AGO1 cDNA ORF clone, Chlamydomonas reinhardtii

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO1
Entrez Gene ID	5720425
Full Name	argonaute protein
Gene Type	protein-coding
Organism	Chlamydomonas reinhardtii

mRNA and Protein(s)

mRNA	Protein	Name
XM_001694789.1	XP_001694841.1	argonaute protein
XM_001694788.1	XP_001694840.1	argonaute-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Mar?chal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fern?ndez E, Fukuzawa H, Gonz?lez-Ballester D, Gonz?lez-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Ria?o-Pach?n DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Mart?nez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
Science (New York, N.Y.)318(5848)245-50(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh03489	XM_001694788.1 Latest version!	Chlamydomonas reinhardtii argonaute-like protein (AGO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCh03490	XM_001694789.1 Latest version!	Chlamydomonas reinhardtii argonaute protein (AGO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO1 ( XM_001694788.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001694840.1 Chlamydomonas reinhardtii argonaute-like protein (AGO1), mRNA.

Price & Availability↑

CloneID	OCh03489
Clone ID Related Accession (Same CDS sequence)	XM_001694788.1
Accession Version	XM_001694788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-28
Organism	Chlamydomonas reinhardtii
Product	argonaute-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843688).

ATGCTGAGCA AGGTCCAGTT GCCTCGACGG GCTGGCCGCG CGAACAGCGC AACGCCTGTG 
TCAGCTCGGT ACTCGGACTC GGCCAACCGG CCAGGAGGTC GCGGAGGCGG CCAACCGGGC 
CGGGGAGGTG GAGGCAGGGG AGGTGGCTCA GGCAGGGGCT CATCGTCGTC GACCGCGCCG 
AAGGAGCTCC TCACCCCGGC CCAGTACTGG CCGTCGTTTT GGACCCGGCT GTTCACCGGT 
CTTCCGCTGG GGCCAGTGGA GCCGCGCCAG TTGGCTAATG GACATGCGGC GCGGCCGGGT 
TACAGCGCCG CGGGCAAGAC CATCCAGGTG CTCACCAATC ACGTGCCGCT CAATGTGGGC 
GTCCGCACCG TGCATCAGTA CGACGTGCGC ATCAGCCGCG TCGCCAACAG TGCCCCGACC 
TCCTCAGCAG CAGCACCTTC GGGGCAGGGG GGCGGCAAGC CCGGCCGCGG CCGCAAGGAG 
GCCAAGGTGT CGGGGCAGGA GGCGCAGGCG GTCCTGGTGG CGCTGCTGGG CGACACAGGC 
GCAGGGGGCT CTGGAGCGGC CGGGGGCGTC TGGGTGTTCG ACGGCAGCCA GGCGCTGTAC 
GGCGTGGAGG GCGACCTCAG GCGCAGGGCG GCCGCAGCTG CGGCGGGCGC CGGCGGGGCC 
GGGGGCGATG GGGAGCTGCA GGTTCAGCTG GCAGCGGCGC CCACGACTGG TGTTGTCGGC 
GGCACATACA AGGTGGACCT GAAGCTGGTG GGGGCGCTGG ACGTGGCAGA GTGCCTGAGG 
GAGGCGGCGC AGATGCCGGG GCCGACAACA CGCCGGCCGC CGCCCGACGA GGAACAGCCC 
GAGGGCTCGC CCGCGCCAGC GGGTGTGCTG CCGCCGCCGC CCGCCCCCCC AGCCACGTCC 
GGCGCCGCCG CACTGGCGGT GTTGGACCTT GTGACTCGGG CGGGGGTTGC GTGGGACGTG 
GCCTCGGTGC GCACGGGCGG GGGCTCCTAC GTGTCCTACA AGCAGGAGGA CGCCAGCGGG 
CCGGTGAAGG GCCTGCTGCC AATGAAGCTG CTGCGCGGCT TCCGCTCCGC TGTGGCTCAG 
GTGGCGGCCG GCACCACCCT CACCATTGAT GCCGCGAGTG CGGTGGTGGC GGGCGACGGC 
CCCCTGCTCG GCCAACTGCA GGCTGCGCTA GCGGCGGGAC GGGACGTGTC GGCGCTGGCG 
ACGGAGCTGG GCAACAGCAA GAACGCCCGG CGCCTGGAGG AGGCGCTGGT GGGGCTGGAG 
GTCCAGCCCA GCTACACCGC CAACCGCTAC AAACTGTCCG GCAAGTTGGG CAAGTCCGCC 
