NPC1 cDNA ORF clone, Calypte anna(Anna's hummingbird)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPC1
Entrez Gene ID	103531895
Full Name	NPC intracellular cholesterol transporter 1
Gene Type	protein-coding
Organism	Calypte anna(Anna's hummingbird)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008497589.2	XP_008495811.2	NPC intracellular cholesterol transporter 1
XM_008497589.1	XP_008495811.1	Niemann-Pick C1 protein

Pathways from BioSystems
Gene Ontology

The following NPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa423765	XM_008497589.1 Latest version!	Calypte anna Niemann-Pick disease, type C1 (NPC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa812998	XM_008497589.2 Latest version!	Calypte anna NPC intracellular cholesterol transporter 1 (NPC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPC1 ( XM_008497589.1 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_008495811.1 Calypte anna Niemann-Pick disease, type C1 (NPC1), mRNA.

Price & Availability↑

CloneID	OCa423765
Clone ID Related Accession (Same CDS sequence)	XM_008497589.1
Accession Version	XM_008497589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-09
Organism	Calypte anna(Anna's hummingbird)
Product	Niemann-Pick C1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007620712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calypte anna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC CACAAAGCAG CTCGGTGCGG GGCGGCCTCG GCCTGCTCGT CCTCCTCTTC 
CTTCTGCTTC TTTCGCCTTT GCAGGTACTT TCACAATCCT GTGTCTGGTA TGGAGAATGT 
GGAGTTGCTC CTGGAGACAA GAGATACAAC TGTGCTTATG ATGGACCACC AATAGCACTA 
CCAGAGGATG GCTATGAGTT AATGCAGGAA CTCTGTCCAG GCTTATTCTT CGGCAATGTT 
AGCACTTGCT GTGATGTTCG TCAGCTTCAG ACTCTGAAGG ACAACTTGGA GCTGCCTCTG 
CAGTTTCTTT CCAGATGTCC ATCGTGTTTT TACAACTTGA TAAACCTGTT CTGTGAACTG 
ACTTGCAGTC CACATCAATC TGATTTTTTG AACATTACAA GCACCATACC TTATTATGAT 
CCAATTCTAA AAGAGAACAA AAGTAGCATT ACAGAGCTGC AGTACTTTAT AGGAGAACGT 
TTTTCAAATG CAATGTATAA TGCCTGCAAA GATGTGGAGG CACCATCAAG TAATGTTAAA 
GCACTGGGGC TGCTGTGTGG GAAGGAAGTC AATGACTGCA ATGCAACCAA CTGGATTGAG 
TACATGTTTA GTAAAGACAA TGGGCAAACT CCTTTCAGCA TTATTCCTAT CTTTTCAGAT 
GTTCCTGTCC ATGGAATGAA TCCTATGAAC AATGCTACCA AAGGTTGTAA TGAGTCTATG 
GATGATTCAA CAGGACCATG CAGCTGTCAG GACTGTTCAA TTGTCTGTGG TCCAAAACCT 
CAACCCCCTC CATTGCCTCC TCCTTGGCTT TTGTTTGGCT TGGATGCTAT GTATGTCATC 
ATGTGGATCT CCTACATGGC GTTTCTTCTC ATATTTTTTG GACTCGTGTT TGGAGTCTGG 
TGTTACAGGA GGCATCACTT TGTCTCAGAG TACACACCAA TCGACAGCAA TGTAGCCTTT 
TCTGTGAATT CCCATCGTGA CAATGGAAAA CCTACTTGTG GCGAAAGGCT TGGTGAAAGG 
TTTGAGAATG GCCTAAGGAT GACATTCACA TCATGGGGGG CTTTCTGTGT CAGAAACCCA 
CGTCCTGTTA TCCTCTTCTC TGTGGTCTTC ATTGCAATGT GCTGCTCAGG CTTTGTATAT 
GTAAAAGCAA CTACAAACCC TGTAGATCTC TGGTCATCTC CAAGCAGCCA AGCTCACAAG 
GAGAAGAAGT ATTTTGATAC ACACTTTGGG CCTTTCTTTC GTACTGAACA ACTTATTATT 
CAAGCACCAA AGAGTCATCC AGACATCTAT TCACCTTACC CATCTGGAGC AAATGTACCT 
TTTGGGCCTC CACTTAACAA AGACATTCTC CATCAGGTAC TGGATTTGCA GGATGCTATT 
GTCAGCTTAA CTGCTTCTTT TGACAATGAG ACTGTAATGC TGAAAGATAT TTGCCTGGCT 
