BUB1 cDNA ORF clone, Corvus cornix cornix

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	104691552
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Corvus cornix cornix

mRNA and Protein(s)

mRNA	Protein	Name
XM_019287964.2	XP_019143509.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_019287965.2	XP_019143510.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2

Pathways from BioSystems
Gene Ontology

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo106607	XM_019287964.2 Latest version!	Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCo106608	XM_019287965.2 Latest version!	Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( XM_019287964.2 ) cDNA ORF clone, Corvus cornix cornix -> XP_019143509.2 Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo106607
Clone ID Related Accession (Same CDS sequence)	XM_019287964.2
Accession Version	XM_019287964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-21
Organism	Corvus cornix cornix
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114032.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 22, 2017 this sequence version replaced XM_019287964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus cornix cornix Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGCA GGTACCTGCA GTGGGCAGAG GAATGTCTCC CGCTCCAAGA GAAGCAGAGC 
CGCTGGCCCG GGCTCCTGGA GGAGCTGGTG AAGGTGTTCG TGGGCGACAA GAGGTACCAC 
CAGGACCTGC GGTTTGTGAG CTACTGCGTC AAGCTGGCAG AGTTCATCTC TTCCCCTTGC 
CAGTACTTTG AGTACTTGCA CGGGCAGGGA ATCGGAGCCA AGACCTCAGA TTTCTATCTG 
GCCTGGGCTC AGCTGCTCCT GAGGGAAGGC AACGTGCAGG GAGCAGGAGC TGTGCTCCAG 
AAAGGGCTCC TCAACCAGGC ACAGCCCCGG GAGCACCTGC AGCAGCTCCA CTGCTCCCTG 
CAGAGTTATG ATCCTCGGAA TCCTCCTTTG CAAGATGCTG CTGTTGTAAA ACCACTTCAG 
ACTTCCCATG CTGCAAATCA AATGGCTCCC CCAAAAGGTG TTTCAAGTCC TAATCCCTGC 
AAAAATCAGG GTCTGGATGC TGCTGGTTCC CAGTCCTGTG CTGTTGGAAA AGAAGTGAAC 
TACGTGACCT ACATCTCCAA GTCCGAGGTT GTGCCGAAGG CGCCGTCGGG TGCCACGGGG 
TGGGAGCAGG TCCCGATGTA CGACAAGAAC CTGCTCCTGT GCGAAGGCTC TGAGCTCTCC 
TTCGAGGAAC TGAGGGCCAA GAGATACTTC AGGAAATATG AGCTCCTCAG GAGGCAGCAG 
GAACTGGAGG AGGAGAAGAA AGACTCCATG AAGAAAAAGG AGTCGGCAGT CCTGGAGCTC 
CAAGCCCTGC AGCAGAAGCT GGACCAGCTG ACCCAGCTCT CCAGAAGCCT AGAGGAAACG 
AGACTGGAAC CAGCAGCTGA GCCAAACCAG AGGCTGGTGC CCCCCCCTGT GAGGCCCAGC 
ACATCCCACG TGCCAGGGAA CTGGATGGCA CCTGCTCCAG GGCTGGATGC GCCAAAGTCC 
