LOC107264516 gene cDNA ORF clone, Cephus cinctus(wheat stem sawfly)

mRNA	Protein	Name
XM_015732889.1	XP_015588375.1	protein polybromo-1 isoform X1
XM_015733057.1	XP_015588543.1	protein polybromo-1 isoform X3
XM_015733143.1	XP_015588629.1	protein polybromo-1 isoform X4
XM_015732977.2	XP_015588463.1	protein polybromo-1 isoform X2
XM_015733229.2	XP_015588715.1	protein polybromo-1 isoform X5
XM_015733309.2	XP_015588795.1	protein polybromo-1 isoform X7
XM_015733057.2	XP_015588543.1	protein polybromo-1 isoform X3
XM_015733143.2	XP_015588629.1	protein polybromo-1 isoform X4
XM_025081666.1	XP_024937434.1	protein polybromo-1 isoform X8
XM_015732889.2	XP_015588375.1	protein polybromo-1 isoform X1
XM_015733229.1	XP_015588715.1	protein polybromo-1 isoform X5
XM_015733309.1	XP_015588795.1	protein polybromo-1 isoform X6
XM_015732977.1	XP_015588463.1	protein polybromo-1 isoform X2
XM_025081589.1	XP_024937357.1	protein polybromo-1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe309778	XM_025081589.1 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe309779	XM_025081666.1 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCe76237	XM_015732889.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76238	XM_015732977.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76239	XM_015733057.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76240	XM_015733143.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76241	XM_015733229.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76242	XM_015733309.2 Latest version!	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OCe76239	XM_015733057.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76240	XM_015733143.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76237	XM_015732889.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76241	XM_015733229.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe76242	XM_015733309.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe76238	XM_015732977.1	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

LOC107264516 ( XM_025081589.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_024937357.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe309778
Clone ID Related Accession (Same CDS sequence)	XM_025081589.1
Accession Version	XM_025081589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024937357.1
CDS	176..5287
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_025081589.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025081589.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_025081666.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_024937434.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe309779
Clone ID Related Accession (Same CDS sequence)	XM_025081666.1
Accession Version	XM_025081666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGTGGCG GAAGTTGCCA GCTGGTGAGA AACAGGCGTG GGAGGAACGT 
GCAGTCAAGA TGAACGAAGA TGGTCCGAAC GGTACTGGAG CTGGTGGTGG TGCTGGGGGT 
GGCGGAGCAG GTGGCGGTGG CGGCGCCGGA AGTGTCGCTG GTGGCGGTAC CGGTGGCTTC 
TTCGGAACTG GAGGAACTGG TACTGGTGGT GGAGGCGTCG GTGGTGCCGG CGCTGGTGGC 
GGCGGCAACC AATCGACGAA AGGAACAAGT GTTAATGCTG CCAACTCTTC ACCAGATCAG 
GTCTACGAAT GTTGCTGGGA CAATTGTGAC TGGCAATTTG AGGACATGAC GGATTGCATC 
GATCACTGTA TTGCTGAGCA AAGTGGACAC GTGCAGAACT CTTTCTTAAA CGCATCCAGT 
GACGTGGAGT TTCAGTGCCA ATGGCGCGGA TGCGGAAGAA CAAAGAAAAC TGTACCTCCG 
TTTCCAAGTG TACAACGACT CGCAAGGCAC GTCAAAGAGG TTCACATTCT CAAAGGGAAT 
GGACGCATTG TCCCCCCTTC TGAGAGAAGC AAAAATTACA TGGCTTCGAA AGGTCCTACG 
ATACTACCCC CCATGGAAGC TGAAACAATA GCAACAGCAA CACAAACCAG CGGTGTTCCA 
GCTACTAAAC AACCGGAGCC GTTATTTATT GCTGTGCCGC CACGACCTAG TCGTGTCTTA 
CATTCAGATG CATATTTACG GTATATCGAG GGACTGAACG TGGATAATCG CTACATATCA 
AATTGGGATA AACAAATGAA TGCCACTGCA GAAAACACGC AAATTCCGGA TCTGACAAAA 
CTTCCAGCTG AGTGGCTGGG CAATGGCGTC GGCAATCATG GCAACGTCGT GAATGCCTTG 
TGGACTCTCA GGGACATGAT GATGCGCGAT GTGTTGGCCA TCAATAAAAC TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024937434.1
CDS	176..5032
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_025081666.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025081666.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGTGGCG GAAGTTGCCA GCTGGTGAGA AACAGGCGTG GGAGGAACGT 
GCAGTCAAGA TGAACGAAGA TGGTCCGAAC GGTACTGGAG CTGGTGGTGG TGCTGGGGGT 
GGCGGAGCAG GTGGCGGTGG CGGCGCCGGA AGTGTCGCTG GTGGCGGTAC CGGTGGCTTC 
TTCGGAACTG GAGGAACTGG TACTGGTGGT GGAGGCGTCG GTGGTGCCGG CGCTGGTGGC 
GGCGGCAACC AATCGACGAA AGGAACAAGT GTTAATGCTG CCAACTCTTC ACCAGATCAG 
GTCTACGAAT GTTGCTGGGA CAATTGTGAC TGGCAATTTG AGGACATGAC GGATTGCATC 
GATCACTGTA TTGCTGAGCA AAGTGGACAC GTGCAGAACT CTTTCTTAAA CGCATCCAGT 
GACGTGGAGT TTCAGTGCCA ATGGCGCGGA TGCGGAAGAA CAAAGAAAAC TGTACCTCCG 
TTTCCAAGTG TACAACGACT CGCAAGGCAC GTCAAAGAGG TTCACATTCT CAAAGGGAAT 
GGACGCATTG TCCCCCCTTC TGAGAGAAGC AAAAATTACA TGGCTTCGAA AGGTCCTACG 
ATACTACCCC CCATGGAAGC TGAAACAATA GCAACAGCAA CACAAACCAG CGGTGTTCCA 
GCTACTAAAC AACCGGAGCC GTTATTTATT GCTGTGCCGC CACGACCTAG TCGTGTCTTA 
CATTCAGATG CATATTTACG GTATATCGAG GGACTGAACG TGGATAATCG CTACATATCA 
AATTGGGATA AACAAATGAA TGCCACTGCA GAAAACACGC AAATTCCGGA TCTGACAAAA 
CTTCCAGCTG AGTGGCTGGG CAATGGCGTC GGCAATCATG GCAACGTCGT GAATGCCTTG 
TGGACTCTCA GGGACATGAT GATGCGCGAT GTGTTGGCCA TCAATAAAAC TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015732889.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588375.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe76237
Clone ID Related Accession (Same CDS sequence)	XM_015732889.1 , XM_015732889.2
Accession Version	XM_015732889.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG AAACAATAGC AACAGCAACA 
CAAACCAGCG GTGTTCCAGC TACTAAACAA CCGGAGCCGT TATTTATTGC TGTGCCGCCA 
CGACCTAGTC GTGTCTTACA TTCAGATGCA TATTTACGGT ATATCGAGGG ACTGAACGTG 
GATAATCGCT ACATATCAAA TTGGGATAAA CAAATGAATG CCACTGCAGA AAACACGCAA 
ATTCCGGATC TGACAAAACT TCCAGCTGAG TGGCTGGGCA ATGGCGTCGG CAATCATGGC 
AACGTCGTGA ATGCCTTGTG GACTCTCAGG GACATGATGA TGCGCGATGT GTTGGCCATC 
AATAAAACTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588375.1
CDS	157..5391
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015732889.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015732889.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG AAACAATAGC AACAGCAACA 
CAAACCAGCG GTGTTCCAGC TACTAAACAA CCGGAGCCGT TATTTATTGC TGTGCCGCCA 
CGACCTAGTC GTGTCTTACA TTCAGATGCA TATTTACGGT ATATCGAGGG ACTGAACGTG 
GATAATCGCT ACATATCAAA TTGGGATAAA CAAATGAATG CCACTGCAGA AAACACGCAA 
ATTCCGGATC TGACAAAACT TCCAGCTGAG TGGCTGGGCA ATGGCGTCGG CAATCATGGC 
AACGTCGTGA ATGCCTTGTG GACTCTCAGG GACATGATGA TGCGCGATGT GTTGGCCATC 
AATAAAACTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015732889.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588375.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.

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CloneID	OCe76237
Clone ID Related Accession (Same CDS sequence)	XM_015732889.1 , XM_015732889.2
Accession Version	XM_015732889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015732889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG AAACAATAGC AACAGCAACA 
CAAACCAGCG GTGTTCCAGC TACTAAACAA CCGGAGCCGT TATTTATTGC TGTGCCGCCA 
CGACCTAGTC GTGTCTTACA TTCAGATGCA TATTTACGGT ATATCGAGGG ACTGAACGTG 
GATAATCGCT ACATATCAAA TTGGGATAAA CAAATGAATG CCACTGCAGA AAACACGCAA 
ATTCCGGATC TGACAAAACT TCCAGCTGAG TGGCTGGGCA ATGGCGTCGG CAATCATGGC 
AACGTCGTGA ATGCCTTGTG GACTCTCAGG GACATGATGA TGCGCGATGT GTTGGCCATC 
AATAAAACTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588375.1
CDS	175..5409
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015732889.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015732889.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG AAACAATAGC AACAGCAACA 
CAAACCAGCG GTGTTCCAGC TACTAAACAA CCGGAGCCGT TATTTATTGC TGTGCCGCCA 
CGACCTAGTC GTGTCTTACA TTCAGATGCA TATTTACGGT ATATCGAGGG ACTGAACGTG 
GATAATCGCT ACATATCAAA TTGGGATAAA CAAATGAATG CCACTGCAGA AAACACGCAA 
ATTCCGGATC TGACAAAACT TCCAGCTGAG TGGCTGGGCA ATGGCGTCGG CAATCATGGC 
AACGTCGTGA ATGCCTTGTG GACTCTCAGG GACATGATGA TGCGCGATGT GTTGGCCATC 
AATAAAACTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015732977.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588463.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.