CGCAGCCTCG CATTTGAGTT CGAGGGCCGC AAGATCAGCG TCCAGACTTA CTTCAAGGAG 
CGGTACGGCG TGTCGCTGCA GCACCCCGAC CTGCCCTGTG TGCTGGACAA GAAGGGCGGC 
GCACTGCCCA TTGAGCTGCT CACTGTGGTC AAGTACCAGA AGCGCATGTC GCGGCTTGAC 
GCCCGCCAGA AGGCCGACTA CATCCGTACA GCCGCCCTCA AGCCGGCAGA CCGCATGAGG 
GCCATGCGGG AGGCACTGAC GCGGCAGCTG GGCTCGGTGC GGGGTCAGGT GGCGCGCGCG 
TTTGGCCTGG CGCCGTTCAA CGACATGCCC GACTTCCTCA AGGTGCCGGC GCAGCTGCTG 
CCTGGCCCGC TGCTGCAGGT GGCCAAGCAG CAGGCGGTGG AGCCCGGCAA CAAGGGCGAA 
TGGGCCGGCG GCGGCGTGGC GGTGGCGCCG GAGTGGCGCA GCGGCGCCGT GCTGTGCTAC 
CTGAGCCAGT CGGAGGGGCA GGCCCCGCTG TCGGGCCTGC TGGCCGCTCT GTCCAAGGCA 
CTGTCCGCCT CGTCCGCCAG CGCCCTGCCG CCCAACATCC CCGTCATATG GGCCTCGCTG 
CCCAAGCCCG GCATGTCGCC CGACAAGGCC GCTGCGGACG CGGAGTTCTG GCTCAACCGC 
GCGGCGGCCA AGGCTCAAGA GCAGTACAAG CGGCAGGCGC AGATGGTGAT GGTGGTGCTG 
CCGGACAGGG GCGGCGTAGG CCTGTATGAC GCCATCAAGG CGGCAGGCGA CGGCAAGTTG 
GGAGTGGCCA CCCAGTGCGT CAACCCGGCC AAGGCGGGAT TGGTGGGCCC CAAGGCCTCC 
TCCGGCCCCT CCTTCTCCTA CGCGGCCCAG TTGGCGCTGT CGGTCAACGC CAAACTGGGC 
GGCGCCACCA CGAGGCCGGC AGGCAGGCCC AATGGTGAGC TCGCTCTCGC CCTCCCCCGC 
ACCCGGCGCA CACGAGTGCA GGTGTCGGCT GGAGTCGAAT ACGCTGCTGT TGTGGGGTCG 
GCAGACAGCC ACGCGGTCGA CTACCGAGTG CAGCTGAGTG CGCAGGTGGC GGGCAACCGC 
GACATAGTTG TCAGCATGCG CGAGAGTGTG CGGCGGCTGC TGCTGCAGTA CGGCTTGCCT 
CAGGGCGGGG CGGTCGCGGC CGGCGGCCCC CGGCCCTCCT CCGAGCTGCC AGAGGTGGTG 
CTCATGTACC GGGACGGGCT GAGTGAGAGC CAGTTCGACC GGGCCCTGGC GGAGGAGTTT 
ACCGCCATTG TGCAGGCCTG CGCCGACGTG GGCGGTCCGT CCTACCGGCC GCGCATCTGC 
TTCGTGGTGG TCCAGAAGTC CCACAACACC CGCTTCTTCC CCACCTCGGC CGAGTCCACG 
CACAAGAGCG GCAACGTGCT GCCGGGCACC GCTGTGGACC GAGGCGTGAC GGACCCCCAC 
GCCTTCGACT TCTTCCTCAA CTCGCACGCC GGCATACAGG GCACCAACCG GGCGCCCAGG 
TACACTGTGC TTGTGGACGA GGTGGGCTTC ACGGCGGAGG CGCTGCAGCT GCTGACCCAC 
ACGCTGTGCC ACACATACCC GGCCTGCACC AGGGCGCTCT CGCTGCCGCC GCCTGTGCGC 
TACGCCGACC GGGCGGCGGA CCGCGCACGC ACACTGCGGT ACATGGTGCC GCGGCCGTCT 
GGCAAGCAGG GCCGCGGCGT GGACTGGTAC CAGATGCTGC CGACGCTGCC GCACGCAGGG 
CACGTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001694840.1
CDS	122..3193
Translation	MLSKVQLPRRAGRANSATPVSARYSDSANRPGGRGGGQPGRGGGGRGGGSGRGSSSSTAPKELLTPAQYWPSFWTRLFTGLPLGPVEPRQLANGHAARPGYSAAGKTIQVLTNHVPLNVGVRTVHQYDVRISRVANSAPTSSAAAPSGQGGGKPGRGRKEAKVSGQEAQAVLVALLGDTGAGGSGAAGGVWVFDGSQALYGVEGDLRRRAAAAAAGAGGAGGDGELQVQLAAAPTTGVVGGTYKVDLKLVGALDVAECLREAAQMPGPTTRRPPPDEEQPEGSPAPAGVLPPPPAPPATSGAAALAVLDLVTRAGVAWDVASVRTGGGSYVSYKQEDASGPVKGLLPMKLLRGFRSAVAQVAAGTTLTIDAASAVVAGDGPLLGQLQAALAAGRDVSALATELGNSKNARRLEEALVGLEVQPSYTANRYKLSGKLGKSARSLAFEFEGRKISVQTYFKERYGVSLQHPDLPCVLDKKGGALPIELLTVVKYQKRMSRLDARQKADYIRTAALKPADRMRAMREALTRQLGSVRGQVARAFGLAPFNDMPDFLKVPAQLLPGPLLQVAKQQAVEPGNKGEWAGGGVAVAPEWRSGAVLCYLSQSEGQAPLSGLLAALSKALSASSASALPPNIPVIWASLPKPGMSPDKAAADAEFWLNRAAAKAQEQYKRQAQMVMVVLPDRGGVGLYDAIKAAGDGKLGVATQCVNPAKAGLVGPKASSGPSFSYAAQLALSVNAKLGGATTRPAGRPNGELALALPRTRRTRVQVSAGVEYAAVVGSADSHAVDYRVQLSAQVAGNRDIVVSMRESVRRLLLQYGLPQGGAVAAGGPRPSSELPEVVLMYRDGLSESQFDRALAEEFTAIVQACADVGGPSYRPRICFVVVQKSHNTRFFPTSAESTHKSGNVLPGTAVDRGVTDPHAFDFFLNSHAGIQGTNRAPRYTVLVDEVGFTAEALQLLTHTLCHTYPACTRALSLPPPVRYADRAADRARTLRYMVPRPSGKQGRGVDWYQMLPTLPHAGHVV

