LOC105326709 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105326709
Entrez Gene ID	105326709
Full Name	collagen alpha-1(I) chain
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011426881.2	XP_011425183.1	collagen alpha-1(I) chain isoform X2
XM_020066562.1	XP_019922121.1	collagen alpha-1(I) chain isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105326709 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105326709 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr91264	XM_020066562.1 Latest version!	Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCr91265	XM_011426881.2 Latest version!	Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105326709 ( XM_020066562.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_019922121.1 Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr91264
Clone ID Related Accession (Same CDS sequence)	XM_020066562.1
Accession Version	XM_020066562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	collagen alpha-1(I) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCAA GATTACGATC CGCACACCCT GGGTTGCTTA TATCAGCTAT ATTTATTCTA 
TACATTCCAA CTTCTTACTG CTCAAAATGT GATTACACGG TATACGGAAA GGATGACGCA 
CCAGAGTATG ATTTCATATC CTCCTTCAAC AACTTTAAGC GAGTGATCGG GGTGAGGATT 
GACAGGAGGA GCGGTACCCA GGTGGGACAG ACATCCTACG AGTTCAGGAG GAGAGCGGAG 
CGGGGGGACT TGATCATTCC AACAAGAAAT TTGTTTTCGG ACGGGTTTCC GTCGGAGTTC 
TCTCTATCGG TTACCTTGAA GCTGGCCAGT GTAACGAGGC GCGAGTCATG GAACCTGATC 
GACATCACGA ACCCCCAGGG CGAGACAGAC TTCGCTGTGC GCTTTGTTGG GGAGTTACGA 
CAAATTCATC TCTTGTACAG GGACAAGGTT CTCAATACGC TAACAACGGT CGAATTCGAC 
AAAAAAGACA CGCGAAATGT GTTTACAAAG GGATGGCACA AGATGCAGAT TAGCGTTCAA 
AAGACTGAGG CCTCTCTATA TATTGACTGC ACAAGGATAC AGACCGTCCA GATGACGCCA 
TCTGATGTCA GCAACATAGG GTACATGACC GTCGCAAAAT CAATGACTTC CAAACACACC 
ACACCAAAGT TTTACCTGCA GTGGCTAGCT ATGCACTGTG ATCCCACCAA GCCCTCCAGG 
GACAGCTGCG AGGAGTTGGA GGATATTGAC GACAGCGATT CTCAAACATA CAAGGATATC 
ATGTTGGCAG ACAAGCAACA ACGTGGAGGA TCCAAATGTC CCACCCAATG TCCGCCCGGA 
AAAAACGGAA TCCCAGGAAA ACAAGGAGTT TCCGGTCTTC CCGGCAAACC AGGAACCCCC 
GGACAAGATG GACTTCCGGG AGAAAGAGGA CCGGCAGGAA AAGACGGAAA CGACGGACGA 
CCTGGAGAGC CCGGTAAACC CGGACTGCTG GGATTTCAGG GTCAGCCCGG AGAGAGGGGA 