CCTCTTGCTC CCTACAACAA CAACTGCACT ATACTGAGTG TGCTCAACTA CTTTCAGAAC 
AGTCATTCTG TTCTAGATCA CAGTATTGGG GACGAGTTCT ATACCTTTGC TGACTACCAC 
AGTCACTTCT TGTACTGTGT TCGGGCTCCA GCATCACTGA ATGACACAAG CTTGCTTCAC 
GACCCCTGTT TAGGAACATT TGGTGGACCT GTATTTCCAT GGCTGGTGTT GGGAGGATAT 
GATGATGAAA ACTATAATAA TGCTACAGCT CTTGTTATTA CTTTTCCTGT CAACAACTAC 
TACAATGATT CCAACAAGCT AATGAAAGCC TTGGCATGGG AAAAAGAGTT TATTAACTTT 
CTGAAGAACT ATGATAATCC AAACTTAACT ATATCATTTT CTGCTGAACG GAGTATTGAA 
GATGAAATCA ATCGGGAAAG CAATAGTGAT ATCCGCACTG TGTTGATAAG CTATATTGTA 
ATGTTTCTGT ACATCTCCAT AGCTTTGGGA CATATCCAGA GCTTTAGAAG ACTTCTGGTG 
GACTCAAAGA TCTCTCTGGG CATCGCAGGC ATCTTGATTG TACTGAGCTC GGTGGCATGT 
TCTATAGGCA TTTTCAGCTA CTTTGGAATC CCACTAACAC TGATTGTGAT AGAAGTAATT 
CCCTTCTTGG TGCTGGCTAT TGGGGTGGAC AATATTTTCA TTATAGTCCA GACACTTCAG 
AGGGATGAGC GCCTTCAGGG TGAAACTCTG GATAAACAGA TTGGCAGAGT TTTGGGAGAT 
GTGGCACCCA GTATGTTTCT CTCATCTTTT TCGGAGACTG TGGCATTCTT TCTTGGAACA 
CTGTCTACGA TGCCAGCGGT TCGCACATTC TCCCTTTTTG CTGGAATGGC TGTGCTCATA 
GACTTTCTTC TTCAAGTTAC TTGCTTTGTA AGTCTCCTGG GCTTGGATAT TAAGCGTCAA 
GAGAGGAATA GACTGGATAT TCTGTGTTGC ATCAAAAGCA ATGAAGACAT GAGTGGAGTC 
CAGCGTTCTG AGAACATCTT ATTTGTGTTC TTCAAAAACC TGTATTCTCC TTATCTGCTC 
AAGGATTGGA TGAGACCAAT AGTAATAGCA GTGTTTGTGG GTGTTCTGTC ATTCAGCGCA 
GCAGTCATAC ACAATGTGGA GGTTGGACTG GATCAGTCTC TGTCAATGCC AGATGACTCC 
TATGTAATGG ATTACTTCAG CCATCTCAGC AAGTACCTGC ATGCAGGTCC TCCTGTCTAC 
TTCGTTCTGG AAGAAGGGCA CAACTACACC TCTTTGGAAG GGCAGAACAT GGTGTGTGGT 
GGAATGGGAT GCAATAATGA TTCACTTGTC CAGCAGCTCT TCGATGCTGC AGAAATTGGT 
AGCTACACAA GGATAGGCTA TGCCCCATCG TCCTGGATTG ATGACTACTT TGACTGGGTG 
AAGCCACAGT CATCCTGTTG CAGAGTCTAC AATAACACTG GACAGTTTTG CAATGCTTCA 
GTCACAGATC CGTCCTGCAT TCGCTGTCGA CCACTGACTC AAGAAGGAAA GCAGAGACCA 
CAGGGCAAAG ATTTCATGAC ATATTTGCCG ATGTTCCTAT CAGACAATCC TAATCCTAAA 
TGTGGCAAAG GAGGACATGC TGCATATAAC TCGGCTGTCA ACTTAATAAA CAACAACTCT 
GATGTCGGAG CTACTTATTT CATGACTTAC CATACTGTAT TGAAAACATC ATCTGATTTT 
ATTGATGCTA TGAAGAAAGC TCGGGCTATA GCAGATAACA TTACTGAATC CATGGGCATC 
AAAGAGAAGA ACTTCCGGGT GTTTCCTTAC AGCGTGTTTT ATGTCTTCTA TGAACAATAC 
TTGACAATTG TGCATGATGC CATCTTTAAC CTCTGCATCT CCTTGGGATC AATATTTCTG 
GTGACAACTG TGCTGCTTGG TTTTGAAGTG TGGGCTGCTG TTGTAGTTTC GATAACTATT 
GCTATGATAA TCATCAATAT GTTTGGAGTG ATGTGGCTCT GGGGCATCAG CTTGAACGCA 
GTTTCATTAG TAAATCTTGT CATGAGCTGT GGTATTGCTG TGGAATTCTG TAGTCATGTG 
ACAAGAGCAT TCACTGTTAG TACTAAGGGC AGTAGAGTGG AGCGTGCTGA AGAAGCCCTT 
TCTCATATGG GCAGTTCAGT TTTCAGTGGT ATCACGCTGA CCAAATTTGG AGGGATTATA 
GTACTGGCTT TTTCAAAATC TCAAATCTTT AAGGTGTTCT ACTTCAGGAT GTATTTAGCT 
ATGGTTCTGC TGGGAGCAAC ACACGGACTA ATATTTCTTC CAGTTCTGCT TAGTTACATA 
GGGCCATCAG TAAATAAAGC TAAAACTCGT GCTGCACAAG AGAGGACCAG AGGTACAGAA 
CGGGAGAGAC TCCTCTACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008495811.1
CDS	1..3864