Target ORF information:

RefSeq Version	XM_001694788.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii argonaute-like protein (AGO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001694788.1

ORF Insert Sequence:

ATGCTGAGCA AGGTCCAGTT GCCTCGACGG GCTGGCCGCG CGAACAGCGC AACGCCTGTG 
TCAGCTCGGT ACTCGGACTC GGCCAACCGG CCAGGAGGTC GCGGAGGCGG CCAACCGGGC 
CGGGGAGGTG GAGGCAGGGG AGGTGGCTCA GGCAGGGGCT CATCGTCGTC GACCGCGCCG 
AAGGAGCTCC TCACCCCGGC CCAGTACTGG CCGTCGTTTT GGACCCGGCT GTTCACCGGT 
CTTCCGCTGG GGCCAGTGGA GCCGCGCCAG TTGGCTAATG GACATGCGGC GCGGCCGGGT 
TACAGCGCCG CGGGCAAGAC CATCCAGGTG CTCACCAATC ACGTGCCGCT CAATGTGGGC 
GTCCGCACCG TGCATCAGTA CGACGTGCGC ATCAGCCGCG TCGCCAACAG TGCCCCGACC 
TCCTCAGCAG CAGCACCTTC GGGGCAGGGG GGCGGCAAGC CCGGCCGCGG CCGCAAGGAG 
GCCAAGGTGT CGGGGCAGGA GGCGCAGGCG GTCCTGGTGG CGCTGCTGGG CGACACAGGC 
GCAGGGGGCT CTGGAGCGGC CGGGGGCGTC TGGGTGTTCG ACGGCAGCCA GGCGCTGTAC 
GGCGTGGAGG GCGACCTCAG GCGCAGGGCG GCCGCAGCTG CGGCGGGCGC CGGCGGGGCC 
GGGGGCGATG GGGAGCTGCA GGTTCAGCTG GCAGCGGCGC CCACGACTGG TGTTGTCGGC 
GGCACATACA AGGTGGACCT GAAGCTGGTG GGGGCGCTGG ACGTGGCAGA GTGCCTGAGG 
GAGGCGGCGC AGATGCCGGG GCCGACAACA CGCCGGCCGC CGCCCGACGA GGAACAGCCC 
GAGGGCTCGC CCGCGCCAGC GGGTGTGCTG CCGCCGCCGC CCGCCCCCCC AGCCACGTCC 
GGCGCCGCCG CACTGGCGGT GTTGGACCTT GTGACTCGGG CGGGGGTTGC GTGGGACGTG 
GCCTCGGTGC GCACGGGCGG GGGCTCCTAC GTGTCCTACA AGCAGGAGGA CGCCAGCGGG 
CCGGTGAAGG GCCTGCTGCC AATGAAGCTG CTGCGCGGCT TCCGCTCCGC TGTGGCTCAG 
GTGGCGGCCG GCACCACCCT CACCATTGAT GCCGCGAGTG CGGTGGTGGC GGGCGACGGC 
CCCCTGCTCG GCCAACTGCA GGCTGCGCTA GCGGCGGGAC GGGACGTGTC GGCGCTGGCG 
ACGGAGCTGG GCAACAGCAA GAACGCCCGG CGCCTGGAGG AGGCGCTGGT GGGGCTGGAG 
GTCCAGCCCA GCTACACCGC CAACCGCTAC AAACTGTCCG GCAAGTTGGG CAAGTCCGCC 
CGCAGCCTCG CATTTGAGTT CGAGGGCCGC AAGATCAGCG TCCAGACTTA CTTCAAGGAG 
CGGTACGGCG TGTCGCTGCA GCACCCCGAC CTGCCCTGTG TGCTGGACAA GAAGGGCGGC 
GCACTGCCCA TTGAGCTGCT CACTGTGGTC AAGTACCAGA AGCGCATGTC GCGGCTTGAC 