GAACGAGGCT TGAATGGAAA GGACGGAAGC GCCGGAGAGC CTGGCAAGCC AGGAAAAGAT 
GGAAGTGTTG GACCAAGAGG ACTGCAGGGA GAAGAGGGAC CACGGGGAGA GAGGGGAGAA 
ACTGGTTCCC CCGGCCCTGC CGGACCCCCC GGAGAAAGCG TTCAAGGACC TGAAGGAGTT 
GAAGGTCCCC AGGGACCCCC AGGTCAACCA GGACCTCTGG GACCACAGGG AGTTCCCGGG 
CAACAAGGCA ATCCCGGCAA GGACGGTAAA CCAGGAGAGA GGGGGGAGAA GGGAGACAGC 
GGTATGCCTG GTCTACCGGG GGCACAAGGG GCCCAAGGTT TGCAGGGTGT TCCAGGACTC 
CCCGGCCCAC CGGGCGCATC GATTACAACT GCTGATGGGA AAGCCGGCAA TGTTCAACAA 
GTGCAGGGAC CTCCCGGGCC GGAGGGTCCT CAGGGGATTC AAGGCAACGC CGGGTTACCT 
GGTTTGCCCG GAGAGAAAGG AAGTCAGGGT CTAAGAGGGG ACAGGGGGGA GTCCGGAGAG 
CGGGGAGAAC AGGGGCCGTC CGGAAACGAT GGGAGACCAG GAGTTGATGG AATGCCAGGA 
GCGGCCGGAA TCCCCGGAAG AGACGGGAAA ATGGGACCAA AGGGCGACTC AGGGGCGGAC 
GGAATCCCCG GGAAAGACGG AGTTCCGGGA AAACACGGTA AACATGGCGA ACGTGGAGAG 
AAAGGAGAGC CTGGAGAACC GGGACAACCT GGGAAACCCG GCCCCCCAGG ACCTGCATAC 
TCCCCGTTCA TGAGTGACGA TCCGAACGCA ATCCTGGATT ATGATTACAA ATACAGAGGC 
GAACAGGGAA TAAATGGACA GCCAGGACCC CCAGGAAAGG ACGGACGCTC CGGAGTTCCC 
GGTAGCCCCG GGATACCAGG GGAGCCCGGA CAGCCCGGGA GACCGGGAGA AATGGGGGAT 
CAAGGTGACC CAGGAGATGT GGGACCAAGA GGGTTACCGG GAGTTCCCGG ACTGAAAGGT 
AGCCATGGCG ACCCAGGCAT GGACGGCATG CAGGGAGATC AGGGAGTGGC AGGTGCCCCG 
GGACCTCGCG GGGAGCCAGG AAAAGACGGA AGACCGGGAC AGCCAGGGAC CCAAGGAATA 
TCAGGTCCTA TGGGACCTAT TGGTCCGATT GGACAACCCG GACCTCCTGG TCCCCCCGGA 
CCTCCAGGAC CTTCGTTTGA TGTGAAATCT ATAATTGGCG GTGGTATGTC AGATAGCAGC 
ATGTTTGGTC CAAGTGGTGC CCCGGTAAGT TGTCACCAGC GGCAAGGATA TCCGGGCCCC 
TCAGGTGCCC CCGGACTCAC AGGAATGCCA GGAGAGCGTG GGCCATCGGG AGAGCGGGGT 
ACACCTGGGT CCACGGGTGC CCCCGGAGTT CCTGGTATTT CCGGCGCCCC GGGCCAACAA 
GGACCACCGG GTCACAGAGG TCAACCTGGA ATGCCGGGAA AACCAGGACT ACCAGGACGC 
ATGATTCCAG AGTCTGAGAT CAGGGAGCTT TGTATGAGAC TACTGAGAGA AAACTTTGAT 
GAATTGGTCG CCGGACTTCG AGGACCGCCC GGGCCCCCGG GCGTTGGGAA ACCAGGAGCT 
CAGGGATCTC CAGGCCGGGT GGGCGCTCAA GGTGAGCGTG GGTTACCTGG ACCAACGGGT 
GAGCGTGGTT ATGCCGGCAT GCCAGGACTT CCAGGCCCCA TGGGACCTCA AGGTCCTGCG 
GGACCTGAGG GTGTGAAAGG AGACAGGGGA GAAATTGGGG AAGGAATTCA GGGCAAACAA 
GGGAACCCTG GAATCCCAGG TCCTCCTGGA CCTCCAGGCG AGGGGGTTAT GGGTCGCCCT 
GGAGAAAGGG GAAACCCCGG AGCCCATGGT CAACCAGGCA GTGCCGGTCC CCCAGGAAAA 
ACAGGACCCC CCGGATATTG TGAATACTGT AACTTTGCAT CCGGTAGAAG ATCTGGAGAT 
