CERK cDNA ORF clone, Corvus brachyrhynchos(American crow)

Gene
Clones
gRNAs

Summary

Gene Symbol	CERK
Entrez Gene ID	103623486
Full Name	ceramide kinase
Gene Type	protein-coding
Organism	Corvus brachyrhynchos(American crow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008642585.2	XP_008640807.1	ceramide kinase

Pathways from BioSystems
Gene Ontology

The following CERK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CERK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo55760	XM_008642585.2 Latest version!	Corvus brachyrhynchos ceramide kinase (CERK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CERK ( XM_008642585.2 ) cDNA ORF clone, Corvus brachyrhynchos(American crow) -> XP_008640807.1 Corvus brachyrhynchos ceramide kinase (CERK), mRNA.

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CloneID	OCo55760
Clone ID Related Accession (Same CDS sequence)	XM_008642585.2
Accession Version	XM_008642585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-02
Organism	Corvus brachyrhynchos(American crow)
Product	ceramide kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008246057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 3, 2016 this sequence version replaced XM_008642585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus brachyrhynchos Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGGAGAAGC GTCTAGTTTA CCAAACCGAG TGTGAACCTG CCCTCAAGCA TGTTCTGACT 
GTCAGAATTT TGGGAGTGGT GGAAGAAGAG TGGGATACAG TGAAGGCAAG TCTTAAACCT 
GAAAGCGTGA CCCTTCATGT GGAAAACTGT TTTGTGCCAA TCTCCGAGAT CATTGCTGTA 
GAAGAAACTG AGTTCAACAA GAAACAACGT AACATTGGCA GATGGCACAA AATGGCAAAG 
CTACATGCTT TCACGGTGTA TTATGTGAAG AAAGCCCGAA ATCACCGCTG GCGGTGCAGT 
GATATGACAT TTTGGTGTGT TGATGAGCCC ATATGTAATC AGTGGATACA GGCACTGAAA 
GAATTACTTG AAATGCAGAA GTCTAGACCA AAGCATTTGC TTGTGTATAT TAATCCCTAT 
GGAGGAAAAA GACAAGGGAA GAGGATTTAT GAACAGAAAG TTGCTCCACT CTTCAGTATG 
GCTTCTATAT CCACTGATGT TGTTATAACT GAACATGCTA ACCATGCCAA GGACAACTTA 
TTTGAAGTTA ATATTTATAA ATATGATGGT GTTGTTTGTG TTGGTGGGGA CGGCATGTTC 
AGTGAAGTGA TGCATGGTCT CATTGGAAGA ATGCAGAAGG ACTCTGGCAT AGACCAAAAT 
AATCCCAAAG CATCGTTAGT CCAGTGCAAT ATAAGGATTG GTCTGCTGCC ATGTGGATCA 
ACAGATTGCA TATGCTATTC AACTGTAGGC ATTTCTGATC CAGTAACTTC AGCTCTTCAT 
ATTATTGTAG GTGACTGTCA GCCTCTGGAT GTCTCTTCTG TACATCACAA CAACACGTTT 
TTGAAATACT CCGTGTCATT GCTGGGTTAT GGTTTTTATG GAGATATATT AAAAGATAGT 
GAGAAGAAGC GGTGGATGGG TCCAATGAGA TATGACTACT CAGGCTTCAA GACTTTTCTT 
TCTCATCATT ATTATGAAGG AACAATTGCT TATCAACCAG CAAAACATAC ACACGGATCT 
CCACGAGATA AAGAGAGCTG CAGAACAGGA TGTTACATTT GCAAGAAAAG TGAGAAACAG 
CTGGCAGAAC AGCACAAGGA ATGTGGATTA AAACATGAAG AAGGTGAAGA AGAGTGGAAG 
GTTGTAAAAG GGAAATTTCT AGCCATCAAT GCAGTAAGTA TGAGCTGTGC CTGTCCACGA 
AGTCCAAAAG GTCTTTCACC AGCAGCTCAT TTGGCAGATG GTTCAGCANN NNNNNNNNNN 
NNNNNNNNNT CCAGATTTGA TTTTCTACGG TATCTTGTCA GACACACAAA CCAAGATGAT 
CAGTTTGACT TTCCATTTGT AGACGTTTAT CGGGTGAAGT GTTTTCAATT TACATCAAAG 
CTTTCAGAAG ACAATGAAAG CAATGTCATG GACATAGGAA AGAAACGTTT TGGCCAGTTC 
TGCAGAGATC ATCCAGCCTG CTGCTGTAAT ATTGTTAATA GCACCTGGAA CTGTGATGGA 
GAAACTCTGG ATAGTTCAGC AATTGAAGTG AGGGTTCACT GCCAGTTAAT GAAACTGTTT 
GCAAGGGGAA TTGAGGAAAA CTGTAAAGAT GAAGCTGCCT ACAGCCAGAG TAGTGTTGAA 
CAATTACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008640807.1
CDS	1..1632
Translation	MEKRLVYQTECEPALKHVLTVRILGVVEEEWDTVKASLKPESVTLHVENCFVPISEIIAVEETEFNKKQRNIGRWHKMAKLHAFTVYYVKKARNHRWRCSDMTFWCVDEPICNQWIQALKELLEMQKSRPKHLLVYINPYGGKRQGKRIYEQKVAPLFSMASISTDVVITEHANHAKDNLFEVNIYKYDGVVCVGGDGMFSEVMHGLIGRMQKDSGIDQNNPKASLVQCNIRIGLLPCGSTDCICYSTVGISDPVTSALHIIVGDCQPLDVSSVHHNNTFLKYSVSLLGYGFYGDILKDSEKKRWMGPMRYDYSGFKTFLSHHYYEGTIAYQPAKHTHGSPRDKESCRTGCYICKKSEKQLAEQHKECGLKHEEGEEEWKVVKGKFLAINAVSMSCACPRSPKGLSPAAHLADGSAnullnullnullnullnullnullnullSRFDFLRYLVRHTNQDDQFDFPFVDVYRVKCFQFTSKLSEDNESNVMDIGKKRFGQFCRDHPACCCNIVNSTWNCDGETLDSSAIEVRVHCQLMKLFARGIEENCKDEAAYSQSSVEQLL