GCCCGCCAGA AGGCCGACTA CATCCGTACA GCCGCCCTCA AGCCGGCAGA CCGCATGAGG 
GCCATGCGGG AGGCACTGAC GCGGCAGCTG GGCTCGGTGC GGGGTCAGGT GGCGCGCGCG 
TTTGGCCTGG CGCCGTTCAA CGACATGCCC GACTTCCTCA AGGTGCCGGC GCAGCTGCTG 
CCTGGCCCGC TGCTGCAGGT GGCCAAGCAG CAGGCGGTGG AGCCCGGCAA CAAGGGCGAA 
TGGGCCGGCG GCGGCGTGGC GGTGGCGCCG GAGTGGCGCA GCGGCGCCGT GCTGTGCTAC 
CTGAGCCAGT CGGAGGGGCA GGCCCCGCTG TCGGGCCTGC TGGCCGCTCT GTCCAAGGCA 
CTGTCCGCCT CGTCCGCCAG CGCCCTGCCG CCCAACATCC CCGTCATATG GGCCTCGCTG 
CCCAAGCCCG GCATGTCGCC CGACAAGGCC GCTGCGGACG CGGAGTTCTG GCTCAACCGC 
GCGGCGGCCA AGGCTCAAGA GCAGTACAAG CGGCAGGCGC AGATGGTGAT GGTGGTGCTG 
CCGGACAGGG GCGGCGTAGG CCTGTATGAC GCCATCAAGG CGGCAGGCGA CGGCAAGTTG 
GGAGTGGCCA CCCAGTGCGT CAACCCGGCC AAGGCGGGAT TGGTGGGCCC CAAGGCCTCC 
TCCGGCCCCT CCTTCTCCTA CGCGGCCCAG TTGGCGCTGT CGGTCAACGC CAAACTGGGC 
GGCGCCACCA CGAGGCCGGC AGGCAGGCCC AATGGTGAGC TCGCTCTCGC CCTCCCCCGC 
ACCCGGCGCA CACGAGTGCA GGTGTCGGCT GGAGTCGAAT ACGCTGCTGT TGTGGGGTCG 
GCAGACAGCC ACGCGGTCGA CTACCGAGTG CAGCTGAGTG CGCAGGTGGC GGGCAACCGC 
GACATAGTTG TCAGCATGCG CGAGAGTGTG CGGCGGCTGC TGCTGCAGTA CGGCTTGCCT 
CAGGGCGGGG CGGTCGCGGC CGGCGGCCCC CGGCCCTCCT CCGAGCTGCC AGAGGTGGTG 
CTCATGTACC GGGACGGGCT GAGTGAGAGC CAGTTCGACC GGGCCCTGGC GGAGGAGTTT 
ACCGCCATTG TGCAGGCCTG CGCCGACGTG GGCGGTCCGT CCTACCGGCC GCGCATCTGC 
TTCGTGGTGG TCCAGAAGTC CCACAACACC CGCTTCTTCC CCACCTCGGC CGAGTCCACG 
CACAAGAGCG GCAACGTGCT GCCGGGCACC GCTGTGGACC GAGGCGTGAC GGACCCCCAC 
GCCTTCGACT TCTTCCTCAA CTCGCACGCC GGCATACAGG GCACCAACCG GGCGCCCAGG 
TACACTGTGC TTGTGGACGA GGTGGGCTTC ACGGCGGAGG CGCTGCAGCT GCTGACCCAC 
ACGCTGTGCC ACACATACCC GGCCTGCACC AGGGCGCTCT CGCTGCCGCC GCCTGTGCGC 
TACGCCGACC GGGCGGCGGA CCGCGCACGC ACACTGCGGT ACATGGTGCC GCGGCCGTCT 
GGCAAGCAGG GCCGCGGCGT GGACTGGTAC CAGATGCTGC CGACGCTGCC GCACGCAGGG 
CACGTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( XM_001694789.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001694841.1 Chlamydomonas reinhardtii argonaute protein (AGO1), mRNA.

Price & Availability↑

CloneID	OCh03490
Clone ID Related Accession (Same CDS sequence)	XM_001694789.1
Accession Version	XM_001694789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-28
Organism	Chlamydomonas reinhardtii
Product	argonaute protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843688).

ATGCTGAGCA AGGTCCAGTT GCCTCGACGG GCTGGCCGCG CGAACAGCGC AACGCCTGTG 
TCAGCTCGGT ACTCGGACTC GGCCAACCGG CCAGGAGGTC GCGGAGGCGG CCAACCGGGC 
CGGGGAGGTG GAGGCAGGGG AGGTGGCTCA GGCAGGGGCT CATCGTCGTC GACCGCGCCG 
AAGGAGCTCC TCACCCCGGC CCAGTACTGG CCGTCGTTTT GGACCCGGCT GTTCACCGGT 
CTTCCGCTGG GGCCAGTGGA GCCGCGCCAG TTGGCTAATG GACATGCGGC GCGGCCGGGT 
TACAGCGCCG CGGGCAAGAC CATCCAGGTG CTCACCAATC ACGTGCCGCT CAATGTGGGC 
GTCCGCACCG TGCATCAGTA CGACGTGCGC ATCAGCCGCG TCGCCAACAG TGCCCCGACC 
TCCTCAGCAG CAGCACCTTC GGGGCAGGGG GGCGGCAAGC CCGGCCGCGG CCGCAAGGAG 
GCCAAGGTGT CGGGGCAGGA GGCGCAGGCG GTCCTGGTGG CGCTGCTGGG CGACACAGGC 
GCAGGGGGCT CTGGAGCGGC CGGGGGCGTC TGGGTGTTCG ACGGCAGCCA GGCGCTGTAC 
GGCGTGGACC TGAAGCTGGT GGGGGCGCTG GACGTGGCAG AGTGCCTGAG GGAGGCGGCG 
CAGATGCCGG GGCCGACAAC ACGCCGGCCG CCGCCCGACG AGGAACAGCC CGAGGGCTCG 
CCCGCGCCAG CGGCCACGTC CGGCGCCGCC GCACTGGCGG TGTTGGACCT TGTGACTCGG 
GCGGGGGTTG CGTGGGACGT GGCCTCGGTG CGCACGGGCG GGGGCTCCTA CGTGTCCTAC 
AAGCAGGAGG ACGCCAGCGG GCCGGTGAAG GGCCTGCTGC CAATGAAGCT GCTGCGCGGC 
TTCCGCTCCG CTGTGGCTCA GGTGGCGGCC GGCACCACCC TCACCATTGA TGCCGCGAGT 
GCGGTGGTGG CGGGCGACGG CCCCCTGCTC GGCCAACTGC AGGCAGGCGC TGAGGCAGAG 
AAGACATCGG CTGCGCTAGC GGCGGGACGG GACGTGTCGG CGCTGGCGAC GGAGCTGGGC 
AACAGCAAGA ACGCCCGGCG CCTGGAGGAG GCGCTGGTGG GGCTGGAGGT CCAGCCCAGC 
TACACCGCCA ACCGCTACAA ACTGTCCGGC AAGTTGGGCA AGTCCGCCCG CAGCCTCGCA 
TTTGAGTTCG AGGGCCGCAA GATCAGCGTC CAGCACCCCG ACCTGCCCTG TGTGCTGGAC 
AAGAAGGGCG GCGCACTGCC CATTGAGCTG CTCACTGTGG TCAAGTACCA GAAGCGCATG 
TCGCGGCTTG ACGCCCGCCA GAAGGCCGAC TACATCCGTA CAGCCGCCCT CAAGCCGGCA 
GACCGCATGA GGGCCATGCG GGAGGCACTG ACGCGGCAGC TGGGCTCGGT GCGGGGTCAG 
GTGGCGCGCG CGTTTGGCCT GGCGCCGTTC AACGACATGC CCGACTTCCT CAAGGTGCCG 
GCGCAGCTGC TGCCTGGCCC GCTGCTGCAG GTGGCCAAGC AGCAGGCGGT GGAGCCCGGC 
AACAAGGGCG AATGGGCCGG CGGCGGCGTG GCGGTGGCGC CGGAGTGGCG CAGCGGCGCC 
GTGCTGTGCT ACCTGAGCCA GTCGGAGGGG CAGGCCCCGC TGTCGGGCCT GCTGGCCGCT 
CTGTCCAAGG CACTGTCCGC CTCGTCCGCC AGCGCCCTGC CGCCCAACAT CCCCGTCATA 
TGGGCCTCGC TGCCCAAGCC CGGCATGTCG CCCGACAAGG CCGCTGCGGA CGCGGAGTTC 
