GNRHR cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNRHR
Entrez Gene ID	403718
Full Name	gonadotropin releasing hormone receptor
General protein information	Preferred Names gonadotropin releasing hormone receptor Names gonadotropin-releasing hormone receptor GnRH receptor gnRH-R
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003121.1	NP_001003121.1	gonadotropin-releasing hormone receptor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GNRHR	NP_803480.1
Mus musculus (house mouse)	Gnrhr	NP_034453.1
Rattus norvegicus (Norway rat)	Gnrhr	NP_112300.2
Pan troglodytes (chimpanzee)	GNRHR	XP_526608.1
Homo sapiens (human)	GNRHR	NP_000397.1
	GNRHR	XP_001109227.1
Canis lupus familiaris (dog)	GNRHR	NP_001003121.1

Gene Ontology
Bibliography

Related articles in PubMed

GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus.
Sch?fer-Somi S, Kowalewski MP, Kanca H, Bozkurt MF, Gram A, Sabitzer S, Kucukaslan I, Ay SS, Aslan S
Theriogenology84(9)1482-9(2015 Dec)

Molecular cloning, sequencing, and distribution of feline GnRH receptor (GnRHR) and resequencing of canine GnRHR.
Samoylov AM, Napier ID, Morrison NE, Martin DR, Cox NR, Samoylova TI
Theriogenology83(2)266-75(2015 Jan)

Comparative quantitative assessment of GnRH- and LH-receptor mRNA expression in the urinary tract of sexually intact and spayed female dogs.
Reichler IM, Welle M, Sattler U, J?chle W, Roos M, Hubler M, Barth A, Arnold S
Theriogenology67(6)1134-42(2007 Apr)

Immunohistochemical localization and quantitative assessment of GnRH-, FSH-, and LH-receptor mRNA Expression in canine skin: a powerful tool to study the pathogenesis of side effects after spaying.
Welle MM, Reichler IM, Barth A, Forster U, Sattler U, Arnold S
Histochemistry and cell biology126(5)527-35(2006 Nov)

Identification of Phe313 of the gonadotropin-releasing hormone (GnRH) receptor as a site critical for the binding of nonpeptide GnRH antagonists.
Cui J, Smith RG, Mount GR, Lo JL, Yu J, Walsh TF, Singh SB, DeVita RJ, Goulet MT, Schaeffer JM, Cheng K
Molecular endocrinology (Baltimore, Md.)14(5)671-81(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. These findings suggest a local regulatory function of gonadotropin releasing hormone during early canine pregnancy.
Title: GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus.

resequencing of canine gonadotropin-releasing hormone receptor differed from that previously published by a single base change that translates to a different amino acid in position 193
Title: Molecular cloning, sequencing, and distribution of feline GnRH receptor (GnRHR) and resequencing of canine GnRHR.

Age was negatively correlated to mRNA expression of the LH and the GnRH receptors in urinary tract.
Title: Comparative quantitative assessment of GnRH- and LH-receptor mRNA expression in the urinary tract of sexually intact and spayed female dogs.

Immunohistochemical studies showed that GnRH-receptors are expressed in vessel walls, the epidermis, the hair follicle and in sebaceous and sweat glands in canine skin and in transitional epithelium, and smooth muscle tissue in the urinary bladder.
Title: Immunohistochemical localization and quantitative assessment of GnRH-, FSH-, and LH-receptor mRNA Expression in canine skin: a powerful tool to study the pathogenesis of side effects after spaying.

The following GNRHR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNRHR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00197	NM_001003121.1 Latest version!	Canis lupus familiaris gonadotropin releasing hormone receptor (GNRHR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNRHR ( NM_001003121.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003121.1 Canis lupus familiaris gonadotropin releasing hormone receptor (GNRHR), mRNA.

Price & Availability↑

CloneID	OCe00197
Clone ID Related Accession (Same CDS sequence)	NM_001003121.1
Accession Version	NM_001003121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	gonadotropin-releasing hormone receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF206513.1. ##Evidence-Data-START## Transcript exon combination :: AF206513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN07419812 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAGCG CCTCTCCTGA ACAGAATCAA AATCACTGCT CAGCTGTAAA CAACAGCAAC 
ATGCTGATGC AGGGCAACCT CCCCACCCTG ACCTTATCTG GGAAGATCCG AGTGACAGTT 
ACTTTCTTCC TTTTTCTTCT CTCTACAATT TTTAATGCTT CTTTCTTGTT GAAACTTCAG 
AAGTGGACTC AAAAGAAAGA GAAAGGGAAG AAGCTCTCGA GAATGAAAGT GCTTTTAAAA 
CATCTGACCT TGGCCAACCT GTTGGAGACC CTGATTGTCA TGCCACTGGA TGGAATGTGG 
AACATTACAG TCCAATGGTA TGCTGGAGAG TTCCTCTGCA AAGTCCTCAG CTATCTGAAG 
CTTTTCTCCA TGTATGCCCC AGCCTTCATG ATGGTAGTGA TCAGCCTGGA CCGCTCCCTG 
GCCATCACGA GGCCTCTAGC TATGAAAAAC AATGGCAAGC TCGGACAGTC CATGATTGGC 
CTGGCCTGGC TCCTCAGTGG TATCTTTGCT GGACCACAGT TGTACATCTT CAGGATGATC 
CACTTAGCAG ATAGCTCTGG ACAGACAGAG GGTTTCCCCC AATGTGTAAC ACACTGCAGT 
TTTCCACAAT GGTGGCATCA AGCCTTTTAT AACTTCTTCA CCTTCAGCTG CCTCTTCATC 
ATCCCTCTTT TCATCACGTT AATCTGCAAT GCAAAAATCA TATTTACTCT GACACGAGTC 
CTTCATCAGG ATCCCCATGA ATTACAACTG AATCAGTCCA AGAATAATAT ACCAAGAGCT 
CGGCTGAGGA CCTTGAAAAT GACAGTTGCA TTTGCCACTT CGTTTACTGT GTGCTGGACT 
CCCTACTATG TTCTAGGAAT TTGGTACTGG TTTGATCCTG AAATGTTAAA CAGGGTGTCA 
GATCCAGTAA ATCACTTCTT CTTTCTTTTT GCTCTTTTAA ATCCATGCTT TGATCCACTT 
ATATATGGAT ATTTCTCTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003121.1
CDS	1..984
Translation	MASASPEQNQNHCSAVNNSNMLMQGNLPTLTLSGKIRVTVTFFLFLLSTIFNASFLLKLQKWTQKKEKGKKLSRMKVLLKHLTLANLLETLIVMPLDGMWNITVQWYAGEFLCKVLSYLKLFSMYAPAFMMVVISLDRSLAITRPLAMKNNGKLGQSMIGLAWLLSGIFAGPQLYIFRMIHLADSSGQTEGFPQCVTHCSFPQWWHQAFYNFFTFSCLFIIPLFITLICNAKIIFTLTRVLHQDPHELQLNQSKNNIPRARLRTLKMTVAFATSFTVCWTPYYVLGIWYWFDPEMLNRVSDPVNHFFFLFALLNPCFDPLIYGYFSL

Target ORF information:

RefSeq Version	NM_001003121.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gonadotropin releasing hormone receptor (GNRHR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003121.1

ORF Insert Sequence:

ATGGCAAGCG CCTCTCCTGA ACAGAATCAA AATCACTGCT CAGCTGTAAA CAACAGCAAC 
ATGCTGATGC AGGGCAACCT CCCCACCCTG ACCTTATCTG GGAAGATCCG AGTGACAGTT 
ACTTTCTTCC TTTTTCTTCT CTCTACAATT TTTAATGCTT CTTTCTTGTT GAAACTTCAG 
AAGTGGACTC AAAAGAAAGA GAAAGGGAAG AAGCTCTCGA GAATGAAAGT GCTTTTAAAA 
CATCTGACCT TGGCCAACCT GTTGGAGACC CTGATTGTCA TGCCACTGGA TGGAATGTGG 
AACATTACAG TCCAATGGTA TGCTGGAGAG TTCCTCTGCA AAGTCCTCAG CTATCTGAAG 
CTTTTCTCCA TGTATGCCCC AGCCTTCATG ATGGTAGTGA TCAGCCTGGA CCGCTCCCTG 
GCCATCACGA GGCCTCTAGC TATGAAAAAC AATGGCAAGC TCGGACAGTC CATGATTGGC 
CTGGCCTGGC TCCTCAGTGG TATCTTTGCT GGACCACAGT TGTACATCTT CAGGATGATC 
CACTTAGCAG ATAGCTCTGG ACAGACAGAG GGTTTCCCCC AATGTGTAAC ACACTGCAGT 
TTTCCACAAT GGTGGCATCA AGCCTTTTAT AACTTCTTCA CCTTCAGCTG CCTCTTCATC 
ATCCCTCTTT TCATCACGTT AATCTGCAAT GCAAAAATCA TATTTACTCT GACACGAGTC 
CTTCATCAGG ATCCCCATGA ATTACAACTG AATCAGTCCA AGAATAATAT ACCAAGAGCT 
CGGCTGAGGA CCTTGAAAAT GACAGTTGCA TTTGCCACTT CGTTTACTGT GTGCTGGACT 
CCCTACTATG TTCTAGGAAT TTGGTACTGG TTTGATCCTG AAATGTTAAA CAGGGTGTCA 
GATCCAGTAA ATCACTTCTT CTTTCTTTTT GCTCTTTTAA ATCCATGCTT TGATCCACTT 
ATATATGGAT ATTTCTCTCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus.
Theriogenology84(9)1482-9(2015 Dec)
Sch?fer-Somi S,Kowalewski MP,Kanca H,Bozkurt MF,Gram A,Sabitzer S,Kucukaslan I,Ay SS,Aslan S

Molecular cloning, sequencing, and distribution of feline GnRH receptor (GnRHR) and resequencing of canine GnRHR.
Theriogenology83(2)266-75(2015 Jan)
Samoylov AM,Napier ID,Morrison NE,Martin DR,Cox NR,Samoylova TI

Comparative quantitative assessment of GnRH- and LH-receptor mRNA expression in the urinary tract of sexually intact and spayed female dogs.
Theriogenology67(6)1134-42(2007 Apr)
Reichler IM,Welle M,Sattler U,J?chle W,Roos M,Hubler M,Barth A,Arnold S

Immunohistochemical localization and quantitative assessment of GnRH-, FSH-, and LH-receptor mRNA Expression in canine skin: a powerful tool to study the pathogenesis of side effects after spaying.
Histochemistry and cell biology126(5)527-35(2006 Nov)
Welle MM,Reichler IM,Barth A,Forster U,Sattler U,Arnold S

Identification of Phe313 of the gonadotropin-releasing hormone (GnRH) receptor as a site critical for the binding of nonpeptide GnRH antagonists.
Molecular endocrinology (Baltimore, Md.)14(5)671-81(2000 May)
Cui J,Smith RG,Mount GR,Lo JL,Yu J,Walsh TF,Singh SB,DeVita RJ,Goulet MT,Schaeffer JM,Cheng K