TATATACAAC TTGGTCCAGG CCGATCAAAC ACCAAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019922121.1
CDS	366..3407
Translation	MLPRLRSAHPGLLISAIFILYIPTSYCSKCDYTVYGKDDAPEYDFISSFNNFKRVIGVRIDRRSGTQVGQTSYEFRRRAERGDLIIPTRNLFSDGFPSEFSLSVTLKLASVTRRESWNLIDITNPQGETDFAVRFVGELRQIHLLYRDKVLNTLTTVEFDKKDTRNVFTKGWHKMQISVQKTEASLYIDCTRIQTVQMTPSDVSNIGYMTVAKSMTSKHTTPKFYLQWLAMHCDPTKPSRDSCEELEDIDDSDSQTYKDIMLADKQQRGGSKCPTQCPPGKNGIPGKQGVSGLPGKPGTPGQDGLPGERGPAGKDGNDGRPGEPGKPGLLGFQGQPGERGERGLNGKDGSAGEPGKPGKDGSVGPRGLQGEEGPRGERGETGSPGPAGPPGESVQGPEGVEGPQGPPGQPGPLGPQGVPGQQGNPGKDGKPGERGEKGDSGMPGLPGAQGAQGLQGVPGLPGPPGASITTADGKAGNVQQVQGPPGPEGPQGIQGNAGLPGLPGEKGSQGLRGDRGESGERGEQGPSGNDGRPGVDGMPGAAGIPGRDGKMGPKGDSGADGIPGKDGVPGKHGKHGERGEKGEPGEPGQPGKPGPPGPAYSPFMSDDPNAILDYDYKYRGEQGINGQPGPPGKDGRSGVPGSPGIPGEPGQPGRPGEMGDQGDPGDVGPRGLPGVPGLKGSHGDPGMDGMQGDQGVAGAPGPRGEPGKDGRPGQPGTQGISGPMGPIGPIGQPGPPGPPGPPGPSFDVKSIIGGGMSDSSMFGPSGAPVSCHQRQGYPGPSGAPGLTGMPGERGPSGERGTPGSTGAPGVPGISGAPGQQGPPGHRGQPGMPGKPGLPGRMIPESEIRELCMRLLRENFDELVAGLRGPPGPPGVGKPGAQGSPGRVGAQGERGLPGPTGERGYAGMPGLPGPMGPQGPAGPEGVKGDRGEIGEGIQGKQGNPGIPGPPGPPGEGVMGRPGERGNPGAHGQPGSAGPPGKTGPPGYCEYCNFASGRRSGDYIQLGPGRSNTKG

Target ORF information:

RefSeq Version	XM_020066562.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020066562.1

ORF Insert Sequence:

ATGCTGCCAA GATTACGATC CGCACACCCT GGGTTGCTTA TATCAGCTAT ATTTATTCTA 
TACATTCCAA CTTCTTACTG CTCAAAATGT GATTACACGG TATACGGAAA GGATGACGCA 
CCAGAGTATG ATTTCATATC CTCCTTCAAC AACTTTAAGC GAGTGATCGG GGTGAGGATT 
GACAGGAGGA GCGGTACCCA GGTGGGACAG ACATCCTACG AGTTCAGGAG GAGAGCGGAG 
CGGGGGGACT TGATCATTCC AACAAGAAAT TTGTTTTCGG ACGGGTTTCC GTCGGAGTTC 
TCTCTATCGG TTACCTTGAA GCTGGCCAGT GTAACGAGGC GCGAGTCATG GAACCTGATC 
GACATCACGA ACCCCCAGGG CGAGACAGAC TTCGCTGTGC GCTTTGTTGG GGAGTTACGA 
CAAATTCATC TCTTGTACAG GGACAAGGTT CTCAATACGC TAACAACGGT CGAATTCGAC 
AAAAAAGACA CGCGAAATGT GTTTACAAAG GGATGGCACA AGATGCAGAT TAGCGTTCAA 
AAGACTGAGG CCTCTCTATA TATTGACTGC ACAAGGATAC AGACCGTCCA GATGACGCCA 
TCTGATGTCA GCAACATAGG GTACATGACC GTCGCAAAAT CAATGACTTC CAAACACACC 
ACACCAAAGT TTTACCTGCA GTGGCTAGCT ATGCACTGTG ATCCCACCAA GCCCTCCAGG 
GACAGCTGCG AGGAGTTGGA GGATATTGAC GACAGCGATT CTCAAACATA CAAGGATATC 
ATGTTGGCAG ACAAGCAACA ACGTGGAGGA TCCAAATGTC CCACCCAATG TCCGCCCGGA 