CAAGCTCTGG TGCTGGAGCA GTCCCAGTTC CAGGTGCCAC CAGCCACCAC TCCCACCCTG 
CAGCCAGCAA AGCCTCTCGG TGACTGCACT CCAGCTGCCT CCCCCTGGGA AGCACCAGAG 
GAGAAGGACC CAGCTGGAGC TCAGGCAGAG CTTGGAGAGC TCCAGAAGAG TTTGCTGCAC 
CCTCCCTTCA GCGCTGCTCC AGCTCAGGAA CGGGCTCATC CCGACCCAGC TCTGCCTCGG 
AGCACGGGAG AACTGTCCCC TAACAAGGGA TCCACTGGAT TAACTCCTGC TGTGCATGTG 
AAGGAAGCCT CCAGAGCTGG GAATTCCTCC TTTGCCTCTG GAAATATTTC CCAAGCCACC 
CCCAACACCT CACTGGGAGG AGCAATGGGC ACAACAACGC CCTTCAAGGT GCAGCCGTCG 
CCCACGGTCC ACACGAAAGA AGCCTTGGGT GTTCTCATGG ATATGTTCCA AACACCATTC 
TTGCCTGAGC CATCTGCCCT GGAAGACAGC GAGGAACAAT TTGAAGCCTT TTGCAGAAGA 
AGAGAGCCTG GTGGATGTTT GAAAACCAAC ATCGTTGTCC CTGTCACTCC TGCATTTGCC 
ATCTTTGAGG ATGAGGAGGA GAAGGAGAAC AGCAGGATCC CACAGCACAA GAACAAACCA 
GAAGAGCCCA GAACTTTTGG AGAACGTCCC TTGACCGACT GTGCAGCAGA AGAAGAAACG 
GGAACCACGG AATTCCTGCG GGATGATTAC ACGGTGTGGA ATGGCCCCTG CAGAAACAAA 
GCCCTGGCTC CCAGTCCAGA CAACACCAGG GACTTTGCCC GAGCTGCCCA GCTCATGTCC 
ACACCCTTTA ATTACCTGGC AGCTCCTTCG AGGCCCCCTT TGGAGGACAG AGCCTGTGAG 
GAAAATTTGC CTCAAATGGA TTTGGAGTTC TCTGGAGAAC TACACAGGCA GCCAAAGGCC 
AAAAAGTTAA GGCCTGTTCT TGAACACATC CCTGAACAGA GAGAGCTTCA TGGAAACCTT 
CTGAAACAAG GGACTGGAAT CCAGGGAATT GCTGCAGCTG CTTCCCAACG ACTGCATGAG 
GAGACAGCTC CAAGCAGAAC TGGATATTCC TCCTCCCTTG GAGTGGATAG AACTTGCCAG 
GAGAGGCTGT GTGGAGCTGG GCAGGACAGG ACAGCCCGGA CTGGGAGACC TGCAGTCCTT 
GTTGAGAACC CTTGGGACAC GGAATTGGTT GGCAAGTTCT TGTCGGAGCT TCCAAAACCG 
CTCCACACCT ACGCCAACTA CTTTGAGTGG AAATCTCCTC TTCCATTCAT CAAACTGAAG 
GCTGAACTCT CACTGGGCTC CAGCTCATTC CACATGGATT GCTTGGTCGG AGAGGGAGCT 
TTTGCCCAAG TCTATCAAGC TTCCATCCTG GATGCCAGCA ACCCTCGGAA CAATCAGAAA 
GTGATATTCA AGGTCCAGAA ACCTGCCAAC CCTTGGGAGT TCTACATTGC CAGCCAGCTG 
CTGGAGAGGC TGAGCCCCAG CGTGCGCCAC CTCTACATCC ACTTCTATTC CGCCCATTTC 
TTCTCCAACG GCAGCATTTT GGTGGGGGAG CTCCACAACT ACGGAACGCT GCTCAATGCC 
GTCAACATTT ACAAGAAGCT CCCGGAGAAG GTGATGCCCC AAGCCCTCGT CATCTACTTT 
GCTGTCAAAA TCCTTCACAT GGTGGAGGAG CTGCACAGCT GCAAAATCAT CCATGGGGAC 
ATCAAACCTG ACAACTTCAT CCTGGGAGAA AGGTTCCTGG ACAACGACAC GTGTGACATT 
GATGGGCTCT GCCATGGCCT GACCCTCATT GACCTGGGCC AGAGCATCGA CATGAAGCTC 
TTCCCTGAGG GAACAGCCTT CACTGCCAGG TGTGAAACGT CTGGATTCCA GTGCGTGGAA 
ATGCTGACAA ACAAACCATG GAACTACCAG ACAGACTACT TTGGGATCGC AGCCACCGTG 
TACTGCCTGC TCTTCGGGAC CTACATGCGC CTCAAGGATG ACAATGGGGT CTGGAAGCCT 
GAGGGATCCT TCAGGAGGCT TGCCAATGCT GACCTGTGGA AAGAGTTCTT TGAGAGCTTG 
