slc12a4 cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a4
Entrez Gene ID	103176105
Full Name	solute carrier family 12 member 4
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007889491.1	XP_007887682.1	solute carrier family 12 member 4 isoform X2
XM_007889490.1	XP_007887681.1	solute carrier family 12 member 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following slc12a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa373292	XM_007889490.1 Latest version!	Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa373293	XM_007889491.1 Latest version!	Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a4 ( XM_007889490.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007887681.1 Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa373292
Clone ID Related Accession (Same CDS sequence)	XM_007889490.1
Accession Version	XM_007889490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	solute carrier family 12 member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA GCTTCCTCAT GACTTTCATC AAAGGGAAAT GCGAAGGGAG GCCGAGGAAA 
TCCGACACCG TGATCGAGAA GGAAGAGGAG GAGGAGGAAA AGAAGCGCGA GGAAACGAAA 
ACTAAGGAAA AGGGAAGCCA GGGGACCTCC GGCGCCCCGC CCCAGCCTGG GCCAGAAAAC 
CACAAAGAAA ACAGCCCTTT CCTAAACAAC ACGGATACTG TAAAAGGAAA TGAATACTAC 
GACAGAAATC TGGCCTTATT TGAGGAGGAA CTGGACATTC GACCAAAGGT GTCTTCCCTC 
CTCAACAGGT TGGCCAATTA TACAAACATC ACTCAGGGAG CAAAGGAACA TGAGGAATCA 
GAGAGTGGAG ACAATGCCAA GAAGAAAGTA TTTAAGTCTC CCAGTATGGG AACCCTGATG 
GGAGTTTACC TACCCTGCCT ACAAAATATT TTTGGAGTGA TTCTTTTTCT CCGACTGACA 
TGGCTTGTAG GAGCGGCAGG AGTCTTGCAG TCTTTCTGTA TTGTCTTCAT GTGCTGCTGC 
TGTACCATGC TAACAGCTAT ATCAATGAGT GCTATCGCAA CAAATGGAGT GGTACCAGCT 
GGAGGCTCTT ATTTCATGAT TTCAAGGTCA CTTGGTCCTG AATTCGGTGG TGCTGTTGGT 
CTGTGCTTTT ATCTTGGAAC TACTTTTGCA GGAGCCATGT ATATACTTGG TGCTATTGAA 
ATCTTATTGA CCTACATTGT CCCTGCAGCA GCGGTATTCA AACCTGTTGA CTCAAATGAG 
TCTGGTGCTA TGCTGAACAA CATGAGACTC TATGGGACAA TTTTCCTTGC TTTTATGGGC 
TTAGTTGTCT TCATAGGAGT CCGATATGTT AATAAATTTG CCTCCTTATT TTTGGTCTGT 
GTGATAATAT CCATCTTGTC CATTTATGTG GGTGTCATTA AAACAGCAGT CCATCCTCCA 
GAATTTAAAA TTTGTATGTT AGGAAATCGA ACACTTTCCA GAGGTTTGTT TGATGTTTGT 
GCCAAGACCA CTATTGAAAA CAATGAAACC GTGGCAACCC AGCTGTGGGA ATTTTTCTGC 
AACAGTAAAA ATCTAACCAC GGATGATTGT GACGAGTATT TTGTTCACAA TAATGTGTCT 