AAAAACGGAA TCCCAGGAAA ACAAGGAGTT TCCGGTCTTC CCGGCAAACC AGGAACCCCC 
GGACAAGATG GACTTCCGGG AGAAAGAGGA CCGGCAGGAA AAGACGGAAA CGACGGACGA 
CCTGGAGAGC CCGGTAAACC CGGACTGCTG GGATTTCAGG GTCAGCCCGG AGAGAGGGGA 
GAACGAGGCT TGAATGGAAA GGACGGAAGC GCCGGAGAGC CTGGCAAGCC AGGAAAAGAT 
GGAAGTGTTG GACCAAGAGG ACTGCAGGGA GAAGAGGGAC CACGGGGAGA GAGGGGAGAA 
ACTGGTTCCC CCGGCCCTGC CGGACCCCCC GGAGAAAGCG TTCAAGGACC TGAAGGAGTT 
GAAGGTCCCC AGGGACCCCC AGGTCAACCA GGACCTCTGG GACCACAGGG AGTTCCCGGG 
CAACAAGGCA ATCCCGGCAA GGACGGTAAA CCAGGAGAGA GGGGGGAGAA GGGAGACAGC 
GGTATGCCTG GTCTACCGGG GGCACAAGGG GCCCAAGGTT TGCAGGGTGT TCCAGGACTC 
CCCGGCCCAC CGGGCGCATC GATTACAACT GCTGATGGGA AAGCCGGCAA TGTTCAACAA 
GTGCAGGGAC CTCCCGGGCC GGAGGGTCCT CAGGGGATTC AAGGCAACGC CGGGTTACCT 
GGTTTGCCCG GAGAGAAAGG AAGTCAGGGT CTAAGAGGGG ACAGGGGGGA GTCCGGAGAG 
CGGGGAGAAC AGGGGCCGTC CGGAAACGAT GGGAGACCAG GAGTTGATGG AATGCCAGGA 
GCGGCCGGAA TCCCCGGAAG AGACGGGAAA ATGGGACCAA AGGGCGACTC AGGGGCGGAC 
GGAATCCCCG GGAAAGACGG AGTTCCGGGA AAACACGGTA AACATGGCGA ACGTGGAGAG 
AAAGGAGAGC CTGGAGAACC GGGACAACCT GGGAAACCCG GCCCCCCAGG ACCTGCATAC 
TCCCCGTTCA TGAGTGACGA TCCGAACGCA ATCCTGGATT ATGATTACAA ATACAGAGGC 
GAACAGGGAA TAAATGGACA GCCAGGACCC CCAGGAAAGG ACGGACGCTC CGGAGTTCCC 
GGTAGCCCCG GGATACCAGG GGAGCCCGGA CAGCCCGGGA GACCGGGAGA AATGGGGGAT 
CAAGGTGACC CAGGAGATGT GGGACCAAGA GGGTTACCGG GAGTTCCCGG ACTGAAAGGT 
AGCCATGGCG ACCCAGGCAT GGACGGCATG CAGGGAGATC AGGGAGTGGC AGGTGCCCCG 
GGACCTCGCG GGGAGCCAGG AAAAGACGGA AGACCGGGAC AGCCAGGGAC CCAAGGAATA 
TCAGGTCCTA TGGGACCTAT TGGTCCGATT GGACAACCCG GACCTCCTGG TCCCCCCGGA 
CCTCCAGGAC CTTCGTTTGA TGTGAAATCT ATAATTGGCG GTGGTATGTC AGATAGCAGC 
ATGTTTGGTC CAAGTGGTGC CCCGGTAAGT TGTCACCAGC GGCAAGGATA TCCGGGCCCC 
TCAGGTGCCC CCGGACTCAC AGGAATGCCA GGAGAGCGTG GGCCATCGGG AGAGCGGGGT 
ACACCTGGGT CCACGGGTGC CCCCGGAGTT CCTGGTATTT CCGGCGCCCC GGGCCAACAA 
GGACCACCGG GTCACAGAGG TCAACCTGGA ATGCCGGGAA AACCAGGACT ACCAGGACGC 
ATGATTCCAG AGTCTGAGAT CAGGGAGCTT TGTATGAGAC TACTGAGAGA AAACTTTGAT 
GAATTGGTCG CCGGACTTCG AGGACCGCCC GGGCCCCCGG GCGTTGGGAA ACCAGGAGCT 
CAGGGATCTC CAGGCCGGGT GGGCGCTCAA GGTGAGCGTG GGTTACCTGG ACCAACGGGT 
GAGCGTGGTT ATGCCGGCAT GCCAGGACTT CCAGGCCCCA TGGGACCTCA AGGTCCTGCG 
GGACCTGAGG GTGTGAAAGG AGACAGGGGA GAAATTGGGG AAGGAATTCA GGGCAAACAA 