TGGCTCAACC GCGCGGCGGC CAAGGCTCAA GAGCAGTACA AGCGGCAGGC GCAGATGGTG 
ATGGTGGTGC TGCCGGACAG GGGCGGCGTA GGCCTGTATG ACGCCATCAA GGCGGCAGGC 
GACGGCAAGT TGGGAGTGGC CACCCAGTGC GTCAACCCGG CCAAGGCGGG ATTGGTGGGC 
CCCAAGGCCT CCTCCGGCCC CTCCTTCTCC TACGCGGCCC AGTTGGCGCT GTCGGTCAAC 
GCCAAACTGG GCGGCGCCAC CACGAGGCCG GCAGGCAGGC CCAATGACTG GCTGCCGCTG 
CTGGGCGGCC GGCGGCTGAT GGTGGGCGGG CGGGGGCCTG GACGGCTGGA GGCGCGGCTG 
GGGCTGAGTG GCGCACTGTT AAGTCGTGGC TATAGAGGTG CCGCCCAGGT GTCGGCTGGA 
GTCGAATACG CTGCTGTTGT GGGGTCGGCA GACAGCCACG CGGTCGACTA CCGAGTGCAG 
CTGAGTGCGC AGGTGGCGGG CAACCGCGAC ATAGTTGTCA GCATGCGCGA GAGTGTGGTG 
CTCATGTACC GGGACGGGCT GAGTGAGAGC CAGTTCGACC GGGCCCTGGC GGAGGAGTTT 
ACCGCCATTG TGCAGGCCTG CGCCGACGTG GGCGGTCCGT CCTACCGGCC GCGCATCTGC 
TTCGTGGTGG TCCAGAAGTC CCACAACACC CGCTTCTTCC CCACCTCGGC CGAGTCCACG 
CACAAGAGCG GCAACGTGCT GCCGGGCACC GCTGTGGACC GAGGCGTGAC GGACCCCCAC 
GCCTTCGACT TCTTCCTCAA CTCGCACGCC GGCATACAGG GCACCAACCG GGCGCCCAGG 
TACACTGTGC TTGTGGACGA GGTGGGCTTC ACGGCGGAGG CGCTGCAGCT GCTGACCCAC 
ACGCTGTGCC ACACATACCC GGCCTGCACC AGGGCGCTCT CGCTGCCGCC GCCTGTGCGC 
TACGCCGACC GGGCGGCGGA CCGCGCACGC ACACTGCGGT ACATGGTGCC GCGGCCGTCT 
GGCAAGCAGG GCCGCGGCGT GGACTGGTAC CAGATGCTGC CGACGCTGCC GCACGCAGGG 
CACGTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001694841.1
CDS	122..3013
Translation	MLSKVQLPRRAGRANSATPVSARYSDSANRPGGRGGGQPGRGGGGRGGGSGRGSSSSTAPKELLTPAQYWPSFWTRLFTGLPLGPVEPRQLANGHAARPGYSAAGKTIQVLTNHVPLNVGVRTVHQYDVRISRVANSAPTSSAAAPSGQGGGKPGRGRKEAKVSGQEAQAVLVALLGDTGAGGSGAAGGVWVFDGSQALYGVDLKLVGALDVAECLREAAQMPGPTTRRPPPDEEQPEGSPAPAATSGAAALAVLDLVTRAGVAWDVASVRTGGGSYVSYKQEDASGPVKGLLPMKLLRGFRSAVAQVAAGTTLTIDAASAVVAGDGPLLGQLQAGAEAEKTSAALAAGRDVSALATELGNSKNARRLEEALVGLEVQPSYTANRYKLSGKLGKSARSLAFEFEGRKISVQHPDLPCVLDKKGGALPIELLTVVKYQKRMSRLDARQKADYIRTAALKPADRMRAMREALTRQLGSVRGQVARAFGLAPFNDMPDFLKVPAQLLPGPLLQVAKQQAVEPGNKGEWAGGGVAVAPEWRSGAVLCYLSQSEGQAPLSGLLAALSKALSASSASALPPNIPVIWASLPKPGMSPDKAAADAEFWLNRAAAKAQEQYKRQAQMVMVVLPDRGGVGLYDAIKAAGDGKLGVATQCVNPAKAGLVGPKASSGPSFSYAAQLALSVNAKLGGATTRPAGRPNDWLPLLGGRRLMVGGRGPGRLEARLGLSGALLSRGYRGAAQVSAGVEYAAVVGSADSHAVDYRVQLSAQVAGNRDIVVSMRESVVLMYRDGLSESQFDRALAEEFTAIVQACADVGGPSYRPRICFVVVQKSHNTRFFPTSAESTHKSGNVLPGTAVDRGVTDPHAFDFFLNSHAGIQGTNRAPRYTVLVDEVGFTAEALQLLTHTLCHTYPACTRALSLPPPVRYADRAADRARTLRYMVPRPSGKQGRGVDWYQMLPTLPHAGHVV