GAAATCCAAG GGATTCCTGG TGTGGCAAGT GGCATTATAT ACGAAAACCT GTGGAGCAGG 
TACATGACCA AGGGGGAGAT ACTCGAAAAG CAAACGTTGC AATCGGTTGA AACTTCTGGG 
CCAAAAAAGT ATATACCCAT CTATGTTCTT GCAGACATAA CCACTACTTT TACCATGCTG 
GTTGGCATCT TCTTTCCTTC AGTAACTGGT ATCATGGCTG GTTCAAATCG ATCTGGAGAC 
CTGAAGGATG CACAAAAGTC GATTCCTGTT GGTACAATAT TGGCTATTGC TACAACTTCA 
CTAGTTTACC TGAGTAGTGT GGTGTTATTT GGCGCCTGTG TCGAAGGTGT TGTCCTTCGC 
GATAAATACG GTGATGCTGT TAATAAGAAT TTAGTTGTGG GAACTCTAGC TTGGCCTTCT 
CCCTGGGTTA TTGTGGTTGG TTCCTTTTTC TCTACATGTG GAGCCGGTTT ACAAAGCTTA 
ACTGGGGCTC CCCGATTGCT ACAAGCAATT GCTAAGGACA ATATCATTCC TTTCTTACGG 
GTGTTTGGAC ATGGAAAAGC AAATGGTGAA CCAACTTGGG CTCTTCTTCT GACGGCTATA 
ATAGCTGAAC TCGGAATACT GATCGCCTCA CTTGATATGG TGGCTCCCAT TCTCTCAATG 
TTTTTCTTAA TGTGCTATTT GTTTGTTAAC CTGGCTTGTG CAGTACAAAC ACTTCTGCGA 
ACTCCCAACT GGCGTCCCAG ATTTAAATAC TACCATTGGG CTTTATCATT TTTAGGCATG 
AGCACATGCC TTGCTCTAAT GTTCATTTCT TCATGGTATT ACGCTCTTGT CGCTATGCTT 
ATCGCAGGCA TGATTTACAA GTATATTGAG TACCAAGGTG CAGAAAAAGA GTGGGGTGAT 
GGTATTCGGG GTCTGTCTCT GAGCGCAGCT CGTTATGCTT TGCTGAGGCT GGAAGAAGGT 
CCTCCTCATA CTAAGAACTG GAGGCCTCAG TTGCTGGTAC TGCTGAAACT AGATGAAGAT 
TTACATGTGA AACATCCCAG ATTGCTAACA TTTGCATCTC AGCTAAAAGC TGGCAAAGGT 
TTAACTATCG TTGGCTCAGT CATTCAAGGG AATTTTCTAG AAAACTACGG AGAAGCACAA 
GCCGCTGAAC AAACAATTAA AAGCATGATG GCCATAGAGA AGGTGAAAGG GTTTACTCAA 
TTGGTGGTTT CTTCCAAGGT TCGAGAAGGC ATTGCACATT TAATTCAATC TTGTGGACTT 
GGTGGAATGA AACACAATTC AGTAGTTATG GGTTGGCCTT ATGGCTGGAG ACAGAGTGAG 
GATCCCAGAG CCTGGAAAAC TTTCATTGAC ACTGTAAGAG TCACCACAGC TGGTCACCTT 
GCTCTGTTGG TGCCCAAGAA CGTCTCCCTC TATCCAAGTA ATCATGAACG CTTTACTGAG 
GGCCGCATTG ATGTTTGGTG GATTGTGCAC GATGGGGGCA TGTTGATGCT GCTCCCTTTC 
CTCCTCCGAC AACACAAGGT CTGGAAAAAA TGCACGATGC GCATTTTCAC TGTTGCACAA 
ATGGATGATA ACAGCATTCA GATGAAAAAG GACCTTGCAA CTTTCCTTTA CCACCTCCGA 
ATTGAGGCAG AAGTTGAAGT AGTTGAGATG CACGGCAGTG ATATTTCAGC TTATACATAC 
GAAAGAACTC TGATGATGGA GCAGCGGTCA CAAATGTTGA GGCAGATGAG GCTATCCAAG 
ACTGAGAGGG AAAGAGAGGC TCAGTTGGTA AAAGACAGGA ATTCAATGAT TCGTCAAGAA 
AGCATATACT CTGATGAAGA GGAAGAGCTA CATGTTTCTC CTGACAAAGT ACAGATGACC 
TGGACAAAAG AGAAATATGA ATCTGAGCGA AACCGTTGTA AGATGCATAC CCCGAACAGC 
TTCAGGGAAT TGATCAGTAT TAAACCAGAC CAGTCCAATG TAAGGAGAAT GCATACAGCG 
GTCAAGCTAA ATGAAGTCAT TGTAAATAGG TCTCATGATG CCAAGCTGGT TTTGCTGAAC 
ATGCCTGGAC CACCAAAGAA CCAGGATGGC GATGAAAACT ACATGGAATT TTTGGAAGTA 
CTTACAGAAG GTTTGGAGAG AGTACTTCTG GTCCGAGGTG GTGGTTGTGA AGTCATCACT 
ATTTATTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007887681.1
CDS	30..3341
Translation	MSESFLMTFIKGKCEGRPRKSDTVIEKEEEEEEKKREETKTKEKGSQGTSGAPPQPGPENHKENSPFLNNTDTVKGNEYYDRNLALFEEELDIRPKVSSLLNRLANYTNITQGAKEHEESESGDNAKKKVFKSPSMGTLMGVYLPCLQNIFGVILFLRLTWLVGAAGVLQSFCIVFMCCCCTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGAIEILLTYIVPAAAVFKPVDSNESGAMLNNMRLYGTIFLAFMGLVVFIGVRYVNKFASLFLVCVIISILSIYVGVIKTAVHPPEFKICMLGNRTLSRGLFDVCAKTTIENNETVATQLWEFFCNSKNLTTDDCDEYFVHNNVSEIQGIPGVASGIIYENLWSRYMTKGEILEKQTLQSVETSGPKKYIPIYVLADITTTFTMLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPVGTILAIATTSLVYLSSVVLFGACVEGVVLRDKYGDAVNKNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTAIIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSTCLALMFISSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIQGNFLENYGEAQAAEQTIKSMMAIEKVKGFTQLVVSSKVREGIAHLIQSCGLGGMKHNSVVMGWPYGWRQSEDPRAWKTFIDTVRVTTAGHLALLVPKNVSLYPSNHERFTEGRIDVWWIVHDGGMLMLLPFLLRQHKVWKKCTMRIFTVAQMDDNSIQMKKDLATFLYHLRIEAEVEVVEMHGSDISAYTYERTLMMEQRSQMLRQMRLSKTEREREAQLVKDRNSMIRQESIYSDEEEELHVSPDKVQMTWTKEKYESERNRCKMHTPNSFRELISIKPDQSNVRRMHTAVKLNEVIVNRSHDAKLVLLNMPGPPKNQDGDENYMEFLEVLTEGLERVLLVRGGGCEVITIYS

Target ORF information:

RefSeq Version	XM_007889490.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007889490.1

ORF Insert Sequence:

ATGTCGGAGA GCTTCCTCAT GACTTTCATC AAAGGGAAAT GCGAAGGGAG GCCGAGGAAA 
TCCGACACCG TGATCGAGAA GGAAGAGGAG GAGGAGGAAA AGAAGCGCGA GGAAACGAAA 
ACTAAGGAAA AGGGAAGCCA GGGGACCTCC GGCGCCCCGC CCCAGCCTGG GCCAGAAAAC 
CACAAAGAAA ACAGCCCTTT CCTAAACAAC ACGGATACTG TAAAAGGAAA TGAATACTAC 
GACAGAAATC TGGCCTTATT TGAGGAGGAA CTGGACATTC GACCAAAGGT GTCTTCCCTC 
CTCAACAGGT TGGCCAATTA TACAAACATC ACTCAGGGAG CAAAGGAACA TGAGGAATCA 
GAGAGTGGAG ACAATGCCAA GAAGAAAGTA TTTAAGTCTC CCAGTATGGG AACCCTGATG 
GGAGTTTACC TACCCTGCCT ACAAAATATT TTTGGAGTGA TTCTTTTTCT CCGACTGACA 
TGGCTTGTAG GAGCGGCAGG AGTCTTGCAG TCTTTCTGTA TTGTCTTCAT GTGCTGCTGC 
TGTACCATGC TAACAGCTAT ATCAATGAGT GCTATCGCAA CAAATGGAGT GGTACCAGCT 
GGAGGCTCTT ATTTCATGAT TTCAAGGTCA CTTGGTCCTG AATTCGGTGG TGCTGTTGGT 