GGGAACCCTG GAATCCCAGG TCCTCCTGGA CCTCCAGGCG AGGGGGTTAT GGGTCGCCCT 
GGAGAAAGGG GAAACCCCGG AGCCCATGGT CAACCAGGCA GTGCCGGTCC CCCAGGAAAA 
ACAGGACCCC CCGGATATTG TGAATACTGT AACTTTGCAT CCGGTAGAAG ATCTGGAGAT 
TATATACAAC TTGGTCCAGG CCGATCAAAC ACCAAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105326709 ( XM_011426881.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011425183.1 Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr91265
Clone ID Related Accession (Same CDS sequence)	XM_011426881.2
Accession Version	XM_011426881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	collagen alpha-1(I) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011935082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011426881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCAA GATTACGATC CGCACACCCT GGGTTGCTTA TATCAGCTAT ATTTATTCTA 
TACATTCCAA CTTCTTACTG CTCAAAATGT GATTACACGG TATACGGAAA GGATGACGCA 
CCAGAGTATG ATTTCATATC CTCCTTCAAC AACTTTAAGC GAGTGATCGG GGTGAGGATT 
GACAGGAGGA GCGGTACCCA GGTGGGACAG ACATCCTACG AGTTCAGGAG GAGAGCGGAG 
CGGGGGGACT TGATCATTCC AACAAGAAAT TTGTTTTCGG ACGGGTTTCC GTCGGAGTTC 
TCTCTATCGG TTACCTTGAA GCTGGCCAGT GTAACGAGGC GCGAGTCATG GAACCTGATC 
GACATCACGA ACCCCCAGGG CGAGACAGAC TTCGCTGTGC GCTTTGTTGG GGAGTTACGA 
CAAATTCATC TCTTGTACAG GGACAAGGTT CTCAATACGC TAACAACGGT CGAATTCGAC 
AAAAAAGACA CGCGAAATGT GTTTACAAAG GGATGGCACA AGATGCAGAT TAGCGTTCAA 
AAGACTGAGG CCTCTCTATA TATTGACTGC ACAAGGATAC AGACCGTCCA GATGACGCCA 
TCTGATGTCA GCAACATAGG GTACATGACC GTCGCAAAAT CAATGACTTC CAAACACACC 
ACACCAAAGT TTTACCTGCA GTGGCTAGCT ATGCACTGTG ATCCCACCAA GCCCTCCAGG 
GACAGCTGCG AGGAGTTGGA GGATATTGAC GACAGCGATT CTCAAACATA CAAGGATATC 
ATGTTGGCAG ACAAGCAACA ACGTGGAGGA TCCAAATGTC CCACCCAATG TCCGCCCGGA 
AAAAACGGAA TCCCAGGAAA ACAAGGAGTT TCCGGTCTTC CCGGCAAACC AGGAACCCCC 
GGACAAGATG GACTTCCGGG AGAAAGAGGA CCGGCAGGAA AAGACGGAAA CGACGGACGA 
CCTGGAGAGC CCGGTAAACC CGGACTGCTG GGATTTCAGG GTCAGCCCGG AGAGAGGGGA 
GAACGAGGCT TGAATGGAAA GGACGGAAGC GCCGGAGAGC CTGGCAAGCC AGGAAAAGAT 
GGAAGTGTTG GACCAAGAGG ACTGCAGGGA GAAGAGGGAC CACGGGGAGA GAGGGGAGAA 
ACTGGTTCCC CCGGCCCTGC CGGACCCCCC GGAGAAAGCG TTCAAGGACC TGAAGGAGTT 
GAAGGTCCCC AGGGACCCCC AGGTCAACCA GGACCTCTGG GACCACAGGG AGTTCCCGGG 