CTGAACATTC CCAGCTGCCA CAACCTGCCT TCCCTCGTAG TCCTGCGCCA GAAGCTCAAG 
GATTTATTTT GCAGGTCCTA CGCGAAGGAA ATCAAGTTCC TTCGGAACAG ACTCGTCGTG 
CTGCTCATCG AGCACAAACG CTCACGGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019143509.2
CDS	82..3294
Translation	MVCRYLQWAEECLPLQEKQSRWPGLLEELVKVFVGDKRYHQDLRFVSYCVKLAEFISSPCQYFEYLHGQGIGAKTSDFYLAWAQLLLREGNVQGAGAVLQKGLLNQAQPREHLQQLHCSLQSYDPRNPPLQDAAVVKPLQTSHAANQMAPPKGVSSPNPCKNQGLDAAGSQSCAVGKEVNYVTYISKSEVVPKAPSGATGWEQVPMYDKNLLLCEGSELSFEELRAKRYFRKYELLRRQQELEEEKKDSMKKKESAVLELQALQQKLDQLTQLSRSLEETRLEPAAEPNQRLVPPPVRPSTSHVPGNWMAPAPGLDAPKSQALVLEQSQFQVPPATTPTLQPAKPLGDCTPAASPWEAPEEKDPAGAQAELGELQKSLLHPPFSAAPAQERAHPDPALPRSTGELSPNKGSTGLTPAVHVKEASRAGNSSFASGNISQATPNTSLGGAMGTTTPFKVQPSPTVHTKEALGVLMDMFQTPFLPEPSALEDSEEQFEAFCRRREPGGCLKTNIVVPVTPAFAIFEDEEEKENSRIPQHKNKPEEPRTFGERPLTDCAAEEETGTTEFLRDDYTVWNGPCRNKALAPSPDNTRDFARAAQLMSTPFNYLAAPSRPPLEDRACEENLPQMDLEFSGELHRQPKAKKLRPVLEHIPEQRELHGNLLKQGTGIQGIAAAASQRLHEETAPSRTGYSSSLGVDRTCQERLCGAGQDRTARTGRPAVLVENPWDTELVGKFLSELPKPLHTYANYFEWKSPLPFIKLKAELSLGSSSFHMDCLVGEGAFAQVYQASILDASNPRNNQKVIFKVQKPANPWEFYIASQLLERLSPSVRHLYIHFYSAHFFSNGSILVGELHNYGTLLNAVNIYKKLPEKVMPQALVIYFAVKILHMVEELHSCKIIHGDIKPDNFILGERFLDNDTCDIDGLCHGLTLIDLGQSIDMKLFPEGTAFTARCETSGFQCVEMLTNKPWNYQTDYFGIAATVYCLLFGTYMRLKDDNGVWKPEGSFRRLANADLWKEFFESLLNIPSCHNLPSLVVLRQKLKDLFCRSYAKEIKFLRNRLVVLLIEHKRSRK

Target ORF information:

RefSeq Version	XM_019287964.2
Organism	Corvus cornix cornix
Definition	Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019287964.2

ORF Insert Sequence:

ATGGTTTGCA GGTACCTGCA GTGGGCAGAG GAATGTCTCC CGCTCCAAGA GAAGCAGAGC 
CGCTGGCCCG GGCTCCTGGA GGAGCTGGTG AAGGTGTTCG TGGGCGACAA GAGGTACCAC 
CAGGACCTGC GGTTTGTGAG CTACTGCGTC AAGCTGGCAG AGTTCATCTC TTCCCCTTGC 
CAGTACTTTG AGTACTTGCA CGGGCAGGGA ATCGGAGCCA AGACCTCAGA TTTCTATCTG 
GCCTGGGCTC AGCTGCTCCT GAGGGAAGGC AACGTGCAGG GAGCAGGAGC TGTGCTCCAG 
AAAGGGCTCC TCAACCAGGC ACAGCCCCGG GAGCACCTGC AGCAGCTCCA CTGCTCCCTG 
CAGAGTTATG ATCCTCGGAA TCCTCCTTTG CAAGATGCTG CTGTTGTAAA ACCACTTCAG 
ACTTCCCATG CTGCAAATCA AATGGCTCCC CCAAAAGGTG TTTCAAGTCC TAATCCCTGC 
AAAAATCAGG GTCTGGATGC TGCTGGTTCC CAGTCCTGTG CTGTTGGAAA AGAAGTGAAC 
TACGTGACCT ACATCTCCAA GTCCGAGGTT GTGCCGAAGG CGCCGTCGGG TGCCACGGGG 
TGGGAGCAGG TCCCGATGTA CGACAAGAAC CTGCTCCTGT GCGAAGGCTC TGAGCTCTCC 
TTCGAGGAAC TGAGGGCCAA GAGATACTTC AGGAAATATG AGCTCCTCAG GAGGCAGCAG 
