ECEL1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECEL1
Entrez Gene ID	486161
Full Name	endothelin converting enzyme like 1
General protein information	Preferred Names endothelin converting enzyme like 1 Names endothelin-converting enzyme-like 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014107619.2	XP_013963094.1	endothelin-converting enzyme-like 1 isoform X2
XM_014107619.1	XP_013963094.1	endothelin-converting enzyme-like 1 isoform X2
XM_543287.5	XP_543287.2	endothelin-converting enzyme-like 1 isoform X1
XM_543287.4	XP_543287.2	endothelin-converting enzyme-like 1 isoform X1

Pathways from BioSystems

Homologies

	ECEL1	XP_001117070.2
Rattus norvegicus (Norway rat)	Ecel1	NP_068544.1
Mus musculus (house mouse)	Ecel1	NP_001264854.1
Gallus gallus (chicken)	ECEL1	XP_422744.3
Pan troglodytes (chimpanzee)	ECEL1	XP_516151.3
Bos taurus (cattle)	ECEL1	XP_005203103.1
Xenopus tropicalis (tropical clawed frog)	ecel1	XP_002937386.2
Homo sapiens (human)	ECEL1	NP_004817.2
Canis lupus familiaris (dog)	ECEL1	XP_543287.2

Gene Ontology

The following ECEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe25818	XM_543287.5 Latest version!	Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe43124	XM_014107619.2 Latest version!	Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe43124	XM_014107619.1	Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe25818	XM_543287.4	Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECEL1 ( XM_543287.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_543287.2 Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe25818
Clone ID Related Accession (Same CDS sequence)	XM_543287.5 , XM_543287.4
Accession Version	XM_543287.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	endothelin-converting enzyme-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543287.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT GCCTATCAGA AGTGGGTGCG GGAGCACGGC 
CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC AGCTCTTCTT CATTGCCTTT 
GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT ACCTGCAGGT GCTGACCGAC 
AAGCATGCAC CTGAGCACTA CAGGGTGCTG GGCAGCGTGT CCCAGTTCGA GGAGTTTGGC 
CGGGCCTTCC ATTGCCCCAA GGACTCGCCC ATGAACCCTG CCCACAAGTG CTCTGTGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543287.2
CDS	103..2445
Translation	METPYSMTAHYDEFQEVKYVSRCGAGGARGASLPPGFPRGAGRGASGARAGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGPAGGGGCSEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGGGGGGGGERPGAAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKILAAYRVFMERLLSLLGADAVEQKAQEILQLEQRLANITVSEYDDLRRDVSSMYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEDEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAREMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKFILGRRLEELDWMDAETKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVHLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPLHRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	XM_543287.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543287.5

ORF Insert Sequence:

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT GCCTATCAGA AGTGGGTGCG GGAGCACGGC 
CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC AGCTCTTCTT CATTGCCTTT 
GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT ACCTGCAGGT GCTGACCGAC 
AAGCATGCAC CTGAGCACTA CAGGGTGCTG GGCAGCGTGT CCCAGTTCGA GGAGTTTGGC 
CGGGCCTTCC ATTGCCCCAA GGACTCGCCC ATGAACCCTG CCCACAAGTG CTCTGTGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( XM_543287.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_543287.2 Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe25818
Clone ID Related Accession (Same CDS sequence)	XM_543287.5 , XM_543287.4
Accession Version	XM_543287.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	endothelin-converting enzyme-like 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543287.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT GCCTATCAGA AGTGGGTGCG GGAGCACGGC 
CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC AGCTCTTCTT CATTGCCTTT 
GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT ACCTGCAGGT GCTGACCGAC 
AAGCATGCAC CTGAGCACTA CAGGGTGCTG GGCAGCGTGT CCCAGTTCGA GGAGTTTGGC 
CGGGCCTTCC ATTGCCCCAA GGACTCGCCC ATGAACCCTG CCCACAAGTG CTCTGTGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543287.2
CDS	1..2343
Translation	METPYSMTAHYDEFQEVKYVSRCGAGGARGASLPPGFPRGAGRGASGARAGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGPAGGGGCSEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGGGGGGGGERPGAAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKILAAYRVFMERLLSLLGADAVEQKAQEILQLEQRLANITVSEYDDLRRDVSSMYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEDEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAREMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKFILGRRLEELDWMDAETKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVHLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPLHRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	XM_543287.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543287.4

