NAE1 cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAE1
Entrez Gene ID	104169328
Full Name	NEDD8 activating enzyme E1 subunit 1
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009710226.1	XP_009708528.1	NEDD8-activating enzyme E1 regulatory subunit

Pathways from BioSystems
Gene Ontology

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa455403	XM_009710226.1 Latest version!	Cariama cristata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAE1 ( XM_009710226.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009708528.1 Cariama cristata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Price & Availability↑

CloneID	OCa455403
Clone ID Related Accession (Same CDS sequence)	XM_009710226.1
Accession Version	XM_009710226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009600933.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCAACAGC AACAGGAACC GAAATACTCA AAAACTTGGT GCTACCAGGT GTTGGTTCAT 
TTACAATTGT CGATGGGAAT CGGGTCTCTG GAGAAGATGT TGGAAATAAG TAGCTTCTTT 
CTACAAAAAA GTCATATTGG TCAGAATCGT GCCCAGAGCG CCACTGAGCT CTTGCAGGAA 
TTGAATAATG ATGTTTCTGG AAACTTTGTC GAAGAGAGTC CAGAGAAACT TCTAGACAAT 
GACCCTTCCT TTTTTAATCG GTTTAACTTA GTGGTTGCAA CACAACTACC AGAAAGTACT 
TTGCTGCGCT TGGCTGAACT TCTCTGGAAT TCTAATGTTC CTCTGCTGGT CTGCAGGACT 
TATGGACTGG TTGGTTATAT GAGAGTCATT ATTAAAGAAC ATACAGTTGT TGAATCTCAT 
CCTGACAATA CGTTAGAAGA TCTGAGACTG GACAAGCCAT TTCCAGAACT GAGGGAACAC 
ATTCAGTCTT ATGACTTGGA TCATATGGAC AAAAAGGACC ATAGCCATAC TCCATGGATT 
GTGATTGTAG CCAAGTATCT AACCAAATGG TTCAACGAGA AAAGTGAGCA GTTGCCTAAG 
AGTTACAGAG AAAAAGAAGC CTTCAGACAA CTGATTCGAC AAGGTATCTT AAAGAATGAA 
AATGGGACCC CAGAAGATGA GGAAAACTTT GAAGAAGCTA TAAAAAATGT GAACACAGCA 
TTAAATACTA CAGAGATTCC AAGGTGCATT GAAGAGATTT TTAATGATGA TTGCTGCATA 
AAACTCACAG AACAGTCACC CTCCTTCTGG ATTTTGGCTC GAGCTGTAAA GGAATTTGTG 
GCAAATGAGG GGCAAGGAAG CCTGCCTGTC CGCGGCACTA TTCCTGATAT GATAGCAGAC 
TCCAATAAAT TTATCAAATT GCAAAATGTA TACCGTGAAA AAGCGAAGAA GGATATTGCT 
GCTGTGGGTA ACCATGCTGC TAAATTGTTG CAATCCCTAG GCAAGGCACC CGAGTCTATT 
TCAGAGAGAG AATTAAAATT ACTTTGCAGC AACTCAGCTT TTCTACGAGT AGTGCGATGT 
AGGTCTCTGT CTGAAGAATA CGGCTTAAAC ACTTTTAACA AGGATGAAAT CATTTCCCAT 
ATGGATAACC CAGACAGTGA GGTGGTGCTC TACTTGATGC TACGGGCTGT AGATAGATTT 
TATAAGCAGC ATGGTAGATA TCCAGGTGTC TACAACGATC AGGTAGAAGA TGATATTGGA 
AAACTAAAAT CCTGCCTTAC TGCTTTCTTA CAAGAACATG GGTTGTCTGT AGTGGTGAAA 
GATGACTATG TTCATGAGTT TTGTCGTTAC GGAGCTGCTG AGCCACATGC TATTGCTGCC 
TTCATGGGAG GAGCTGCTGC ACAGGAGGTT ATCAAAGTCA TTACAGGGCA GTTTGTAATT 
TTTAATAACA CCTATATTTA TAGTGGAATG TCACAGACTT CAGCGACTTT CCAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009708528.1
CDS	1..1500
Translation	MQQQQEPKYSKTWCYQVLVHLQLSMGIGSLEKMLEISSFFLQKSHIGQNRAQSATELLQELNNDVSGNFVEESPEKLLDNDPSFFNRFNLVVATQLPESTLLRLAELLWNSNVPLLVCRTYGLVGYMRVIIKEHTVVESHPDNTLEDLRLDKPFPELREHIQSYDLDHMDKKDHSHTPWIVIVAKYLTKWFNEKSEQLPKSYREKEAFRQLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTEIPRCIEEIFNDDCCIKLTEQSPSFWILARAVKEFVANEGQGSLPVRGTIPDMIADSNKFIKLQNVYREKAKKDIAAVGNHAAKLLQSLGKAPESISERELKLLCSNSAFLRVVRCRSLSEEYGLNTFNKDEIISHMDNPDSEVVLYLMLRAVDRFYKQHGRYPGVYNDQVEDDIGKLKSCLTAFLQEHGLSVVVKDDYVHEFCRYGAAEPHAIAAFMGGAAAQEVIKVITGQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_009710226.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009710226.1