Translation	MGSPQSSSVRGGLGLLVLLFLLLLSPLQVLSQSCVWYGECGVAPGDKRYNCAYDGPPIALPEDGYELMQELCPGLFFGNVSTCCDVRQLQTLKDNLELPLQFLSRCPSCFYNLINLFCELTCSPHQSDFLNITSTIPYYDPILKENKSSITELQYFIGERFSNAMYNACKDVEAPSSNVKALGLLCGKEVNDCNATNWIEYMFSKDNGQTPFSIIPIFSDVPVHGMNPMNNATKGCNESMDDSTGPCSCQDCSIVCGPKPQPPPLPPPWLLFGLDAMYVIMWISYMAFLLIFFGLVFGVWCYRRHHFVSEYTPIDSNVAFSVNSHRDNGKPTCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYVKATTNPVDLWSSPSSQAHKEKKYFDTHFGPFFRTEQLIIQAPKSHPDIYSPYPSGANVPFGPPLNKDILHQVLDLQDAIVSLTASFDNETVMLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSIGDEFYTFADYHSHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDENYNNATALVITFPVNNYYNDSNKLMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESNSDIRTVLISYIVMFLYISIALGHIQSFRRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGIPLTLIVIEVIPFLVLAIGVDNIFIIVQTLQRDERLQGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFLLQVTCFVSLLGLDIKRQERNRLDILCCIKSNEDMSGVQRSENILFVFFKNLYSPYLLKDWMRPIVIAVFVGVLSFSAAVIHNVEVGLDQSLSMPDDSYVMDYFSHLSKYLHAGPPVYFVLEEGHNYTSLEGQNMVCGGMGCNNDSLVQQLFDAAEIGSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNNTGQFCNASVTDPSCIRCRPLTQEGKQRPQGKDFMTYLPMFLSDNPNPKCGKGGHAAYNSAVNLINNNSDVGATYFMTYHTVLKTSSDFIDAMKKARAIADNITESMGIKEKNFRVFPYSVFYVFYEQYLTIVHDAIFNLCISLGSIFLVTTVLLGFEVWAAVVVSITIAMIIINMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIIVLAFSKSQIFKVFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERTRGTERERLLYF

Target ORF information:

RefSeq Version	XM_008497589.1
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna Niemann-Pick disease, type C1 (NPC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008497589.1

ORF Insert Sequence:

ATGGGGAGCC CACAAAGCAG CTCGGTGCGG GGCGGCCTCG GCCTGCTCGT CCTCCTCTTC 
CTTCTGCTTC TTTCGCCTTT GCAGGTACTT TCACAATCCT GTGTCTGGTA TGGAGAATGT 
GGAGTTGCTC CTGGAGACAA GAGATACAAC TGTGCTTATG ATGGACCACC AATAGCACTA 
CCAGAGGATG GCTATGAGTT AATGCAGGAA CTCTGTCCAG GCTTATTCTT CGGCAATGTT 
AGCACTTGCT GTGATGTTCG TCAGCTTCAG ACTCTGAAGG ACAACTTGGA GCTGCCTCTG 
CAGTTTCTTT CCAGATGTCC ATCGTGTTTT TACAACTTGA TAAACCTGTT CTGTGAACTG 
ACTTGCAGTC CACATCAATC TGATTTTTTG AACATTACAA GCACCATACC TTATTATGAT 
CCAATTCTAA AAGAGAACAA AAGTAGCATT ACAGAGCTGC AGTACTTTAT AGGAGAACGT 
TTTTCAAATG CAATGTATAA TGCCTGCAAA GATGTGGAGG CACCATCAAG TAATGTTAAA 
GCACTGGGGC TGCTGTGTGG GAAGGAAGTC AATGACTGCA ATGCAACCAA CTGGATTGAG 
TACATGTTTA GTAAAGACAA TGGGCAAACT CCTTTCAGCA TTATTCCTAT CTTTTCAGAT 
GTTCCTGTCC ATGGAATGAA TCCTATGAAC AATGCTACCA AAGGTTGTAA TGAGTCTATG 
GATGATTCAA CAGGACCATG CAGCTGTCAG GACTGTTCAA TTGTCTGTGG TCCAAAACCT 
CAACCCCCTC CATTGCCTCC TCCTTGGCTT TTGTTTGGCT TGGATGCTAT GTATGTCATC 
ATGTGGATCT CCTACATGGC GTTTCTTCTC ATATTTTTTG GACTCGTGTT TGGAGTCTGG 
TGTTACAGGA GGCATCACTT TGTCTCAGAG TACACACCAA TCGACAGCAA TGTAGCCTTT 
TCTGTGAATT CCCATCGTGA CAATGGAAAA CCTACTTGTG GCGAAAGGCT TGGTGAAAGG 
TTTGAGAATG GCCTAAGGAT GACATTCACA TCATGGGGGG CTTTCTGTGT CAGAAACCCA 
CGTCCTGTTA TCCTCTTCTC TGTGGTCTTC ATTGCAATGT GCTGCTCAGG CTTTGTATAT 
GTAAAAGCAA CTACAAACCC TGTAGATCTC TGGTCATCTC CAAGCAGCCA AGCTCACAAG 
GAGAAGAAGT ATTTTGATAC ACACTTTGGG CCTTTCTTTC GTACTGAACA ACTTATTATT 
CAAGCACCAA AGAGTCATCC AGACATCTAT TCACCTTACC CATCTGGAGC AAATGTACCT 
TTTGGGCCTC CACTTAACAA AGACATTCTC CATCAGGTAC TGGATTTGCA GGATGCTATT 