ORF Insert Sequence:

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT GCCTATCAGA AGTGGGTGCG GGAGCACGGC 
CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC AGCTCTTCTT CATTGCCTTT 
GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT ACCTGCAGGT GCTGACCGAC 
AAGCATGCAC CTGAGCACTA CAGGGTGCTG GGCAGCGTGT CCCAGTTCGA GGAGTTTGGC 
CGGGCCTTCC ATTGCCCCAA GGACTCGCCC ATGAACCCTG CCCACAAGTG CTCTGTGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( XM_014107619.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013963094.1 Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X2, mRNA.

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CloneID	OCe43124
Clone ID Related Accession (Same CDS sequence)	XM_014107619.2 , XM_014107619.1
Accession Version	XM_014107619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	endothelin-converting enzyme-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT CTCCACACTG TCACCCCCAG GCCTATCAGA 
AGTGGGTGCG GGAGCACGGC CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC 
AGCTCTTCTT CATTGCCTTT GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT 
ACCTGCAGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963094.1
CDS	1..2235
Translation	METPYSMTAHYDEFQEVKYVSRCGAGGARGASLPPGFPRGAGRGASGARAGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGPAGGGGCSEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGGGGGGGGERPGAAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKILAAYRVFMERLLSLLGADAVEQKAQEILQLEQRLANITVSEYDDLRRDVSSMYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEDEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAREMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKFILGRRLEELDWMDAETKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVHLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYLHTVTPRPIRSGCGSTAQSTRCTGSSTRTTSSSSLPLPRTGASSGGHSPSTCRC

Target ORF information:

RefSeq Version	XM_014107619.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris endothelin converting enzyme like 1 (ECEL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107619.2

ORF Insert Sequence:

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT CTCCACACTG TCACCCCCAG GCCTATCAGA 
AGTGGGTGCG GGAGCACGGC CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC 
AGCTCTTCTT CATTGCCTTT GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT 
ACCTGCAGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( XM_014107619.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013963094.1 Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X2, mRNA.

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CloneID	OCe43124
Clone ID Related Accession (Same CDS sequence)	XM_014107619.2 , XM_014107619.1
Accession Version	XM_014107619.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	endothelin-converting enzyme-like 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT CTCCACACTG TCACCCCCAG GCCTATCAGA 
AGTGGGTGCG GGAGCACGGC CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC 
AGCTCTTCTT CATTGCCTTT GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT 
ACCTGCAGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963094.1
CDS	1..2235
Translation	METPYSMTAHYDEFQEVKYVSRCGAGGARGASLPPGFPRGAGRGASGARAGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGPAGGGGCSEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGGGGGGGGERPGAAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKILAAYRVFMERLLSLLGADAVEQKAQEILQLEQRLANITVSEYDDLRRDVSSMYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEDEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAREMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKFILGRRLEELDWMDAETKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVHLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYLHTVTPRPIRSGCGSTAQSTRCTGSSTRTTSSSSLPLPRTGASSGGHSPSTCRC

Target ORF information:

RefSeq Version	XM_014107619.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris endothelin converting enzyme-like 1 (ECEL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107619.1

ORF Insert Sequence:

ATGGAGACCC CGTACTCGAT GACCGCGCAC TACGACGAGT TCCAGGAGGT CAAATACGTG 
AGCCGGTGCG GCGCGGGGGG CGCGCGCGGG GCCTCGCTGC CGCCCGGCTT CCCGCGGGGC 
GCGGGGCGCG GCGCCTCCGG GGCCCGGGCG GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATCCTGGC CGCTATGCTG 
GCGCTCAAGT ACCTGGGCCC GGGCCCGGCG GGCGGCGGCG GCTGCTCCGA GGGCTGCCCC 
GAGCGCAAGG CCTTCGCGCG CGCCGCCCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATA 
GACCCGTGCC AGGACTTCTA CTCCTTCGCG TGCGGCGGCT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA CGGCACCATC GCGGCCATCG GGGAGCAGAA CGAGGAGCGG 
CTGCGGCGCC TGCTGGCGCG GCCCGGGGGC GGGCCCGGCG GGGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGGCTCGG CCCGCGGCCC 
ATGCTCGAAG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGGCGG CGGCGGCGGC 
GGCGGCGAGC GCCCGGGGGC CGCGGCGCGC TGGGACCTCA ACCGGCTGCT CTACAAGGCC 
CAGGGCGTGT ACAGCGCCGC CGCGCTCTTC TCGCTCACCG TCAGCCTGGA CGACAGGAAC 
TCCTCGCGCT ACGTCATCCG CATTGACCAG GATGGGCTCA CTCTGCCGGA GAGGACCCTG 
TACTTAGCTC AGGATGAGGA GAGTGAGAAG ATCCTGGCAG CGTACCGGGT GTTCATGGAG 
CGCCTGCTCA GCCTCCTGGG AGCCGATGCC GTGGAGCAGA AGGCGCAGGA GATCCTGCAG 
CTGGAGCAGC GGCTGGCCAA CATCACAGTG TCAGAGTACG ACGACCTCCG GCGAGATGTC 
AGCTCCATGT ACAACAAGGT GACGCTGGGG CAGTTGCAGA AGATCACCCC CCATCTGCAG 
TGGAAGTGGC TGCTGGACCA GATCTTCCAG GAGGACTTCT CAGAGGATGA GGAGGTGGTG 
CTGTTGGCCA CAGACTACAT GCAGCAGGTG TCCCAGCTCA TCCGCTCCAC ACCCCGCAGG 
ATCCTGCACA ACTACCTGGT GTGGCGTGTA GTGGTGGTCC TGAGTGAGCA CCTGTCACCG 
CCATTCCGAG AAGCACTGCA CGAGCTGGCC CGTGAGATGG AGGGCAGTGA CAAGCCACAG 
GAGCTGGCCC GTGTCTGCCT GGGCCAGGCC AACCGCCACT TTGGCATGGC ACTTGGAGCT 
CTCTTTGTAC ATGAGCACTT CTCAGCTGCC AGCAAGGCCA AGGTACAGCA GCTTGTGGAA 
GACATCAAGT TCATCTTGGG CCGGCGCCTG GAGGAGCTGG ACTGGATGGA TGCCGAGACC 
AAGGCAGCTG CTCGGGCCAA GCTCCAGTAC ATGATGGTGA TGGTGGGCTA CCCGGACTTC 
CTGCTCAAAC CCGAGGCTGT GGACAAGGAG TACGAGTTCG AGGTCCACGA GAAGACCTAC 
TTCAAGAACA TCTTGAACAG CATCCGCTTC AGCATCCAGC TCTCAGTCAA GAAAATCCGG 
CAGGAGGTGG ACAAGTCCAC GTGGCTGCTC CCACCGCAGG CCCTCAATGC CTACTACCTA 
CCCAACAAGA ACCAGATGGT GTTCCCCGCA GGCATTCTGC AGCCCACGCT CTACGACCCG 
GACTTCCCGC AGTCTCTGAA CTACGGGGGC ATTGGCACCA TCATCGGACA CGAGCTGACC 
CATGGCTATG ATGACTGGGG GGGCCAGTAC GACCGCTCCG GCAATCTGTT GCACTGGTGG 
ACGGAGGCTT CCTACAGCCG CTTCCTGCGC AAGGCGGAGT GCATCGTCCA CCTGTACGAC 
AATTTCACTG TCTACAACCA GCGGGTGAAC GGAAAGCACA CCCTTGGTGA GAACATCGCG 
GACATGGGTG GCCTCAAGCT GGCCTATTAT CTCCACACTG TCACCCCCAG GCCTATCAGA 
AGTGGGTGCG GGAGCACGGC CCAGAGCACC CGCTGCACCG GCTCAAGTAC ACGCACAACC 
AGCTCTTCTT CATTGCCTTT GCCCAGAACT GGTGCATCAA GCGGCGGTCA CAGTCCATCT 
ACCTGCAGGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ECEL1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム