LOC107270021 cDNA ORF clone, Cephus cinctus(wheat stem sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107270021
Entrez Gene ID	107270021
Full Name	E3 ubiquitin-protein ligase MIB2
Gene Type	protein-coding
Organism	Cephus cinctus(wheat stem sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015744622.2	XP_015600108.1	E3 ubiquitin-protein ligase MIB2
XM_015744622.1	XP_015600108.1	E3 ubiquitin-protein ligase MIB2

Pathways from BioSystems
Gene Ontology

The following LOC107270021 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107270021 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe89796	XM_015744622.2 Latest version!	Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OCe89796	XM_015744622.1	Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107270021 ( XM_015744622.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015600108.1 Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.

Price & Availability↑

CloneID	OCe89796
Clone ID Related Accession (Same CDS sequence)	XM_015744622.2 , XM_015744622.1
Accession Version	XM_015744622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	E3 ubiquitin-protein ligase MIB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014333318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015744622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGAAG TTGGCTTGAG GGTTGTACGT GGCCAGGATT GGAAATGGGG AGATCAGGAT 
GGGGGAGAAG GCCATGCAGG GACTGTGGTC GAAATAGGAA AGCCACCCGT TCCTGGGAAT 
TCCGGTTCTT CATCTAATTC TACCGATAAG ACTCCAGATA AAACAGTCAT AGTTCAATGG 
GATCATGGAT CGAGAAGCAA CTACAGAGTT GGATATCAGG GAGCTTATGA TATTCTTGTT 
TTTGACAATG CTGCTGCAGG AGTGAAGCAC TCAAGTATTA TTTGTGACGG ATGTAAAAAA 
CATGGAATTG TCGGTATTAG ATGGAAGTGT GCACAATGCT TTGATTATGA TCTGTGCACA 
CAGTGTTACA TGTCAGATAT TCATGATTTG AGTCATACAT TTCAAAGATT TCAAACAACG 
AATTCAACTG GAGTGCAATT GAGTACAAGA GACGGTTGTA CCAAAATACC ATTGAAAGGA 
ATTTTTATTG GAGCAAAGGT AGTACGAGGT CCTGATTGGG AATGGGGTGT ACAAGATGGC 
GGACGTGGAA AAACTGGCAG AGTAATGGAC ATTCGAGGTT GGGACAATGA GAGTGGTCGT 
TCCGTAGCCA CTGTAACCTG GTCCACTGGT AGTACCAATG TTTACAGACT TGGCTACAAG 
GGCTGTGTGG ATCTCCGTTA CATAGAAGAA GCCACTTGTG GCACCTACTA CAAGGAACAC 
TTGCCTCTAT TAGGACAACC AGTAATGACA ATGTCAGAAG GTGATCAAAG CACAACTCCC 
AATGGTGGTG GTGTTGGTAG CAGTGCACCA AGTCCACGTC ATTTGACGTT TAACGTTGGG 
GATAAGGTGA AAGTCCTGAA GGAAGTTGAA ATGTTGAAGG AGATGCAAGC TGGTCACGGT 
GGATGGAACC CACGCATGGC TGAGTACATC GGAAAGATTG GCACTGTACA TCGTGTCACG 
GACAAGGGTG ACATCCGAGT ACAATACGAG GGCTGCAACA ACAGGTGGAC CTTCCACCCT 
GGAGCATTGT CCAAGGTTAT TACCAAAGAC TCATTTTCTC TTGGCGACAT AGTCAGAGTA 
AAGACCGATG CAGCGGTTGT TAAACATTGT CAAAGAGGTC ATGGGGAATG GATCGATGTC 
ATGAGAACTG CTCTTGGGAA AACGGGTAAA GTCATTAAAA TATATCCTGA CGGTGATCTA 
CTTGTGGCAC TGGAGGGTCA TACCTGGACA TTTAATCCTT TGAGCGTAAA CTTAGTACCT 
CCGAGCACAG ATCCAGCCAA CATGATGCAG GGTGAAGCCA ATAGACGTAG GGATCGATCA 
GTTGGCTCGA CACTTAGTGA GGGTGCGGAT ATCGAGGTGG AGAAGCTTCT GCGTGATGCA 
GCACGTGGTG AAGCTGGTGT TGCTGCCGTA CGCGAATTCC TCCGAAAATA TCCAGGCAAA 
GTGGATGCTC GAGCAGGAGG TCCTGGTGGT GGTAAAAAAA CCTGTCTCCA AGTAGCCGCT 
CACCAGGGTC AACGTGAACT TTGTAATCTT CTTCTGGATG CTGGAGCTTC CCTTCGTGCT 
GTTGACGAAG ATGGTGACAC TCCGTTGCAC TATGCTGCTT TTGGAAACCA ACCGGAGATA 
ATGGAACTTC TTCTTTCTCG CGGTGCAGCA ATAGATGCTG TAAACAATGG TCGTTGCAGT 
GCGTTACACG TGGCTGTGAA TAAGCAGCAC GCTGCTTGCG TGAGAGCCTT GCTACGTCAT 
GGATGTGATG TAAACTTACA GGACTCTTAC GGCGACACAG CACTGCACGA TGCGATTGGT 
AAGGATGCCC TGGAGATAGT CGATGCACTT TGTTCCTGCG AGAGGACCAA CTTTACACTG 
CGCAACAAGC GTGGTTTTAA TGTTCTGCAT CACGCTGCTC TTAAGGGAAA CGCTCACGCA 
ACGGAGCGAC TGGTAGCCAG AGCCAGACAG CTCGTGGACG TAAAGAAAGA GGACGGATTC 
GCTGCACTTC ATCTTGCTAC ATTGAACGGG CATCGCGAAG TAGCAGCTAT ATTACTTTCC 
CAAAATGGTG GTCGTGCCAC GATTGATCTG TGGAATAAAC GAAGGCAGAC ACCCTTGCAT 
TTAGCCACAG CTCAGGGTCA TTGGGCCCTG GTCGAACTTC TCATCAATCA CAATGCGGAT 
CTCGCGGCTA CCGATGAGGA CGGAGATACC GTTCTGCATC TTGCTATTTC GAAATGCAGA 
CACCAGCCAG CTACTGTAGC AACTCCAGAA AGTAGTCGAG ACTCGCCCTT TATTCATGCG 
ATCTGGCAGG ACCTTGCGAG ACAAGGTGCA AAGACTGAAA TGGCTCTTGC TTGTTTTCTT 
GTCAGTGTCG ATACCACTGA ACTCCTATTA GATCAAACTA GAAACTCGAA AGGGAAGACG 
GCTCTAGATC TTTTAGAGAC GGATCCAGAC GCTCCTGCAC TGACTGCTCT TTTACTCTCT 
TATCAACCAC ACAGACGCAA TAGCAACGAC TTGGTCCCGT CCGAGACTGT TTCATCCGGC 
GGCAGTGTAG AGACACCGAC ATACGAGATT GAAACTATAT CAGAACCGGA AGTCACGAAT 
GAAGAATCGA GAAAGCCTCC TAGGAGTTCC GGTGGCTCTG ACCAGTGCCG TGGATGCTCT 
GCGTTACTGA CTCAGGGTGT AGAAGAAACT AGAATAACAG CCGGAGGCAG TGAGACATTC 
GTAGGCTGCA TACACTGTGG TCACACGGTA CCAAAATCTC AGATTAATCG AGATGGCCAT 
GGATTCAAAG ATGACATGGC AGAGGGCGAA GACCGAAAAA GGGAGGGTCT AGACGATAGA 
AGAAATGAAG ACAAAGCAAA GGACAAGGAT ATAGAAAAGG AAAAAGATCT GGAACGGCTA 
CGCTACTTAG AGACAAGGGT AGCCGATCTC GAGGAAGCGA ACATGTGCAG CATCTGCATG 
GAGCGTCGTA GAAACGTCGC TTTCCTCTGT GGTCACGGCG CATGCGAACA CTGTGCCGCG 
CCCTTGAAAA CCTGCCACAT GTGTCGCAAG ACTATAACCA AAAAGATCAA TCTCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015600108.1
CDS	173..3232
Translation	MLEVGLRVVRGQDWKWGDQDGGEGHAGTVVEIGKPPVPGNSGSSSNSTDKTPDKTVIVQWDHGSRSNYRVGYQGAYDILVFDNAAAGVKHSSIICDGCKKHGIVGIRWKCAQCFDYDLCTQCYMSDIHDLSHTFQRFQTTNSTGVQLSTRDGCTKIPLKGIFIGAKVVRGPDWEWGVQDGGRGKTGRVMDIRGWDNESGRSVATVTWSTGSTNVYRLGYKGCVDLRYIEEATCGTYYKEHLPLLGQPVMTMSEGDQSTTPNGGGVGSSAPSPRHLTFNVGDKVKVLKEVEMLKEMQAGHGGWNPRMAEYIGKIGTVHRVTDKGDIRVQYEGCNNRWTFHPGALSKVITKDSFSLGDIVRVKTDAAVVKHCQRGHGEWIDVMRTALGKTGKVIKIYPDGDLLVALEGHTWTFNPLSVNLVPPSTDPANMMQGEANRRRDRSVGSTLSEGADIEVEKLLRDAARGEAGVAAVREFLRKYPGKVDARAGGPGGGKKTCLQVAAHQGQRELCNLLLDAGASLRAVDEDGDTPLHYAAFGNQPEIMELLLSRGAAIDAVNNGRCSALHVAVNKQHAACVRALLRHGCDVNLQDSYGDTALHDAIGKDALEIVDALCSCERTNFTLRNKRGFNVLHHAALKGNAHATERLVARARQLVDVKKEDGFAALHLATLNGHREVAAILLSQNGGRATIDLWNKRRQTPLHLATAQGHWALVELLINHNADLAATDEDGDTVLHLAISKCRHQPATVATPESSRDSPFIHAIWQDLARQGAKTEMALACFLVSVDTTELLLDQTRNSKGKTALDLLETDPDAPALTALLLSYQPHRRNSNDLVPSETVSSGGSVETPTYEIETISEPEVTNEESRKPPRSSGGSDQCRGCSALLTQGVEETRITAGGSETFVGCIHCGHTVPKSQINRDGHGFKDDMAEGEDRKREGLDDRRNEDKAKDKDIEKEKDLERLRYLETRVADLEEANMCSICMERRRNVAFLCGHGACEHCAAPLKTCHMCRKTITKKINLY

Target ORF information:

RefSeq Version	XM_015744622.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015744622.2

ORF Insert Sequence:

ATGCTTGAAG TTGGCTTGAG GGTTGTACGT GGCCAGGATT GGAAATGGGG AGATCAGGAT 
GGGGGAGAAG GCCATGCAGG GACTGTGGTC GAAATAGGAA AGCCACCCGT TCCTGGGAAT 
TCCGGTTCTT CATCTAATTC TACCGATAAG ACTCCAGATA AAACAGTCAT AGTTCAATGG 
GATCATGGAT CGAGAAGCAA CTACAGAGTT GGATATCAGG GAGCTTATGA TATTCTTGTT 
TTTGACAATG CTGCTGCAGG AGTGAAGCAC TCAAGTATTA TTTGTGACGG ATGTAAAAAA 
CATGGAATTG TCGGTATTAG ATGGAAGTGT GCACAATGCT TTGATTATGA TCTGTGCACA 
CAGTGTTACA TGTCAGATAT TCATGATTTG AGTCATACAT TTCAAAGATT TCAAACAACG 
AATTCAACTG GAGTGCAATT GAGTACAAGA GACGGTTGTA CCAAAATACC ATTGAAAGGA 
ATTTTTATTG GAGCAAAGGT AGTACGAGGT CCTGATTGGG AATGGGGTGT ACAAGATGGC 
GGACGTGGAA AAACTGGCAG AGTAATGGAC ATTCGAGGTT GGGACAATGA GAGTGGTCGT 
TCCGTAGCCA CTGTAACCTG GTCCACTGGT AGTACCAATG TTTACAGACT TGGCTACAAG 
GGCTGTGTGG ATCTCCGTTA CATAGAAGAA GCCACTTGTG GCACCTACTA CAAGGAACAC 
TTGCCTCTAT TAGGACAACC AGTAATGACA ATGTCAGAAG GTGATCAAAG CACAACTCCC 
AATGGTGGTG GTGTTGGTAG CAGTGCACCA AGTCCACGTC ATTTGACGTT TAACGTTGGG 
GATAAGGTGA AAGTCCTGAA GGAAGTTGAA ATGTTGAAGG AGATGCAAGC TGGTCACGGT 
GGATGGAACC CACGCATGGC TGAGTACATC GGAAAGATTG GCACTGTACA TCGTGTCACG 
GACAAGGGTG ACATCCGAGT ACAATACGAG GGCTGCAACA ACAGGTGGAC CTTCCACCCT 
GGAGCATTGT CCAAGGTTAT TACCAAAGAC TCATTTTCTC TTGGCGACAT AGTCAGAGTA 
AAGACCGATG CAGCGGTTGT TAAACATTGT CAAAGAGGTC ATGGGGAATG GATCGATGTC 
ATGAGAACTG CTCTTGGGAA AACGGGTAAA GTCATTAAAA TATATCCTGA CGGTGATCTA 
CTTGTGGCAC TGGAGGGTCA TACCTGGACA TTTAATCCTT TGAGCGTAAA CTTAGTACCT 
CCGAGCACAG ATCCAGCCAA CATGATGCAG GGTGAAGCCA ATAGACGTAG GGATCGATCA 
GTTGGCTCGA CACTTAGTGA GGGTGCGGAT ATCGAGGTGG AGAAGCTTCT GCGTGATGCA 
GCACGTGGTG AAGCTGGTGT TGCTGCCGTA CGCGAATTCC TCCGAAAATA TCCAGGCAAA 
GTGGATGCTC GAGCAGGAGG TCCTGGTGGT GGTAAAAAAA CCTGTCTCCA AGTAGCCGCT 
CACCAGGGTC AACGTGAACT TTGTAATCTT CTTCTGGATG CTGGAGCTTC CCTTCGTGCT 
GTTGACGAAG ATGGTGACAC TCCGTTGCAC TATGCTGCTT TTGGAAACCA ACCGGAGATA 
ATGGAACTTC TTCTTTCTCG CGGTGCAGCA ATAGATGCTG TAAACAATGG TCGTTGCAGT 
GCGTTACACG TGGCTGTGAA TAAGCAGCAC GCTGCTTGCG TGAGAGCCTT GCTACGTCAT 
GGATGTGATG TAAACTTACA GGACTCTTAC GGCGACACAG CACTGCACGA TGCGATTGGT 
AAGGATGCCC TGGAGATAGT CGATGCACTT TGTTCCTGCG AGAGGACCAA CTTTACACTG 
CGCAACAAGC GTGGTTTTAA TGTTCTGCAT CACGCTGCTC TTAAGGGAAA CGCTCACGCA 
ACGGAGCGAC TGGTAGCCAG AGCCAGACAG CTCGTGGACG TAAAGAAAGA GGACGGATTC 
GCTGCACTTC ATCTTGCTAC ATTGAACGGG CATCGCGAAG TAGCAGCTAT ATTACTTTCC 
CAAAATGGTG GTCGTGCCAC GATTGATCTG TGGAATAAAC GAAGGCAGAC ACCCTTGCAT 
TTAGCCACAG CTCAGGGTCA TTGGGCCCTG GTCGAACTTC TCATCAATCA CAATGCGGAT 
CTCGCGGCTA CCGATGAGGA CGGAGATACC GTTCTGCATC TTGCTATTTC GAAATGCAGA 
CACCAGCCAG CTACTGTAGC AACTCCAGAA AGTAGTCGAG ACTCGCCCTT TATTCATGCG 
ATCTGGCAGG ACCTTGCGAG ACAAGGTGCA AAGACTGAAA TGGCTCTTGC TTGTTTTCTT 
GTCAGTGTCG ATACCACTGA ACTCCTATTA GATCAAACTA GAAACTCGAA AGGGAAGACG 
GCTCTAGATC TTTTAGAGAC GGATCCAGAC GCTCCTGCAC TGACTGCTCT TTTACTCTCT 
TATCAACCAC ACAGACGCAA TAGCAACGAC TTGGTCCCGT CCGAGACTGT TTCATCCGGC 
GGCAGTGTAG AGACACCGAC ATACGAGATT GAAACTATAT CAGAACCGGA AGTCACGAAT 
GAAGAATCGA GAAAGCCTCC TAGGAGTTCC GGTGGCTCTG ACCAGTGCCG TGGATGCTCT 
GCGTTACTGA CTCAGGGTGT AGAAGAAACT AGAATAACAG CCGGAGGCAG TGAGACATTC 
GTAGGCTGCA TACACTGTGG TCACACGGTA CCAAAATCTC AGATTAATCG AGATGGCCAT 
GGATTCAAAG ATGACATGGC AGAGGGCGAA GACCGAAAAA GGGAGGGTCT AGACGATAGA 
AGAAATGAAG ACAAAGCAAA GGACAAGGAT ATAGAAAAGG AAAAAGATCT GGAACGGCTA 
CGCTACTTAG AGACAAGGGT AGCCGATCTC GAGGAAGCGA ACATGTGCAG CATCTGCATG 
GAGCGTCGTA GAAACGTCGC TTTCCTCTGT GGTCACGGCG CATGCGAACA CTGTGCCGCG 
CCCTTGAAAA CCTGCCACAT GTGTCGCAAG ACTATAACCA AAAAGATCAA TCTCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107270021 ( XM_015744622.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015600108.1 Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.

