LOC101453056 cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101453056
Entrez Gene ID	101453056
Full Name	4-coumarate--CoA ligase 1
Synonyms	4CL2
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020857120.1	XP_020712779.1	4-coumarate--CoA ligase 1 isoform X2
XM_004520554.3	XP_004520611.1	4-coumarate--CoA ligase 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101453056 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101453056 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe177133	XM_020857120.1 Latest version!	Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe177132	XM_004520554.3 Latest version!	Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101453056 ( XM_020857120.1 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_020712779.1 Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe177133
Clone ID Related Accession (Same CDS sequence)	XM_020857120.1
Accession Version	XM_020857120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	4-coumarate--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACCT GGTCTGTACG TTTGGCTCAG CACTTCAAAA AGATGGGACT ACGTCATGAT 
GATATTATCG GCATTGTGGC CAAGAATAGT ACCTTCTTGA GCGCAGTTGT TGTCGCTTCC 
TTAATGAATT GTACACCCTT CCATGCGGTG AATCCAGTTT TCGACAAAAA TACGATTGAA 
CACATCTTTA AGACCACAGC ACCAAAGGTA ATATTCTGCG ACGGAGATTT CTATGAGAAA 
GTTCATTCAG CTACACATGC TTTAAAGCCT TTGTACTACA CACTGACCGA TCATATTGAA 
GGTGTTGCCA AAATTGAGGA CCTCTTACTG CCGTCCAAAA CCGAATTCCT CTATCAACCC 
AAACAATTGA CCTTAGGCGG TGAGCAGACT GTGGCAATAC TTTGCTCATC AGGCACCACG 
GGAATTCCAA AATGTGTTTG CATATCGAAT TACGCACTGC AAATAGAAAA TATATTTATC 
ACCAGTGAAG ATGTTATCTT CACCAATAGC TGCATTGATT GGTTTACTGG CTTGATTTTC 
ACCATTTTAG CATTCACTAT CAGCTGTAAG CGGGTGATCA CCAACCGTCC ATATACTCCC 
GAATACTTCG TTGAGCTGGT GAAAAAATAT AAAATCAATT ACGTCGGTGC TGCGCCACGT 
TACATCGCTA CACTAGTTAC CTGCCCTGAA GCCACCGCTG AGAATTTGAG CTCGATCCGC 
TCCTGTGTTA TTGGCGGTGG TATTATCAGT TTGACAACAC TTCAGCTTCT ACAAGGTATA 
CTCAAAAACG GATTGATTTC TTTTGGTTAT GGTTTGACGG AAATCGGAAC CGTTTCATTT 
AATTTTGGCC ATCAGTGTCC CACATCCGTG GGTAAATTGA TACCTAACAT GAAAGTGCGT 
ATTGTCGATG ATAATGGCAA AAATTTGCCA CACAATGAAG TTGGTGAAGT TTATGTTAAT 
ACGGGACGAT TGTGGAACGG TTATTACGGC AATCCAATTG AAACACAGCG CTTTCGAGAC 
TCACTCGGCT GGTTCCATAC CGGCGATCTC GGTTACTTTG ATGATAACAA TTTTCTCTAT 
ATTGTGGATC GTAAGAAGGA TATAATCAAA TATCAGGGCA TGCAATACTG GCCGGGTGAG 
ATTGAACAAG TTATAAGTGA ATTGCCTGAA GTGGAGGACG TTTGCGTAGT GGGTGTGTAC 
GATGAGCGCA ATGGTGATGC CGCCGGTGCT TTAGTGGTGC TTCGCAAAGG AGCCGATTTG 
ACGGAGGCAC GAGTAAAGGA CCATGTAAGA AATCGCCTAG AGGTGGTCTA TAAGCAGCTG 
CATGCGGGTG TAATTTTCGT TAACGAGTTG CCTCATAACG CCAATGGCAA GCTGCTGAAG 
AGGGAAGCAA AAGCAATGTT TGAGTCTAAA CAGTTGATTG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020712779.1
CDS	59..1486
Translation	MLTWSVRLAQHFKKMGLRHDDIIGIVAKNSTFLSAVVVASLMNCTPFHAVNPVFDKNTIEHIFKTTAPKVIFCDGDFYEKVHSATHALKPLYYTLTDHIEGVAKIEDLLLPSKTEFLYQPKQLTLGGEQTVAILCSSGTTGIPKCVCISNYALQIENIFITSEDVIFTNSCIDWFTGLIFTILAFTISCKRVITNRPYTPEYFVELVKKYKINYVGAAPRYIATLVTCPEATAENLSSIRSCVIGGGIISLTTLQLLQGILKNGLISFGYGLTEIGTVSFNFGHQCPTSVGKLIPNMKVRIVDDNGKNLPHNEVGEVYVNTGRLWNGYYGNPIETQRFRDSLGWFHTGDLGYFDDNNFLYIVDRKKDIIKYQGMQYWPGEIEQVISELPEVEDVCVVGVYDERNGDAAGALVVLRKGADLTEARVKDHVRNRLEVVYKQLHAGVIFVNELPHNANGKLLKREAKAMFESKQLIAN

Target ORF information:

RefSeq Version	XM_020857120.1
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020857120.1

ORF Insert Sequence:

ATGCTCACCT GGTCTGTACG TTTGGCTCAG CACTTCAAAA AGATGGGACT ACGTCATGAT 
GATATTATCG GCATTGTGGC CAAGAATAGT ACCTTCTTGA GCGCAGTTGT TGTCGCTTCC 
TTAATGAATT GTACACCCTT CCATGCGGTG AATCCAGTTT TCGACAAAAA TACGATTGAA 
CACATCTTTA AGACCACAGC ACCAAAGGTA ATATTCTGCG ACGGAGATTT CTATGAGAAA 
GTTCATTCAG CTACACATGC TTTAAAGCCT TTGTACTACA CACTGACCGA TCATATTGAA 
GGTGTTGCCA AAATTGAGGA CCTCTTACTG CCGTCCAAAA CCGAATTCCT CTATCAACCC 
AAACAATTGA CCTTAGGCGG TGAGCAGACT GTGGCAATAC TTTGCTCATC AGGCACCACG 
GGAATTCCAA AATGTGTTTG CATATCGAAT TACGCACTGC AAATAGAAAA TATATTTATC 
ACCAGTGAAG ATGTTATCTT CACCAATAGC TGCATTGATT GGTTTACTGG CTTGATTTTC 
ACCATTTTAG CATTCACTAT CAGCTGTAAG CGGGTGATCA CCAACCGTCC ATATACTCCC 
GAATACTTCG TTGAGCTGGT GAAAAAATAT AAAATCAATT ACGTCGGTGC TGCGCCACGT 
TACATCGCTA CACTAGTTAC CTGCCCTGAA GCCACCGCTG AGAATTTGAG CTCGATCCGC 
TCCTGTGTTA TTGGCGGTGG TATTATCAGT TTGACAACAC TTCAGCTTCT ACAAGGTATA 
CTCAAAAACG GATTGATTTC TTTTGGTTAT GGTTTGACGG AAATCGGAAC CGTTTCATTT 
AATTTTGGCC ATCAGTGTCC CACATCCGTG GGTAAATTGA TACCTAACAT GAAAGTGCGT 
ATTGTCGATG ATAATGGCAA AAATTTGCCA CACAATGAAG TTGGTGAAGT TTATGTTAAT 
ACGGGACGAT TGTGGAACGG TTATTACGGC AATCCAATTG AAACACAGCG CTTTCGAGAC 
TCACTCGGCT GGTTCCATAC CGGCGATCTC GGTTACTTTG ATGATAACAA TTTTCTCTAT 
ATTGTGGATC GTAAGAAGGA TATAATCAAA TATCAGGGCA TGCAATACTG GCCGGGTGAG 
ATTGAACAAG TTATAAGTGA ATTGCCTGAA GTGGAGGACG TTTGCGTAGT GGGTGTGTAC 
GATGAGCGCA ATGGTGATGC CGCCGGTGCT TTAGTGGTGC TTCGCAAAGG AGCCGATTTG 
ACGGAGGCAC GAGTAAAGGA CCATGTAAGA AATCGCCTAG AGGTGGTCTA TAAGCAGCTG 
CATGCGGGTG TAATTTTCGT TAACGAGTTG CCTCATAACG CCAATGGCAA GCTGCTGAAG 
AGGGAAGCAA AAGCAATGTT TGAGTCTAAA CAGTTGATTG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101453056 ( XM_004520554.3 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_004520611.1 Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe177132
Clone ID Related Accession (Same CDS sequence)	XM_004520554.3
Accession Version	XM_004520554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	4-coumarate--CoA ligase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 21, 2017 this sequence version replaced XM_004520554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCC CTGAGTGTGG TTATGATAAA TACGAGCGTG TATGGAGTGG AGGAAAGCGT 
CCATTGTTAT ACGATTTCGA TTGCTCGTTG GGCAAAGTTC TATACAACAC ATTGAAAAAT 
TATCCAAAGC ATGTTTCTCA GGTTTTCGAT CCCACCGGCC GCGAGGTTAC CAATGGCGAA 
ATGCTCACCT GGTCTGTACG TTTGGCTCAG CACTTCAAAA AGATGGGACT ACGTCATGAT 
GATATTATCG GCATTGTGGC CAAGAATAGT ACCTTCTTGA GCGCAGTTGT TGTCGCTTCC 
TTAATGAATT GTACACCCTT CCATGCGGTG AATCCAGTTT TCGACAAAAA TACGATTGAA 
CACATCTTTA AGACCACAGC ACCAAAGGTA ATATTCTGCG ACGGAGATTT CTATGAGAAA 
GTTCATTCAG CTACACATGC TTTAAAGCCT TTGTACTACA CACTGACCGA TCATATTGAA 
GGTGTTGCCA AAATTGAGGA CCTCTTACTG CCGTCCAAAA CCGAATTCCT CTATCAACCC 
AAACAATTGA CCTTAGGCGG TGAGCAGACT GTGGCAATAC TTTGCTCATC AGGCACCACG 
GGAATTCCAA AATGTGTTTG CATATCGAAT TACGCACTGC AAATAGAAAA TATATTTATC 
ACCAGTGAAG ATGTTATCTT CACCAATAGC TGCATTGATT GGTTTACTGG CTTGATTTTC 
ACCATTTTAG CATTCACTAT CAGCTGTAAG CGGGTGATCA CCAACCGTCC ATATACTCCC 
GAATACTTCG TTGAGCTGGT GAAAAAATAT AAAATCAATT ACGTCGGTGC TGCGCCACGT 
TACATCGCTA CACTAGTTAC CTGCCCTGAA GCCACCGCTG AGAATTTGAG CTCGATCCGC 
TCCTGTGTTA TTGGCGGTGG TATTATCAGT TTGACAACAC TTCAGCTTCT ACAAGGTATA 
CTCAAAAACG GATTGATTTC TTTTGGTTAT GGTTTGACGG AAATCGGAAC CGTTTCATTT 
AATTTTGGCC ATCAGTGTCC CACATCCGTG GGTAAATTGA TACCTAACAT GAAAGTGCGT 
ATTGTCGATG ATAATGGCAA AAATTTGCCA CACAATGAAG TTGGTGAAGT TTATGTTAAT 
ACGGGACGAT TGTGGAACGG TTATTACGGC AATCCAATTG AAACACAGCG CTTTCGAGAC 
TCACTCGGCT GGTTCCATAC CGGCGATCTC GGTTACTTTG ATGATAACAA TTTTCTCTAT 
ATTGTGGATC GTAAGAAGGA TATAATCAAA TATCAGGGCA TGCAATACTG GCCGGGTGAG 
ATTGAACAAG TTATAAGTGA ATTGCCTGAA GTGGAGGACG TTTGCGTAGT GGGTGTGTAC 
GATGAGCGCA ATGGTGATGC CGCCGGTGCT TTAGTGGTGC TTCGCAAAGG AGCCGATTTG 
ACGGAGGCAC GAGTAAAGGA CCATGTAAGA AATCGCCTAG AGGTGGTCTA TAAGCAGCTG 
CATGCGGGTG TAATTTTCGT TAACGAGTTG CCTCATAACG CCAATGGCAA GCTGCTGAAG 
AGGGAAGCAA AAGCAATGTT TGAGTCTAAA CAGTTGATTG CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004520611.1
CDS	62..1669
Translation	MDFPECGYDKYERVWSGGKRPLLYDFDCSLGKVLYNTLKNYPKHVSQVFDPTGREVTNGEMLTWSVRLAQHFKKMGLRHDDIIGIVAKNSTFLSAVVVASLMNCTPFHAVNPVFDKNTIEHIFKTTAPKVIFCDGDFYEKVHSATHALKPLYYTLTDHIEGVAKIEDLLLPSKTEFLYQPKQLTLGGEQTVAILCSSGTTGIPKCVCISNYALQIENIFITSEDVIFTNSCIDWFTGLIFTILAFTISCKRVITNRPYTPEYFVELVKKYKINYVGAAPRYIATLVTCPEATAENLSSIRSCVIGGGIISLTTLQLLQGILKNGLISFGYGLTEIGTVSFNFGHQCPTSVGKLIPNMKVRIVDDNGKNLPHNEVGEVYVNTGRLWNGYYGNPIETQRFRDSLGWFHTGDLGYFDDNNFLYIVDRKKDIIKYQGMQYWPGEIEQVISELPEVEDVCVVGVYDERNGDAAGALVVLRKGADLTEARVKDHVRNRLEVVYKQLHAGVIFVNELPHNANGKLLKREAKAMFESKQLIAN