Target ORF information:

RefSeq Version	XM_008642585.2
Organism	Corvus brachyrhynchos(American crow)
Definition	Corvus brachyrhynchos ceramide kinase (CERK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008642585.2

ORF Insert Sequence:

ATGGAGAAGC GTCTAGTTTA CCAAACCGAG TGTGAACCTG CCCTCAAGCA TGTTCTGACT 
GTCAGAATTT TGGGAGTGGT GGAAGAAGAG TGGGATACAG TGAAGGCAAG TCTTAAACCT 
GAAAGCGTGA CCCTTCATGT GGAAAACTGT TTTGTGCCAA TCTCCGAGAT CATTGCTGTA 
GAAGAAACTG AGTTCAACAA GAAACAACGT AACATTGGCA GATGGCACAA AATGGCAAAG 
CTACATGCTT TCACGGTGTA TTATGTGAAG AAAGCCCGAA ATCACCGCTG GCGGTGCAGT 
GATATGACAT TTTGGTGTGT TGATGAGCCC ATATGTAATC AGTGGATACA GGCACTGAAA 
GAATTACTTG AAATGCAGAA GTCTAGACCA AAGCATTTGC TTGTGTATAT TAATCCCTAT 
GGAGGAAAAA GACAAGGGAA GAGGATTTAT GAACAGAAAG TTGCTCCACT CTTCAGTATG 
GCTTCTATAT CCACTGATGT TGTTATAACT GAACATGCTA ACCATGCCAA GGACAACTTA 
TTTGAAGTTA ATATTTATAA ATATGATGGT GTTGTTTGTG TTGGTGGGGA CGGCATGTTC 
AGTGAAGTGA TGCATGGTCT CATTGGAAGA ATGCAGAAGG ACTCTGGCAT AGACCAAAAT 
AATCCCAAAG CATCGTTAGT CCAGTGCAAT ATAAGGATTG GTCTGCTGCC ATGTGGATCA 
ACAGATTGCA TATGCTATTC AACTGTAGGC ATTTCTGATC CAGTAACTTC AGCTCTTCAT 
ATTATTGTAG GTGACTGTCA GCCTCTGGAT GTCTCTTCTG TACATCACAA CAACACGTTT 
TTGAAATACT CCGTGTCATT GCTGGGTTAT GGTTTTTATG GAGATATATT AAAAGATAGT 
GAGAAGAAGC GGTGGATGGG TCCAATGAGA TATGACTACT CAGGCTTCAA GACTTTTCTT 
TCTCATCATT ATTATGAAGG AACAATTGCT TATCAACCAG CAAAACATAC ACACGGATCT 
CCACGAGATA AAGAGAGCTG CAGAACAGGA TGTTACATTT GCAAGAAAAG TGAGAAACAG 
CTGGCAGAAC AGCACAAGGA ATGTGGATTA AAACATGAAG AAGGTGAAGA AGAGTGGAAG 
GTTGTAAAAG GGAAATTTCT AGCCATCAAT GCAGTAAGTA TGAGCTGTGC CTGTCCACGA 
AGTCCAAAAG GTCTTTCACC AGCAGCTCAT TTGGCAGATG GTTCAGCANN NNNNNNNNNN 
NNNNNNNNNT CCAGATTTGA TTTTCTACGG TATCTTGTCA GACACACAAA CCAAGATGAT 
CAGTTTGACT TTCCATTTGT AGACGTTTAT CGGGTGAAGT GTTTTCAATT TACATCAAAG 
CTTTCAGAAG ACAATGAAAG CAATGTCATG GACATAGGAA AGAAACGTTT TGGCCAGTTC 
TGCAGAGATC ATCCAGCCTG CTGCTGTAAT ATTGTTAATA GCACCTGGAA CTGTGATGGA 
GAAACTCTGG ATAGTTCAGC AATTGAAGTG AGGGTTCACT GCCAGTTAAT GAAACTGTTT 
GCAAGGGGAA TTGAGGAAAA CTGTAAAGAT GAAGCTGCCT ACAGCCAGAG TAGTGTTGAA 
CAATTACTAT AA