Price & Availability↑

CloneID	OCe89796
Clone ID Related Accession (Same CDS sequence)	XM_015744622.2 , XM_015744622.1
Accession Version	XM_015744622.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	E3 ubiquitin-protein ligase MIB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014333318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGAAG TTGGCTTGAG GGTTGTACGT GGCCAGGATT GGAAATGGGG AGATCAGGAT 
GGGGGAGAAG GCCATGCAGG GACTGTGGTC GAAATAGGAA AGCCACCCGT TCCTGGGAAT 
TCCGGTTCTT CATCTAATTC TACCGATAAG ACTCCAGATA AAACAGTCAT AGTTCAATGG 
GATCATGGAT CGAGAAGCAA CTACAGAGTT GGATATCAGG GAGCTTATGA TATTCTTGTT 
TTTGACAATG CTGCTGCAGG AGTGAAGCAC TCAAGTATTA TTTGTGACGG ATGTAAAAAA 
CATGGAATTG TCGGTATTAG ATGGAAGTGT GCACAATGCT TTGATTATGA TCTGTGCACA 
CAGTGTTACA TGTCAGATAT TCATGATTTG AGTCATACAT TTCAAAGATT TCAAACAACG 
AATTCAACTG GAGTGCAATT GAGTACAAGA GACGGTTGTA CCAAAATACC ATTGAAAGGA 
ATTTTTATTG GAGCAAAGGT AGTACGAGGT CCTGATTGGG AATGGGGTGT ACAAGATGGC 
GGACGTGGAA AAACTGGCAG AGTAATGGAC ATTCGAGGTT GGGACAATGA GAGTGGTCGT 
TCCGTAGCCA CTGTAACCTG GTCCACTGGT AGTACCAATG TTTACAGACT TGGCTACAAG 
GGCTGTGTGG ATCTCCGTTA CATAGAAGAA GCCACTTGTG GCACCTACTA CAAGGAACAC 
TTGCCTCTAT TAGGACAACC AGTAATGACA ATGTCAGAAG GTGATCAAAG CACAACTCCC 
AATGGTGGTG GTGTTGGTAG CAGTGCACCA AGTCCACGTC ATTTGACGTT TAACGTTGGG 
GATAAGGTGA AAGTCCTGAA GGAAGTTGAA ATGTTGAAGG AGATGCAAGC TGGTCACGGT 
GGATGGAACC CACGCATGGC TGAGTACATC GGAAAGATTG GCACTGTACA TCGTGTCACG 
GACAAGGGTG ACATCCGAGT ACAATACGAG GGCTGCAACA ACAGGTGGAC CTTCCACCCT 
GGAGCATTGT CCAAGGTTAT TACCAAAGAC TCATTTTCTC TTGGCGACAT AGTCAGAGTA 
AAGACCGATG CAGCGGTTGT TAAACATTGT CAAAGAGGTC ATGGGGAATG GATCGATGTC 
ATGAGAACTG CTCTTGGGAA AACGGGTAAA GTCATTAAAA TATATCCTGA CGGTGATCTA 
CTTGTGGCAC TGGAGGGTCA TACCTGGACA TTTAATCCTT TGAGCGTAAA CTTAGTACCT 
CCGAGCACAG ATCCAGCCAA CATGATGCAG GGTGAAGCCA ATAGACGTAG GGATCGATCA 
GTTGGCTCGA CACTTAGTGA GGGTGCGGAT ATCGAGGTGG AGAAGCTTCT GCGTGATGCA 
GCACGTGGTG AAGCTGGTGT TGCTGCCGTA CGCGAATTCC TCCGAAAATA TCCAGGCAAA 
GTGGATGCTC GAGCAGGAGG TCCTGGTGGT GGTAAAAAAA CCTGTCTCCA AGTAGCCGCT 
CACCAGGGTC AACGTGAACT TTGTAATCTT CTTCTGGATG CTGGAGCTTC CCTTCGTGCT 
GTTGACGAAG ATGGTGACAC TCCGTTGCAC TATGCTGCTT TTGGAAACCA ACCGGAGATA 
