Ecel1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ecel1
Entrez Gene ID	60417
Full Name	endothelin converting enzyme-like 1
Synonyms	Dine
General protein information	Preferred Names endothelin converting enzyme-like 1 Names endothelin-converting enzyme-like 1 damage-induced neuronal endopeptidase metallopeptidase xce protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35
Summary	damage-induced neuronal metallo-endopeptidase; involved in neuroprotection [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596616.1	XP_017452105.1	endothelin-converting enzyme-like 1 isoform X1
XM_017596615.1	XP_017452104.1	endothelin-converting enzyme-like 1 isoform X1
NM_021776.1	NP_068544.1	endothelin-converting enzyme-like 1

Pathways from BioSystems

Homologies

	ECEL1	XP_001117070.2
Rattus norvegicus (Norway rat)	Ecel1	NP_068544.1
Mus musculus (house mouse)	Ecel1	NP_001264854.1
Gallus gallus (chicken)	ECEL1	XP_422744.3
Pan troglodytes (chimpanzee)	ECEL1	XP_516151.3
Bos taurus (cattle)	ECEL1	XP_005203103.1
Xenopus tropicalis (tropical clawed frog)	ecel1	XP_002937386.2
Homo sapiens (human)	ECEL1	NP_004817.2
Canis lupus familiaris (dog)	ECEL1	XP_543287.2

Gene Ontology
Bibliography

Related articles in PubMed

Neuronal injury-inducible gene is synergistically regulated by ATF3, c-Jun, and STAT3 through the interaction with Sp1 in damaged neurons.
Kiryu-Seo S, Kato R, Ogawa T, Nakagomi S, Nagata K, Kiyama H
The Journal of biological chemistry283(11)6988-96(2008 Mar)

Localization and ontogeny of damage-induced neuronal endopeptidase mRNA-expressing neurons in the rat nervous system.
Nagata K, Kiryu-Seo S, Kiyama H
Neuroscience141(1)299-310(2006 Aug)

Damage-induced neuronal endopeptidase (DINE/ECEL) expression is regulated by leukemia inhibitory factor and deprivation of nerve growth factor in rat sensory ganglia after nerve injury.
Kato R, Kiryu-Seo S, Kiyama H
The Journal of neuroscience : the official journal of the Society for Neuroscience22(21)9410-8(2002 Nov)

Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengers.
Kiryu-Seo S, Sasaki M, Yokohama H, Nakagomi S, Hirayama T, Aoki S, Wada K, Kiyama H
Proceedings of the National Academy of Sciences of the United States of America97(8)4345-50(2000 Apr)

Neonatal lethality in mice deficient in XCE, a novel member of the endothelin-converting enzyme and neutral endopeptidase family.
Schweizer A, Valdenaire O, K?ster A, Lang Y, Schmitt G, Lenz B, Bluethmann H, Rohrer J
The Journal of biological chemistry274(29)20450-6(1999 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sp1 recruits ATF3, c-Jun, and STAT3 to obtain the requisite synergistic effect in neuronal injury through DINE neuronal injury-inducible gene
Title: Neuronal injury-inducible gene is synergistically regulated by ATF3, c-Jun, and STAT3 through the interaction with Sp1 in damaged neurons.

Damage-induced neuronal endopeptidase play an important role in embryonic neural development, in particular in peripheral ganglia derived from the neural crest, and in some neurons from the basal plate such as the hypothalamus and cranial motor neurons.
Title: Localization and ontogeny of damage-induced neuronal endopeptidase mRNA-expressing neurons in the rat nervous system.

DINE gene expression is regulated separately by both LIF and NGF deprivation, and this regulation pattern is similar to that of galanin gene expression after nerve injury
Title: Damage-induced neuronal endopeptidase (DINE/ECEL) expression is regulated by leukemia inhibitory factor and deprivation of nerve growth factor in rat sensory ganglia after nerve injury.

The following Ecel1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ecel1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa32552	XM_017596615.1 Latest version!	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa32552	XM_017596616.1 Latest version!	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa42699	NM_021776.1 Latest version!	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ecel1 ( XM_017596616.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452105.1 Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32552
Clone ID Related Accession (Same CDS sequence)	XM_017596616.1 , XM_017596615.1
Accession Version	XM_017596616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	endothelin-converting enzyme-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGTG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGCCTCGAC ATGCGCGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGAGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACTTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CCTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452105.1
CDS	625..2952
Translation	MEAPYSMTAHYDEFQEVKYVSRCGTGGARGTSLPPGFPRSSGRSASGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGAAGTGGACPEGCPERKAFARAARFLSANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPTGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAADRPGAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKVLAAYKVFMERLLRLLGADAVEQKAQEILQLEQRLANISVSEYDDLRRDVSSVYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAKEMEGNDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAQTKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLHKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPLHRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPVHKCSVW

Target ORF information:

RefSeq Version	XM_017596616.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596616.1

ORF Insert Sequence:

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGTG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGCCTCGAC ATGCGCGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGAGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACTTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CCTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecel1 ( XM_017596615.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452104.1 Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32552
Clone ID Related Accession (Same CDS sequence)	XM_017596616.1 , XM_017596615.1
Accession Version	XM_017596615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	endothelin-converting enzyme-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGTG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGCCTCGAC ATGCGCGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGAGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACTTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CCTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452104.1
CDS	214..2541
Translation	MEAPYSMTAHYDEFQEVKYVSRCGTGGARGTSLPPGFPRSSGRSASGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGAAGTGGACPEGCPERKAFARAARFLSANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPTGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAADRPGAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEESEKVLAAYKVFMERLLRLLGADAVEQKAQEILQLEQRLANISVSEYDDLRRDVSSVYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAKEMEGNDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAQTKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLHKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPLHRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPVHKCSVW

Target ORF information:

RefSeq Version	XM_017596615.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596615.1

ORF Insert Sequence:

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGTG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGCCTCGAC ATGCGCGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGAGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACTTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CCTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ecel1 ( NM_021776.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068544.1 Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), mRNA.

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CloneID	ORa42699
Clone ID Related Accession (Same CDS sequence)	NM_021776.1
Accession Version	NM_021776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Rattus norvegicus(Norway rat)
Product	endothelin-converting enzyme-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB026293.1. ##Evidence-Data-START## Transcript exon combination :: AB026293.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGAG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGTCTCGAC ATGCGTGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGGGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACCTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CTTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068544.1
CDS	103..2430
Translation	MEAPYSMTAHYDEFQEVKYESRCGTGGARGTSLPPGFPRSSGRSASGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPGAAGTGGACPEGCPERKAFARAARFLSANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPTGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAADRPGAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEGSEKVLAAYKVFMERLLRLLGADAVEQKAQEILQLEQRLANISVSEYDDLRRDVSSVYNKVTLGQLQKITPHLQWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSPPFREALHELAKEMEGNDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAQTKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYLKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLHKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPLHRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPVHKCSVW

Target ORF information:

RefSeq Version	NM_021776.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endothelin converting enzyme-like 1 (Ecel1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021776.1

ORF Insert Sequence:

ATGGAGGCCC CGTATTCCAT GACAGCGCAC TATGATGAGT TCCAGGAAGT CAAGTACGAG 
AGCCGGTGTG GCACCGGGGG CGCCCGAGGG ACCTCCCTAC CTCCAGGCTT CCCGAGGAGC 
TCAGGGCGCA GTGCTAGCGG GGCCCGGTCG GGGCTACCGC GCTGGAACCG ACGGGAGGTG 
TGCCTGTTGT CCGGGCTGGT GTTCGCCGCC GGCCTCTGTG CCATCTTGGC AGCCATGCTG 
GCGCTCAAAT ACCTGGGCCC TGGCGCGGCG GGCACTGGCG GAGCCTGTCC CGAGGGCTGC 
CCCGAGCGCA AGGCCTTCGC GCGCGCCGCC CGCTTCCTGT CTGCCAACCT GGACGCCAGC 
ATCGACCCGT GCCAGGACTT CTACTCGTTC GCGTGCGGGG GCTGGCTGCG GCGCCACGCC 
ATCCCCGACG ACAAGCTCAC CTATGGCACG ATCGCGGCCA TCGGAGAGCA GAACGAGGAG 
CGCCTGCGGC GCCTGCTGGC GAGACCCACG GGAGGCCCGG GCGGCGCTGC GCAGCGCAAG 
GTGCGCGCCT TCTTCCGCTC GTGTCTCGAC ATGCGTGAGA TCGAGAGGCT CGGGCCGCGG 
CCCATGCTGG AGGTCATCGA GGACTGCGGC GGCTGGGACC TAGGCGGCGC GGCCGATCGC 
CCGGGGGCGG CTCGCTGGGA CCTCAACCGA CTGTTGTACA AGGCACAGGG TGTGTACAGC 
GCGGCCGCGC TCTTCTCGCT TACAGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGTATTG ACCAGGACGG GCTCACCCTG CCAGAGAGAA CCCTGTACCT AGCTCAAGAT 
GAGGGGAGTG AGAAGGTCCT GGCCGCATAC AAGGTGTTCA TGGAGCGCCT ACTCAGACTA 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCT CAGGAAATCC TTCAACTGGA GCAGAGGCTA 
GCTAATATCT CTGTGTCAGA ATATGATGAT CTCCGTCGAG ATGTCAGCTC CGTGTACAAC 
AAAGTGACTC TGGGGCAGCT GCAGAAGATC ACCCCCCATT TGCAGTGGAA ATGGCTGCTG 
GACCAGATCT TCCAGGAGGA CTTCTCTGAA GAGGAGGAGG TGGTGCTGCT GGCCACAGAC 
TACATGCAGC AGGTGTCCCA GCTCATCCGA TCCACACCCC GAAGGATCCT TCACAACTAC 
CTAGTGTGGC GTGTGGTGGT GGTTCTGAGT GAGCACCTGT CCCCACCATT CCGAGAGGCA 
CTGCATGAGC TGGCCAAAGA GATGGAGGGC AATGACAAAC CCCAGGAGTT GGCCCGAGTC 
TGCCTGGGCC AGGCCAACCG CCACTTCGGC ATGGCTCTTG GTGCCCTCTT TGTACACGAA 
CACTTCTCAG CTGCCAGTAA AGCCAAGGTA CAGCAGCTGG TGGAAGATAT CAAGTACATC 
TTAGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCC AGACCAAGGC AGCTGCTCGG 
GCTAAGCTCC AGTACATGAT GGTCATGGTT GGCTATCCAG ACTTCCTGCT GAAACCTGAG 
GCTGTAGACA AAGAGTATGA GTTTGAGGTC CACGAGAAGA CCTACCTCAA GAACATTTTG 
AACAGCATCC GATTCAGCAT CCAGTTGTCT GTCAAGAAGA TCCGACAGGA GGTGGACAAA 
TCCACGTGGC TGCTCCCTCC GCAGGCGCTC AATGCCTACT ATCTGCCCAA CAAGAACCAA 
ATGGTATTCC CAGCTGGCAT CTTGCAGCCC ACCCTGTATG ACCCAGACTT CCCGCAGTCT 
TTGAACTACG GGGGCATCGG TACCATCATT GGGCATGAAC TGACCCACGG CTATGATGAC 
TGGGGAGGAC AGTATGACCG CTCAGGAAAC CTGCTGCACT GGTGGACTGA GGCCTCCTAC 
AGCCGCTTTC TGCACAAGGC CGAGTGCATT GTTCGCCTCT ATGACAACTT CACCGTCTAC 
AACCAGCGGG TGAATGGGAA ACACACACTT GGCGAGAACA TCGCAGACAT GGGAGGCCTC 
AAGCTGGCCT ACTATGCCTA TCAGAAGTGG GTCCGGGAGC ATGGCCCAGA ACACCCGCTG 
CACCGGCTCA AGTACACCCA CAACCAGCTT TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGAC GGTCACAGTC CATCTACCTG CAGGTGCTGA CAGACAAACA CGCACCTGAG 
CACTACCGGG TCCTGGGTAG CGTGTCCCAG TTTGAGGAAT TCGGCCGGGC CTTCCACTGT 
CCCAAGGACT CTCCCATGAA CCCTGTCCAT AAATGCTCTG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuronal injury-inducible gene is synergistically regulated by ATF3, c-Jun, and STAT3 through the interaction with Sp1 in damaged neurons.
The Journal of biological chemistry283(11)6988-96(2008 Mar)
Kiryu-Seo S,Kato R,Ogawa T,Nakagomi S,Nagata K,Kiyama H

Localization and ontogeny of damage-induced neuronal endopeptidase mRNA-expressing neurons in the rat nervous system.
Neuroscience141(1)299-310(2006 Aug)
Nagata K,Kiryu-Seo S,Kiyama H

Damage-induced neuronal endopeptidase (DINE/ECEL) expression is regulated by leukemia inhibitory factor and deprivation of nerve growth factor in rat sensory ganglia after nerve injury.
The Journal of neuroscience : the official journal of the Society for Neuroscience22(21)9410-8(2002 Nov)
Kato R,Kiryu-Seo S,Kiyama H

Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengers.
Proceedings of the National Academy of Sciences of the United States of America97(8)4345-50(2000 Apr)
Kiryu-Seo S,Sasaki M,Yokohama H,Nakagomi S,Hirayama T,Aoki S,Wada K,Kiyama H

Neonatal lethality in mice deficient in XCE, a novel member of the endothelin-converting enzyme and neutral endopeptidase family.
The Journal of biological chemistry274(29)20450-6(1999 Jul)
Schweizer A,Valdenaire O,K?ster A,Lang Y,Schmitt G,Lenz B,Bluethmann H,Rohrer J

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Ecel1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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