Target ORF information:

RefSeq Version	XM_001694789.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii argonaute protein (AGO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001694789.1

ORF Insert Sequence:

ATGCTGAGCA AGGTCCAGTT GCCTCGACGG GCTGGCCGCG CGAACAGCGC AACGCCTGTG 
TCAGCTCGGT ACTCGGACTC GGCCAACCGG CCAGGAGGTC GCGGAGGCGG CCAACCGGGC 
CGGGGAGGTG GAGGCAGGGG AGGTGGCTCA GGCAGGGGCT CATCGTCGTC GACCGCGCCG 
AAGGAGCTCC TCACCCCGGC CCAGTACTGG CCGTCGTTTT GGACCCGGCT GTTCACCGGT 
CTTCCGCTGG GGCCAGTGGA GCCGCGCCAG TTGGCTAATG GACATGCGGC GCGGCCGGGT 
TACAGCGCCG CGGGCAAGAC CATCCAGGTG CTCACCAATC ACGTGCCGCT CAATGTGGGC 
GTCCGCACCG TGCATCAGTA CGACGTGCGC ATCAGCCGCG TCGCCAACAG TGCCCCGACC 
TCCTCAGCAG CAGCACCTTC GGGGCAGGGG GGCGGCAAGC CCGGCCGCGG CCGCAAGGAG 
GCCAAGGTGT CGGGGCAGGA GGCGCAGGCG GTCCTGGTGG CGCTGCTGGG CGACACAGGC 
GCAGGGGGCT CTGGAGCGGC CGGGGGCGTC TGGGTGTTCG ACGGCAGCCA GGCGCTGTAC 
GGCGTGGACC TGAAGCTGGT GGGGGCGCTG GACGTGGCAG AGTGCCTGAG GGAGGCGGCG 
CAGATGCCGG GGCCGACAAC ACGCCGGCCG CCGCCCGACG AGGAACAGCC CGAGGGCTCG 
CCCGCGCCAG CGGCCACGTC CGGCGCCGCC GCACTGGCGG TGTTGGACCT TGTGACTCGG 
GCGGGGGTTG CGTGGGACGT GGCCTCGGTG CGCACGGGCG GGGGCTCCTA CGTGTCCTAC 
AAGCAGGAGG ACGCCAGCGG GCCGGTGAAG GGCCTGCTGC CAATGAAGCT GCTGCGCGGC 
TTCCGCTCCG CTGTGGCTCA GGTGGCGGCC GGCACCACCC TCACCATTGA TGCCGCGAGT 
GCGGTGGTGG CGGGCGACGG CCCCCTGCTC GGCCAACTGC AGGCAGGCGC TGAGGCAGAG 
AAGACATCGG CTGCGCTAGC GGCGGGACGG GACGTGTCGG CGCTGGCGAC GGAGCTGGGC 
AACAGCAAGA ACGCCCGGCG CCTGGAGGAG GCGCTGGTGG GGCTGGAGGT CCAGCCCAGC 
TACACCGCCA ACCGCTACAA ACTGTCCGGC AAGTTGGGCA AGTCCGCCCG CAGCCTCGCA 
TTTGAGTTCG AGGGCCGCAA GATCAGCGTC CAGCACCCCG ACCTGCCCTG TGTGCTGGAC 
AAGAAGGGCG GCGCACTGCC CATTGAGCTG CTCACTGTGG TCAAGTACCA GAAGCGCATG 
TCGCGGCTTG ACGCCCGCCA GAAGGCCGAC TACATCCGTA CAGCCGCCCT CAAGCCGGCA 
GACCGCATGA GGGCCATGCG GGAGGCACTG ACGCGGCAGC TGGGCTCGGT GCGGGGTCAG 
GTGGCGCGCG CGTTTGGCCT GGCGCCGTTC AACGACATGC CCGACTTCCT CAAGGTGCCG 