GTCAGCTTAA CTGCTTCTTT TGACAATGAG ACTGTAATGC TGAAAGATAT TTGCCTGGCT 
CCTCTTGCTC CCTACAACAA CAACTGCACT ATACTGAGTG TGCTCAACTA CTTTCAGAAC 
AGTCATTCTG TTCTAGATCA CAGTATTGGG GACGAGTTCT ATACCTTTGC TGACTACCAC 
AGTCACTTCT TGTACTGTGT TCGGGCTCCA GCATCACTGA ATGACACAAG CTTGCTTCAC 
GACCCCTGTT TAGGAACATT TGGTGGACCT GTATTTCCAT GGCTGGTGTT GGGAGGATAT 
GATGATGAAA ACTATAATAA TGCTACAGCT CTTGTTATTA CTTTTCCTGT CAACAACTAC 
TACAATGATT CCAACAAGCT AATGAAAGCC TTGGCATGGG AAAAAGAGTT TATTAACTTT 
CTGAAGAACT ATGATAATCC AAACTTAACT ATATCATTTT CTGCTGAACG GAGTATTGAA 
GATGAAATCA ATCGGGAAAG CAATAGTGAT ATCCGCACTG TGTTGATAAG CTATATTGTA 
ATGTTTCTGT ACATCTCCAT AGCTTTGGGA CATATCCAGA GCTTTAGAAG ACTTCTGGTG 
GACTCAAAGA TCTCTCTGGG CATCGCAGGC ATCTTGATTG TACTGAGCTC GGTGGCATGT 
TCTATAGGCA TTTTCAGCTA CTTTGGAATC CCACTAACAC TGATTGTGAT AGAAGTAATT 
CCCTTCTTGG TGCTGGCTAT TGGGGTGGAC AATATTTTCA TTATAGTCCA GACACTTCAG 
AGGGATGAGC GCCTTCAGGG TGAAACTCTG GATAAACAGA TTGGCAGAGT TTTGGGAGAT 
GTGGCACCCA GTATGTTTCT CTCATCTTTT TCGGAGACTG TGGCATTCTT TCTTGGAACA 
CTGTCTACGA TGCCAGCGGT TCGCACATTC TCCCTTTTTG CTGGAATGGC TGTGCTCATA 
GACTTTCTTC TTCAAGTTAC TTGCTTTGTA AGTCTCCTGG GCTTGGATAT TAAGCGTCAA 
GAGAGGAATA GACTGGATAT TCTGTGTTGC ATCAAAAGCA ATGAAGACAT GAGTGGAGTC 
CAGCGTTCTG AGAACATCTT ATTTGTGTTC TTCAAAAACC TGTATTCTCC TTATCTGCTC 
AAGGATTGGA TGAGACCAAT AGTAATAGCA GTGTTTGTGG GTGTTCTGTC ATTCAGCGCA 
GCAGTCATAC ACAATGTGGA GGTTGGACTG GATCAGTCTC TGTCAATGCC AGATGACTCC 
TATGTAATGG ATTACTTCAG CCATCTCAGC AAGTACCTGC ATGCAGGTCC TCCTGTCTAC 
TTCGTTCTGG AAGAAGGGCA CAACTACACC TCTTTGGAAG GGCAGAACAT GGTGTGTGGT 
GGAATGGGAT GCAATAATGA TTCACTTGTC CAGCAGCTCT TCGATGCTGC AGAAATTGGT 
AGCTACACAA GGATAGGCTA TGCCCCATCG TCCTGGATTG ATGACTACTT TGACTGGGTG 
AAGCCACAGT CATCCTGTTG CAGAGTCTAC AATAACACTG GACAGTTTTG CAATGCTTCA 
GTCACAGATC CGTCCTGCAT TCGCTGTCGA CCACTGACTC AAGAAGGAAA GCAGAGACCA 
CAGGGCAAAG ATTTCATGAC ATATTTGCCG ATGTTCCTAT CAGACAATCC TAATCCTAAA 
TGTGGCAAAG GAGGACATGC TGCATATAAC TCGGCTGTCA ACTTAATAAA CAACAACTCT 
GATGTCGGAG CTACTTATTT CATGACTTAC CATACTGTAT TGAAAACATC ATCTGATTTT 
ATTGATGCTA TGAAGAAAGC TCGGGCTATA GCAGATAACA TTACTGAATC CATGGGCATC 
AAAGAGAAGA ACTTCCGGGT GTTTCCTTAC AGCGTGTTTT ATGTCTTCTA TGAACAATAC 
TTGACAATTG TGCATGATGC CATCTTTAAC CTCTGCATCT CCTTGGGATC AATATTTCTG 
GTGACAACTG TGCTGCTTGG TTTTGAAGTG TGGGCTGCTG TTGTAGTTTC GATAACTATT 
GCTATGATAA TCATCAATAT GTTTGGAGTG ATGTGGCTCT GGGGCATCAG CTTGAACGCA 
GTTTCATTAG TAAATCTTGT CATGAGCTGT GGTATTGCTG TGGAATTCTG TAGTCATGTG 
ACAAGAGCAT TCACTGTTAG TACTAAGGGC AGTAGAGTGG AGCGTGCTGA AGAAGCCCTT 
TCTCATATGG GCAGTTCAGT TTTCAGTGGT ATCACGCTGA CCAAATTTGG AGGGATTATA 
GTACTGGCTT TTTCAAAATC TCAAATCTTT AAGGTGTTCT ACTTCAGGAT GTATTTAGCT 
ATGGTTCTGC TGGGAGCAAC ACACGGACTA ATATTTCTTC CAGTTCTGCT TAGTTACATA 
GGGCCATCAG TAAATAAAGC TAAAACTCGT GCTGCACAAG AGAGGACCAG AGGTACAGAA 
CGGGAGAGAC TCCTCTACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPC1 ( XM_008497589.2 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_008495811.2 Calypte anna NPC intracellular cholesterol transporter 1 (NPC1), mRNA.

Price & Availability↑

CloneID	OCa812998
Clone ID Related Accession (Same CDS sequence)	XM_008497589.2
Accession Version	XM_008497589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-19
Organism	Calypte anna(Anna's hummingbird)
Product	NPC intracellular cholesterol transporter 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044245.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 20, 2019 this sequence version replaced XM_008497589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Calypte anna Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC CACAAAGCAG CTCGGTGCGG GGCGGCCTCG GCCTGCTCGT CCTCCTCTTC 
CTTCTGCTTC TTTCGCCTTT GCAGGTACTT TCACAATCCT GTGTCTGGTA TGGAGAATGT 
GGAGTTGCTC CTGGAGACAA GAGATACAAC TGTGCTTATG ATGGACCACC AATAGCACTA 
CCAGAGGATG GCTATGAGTT AATGCAGGAA CTCTGTCCAG GCTTATTCTT CGGCAATGTT 
AGCACTTGCT GTGATGTTCG TCAGCTTCAG ACTCTGAAGG ACAACTTGGA GCTGCCTCTG 
CAGTTTCTTT CCAGATGTCC ATCGTGTTTT TACAACTTGA TAAACCTGTT CTGTGAACTG 
ACTTGCAGTC CACATCAATC TGATTTTTTG AACGTTACAA GCACCATACC TTATTATGAT 
CCAATTCTAA AAGAGAACAA AAGTAGCATT ACAGAGCTGC AGTACTTTAT AGGAGAACGT 
TTTTCAAATG CAATGTATAA TGCCTGCAAA GATGTGGAGG CACCATCAAG TAATGTTAAA 
GCACTGGGGC TGCTGTGTGG GAAGGAAGTC AATGACTGCA ATGCAACCAA CTGGATTGAG 
TACATGTTTA GTAAAGACAA TGGGCAAACT CCTTTCAGCA TTATTCCTAT CTTTTCAGAT 
GTTCCTGTCC ATGGAATGAA TCCTATGAAC AATGCTACCA AAGGTTGTAA TGAGTCTATG 
GATGATTCAA CAGGACCATG CAGCTGTCAG GACTGTTCAA TTGTCTGTGG TCCAAAACCT 
CAACCCCCTC CATTGCCTCC TCCTTGGCTT TTGTTTGGCT TGGATGCTAT GTATGTCATC 
ATGTGGATCT CCTACATGGC ATTTCTTCTC ATATTTTTTG GACTCGTGTT TGGAGTCTGG 
TGTTACAGGA GGCATCACTT TGTCTCAGAG TACACACCAA TCGACAGCAA TGTAGCCTTT 
TCTGTGAATT CCCATCGTGA CAATGGAAAA CCTACTTGTG GCGAAAGGCT TGGTGAAAGG 
TTTGAGAATG GCCTAAGGAT GACATTCACA TCATGGGGGG CTTTCTGTGT CAGAAACCCA 
CGTCCTGTTA TCCTCTTCTC TGTGGTCTTC ATTGCAATGT GCTGCTCAGG CTTTGTATAT 
GTAAAAGCAA CTACAAACCC TGTAGATCTC TGGTCATCTC CAAGCAGCCA AGCTCACAAG 
GAGAAGAAGT ATTTTGATAC ACACTTTGGG CCTTTCTTTC GTACTGAACA ACTTATTATT 
CAAGCACCAA AGAGTCATCC AGACATCTAT TCACCTTACC CATCTGGAGC AAATGTACCT 
TTTGGGCCTC CACTTAACAA AGACATTCTC CATCAGGTAC TGGATTTGCA GGATGCTATT 
GTCAGCTTAA CTGCTTCTTT TGACAATGAG ACTGTAATGC TGAAAGATAT TTGCCTGGCT 
CCTCTTGCTC CCTACAACAA CAACTGCACT ATACTGAGTG TGCTCAACTA CTTTCAGAAC 
AGTCATTCTG TTCTAGATCA CAGTATTGGG GACGAGTTCT ATACCTTTGC TGACTACCAC 
AGTCACTTCT TGTACTGTGT TCGGGCTCCA GCATCACTGA ATGACACAAG CTTGCTTCAC 
GACCCCTGTT TAGGAACATT TGGTGGACCT GTATTTCCAT GGCTGGTGTT GGGAGGATAT 
GATGATGAAA ACTATAATAA TGCTACAGCT CTTGTTATTA CTTTTCCTGT CAACAACTAC 
TACAATGATT CCAACAAGCT AATGAAAGCC TTGGCATGGG AAAAAGAGTT TATTAACTTT 
CTGAAGAACT ATGATAATCC AAACTTAACT ATATCATTTT CTGCTGAACG GAGTATTGAA 
GATGAAATCA ATCGGGAAAG CAATAGTGAT ATCCGCACTG TGTTGATAAG CTATATTGTA 
ATGTTTCTGT ACATCTCCAT AGCTTTGGGA CATATCCAGA GCTTTAGAAG ACTTCTGGTG 
GACTCAAAGA TCTCTCTGGG CATCGCAGGC ATCTTGATTG TACTGAGCTC GGTGGCATGT 
TCTATAGGCA TTTTCAGCTA CTTTGGAATC CCACTAACAC TGATTGTGAT AGAAGTAATT 
CCCTTCTTGG TGCTGGCTAT TGGGGTGGAC AATATTTTCA TTATAGTCCA GACACTTCAG 
AGGGATGAGC GCCTTCAGGG TGAAACTCTG GATAAACAGA TTGGCAGAGT TTTGGGAGAT 
GTGGCACCCA GTATGTTTCT CTCATCTTTT TCGGAGACTG TGGCATTCTT TCTTGGAACA 
CTGTCTACGA TGCCAGCGGT TCGCACATTC TCCCTTTTTG CTGGAATGGC TGTGCTCATA 
GACTTTCTTC TTCAAGTTAC TTGCTTTGTA AGTCTCCTGG GCTTGGATAT TAAGCGTCAA 
GAGAGGAATA GACTGGATAT TCTGTGTTGC ATCAAAAGCA ATGAAGACAT GAGTGGAGTC 
CAGCGTTCTG AGAACATCTT ATTTGTGTTC TTCAAAAACC TGTATTCTCC TTATCTGCTC 
AAGGATTGGA TGAGACCAAT AGTAATAGCA GTGTTTGTGG GTGTTCTGTC ATTCAGCGCA 
GCAGTCATAC ACAATGTGGA GGTTGGACTG GATCAGTCTC TGTCAATGCC AGATGACTCC 
TATGTAATGG ATTACTTCAG CCATCTCAGC AAGTACCTGC ATGCAGGTCC TCCTGTCTAC 
TTCGTTCTGG AAGAAGGGCA CAACTACACC TCTTTGGAAG GGCAGAACAT GGTGTGTGGT 
GGAATGGGAT GCAATAATGA TTCACTTGTC CAGCAGCTCT TCGATGCTGC AGAAATTGGT 
AGCTACACAA GGATAGGCTA TGCCCCATCG TCCTGGATTG ATGACTACTT TGACTGGGTG 
AAGCCACAGT CATCCTGTTG CAGAGTCTAC AATAACACTG GACAGTTTTG CAATGCTTCA 
GTCACAGATC CGTCCTGCAT TCGCTGTCGA CCACTGACTC AAGAAGGAAA GCAGAGACCA 
CAGGGCAAAG ATTTCATGAC ATATTTGCCG ATGTTCCTAT CAGACAATCC TAATCCTAAG 
TGTGGCAAAG GAGGACATGC TGCATATAAC TCGGCTGTCA ACTTAATAAA CAACAACTCT 
GATGTCGGAG CTACTTATTT CATGACTTAC CATACTGTAT TGAAAACATC ATCTGATTTT 
ATTGATGCTA TGAAGAAAGC TCGGGCTATA GCAGATAACA TTACTGAATC CATGGGCATC 
AAAGAGAAGA ACTTCCGGGT GTTTCCTTAC AGCGTGTTTT ATGTCTTCTA TGAACAATAC 
TTGACAATTG TGCATGATGC CATCTTTAAC CTCTGCATCT CCTTGGGATC AATATTTCTG 
GTGACAACTG TGCTGCTTGG TTTTGAAGTG TGGGCTGCTG TTGTAGTTTC GATAACTATT 
GCTATGATAA TCATCAATAT GTTTGGAGTG ATGTGGCTCT GGGGCATCAG CTTGAACGCA 
GTTTCATTAG TAAATCTTGT CATGAGCTGT GGTATTGCTG TGGAATTCTG TAGTCATGTG 
ACAAGAGCAT TCACTGTTAG TACTAAGGGC AGTAGAGTGG AGCGTGCTGA AGAAGCCCTT 
TCTCACATGG GCAGTTCAGT TTTCAGTGGT ATCACGCTGA CCAAATTTGG AGGGATTATA 
GTACTGGCTT TTTCAAAATC TCAAATCTTT AAGGTGTTCT ACTTCAGGAT GTATTTAGCT 
ATGGTTCTGC TGGGAGCAAC ACACGGACTA ATATTTCTTC CAGTTCTGCT TAGTTACATA 
GGGCCATCAG TAAATAAAGC TAAAACTCGT GCTGCACAAG AGAGGACCAG AGGTACAGAA 
CGGGAGAGAC TCCTCTACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008495811.2
CDS	145..4008
Translation	MGSPQSSSVRGGLGLLVLLFLLLLSPLQVLSQSCVWYGECGVAPGDKRYNCAYDGPPIALPEDGYELMQELCPGLFFGNVSTCCDVRQLQTLKDNLELPLQFLSRCPSCFYNLINLFCELTCSPHQSDFLNVTSTIPYYDPILKENKSSITELQYFIGERFSNAMYNACKDVEAPSSNVKALGLLCGKEVNDCNATNWIEYMFSKDNGQTPFSIIPIFSDVPVHGMNPMNNATKGCNESMDDSTGPCSCQDCSIVCGPKPQPPPLPPPWLLFGLDAMYVIMWISYMAFLLIFFGLVFGVWCYRRHHFVSEYTPIDSNVAFSVNSHRDNGKPTCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYVKATTNPVDLWSSPSSQAHKEKKYFDTHFGPFFRTEQLIIQAPKSHPDIYSPYPSGANVPFGPPLNKDILHQVLDLQDAIVSLTASFDNETVMLKDICLAPLAPYNNNCTILSVLNYFQNSHSVLDHSIGDEFYTFADYHSHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDENYNNATALVITFPVNNYYNDSNKLMKALAWEKEFINFLKNYDNPNLTISFSAERSIEDEINRESNSDIRTVLISYIVMFLYISIALGHIQSFRRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGIPLTLIVIEVIPFLVLAIGVDNIFIIVQTLQRDERLQGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFLLQVTCFVSLLGLDIKRQERNRLDILCCIKSNEDMSGVQRSENILFVFFKNLYSPYLLKDWMRPIVIAVFVGVLSFSAAVIHNVEVGLDQSLSMPDDSYVMDYFSHLSKYLHAGPPVYFVLEEGHNYTSLEGQNMVCGGMGCNNDSLVQQLFDAAEIGSYTRIGYAPSSWIDDYFDWVKPQSSCCRVYNNTGQFCNASVTDPSCIRCRPLTQEGKQRPQGKDFMTYLPMFLSDNPNPKCGKGGHAAYNSAVNLINNNSDVGATYFMTYHTVLKTSSDFIDAMKKARAIADNITESMGIKEKNFRVFPYSVFYVFYEQYLTIVHDAIFNLCISLGSIFLVTTVLLGFEVWAAVVVSITIAMIIINMFGVMWLWGISLNAVSLVNLVMSCGIAVEFCSHVTRAFTVSTKGSRVERAEEALSHMGSSVFSGITLTKFGGIIVLAFSKSQIFKVFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKTRAAQERTRGTERERLLYF

Target ORF information:

RefSeq Version	XM_008497589.2
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna NPC intracellular cholesterol transporter 1 (NPC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008497589.2

ORF Insert Sequence:

ATGGGGAGCC CACAAAGCAG CTCGGTGCGG GGCGGCCTCG GCCTGCTCGT CCTCCTCTTC 
CTTCTGCTTC TTTCGCCTTT GCAGGTACTT TCACAATCCT GTGTCTGGTA TGGAGAATGT 
GGAGTTGCTC CTGGAGACAA GAGATACAAC TGTGCTTATG ATGGACCACC AATAGCACTA 
CCAGAGGATG GCTATGAGTT AATGCAGGAA CTCTGTCCAG GCTTATTCTT CGGCAATGTT 
AGCACTTGCT GTGATGTTCG TCAGCTTCAG ACTCTGAAGG ACAACTTGGA GCTGCCTCTG 
CAGTTTCTTT CCAGATGTCC ATCGTGTTTT TACAACTTGA TAAACCTGTT CTGTGAACTG 
ACTTGCAGTC CACATCAATC TGATTTTTTG AACGTTACAA GCACCATACC TTATTATGAT 
CCAATTCTAA AAGAGAACAA AAGTAGCATT ACAGAGCTGC AGTACTTTAT AGGAGAACGT 
TTTTCAAATG CAATGTATAA TGCCTGCAAA GATGTGGAGG CACCATCAAG TAATGTTAAA 
GCACTGGGGC TGCTGTGTGG GAAGGAAGTC AATGACTGCA ATGCAACCAA CTGGATTGAG 
TACATGTTTA GTAAAGACAA TGGGCAAACT CCTTTCAGCA TTATTCCTAT CTTTTCAGAT 
GTTCCTGTCC ATGGAATGAA TCCTATGAAC AATGCTACCA AAGGTTGTAA TGAGTCTATG 
GATGATTCAA CAGGACCATG CAGCTGTCAG GACTGTTCAA TTGTCTGTGG TCCAAAACCT 
CAACCCCCTC CATTGCCTCC TCCTTGGCTT TTGTTTGGCT TGGATGCTAT GTATGTCATC 
ATGTGGATCT CCTACATGGC ATTTCTTCTC ATATTTTTTG GACTCGTGTT TGGAGTCTGG 
TGTTACAGGA GGCATCACTT TGTCTCAGAG TACACACCAA TCGACAGCAA TGTAGCCTTT 
TCTGTGAATT CCCATCGTGA CAATGGAAAA CCTACTTGTG GCGAAAGGCT TGGTGAAAGG 
TTTGAGAATG GCCTAAGGAT GACATTCACA TCATGGGGGG CTTTCTGTGT CAGAAACCCA 
CGTCCTGTTA TCCTCTTCTC TGTGGTCTTC ATTGCAATGT GCTGCTCAGG CTTTGTATAT 
GTAAAAGCAA CTACAAACCC TGTAGATCTC TGGTCATCTC CAAGCAGCCA AGCTCACAAG 
GAGAAGAAGT ATTTTGATAC ACACTTTGGG CCTTTCTTTC GTACTGAACA ACTTATTATT 
CAAGCACCAA AGAGTCATCC AGACATCTAT TCACCTTACC CATCTGGAGC AAATGTACCT 
TTTGGGCCTC CACTTAACAA AGACATTCTC CATCAGGTAC TGGATTTGCA GGATGCTATT 
GTCAGCTTAA CTGCTTCTTT TGACAATGAG ACTGTAATGC TGAAAGATAT TTGCCTGGCT 
CCTCTTGCTC CCTACAACAA CAACTGCACT ATACTGAGTG TGCTCAACTA CTTTCAGAAC 
AGTCATTCTG TTCTAGATCA CAGTATTGGG GACGAGTTCT ATACCTTTGC TGACTACCAC 
AGTCACTTCT TGTACTGTGT TCGGGCTCCA GCATCACTGA ATGACACAAG CTTGCTTCAC 
GACCCCTGTT TAGGAACATT TGGTGGACCT GTATTTCCAT GGCTGGTGTT GGGAGGATAT 
GATGATGAAA ACTATAATAA TGCTACAGCT CTTGTTATTA CTTTTCCTGT CAACAACTAC 
TACAATGATT CCAACAAGCT AATGAAAGCC TTGGCATGGG AAAAAGAGTT TATTAACTTT 
CTGAAGAACT ATGATAATCC AAACTTAACT ATATCATTTT CTGCTGAACG GAGTATTGAA 
GATGAAATCA ATCGGGAAAG CAATAGTGAT ATCCGCACTG TGTTGATAAG CTATATTGTA 
ATGTTTCTGT ACATCTCCAT AGCTTTGGGA CATATCCAGA GCTTTAGAAG ACTTCTGGTG 
GACTCAAAGA TCTCTCTGGG CATCGCAGGC ATCTTGATTG TACTGAGCTC GGTGGCATGT 
TCTATAGGCA TTTTCAGCTA CTTTGGAATC CCACTAACAC TGATTGTGAT AGAAGTAATT 
CCCTTCTTGG TGCTGGCTAT TGGGGTGGAC AATATTTTCA TTATAGTCCA GACACTTCAG 
AGGGATGAGC GCCTTCAGGG TGAAACTCTG GATAAACAGA TTGGCAGAGT TTTGGGAGAT 
GTGGCACCCA GTATGTTTCT CTCATCTTTT TCGGAGACTG TGGCATTCTT TCTTGGAACA 
CTGTCTACGA TGCCAGCGGT TCGCACATTC TCCCTTTTTG CTGGAATGGC TGTGCTCATA 
GACTTTCTTC TTCAAGTTAC TTGCTTTGTA AGTCTCCTGG GCTTGGATAT TAAGCGTCAA 
GAGAGGAATA GACTGGATAT TCTGTGTTGC ATCAAAAGCA ATGAAGACAT GAGTGGAGTC 
CAGCGTTCTG AGAACATCTT ATTTGTGTTC TTCAAAAACC TGTATTCTCC TTATCTGCTC 
AAGGATTGGA TGAGACCAAT AGTAATAGCA GTGTTTGTGG GTGTTCTGTC ATTCAGCGCA 
GCAGTCATAC ACAATGTGGA GGTTGGACTG GATCAGTCTC TGTCAATGCC AGATGACTCC 
TATGTAATGG ATTACTTCAG CCATCTCAGC AAGTACCTGC ATGCAGGTCC TCCTGTCTAC 
TTCGTTCTGG AAGAAGGGCA CAACTACACC TCTTTGGAAG GGCAGAACAT GGTGTGTGGT 
GGAATGGGAT GCAATAATGA TTCACTTGTC CAGCAGCTCT TCGATGCTGC AGAAATTGGT 
AGCTACACAA GGATAGGCTA TGCCCCATCG TCCTGGATTG ATGACTACTT TGACTGGGTG 
AAGCCACAGT CATCCTGTTG CAGAGTCTAC AATAACACTG GACAGTTTTG CAATGCTTCA 
GTCACAGATC CGTCCTGCAT TCGCTGTCGA CCACTGACTC AAGAAGGAAA GCAGAGACCA 
CAGGGCAAAG ATTTCATGAC ATATTTGCCG ATGTTCCTAT CAGACAATCC TAATCCTAAG 
TGTGGCAAAG GAGGACATGC TGCATATAAC TCGGCTGTCA ACTTAATAAA CAACAACTCT 
GATGTCGGAG CTACTTATTT CATGACTTAC CATACTGTAT TGAAAACATC ATCTGATTTT 
ATTGATGCTA TGAAGAAAGC TCGGGCTATA GCAGATAACA TTACTGAATC CATGGGCATC 
AAAGAGAAGA ACTTCCGGGT GTTTCCTTAC AGCGTGTTTT ATGTCTTCTA TGAACAATAC 
TTGACAATTG TGCATGATGC CATCTTTAAC CTCTGCATCT CCTTGGGATC AATATTTCTG 
GTGACAACTG TGCTGCTTGG TTTTGAAGTG TGGGCTGCTG TTGTAGTTTC GATAACTATT 
GCTATGATAA TCATCAATAT GTTTGGAGTG ATGTGGCTCT GGGGCATCAG CTTGAACGCA 
GTTTCATTAG TAAATCTTGT CATGAGCTGT GGTATTGCTG TGGAATTCTG TAGTCATGTG 
ACAAGAGCAT TCACTGTTAG TACTAAGGGC AGTAGAGTGG AGCGTGCTGA AGAAGCCCTT 
TCTCACATGG GCAGTTCAGT TTTCAGTGGT ATCACGCTGA CCAAATTTGG AGGGATTATA 
GTACTGGCTT TTTCAAAATC TCAAATCTTT AAGGTGTTCT ACTTCAGGAT GTATTTAGCT 
ATGGTTCTGC TGGGAGCAAC ACACGGACTA ATATTTCTTC CAGTTCTGCT TAGTTACATA 
GGGCCATCAG TAAATAAAGC TAAAACTCGT GCTGCACAAG AGAGGACCAG AGGTACAGAA 
CGGGAGAGAC TCCTCTACTT CTAG