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CloneID	OCe76238
Clone ID Related Accession (Same CDS sequence)	XM_015732977.2 , XM_015732977.1
Accession Version	XM_015732977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015732977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC TGTGAAGATG GTCGACAACC AATGTTAATG 
TTCATGGAGA AACCATCGAA AAAGATCTAT CCAGATTATT ATCAAGTGAT TGCCGAGCCA 
ATTGACATGC TACAGATAGA AGCAAACATC AAGGCTGAAA AGTACCAAAG TGAAAATGAA 
CTGATACAAG ATTTCAAATT GATGTTTAAC AACTGTCGTC AGTACAACGA GGAAGGTTCG 
TTAATTTACG AGGATGCAAA TACTTTGGAA AGAGTTTTGA TGGACAAAGT GAAAGAGTTG 
GGTCCACTGC CGGAAATAAT TAAGACAATT AAATCGACAG CATCGACGCC TACAAGGAAC 
GTTGGGAGAC CGAAGAAAGT TGTACCTTTA CATTTACAAA AATTGCGTAC CCTCTATGAC 
ACTCTCAAAG ATTGTCGTGA TGCCAAGGGT CGTCAGTTGT CCTTAATATT TATGAAATTA 
CCAAATAAAA ATGAGTATCC CGATTATTAT GAGGTCATAA AACAGCCAAT GAACATGGAG 
AAAATTGCCT CCACTTTAAA GAACAACGGA TATGAAACTT TGGATGAACT GGTGTCAGAC 
TTTGTATTGA TGTTTGATAA CGCCTGCAAA TACAACGAGC CCGATTCACA AATTTATAAG 
GATGCCCTAA TTTTGCAACG ATTGGTACTA CAAACAAAGC TACAACTGAG CGAGGACGAC 
GAGAGTGTTC CGGATGTGGC AGCTGCCGTT CAGGAAATAC TTGCAACGAT ATTCACTGCC 
CTGTATAATC ATCAAGACGA GGAGGGTCGT TGTTACTCAG ACTCCATGGC AGAACTTCCG 
GAGCACGACA TAATTGACGG CAAGAAAGTG CGAGGTCTAT CCTTGGATCT GATAAAACGA 
AGATTGGACC GTGGAGCGTA CAAAAGATTA GATCGTTTCC AAGAGGATGT CTTCTCCTGC 
CTGGAAAGAG CACGCAGGTT GTCGCGAAGC GATTCTCAGC CTTTCGAAGA CAGCGTCGAG 
CTACAGGCGT TCTTTCTGCG TACTCGGGAC GAGGTCACTC GTTGCGGTGA CCTTCTGCAC 
TCTCCCGCTC TCAACTACAC TCTGCTTGAT TTGTCCAGTC AAGTTGTTGA GCTGAAGCGC 
ATAAAACTTC AGCAGGAGTT GTCGCTGCCG AACGAGGAGG AGAGTTGCGA CGGCAACGAC 
AGCGTTAAGG AGTCGGCGAC AACACTTCTG ACACCCGTTG CTCTACCATC CGGAAGTACA 
ACGACAGCGA CTGCTCCAAC AGCACTGAGT TCCGTAACGA TACCAACGAC AGGAGCAACG 
GCAACTGGTG TATCAGCTGC TGGCGGCAAC GGTGTAGCTG CCGACGGTGG CAACTCGATG 
AGCTTCAACC AGGAAGTTTA TAGAGCCGGG GACTTTGCTT ACGTTGAGCC ATCGGAACGC 
GGCATGGAAC TGAGCGTCGT TCTCATTGAG CGTCTTTGGA CAAATGCAGA AGGGCAAGAG 
ATGCTTTATG GAAATTTATT CTATAGACCT AGTGAAACGT ATCACGTGGC CTCTAGAAAG 
TTTCTCGACA AGGAACTCTT CAAGAGCGAT GCTCACGTTG CTGTGCCTCT GGGTAAAGTA 
GCTGGTCGTT GCTGCGTCCT GAGCGTCAAG GACTACTTTA GAATGCAGCC CGAAGGATTT 
CCCGAGAAGG ATGTCTACGT CTGCGAGTCC CGATACTCGA CGAAGGCTAG AGCCTTCAAA 
AAAATAAAAG TTTGGAACTT CGATCCGGAT CATCTGAAGC TGGTGCCAAG AGAACGAGCC 
TTGGAACCAA AACGTGTAAT TTCGGTTTAC AAGGAGCGAC TCGAGAAGCA TAAGGAAGAG 
ATTGCTGAGC TGGAGGAGGG TGAAAAACTC ATCGAAAAGG AGAGACCGAA TGTGATAATG 
TTCAATCCGG ACGACACCGA GAACACGTAT TACGAGCAGT ACAACACGTG CACGGGAACC 
GTGAAAACCG GTGACTTTGT CTACGTCGCT ACCGATGGCG GAAGACAACA AATTGCGCAG 
ATCGATGCCA TCTGGTCGAC GAAAGACGGA AAGTGTTACT TCAAGGGTCC CTGGTTATTG 
ACTCCTACGG AAATTCCTCA CGCACCCACC AAACTCTTCT ACAAGCAGGA ACTCTTCTTG 
TCCACGGTCG ATGGTACACA TCCGATCGTC GCCATTGTTG GAAAGTGTGC TGTATTGGAC 
TACGGTGAAT TTGTATGCAG TCGTCCAACG GAAATCCCGG AAGACGACGT ATTCATATGC 
GAGTCGACTT ACGATGAGAG CAAAAATTTG ATAAGGAAGC TCAGTGCGGA TGGCTTGAAG 
AAATTTAATC ATACCTCGGC AGTTACGGAC GACGAGATTT ACTTTTTCCG TCGGCCCATC 
AATCCGGCAA AGGTGCCTAG CGACGTCGCA CAGACGCCAT CCCAAGCCAA GTCGGTTGTA 
TCTACTATGG CTCACTTTGA AATGGAGGCA TCGCCGTTGC TACCGAAGCT GGAGCCAGAT 
GTGCTCGGGA TGGGAGTGGG ATTGGGAGTG GGAGTGGGAG TGGGTGAGGA CAGCATGGAT 
GGCGGTGGAC CACCGTCCGT CGGATCCAGC GAAGCTCAGC CGGTGCTCCA ATGCTCCAAT 
GCTCAGACAC CTGTATCGAC AAAAAAGAAA TCGACGGGTA AGAAATTAGT TACGGGCTAT 
ATTTTGTATT CGAGTAAAAT GCGAACGCAG ATTACGCAAA ACAATCCTGA ATCGTCCTTC 
GGAGAGATTA GCCGAATTGT TGGCAATGAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588463.1
CDS	176..5407
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015732977.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015732977.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC TGTGAAGATG GTCGACAACC AATGTTAATG 
TTCATGGAGA AACCATCGAA AAAGATCTAT CCAGATTATT ATCAAGTGAT TGCCGAGCCA 
ATTGACATGC TACAGATAGA AGCAAACATC AAGGCTGAAA AGTACCAAAG TGAAAATGAA 
CTGATACAAG ATTTCAAATT GATGTTTAAC AACTGTCGTC AGTACAACGA GGAAGGTTCG 
TTAATTTACG AGGATGCAAA TACTTTGGAA AGAGTTTTGA TGGACAAAGT GAAAGAGTTG 
GGTCCACTGC CGGAAATAAT TAAGACAATT AAATCGACAG CATCGACGCC TACAAGGAAC 
GTTGGGAGAC CGAAGAAAGT TGTACCTTTA CATTTACAAA AATTGCGTAC CCTCTATGAC 
ACTCTCAAAG ATTGTCGTGA TGCCAAGGGT CGTCAGTTGT CCTTAATATT TATGAAATTA 
CCAAATAAAA ATGAGTATCC CGATTATTAT GAGGTCATAA AACAGCCAAT GAACATGGAG 
AAAATTGCCT CCACTTTAAA GAACAACGGA TATGAAACTT TGGATGAACT GGTGTCAGAC 
TTTGTATTGA TGTTTGATAA CGCCTGCAAA TACAACGAGC CCGATTCACA AATTTATAAG 
GATGCCCTAA TTTTGCAACG ATTGGTACTA CAAACAAAGC TACAACTGAG CGAGGACGAC 
GAGAGTGTTC CGGATGTGGC AGCTGCCGTT CAGGAAATAC TTGCAACGAT ATTCACTGCC 
CTGTATAATC ATCAAGACGA GGAGGGTCGT TGTTACTCAG ACTCCATGGC AGAACTTCCG 
GAGCACGACA TAATTGACGG CAAGAAAGTG CGAGGTCTAT CCTTGGATCT GATAAAACGA 
AGATTGGACC GTGGAGCGTA CAAAAGATTA GATCGTTTCC AAGAGGATGT CTTCTCCTGC 
CTGGAAAGAG CACGCAGGTT GTCGCGAAGC GATTCTCAGC CTTTCGAAGA CAGCGTCGAG 
CTACAGGCGT TCTTTCTGCG TACTCGGGAC GAGGTCACTC GTTGCGGTGA CCTTCTGCAC 
TCTCCCGCTC TCAACTACAC TCTGCTTGAT TTGTCCAGTC AAGTTGTTGA GCTGAAGCGC 
ATAAAACTTC AGCAGGAGTT GTCGCTGCCG AACGAGGAGG AGAGTTGCGA CGGCAACGAC 
AGCGTTAAGG AGTCGGCGAC AACACTTCTG ACACCCGTTG CTCTACCATC CGGAAGTACA 
ACGACAGCGA CTGCTCCAAC AGCACTGAGT TCCGTAACGA TACCAACGAC AGGAGCAACG 
GCAACTGGTG TATCAGCTGC TGGCGGCAAC GGTGTAGCTG CCGACGGTGG CAACTCGATG 
AGCTTCAACC AGGAAGTTTA TAGAGCCGGG GACTTTGCTT ACGTTGAGCC ATCGGAACGC 
GGCATGGAAC TGAGCGTCGT TCTCATTGAG CGTCTTTGGA CAAATGCAGA AGGGCAAGAG 
ATGCTTTATG GAAATTTATT CTATAGACCT AGTGAAACGT ATCACGTGGC CTCTAGAAAG 
TTTCTCGACA AGGAACTCTT CAAGAGCGAT GCTCACGTTG CTGTGCCTCT GGGTAAAGTA 
GCTGGTCGTT GCTGCGTCCT GAGCGTCAAG GACTACTTTA GAATGCAGCC CGAAGGATTT 
CCCGAGAAGG ATGTCTACGT CTGCGAGTCC CGATACTCGA CGAAGGCTAG AGCCTTCAAA 
AAAATAAAAG TTTGGAACTT CGATCCGGAT CATCTGAAGC TGGTGCCAAG AGAACGAGCC 
TTGGAACCAA AACGTGTAAT TTCGGTTTAC AAGGAGCGAC TCGAGAAGCA TAAGGAAGAG 
ATTGCTGAGC TGGAGGAGGG TGAAAAACTC ATCGAAAAGG AGAGACCGAA TGTGATAATG 
TTCAATCCGG ACGACACCGA GAACACGTAT TACGAGCAGT ACAACACGTG CACGGGAACC 
GTGAAAACCG GTGACTTTGT CTACGTCGCT ACCGATGGCG GAAGACAACA AATTGCGCAG 
ATCGATGCCA TCTGGTCGAC GAAAGACGGA AAGTGTTACT TCAAGGGTCC CTGGTTATTG 
ACTCCTACGG AAATTCCTCA CGCACCCACC AAACTCTTCT ACAAGCAGGA ACTCTTCTTG 
TCCACGGTCG ATGGTACACA TCCGATCGTC GCCATTGTTG GAAAGTGTGC TGTATTGGAC 
TACGGTGAAT TTGTATGCAG TCGTCCAACG GAAATCCCGG AAGACGACGT ATTCATATGC 
GAGTCGACTT ACGATGAGAG CAAAAATTTG ATAAGGAAGC TCAGTGCGGA TGGCTTGAAG 
AAATTTAATC ATACCTCGGC AGTTACGGAC GACGAGATTT ACTTTTTCCG TCGGCCCATC 
AATCCGGCAA AGGTGCCTAG CGACGTCGCA CAGACGCCAT CCCAAGCCAA GTCGGTTGTA 
TCTACTATGG CTCACTTTGA AATGGAGGCA TCGCCGTTGC TACCGAAGCT GGAGCCAGAT 
GTGCTCGGGA TGGGAGTGGG ATTGGGAGTG GGAGTGGGAG TGGGTGAGGA CAGCATGGAT 
GGCGGTGGAC CACCGTCCGT CGGATCCAGC GAAGCTCAGC CGGTGCTCCA ATGCTCCAAT 
GCTCAGACAC CTGTATCGAC AAAAAAGAAA TCGACGGGTA AGAAATTAGT TACGGGCTAT 
ATTTTGTATT CGAGTAAAAT GCGAACGCAG ATTACGCAAA ACAATCCTGA ATCGTCCTTC 
GGAGAGATTA GCCGAATTGT TGGCAATGAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015732977.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588463.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.

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CloneID	OCe76238
Clone ID Related Accession (Same CDS sequence)	XM_015732977.2 , XM_015732977.1
Accession Version	XM_015732977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC TGTGAAGATG GTCGACAACC AATGTTAATG 
TTCATGGAGA AACCATCGAA AAAGATCTAT CCAGATTATT ATCAAGTGAT TGCCGAGCCA 
ATTGACATGC TACAGATAGA AGCAAACATC AAGGCTGAAA AGTACCAAAG TGAAAATGAA 
CTGATACAAG ATTTCAAATT GATGTTTAAC AACTGTCGTC AGTACAACGA GGAAGGTTCG 
TTAATTTACG AGGATGCAAA TACTTTGGAA AGAGTTTTGA TGGACAAAGT GAAAGAGTTG 
GGTCCACTGC CGGAAATAAT TAAGACAATT AAATCGACAG CATCGACGCC TACAAGGAAC 
GTTGGGAGAC CGAAGAAAGT TGTACCTTTA CATTTACAAA AATTGCGTAC CCTCTATGAC 
ACTCTCAAAG ATTGTCGTGA TGCCAAGGGT CGTCAGTTGT CCTTAATATT TATGAAATTA 
CCAAATAAAA ATGAGTATCC CGATTATTAT GAGGTCATAA AACAGCCAAT GAACATGGAG 
AAAATTGCCT CCACTTTAAA GAACAACGGA TATGAAACTT TGGATGAACT GGTGTCAGAC 
TTTGTATTGA TGTTTGATAA CGCCTGCAAA TACAACGAGC CCGATTCACA AATTTATAAG 
GATGCCCTAA TTTTGCAACG ATTGGTACTA CAAACAAAGC TACAACTGAG CGAGGACGAC 
GAGAGTGTTC CGGATGTGGC AGCTGCCGTT CAGGAAATAC TTGCAACGAT ATTCACTGCC 
CTGTATAATC ATCAAGACGA GGAGGGTCGT TGTTACTCAG ACTCCATGGC AGAACTTCCG 
GAGCACGACA TAATTGACGG CAAGAAAGTG CGAGGTCTAT CCTTGGATCT GATAAAACGA 
AGATTGGACC GTGGAGCGTA CAAAAGATTA GATCGTTTCC AAGAGGATGT CTTCTCCTGC 
CTGGAAAGAG CACGCAGGTT GTCGCGAAGC GATTCTCAGC CTTTCGAAGA CAGCGTCGAG 
CTACAGGCGT TCTTTCTGCG TACTCGGGAC GAGGTCACTC GTTGCGGTGA CCTTCTGCAC 
TCTCCCGCTC TCAACTACAC TCTGCTTGAT TTGTCCAGTC AAGTTGTTGA GCTGAAGCGC 
ATAAAACTTC AGCAGGAGTT GTCGCTGCCG AACGAGGAGG AGAGTTGCGA CGGCAACGAC 
AGCGTTAAGG AGTCGGCGAC AACACTTCTG ACACCCGTTG CTCTACCATC CGGAAGTACA 
ACGACAGCGA CTGCTCCAAC AGCACTGAGT TCCGTAACGA TACCAACGAC AGGAGCAACG 
GCAACTGGTG TATCAGCTGC TGGCGGCAAC GGTGTAGCTG CCGACGGTGG CAACTCGATG 
AGCTTCAACC AGGAAGTTTA TAGAGCCGGG GACTTTGCTT ACGTTGAGCC ATCGGAACGC 
GGCATGGAAC TGAGCGTCGT TCTCATTGAG CGTCTTTGGA CAAATGCAGA AGGGCAAGAG 
ATGCTTTATG GAAATTTATT CTATAGACCT AGTGAAACGT ATCACGTGGC CTCTAGAAAG 
TTTCTCGACA AGGAACTCTT CAAGAGCGAT GCTCACGTTG CTGTGCCTCT GGGTAAAGTA 
GCTGGTCGTT GCTGCGTCCT GAGCGTCAAG GACTACTTTA GAATGCAGCC CGAAGGATTT 
CCCGAGAAGG ATGTCTACGT CTGCGAGTCC CGATACTCGA CGAAGGCTAG AGCCTTCAAA 
AAAATAAAAG TTTGGAACTT CGATCCGGAT CATCTGAAGC TGGTGCCAAG AGAACGAGCC 
TTGGAACCAA AACGTGTAAT TTCGGTTTAC AAGGAGCGAC TCGAGAAGCA TAAGGAAGAG 
ATTGCTGAGC TGGAGGAGGG TGAAAAACTC ATCGAAAAGG AGAGACCGAA TGTGATAATG 
TTCAATCCGG ACGACACCGA GAACACGTAT TACGAGCAGT ACAACACGTG CACGGGAACC 
GTGAAAACCG GTGACTTTGT CTACGTCGCT ACCGATGGCG GAAGACAACA AATTGCGCAG 
ATCGATGCCA TCTGGTCGAC GAAAGACGGA AAGTGTTACT TCAAGGGTCC CTGGTTATTG 
ACTCCTACGG AAATTCCTCA CGCACCCACC AAACTCTTCT ACAAGCAGGA ACTCTTCTTG 
TCCACGGTCG ATGGTACACA TCCGATCGTC GCCATTGTTG GAAAGTGTGC TGTATTGGAC 
TACGGTGAAT TTGTATGCAG TCGTCCAACG GAAATCCCGG AAGACGACGT ATTCATATGC 
GAGTCGACTT ACGATGAGAG CAAAAATTTG ATAAGGAAGC TCAGTGCGGA TGGCTTGAAG 
AAATTTAATC ATACCTCGGC AGTTACGGAC GACGAGATTT ACTTTTTCCG TCGGCCCATC 
AATCCGGCAA AGGTGCCTAG CGACGTCGCA CAGACGCCAT CCCAAGCCAA GTCGGTTGTA 
TCTACTATGG CTCACTTTGA AATGGAGGCA TCGCCGTTGC TACCGAAGCT GGAGCCAGAT 
GTGCTCGGGA TGGGAGTGGG ATTGGGAGTG GGAGTGGGAG TGGGTGAGGA CAGCATGGAT 
GGCGGTGGAC CACCGTCCGT CGGATCCAGC GAAGCTCAGC CGGTGCTCCA ATGCTCCAAT 
GCTCAGACAC CTGTATCGAC AAAAAAGAAA TCGACGGGTA AGAAATTAGT TACGGGCTAT 
ATTTTGTATT CGAGTAAAAT GCGAACGCAG ATTACGCAAA ACAATCCTGA ATCGTCCTTC 
GGAGAGATTA GCCGAATTGT TGGCAATGAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588463.1
CDS	157..5388
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015732977.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015732977.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC TGTGAAGATG GTCGACAACC AATGTTAATG 
TTCATGGAGA AACCATCGAA AAAGATCTAT CCAGATTATT ATCAAGTGAT TGCCGAGCCA 
ATTGACATGC TACAGATAGA AGCAAACATC AAGGCTGAAA AGTACCAAAG TGAAAATGAA 
CTGATACAAG ATTTCAAATT GATGTTTAAC AACTGTCGTC AGTACAACGA GGAAGGTTCG 
TTAATTTACG AGGATGCAAA TACTTTGGAA AGAGTTTTGA TGGACAAAGT GAAAGAGTTG 
GGTCCACTGC CGGAAATAAT TAAGACAATT AAATCGACAG CATCGACGCC TACAAGGAAC 
GTTGGGAGAC CGAAGAAAGT TGTACCTTTA CATTTACAAA AATTGCGTAC CCTCTATGAC 
ACTCTCAAAG ATTGTCGTGA TGCCAAGGGT CGTCAGTTGT CCTTAATATT TATGAAATTA 
CCAAATAAAA ATGAGTATCC CGATTATTAT GAGGTCATAA AACAGCCAAT GAACATGGAG 
AAAATTGCCT CCACTTTAAA GAACAACGGA TATGAAACTT TGGATGAACT GGTGTCAGAC 
TTTGTATTGA TGTTTGATAA CGCCTGCAAA TACAACGAGC CCGATTCACA AATTTATAAG 
GATGCCCTAA TTTTGCAACG ATTGGTACTA CAAACAAAGC TACAACTGAG CGAGGACGAC 
GAGAGTGTTC CGGATGTGGC AGCTGCCGTT CAGGAAATAC TTGCAACGAT ATTCACTGCC 
CTGTATAATC ATCAAGACGA GGAGGGTCGT TGTTACTCAG ACTCCATGGC AGAACTTCCG 
GAGCACGACA TAATTGACGG CAAGAAAGTG CGAGGTCTAT CCTTGGATCT GATAAAACGA 
AGATTGGACC GTGGAGCGTA CAAAAGATTA GATCGTTTCC AAGAGGATGT CTTCTCCTGC 
CTGGAAAGAG CACGCAGGTT GTCGCGAAGC GATTCTCAGC CTTTCGAAGA CAGCGTCGAG 
CTACAGGCGT TCTTTCTGCG TACTCGGGAC GAGGTCACTC GTTGCGGTGA CCTTCTGCAC 
TCTCCCGCTC TCAACTACAC TCTGCTTGAT TTGTCCAGTC AAGTTGTTGA GCTGAAGCGC 
ATAAAACTTC AGCAGGAGTT GTCGCTGCCG AACGAGGAGG AGAGTTGCGA CGGCAACGAC 
AGCGTTAAGG AGTCGGCGAC AACACTTCTG ACACCCGTTG CTCTACCATC CGGAAGTACA 
ACGACAGCGA CTGCTCCAAC AGCACTGAGT TCCGTAACGA TACCAACGAC AGGAGCAACG 
GCAACTGGTG TATCAGCTGC TGGCGGCAAC GGTGTAGCTG CCGACGGTGG CAACTCGATG 
AGCTTCAACC AGGAAGTTTA TAGAGCCGGG GACTTTGCTT ACGTTGAGCC ATCGGAACGC 
GGCATGGAAC TGAGCGTCGT TCTCATTGAG CGTCTTTGGA CAAATGCAGA AGGGCAAGAG 
ATGCTTTATG GAAATTTATT CTATAGACCT AGTGAAACGT ATCACGTGGC CTCTAGAAAG 
TTTCTCGACA AGGAACTCTT CAAGAGCGAT GCTCACGTTG CTGTGCCTCT GGGTAAAGTA 
GCTGGTCGTT GCTGCGTCCT GAGCGTCAAG GACTACTTTA GAATGCAGCC CGAAGGATTT 
CCCGAGAAGG ATGTCTACGT CTGCGAGTCC CGATACTCGA CGAAGGCTAG AGCCTTCAAA 
AAAATAAAAG TTTGGAACTT CGATCCGGAT CATCTGAAGC TGGTGCCAAG AGAACGAGCC 
TTGGAACCAA AACGTGTAAT TTCGGTTTAC AAGGAGCGAC TCGAGAAGCA TAAGGAAGAG 
ATTGCTGAGC TGGAGGAGGG TGAAAAACTC ATCGAAAAGG AGAGACCGAA TGTGATAATG 
TTCAATCCGG ACGACACCGA GAACACGTAT TACGAGCAGT ACAACACGTG CACGGGAACC 
GTGAAAACCG GTGACTTTGT CTACGTCGCT ACCGATGGCG GAAGACAACA AATTGCGCAG 
ATCGATGCCA TCTGGTCGAC GAAAGACGGA AAGTGTTACT TCAAGGGTCC CTGGTTATTG 
ACTCCTACGG AAATTCCTCA CGCACCCACC AAACTCTTCT ACAAGCAGGA ACTCTTCTTG 
TCCACGGTCG ATGGTACACA TCCGATCGTC GCCATTGTTG GAAAGTGTGC TGTATTGGAC 
TACGGTGAAT TTGTATGCAG TCGTCCAACG GAAATCCCGG AAGACGACGT ATTCATATGC 
GAGTCGACTT ACGATGAGAG CAAAAATTTG ATAAGGAAGC TCAGTGCGGA TGGCTTGAAG 
AAATTTAATC ATACCTCGGC AGTTACGGAC GACGAGATTT ACTTTTTCCG TCGGCCCATC 
AATCCGGCAA AGGTGCCTAG CGACGTCGCA CAGACGCCAT CCCAAGCCAA GTCGGTTGTA 
TCTACTATGG CTCACTTTGA AATGGAGGCA TCGCCGTTGC TACCGAAGCT GGAGCCAGAT 
GTGCTCGGGA TGGGAGTGGG ATTGGGAGTG GGAGTGGGAG TGGGTGAGGA CAGCATGGAT 
GGCGGTGGAC CACCGTCCGT CGGATCCAGC GAAGCTCAGC CGGTGCTCCA ATGCTCCAAT 
GCTCAGACAC CTGTATCGAC AAAAAAGAAA TCGACGGGTA AGAAATTAGT TACGGGCTAT 
ATTTTGTATT CGAGTAAAAT GCGAACGCAG ATTACGCAAA ACAATCCTGA ATCGTCCTTC 
GGAGAGATTA GCCGAATTGT TGGCAATGAG TGGCGGAAGT TGCCAGCTGG TGAGAAACAG 
GCGTGGGAGG AACGTGCAGT CAAGATGAAC GAAGATGGTC CGAACGGTAC TGGAGCTGGT 
GGTGGTGCTG GGGGTGGCGG AGCAGGTGGC GGTGGCGGCG CCGGAAGTGT CGCTGGTGGC 
GGTACCGGTG GCTTCTTCGG AACTGGAGGA ACTGGTACTG GTGGTGGAGG CGTCGGTGGT 
GCCGGCGCTG GTGGCGGCGG CAACCAATCG ACGAAAGGAA CAAGTGTTAA TGCTGCCAAC 
TCTTCACCAG ATCAGGTCTA CGAATGTTGC TGGGACAATT GTGACTGGCA ATTTGAGGAC 
ATGACGGATT GCATCGATCA CTGTATTGCT GAGCAAAGTG GACACGTGCA GAACTCTTTC 
TTAAACGCAT CCAGTGACGT GGAGTTTCAG TGCCAATGGC GCGGATGCGG AAGAACAAAG 
AAAACTGTAC CTCCGTTTCC AAGTGTACAA CGACTCGCAA GGCACGTCAA AGAGGTTCAC 
ATTCTCAAAG GGAATGGACG CATTGTCCCC CCTTCTGAGA GAAGCAAAAA TTACATGGCT 
TCGAAAGGTC CTACGATACT ACCCCCCATG GAAGCTGAAA CAATAGCAAC AGCAACACAA 
ACCAGCGGTG TTCCAGCTAC TAAACAACCG GAGCCGTTAT TTATTGCTGT GCCGCCACGA 
CCTAGTCGTG TCTTACATTC AGATGCATAT TTACGGTATA TCGAGGGACT GAACGTGGAT 
AATCGCTACA TATCAAATTG GGATAAACAA ATGAATGCCA CTGCAGAAAA CACGCAAATT 
CCGGATCTGA CAAAACTTCC AGCTGAGTGG CTGGGCAATG GCGTCGGCAA TCATGGCAAC 
GTCGTGAATG CCTTGTGGAC TCTCAGGGAC ATGATGATGC GCGATGTGTT GGCCATCAAT 
AAAACTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733057.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588543.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.

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CloneID	OCe76239
Clone ID Related Accession (Same CDS sequence)	XM_015733057.1 , XM_015733057.2
Accession Version	XM_015733057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG CAACAGCAAC ACAAACCAGC 
GGTGTTCCAG CTACTAAACA ACCGGAGCCG TTATTTATTG CTGTGCCGCC ACGACCTAGT 
CGTGTCTTAC ATTCAGATGC ATATTTACGG TATATCGAGG GACTGAACGT GGATAATCGC 
TACATATCAA ATTGGGATAA ACAAATGAAT GCCACTGCAG AAAACACGCA AATTCCGGAT 
CTGACAAAAC TTCCAGCTGA GTGGCTGGGC AATGGCGTCG GCAATCATGG CAACGTCGTG 
AATGCCTTGT GGACTCTCAG GGACATGATG ATGCGCGATG TGTTGGCCAT CAATAAAACT 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588543.1
CDS	157..5382
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733057.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733057.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG CAACAGCAAC ACAAACCAGC 
GGTGTTCCAG CTACTAAACA ACCGGAGCCG TTATTTATTG CTGTGCCGCC ACGACCTAGT 
CGTGTCTTAC ATTCAGATGC ATATTTACGG TATATCGAGG GACTGAACGT GGATAATCGC 
TACATATCAA ATTGGGATAA ACAAATGAAT GCCACTGCAG AAAACACGCA AATTCCGGAT 
CTGACAAAAC TTCCAGCTGA GTGGCTGGGC AATGGCGTCG GCAATCATGG CAACGTCGTG 
AATGCCTTGT GGACTCTCAG GGACATGATG ATGCGCGATG TGTTGGCCAT CAATAAAACT 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733057.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588543.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.

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CloneID	OCe76239
Clone ID Related Accession (Same CDS sequence)	XM_015733057.1 , XM_015733057.2
Accession Version	XM_015733057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015733057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG CAACAGCAAC ACAAACCAGC 
GGTGTTCCAG CTACTAAACA ACCGGAGCCG TTATTTATTG CTGTGCCGCC ACGACCTAGT 
CGTGTCTTAC ATTCAGATGC ATATTTACGG TATATCGAGG GACTGAACGT GGATAATCGC 
TACATATCAA ATTGGGATAA ACAAATGAAT GCCACTGCAG AAAACACGCA AATTCCGGAT 
CTGACAAAAC TTCCAGCTGA GTGGCTGGGC AATGGCGTCG GCAATCATGG CAACGTCGTG 
AATGCCTTGT GGACTCTCAG GGACATGATG ATGCGCGATG TGTTGGCCAT CAATAAAACT 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588543.1
CDS	175..5400
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733057.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733057.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGGTA CTGGTGGTGG AGGCGTCGGT 
GGTGCCGGCG CTGGTGGCGG CGGCAACCAA TCGACGAAAG GAACAAGTGT TAATGCTGCC 
AACTCTTCAC CAGATCAGGT CTACGAATGT TGCTGGGACA ATTGTGACTG GCAATTTGAG 
GACATGACGG ATTGCATCGA TCACTGTATT GCTGAGCAAA GTGGACACGT GCAGAACTCT 
TTCTTAAACG CATCCAGTGA CGTGGAGTTT CAGTGCCAAT GGCGCGGATG CGGAAGAACA 
AAGAAAACTG TACCTCCGTT TCCAAGTGTA CAACGACTCG CAAGGCACGT CAAAGAGGTT 
CACATTCTCA AAGGGAATGG ACGCATTGTC CCCCCTTCTG AGAGAAGCAA AAATTACATG 
GCTTCGAAAG GTCCTACGAT ACTACCCCCC ATGGAAGCTG CAACAGCAAC ACAAACCAGC 
GGTGTTCCAG CTACTAAACA ACCGGAGCCG TTATTTATTG CTGTGCCGCC ACGACCTAGT 
CGTGTCTTAC ATTCAGATGC ATATTTACGG TATATCGAGG GACTGAACGT GGATAATCGC 
TACATATCAA ATTGGGATAA ACAAATGAAT GCCACTGCAG AAAACACGCA AATTCCGGAT 
CTGACAAAAC TTCCAGCTGA GTGGCTGGGC AATGGCGTCG GCAATCATGG CAACGTCGTG 
AATGCCTTGT GGACTCTCAG GGACATGATG ATGCGCGATG TGTTGGCCAT CAATAAAACT 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733143.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588629.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.

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CloneID	OCe76240
Clone ID Related Accession (Same CDS sequence)	XM_015733143.1 , XM_015733143.2
Accession Version	XM_015733143.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGAGGC ATCGCCGTTG CTACCGAAGC TGGAGCCAGA TGTGCTCGGG 
ATGGGAGTGG GATTGGGAGT GGGAGTGGGA GTGGGTGAGG ACAGCATGGA TGGCGGTGGA 
CCACCGTCCG TCGGATCCAG CGAAGCTCAG CCGGTGCTCC AATGCTCCAA TGCTCAGACA 
CCTGTATCGA CAAAAAAGAA ATCGACGGGT AAGAAATTAG TTACGGGCTA TATTTTGTAT 
TCGAGTAAAA TGCGAACGCA GATTACGCAA AACAATCCTG AATCGTCCTT CGGAGAGATT 
AGCCGAATTG TTGGCAATGA GTGGCGGAAG TTGCCAGCTG GTGAGAAACA GGCGTGGGAG 
GAACGTGCAG TCAAGATGAA CGAAGATGGT CCGAACGGTA CTGGAGCTGG TGGTGGTGCT 
GGGGGTGGCG GAGCAGGTGG CGGTGGCGGC GCCGGAAGTG TCGCTGGTGG CGGTACCGGT 
GGCTTCTTCG GAACTGGAGG AACTGGTACT GGTGGTGGAG GCGTCGGTGG TGCCGGCGCT 
GGTGGCGGCG GCAACCAATC GACGAAAGGA ACAAGTGTTA ATGCTGCCAA CTCTTCACCA 
GATCAGGTCT ACGAATGTTG CTGGGACAAT TGTGACTGGC AATTTGAGGA CATGACGGAT 
TGCATCGATC ACTGTATTGC TGAGCAAAGT GGACACGTGC AGAACTCTTT CTTAAACGCA 
TCCAGTGACG TGGAGTTTCA GTGCCAATGG CGCGGATGCG GAAGAACAAA GAAAACTGTA 
CCTCCGTTTC CAAGTGTACA ACGACTCGCA AGGCACGTCA AAGAGGTTCA CATTCTCAAA 
GGGAATGGAC GCATTGTCCC CCCTTCTGAG AGAAGCAAAA ATTACATGGC TTCGAAAGGT 
CCTACGATAC TACCCCCCAT GGAAGCTGAA ACAATAGCAA CAGCAACACA AACCAGCGGT 
GTTCCAGCTA CTAAACAACC GGAGCCGTTA TTTATTGCTG TGCCGCCACG ACCTAGTCGT 
GTCTTACATT CAGATGCATA TTTACGGTAT ATCGAGGGAC TGAACGTGGA TAATCGCTAC 
ATATCAAATT GGGATAAACA AATGAATGCC ACTGCAGAAA ACACGCAAAT TCCGGATCTG 
ACAAAACTTC CAGCTGAGTG GCTGGGCAAT GGCGTCGGCA ATCATGGCAA CGTCGTGAAT 
GCCTTGTGGA CTCTCAGGGA CATGATGATG CGCGATGTGT TGGCCATCAA TAAAACTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588629.1
CDS	157..5319
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733143.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733143.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGAGGC ATCGCCGTTG CTACCGAAGC TGGAGCCAGA TGTGCTCGGG 
ATGGGAGTGG GATTGGGAGT GGGAGTGGGA GTGGGTGAGG ACAGCATGGA TGGCGGTGGA 
CCACCGTCCG TCGGATCCAG CGAAGCTCAG CCGGTGCTCC AATGCTCCAA TGCTCAGACA 
CCTGTATCGA CAAAAAAGAA ATCGACGGGT AAGAAATTAG TTACGGGCTA TATTTTGTAT 
TCGAGTAAAA TGCGAACGCA GATTACGCAA AACAATCCTG AATCGTCCTT CGGAGAGATT 
AGCCGAATTG TTGGCAATGA GTGGCGGAAG TTGCCAGCTG GTGAGAAACA GGCGTGGGAG 
GAACGTGCAG TCAAGATGAA CGAAGATGGT CCGAACGGTA CTGGAGCTGG TGGTGGTGCT 
GGGGGTGGCG GAGCAGGTGG CGGTGGCGGC GCCGGAAGTG TCGCTGGTGG CGGTACCGGT 
GGCTTCTTCG GAACTGGAGG AACTGGTACT GGTGGTGGAG GCGTCGGTGG TGCCGGCGCT 
GGTGGCGGCG GCAACCAATC GACGAAAGGA ACAAGTGTTA ATGCTGCCAA CTCTTCACCA 
GATCAGGTCT ACGAATGTTG CTGGGACAAT TGTGACTGGC AATTTGAGGA CATGACGGAT 
TGCATCGATC ACTGTATTGC TGAGCAAAGT GGACACGTGC AGAACTCTTT CTTAAACGCA 
TCCAGTGACG TGGAGTTTCA GTGCCAATGG CGCGGATGCG GAAGAACAAA GAAAACTGTA 
CCTCCGTTTC CAAGTGTACA ACGACTCGCA AGGCACGTCA AAGAGGTTCA CATTCTCAAA 
GGGAATGGAC GCATTGTCCC CCCTTCTGAG AGAAGCAAAA ATTACATGGC TTCGAAAGGT 
CCTACGATAC TACCCCCCAT GGAAGCTGAA ACAATAGCAA CAGCAACACA AACCAGCGGT 
GTTCCAGCTA CTAAACAACC GGAGCCGTTA TTTATTGCTG TGCCGCCACG ACCTAGTCGT 
GTCTTACATT CAGATGCATA TTTACGGTAT ATCGAGGGAC TGAACGTGGA TAATCGCTAC 
ATATCAAATT GGGATAAACA AATGAATGCC ACTGCAGAAA ACACGCAAAT TCCGGATCTG 
ACAAAACTTC CAGCTGAGTG GCTGGGCAAT GGCGTCGGCA ATCATGGCAA CGTCGTGAAT 
GCCTTGTGGA CTCTCAGGGA CATGATGATG CGCGATGTGT TGGCCATCAA TAAAACTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733143.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588629.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.

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CloneID	OCe76240
Clone ID Related Accession (Same CDS sequence)	XM_015733143.1 , XM_015733143.2
Accession Version	XM_015733143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015733143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGAGGC ATCGCCGTTG CTACCGAAGC TGGAGCCAGA TGTGCTCGGG 
ATGGGAGTGG GATTGGGAGT GGGAGTGGGA GTGGGTGAGG ACAGCATGGA TGGCGGTGGA 
CCACCGTCCG TCGGATCCAG CGAAGCTCAG CCGGTGCTCC AATGCTCCAA TGCTCAGACA 
CCTGTATCGA CAAAAAAGAA ATCGACGGGT AAGAAATTAG TTACGGGCTA TATTTTGTAT 
TCGAGTAAAA TGCGAACGCA GATTACGCAA AACAATCCTG AATCGTCCTT CGGAGAGATT 
AGCCGAATTG TTGGCAATGA GTGGCGGAAG TTGCCAGCTG GTGAGAAACA GGCGTGGGAG 
GAACGTGCAG TCAAGATGAA CGAAGATGGT CCGAACGGTA CTGGAGCTGG TGGTGGTGCT 
GGGGGTGGCG GAGCAGGTGG CGGTGGCGGC GCCGGAAGTG TCGCTGGTGG CGGTACCGGT 
GGCTTCTTCG GAACTGGAGG AACTGGTACT GGTGGTGGAG GCGTCGGTGG TGCCGGCGCT 
GGTGGCGGCG GCAACCAATC GACGAAAGGA ACAAGTGTTA ATGCTGCCAA CTCTTCACCA 
GATCAGGTCT ACGAATGTTG CTGGGACAAT TGTGACTGGC AATTTGAGGA CATGACGGAT 
TGCATCGATC ACTGTATTGC TGAGCAAAGT GGACACGTGC AGAACTCTTT CTTAAACGCA 
TCCAGTGACG TGGAGTTTCA GTGCCAATGG CGCGGATGCG GAAGAACAAA GAAAACTGTA 
CCTCCGTTTC CAAGTGTACA ACGACTCGCA AGGCACGTCA AAGAGGTTCA CATTCTCAAA 
GGGAATGGAC GCATTGTCCC CCCTTCTGAG AGAAGCAAAA ATTACATGGC TTCGAAAGGT 
CCTACGATAC TACCCCCCAT GGAAGCTGAA ACAATAGCAA CAGCAACACA AACCAGCGGT 
GTTCCAGCTA CTAAACAACC GGAGCCGTTA TTTATTGCTG TGCCGCCACG ACCTAGTCGT 
GTCTTACATT CAGATGCATA TTTACGGTAT ATCGAGGGAC TGAACGTGGA TAATCGCTAC 
ATATCAAATT GGGATAAACA AATGAATGCC ACTGCAGAAA ACACGCAAAT TCCGGATCTG 
ACAAAACTTC CAGCTGAGTG GCTGGGCAAT GGCGTCGGCA ATCATGGCAA CGTCGTGAAT 
GCCTTGTGGA CTCTCAGGGA CATGATGATG CGCGATGTGT TGGCCATCAA TAAAACTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588629.1
CDS	175..5337
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733143.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733143.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGAGGC ATCGCCGTTG CTACCGAAGC TGGAGCCAGA TGTGCTCGGG 
ATGGGAGTGG GATTGGGAGT GGGAGTGGGA GTGGGTGAGG ACAGCATGGA TGGCGGTGGA 
CCACCGTCCG TCGGATCCAG CGAAGCTCAG CCGGTGCTCC AATGCTCCAA TGCTCAGACA 
CCTGTATCGA CAAAAAAGAA ATCGACGGGT AAGAAATTAG TTACGGGCTA TATTTTGTAT 
TCGAGTAAAA TGCGAACGCA GATTACGCAA AACAATCCTG AATCGTCCTT CGGAGAGATT 
AGCCGAATTG TTGGCAATGA GTGGCGGAAG TTGCCAGCTG GTGAGAAACA GGCGTGGGAG 
GAACGTGCAG TCAAGATGAA CGAAGATGGT CCGAACGGTA CTGGAGCTGG TGGTGGTGCT 
GGGGGTGGCG GAGCAGGTGG CGGTGGCGGC GCCGGAAGTG TCGCTGGTGG CGGTACCGGT 
GGCTTCTTCG GAACTGGAGG AACTGGTACT GGTGGTGGAG GCGTCGGTGG TGCCGGCGCT 
GGTGGCGGCG GCAACCAATC GACGAAAGGA ACAAGTGTTA ATGCTGCCAA CTCTTCACCA 
GATCAGGTCT ACGAATGTTG CTGGGACAAT TGTGACTGGC AATTTGAGGA CATGACGGAT 
TGCATCGATC ACTGTATTGC TGAGCAAAGT GGACACGTGC AGAACTCTTT CTTAAACGCA 
TCCAGTGACG TGGAGTTTCA GTGCCAATGG CGCGGATGCG GAAGAACAAA GAAAACTGTA 
CCTCCGTTTC CAAGTGTACA ACGACTCGCA AGGCACGTCA AAGAGGTTCA CATTCTCAAA 
GGGAATGGAC GCATTGTCCC CCCTTCTGAG AGAAGCAAAA ATTACATGGC TTCGAAAGGT 
CCTACGATAC TACCCCCCAT GGAAGCTGAA ACAATAGCAA CAGCAACACA AACCAGCGGT 
GTTCCAGCTA CTAAACAACC GGAGCCGTTA TTTATTGCTG TGCCGCCACG ACCTAGTCGT 
GTCTTACATT CAGATGCATA TTTACGGTAT ATCGAGGGAC TGAACGTGGA TAATCGCTAC 
ATATCAAATT GGGATAAACA AATGAATGCC ACTGCAGAAA ACACGCAAAT TCCGGATCTG 
ACAAAACTTC CAGCTGAGTG GCTGGGCAAT GGCGTCGGCA ATCATGGCAA CGTCGTGAAT 
GCCTTGTGGA CTCTCAGGGA CATGATGATG CGCGATGTGT TGGCCATCAA TAAAACTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733229.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588715.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe76241
Clone ID Related Accession (Same CDS sequence)	XM_015733229.2 , XM_015733229.1
Accession Version	XM_015733229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015733229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGATC AGGTCTACGA ATGTTGCTGG 
GACAATTGTG ACTGGCAATT TGAGGACATG ACGGATTGCA TCGATCACTG TATTGCTGAG 
CAAAGTGGAC ACGTGCAGAA CTCTTTCTTA AACGCATCCA GTGACGTGGA GTTTCAGTGC 
CAATGGCGCG GATGCGGAAG AACAAAGAAA ACTGTACCTC CGTTTCCAAG TGTACAACGA 
CTCGCAAGGC ACGTCAAAGA GGTTCACATT CTCAAAGGGA ATGGACGCAT TGTCCCCCCT 
TCTGAGAGAA GCAAAAATTA CATGGCTTCG AAAGGTCCTA CGATACTACC CCCCATGGAA 
GCTGAAACAA TAGCAACAGC AACACAAACC AGCGGTGTTC CAGCTACTAA ACAACCGGAG 
CCGTTATTTA TTGCTGTGCC GCCACGACCT AGTCGTGTCT TACATTCAGA TGCATATTTA 
CGGTATATCG AGGGACTGAA CGTGGATAAT CGCTACATAT CAAATTGGGA TAAACAAATG 
AATGCCACTG CAGAAAACAC GCAAATTCCG GATCTGACAA AACTTCCAGC TGAGTGGCTG 
GGCAATGGCG TCGGCAATCA TGGCAACGTC GTGAATGCCT TGTGGACTCT CAGGGACATG 
ATGATGCGCG ATGTGTTGGC CATCAATAAA ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588715.1
CDS	175..5313
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733229.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733229.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGATC AGGTCTACGA ATGTTGCTGG 
GACAATTGTG ACTGGCAATT TGAGGACATG ACGGATTGCA TCGATCACTG TATTGCTGAG 
CAAAGTGGAC ACGTGCAGAA CTCTTTCTTA AACGCATCCA GTGACGTGGA GTTTCAGTGC 
CAATGGCGCG GATGCGGAAG AACAAAGAAA ACTGTACCTC CGTTTCCAAG TGTACAACGA 
CTCGCAAGGC ACGTCAAAGA GGTTCACATT CTCAAAGGGA ATGGACGCAT TGTCCCCCCT 
TCTGAGAGAA GCAAAAATTA CATGGCTTCG AAAGGTCCTA CGATACTACC CCCCATGGAA 
GCTGAAACAA TAGCAACAGC AACACAAACC AGCGGTGTTC CAGCTACTAA ACAACCGGAG 
CCGTTATTTA TTGCTGTGCC GCCACGACCT AGTCGTGTCT TACATTCAGA TGCATATTTA 
CGGTATATCG AGGGACTGAA CGTGGATAAT CGCTACATAT CAAATTGGGA TAAACAAATG 
AATGCCACTG CAGAAAACAC GCAAATTCCG GATCTGACAA AACTTCCAGC TGAGTGGCTG 
GGCAATGGCG TCGGCAATCA TGGCAACGTC GTGAATGCCT TGTGGACTCT CAGGGACATG 
ATGATGCGCG ATGTGTTGGC CATCAATAAA ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733229.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588715.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.

