GAD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAD2
Entrez Gene ID	487107
Full Name	glutamate decarboxylase 2
Synonyms	GAD65
General protein information	Preferred Names glutamate decarboxylase 2 Names glutamate decarboxylase 2 65 kDa glutamic acid decarboxylase GAD-65 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) glutamate decarboxylase 65 kDa isoform glutamic acid decarboxylase 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077439.1	NP_001070907.1	glutamate decarboxylase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAD2	NP_000809.1
Pan troglodytes (chimpanzee)	GAD2	XP_001157825.1
	GAD2	XP_002805636.1
Bos taurus (cattle)	GAD2	NP_001192652.1
Gallus gallus (chicken)	GAD2	XP_418596.3
Xenopus tropicalis (tropical clawed frog)	gad2	XP_002933179.1
Canis lupus familiaris (dog)	GAD2	NP_001070907.1
Mus musculus (house mouse)	Gad2	NP_032104.2
Rattus norvegicus (Norway rat)	Gad2	NP_036695.1
Danio rerio (zebrafish)	gad2	NP_001017708.2

Gene Ontology
Bibliography

Related articles in PubMed

[GAT-1 and GAD65 mRNA expressions in different brain regions in dogs at brain propofol uptake equilibrium].
Yang J, Lin C, Gu M, Li Y, Liu Y, Fan Q, Chen Y
Nan fang yi ke da xue xue bao = Journal of Southern Medical University32(10)1469-73(2012 Oct)

Comparison of GAD65 and 67 immunoreactivity in the lumbar spinal cord between young adult and aged dogs.
Lee HJ, Choi JH, Ahn JH, Lee CH, Yoo KY, Hwang IK, Kim JS, Kim C, Lee YL, Shin HC, Won MH
Neurochemical research36(3)435-42(2011 Mar)

Autoantibodies to GAD65 and IA-2 in canine diabetes mellitus.
Davison LJ, Weenink SM, Christie MR, Herrtage ME, Catchpole B
Veterinary immunology and immunopathology126(1-2)83-90(2008 Nov)

Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds.
Arata S, Hashizume C, Kikusui T, Takeuchi Y, Mori Y
The Journal of veterinary medical science70(10)1107-10(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GAT-1 mRNA in the hypothalamus and hippocampus and GAD65 mRNA in the dorsal thalamus are upregulated when propofol uptake reaches an equilibrium in the brain of dogs.
Title: [GAT-1 and GAD65 mRNA expressions in different brain regions in dogs at brain propofol uptake equilibrium].

Results indicate that the distribution of GAD65-immunoreactive structures is different from GAD67-immunoreactive structures and that their immunoreactivity in the aged dogs is much higher than the adult dogs.
Title: Comparison of GAD65 and 67 immunoreactivity in the lumbar spinal cord between young adult and aged dogs.

These data indicate that serological reactivity to GAD65 and IA-2 is present in a proportion of diabetic dogs and suggest that, in some cases, canine diabetes is associated with an autoimmune response to these antigens.
Title: Autoantibodies to GAD65 and IA-2 in canine diabetes mellitus.

The following GAD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33884	NM_001077439.1 Latest version!	Canis lupus familiaris glutamate decarboxylase 2 (GAD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAD2 ( NM_001077439.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001070907.1 Canis lupus familiaris glutamate decarboxylase 2 (GAD2), mRNA.

Price & Availability↑

CloneID	OCe33884
Clone ID Related Accession (Same CDS sequence)	NM_001077439.1
Accession Version	NM_001077439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	glutamate decarboxylase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ060442.1. On Oct 31, 2006 this sequence version replaced XM_544235.2. ##Evidence-Data-START## Transcript exon combination :: AB261623.1, DQ060442.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGCATCTC CAGGCTCTGG CTTCTGGTCC TTCGGGTCTG AAGATGGCTC CGGGGATCCC 
GAGAACCCCA GCACAGCGAG AGCCTGGTGT CAGGTGGCCC AGAAGTTCAC GGGCGGCATC 
GGAAACAAGC TGTGCGCCCT GCTCTACGGA GATGCCGAGA AGCCCGCGGA GAGTGGCGGG 
AGCGAGCCCC CGCGCGCCAC CTCCAGGAAG GCCGCCTGCG CTTGTAATCA GAAGCCTTGC 
AGCTGCCCCA AAGCGGAGGT CAACTATGCG TTTCTACACG CAACAGACCT GCTGCCAGCC 
TGTGATGGAG AAAGGCCCAC GTTGGCGTTT CTGCAAGATG TTATGGACAT TTTGCTTCAG 
TATGTTGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA CCCTAATGAG 
CTCCTTCAAG AGTATAACTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACGACTCT AAAATATGCA ATTAAAACAG GGCATCCCAG ATATTTCAAT 
CAGCTTTCCA CTGGACTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AACACAAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTCTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCGGGAGGCT CTGGCGATGG GATATTTTCT 
CCTGGTGGCG CTATTTCTAA CATGTATGCC ATGCTGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCGGTT CCCAGGCTCA TTGCCTTCAC ATCTGAGCAT 
AGTCACTTTT CTCTCAAGAA GGGAGCTGCA GCTTTGGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG GGGGAAAATG GTCCCATCTG ATCTTGAAAG AAGGATCCTT 
GAAGCCAAAC AAAAAGGATT TGTTCCTTTC CTTGTGAGCG CCACAGCTGG GACCACCGTG 
TATGGAGCAT TCGACCCCCT CTTAGCAGTT GCTGACATTT GTAAAAAGTA CAAGATCTGG 
ATGCATGTGG ATGCTGCTTG GGGTGGGGGA TTACTGATGT CCCGGAAGCA CAAATGGAAG 
CTGAGCGGCG TGGAGAGGGC CAACTCTGTG ACATGGAACC CACACAAGAT GATGGGCGTC 
CCTTTACAGT GCTCCGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAG TTGCAACCAG 
ATGCATGCCT CCTACCTCTT CCAGCAAGAT AAACACTATG ACCTGTCCTA TGATACTGGG 
GATAAGGCCT TACAGTGTGG ACGCCACGTT GATGTTTTTA AATTATGGCT AATGTGGAGG 
GCAAAGGGCA CCACTGGGTT TGAAGCACAT ATTGATAAGT GCCTGGAGCT GGCTGAGTAT 
TTATACAGTA TCATAAAAAA CCGAGAAGGA TACGAAATGG TGTTTGATGG AAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACGTGCCT CCAAGTTTGC GTGTCCTGGA AGACAATGAA 
GAGAGAATGA ACCGCCTCTC AAAGGTGGCC CCAGTGATTA AAGCCCGAAT GATGGAGTAT 
GGGACCACAA TGGTCAGCTA TCAGCCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTT 
ATCTCAAATC CCGCAGCAAC TCACCAAGAC ATCGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070907.1
CDS	1..1758
Translation	MASPGSGFWSFGSEDGSGDPENPSTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSEPPRATSRKAACACNQKPCSCPKAEVNYAFLHATDLLPACDGERPTLAFLQDVMDILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARFKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMVPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYSIIKNREGYEMVFDGKPQHTNVCFWYVPPSLRVLEDNEERMNRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_001077439.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glutamate decarboxylase 2 (GAD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077439.1

ORF Insert Sequence:

ATGGCATCTC CAGGCTCTGG CTTCTGGTCC TTCGGGTCTG AAGATGGCTC CGGGGATCCC 
GAGAACCCCA GCACAGCGAG AGCCTGGTGT CAGGTGGCCC AGAAGTTCAC GGGCGGCATC 
GGAAACAAGC TGTGCGCCCT GCTCTACGGA GATGCCGAGA AGCCCGCGGA GAGTGGCGGG 
AGCGAGCCCC CGCGCGCCAC CTCCAGGAAG GCCGCCTGCG CTTGTAATCA GAAGCCTTGC 
AGCTGCCCCA AAGCGGAGGT CAACTATGCG TTTCTACACG CAACAGACCT GCTGCCAGCC 
TGTGATGGAG AAAGGCCCAC GTTGGCGTTT CTGCAAGATG TTATGGACAT TTTGCTTCAG 
TATGTTGTGA AAAGTTTCGA TAGATCAACC AAAGTGATTG ATTTCCATTA CCCTAATGAG 
CTCCTTCAAG AGTATAACTG GGAATTGGCA GACCAACCAC AAAATTTGGA GGAAATTTTG 
ATGCATTGCC AAACGACTCT AAAATATGCA ATTAAAACAG GGCATCCCAG ATATTTCAAT 
CAGCTTTCCA CTGGACTGGA TATGGTTGGA TTAGCAGCAG ACTGGCTGAC ATCAACAGCA 
AACACAAACA TGTTCACCTA TGAAATTGCT CCAGTATTTG TGCTCTTGGA ATATGTCACA 
CTAAAGAAAA TGAGAGAAAT CATTGGCTGG CCGGGAGGCT CTGGCGATGG GATATTTTCT 
CCTGGTGGCG CTATTTCTAA CATGTATGCC ATGCTGATCG CACGCTTTAA GATGTTCCCA 
GAAGTCAAGG AGAAAGGAAT GGCTGCGGTT CCCAGGCTCA TTGCCTTCAC ATCTGAGCAT 
AGTCACTTTT CTCTCAAGAA GGGAGCTGCA GCTTTGGGGA TTGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG GGGGAAAATG GTCCCATCTG ATCTTGAAAG AAGGATCCTT 
GAAGCCAAAC AAAAAGGATT TGTTCCTTTC CTTGTGAGCG CCACAGCTGG GACCACCGTG 
TATGGAGCAT TCGACCCCCT CTTAGCAGTT GCTGACATTT GTAAAAAGTA CAAGATCTGG 
ATGCATGTGG ATGCTGCTTG GGGTGGGGGA TTACTGATGT CCCGGAAGCA CAAATGGAAG 
CTGAGCGGCG TGGAGAGGGC CAACTCTGTG ACATGGAACC CACACAAGAT GATGGGCGTC 
CCTTTACAGT GCTCCGCTCT CCTGGTTAGA GAAGAGGGAT TGATGCAGAG TTGCAACCAG 
ATGCATGCCT CCTACCTCTT CCAGCAAGAT AAACACTATG ACCTGTCCTA TGATACTGGG 
GATAAGGCCT TACAGTGTGG ACGCCACGTT GATGTTTTTA AATTATGGCT AATGTGGAGG 
GCAAAGGGCA CCACTGGGTT TGAAGCACAT ATTGATAAGT GCCTGGAGCT GGCTGAGTAT 
TTATACAGTA TCATAAAAAA CCGAGAAGGA TACGAAATGG TGTTTGATGG AAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTACGTGCCT CCAAGTTTGC GTGTCCTGGA AGACAATGAA 
GAGAGAATGA ACCGCCTCTC AAAGGTGGCC CCAGTGATTA AAGCCCGAAT GATGGAGTAT 
GGGACCACAA TGGTCAGCTA TCAGCCCTTG GGAGACAAGG TCAATTTCTT CCGCATGGTT 
ATCTCAAATC CCGCAGCAAC TCACCAAGAC ATCGACTTCC TGATTGAAGA AATAGAACGC 
CTTGGACAAG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[GAT-1 and GAD65 mRNA expressions in different brain regions in dogs at brain propofol uptake equilibrium].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University32(10)1469-73(2012 Oct)
Yang J,Lin C,Gu M,Li Y,Liu Y,Fan Q,Chen Y

Comparison of GAD65 and 67 immunoreactivity in the lumbar spinal cord between young adult and aged dogs.
Neurochemical research36(3)435-42(2011 Mar)
Lee HJ,Choi JH,Ahn JH,Lee CH,Yoo KY,Hwang IK,Kim JS,Kim C,Lee YL,Shin HC,Won MH

Autoantibodies to GAD65 and IA-2 in canine diabetes mellitus.
Veterinary immunology and immunopathology126(1-2)83-90(2008 Nov)
Davison LJ,Weenink SM,Christie MR,Herrtage ME,Catchpole B

Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds.
The Journal of veterinary medical science70(10)1107-10(2008 Oct)
Arata S,Hashizume C,Kikusui T,Takeuchi Y,Mori Y