tll1 cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	tll1
Entrez Gene ID	103177344
Full Name	tolloid like 1
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007891468.1	XP_007889659.1	tolloid-like protein 1 isoform X2
XM_007891466.1	XP_007889657.1	tolloid-like protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following tll1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tll1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa374747	XM_007891468.1 Latest version!	Callorhinchus milii tolloid-like 1 (tll1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa374746	XM_007891466.1 Latest version!	Callorhinchus milii tolloid-like 1 (tll1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tll1 ( XM_007891468.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007889659.1 Callorhinchus milii tolloid-like 1 (tll1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa374747
Clone ID Related Accession (Same CDS sequence)	XM_007891468.1
Accession Version	XM_007891468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACGG CAATAGAGCT GCTGCTCCTC TGTTGGGTGA GAAGAGTTGC AGATAGTGAG 
CTGAATAACA CAGATAGAGA AATGAACTCA GAAGCCTTAA AACAGACAGA GAAGAAAAGA 
ATTCCCCGAG CTGCAACTTC GCGAGCGGAG AGAATATGGC CTGGAGGTGT CATTCCATAT 
GTTATTGGAG GGAATTTTAC TGGCAGTCAG CGAGCTATGT TCAAACAGGC CATGCGGCAC 
TGGGAAAAGC ACACGTGTGT TACATTCATC GAACGAACAG AAGAAGAAAG TTACATTGTC 
TTCACGTACC GGCCATGTGG GTGCTGCTCC TATGTGGGTA GACGTGGAAA TGGTCCTCAA 
GCTATATCCA TAGGCAAAAA CTGTGACAAG TTTGGGATTG TGGTCCATGA ATTAGGGCAT 
GTGATTGGGT TTTGGCATGA ACACACAAGA CCAGACCGAG ATGATCATGT AACTATCATT 
CGAGAAAACA TCCAGCCTGG CCAGGAGTAC AACTTCCTCA AGATGGAACC TGGTGAAGTG 
AACTCCCTCG GGGAGTCATA CGACTTTGAC AGTATTATGC ACTACGCTAG GAACACCTTC 
TCAAGAGGAA TGTTTCTGGA CACCATTCTG CCTGAACGTG ACGAAAATGG TGTAAGACCT 
GCCATTGGCC AACGTACACG TTTAAGTAAA GGAGACATTG CACAGGCAAG GAAGCTGTAT 
AGATGTCCAG CGTGTGGGGA AACACTCCAA GACTCGACCA GTAATTTCAC GTCACCAGGA 
TTTCCGAATG GATACCCCTC TTACACACAT TGTGTTTGGA GAATCTCTGT TACACCTGGT 
GAAAAGATTG TGTTAAATTT TACCACCATG GATCTTTTCA AGAGCCGCCT GTGCTGGTAT 
GACTACATAG AAGTACGAGA TGGCTATTGG AGAAAGGCAC CGCTTCTTGG CAGATACTGT 
GGTGATAAAT TGCCTGAAGT CCTGATTTCC ACTGACAGTA GAATGTGGAT AGAATTTCGA 
AGCAGCAGTA ACTGGGTGGG AAAAGGTTTT GCAGCAGTCT ATGAAGCAAT CTGTGGAGGT 
GAGATCCACA AAGAATCCGG GCAGATTCAG TCTCCTAACT ATCCAGATGA CTACAGACCG 
ATGAAGGAGT GTGTATGGAA GATCACCATG TCTGAGCATT ACCATGTAGG ATTAATTTTC 
CAGGCATTTG AGATTGAGAG ACATGACAAT TGTGCCTATG ACTATCTAGA AGTACGAGAT 
GGCAGCAGTG AAAATAGTCC TATGATTGGG CGCTTCTGTG GCTACGACAA ACCAGAGGAC 
ATAAAATCCA CGTCTAATAC CTTGTGGATG AAGTTTGTTT CCGATGGAAC AGTTAATAAA 
GCAGGATTTG CTGCAAATTT CTTTAAAGAG GAGGATGAAT GTGCCAGGCC TGACAACGGA 
GGTTGCGAGC AACGATGTTT GAACACTTTG GGCAGTTATA AATGCACTTG CGATCCAGGC 
TACGAGCTTG CAGCAGACAA GAAGAGCTGT GAAGCTGCAT GCGGAGGACT CCTAACCAAG 
TTAAATGGAA CGATCACCAC ACCAGGCTGG CCTAAAGAAT ACCCTCCAAA TAAAAACTGT 
GTGTGGCAGG TTGTGGCTCC GACCCAATAC AGAATCTCGA TGCAGTTTGA ATTTTTTGAA 
TTAGAAGGCA ATGACATATG CAAGTACGAT TATGTGGAGA TCCGGAGTGG CCTTTCATCC 
GATTCCAAGC TTCATGGCAA GTTTTGCGGT ACGGAAGTGC CTGAAGTTAT AACATCCCGA 
TACAACAACA TGAGGATCGA GTTCAAATCA GACAATACTG TTTCAAAAAA AGGATTCAAA 
GCCCACTTTC TTTCAGACAA GGATGAGTGC TCCAAGGACA ACGGTGGCTG CCAACACGAG 
TGCATCAACA CCATAGGAAG TTATGTTTGC CAGTGTCGTA ATGGATTTGT GCTACATGAA 
AACAAACACG ACTGCAAAGA GGCTGAATGT GAGCACCAGA TCCACAGTGC TACAGGGGTC 
ATTACCAGCC CCAACTGGCC CGATAAATAC CCCAGCCGCA AAGAATGCAC GTGGGAGATC 
TTAGCTACCC CAGGGCACAG GGTGAAAATA ACTTTCAACG AGTTTGACAT TGAACAACAC 
CAGGAATGTG CTTACGATCA TCTGGAAGTG TTTGACGGAG AGTCTGATAA ATATCCAATT 
CTGGGGCGAT TCTGTGGCAG CAAAATGCCA GATCCAGTCC TCTCGACCAC AAACAAAATG 
TTCATTGGAT TCATTTCGGA TGCATCGGTA CAAAGGAAAG GTTTCCAGGC TACCCACTCC 
ACAGAATGTG GTGGCAGACT GAAGGCTGAA ATCAAATCTA AGGATTTGTT TTCTCACGCT 
CAGTTTGGAG ATAACAACTA CCCAGGCCAA GTGGACTGTG AGTGGCAGGT TTTAGCAGAA 
AAAGGCTACA GCGTGGAGCT CTCTTTTCAG ACGTTTGAAG TGGAGGTGGA AGCTGACTGT 
GGCTATGACT TTATTGAACT GTTCGATGGG TTTGATACAA CAGCTTCAAG ACTTGGGCGG 
TTCTGTGGAT CTGGACCACC CGAGGAAATC TATTCAGCGG GAGATGCCAT TTTAATCCAT 
TTCCATACTG ATGACACAAT AAACAAAAAA GGATTCCATA TACGATATAC AAGTACAAAA 
TTCCAGGATG CCATACAGAC CAAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007889659.1
CDS	13..2742
Translation	MGTAIELLLLCWVRRVADSELNNTDREMNSEALKQTEKKRIPRAATSRAERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTEEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPERDENGVRPAIGQRTRLSKGDIAQARKLYRCPACGETLQDSTSNFTSPGFPNGYPSYTHCVWRISVTPGEKIVLNFTTMDLFKSRLCWYDYIEVRDGYWRKAPLLGRYCGDKLPEVLISTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKESGQIQSPNYPDDYRPMKECVWKITMSEHYHVGLIFQAFEIERHDNCAYDYLEVRDGSSENSPMIGRFCGYDKPEDIKSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECARPDNGGCEQRCLNTLGSYKCTCDPGYELAADKKSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISMQFEFFELEGNDICKYDYVEIRSGLSSDSKLHGKFCGTEVPEVITSRYNNMRIEFKSDNTVSKKGFKAHFLSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHQIHSATGVITSPNWPDKYPSRKECTWEILATPGHRVKITFNEFDIEQHQECAYDHLEVFDGESDKYPILGRFCGSKMPDPVLSTTNKMFIGFISDASVQRKGFQATHSTECGGRLKAEIKSKDLFSHAQFGDNNYPGQVDCEWQVLAEKGYSVELSFQTFEVEVEADCGYDFIELFDGFDTTASRLGRFCGSGPPEEIYSAGDAILIHFHTDDTINKKGFHIRYTSTKFQDAIQTKK

Target ORF information:

RefSeq Version	XM_007891468.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii tolloid-like 1 (tll1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007891468.1

ORF Insert Sequence:

ATGGGAACGG CAATAGAGCT GCTGCTCCTC TGTTGGGTGA GAAGAGTTGC AGATAGTGAG 
CTGAATAACA CAGATAGAGA AATGAACTCA GAAGCCTTAA AACAGACAGA GAAGAAAAGA 
ATTCCCCGAG CTGCAACTTC GCGAGCGGAG AGAATATGGC CTGGAGGTGT CATTCCATAT 
GTTATTGGAG GGAATTTTAC TGGCAGTCAG CGAGCTATGT TCAAACAGGC CATGCGGCAC 
TGGGAAAAGC ACACGTGTGT TACATTCATC GAACGAACAG AAGAAGAAAG TTACATTGTC 
TTCACGTACC GGCCATGTGG GTGCTGCTCC TATGTGGGTA GACGTGGAAA TGGTCCTCAA 
GCTATATCCA TAGGCAAAAA CTGTGACAAG TTTGGGATTG TGGTCCATGA ATTAGGGCAT 
GTGATTGGGT TTTGGCATGA ACACACAAGA CCAGACCGAG ATGATCATGT AACTATCATT 
CGAGAAAACA TCCAGCCTGG CCAGGAGTAC AACTTCCTCA AGATGGAACC TGGTGAAGTG 
AACTCCCTCG GGGAGTCATA CGACTTTGAC AGTATTATGC ACTACGCTAG GAACACCTTC 
TCAAGAGGAA TGTTTCTGGA CACCATTCTG CCTGAACGTG ACGAAAATGG TGTAAGACCT 
GCCATTGGCC AACGTACACG TTTAAGTAAA GGAGACATTG CACAGGCAAG GAAGCTGTAT 
AGATGTCCAG CGTGTGGGGA AACACTCCAA GACTCGACCA GTAATTTCAC GTCACCAGGA 
TTTCCGAATG GATACCCCTC TTACACACAT TGTGTTTGGA GAATCTCTGT TACACCTGGT 
GAAAAGATTG TGTTAAATTT TACCACCATG GATCTTTTCA AGAGCCGCCT GTGCTGGTAT 