CAACAAGGCA ATCCCGGCAA GGACGGTAAA CCAGGAGAGA GGGGGGAGAA GGGAGACAGC 
GGTATGCCTG GTCTACCGGG GGCACAAGGG GCCCAAGGTT TGCAGGGTGT TCCAGGACTC 
CCCGGCCCAC CGGGCGCATC GATTACAACT GCTGATGGGA AAGCCGGCAA TGTTCAACAA 
GTGCAGGGAC CTCCCGGGCC GGAGGGTCCT CAGGGGATTC AAGGCAACGC CGGGTTACCT 
GGTTTGCCCG GAGAGAAAGG AAGTCAGGGT CTAAGAGGGG ACAGGGGGGA GTCCGGAGAG 
CGGGGAGAAC AGGGGCCGTC CGGAAACGAT GGGAGACCAG GAGTTGATGG AATGCCAGGA 
GCGGCCGGAA TCCCCGGAAG AGACGGGAAA ATGGGACCAA AGGGCGACTC AGGGGCGGAC 
GGAATCCCCG GGAAAGACGG AGTTCCGGGA AAACACGGTA AACATGGCGA ACGTGGAGAG 
AAAGGAGAGC CTGGAGAACC GGGACAACCT GGGAAACCCG GCCCCCCAGG ACCTGCATAC 
TCCCCGTTCA TGAGTGACGA TCCGAACGCA ATCCTGGATT ATGATTACAA ATACAGAGGC 
GAACAGGGAA TAAATGGACA GCCAGGACCC CCAGGAAAGG ACGGACGCTC CGGAGTTCCC 
GGTAGCCCCG GGATACCAGG GGAGCCCGGA CAGCCCGGGA GACCGGGAGA AATGGGGGAT 
CAAGGTGACC CAGGAGATGT GGGACCAAGA GGGTTACCGG GAGTTCCCGG ACTGAAAGGT 
AGCCATGGCG ACCCAGGCAT GGACGGCATG CAGGGAGATC AGGGAGTGGC AGGTGCCCCG 
GGACCTCGCG GGGAGCCAGG AAAAGACGGA AGACCGGGAC AGCCAGGGAC CCAAGGAATA 
TCAGGTCCTA TGGGACCTAT TGGTCCGATT GGACAACCCG GACCTCCTGG TCCCCCCGGA 
CCTCCAGGAC CTTCGTTTGA TGTGAAATCT ATAATTGGCG GTGGTATGTC AGATAGCAGC 
ATGTTTGGTC CAAGTGGTGC CCCGGGCCCC TCAGGTGCCC CCGGACTCAC AGGAATGCCA 
GGAGAGCGTG GGCCATCGGG AGAGCGGGGT ACACCTGGGT CCACGGGTGC CCCCGGAGTT 
CCTGGTATTT CCGGCGCCCC GGGCCAACAA GGACCACCGG GTCACAGAGG TCAACCTGGA 
ATGCCGGGAA AACCAGGACT ACCAGGACGC ATGATTCCAG AGTCTGAGAT CAGGGAGCTT 
TGTATGAGAC TACTGAGAGA AAACTTTGAT GAATTGGTCG CCGGACTTCG AGGACCGCCC 
GGGCCCCCGG GCGTTGGGAA ACCAGGAGCT CAGGGATCTC CAGGCCGGGT GGGCGCTCAA 
GGTGAGCGTG GGTTACCTGG ACCAACGGGT GAGCGTGGTT ATGCCGGCAT GCCAGGACTT 
CCAGGCCCCA TGGGACCTCA AGGTCCTGCG GGACCTGAGG GTGTGAAAGG AGACAGGGGA 
GAAATTGGGG AAGGAATTCA GGGCAAACAA GGGAACCCTG GAATCCCAGG TCCTCCTGGA 
CCTCCAGGCG AGGGGGTTAT GGGTCGCCCT GGAGAAAGGG GAAACCCCGG AGCCCATGGT 
CAACCAGGCA GTGCCGGTCC CCCAGGAAAA ACAGGACCCC CCGGATATTG TGAATACTGT 
AACTTTGCAT CCGGTAGAAG ATCTGGAGAT TATATACAAC TTGGTCCAGG CCGATCAAAC 
ACCAAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011425183.1
CDS	362..3373