CTGTGCTTTT ATCTTGGAAC TACTTTTGCA GGAGCCATGT ATATACTTGG TGCTATTGAA 
ATCTTATTGA CCTACATTGT CCCTGCAGCA GCGGTATTCA AACCTGTTGA CTCAAATGAG 
TCTGGTGCTA TGCTGAACAA CATGAGACTC TATGGGACAA TTTTCCTTGC TTTTATGGGC 
TTAGTTGTCT TCATAGGAGT CCGATATGTT AATAAATTTG CCTCCTTATT TTTGGTCTGT 
GTGATAATAT CCATCTTGTC CATTTATGTG GGTGTCATTA AAACAGCAGT CCATCCTCCA 
GAATTTAAAA TTTGTATGTT AGGAAATCGA ACACTTTCCA GAGGTTTGTT TGATGTTTGT 
GCCAAGACCA CTATTGAAAA CAATGAAACC GTGGCAACCC AGCTGTGGGA ATTTTTCTGC 
AACAGTAAAA ATCTAACCAC GGATGATTGT GACGAGTATT TTGTTCACAA TAATGTGTCT 
GAAATCCAAG GGATTCCTGG TGTGGCAAGT GGCATTATAT ACGAAAACCT GTGGAGCAGG 
TACATGACCA AGGGGGAGAT ACTCGAAAAG CAAACGTTGC AATCGGTTGA AACTTCTGGG 
CCAAAAAAGT ATATACCCAT CTATGTTCTT GCAGACATAA CCACTACTTT TACCATGCTG 
GTTGGCATCT TCTTTCCTTC AGTAACTGGT ATCATGGCTG GTTCAAATCG ATCTGGAGAC 
CTGAAGGATG CACAAAAGTC GATTCCTGTT GGTACAATAT TGGCTATTGC TACAACTTCA 
CTAGTTTACC TGAGTAGTGT GGTGTTATTT GGCGCCTGTG TCGAAGGTGT TGTCCTTCGC 
GATAAATACG GTGATGCTGT TAATAAGAAT TTAGTTGTGG GAACTCTAGC TTGGCCTTCT 
CCCTGGGTTA TTGTGGTTGG TTCCTTTTTC TCTACATGTG GAGCCGGTTT ACAAAGCTTA 
ACTGGGGCTC CCCGATTGCT ACAAGCAATT GCTAAGGACA ATATCATTCC TTTCTTACGG 
GTGTTTGGAC ATGGAAAAGC AAATGGTGAA CCAACTTGGG CTCTTCTTCT GACGGCTATA 
ATAGCTGAAC TCGGAATACT GATCGCCTCA CTTGATATGG TGGCTCCCAT TCTCTCAATG 
TTTTTCTTAA TGTGCTATTT GTTTGTTAAC CTGGCTTGTG CAGTACAAAC ACTTCTGCGA 
ACTCCCAACT GGCGTCCCAG ATTTAAATAC TACCATTGGG CTTTATCATT TTTAGGCATG 
AGCACATGCC TTGCTCTAAT GTTCATTTCT TCATGGTATT ACGCTCTTGT CGCTATGCTT 
ATCGCAGGCA TGATTTACAA GTATATTGAG TACCAAGGTG CAGAAAAAGA GTGGGGTGAT 
GGTATTCGGG GTCTGTCTCT GAGCGCAGCT CGTTATGCTT TGCTGAGGCT GGAAGAAGGT 
CCTCCTCATA CTAAGAACTG GAGGCCTCAG TTGCTGGTAC TGCTGAAACT AGATGAAGAT 
TTACATGTGA AACATCCCAG ATTGCTAACA TTTGCATCTC AGCTAAAAGC TGGCAAAGGT 
TTAACTATCG TTGGCTCAGT CATTCAAGGG AATTTTCTAG AAAACTACGG AGAAGCACAA 
GCCGCTGAAC AAACAATTAA AAGCATGATG GCCATAGAGA AGGTGAAAGG GTTTACTCAA 
TTGGTGGTTT CTTCCAAGGT TCGAGAAGGC ATTGCACATT TAATTCAATC TTGTGGACTT 
GGTGGAATGA AACACAATTC AGTAGTTATG GGTTGGCCTT ATGGCTGGAG ACAGAGTGAG 
GATCCCAGAG CCTGGAAAAC TTTCATTGAC ACTGTAAGAG TCACCACAGC TGGTCACCTT 
GCTCTGTTGG TGCCCAAGAA CGTCTCCCTC TATCCAAGTA ATCATGAACG CTTTACTGAG 
GGCCGCATTG ATGTTTGGTG GATTGTGCAC GATGGGGGCA TGTTGATGCT GCTCCCTTTC 
CTCCTCCGAC AACACAAGGT CTGGAAAAAA TGCACGATGC GCATTTTCAC TGTTGCACAA 