GACTACATAG AAGTACGAGA TGGCTATTGG AGAAAGGCAC CGCTTCTTGG CAGATACTGT 
GGTGATAAAT TGCCTGAAGT CCTGATTTCC ACTGACAGTA GAATGTGGAT AGAATTTCGA 
AGCAGCAGTA ACTGGGTGGG AAAAGGTTTT GCAGCAGTCT ATGAAGCAAT CTGTGGAGGT 
GAGATCCACA AAGAATCCGG GCAGATTCAG TCTCCTAACT ATCCAGATGA CTACAGACCG 
ATGAAGGAGT GTGTATGGAA GATCACCATG TCTGAGCATT ACCATGTAGG ATTAATTTTC 
CAGGCATTTG AGATTGAGAG ACATGACAAT TGTGCCTATG ACTATCTAGA AGTACGAGAT 
GGCAGCAGTG AAAATAGTCC TATGATTGGG CGCTTCTGTG GCTACGACAA ACCAGAGGAC 
ATAAAATCCA CGTCTAATAC CTTGTGGATG AAGTTTGTTT CCGATGGAAC AGTTAATAAA 
GCAGGATTTG CTGCAAATTT CTTTAAAGAG GAGGATGAAT GTGCCAGGCC TGACAACGGA 
GGTTGCGAGC AACGATGTTT GAACACTTTG GGCAGTTATA AATGCACTTG CGATCCAGGC 
TACGAGCTTG CAGCAGACAA GAAGAGCTGT GAAGCTGCAT GCGGAGGACT CCTAACCAAG 
TTAAATGGAA CGATCACCAC ACCAGGCTGG CCTAAAGAAT ACCCTCCAAA TAAAAACTGT 
GTGTGGCAGG TTGTGGCTCC GACCCAATAC AGAATCTCGA TGCAGTTTGA ATTTTTTGAA 
TTAGAAGGCA ATGACATATG CAAGTACGAT TATGTGGAGA TCCGGAGTGG CCTTTCATCC 
GATTCCAAGC TTCATGGCAA GTTTTGCGGT ACGGAAGTGC CTGAAGTTAT AACATCCCGA 
TACAACAACA TGAGGATCGA GTTCAAATCA GACAATACTG TTTCAAAAAA AGGATTCAAA 
GCCCACTTTC TTTCAGACAA GGATGAGTGC TCCAAGGACA ACGGTGGCTG CCAACACGAG 
TGCATCAACA CCATAGGAAG TTATGTTTGC CAGTGTCGTA ATGGATTTGT GCTACATGAA 
AACAAACACG ACTGCAAAGA GGCTGAATGT GAGCACCAGA TCCACAGTGC TACAGGGGTC 
ATTACCAGCC CCAACTGGCC CGATAAATAC CCCAGCCGCA AAGAATGCAC GTGGGAGATC 
TTAGCTACCC CAGGGCACAG GGTGAAAATA ACTTTCAACG AGTTTGACAT TGAACAACAC 
CAGGAATGTG CTTACGATCA TCTGGAAGTG TTTGACGGAG AGTCTGATAA ATATCCAATT 
CTGGGGCGAT TCTGTGGCAG CAAAATGCCA GATCCAGTCC TCTCGACCAC AAACAAAATG 
TTCATTGGAT TCATTTCGGA TGCATCGGTA CAAAGGAAAG GTTTCCAGGC TACCCACTCC 
ACAGAATGTG GTGGCAGACT GAAGGCTGAA ATCAAATCTA AGGATTTGTT TTCTCACGCT 
CAGTTTGGAG ATAACAACTA CCCAGGCCAA GTGGACTGTG AGTGGCAGGT TTTAGCAGAA 
AAAGGCTACA GCGTGGAGCT CTCTTTTCAG ACGTTTGAAG TGGAGGTGGA AGCTGACTGT 
GGCTATGACT TTATTGAACT GTTCGATGGG TTTGATACAA CAGCTTCAAG ACTTGGGCGG 
TTCTGTGGAT CTGGACCACC CGAGGAAATC TATTCAGCGG GAGATGCCAT TTTAATCCAT 
TTCCATACTG ATGACACAAT AAACAAAAAA GGATTCCATA TACGATATAC AAGTACAAAA 
TTCCAGGATG CCATACAGAC CAAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tll1 ( XM_007891466.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007889657.1 Callorhinchus milii tolloid-like 1 (tll1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa374746
Clone ID Related Accession (Same CDS sequence)	XM_007891466.1
Accession Version	XM_007891466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA TTGCACTGTT ACTCACCTCC TTGACTCTCT CTGGGAAAAC GCAGCGAGTG 
AGTGGACTCC GGGACGATTA CACGTCCAAC TACTACGATG AAGAGAAAGG CGAGGTCATC 
GATTACAGAG ACCCTTGCAA AGCTGTTGCG TTTTGGGGCG ATATTGCCTT AGATGAAGAA 
GACTTGGAAC TCTTTCAGAT TGACAGGACA ATTGATTTAT CAAGACACAC TTTGGACAGA 
ATTGGACACA CAACAGGTGG ATTAGGAGAA CATAGAGTCT ATAAGAAAGG AGCTGTTTAT 
GAACTTATCG ATAGGATACG AAGATTGGGA GCAGTTGCAG ATAGTGAGCT GAATAACACA 
GATAGAGAAA TGAACTCAGA AGCCTTAAAA CAGACAGAGA AGAAAAGAAT TCCCCGAGCT 
GCAACTTCGC GAGCGGAGAG AATATGGCCT GGAGGTGTCA TTCCATATGT TATTGGAGGG 
AATTTTACTG GCAGTCAGCG AGCTATGTTC AAACAGGCCA TGCGGCACTG GGAAAAGCAC 
ACGTGTGTTA CATTCATCGA ACGAACAGAA GAAGAAAGTT ACATTGTCTT CACGTACCGG 
CCATGTGGGT GCTGCTCCTA TGTGGGTAGA CGTGGAAATG GTCCTCAAGC TATATCCATA 
GGCAAAAACT GTGACAAGTT TGGGATTGTG GTCCATGAAT TAGGGCATGT GATTGGGTTT 
TGGCATGAAC ACACAAGACC AGACCGAGAT GATCATGTAA CTATCATTCG AGAAAACATC 
CAGCCTGGCC AGGAGTACAA CTTCCTCAAG ATGGAACCTG GTGAAGTGAA CTCCCTCGGG 
GAGTCATACG ACTTTGACAG TATTATGCAC TACGCTAGGA ACACCTTCTC AAGAGGAATG 
TTTCTGGACA CCATTCTGCC TGAACGTGAC GAAAATGGTG TAAGACCTGC CATTGGCCAA 
CGTACACGTT TAAGTAAAGG AGACATTGCA CAGGCAAGGA AGCTGTATAG ATGTCCAGCG 
TGTGGGGAAA CACTCCAAGA CTCGACCAGT AATTTCACGT CACCAGGATT TCCGAATGGA 
TACCCCTCTT ACACACATTG TGTTTGGAGA ATCTCTGTTA CACCTGGTGA AAAGATTGTG 
TTAAATTTTA CCACCATGGA TCTTTTCAAG AGCCGCCTGT GCTGGTATGA CTACATAGAA 
GTACGAGATG GCTATTGGAG AAAGGCACCG CTTCTTGGCA GATACTGTGG TGATAAATTG 
CCTGAAGTCC TGATTTCCAC TGACAGTAGA ATGTGGATAG AATTTCGAAG CAGCAGTAAC 
TGGGTGGGAA AAGGTTTTGC AGCAGTCTAT GAAGCAATCT GTGGAGGTGA GATCCACAAA 
GAATCCGGGC AGATTCAGTC TCCTAACTAT CCAGATGACT ACAGACCGAT GAAGGAGTGT 
GTATGGAAGA TCACCATGTC TGAGCATTAC CATGTAGGAT TAATTTTCCA GGCATTTGAG 
ATTGAGAGAC ATGACAATTG TGCCTATGAC TATCTAGAAG TACGAGATGG CAGCAGTGAA 
AATAGTCCTA TGATTGGGCG CTTCTGTGGC TACGACAAAC CAGAGGACAT AAAATCCACG 
TCTAATACCT TGTGGATGAA GTTTGTTTCC GATGGAACAG TTAATAAAGC AGGATTTGCT 
GCAAATTTCT TTAAAGAGGA GGATGAATGT GCCAGGCCTG ACAACGGAGG TTGCGAGCAA 
CGATGTTTGA ACACTTTGGG CAGTTATAAA TGCACTTGCG ATCCAGGCTA CGAGCTTGCA 
GCAGACAAGA AGAGCTGTGA AGCTGCATGC GGAGGACTCC TAACCAAGTT AAATGGAACG 
ATCACCACAC CAGGCTGGCC TAAAGAATAC CCTCCAAATA AAAACTGTGT GTGGCAGGTT 
GTGGCTCCGA CCCAATACAG AATCTCGATG CAGTTTGAAT TTTTTGAATT AGAAGGCAAT 
GACATATGCA AGTACGATTA TGTGGAGATC CGGAGTGGCC TTTCATCCGA TTCCAAGCTT 
CATGGCAAGT TTTGCGGTAC GGAAGTGCCT GAAGTTATAA CATCCCGATA CAACAACATG 
AGGATCGAGT TCAAATCAGA CAATACTGTT TCAAAAAAAG GATTCAAAGC CCACTTTCTT 
TCAGACAAGG ATGAGTGCTC CAAGGACAAC GGTGGCTGCC AACACGAGTG CATCAACACC 
ATAGGAAGTT ATGTTTGCCA GTGTCGTAAT GGATTTGTGC TACATGAAAA CAAACACGAC 
TGCAAAGAGG CTGAATGTGA GCACCAGATC CACAGTGCTA CAGGGGTCAT TACCAGCCCC 
AACTGGCCCG ATAAATACCC CAGCCGCAAA GAATGCACGT GGGAGATCTT AGCTACCCCA 
GGGCACAGGG TGAAAATAAC TTTCAACGAG TTTGACATTG AACAACACCA GGAATGTGCT 
TACGATCATC TGGAAGTGTT TGACGGAGAG TCTGATAAAT ATCCAATTCT GGGGCGATTC 
TGTGGCAGCA AAATGCCAGA TCCAGTCCTC TCGACCACAA ACAAAATGTT CATTGGATTC 
ATTTCGGATG CATCGGTACA AAGGAAAGGT TTCCAGGCTA CCCACTCCAC AGAATGTGGT 
GGCAGACTGA AGGCTGAAAT CAAATCTAAG GATTTGTTTT CTCACGCTCA GTTTGGAGAT 
AACAACTACC CAGGCCAAGT GGACTGTGAG TGGCAGGTTT TAGCAGAAAA AGGCTACAGC 
GTGGAGCTCT CTTTTCAGAC GTTTGAAGTG GAGGTGGAAG CTGACTGTGG CTATGACTTT 
ATTGAACTGT TCGATGGGTT TGATACAACA GCTTCAAGAC TTGGGCGGTT CTGTGGATCT 
GGACCACCCG AGGAAATCTA TTCAGCGGGA GATGCCATTT TAATCCATTT CCATACTGAT 
GACACAATAA ACAAAAAAGG ATTCCATATA CGATATACAA GTACAAAATT CCAGGATGCC 
ATACAGACCA AAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007889657.1
CDS	75..3092
Translation	MNVIALLLTSLTLSGKTQRVSGLRDDYTSNYYDEEKGEVIDYRDPCKAVAFWGDIALDEEDLELFQIDRTIDLSRHTLDRIGHTTGGLGEHRVYKKGAVYELIDRIRRLGAVADSELNNTDREMNSEALKQTEKKRIPRAATSRAERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTEEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPERDENGVRPAIGQRTRLSKGDIAQARKLYRCPACGETLQDSTSNFTSPGFPNGYPSYTHCVWRISVTPGEKIVLNFTTMDLFKSRLCWYDYIEVRDGYWRKAPLLGRYCGDKLPEVLISTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKESGQIQSPNYPDDYRPMKECVWKITMSEHYHVGLIFQAFEIERHDNCAYDYLEVRDGSSENSPMIGRFCGYDKPEDIKSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECARPDNGGCEQRCLNTLGSYKCTCDPGYELAADKKSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISMQFEFFELEGNDICKYDYVEIRSGLSSDSKLHGKFCGTEVPEVITSRYNNMRIEFKSDNTVSKKGFKAHFLSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHQIHSATGVITSPNWPDKYPSRKECTWEILATPGHRVKITFNEFDIEQHQECAYDHLEVFDGESDKYPILGRFCGSKMPDPVLSTTNKMFIGFISDASVQRKGFQATHSTECGGRLKAEIKSKDLFSHAQFGDNNYPGQVDCEWQVLAEKGYSVELSFQTFEVEVEADCGYDFIELFDGFDTTASRLGRFCGSGPPEEIYSAGDAILIHFHTDDTINKKGFHIRYTSTKFQDAIQTKK

Target ORF information:

RefSeq Version	XM_007891466.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii tolloid-like 1 (tll1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007891466.1

ORF Insert Sequence:

ATGAATGTGA TTGCACTGTT ACTCACCTCC TTGACTCTCT CTGGGAAAAC GCAGCGAGTG 
AGTGGACTCC GGGACGATTA CACGTCCAAC TACTACGATG AAGAGAAAGG CGAGGTCATC 
GATTACAGAG ACCCTTGCAA AGCTGTTGCG TTTTGGGGCG ATATTGCCTT AGATGAAGAA 
GACTTGGAAC TCTTTCAGAT TGACAGGACA ATTGATTTAT CAAGACACAC TTTGGACAGA 
ATTGGACACA CAACAGGTGG ATTAGGAGAA CATAGAGTCT ATAAGAAAGG AGCTGTTTAT 
GAACTTATCG ATAGGATACG AAGATTGGGA GCAGTTGCAG ATAGTGAGCT GAATAACACA 
GATAGAGAAA TGAACTCAGA AGCCTTAAAA CAGACAGAGA AGAAAAGAAT TCCCCGAGCT 
GCAACTTCGC GAGCGGAGAG AATATGGCCT GGAGGTGTCA TTCCATATGT TATTGGAGGG 
AATTTTACTG GCAGTCAGCG AGCTATGTTC AAACAGGCCA TGCGGCACTG GGAAAAGCAC 
ACGTGTGTTA CATTCATCGA ACGAACAGAA GAAGAAAGTT ACATTGTCTT CACGTACCGG 
CCATGTGGGT GCTGCTCCTA TGTGGGTAGA CGTGGAAATG GTCCTCAAGC TATATCCATA 
GGCAAAAACT GTGACAAGTT TGGGATTGTG GTCCATGAAT TAGGGCATGT GATTGGGTTT 
TGGCATGAAC ACACAAGACC AGACCGAGAT GATCATGTAA CTATCATTCG AGAAAACATC 
CAGCCTGGCC AGGAGTACAA CTTCCTCAAG ATGGAACCTG GTGAAGTGAA CTCCCTCGGG 
GAGTCATACG ACTTTGACAG TATTATGCAC TACGCTAGGA ACACCTTCTC AAGAGGAATG 
TTTCTGGACA CCATTCTGCC TGAACGTGAC GAAAATGGTG TAAGACCTGC CATTGGCCAA 
CGTACACGTT TAAGTAAAGG AGACATTGCA CAGGCAAGGA AGCTGTATAG ATGTCCAGCG 
TGTGGGGAAA CACTCCAAGA CTCGACCAGT AATTTCACGT CACCAGGATT TCCGAATGGA 
TACCCCTCTT ACACACATTG TGTTTGGAGA ATCTCTGTTA CACCTGGTGA AAAGATTGTG 
TTAAATTTTA CCACCATGGA TCTTTTCAAG AGCCGCCTGT GCTGGTATGA CTACATAGAA 
GTACGAGATG GCTATTGGAG AAAGGCACCG CTTCTTGGCA GATACTGTGG TGATAAATTG 
CCTGAAGTCC TGATTTCCAC TGACAGTAGA ATGTGGATAG AATTTCGAAG CAGCAGTAAC 
TGGGTGGGAA AAGGTTTTGC AGCAGTCTAT GAAGCAATCT GTGGAGGTGA GATCCACAAA 
GAATCCGGGC AGATTCAGTC TCCTAACTAT CCAGATGACT ACAGACCGAT GAAGGAGTGT 
GTATGGAAGA TCACCATGTC TGAGCATTAC CATGTAGGAT TAATTTTCCA GGCATTTGAG 
ATTGAGAGAC ATGACAATTG TGCCTATGAC TATCTAGAAG TACGAGATGG CAGCAGTGAA 
AATAGTCCTA TGATTGGGCG CTTCTGTGGC TACGACAAAC CAGAGGACAT AAAATCCACG 
TCTAATACCT TGTGGATGAA GTTTGTTTCC GATGGAACAG TTAATAAAGC AGGATTTGCT 
GCAAATTTCT TTAAAGAGGA GGATGAATGT GCCAGGCCTG ACAACGGAGG TTGCGAGCAA 
CGATGTTTGA ACACTTTGGG CAGTTATAAA TGCACTTGCG ATCCAGGCTA CGAGCTTGCA 
GCAGACAAGA AGAGCTGTGA AGCTGCATGC GGAGGACTCC TAACCAAGTT AAATGGAACG 
ATCACCACAC CAGGCTGGCC TAAAGAATAC CCTCCAAATA AAAACTGTGT GTGGCAGGTT 
GTGGCTCCGA CCCAATACAG AATCTCGATG CAGTTTGAAT TTTTTGAATT AGAAGGCAAT 
GACATATGCA AGTACGATTA TGTGGAGATC CGGAGTGGCC TTTCATCCGA TTCCAAGCTT 
CATGGCAAGT TTTGCGGTAC GGAAGTGCCT GAAGTTATAA CATCCCGATA CAACAACATG 
AGGATCGAGT TCAAATCAGA CAATACTGTT TCAAAAAAAG GATTCAAAGC CCACTTTCTT 
TCAGACAAGG ATGAGTGCTC CAAGGACAAC GGTGGCTGCC AACACGAGTG CATCAACACC 
ATAGGAAGTT ATGTTTGCCA GTGTCGTAAT GGATTTGTGC TACATGAAAA CAAACACGAC 
TGCAAAGAGG CTGAATGTGA GCACCAGATC CACAGTGCTA CAGGGGTCAT TACCAGCCCC 
AACTGGCCCG ATAAATACCC CAGCCGCAAA GAATGCACGT GGGAGATCTT AGCTACCCCA 
GGGCACAGGG TGAAAATAAC TTTCAACGAG TTTGACATTG AACAACACCA GGAATGTGCT 
TACGATCATC TGGAAGTGTT TGACGGAGAG TCTGATAAAT ATCCAATTCT GGGGCGATTC 
TGTGGCAGCA AAATGCCAGA TCCAGTCCTC TCGACCACAA ACAAAATGTT CATTGGATTC 
ATTTCGGATG CATCGGTACA AAGGAAAGGT TTCCAGGCTA CCCACTCCAC AGAATGTGGT 
GGCAGACTGA AGGCTGAAAT CAAATCTAAG GATTTGTTTT CTCACGCTCA GTTTGGAGAT 
AACAACTACC CAGGCCAAGT GGACTGTGAG TGGCAGGTTT TAGCAGAAAA AGGCTACAGC 
GTGGAGCTCT CTTTTCAGAC GTTTGAAGTG GAGGTGGAAG CTGACTGTGG CTATGACTTT 
ATTGAACTGT TCGATGGGTT TGATACAACA GCTTCAAGAC TTGGGCGGTT CTGTGGATCT 
GGACCACCCG AGGAAATCTA TTCAGCGGGA GATGCCATTT TAATCCATTT CCATACTGAT 
GACACAATAA ACAAAAAAGG ATTCCATATA CGATATACAA GTACAAAATT CCAGGATGCC 
ATACAGACCA AAAAGTAA