Target ORF information:

RefSeq Version	XM_004520554.3
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata 4-coumarate--CoA ligase 1 (LOC101453056), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004520554.3

ORF Insert Sequence:

ATGGATTTCC CTGAGTGTGG TTATGATAAA TACGAGCGTG TATGGAGTGG AGGAAAGCGT 
CCATTGTTAT ACGATTTCGA TTGCTCGTTG GGCAAAGTTC TATACAACAC ATTGAAAAAT 
TATCCAAAGC ATGTTTCTCA GGTTTTCGAT CCCACCGGCC GCGAGGTTAC CAATGGCGAA 
ATGCTCACCT GGTCTGTACG TTTGGCTCAG CACTTCAAAA AGATGGGACT ACGTCATGAT 
GATATTATCG GCATTGTGGC CAAGAATAGT ACCTTCTTGA GCGCAGTTGT TGTCGCTTCC 
TTAATGAATT GTACACCCTT CCATGCGGTG AATCCAGTTT TCGACAAAAA TACGATTGAA 
CACATCTTTA AGACCACAGC ACCAAAGGTA ATATTCTGCG ACGGAGATTT CTATGAGAAA 
GTTCATTCAG CTACACATGC TTTAAAGCCT TTGTACTACA CACTGACCGA TCATATTGAA 
GGTGTTGCCA AAATTGAGGA CCTCTTACTG CCGTCCAAAA CCGAATTCCT CTATCAACCC 
AAACAATTGA CCTTAGGCGG TGAGCAGACT GTGGCAATAC TTTGCTCATC AGGCACCACG 
GGAATTCCAA AATGTGTTTG CATATCGAAT TACGCACTGC AAATAGAAAA TATATTTATC 
ACCAGTGAAG ATGTTATCTT CACCAATAGC TGCATTGATT GGTTTACTGG CTTGATTTTC 
ACCATTTTAG CATTCACTAT CAGCTGTAAG CGGGTGATCA CCAACCGTCC ATATACTCCC 
GAATACTTCG TTGAGCTGGT GAAAAAATAT AAAATCAATT ACGTCGGTGC TGCGCCACGT 
TACATCGCTA CACTAGTTAC CTGCCCTGAA GCCACCGCTG AGAATTTGAG CTCGATCCGC 
TCCTGTGTTA TTGGCGGTGG TATTATCAGT TTGACAACAC TTCAGCTTCT ACAAGGTATA 
CTCAAAAACG GATTGATTTC TTTTGGTTAT GGTTTGACGG AAATCGGAAC CGTTTCATTT 
AATTTTGGCC ATCAGTGTCC CACATCCGTG GGTAAATTGA TACCTAACAT GAAAGTGCGT 
ATTGTCGATG ATAATGGCAA AAATTTGCCA CACAATGAAG TTGGTGAAGT TTATGTTAAT 
ACGGGACGAT TGTGGAACGG TTATTACGGC AATCCAATTG AAACACAGCG CTTTCGAGAC 
TCACTCGGCT GGTTCCATAC CGGCGATCTC GGTTACTTTG ATGATAACAA TTTTCTCTAT 
ATTGTGGATC GTAAGAAGGA TATAATCAAA TATCAGGGCA TGCAATACTG GCCGGGTGAG 
ATTGAACAAG TTATAAGTGA ATTGCCTGAA GTGGAGGACG TTTGCGTAGT GGGTGTGTAC 
GATGAGCGCA ATGGTGATGC CGCCGGTGCT TTAGTGGTGC TTCGCAAAGG AGCCGATTTG 
ACGGAGGCAC GAGTAAAGGA CCATGTAAGA AATCGCCTAG AGGTGGTCTA TAAGCAGCTG 
CATGCGGGTG TAATTTTCGT TAACGAGTTG CCTCATAACG CCAATGGCAA GCTGCTGAAG 
AGGGAAGCAA AAGCAATGTT TGAGTCTAAA CAGTTGATTG CAAATTAA