ATGGAACTTC TTCTTTCTCG CGGTGCAGCA ATAGATGCTG TAAACAATGG TCGTTGCAGT 
GCGTTACACG TGGCTGTGAA TAAGCAGCAC GCTGCTTGCG TGAGAGCCTT GCTACGTCAT 
GGATGTGATG TAAACTTACA GGACTCTTAC GGCGACACAG CACTGCACGA TGCGATTGGT 
AAGGATGCCC TGGAGATAGT CGATGCACTT TGTTCCTGCG AGAGGACCAA CTTTACACTG 
CGCAACAAGC GTGGTTTTAA TGTTCTGCAT CACGCTGCTC TTAAGGGAAA CGCTCACGCA 
ACGGAGCGAC TGGTAGCCAG AGCCAGACAG CTCGTGGACG TAAAGAAAGA GGACGGATTC 
GCTGCACTTC ATCTTGCTAC ATTGAACGGG CATCGCGAAG TAGCAGCTAT ATTACTTTCC 
CAAAATGGTG GTCGTGCCAC GATTGATCTG TGGAATAAAC GAAGGCAGAC ACCCTTGCAT 
TTAGCCACAG CTCAGGGTCA TTGGGCCCTG GTCGAACTTC TCATCAATCA CAATGCGGAT 
CTCGCGGCTA CCGATGAGGA CGGAGATACC GTTCTGCATC TTGCTATTTC GAAATGCAGA 
CACCAGCCAG CTACTGTAGC AACTCCAGAA AGTAGTCGAG ACTCGCCCTT TATTCATGCG 
ATCTGGCAGG ACCTTGCGAG ACAAGGTGCA AAGACTGAAA TGGCTCTTGC TTGTTTTCTT 
GTCAGTGTCG ATACCACTGA ACTCCTATTA GATCAAACTA GAAACTCGAA AGGGAAGACG 
GCTCTAGATC TTTTAGAGAC GGATCCAGAC GCTCCTGCAC TGACTGCTCT TTTACTCTCT 
TATCAACCAC ACAGACGCAA TAGCAACGAC TTGGTCCCGT CCGAGACTGT TTCATCCGGC 
GGCAGTGTAG AGACACCGAC ATACGAGATT GAAACTATAT CAGAACCGGA AGTCACGAAT 
GAAGAATCGA GAAAGCCTCC TAGGAGTTCC GGTGGCTCTG ACCAGTGCCG TGGATGCTCT 
GCGTTACTGA CTCAGGGTGT AGAAGAAACT AGAATAACAG CCGGAGGCAG TGAGACATTC 
GTAGGCTGCA TACACTGTGG TCACACGGTA CCAAAATCTC AGATTAATCG AGATGGCCAT 
GGATTCAAAG ATGACATGGC AGAGGGCGAA GACCGAAAAA GGGAGGGTCT AGACGATAGA 
AGAAATGAAG ACAAAGCAAA GGACAAGGAT ATAGAAAAGG AAAAAGATCT GGAACGGCTA 
CGCTACTTAG AGACAAGGGT AGCCGATCTC GAGGAAGCGA ACATGTGCAG CATCTGCATG 
GAGCGTCGTA GAAACGTCGC TTTCCTCTGT GGTCACGGCG CATGCGAACA CTGTGCCGCG 
CCCTTGAAAA CCTGCCACAT GTGTCGCAAG ACTATAACCA AAAAGATCAA TCTCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015600108.1
CDS	172..3231
Translation	MLEVGLRVVRGQDWKWGDQDGGEGHAGTVVEIGKPPVPGNSGSSSNSTDKTPDKTVIVQWDHGSRSNYRVGYQGAYDILVFDNAAAGVKHSSIICDGCKKHGIVGIRWKCAQCFDYDLCTQCYMSDIHDLSHTFQRFQTTNSTGVQLSTRDGCTKIPLKGIFIGAKVVRGPDWEWGVQDGGRGKTGRVMDIRGWDNESGRSVATVTWSTGSTNVYRLGYKGCVDLRYIEEATCGTYYKEHLPLLGQPVMTMSEGDQSTTPNGGGVGSSAPSPRHLTFNVGDKVKVLKEVEMLKEMQAGHGGWNPRMAEYIGKIGTVHRVTDKGDIRVQYEGCNNRWTFHPGALSKVITKDSFSLGDIVRVKTDAAVVKHCQRGHGEWIDVMRTALGKTGKVIKIYPDGDLLVALEGHTWTFNPLSVNLVPPSTDPANMMQGEANRRRDRSVGSTLSEGADIEVEKLLRDAARGEAGVAAVREFLRKYPGKVDARAGGPGGGKKTCLQVAAHQGQRELCNLLLDAGASLRAVDEDGDTPLHYAAFGNQPEIMELLLSRGAAIDAVNNGRCSALHVAVNKQHAACVRALLRHGCDVNLQDSYGDTALHDAIGKDALEIVDALCSCERTNFTLRNKRGFNVLHHAALKGNAHATERLVARARQLVDVKKEDGFAALHLATLNGHREVAAILLSQNGGRATIDLWNKRRQTPLHLATAQGHWALVELLINHNADLAATDEDGDTVLHLAISKCRHQPATVATPESSRDSPFIHAIWQDLARQGAKTEMALACFLVSVDTTELLLDQTRNSKGKTALDLLETDPDAPALTALLLSYQPHRRNSNDLVPSETVSSGGSVETPTYEIETISEPEVTNEESRKPPRSSGGSDQCRGCSALLTQGVEETRITAGGSETFVGCIHCGHTVPKSQINRDGHGFKDDMAEGEDRKREGLDDRRNEDKAKDKDIEKEKDLERLRYLETRVADLEEANMCSICMERRRNVAFLCGHGACEHCAAPLKTCHMCRKTITKKINLY

Target ORF information:

RefSeq Version	XM_015744622.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus E3 ubiquitin-protein ligase MIB2 (LOC107270021), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015744622.1

ORF Insert Sequence:

ATGCTTGAAG TTGGCTTGAG GGTTGTACGT GGCCAGGATT GGAAATGGGG AGATCAGGAT 
GGGGGAGAAG GCCATGCAGG GACTGTGGTC GAAATAGGAA AGCCACCCGT TCCTGGGAAT 
TCCGGTTCTT CATCTAATTC TACCGATAAG ACTCCAGATA AAACAGTCAT AGTTCAATGG 
GATCATGGAT CGAGAAGCAA CTACAGAGTT GGATATCAGG GAGCTTATGA TATTCTTGTT 
TTTGACAATG CTGCTGCAGG AGTGAAGCAC TCAAGTATTA TTTGTGACGG ATGTAAAAAA 
CATGGAATTG TCGGTATTAG ATGGAAGTGT GCACAATGCT TTGATTATGA TCTGTGCACA 
CAGTGTTACA TGTCAGATAT TCATGATTTG AGTCATACAT TTCAAAGATT TCAAACAACG 
AATTCAACTG GAGTGCAATT GAGTACAAGA GACGGTTGTA CCAAAATACC ATTGAAAGGA 
ATTTTTATTG GAGCAAAGGT AGTACGAGGT CCTGATTGGG AATGGGGTGT ACAAGATGGC 
GGACGTGGAA AAACTGGCAG AGTAATGGAC ATTCGAGGTT GGGACAATGA GAGTGGTCGT 
TCCGTAGCCA CTGTAACCTG GTCCACTGGT AGTACCAATG TTTACAGACT TGGCTACAAG 
GGCTGTGTGG ATCTCCGTTA CATAGAAGAA GCCACTTGTG GCACCTACTA CAAGGAACAC 
TTGCCTCTAT TAGGACAACC AGTAATGACA ATGTCAGAAG GTGATCAAAG CACAACTCCC 
AATGGTGGTG GTGTTGGTAG CAGTGCACCA AGTCCACGTC ATTTGACGTT TAACGTTGGG 
GATAAGGTGA AAGTCCTGAA GGAAGTTGAA ATGTTGAAGG AGATGCAAGC TGGTCACGGT 
GGATGGAACC CACGCATGGC TGAGTACATC GGAAAGATTG GCACTGTACA TCGTGTCACG 
GACAAGGGTG ACATCCGAGT ACAATACGAG GGCTGCAACA ACAGGTGGAC CTTCCACCCT 
GGAGCATTGT CCAAGGTTAT TACCAAAGAC TCATTTTCTC TTGGCGACAT AGTCAGAGTA 
AAGACCGATG CAGCGGTTGT TAAACATTGT CAAAGAGGTC ATGGGGAATG GATCGATGTC 