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CloneID	OCe76241
Clone ID Related Accession (Same CDS sequence)	XM_015733229.2 , XM_015733229.1
Accession Version	XM_015733229.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGATC AGGTCTACGA ATGTTGCTGG 
GACAATTGTG ACTGGCAATT TGAGGACATG ACGGATTGCA TCGATCACTG TATTGCTGAG 
CAAAGTGGAC ACGTGCAGAA CTCTTTCTTA AACGCATCCA GTGACGTGGA GTTTCAGTGC 
CAATGGCGCG GATGCGGAAG AACAAAGAAA ACTGTACCTC CGTTTCCAAG TGTACAACGA 
CTCGCAAGGC ACGTCAAAGA GGTTCACATT CTCAAAGGGA ATGGACGCAT TGTCCCCCCT 
TCTGAGAGAA GCAAAAATTA CATGGCTTCG AAAGGTCCTA CGATACTACC CCCCATGGAA 
GCTGAAACAA TAGCAACAGC AACACAAACC AGCGGTGTTC CAGCTACTAA ACAACCGGAG 
CCGTTATTTA TTGCTGTGCC GCCACGACCT AGTCGTGTCT TACATTCAGA TGCATATTTA 
CGGTATATCG AGGGACTGAA CGTGGATAAT CGCTACATAT CAAATTGGGA TAAACAAATG 
AATGCCACTG CAGAAAACAC GCAAATTCCG GATCTGACAA AACTTCCAGC TGAGTGGCTG 
GGCAATGGCG TCGGCAATCA TGGCAACGTC GTGAATGCCT TGTGGACTCT CAGGGACATG 
ATGATGCGCG ATGTGTTGGC CATCAATAAA ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588715.1
CDS	157..5295
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKVPSDVAQTPSQAKSVVSTMAHFEMEASPLLPKLEPDVLGMGVGLGVGVGVGEDSMDGGGPPSVGSSEAQPVLQCSNAQTPVSTKKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733229.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733229.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGGTGCC TAGCGACGTC GCACAGACGC CATCCCAAGC CAAGTCGGTT 
GTATCTACTA TGGCTCACTT TGAAATGGAG GCATCGCCGT TGCTACCGAA GCTGGAGCCA 
GATGTGCTCG GGATGGGAGT GGGATTGGGA GTGGGAGTGG GAGTGGGTGA GGACAGCATG 
GATGGCGGTG GACCACCGTC CGTCGGATCC AGCGAAGCTC AGCCGGTGCT CCAATGCTCC 
AATGCTCAGA CACCTGTATC GACAAAAAAG AAATCGACGG GTAAGAAATT AGTTACGGGC 
TATATTTTGT ATTCGAGTAA AATGCGAACG CAGATTACGC AAAACAATCC TGAATCGTCC 
TTCGGAGAGA TTAGCCGAAT TGTTGGCAAT GAGTGGCGGA AGTTGCCAGC TGGTGAGAAA 
CAGGCGTGGG AGGAACGTGC AGTCAAGATG AACGAAGATG GTCCGAACGG TACTGGAGCT 
GGTGGTGGTG CTGGGGGTGG CGGAGCAGGT GGCGGTGGCG GCGCCGGAAG TGTCGCTGGT 
GGCGGTACCG GTGGCTTCTT CGGAACTGGA GGAACTGATC AGGTCTACGA ATGTTGCTGG 
GACAATTGTG ACTGGCAATT TGAGGACATG ACGGATTGCA TCGATCACTG TATTGCTGAG 
CAAAGTGGAC ACGTGCAGAA CTCTTTCTTA AACGCATCCA GTGACGTGGA GTTTCAGTGC 
CAATGGCGCG GATGCGGAAG AACAAAGAAA ACTGTACCTC CGTTTCCAAG TGTACAACGA 
CTCGCAAGGC ACGTCAAAGA GGTTCACATT CTCAAAGGGA ATGGACGCAT TGTCCCCCCT 
TCTGAGAGAA GCAAAAATTA CATGGCTTCG AAAGGTCCTA CGATACTACC CCCCATGGAA 
GCTGAAACAA TAGCAACAGC AACACAAACC AGCGGTGTTC CAGCTACTAA ACAACCGGAG 
CCGTTATTTA TTGCTGTGCC GCCACGACCT AGTCGTGTCT TACATTCAGA TGCATATTTA 
CGGTATATCG AGGGACTGAA CGTGGATAAT CGCTACATAT CAAATTGGGA TAAACAAATG 
AATGCCACTG CAGAAAACAC GCAAATTCCG GATCTGACAA AACTTCCAGC TGAGTGGCTG 
GGCAATGGCG TCGGCAATCA TGGCAACGTC GTGAATGCCT TGTGGACTCT CAGGGACATG 
ATGATGCGCG ATGTGTTGGC CATCAATAAA ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733309.2 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588795.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X7, mRNA.

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CloneID	OCe76242
Clone ID Related Accession (Same CDS sequence)	XM_015733309.2 , XM_015733309.1
Accession Version	XM_015733309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_015733309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cephus cinctus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGAAATC GACGGGTAAG AAATTAGTTA CGGGCTATAT TTTGTATTCG 
AGTAAAATGC GAACGCAGAT TACGCAAAAC AATCCTGAAT CGTCCTTCGG AGAGATTAGC 
CGAATTGTTG GCAATGAGTG GCGGAAGTTG CCAGCTGGTG AGAAACAGGC GTGGGAGGAA 
CGTGCAGTCA AGATGAACGA AGATGGTCCG AACGGTACTG GAGCTGGTGG TGGTGCTGGG 
GGTGGCGGAG CAGGTGGCGG TGGCGGCGCC GGAAGTGTCG CTGGTGGCGG TACCGGTGGC 
TTCTTCGGAA CTGGAGGAAC TGGTACTGGT GGTGGAGGCG TCGGTGGTGC CGGCGCTGGT 
GGCGGCGGCA ACCAATCGAC GAAAGGAACA AGTGTTAATG CTGCCAACTC TTCACCAGAT 
CAGGTCTACG AATGTTGCTG GGACAATTGT GACTGGCAAT TTGAGGACAT GACGGATTGC 
ATCGATCACT GTATTGCTGA GCAAAGTGGA CACGTGCAGA ACTCTTTCTT AAACGCATCC 
AGTGACGTGG AGTTTCAGTG CCAATGGCGC GGATGCGGAA GAACAAAGAA AACTGTACCT 
CCGTTTCCAA GTGTACAACG ACTCGCAAGG CACGTCAAAG AGGTTCACAT TCTCAAAGGG 
AATGGACGCA TTGTCCCCCC TTCTGAGAGA AGCAAAAATT ACATGGCTTC GAAAGGTCCT 
ACGATACTAC CCCCCATGGA AGCTGAAACA ATAGCAACAG CAACACAAAC CAGCGGTGTT 
CCAGCTACTA AACAACCGGA GCCGTTATTT ATTGCTGTGC CGCCACGACC TAGTCGTGTC 
TTACATTCAG ATGCATATTT ACGGTATATC GAGGGACTGA ACGTGGATAA TCGCTACATA 
TCAAATTGGG ATAAACAAAT GAATGCCACT GCAGAAAACA CGCAAATTCC GGATCTGACA 
AAACTTCCAG CTGAGTGGCT GGGCAATGGC GTCGGCAATC ATGGCAACGT CGTGAATGCC 
TTGTGGACTC TCAGGGACAT GATGATGCGC GATGTGTTGG CCATCAATAA AACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588795.1
CDS	175..5154
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733309.2
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733309.2