Translation	MLPRLRSAHPGLLISAIFILYIPTSYCSKCDYTVYGKDDAPEYDFISSFNNFKRVIGVRIDRRSGTQVGQTSYEFRRRAERGDLIIPTRNLFSDGFPSEFSLSVTLKLASVTRRESWNLIDITNPQGETDFAVRFVGELRQIHLLYRDKVLNTLTTVEFDKKDTRNVFTKGWHKMQISVQKTEASLYIDCTRIQTVQMTPSDVSNIGYMTVAKSMTSKHTTPKFYLQWLAMHCDPTKPSRDSCEELEDIDDSDSQTYKDIMLADKQQRGGSKCPTQCPPGKNGIPGKQGVSGLPGKPGTPGQDGLPGERGPAGKDGNDGRPGEPGKPGLLGFQGQPGERGERGLNGKDGSAGEPGKPGKDGSVGPRGLQGEEGPRGERGETGSPGPAGPPGESVQGPEGVEGPQGPPGQPGPLGPQGVPGQQGNPGKDGKPGERGEKGDSGMPGLPGAQGAQGLQGVPGLPGPPGASITTADGKAGNVQQVQGPPGPEGPQGIQGNAGLPGLPGEKGSQGLRGDRGESGERGEQGPSGNDGRPGVDGMPGAAGIPGRDGKMGPKGDSGADGIPGKDGVPGKHGKHGERGEKGEPGEPGQPGKPGPPGPAYSPFMSDDPNAILDYDYKYRGEQGINGQPGPPGKDGRSGVPGSPGIPGEPGQPGRPGEMGDQGDPGDVGPRGLPGVPGLKGSHGDPGMDGMQGDQGVAGAPGPRGEPGKDGRPGQPGTQGISGPMGPIGPIGQPGPPGPPGPPGPSFDVKSIIGGGMSDSSMFGPSGAPGPSGAPGLTGMPGERGPSGERGTPGSTGAPGVPGISGAPGQQGPPGHRGQPGMPGKPGLPGRMIPESEIRELCMRLLRENFDELVAGLRGPPGPPGVGKPGAQGSPGRVGAQGERGLPGPTGERGYAGMPGLPGPMGPQGPAGPEGVKGDRGEIGEGIQGKQGNPGIPGPPGPPGEGVMGRPGERGNPGAHGQPGSAGPPGKTGPPGYCEYCNFASGRRSGDYIQLGPGRSNTKG

Target ORF information:

RefSeq Version	XM_011426881.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas collagen alpha-1(I) chain (LOC105326709), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011426881.2

ORF Insert Sequence:

ATGCTGCCAA GATTACGATC CGCACACCCT GGGTTGCTTA TATCAGCTAT ATTTATTCTA 
TACATTCCAA CTTCTTACTG CTCAAAATGT GATTACACGG TATACGGAAA GGATGACGCA 
CCAGAGTATG ATTTCATATC CTCCTTCAAC AACTTTAAGC GAGTGATCGG GGTGAGGATT 
GACAGGAGGA GCGGTACCCA GGTGGGACAG ACATCCTACG AGTTCAGGAG GAGAGCGGAG 
CGGGGGGACT TGATCATTCC AACAAGAAAT TTGTTTTCGG ACGGGTTTCC GTCGGAGTTC 
TCTCTATCGG TTACCTTGAA GCTGGCCAGT GTAACGAGGC GCGAGTCATG GAACCTGATC 
GACATCACGA ACCCCCAGGG CGAGACAGAC TTCGCTGTGC GCTTTGTTGG GGAGTTACGA 
CAAATTCATC TCTTGTACAG GGACAAGGTT CTCAATACGC TAACAACGGT CGAATTCGAC 
AAAAAAGACA CGCGAAATGT GTTTACAAAG GGATGGCACA AGATGCAGAT TAGCGTTCAA 
AAGACTGAGG CCTCTCTATA TATTGACTGC ACAAGGATAC AGACCGTCCA GATGACGCCA 
TCTGATGTCA GCAACATAGG GTACATGACC GTCGCAAAAT CAATGACTTC CAAACACACC 
ACACCAAAGT TTTACCTGCA GTGGCTAGCT ATGCACTGTG ATCCCACCAA GCCCTCCAGG 
GACAGCTGCG AGGAGTTGGA GGATATTGAC GACAGCGATT CTCAAACATA CAAGGATATC 
ATGTTGGCAG ACAAGCAACA ACGTGGAGGA TCCAAATGTC CCACCCAATG TCCGCCCGGA 
AAAAACGGAA TCCCAGGAAA ACAAGGAGTT TCCGGTCTTC CCGGCAAACC AGGAACCCCC 
GGACAAGATG GACTTCCGGG AGAAAGAGGA CCGGCAGGAA AAGACGGAAA CGACGGACGA 
CCTGGAGAGC CCGGTAAACC CGGACTGCTG GGATTTCAGG GTCAGCCCGG AGAGAGGGGA 