ATGGATGATA ACAGCATTCA GATGAAAAAG GACCTTGCAA CTTTCCTTTA CCACCTCCGA 
ATTGAGGCAG AAGTTGAAGT AGTTGAGATG CACGGCAGTG ATATTTCAGC TTATACATAC 
GAAAGAACTC TGATGATGGA GCAGCGGTCA CAAATGTTGA GGCAGATGAG GCTATCCAAG 
ACTGAGAGGG AAAGAGAGGC TCAGTTGGTA AAAGACAGGA ATTCAATGAT TCGTCAAGAA 
AGCATATACT CTGATGAAGA GGAAGAGCTA CATGTTTCTC CTGACAAAGT ACAGATGACC 
TGGACAAAAG AGAAATATGA ATCTGAGCGA AACCGTTGTA AGATGCATAC CCCGAACAGC 
TTCAGGGAAT TGATCAGTAT TAAACCAGAC CAGTCCAATG TAAGGAGAAT GCATACAGCG 
GTCAAGCTAA ATGAAGTCAT TGTAAATAGG TCTCATGATG CCAAGCTGGT TTTGCTGAAC 
ATGCCTGGAC CACCAAAGAA CCAGGATGGC GATGAAAACT ACATGGAATT TTTGGAAGTA 
CTTACAGAAG GTTTGGAGAG AGTACTTCTG GTCCGAGGTG GTGGTTGTGA AGTCATCACT 
ATTTATTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a4 ( XM_007889491.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007887682.1 Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa373293
Clone ID Related Accession (Same CDS sequence)	XM_007889491.1
Accession Version	XM_007889491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	solute carrier family 12 member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TTACTGTGGT GCCAGTAGAA GACCAGAAAT CAGATTATGA TAGTTTGGAA 
GGAATCACCT GGGTTGATTA CAGAGAGCCT GGACAGAGTT CAGCAGACCC TGATGATACA 
GTGAGCTTTG ATGAAAACCA CAAAGAAAAC AGCCCTTTCC TAAACAACAC GGATACTGTA 
AAAGGAAATG AATACTACGA CAGAAATCTG GCCTTATTTG AGGAGGAACT GGACATTCGA 
CCAAAGGTGT CTTCCCTCCT CAACAGGTTG GCCAATTATA CAAACATCAC TCAGGGAGCA 
AAGGAACATG AGGAATCAGA GAGTGGAGAC AATGCCAAGA AGAAAGTATT TAAGTCTCCC 
AGTATGGGAA CCCTGATGGG AGTTTACCTA CCCTGCCTAC AAAATATTTT TGGAGTGATT 
CTTTTTCTCC GACTGACATG GCTTGTAGGA GCGGCAGGAG TCTTGCAGTC TTTCTGTATT 
GTCTTCATGT GCTGCTGCTG TACCATGCTA ACAGCTATAT CAATGAGTGC TATCGCAACA 
AATGGAGTGG TACCAGCTGG AGGCTCTTAT TTCATGATTT CAAGGTCACT TGGTCCTGAA 
TTCGGTGGTG CTGTTGGTCT GTGCTTTTAT CTTGGAACTA CTTTTGCAGG AGCCATGTAT 
ATACTTGGTG CTATTGAAAT CTTATTGACC TACATTGTCC CTGCAGCAGC GGTATTCAAA 
CCTGTTGACT CAAATGAGTC TGGTGCTATG CTGAACAACA TGAGACTCTA TGGGACAATT 
TTCCTTGCTT TTATGGGCTT AGTTGTCTTC ATAGGAGTCC GATATGTTAA TAAATTTGCC 
TCCTTATTTT TGGTCTGTGT GATAATATCC ATCTTGTCCA TTTATGTGGG TGTCATTAAA 
ACAGCAGTCC ATCCTCCAGA ATTTAAAATT TGTATGTTAG GAAATCGAAC ACTTTCCAGA 
GGTTTGTTTG ATGTTTGTGC CAAGACCACT ATTGAAAACA ATGAAACCGT GGCAACCCAG 