ORF Insert Sequence:

ATGCAACAGC AACAGGAACC GAAATACTCA AAAACTTGGT GCTACCAGGT GTTGGTTCAT 
TTACAATTGT CGATGGGAAT CGGGTCTCTG GAGAAGATGT TGGAAATAAG TAGCTTCTTT 
CTACAAAAAA GTCATATTGG TCAGAATCGT GCCCAGAGCG CCACTGAGCT CTTGCAGGAA 
TTGAATAATG ATGTTTCTGG AAACTTTGTC GAAGAGAGTC CAGAGAAACT TCTAGACAAT 
GACCCTTCCT TTTTTAATCG GTTTAACTTA GTGGTTGCAA CACAACTACC AGAAAGTACT 
TTGCTGCGCT TGGCTGAACT TCTCTGGAAT TCTAATGTTC CTCTGCTGGT CTGCAGGACT 
TATGGACTGG TTGGTTATAT GAGAGTCATT ATTAAAGAAC ATACAGTTGT TGAATCTCAT 
CCTGACAATA CGTTAGAAGA TCTGAGACTG GACAAGCCAT TTCCAGAACT GAGGGAACAC 
ATTCAGTCTT ATGACTTGGA TCATATGGAC AAAAAGGACC ATAGCCATAC TCCATGGATT 
GTGATTGTAG CCAAGTATCT AACCAAATGG TTCAACGAGA AAAGTGAGCA GTTGCCTAAG 
AGTTACAGAG AAAAAGAAGC CTTCAGACAA CTGATTCGAC AAGGTATCTT AAAGAATGAA 
AATGGGACCC CAGAAGATGA GGAAAACTTT GAAGAAGCTA TAAAAAATGT GAACACAGCA 
TTAAATACTA CAGAGATTCC AAGGTGCATT GAAGAGATTT TTAATGATGA TTGCTGCATA 
AAACTCACAG AACAGTCACC CTCCTTCTGG ATTTTGGCTC GAGCTGTAAA GGAATTTGTG 
GCAAATGAGG GGCAAGGAAG CCTGCCTGTC CGCGGCACTA TTCCTGATAT GATAGCAGAC 
TCCAATAAAT TTATCAAATT GCAAAATGTA TACCGTGAAA AAGCGAAGAA GGATATTGCT 
GCTGTGGGTA ACCATGCTGC TAAATTGTTG CAATCCCTAG GCAAGGCACC CGAGTCTATT 
TCAGAGAGAG AATTAAAATT ACTTTGCAGC AACTCAGCTT TTCTACGAGT AGTGCGATGT 
AGGTCTCTGT CTGAAGAATA CGGCTTAAAC ACTTTTAACA AGGATGAAAT CATTTCCCAT 
ATGGATAACC CAGACAGTGA GGTGGTGCTC TACTTGATGC TACGGGCTGT AGATAGATTT 
TATAAGCAGC ATGGTAGATA TCCAGGTGTC TACAACGATC AGGTAGAAGA TGATATTGGA 
AAACTAAAAT CCTGCCTTAC TGCTTTCTTA CAAGAACATG GGTTGTCTGT AGTGGTGAAA 
GATGACTATG TTCATGAGTT TTGTCGTTAC GGAGCTGCTG AGCCACATGC TATTGCTGCC 
TTCATGGGAG GAGCTGCTGC ACAGGAGGTT ATCAAAGTCA TTACAGGGCA GTTTGTAATT 
TTTAATAACA CCTATATTTA TAGTGGAATG TCACAGACTT CAGCGACTTT CCAGCTGTAG