GAACTGGAGG AGGAGAAGAA AGACTCCATG AAGAAAAAGG AGTCGGCAGT CCTGGAGCTC 
CAAGCCCTGC AGCAGAAGCT GGACCAGCTG ACCCAGCTCT CCAGAAGCCT AGAGGAAACG 
AGACTGGAAC CAGCAGCTGA GCCAAACCAG AGGCTGGTGC CCCCCCCTGT GAGGCCCAGC 
ACATCCCACG TGCCAGGGAA CTGGATGGCA CCTGCTCCAG GGCTGGATGC GCCAAAGTCC 
CAAGCTCTGG TGCTGGAGCA GTCCCAGTTC CAGGTGCCAC CAGCCACCAC TCCCACCCTG 
CAGCCAGCAA AGCCTCTCGG TGACTGCACT CCAGCTGCCT CCCCCTGGGA AGCACCAGAG 
GAGAAGGACC CAGCTGGAGC TCAGGCAGAG CTTGGAGAGC TCCAGAAGAG TTTGCTGCAC 
CCTCCCTTCA GCGCTGCTCC AGCTCAGGAA CGGGCTCATC CCGACCCAGC TCTGCCTCGG 
AGCACGGGAG AACTGTCCCC TAACAAGGGA TCCACTGGAT TAACTCCTGC TGTGCATGTG 
AAGGAAGCCT CCAGAGCTGG GAATTCCTCC TTTGCCTCTG GAAATATTTC CCAAGCCACC 
CCCAACACCT CACTGGGAGG AGCAATGGGC ACAACAACGC CCTTCAAGGT GCAGCCGTCG 
CCCACGGTCC ACACGAAAGA AGCCTTGGGT GTTCTCATGG ATATGTTCCA AACACCATTC 
TTGCCTGAGC CATCTGCCCT GGAAGACAGC GAGGAACAAT TTGAAGCCTT TTGCAGAAGA 
AGAGAGCCTG GTGGATGTTT GAAAACCAAC ATCGTTGTCC CTGTCACTCC TGCATTTGCC 
ATCTTTGAGG ATGAGGAGGA GAAGGAGAAC AGCAGGATCC CACAGCACAA GAACAAACCA 
GAAGAGCCCA GAACTTTTGG AGAACGTCCC TTGACCGACT GTGCAGCAGA AGAAGAAACG 
GGAACCACGG AATTCCTGCG GGATGATTAC ACGGTGTGGA ATGGCCCCTG CAGAAACAAA 
GCCCTGGCTC CCAGTCCAGA CAACACCAGG GACTTTGCCC GAGCTGCCCA GCTCATGTCC 
ACACCCTTTA ATTACCTGGC AGCTCCTTCG AGGCCCCCTT TGGAGGACAG AGCCTGTGAG 
GAAAATTTGC CTCAAATGGA TTTGGAGTTC TCTGGAGAAC TACACAGGCA GCCAAAGGCC 
AAAAAGTTAA GGCCTGTTCT TGAACACATC CCTGAACAGA GAGAGCTTCA TGGAAACCTT 
CTGAAACAAG GGACTGGAAT CCAGGGAATT GCTGCAGCTG CTTCCCAACG ACTGCATGAG 
GAGACAGCTC CAAGCAGAAC TGGATATTCC TCCTCCCTTG GAGTGGATAG AACTTGCCAG 
GAGAGGCTGT GTGGAGCTGG GCAGGACAGG ACAGCCCGGA CTGGGAGACC TGCAGTCCTT 
GTTGAGAACC CTTGGGACAC GGAATTGGTT GGCAAGTTCT TGTCGGAGCT TCCAAAACCG 
CTCCACACCT ACGCCAACTA CTTTGAGTGG AAATCTCCTC TTCCATTCAT CAAACTGAAG 
GCTGAACTCT CACTGGGCTC CAGCTCATTC CACATGGATT GCTTGGTCGG AGAGGGAGCT 
TTTGCCCAAG TCTATCAAGC TTCCATCCTG GATGCCAGCA ACCCTCGGAA CAATCAGAAA 
GTGATATTCA AGGTCCAGAA ACCTGCCAAC CCTTGGGAGT TCTACATTGC CAGCCAGCTG 
CTGGAGAGGC TGAGCCCCAG CGTGCGCCAC CTCTACATCC ACTTCTATTC CGCCCATTTC 
TTCTCCAACG GCAGCATTTT GGTGGGGGAG CTCCACAACT ACGGAACGCT GCTCAATGCC 
GTCAACATTT ACAAGAAGCT CCCGGAGAAG GTGATGCCCC AAGCCCTCGT CATCTACTTT 
GCTGTCAAAA TCCTTCACAT GGTGGAGGAG CTGCACAGCT GCAAAATCAT CCATGGGGAC 
ATCAAACCTG ACAACTTCAT CCTGGGAGAA AGGTTCCTGG ACAACGACAC GTGTGACATT 
GATGGGCTCT GCCATGGCCT GACCCTCATT GACCTGGGCC AGAGCATCGA CATGAAGCTC 
TTCCCTGAGG GAACAGCCTT CACTGCCAGG TGTGAAACGT CTGGATTCCA GTGCGTGGAA 
ATGCTGACAA ACAAACCATG GAACTACCAG ACAGACTACT TTGGGATCGC AGCCACCGTG 
TACTGCCTGC TCTTCGGGAC CTACATGCGC CTCAAGGATG ACAATGGGGT CTGGAAGCCT 
GAGGGATCCT TCAGGAGGCT TGCCAATGCT GACCTGTGGA AAGAGTTCTT TGAGAGCTTG 
CTGAACATTC CCAGCTGCCA CAACCTGCCT TCCCTCGTAG TCCTGCGCCA GAAGCTCAAG 
GATTTATTTT GCAGGTCCTA CGCGAAGGAA ATCAAGTTCC TTCGGAACAG ACTCGTCGTG 
CTGCTCATCG AGCACAAACG CTCACGGAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_019287965.2 ) cDNA ORF clone, Corvus cornix cornix -> XP_019143510.2 Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo106608
Clone ID Related Accession (Same CDS sequence)	XM_019287965.2
Accession Version	XM_019287965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-21
Organism	Corvus cornix cornix
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114032.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 22, 2017 this sequence version replaced XM_019287965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus cornix cornix Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTGCA GGTACCTGCA GTGGGCAGAG GAATGTCTCC CGCTCCAAGA GAAGCAGAGC 
CGCTGGCCCG GGCTCCTGGA GGAGCTGGTG AAGGTGTTCG TGGGCGACAA GAGGTACCAC 
CAGGACCTGC GGTTTGTGAG CTACTGCGTC AAGCTGGCAG AGTTCATCTC TTCCCCTTGC 
CAGTACTTTG AGTACTTGCA CGGGCAGGGA ATCGGAGCCA AGACCTCAGA TTTCTATCTG 
GCCTGGGCTC AGCTGCTCCT GAGGGAAGGC AACGTGCAGG GAGCAGGAGC TGTGCTCCAG 
AAAGGGCTCC TCAACCAGGC ACAGCCCCGG GAGCACCTGC AGCAGCTCCA CTGCTCCCTG 
CAGAGTTATG ATCCTCGGAA TCCTCCTTTG CAAGATGCTG CTGTTGTAAA ACCACTTCAG 
ACTTCCCATG CTGCAAATCA AATGGCTCCC CCAAAAGGTG TTTCAAGTCC TAATCCCTGC 
AAAAATCAGG GTCTGGATGC TGCTGGTTCC CAGTCCTGTG CTGTTGGAAA AGAAGTGAAC 
TACGTGACCT ACATCTCCAA GTCCGAGGTT GTGCCGAAGG CGCCGTCGGG TGCCACGGGG 
TGGGAGCAGG TCCCGATGTA CGACAAGAAC CTGCTCCTGT GCGAAGGCTC TGAGCTCTCC 
TTCGAGGAAC TGAGGGCCAA GAGATACTTC AGGAAATATG AGCTCCTCAG GAGGCAGCAG 
GAACTGGAGG AGGAGAAGAA AGACTCCATG AAGAAAAAGG AGTCGGCAGT CCTGGAGCTC 
CAAGCCCTGC AGCAGAAGCT GGACCAGCTG ACCCAGCTCT CCAGAAGCCT AGAGGAAACG 
AGACTGGAAC CAGCAGCTGA GCCAAACCAG AGGCTGCCAG CAAAGCCTCT CGGTGACTGC 
ACTCCAGCTG CCTCCCCCTG GGAAGCACCA GAGGAGAAGG ACCCAGCTGG AGCTCAGGCA 
GAGCTTGGAG AGCTCCAGAA GAGTTTGCTG CACCCTCCCT TCAGCGCTGC TCCAGCTCAG 
GAACGGGCTC ATCCCGACCC AGCTCTGCCT CGGAGCACGG GAGAACTGTC CCCTAACAAG 
GGATCCACTG GATTAACTCC TGCTGTGCAT GTGAAGGAAG CCTCCAGAGC TGGGAATTCC 
TCCTTTGCCT CTGGAAATAT TTCCCAAGCC ACCCCCAACA CCTCACTGGG AGGAGCAATG 
GGCACAACAA CGCCCTTCAA GGTGCAGCCG TCGCCCACGG TCCACACGAA AGAAGCCTTG 
GGTGTTCTCA TGGATATGTT CCAAACACCA TTCTTGCCTG AGCCATCTGC CCTGGAAGAC 
AGCGAGGAAC AATTTGAAGC CTTTTGCAGA AGAAGAGAGC CTGGTGGATG TTTGAAAACC 
AACATCGTTG TCCCTGTCAC TCCTGCATTT GCCATCTTTG AGGATGAGGA GGAGAAGGAG 
AACAGCAGGA TCCCACAGCA CAAGAACAAA CCAGAAGAGC CCAGAACTTT TGGAGAACGT 
CCCTTGACCG ACTGTGCAGC AGAAGAAGAA ACGGGAACCA CGGAATTCCT GCGGGATGAT 
TACACGGTGT GGAATGGCCC CTGCAGAAAC AAAGCCCTGG CTCCCAGTCC AGACAACACC 
AGGGACTTTG CCCGAGCTGC CCAGCTCATG TCCACACCCT TTAATTACCT GGCAGCTCCT 
TCGAGGCCCC CTTTGGAGGA CAGAGCCTGT GAGGAAAATT TGCCTCAAAT GGATTTGGAG 
TTCTCTGGAG AACTACACAG GCAGCCAAAG GCCAAAAAGT TAAGGCCTGT TCTTGAACAC 
ATCCCTGAAC AGAGAGAGCT TCATGGAAAC CTTCTGAAAC AAGGGACTGG AATCCAGGGA 
ATTGCTGCAG CTGCTTCCCA ACGACTGCAT GAGGAGACAG CTCCAAGCAG AACTGGATAT 
TCCTCCTCCC TTGGAGTGGA TAGAACTTGC CAGGAGAGGC TGTGTGGAGC TGGGCAGGAC 
AGGACAGCCC GGACTGGGAG ACCTGCAGTC CTTGTTGAGA ACCCTTGGGA CACGGAATTG 
GTTGGCAAGT TCTTGTCGGA GCTTCCAAAA CCGCTCCACA CCTACGCCAA CTACTTTGAG 
TGGAAATCTC CTCTTCCATT CATCAAACTG AAGGCTGAAC TCTCACTGGG CTCCAGCTCA 
TTCCACATGG ATTGCTTGGT CGGAGAGGGA GCTTTTGCCC AAGTCTATCA AGCTTCCATC 
CTGGATGCCA GCAACCCTCG GAACAATCAG AAAGTGATAT TCAAGGTCCA GAAACCTGCC 
AACCCTTGGG AGTTCTACAT TGCCAGCCAG CTGCTGGAGA GGCTGAGCCC CAGCGTGCGC 
CACCTCTACA TCCACTTCTA TTCCGCCCAT TTCTTCTCCA ACGGCAGCAT TTTGGTGGGG 
GAGCTCCACA ACTACGGAAC GCTGCTCAAT GCCGTCAACA TTTACAAGAA GCTCCCGGAG 
AAGGTGATGC CCCAAGCCCT CGTCATCTAC TTTGCTGTCA AAATCCTTCA CATGGTGGAG 
GAGCTGCACA GCTGCAAAAT CATCCATGGG GACATCAAAC CTGACAACTT CATCCTGGGA 
GAAAGGTTCC TGGACAACGA CACGTGTGAC ATTGATGGGC TCTGCCATGG CCTGACCCTC 
ATTGACCTGG GCCAGAGCAT CGACATGAAG CTCTTCCCTG AGGGAACAGC CTTCACTGCC 
AGGTGTGAAA CGTCTGGATT CCAGTGCGTG GAAATGCTGA CAAACAAACC ATGGAACTAC 
CAGACAGACT ACTTTGGGAT CGCAGCCACC GTGTACTGCC TGCTCTTCGG GACCTACATG 
CGCCTCAAGG ATGACAATGG GGTCTGGAAG CCTGAGGGAT CCTTCAGGAG GCTTGCCAAT 
GCTGACCTGT GGAAAGAGTT CTTTGAGAGC TTGCTGAACA TTCCCAGCTG CCACAACCTG 
CCTTCCCTCG TAGTCCTGCG CCAGAAGCTC AAGGATTTAT TTTGCAGGTC CTACGCGAAG 
GAAATCAAGT TCCTTCGGAA CAGACTCGTC GTGCTGCTCA TCGAGCACAA ACGCTCACGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019143510.2
CDS	82..3147
Translation	MVCRYLQWAEECLPLQEKQSRWPGLLEELVKVFVGDKRYHQDLRFVSYCVKLAEFISSPCQYFEYLHGQGIGAKTSDFYLAWAQLLLREGNVQGAGAVLQKGLLNQAQPREHLQQLHCSLQSYDPRNPPLQDAAVVKPLQTSHAANQMAPPKGVSSPNPCKNQGLDAAGSQSCAVGKEVNYVTYISKSEVVPKAPSGATGWEQVPMYDKNLLLCEGSELSFEELRAKRYFRKYELLRRQQELEEEKKDSMKKKESAVLELQALQQKLDQLTQLSRSLEETRLEPAAEPNQRLPAKPLGDCTPAASPWEAPEEKDPAGAQAELGELQKSLLHPPFSAAPAQERAHPDPALPRSTGELSPNKGSTGLTPAVHVKEASRAGNSSFASGNISQATPNTSLGGAMGTTTPFKVQPSPTVHTKEALGVLMDMFQTPFLPEPSALEDSEEQFEAFCRRREPGGCLKTNIVVPVTPAFAIFEDEEEKENSRIPQHKNKPEEPRTFGERPLTDCAAEEETGTTEFLRDDYTVWNGPCRNKALAPSPDNTRDFARAAQLMSTPFNYLAAPSRPPLEDRACEENLPQMDLEFSGELHRQPKAKKLRPVLEHIPEQRELHGNLLKQGTGIQGIAAAASQRLHEETAPSRTGYSSSLGVDRTCQERLCGAGQDRTARTGRPAVLVENPWDTELVGKFLSELPKPLHTYANYFEWKSPLPFIKLKAELSLGSSSFHMDCLVGEGAFAQVYQASILDASNPRNNQKVIFKVQKPANPWEFYIASQLLERLSPSVRHLYIHFYSAHFFSNGSILVGELHNYGTLLNAVNIYKKLPEKVMPQALVIYFAVKILHMVEELHSCKIIHGDIKPDNFILGERFLDNDTCDIDGLCHGLTLIDLGQSIDMKLFPEGTAFTARCETSGFQCVEMLTNKPWNYQTDYFGIAATVYCLLFGTYMRLKDDNGVWKPEGSFRRLANADLWKEFFESLLNIPSCHNLPSLVVLRQKLKDLFCRSYAKEIKFLRNRLVVLLIEHKRSRK

Target ORF information:

RefSeq Version	XM_019287965.2
Organism	Corvus cornix cornix
Definition	Corvus cornix cornix BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019287965.2

ORF Insert Sequence:

ATGGTTTGCA GGTACCTGCA GTGGGCAGAG GAATGTCTCC CGCTCCAAGA GAAGCAGAGC 
CGCTGGCCCG GGCTCCTGGA GGAGCTGGTG AAGGTGTTCG TGGGCGACAA GAGGTACCAC 
CAGGACCTGC GGTTTGTGAG CTACTGCGTC AAGCTGGCAG AGTTCATCTC TTCCCCTTGC 
CAGTACTTTG AGTACTTGCA CGGGCAGGGA ATCGGAGCCA AGACCTCAGA TTTCTATCTG 
GCCTGGGCTC AGCTGCTCCT GAGGGAAGGC AACGTGCAGG GAGCAGGAGC TGTGCTCCAG 
AAAGGGCTCC TCAACCAGGC ACAGCCCCGG GAGCACCTGC AGCAGCTCCA CTGCTCCCTG 
CAGAGTTATG ATCCTCGGAA TCCTCCTTTG CAAGATGCTG CTGTTGTAAA ACCACTTCAG 
ACTTCCCATG CTGCAAATCA AATGGCTCCC CCAAAAGGTG TTTCAAGTCC TAATCCCTGC 
AAAAATCAGG GTCTGGATGC TGCTGGTTCC CAGTCCTGTG CTGTTGGAAA AGAAGTGAAC 
TACGTGACCT ACATCTCCAA GTCCGAGGTT GTGCCGAAGG CGCCGTCGGG TGCCACGGGG 
TGGGAGCAGG TCCCGATGTA CGACAAGAAC CTGCTCCTGT GCGAAGGCTC TGAGCTCTCC 
TTCGAGGAAC TGAGGGCCAA GAGATACTTC AGGAAATATG AGCTCCTCAG GAGGCAGCAG 
GAACTGGAGG AGGAGAAGAA AGACTCCATG AAGAAAAAGG AGTCGGCAGT CCTGGAGCTC 
CAAGCCCTGC AGCAGAAGCT GGACCAGCTG ACCCAGCTCT CCAGAAGCCT AGAGGAAACG 
AGACTGGAAC CAGCAGCTGA GCCAAACCAG AGGCTGCCAG CAAAGCCTCT CGGTGACTGC 
ACTCCAGCTG CCTCCCCCTG GGAAGCACCA GAGGAGAAGG ACCCAGCTGG AGCTCAGGCA 
