NELFE cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFE
Entrez Gene ID	101631240
Full Name	negative elongation factor complex member E
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004695152.2	XP_004695209.1	negative elongation factor E

Pathways from BioSystems
Gene Ontology

The following NELFE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo247380	XM_004695152.2 Latest version!	Condylura cristata negative elongation factor complex member E (NELFE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFE ( XM_004695152.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004695209.1 Condylura cristata negative elongation factor complex member E (NELFE), mRNA.

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CloneID	OCo247380
Clone ID Related Accession (Same CDS sequence)	XM_004695152.2
Accession Version	XM_004695152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	negative elongation factor E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567156.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004695152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAGGAAGAGG CTCTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCATTACTG GCTCTGAAGA AACAAAGTAG CAGCAGCACA 
GCCAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA CACAGCCACA 
GCAACAGAGC AAGCAAAGCA GCTGGTCAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA ACGTTCTCGA ACCTTAGAGG GGAAGTTAAA GGACCCAGAG 
AAGGGCCCAG TCCCCACTTT CCAGCCATTC CAAAGGAGCA TATCTGCCGA TGATGATCTG 
CAGGAGTCAT CCAGACGTCC CCAGAGGAAG TCTCTGTATG AGAGTTTTGT GTCTTCCAGT 
GATCGACTTC GAGAACTGGG GCCAGATGGA GAAGAAGCAG AGGGCCCAGG GGCCGGTGAT 
GGCCCTCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGTG TTGGTTCCCG CTCCTCAGCC 
TCCCCTCCCC AAAGCCGCAG CCGGGACCGC AGTAGAGAAC GGAATCGGGA CAGAGACCGA 
GATCGGGAAC GGGATCGGGA TCGGGATCGG GATAGAGACA GAGATCGAGA CCGGGACCGG 
GACCGGGACA GAGACAGAGA CCGGGACCGG GACCGAGACC GAGATCGGGA CAGGGATCGA 
GACAGGGATC GGGATCGAGA TCGAGATCGA GAGGGCCCTT TCCGCAGGTC GGACTCATTC 
CCTGAACGCC GAGCCCCTAG GAAGGGGAAT ACTCTGTATG TGTACGGAGA AGACATGACG 
CCCACTCTCC TCCGTGGGGC CTTCTCCCCC TTTGGAAACA TCATTGACCT CTCCATGGAC 
CCACCCAGAA ACTGTGCCTT TGTCACCTAT GAAAAGATGG AGTCTGCAGA TCAGGCCGTT 
GCTGAGCTCA ATGGGACCCA GGTGGATTCC GTGCAGCTCA AAGTCAGCAT TGCCCGAAAA 
CAGCCCATGC TGGACGTCGC CACTGGCAAG TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC 
AGCCCTAAGG GTTGCCACCG GGACAAGAGA ACCCAGATTG TCTACAGTGA TGACATCTAC 
AAGGAAAACC TTGTGGATGG CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004695209.1
CDS	152..1318
Translation	MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTASQGGVKRSLSEQPVVDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERVGSRSSASPPQSRSRDRSRERNRDRDRDRERDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVDSVQLKVSIARKQPMLDVATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDIYKENLVDGF

Target ORF information:

RefSeq Version	XM_004695152.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata negative elongation factor complex member E (NELFE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004695152.2

ORF Insert Sequence:

ATGTTGGTGA TACCCCCCGG ACTGAGCGAG GAGGAAGAGG CTCTACAGAA GAAATTCAAC 
AAACTCAAGA AAAAGAAAAA GGCATTACTG GCTCTGAAGA AACAAAGTAG CAGCAGCACA 
GCCAGCCAGG GCGGTGTCAA GCGCTCACTG TCAGAGCAGC CTGTGGTGGA CACAGCCACA 
GCAACAGAGC AAGCAAAGCA GCTGGTCAAG TCAGGAGCCA TCAGTGCCAT CAAGGCTGAG 
ACCAAGAACT CAGGCTTCAA ACGTTCTCGA ACCTTAGAGG GGAAGTTAAA GGACCCAGAG 
AAGGGCCCAG TCCCCACTTT CCAGCCATTC CAAAGGAGCA TATCTGCCGA TGATGATCTG 
CAGGAGTCAT CCAGACGTCC CCAGAGGAAG TCTCTGTATG AGAGTTTTGT GTCTTCCAGT 
GATCGACTTC GAGAACTGGG GCCAGATGGA GAAGAAGCAG AGGGCCCAGG GGCCGGTGAT 
GGCCCTCCTC GAAGCTTTGA CTGGGGCTAT GAAGAACGTG TTGGTTCCCG CTCCTCAGCC 
TCCCCTCCCC AAAGCCGCAG CCGGGACCGC AGTAGAGAAC GGAATCGGGA CAGAGACCGA 
GATCGGGAAC GGGATCGGGA TCGGGATCGG GATAGAGACA GAGATCGAGA CCGGGACCGG 
GACCGGGACA GAGACAGAGA CCGGGACCGG GACCGAGACC GAGATCGGGA CAGGGATCGA 
GACAGGGATC GGGATCGAGA TCGAGATCGA GAGGGCCCTT TCCGCAGGTC GGACTCATTC 
CCTGAACGCC GAGCCCCTAG GAAGGGGAAT ACTCTGTATG TGTACGGAGA AGACATGACG 
CCCACTCTCC TCCGTGGGGC CTTCTCCCCC TTTGGAAACA TCATTGACCT CTCCATGGAC 
CCACCCAGAA ACTGTGCCTT TGTCACCTAT GAAAAGATGG AGTCTGCAGA TCAGGCCGTT 
GCTGAGCTCA ATGGGACCCA GGTGGATTCC GTGCAGCTCA AAGTCAGCAT TGCCCGAAAA 
CAGCCCATGC TGGACGTCGC CACTGGCAAG TCTGTCTGGG GCTCCCTTGC TGTCCAGAAC 
AGCCCTAAGG GTTGCCACCG GGACAAGAGA ACCCAGATTG TCTACAGTGA TGACATCTAC 
AAGGAAAACC TTGTGGATGG CTTCTAG