ATGAGAACTG CTCTTGGGAA AACGGGTAAA GTCATTAAAA TATATCCTGA CGGTGATCTA 
CTTGTGGCAC TGGAGGGTCA TACCTGGACA TTTAATCCTT TGAGCGTAAA CTTAGTACCT 
CCGAGCACAG ATCCAGCCAA CATGATGCAG GGTGAAGCCA ATAGACGTAG GGATCGATCA 
GTTGGCTCGA CACTTAGTGA GGGTGCGGAT ATCGAGGTGG AGAAGCTTCT GCGTGATGCA 
GCACGTGGTG AAGCTGGTGT TGCTGCCGTA CGCGAATTCC TCCGAAAATA TCCAGGCAAA 
GTGGATGCTC GAGCAGGAGG TCCTGGTGGT GGTAAAAAAA CCTGTCTCCA AGTAGCCGCT 
CACCAGGGTC AACGTGAACT TTGTAATCTT CTTCTGGATG CTGGAGCTTC CCTTCGTGCT 
GTTGACGAAG ATGGTGACAC TCCGTTGCAC TATGCTGCTT TTGGAAACCA ACCGGAGATA 
ATGGAACTTC TTCTTTCTCG CGGTGCAGCA ATAGATGCTG TAAACAATGG TCGTTGCAGT 
GCGTTACACG TGGCTGTGAA TAAGCAGCAC GCTGCTTGCG TGAGAGCCTT GCTACGTCAT 
GGATGTGATG TAAACTTACA GGACTCTTAC GGCGACACAG CACTGCACGA TGCGATTGGT 
AAGGATGCCC TGGAGATAGT CGATGCACTT TGTTCCTGCG AGAGGACCAA CTTTACACTG 
CGCAACAAGC GTGGTTTTAA TGTTCTGCAT CACGCTGCTC TTAAGGGAAA CGCTCACGCA 
ACGGAGCGAC TGGTAGCCAG AGCCAGACAG CTCGTGGACG TAAAGAAAGA GGACGGATTC 
GCTGCACTTC ATCTTGCTAC ATTGAACGGG CATCGCGAAG TAGCAGCTAT ATTACTTTCC 
CAAAATGGTG GTCGTGCCAC GATTGATCTG TGGAATAAAC GAAGGCAGAC ACCCTTGCAT 
TTAGCCACAG CTCAGGGTCA TTGGGCCCTG GTCGAACTTC TCATCAATCA CAATGCGGAT 
CTCGCGGCTA CCGATGAGGA CGGAGATACC GTTCTGCATC TTGCTATTTC GAAATGCAGA 
CACCAGCCAG CTACTGTAGC AACTCCAGAA AGTAGTCGAG ACTCGCCCTT TATTCATGCG 
ATCTGGCAGG ACCTTGCGAG ACAAGGTGCA AAGACTGAAA TGGCTCTTGC TTGTTTTCTT 
GTCAGTGTCG ATACCACTGA ACTCCTATTA GATCAAACTA GAAACTCGAA AGGGAAGACG 
GCTCTAGATC TTTTAGAGAC GGATCCAGAC GCTCCTGCAC TGACTGCTCT TTTACTCTCT 
TATCAACCAC ACAGACGCAA TAGCAACGAC TTGGTCCCGT CCGAGACTGT TTCATCCGGC 
GGCAGTGTAG AGACACCGAC ATACGAGATT GAAACTATAT CAGAACCGGA AGTCACGAAT 
GAAGAATCGA GAAAGCCTCC TAGGAGTTCC GGTGGCTCTG ACCAGTGCCG TGGATGCTCT 
GCGTTACTGA CTCAGGGTGT AGAAGAAACT AGAATAACAG CCGGAGGCAG TGAGACATTC 
GTAGGCTGCA TACACTGTGG TCACACGGTA CCAAAATCTC AGATTAATCG AGATGGCCAT 
GGATTCAAAG ATGACATGGC AGAGGGCGAA GACCGAAAAA GGGAGGGTCT AGACGATAGA 
AGAAATGAAG ACAAAGCAAA GGACAAGGAT ATAGAAAAGG AAAAAGATCT GGAACGGCTA 
CGCTACTTAG AGACAAGGGT AGCCGATCTC GAGGAAGCGA ACATGTGCAG CATCTGCATG 
GAGCGTCGTA GAAACGTCGC TTTCCTCTGT GGTCACGGCG CATGCGAACA CTGTGCCGCG 
CCCTTGAAAA CCTGCCACAT GTGTCGCAAG ACTATAACCA AAAAGATCAA TCTCTATTGA