CTGTGGGAAT TTTTCTGCAA CAGTAAAAAT CTAACCACGG ATGATTGTGA CGAGTATTTT 
GTTCACAATA ATGTGTCTGA AATCCAAGGG ATTCCTGGTG TGGCAAGTGG CATTATATAC 
GAAAACCTGT GGAGCAGGTA CATGACCAAG GGGGAGATAC TCGAAAAGCA AACGTTGCAA 
TCGGTTGAAA CTTCTGGGCC AAAAAAGTAT ATACCCATCT ATGTTCTTGC AGACATAACC 
ACTACTTTTA CCATGCTGGT TGGCATCTTC TTTCCTTCAG TAACTGGTAT CATGGCTGGT 
TCAAATCGAT CTGGAGACCT GAAGGATGCA CAAAAGTCGA TTCCTGTTGG TACAATATTG 
GCTATTGCTA CAACTTCACT AGTTTACCTG AGTAGTGTGG TGTTATTTGG CGCCTGTGTC 
GAAGGTGTTG TCCTTCGCGA TAAATACGGT GATGCTGTTA ATAAGAATTT AGTTGTGGGA 
ACTCTAGCTT GGCCTTCTCC CTGGGTTATT GTGGTTGGTT CCTTTTTCTC TACATGTGGA 
GCCGGTTTAC AAAGCTTAAC TGGGGCTCCC CGATTGCTAC AAGCAATTGC TAAGGACAAT 
ATCATTCCTT TCTTACGGGT GTTTGGACAT GGAAAAGCAA ATGGTGAACC AACTTGGGCT 
CTTCTTCTGA CGGCTATAAT AGCTGAACTC GGAATACTGA TCGCCTCACT TGATATGGTG 
GCTCCCATTC TCTCAATGTT TTTCTTAATG TGCTATTTGT TTGTTAACCT GGCTTGTGCA 
GTACAAACAC TTCTGCGAAC TCCCAACTGG CGTCCCAGAT TTAAATACTA CCATTGGGCT 
TTATCATTTT TAGGCATGAG CACATGCCTT GCTCTAATGT TCATTTCTTC ATGGTATTAC 
GCTCTTGTCG CTATGCTTAT CGCAGGCATG ATTTACAAGT ATATTGAGTA CCAAGGTGCA 
GAAAAAGAGT GGGGTGATGG TATTCGGGGT CTGTCTCTGA GCGCAGCTCG TTATGCTTTG 
CTGAGGCTGG AAGAAGGTCC TCCTCATACT AAGAACTGGA GGCCTCAGTT GCTGGTACTG 
CTGAAACTAG ATGAAGATTT ACATGTGAAA CATCCCAGAT TGCTAACATT TGCATCTCAG 
CTAAAAGCTG GCAAAGGTTT AACTATCGTT GGCTCAGTCA TTCAAGGGAA TTTTCTAGAA 
AACTACGGAG AAGCACAAGC CGCTGAACAA ACAATTAAAA GCATGATGGC CATAGAGAAG 
GTGAAAGGGT TTACTCAATT GGTGGTTTCT TCCAAGGTTC GAGAAGGCAT TGCACATTTA 
ATTCAATCTT GTGGACTTGG TGGAATGAAA CACAATTCAG TAGTTATGGG TTGGCCTTAT 
GGCTGGAGAC AGAGTGAGGA TCCCAGAGCC TGGAAAACTT TCATTGACAC TGTAAGAGTC 
ACCACAGCTG GTCACCTTGC TCTGTTGGTG CCCAAGAACG TCTCCCTCTA TCCAAGTAAT 
CATGAACGCT TTACTGAGGG CCGCATTGAT GTTTGGTGGA TTGTGCACGA TGGGGGCATG 
TTGATGCTGC TCCCTTTCCT CCTCCGACAA CACAAGGTCT GGAAAAAATG CACGATGCGC 
ATTTTCACTG TTGCACAAAT GGATGATAAC AGCATTCAGA TGAAAAAGGA CCTTGCAACT 
TTCCTTTACC ACCTCCGAAT TGAGGCAGAA GTTGAAGTAG TTGAGATGCA CGGCAGTGAT 
ATTTCAGCTT ATACATACGA AAGAACTCTG ATGATGGAGC AGCGGTCACA AATGTTGAGG 
CAGATGAGGC TATCCAAGAC TGAGAGGGAA AGAGAGGCTC AGTTGGTAAA AGACAGGAAT 
TCAATGATTC GTCAAGAAAG CATATACTCT GATGAAGAGG AAGAGCTACA TGTTTCTCCT 
GACAAAGTAC AGATGACCTG GACAAAAGAG AAATATGAAT CTGAGCGAAA CCGTTGTAAG 