GCGCAGCTGC TGCCTGGCCC GCTGCTGCAG GTGGCCAAGC AGCAGGCGGT GGAGCCCGGC 
AACAAGGGCG AATGGGCCGG CGGCGGCGTG GCGGTGGCGC CGGAGTGGCG CAGCGGCGCC 
GTGCTGTGCT ACCTGAGCCA GTCGGAGGGG CAGGCCCCGC TGTCGGGCCT GCTGGCCGCT 
CTGTCCAAGG CACTGTCCGC CTCGTCCGCC AGCGCCCTGC CGCCCAACAT CCCCGTCATA 
TGGGCCTCGC TGCCCAAGCC CGGCATGTCG CCCGACAAGG CCGCTGCGGA CGCGGAGTTC 
TGGCTCAACC GCGCGGCGGC CAAGGCTCAA GAGCAGTACA AGCGGCAGGC GCAGATGGTG 
ATGGTGGTGC TGCCGGACAG GGGCGGCGTA GGCCTGTATG ACGCCATCAA GGCGGCAGGC 
GACGGCAAGT TGGGAGTGGC CACCCAGTGC GTCAACCCGG CCAAGGCGGG ATTGGTGGGC 
CCCAAGGCCT CCTCCGGCCC CTCCTTCTCC TACGCGGCCC AGTTGGCGCT GTCGGTCAAC 
GCCAAACTGG GCGGCGCCAC CACGAGGCCG GCAGGCAGGC CCAATGACTG GCTGCCGCTG 
CTGGGCGGCC GGCGGCTGAT GGTGGGCGGG CGGGGGCCTG GACGGCTGGA GGCGCGGCTG 
GGGCTGAGTG GCGCACTGTT AAGTCGTGGC TATAGAGGTG CCGCCCAGGT GTCGGCTGGA 
GTCGAATACG CTGCTGTTGT GGGGTCGGCA GACAGCCACG CGGTCGACTA CCGAGTGCAG 
CTGAGTGCGC AGGTGGCGGG CAACCGCGAC ATAGTTGTCA GCATGCGCGA GAGTGTGGTG 
CTCATGTACC GGGACGGGCT GAGTGAGAGC CAGTTCGACC GGGCCCTGGC GGAGGAGTTT 
ACCGCCATTG TGCAGGCCTG CGCCGACGTG GGCGGTCCGT CCTACCGGCC GCGCATCTGC 
TTCGTGGTGG TCCAGAAGTC CCACAACACC CGCTTCTTCC CCACCTCGGC CGAGTCCACG 
CACAAGAGCG GCAACGTGCT GCCGGGCACC GCTGTGGACC GAGGCGTGAC GGACCCCCAC 
GCCTTCGACT TCTTCCTCAA CTCGCACGCC GGCATACAGG GCACCAACCG GGCGCCCAGG 
TACACTGTGC TTGTGGACGA GGTGGGCTTC ACGGCGGAGG CGCTGCAGCT GCTGACCCAC 
ACGCTGTGCC ACACATACCC GGCCTGCACC AGGGCGCTCT CGCTGCCGCC GCCTGTGCGC 
TACGCCGACC GGGCGGCGGA CCGCGCACGC ACACTGCGGT ACATGGTGCC GCGGCCGTCT 
GGCAAGCAGG GCCGCGGCGT GGACTGGTAC CAGATGCTGC CGACGCTGCC GCACGCAGGG 
CACGTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science (New York, N.Y.)318(5848)245-50(2007 Oct)
Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR

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AGO1 cDNA ORF clone, Chlamydomonas reinhardtii

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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