGCTAGT 
GGCAATGTGG CAACAGAAGA TGTACTGTAC ATGCTGGATG GGCTTGGAAT CAAAACAGGA 
GTTGATCTGC AGAAGACAGT GGAAGCTGGT CGATTTATTT CACAAGCATT AGGACGAAGT 
ACACACTCTA AAGTGGGACA AGCCATGAAA TCCTCATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.