GAGCTTGGAG AGCTCCAGAA GAGTTTGCTG CACCCTCCCT TCAGCGCTGC TCCAGCTCAG 
GAACGGGCTC ATCCCGACCC AGCTCTGCCT CGGAGCACGG GAGAACTGTC CCCTAACAAG 
GGATCCACTG GATTAACTCC TGCTGTGCAT GTGAAGGAAG CCTCCAGAGC TGGGAATTCC 
TCCTTTGCCT CTGGAAATAT TTCCCAAGCC ACCCCCAACA CCTCACTGGG AGGAGCAATG 
GGCACAACAA CGCCCTTCAA GGTGCAGCCG TCGCCCACGG TCCACACGAA AGAAGCCTTG 
GGTGTTCTCA TGGATATGTT CCAAACACCA TTCTTGCCTG AGCCATCTGC CCTGGAAGAC 
AGCGAGGAAC AATTTGAAGC CTTTTGCAGA AGAAGAGAGC CTGGTGGATG TTTGAAAACC 
AACATCGTTG TCCCTGTCAC TCCTGCATTT GCCATCTTTG AGGATGAGGA GGAGAAGGAG 
AACAGCAGGA TCCCACAGCA CAAGAACAAA CCAGAAGAGC CCAGAACTTT TGGAGAACGT 
CCCTTGACCG ACTGTGCAGC AGAAGAAGAA ACGGGAACCA CGGAATTCCT GCGGGATGAT 
TACACGGTGT GGAATGGCCC CTGCAGAAAC AAAGCCCTGG CTCCCAGTCC AGACAACACC 
AGGGACTTTG CCCGAGCTGC CCAGCTCATG TCCACACCCT TTAATTACCT GGCAGCTCCT 
TCGAGGCCCC CTTTGGAGGA CAGAGCCTGT GAGGAAAATT TGCCTCAAAT GGATTTGGAG 
TTCTCTGGAG AACTACACAG GCAGCCAAAG GCCAAAAAGT TAAGGCCTGT TCTTGAACAC 
ATCCCTGAAC AGAGAGAGCT TCATGGAAAC CTTCTGAAAC AAGGGACTGG AATCCAGGGA 
ATTGCTGCAG CTGCTTCCCA ACGACTGCAT GAGGAGACAG CTCCAAGCAG AACTGGATAT 
TCCTCCTCCC TTGGAGTGGA TAGAACTTGC CAGGAGAGGC TGTGTGGAGC TGGGCAGGAC 
AGGACAGCCC GGACTGGGAG ACCTGCAGTC CTTGTTGAGA ACCCTTGGGA CACGGAATTG 
GTTGGCAAGT TCTTGTCGGA GCTTCCAAAA CCGCTCCACA CCTACGCCAA CTACTTTGAG 
TGGAAATCTC CTCTTCCATT CATCAAACTG AAGGCTGAAC TCTCACTGGG CTCCAGCTCA 
TTCCACATGG ATTGCTTGGT CGGAGAGGGA GCTTTTGCCC AAGTCTATCA AGCTTCCATC 
CTGGATGCCA GCAACCCTCG GAACAATCAG AAAGTGATAT TCAAGGTCCA GAAACCTGCC 
AACCCTTGGG AGTTCTACAT TGCCAGCCAG CTGCTGGAGA GGCTGAGCCC CAGCGTGCGC 
CACCTCTACA TCCACTTCTA TTCCGCCCAT TTCTTCTCCA ACGGCAGCAT TTTGGTGGGG 
GAGCTCCACA ACTACGGAAC GCTGCTCAAT GCCGTCAACA TTTACAAGAA GCTCCCGGAG 
AAGGTGATGC CCCAAGCCCT CGTCATCTAC TTTGCTGTCA AAATCCTTCA CATGGTGGAG 
GAGCTGCACA GCTGCAAAAT CATCCATGGG GACATCAAAC CTGACAACTT CATCCTGGGA 
GAAAGGTTCC TGGACAACGA CACGTGTGAC ATTGATGGGC TCTGCCATGG CCTGACCCTC 
ATTGACCTGG GCCAGAGCAT CGACATGAAG CTCTTCCCTG AGGGAACAGC CTTCACTGCC 
AGGTGTGAAA CGTCTGGATT CCAGTGCGTG GAAATGCTGA CAAACAAACC ATGGAACTAC 
CAGACAGACT ACTTTGGGAT CGCAGCCACC GTGTACTGCC TGCTCTTCGG GACCTACATG 
CGCCTCAAGG ATGACAATGG GGTCTGGAAG CCTGAGGGAT CCTTCAGGAG GCTTGCCAAT 
GCTGACCTGT GGAAAGAGTT CTTTGAGAGC TTGCTGAACA TTCCCAGCTG CCACAACCTG 
CCTTCCCTCG TAGTCCTGCG CCAGAAGCTC AAGGATTTAT TTTGCAGGTC CTACGCGAAG 
GAAATCAAGT TCCTTCGGAA CAGACTCGTC GTGCTGCTCA TCGAGCACAA ACGCTCACGG 
AAATAA