GAACGAGGCT TGAATGGAAA GGACGGAAGC GCCGGAGAGC CTGGCAAGCC AGGAAAAGAT 
GGAAGTGTTG GACCAAGAGG ACTGCAGGGA GAAGAGGGAC CACGGGGAGA GAGGGGAGAA 
ACTGGTTCCC CCGGCCCTGC CGGACCCCCC GGAGAAAGCG TTCAAGGACC TGAAGGAGTT 
GAAGGTCCCC AGGGACCCCC AGGTCAACCA GGACCTCTGG GACCACAGGG AGTTCCCGGG 
CAACAAGGCA ATCCCGGCAA GGACGGTAAA CCAGGAGAGA GGGGGGAGAA GGGAGACAGC 
GGTATGCCTG GTCTACCGGG GGCACAAGGG GCCCAAGGTT TGCAGGGTGT TCCAGGACTC 
CCCGGCCCAC CGGGCGCATC GATTACAACT GCTGATGGGA AAGCCGGCAA TGTTCAACAA 
GTGCAGGGAC CTCCCGGGCC GGAGGGTCCT CAGGGGATTC AAGGCAACGC CGGGTTACCT 
GGTTTGCCCG GAGAGAAAGG AAGTCAGGGT CTAAGAGGGG ACAGGGGGGA GTCCGGAGAG 
CGGGGAGAAC AGGGGCCGTC CGGAAACGAT GGGAGACCAG GAGTTGATGG AATGCCAGGA 
GCGGCCGGAA TCCCCGGAAG AGACGGGAAA ATGGGACCAA AGGGCGACTC AGGGGCGGAC 
GGAATCCCCG GGAAAGACGG AGTTCCGGGA AAACACGGTA AACATGGCGA ACGTGGAGAG 
AAAGGAGAGC CTGGAGAACC GGGACAACCT GGGAAACCCG GCCCCCCAGG ACCTGCATAC 
TCCCCGTTCA TGAGTGACGA TCCGAACGCA ATCCTGGATT ATGATTACAA ATACAGAGGC 
GAACAGGGAA TAAATGGACA GCCAGGACCC CCAGGAAAGG ACGGACGCTC CGGAGTTCCC 
GGTAGCCCCG GGATACCAGG GGAGCCCGGA CAGCCCGGGA GACCGGGAGA AATGGGGGAT 
CAAGGTGACC CAGGAGATGT GGGACCAAGA GGGTTACCGG GAGTTCCCGG ACTGAAAGGT 
AGCCATGGCG ACCCAGGCAT GGACGGCATG CAGGGAGATC AGGGAGTGGC AGGTGCCCCG 
GGACCTCGCG GGGAGCCAGG AAAAGACGGA AGACCGGGAC AGCCAGGGAC CCAAGGAATA 
TCAGGTCCTA TGGGACCTAT TGGTCCGATT GGACAACCCG GACCTCCTGG TCCCCCCGGA 
CCTCCAGGAC CTTCGTTTGA TGTGAAATCT ATAATTGGCG GTGGTATGTC AGATAGCAGC 
ATGTTTGGTC CAAGTGGTGC CCCGGGCCCC TCAGGTGCCC CCGGACTCAC AGGAATGCCA 
GGAGAGCGTG GGCCATCGGG AGAGCGGGGT ACACCTGGGT CCACGGGTGC CCCCGGAGTT 
CCTGGTATTT CCGGCGCCCC GGGCCAACAA GGACCACCGG GTCACAGAGG TCAACCTGGA 
ATGCCGGGAA AACCAGGACT ACCAGGACGC ATGATTCCAG AGTCTGAGAT CAGGGAGCTT 
TGTATGAGAC TACTGAGAGA AAACTTTGAT GAATTGGTCG CCGGACTTCG AGGACCGCCC 
GGGCCCCCGG GCGTTGGGAA ACCAGGAGCT CAGGGATCTC CAGGCCGGGT GGGCGCTCAA 
GGTGAGCGTG GGTTACCTGG ACCAACGGGT GAGCGTGGTT ATGCCGGCAT GCCAGGACTT 
CCAGGCCCCA TGGGACCTCA AGGTCCTGCG GGACCTGAGG GTGTGAAAGG AGACAGGGGA 
GAAATTGGGG AAGGAATTCA GGGCAAACAA GGGAACCCTG GAATCCCAGG TCCTCCTGGA 
CCTCCAGGCG AGGGGGTTAT GGGTCGCCCT GGAGAAAGGG GAAACCCCGG AGCCCATGGT 
CAACCAGGCA GTGCCGGTCC CCCAGGAAAA ACAGGACCCC CCGGATATTG TGAATACTGT 
AACTTTGCAT CCGGTAGAAG ATCTGGAGAT TATATACAAC TTGGTCCAGG CCGATCAAAC 
ACCAAAGGCT AA