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGAAATC GACGGGTAAG AAATTAGTTA CGGGCTATAT TTTGTATTCG 
AGTAAAATGC GAACGCAGAT TACGCAAAAC AATCCTGAAT CGTCCTTCGG AGAGATTAGC 
CGAATTGTTG GCAATGAGTG GCGGAAGTTG CCAGCTGGTG AGAAACAGGC GTGGGAGGAA 
CGTGCAGTCA AGATGAACGA AGATGGTCCG AACGGTACTG GAGCTGGTGG TGGTGCTGGG 
GGTGGCGGAG CAGGTGGCGG TGGCGGCGCC GGAAGTGTCG CTGGTGGCGG TACCGGTGGC 
TTCTTCGGAA CTGGAGGAAC TGGTACTGGT GGTGGAGGCG TCGGTGGTGC CGGCGCTGGT 
GGCGGCGGCA ACCAATCGAC GAAAGGAACA AGTGTTAATG CTGCCAACTC TTCACCAGAT 
CAGGTCTACG AATGTTGCTG GGACAATTGT GACTGGCAAT TTGAGGACAT GACGGATTGC 
ATCGATCACT GTATTGCTGA GCAAAGTGGA CACGTGCAGA ACTCTTTCTT AAACGCATCC 
AGTGACGTGG AGTTTCAGTG CCAATGGCGC GGATGCGGAA GAACAAAGAA AACTGTACCT 
CCGTTTCCAA GTGTACAACG ACTCGCAAGG CACGTCAAAG AGGTTCACAT TCTCAAAGGG 
AATGGACGCA TTGTCCCCCC TTCTGAGAGA AGCAAAAATT ACATGGCTTC GAAAGGTCCT 
ACGATACTAC CCCCCATGGA AGCTGAAACA ATAGCAACAG CAACACAAAC CAGCGGTGTT 
CCAGCTACTA AACAACCGGA GCCGTTATTT ATTGCTGTGC CGCCACGACC TAGTCGTGTC 
TTACATTCAG ATGCATATTT ACGGTATATC GAGGGACTGA ACGTGGATAA TCGCTACATA 
TCAAATTGGG ATAAACAAAT GAATGCCACT GCAGAAAACA CGCAAATTCC GGATCTGACA 
AAACTTCCAG CTGAGTGGCT GGGCAATGGC GTCGGCAATC ATGGCAACGT CGTGAATGCC 
TTGTGGACTC TCAGGGACAT GATGATGCGC GATGTGTTGG CCATCAATAA AACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107264516 ( XM_015733309.1 ) cDNA ORF clone, Cephus cinctus(wheat stem sawfly) -> XP_015588795.1 Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.

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CloneID	OCe76242
Clone ID Related Accession (Same CDS sequence)	XM_015733309.2 , XM_015733309.1
Accession Version	XM_015733309.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-23
Organism	Cephus cinctus(wheat stem sawfly)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014332752.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cephus cinctus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGAAATC GACGGGTAAG AAATTAGTTA CGGGCTATAT TTTGTATTCG 
AGTAAAATGC GAACGCAGAT TACGCAAAAC AATCCTGAAT CGTCCTTCGG AGAGATTAGC 
CGAATTGTTG GCAATGAGTG GCGGAAGTTG CCAGCTGGTG AGAAACAGGC GTGGGAGGAA 
CGTGCAGTCA AGATGAACGA AGATGGTCCG AACGGTACTG GAGCTGGTGG TGGTGCTGGG 
GGTGGCGGAG CAGGTGGCGG TGGCGGCGCC GGAAGTGTCG CTGGTGGCGG TACCGGTGGC 
TTCTTCGGAA CTGGAGGAAC TGGTACTGGT GGTGGAGGCG TCGGTGGTGC CGGCGCTGGT 
GGCGGCGGCA ACCAATCGAC GAAAGGAACA AGTGTTAATG CTGCCAACTC TTCACCAGAT 
CAGGTCTACG AATGTTGCTG GGACAATTGT GACTGGCAAT TTGAGGACAT GACGGATTGC 
ATCGATCACT GTATTGCTGA GCAAAGTGGA CACGTGCAGA ACTCTTTCTT AAACGCATCC 
AGTGACGTGG AGTTTCAGTG CCAATGGCGC GGATGCGGAA GAACAAAGAA AACTGTACCT 
CCGTTTCCAA GTGTACAACG ACTCGCAAGG CACGTCAAAG AGGTTCACAT TCTCAAAGGG 
AATGGACGCA TTGTCCCCCC TTCTGAGAGA AGCAAAAATT ACATGGCTTC GAAAGGTCCT 
ACGATACTAC CCCCCATGGA AGCTGAAACA ATAGCAACAG CAACACAAAC CAGCGGTGTT 
CCAGCTACTA AACAACCGGA GCCGTTATTT ATTGCTGTGC CGCCACGACC TAGTCGTGTC 
TTACATTCAG ATGCATATTT ACGGTATATC GAGGGACTGA ACGTGGATAA TCGCTACATA 
TCAAATTGGG ATAAACAAAT GAATGCCACT GCAGAAAACA CGCAAATTCC GGATCTGACA 
AAACTTCCAG CTGAGTGGCT GGGCAATGGC GTCGGCAATC ATGGCAACGT CGTGAATGCC 
TTGTGGACTC TCAGGGACAT GATGATGCGC GATGTGTTGG CCATCAATAA AACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015588795.1
CDS	157..5136
Translation	MNKRRRTSSVASRGTEDDPEDVQPEPAKRRKKLDPSDLCQQLYDILRNQRKEDGSLLCDAFIRVPKRRQEPGYYEVVTNPIDLLKVQQKLKTDEYRDMDDLAADIQLMVKNAKAFYMRTSPEYKDATELWELCVNTKNRIMDEYEDTEPKGKIILKVGRLARKAAAKQDDAEDTSESSTNPDEESLQPYEDLFMAVMTATDPMDNNRPLHTMFQLKPSKKLYPEYYDVIETPVDLKTIARKIQEAAYNCLSDMERDLMLMCRNACQFNEPGSQIYKDAKLLKKIIGAATRRQDIGSGGNLMKTGTGAPSTRSKRGSRGMAQSLIAQTATLPDEDEESDDEEDEPTESEESDNPQWQLFQTIRTAPNNQGIRMSEYFWKLPSRRLYPDYYKMIKNPISLLQIRTKIKKGEYGTVSEVAGDMNIMFENAKKYNVHTSRLYKCAAKLQKIMQEKVQELLEFDQDSDSDSGSESNSQQPKLIKRASNLLTRGKYKDNIPLKKRLHALVKCVIEYVCEDGRQPMLMFMEKPSKKIYPDYYQVIAEPIDMLQIEANIKAEKYQSENELIQDFKLMFNNCRQYNEEGSLIYEDANTLERVLMDKVKELGPLPEIIKTIKSTASTPTRNVGRPKKVVPLHLQKLRTLYDTLKDCRDAKGRQLSLIFMKLPNKNEYPDYYEVIKQPMNMEKIASTLKNNGYETLDELVSDFVLMFDNACKYNEPDSQIYKDALILQRLVLQTKLQLSEDDESVPDVAAAVQEILATIFTALYNHQDEEGRCYSDSMAELPEHDIIDGKKVRGLSLDLIKRRLDRGAYKRLDRFQEDVFSCLERARRLSRSDSQPFEDSVELQAFFLRTRDEVTRCGDLLHSPALNYTLLDLSSQVVELKRIKLQQELSLPNEEESCDGNDSVKESATTLLTPVALPSGSTTTATAPTALSSVTIPTTGATATGVSAAGGNGVAADGGNSMSFNQEVYRAGDFAYVEPSERGMELSVVLIERLWTNAEGQEMLYGNLFYRPSETYHVASRKFLDKELFKSDAHVAVPLGKVAGRCCVLSVKDYFRMQPEGFPEKDVYVCESRYSTKARAFKKIKVWNFDPDHLKLVPRERALEPKRVISVYKERLEKHKEEIAELEEGEKLIEKERPNVIMFNPDDTENTYYEQYNTCTGTVKTGDFVYVATDGGRQQIAQIDAIWSTKDGKCYFKGPWLLTPTEIPHAPTKLFYKQELFLSTVDGTHPIVAIVGKCAVLDYGEFVCSRPTEIPEDDVFICESTYDESKNLIRKLSADGLKKFNHTSAVTDDEIYFFRRPINPAKKSTGKKLVTGYILYSSKMRTQITQNNPESSFGEISRIVGNEWRKLPAGEKQAWEERAVKMNEDGPNGTGAGGGAGGGGAGGGGGAGSVAGGGTGGFFGTGGTGTGGGGVGGAGAGGGGNQSTKGTSVNAANSSPDQVYECCWDNCDWQFEDMTDCIDHCIAEQSGHVQNSFLNASSDVEFQCQWRGCGRTKKTVPPFPSVQRLARHVKEVHILKGNGRIVPPSERSKNYMASKGPTILPPMEAETIATATQTSGVPATKQPEPLFIAVPPRPSRVLHSDAYLRYIEGLNVDNRYISNWDKQMNATAENTQIPDLTKLPAEWLGNGVGNHGNVVNALWTLRDMMMRDVLAINKTL

Target ORF information:

RefSeq Version	XM_015733309.1
Organism	Cephus cinctus(wheat stem sawfly)
Definition	Cephus cinctus protein polybromo-1 (LOC107264516), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015733309.1

ORF Insert Sequence:

ATGAACAAAC GGCGTAGGAC GTCGTCGGTG GCTAGCCGGG GCACAGAGGA TGATCCTGAA 
GATGTTCAAC CTGAACCAGC AAAAAGACGG AAAAAATTAG ATCCTAGTGA TTTATGTCAA 
CAGTTGTATG ACATACTTCG AAACCAGCGA AAAGAGGATG GAAGTCTACT GTGCGATGCG 
TTCATTCGTG TGCCCAAACG ACGACAAGAA CCAGGTTACT ATGAAGTAGT AACAAACCCC 
ATTGACCTTT TAAAAGTTCA ACAAAAATTA AAGACAGACG AGTACCGGGA CATGGATGAT 
CTAGCTGCTG ATATTCAGCT GATGGTCAAG AACGCAAAGG CCTTTTACAT GCGCACTTCA 
CCCGAGTACA AAGATGCTAC TGAACTGTGG GAACTGTGTG TGAACACAAA AAATCGTATA 
ATGGATGAAT ACGAAGACAC AGAACCTAAG GGAAAGATCA TTCTAAAGGT GGGGAGACTG 
GCACGCAAAG CGGCAGCAAA ACAAGATGAC GCGGAGGATA CATCCGAGAG TTCAACAAAT 
CCCGACGAGG AATCCCTACA GCCTTATGAA GATCTGTTCA TGGCCGTAAT GACAGCAACG 
GATCCTATGG ATAACAATAG ACCATTACAC ACCATGTTTC AGTTGAAACC ATCAAAGAAG 
TTGTATCCAG AATATTATGA TGTGATCGAG ACACCAGTGG ATCTGAAGAC CATCGCACGG 
AAGATCCAGG AAGCGGCATA CAATTGTCTT TCAGATATGG AAAGAGACTT AATGTTAATG 
TGCCGTAACG CGTGCCAGTT CAATGAGCCA GGCTCACAGA TATACAAGGA TGCCAAGCTC 
CTGAAGAAGA TAATCGGAGC AGCAACTAGG AGGCAGGATA TTGGATCTGG TGGGAATCTC 
ATGAAGACGG GAACTGGTGC ACCGTCCACG AGGAGCAAAA GAGGAAGTAG GGGTATGGCG 
CAGTCTCTGA TAGCGCAGAC AGCTACGCTT CCGGATGAGG ACGAGGAGAG TGACGATGAA 
GAGGACGAGC CGACCGAGTC AGAAGAATCT GATAATCCCC AGTGGCAACT GTTCCAAACT 
ATTCGAACCG CTCCAAATAA TCAAGGAATC CGTATGAGCG AGTACTTCTG GAAGTTGCCG 
TCAAGGCGGC TCTATCCAGA CTACTATAAG ATGATCAAGA ATCCAATATC ACTTTTGCAG 
ATTCGCACAA AGATAAAGAA GGGGGAATAC GGCACGGTTA GCGAAGTCGC TGGTGACATG 
AATATAATGT TTGAGAACGC AAAAAAGTAC AACGTTCATA CTTCCAGATT GTACAAGTGC 
GCAGCCAAGC TCCAAAAAAT AATGCAGGAG AAGGTGCAGG AACTTTTAGA ATTTGATCAG 
GATTCAGACT CGGACAGTGG TTCGGAAAGT AATTCGCAAC AACCTAAATT AATAAAACGT 
GCGTCGAATC TTTTGACTCG TGGGAAATAC AAAGACAACA TACCTCTGAA GAAGCGTTTG 
CACGCGCTGG TTAAGTGCGT CATAGAATAC GTCTGTGAAG ATGGTCGACA ACCAATGTTA 
ATGTTCATGG AGAAACCATC GAAAAAGATC TATCCAGATT ATTATCAAGT GATTGCCGAG 
CCAATTGACA TGCTACAGAT AGAAGCAAAC ATCAAGGCTG AAAAGTACCA AAGTGAAAAT 
GAACTGATAC AAGATTTCAA ATTGATGTTT AACAACTGTC GTCAGTACAA CGAGGAAGGT 
TCGTTAATTT ACGAGGATGC AAATACTTTG GAAAGAGTTT TGATGGACAA AGTGAAAGAG 
TTGGGTCCAC TGCCGGAAAT AATTAAGACA ATTAAATCGA CAGCATCGAC GCCTACAAGG 
AACGTTGGGA GACCGAAGAA AGTTGTACCT TTACATTTAC AAAAATTGCG TACCCTCTAT 
GACACTCTCA AAGATTGTCG TGATGCCAAG GGTCGTCAGT TGTCCTTAAT ATTTATGAAA 
TTACCAAATA AAAATGAGTA TCCCGATTAT TATGAGGTCA TAAAACAGCC AATGAACATG 
GAGAAAATTG CCTCCACTTT AAAGAACAAC GGATATGAAA CTTTGGATGA ACTGGTGTCA 
GACTTTGTAT TGATGTTTGA TAACGCCTGC AAATACAACG AGCCCGATTC ACAAATTTAT 
AAGGATGCCC TAATTTTGCA ACGATTGGTA CTACAAACAA AGCTACAACT GAGCGAGGAC 
GACGAGAGTG TTCCGGATGT GGCAGCTGCC GTTCAGGAAA TACTTGCAAC GATATTCACT 
GCCCTGTATA ATCATCAAGA CGAGGAGGGT CGTTGTTACT CAGACTCCAT GGCAGAACTT 
CCGGAGCACG ACATAATTGA CGGCAAGAAA GTGCGAGGTC TATCCTTGGA TCTGATAAAA 
CGAAGATTGG ACCGTGGAGC GTACAAAAGA TTAGATCGTT TCCAAGAGGA TGTCTTCTCC 
TGCCTGGAAA GAGCACGCAG GTTGTCGCGA AGCGATTCTC AGCCTTTCGA AGACAGCGTC 
GAGCTACAGG CGTTCTTTCT GCGTACTCGG GACGAGGTCA CTCGTTGCGG TGACCTTCTG 
CACTCTCCCG CTCTCAACTA CACTCTGCTT GATTTGTCCA GTCAAGTTGT TGAGCTGAAG 
CGCATAAAAC TTCAGCAGGA GTTGTCGCTG CCGAACGAGG AGGAGAGTTG CGACGGCAAC 
GACAGCGTTA AGGAGTCGGC GACAACACTT CTGACACCCG TTGCTCTACC ATCCGGAAGT 
ACAACGACAG CGACTGCTCC AACAGCACTG AGTTCCGTAA CGATACCAAC GACAGGAGCA 
ACGGCAACTG GTGTATCAGC TGCTGGCGGC AACGGTGTAG CTGCCGACGG TGGCAACTCG 
ATGAGCTTCA ACCAGGAAGT TTATAGAGCC GGGGACTTTG CTTACGTTGA GCCATCGGAA 
CGCGGCATGG AACTGAGCGT CGTTCTCATT GAGCGTCTTT GGACAAATGC AGAAGGGCAA 
GAGATGCTTT ATGGAAATTT ATTCTATAGA CCTAGTGAAA CGTATCACGT GGCCTCTAGA 
AAGTTTCTCG ACAAGGAACT CTTCAAGAGC GATGCTCACG TTGCTGTGCC TCTGGGTAAA 
GTAGCTGGTC GTTGCTGCGT CCTGAGCGTC AAGGACTACT TTAGAATGCA GCCCGAAGGA 
TTTCCCGAGA AGGATGTCTA CGTCTGCGAG TCCCGATACT CGACGAAGGC TAGAGCCTTC 
AAAAAAATAA AAGTTTGGAA CTTCGATCCG GATCATCTGA AGCTGGTGCC AAGAGAACGA 
GCCTTGGAAC CAAAACGTGT AATTTCGGTT TACAAGGAGC GACTCGAGAA GCATAAGGAA 
GAGATTGCTG AGCTGGAGGA GGGTGAAAAA CTCATCGAAA AGGAGAGACC GAATGTGATA 
ATGTTCAATC CGGACGACAC CGAGAACACG TATTACGAGC AGTACAACAC GTGCACGGGA 
ACCGTGAAAA CCGGTGACTT TGTCTACGTC GCTACCGATG GCGGAAGACA ACAAATTGCG 
CAGATCGATG CCATCTGGTC GACGAAAGAC GGAAAGTGTT ACTTCAAGGG TCCCTGGTTA 
TTGACTCCTA CGGAAATTCC TCACGCACCC ACCAAACTCT TCTACAAGCA GGAACTCTTC 
TTGTCCACGG TCGATGGTAC ACATCCGATC GTCGCCATTG TTGGAAAGTG TGCTGTATTG 
GACTACGGTG AATTTGTATG CAGTCGTCCA ACGGAAATCC CGGAAGACGA CGTATTCATA 
TGCGAGTCGA CTTACGATGA GAGCAAAAAT TTGATAAGGA AGCTCAGTGC GGATGGCTTG 
AAGAAATTTA ATCATACCTC GGCAGTTACG GACGACGAGA TTTACTTTTT CCGTCGGCCC 
ATCAATCCGG CAAAGAAATC GACGGGTAAG AAATTAGTTA CGGGCTATAT TTTGTATTCG 
AGTAAAATGC GAACGCAGAT TACGCAAAAC AATCCTGAAT CGTCCTTCGG AGAGATTAGC 
CGAATTGTTG GCAATGAGTG GCGGAAGTTG CCAGCTGGTG AGAAACAGGC GTGGGAGGAA 
CGTGCAGTCA AGATGAACGA AGATGGTCCG AACGGTACTG GAGCTGGTGG TGGTGCTGGG 
GGTGGCGGAG CAGGTGGCGG TGGCGGCGCC GGAAGTGTCG CTGGTGGCGG TACCGGTGGC 
TTCTTCGGAA CTGGAGGAAC TGGTACTGGT GGTGGAGGCG TCGGTGGTGC CGGCGCTGGT 
GGCGGCGGCA ACCAATCGAC GAAAGGAACA AGTGTTAATG CTGCCAACTC TTCACCAGAT 
CAGGTCTACG AATGTTGCTG GGACAATTGT GACTGGCAAT TTGAGGACAT GACGGATTGC 
ATCGATCACT GTATTGCTGA GCAAAGTGGA CACGTGCAGA ACTCTTTCTT AAACGCATCC 
AGTGACGTGG AGTTTCAGTG CCAATGGCGC GGATGCGGAA GAACAAAGAA AACTGTACCT 
CCGTTTCCAA GTGTACAACG ACTCGCAAGG CACGTCAAAG AGGTTCACAT TCTCAAAGGG 
AATGGACGCA TTGTCCCCCC TTCTGAGAGA AGCAAAAATT ACATGGCTTC GAAAGGTCCT 
ACGATACTAC CCCCCATGGA AGCTGAAACA ATAGCAACAG CAACACAAAC CAGCGGTGTT 
CCAGCTACTA AACAACCGGA GCCGTTATTT ATTGCTGTGC CGCCACGACC TAGTCGTGTC 
TTACATTCAG ATGCATATTT ACGGTATATC GAGGGACTGA ACGTGGATAA TCGCTACATA 
TCAAATTGGG ATAAACAAAT GAATGCCACT GCAGAAAACA CGCAAATTCC GGATCTGACA 
AAACTTCCAG CTGAGTGGCT GGGCAATGGC GTCGGCAATC ATGGCAACGT CGTGAATGCC 
TTGTGGACTC TCAGGGACAT GATGATGCGC GATGTGTTGG CCATCAATAA AACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC107264516 cDNA ORF clone, Cephus cinctus(wheat stem sawfly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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