ATGCATACCC CGAACAGCTT CAGGGAATTG ATCAGTATTA AACCAGACCA GTCCAATGTA 
AGGAGAATGC ATACAGCGGT CAAGCTAAAT GAAGTCATTG TAAATAGGTC TCATGATGCC 
AAGCTGGTTT TGCTGAACAT GCCTGGACCA CCAAAGAACC AGGATGGCGA TGAAAACTAC 
ATGGAATTTT TGGAAGTACT TACAGAAGGT TTGGAGAGAG TACTTCTGGT CCGAGGTGGT 
GGTTGTGAAG TCATCACTAT TTATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007887682.1
CDS	101..3370
Translation	MPHFTVVPVEDQKSDYDSLEGITWVDYREPGQSSADPDDTVSFDENHKENSPFLNNTDTVKGNEYYDRNLALFEEELDIRPKVSSLLNRLANYTNITQGAKEHEESESGDNAKKKVFKSPSMGTLMGVYLPCLQNIFGVILFLRLTWLVGAAGVLQSFCIVFMCCCCTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGAIEILLTYIVPAAAVFKPVDSNESGAMLNNMRLYGTIFLAFMGLVVFIGVRYVNKFASLFLVCVIISILSIYVGVIKTAVHPPEFKICMLGNRTLSRGLFDVCAKTTIENNETVATQLWEFFCNSKNLTTDDCDEYFVHNNVSEIQGIPGVASGIIYENLWSRYMTKGEILEKQTLQSVETSGPKKYIPIYVLADITTTFTMLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPVGTILAIATTSLVYLSSVVLFGACVEGVVLRDKYGDAVNKNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTAIIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSTCLALMFISSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIQGNFLENYGEAQAAEQTIKSMMAIEKVKGFTQLVVSSKVREGIAHLIQSCGLGGMKHNSVVMGWPYGWRQSEDPRAWKTFIDTVRVTTAGHLALLVPKNVSLYPSNHERFTEGRIDVWWIVHDGGMLMLLPFLLRQHKVWKKCTMRIFTVAQMDDNSIQMKKDLATFLYHLRIEAEVEVVEMHGSDISAYTYERTLMMEQRSQMLRQMRLSKTEREREAQLVKDRNSMIRQESIYSDEEEELHVSPDKVQMTWTKEKYESERNRCKMHTPNSFRELISIKPDQSNVRRMHTAVKLNEVIVNRSHDAKLVLLNMPGPPKNQDGDENYMEFLEVLTEGLERVLLVRGGGCEVITIYS

Target ORF information:

RefSeq Version	XM_007889491.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007889491.1

ORF Insert Sequence:

ATGCCTCATT TTACTGTGGT GCCAGTAGAA GACCAGAAAT CAGATTATGA TAGTTTGGAA 
GGAATCACCT GGGTTGATTA CAGAGAGCCT GGACAGAGTT CAGCAGACCC TGATGATACA 
GTGAGCTTTG ATGAAAACCA CAAAGAAAAC AGCCCTTTCC TAAACAACAC GGATACTGTA 
AAAGGAAATG AATACTACGA CAGAAATCTG GCCTTATTTG AGGAGGAACT GGACATTCGA 
CCAAAGGTGT CTTCCCTCCT CAACAGGTTG GCCAATTATA CAAACATCAC TCAGGGAGCA 
AAGGAACATG AGGAATCAGA GAGTGGAGAC AATGCCAAGA AGAAAGTATT TAAGTCTCCC 
AGTATGGGAA CCCTGATGGG AGTTTACCTA CCCTGCCTAC AAAATATTTT TGGAGTGATT 
CTTTTTCTCC GACTGACATG GCTTGTAGGA GCGGCAGGAG TCTTGCAGTC TTTCTGTATT 
GTCTTCATGT GCTGCTGCTG TACCATGCTA ACAGCTATAT CAATGAGTGC TATCGCAACA 
AATGGAGTGG TACCAGCTGG AGGCTCTTAT TTCATGATTT CAAGGTCACT TGGTCCTGAA 
TTCGGTGGTG CTGTTGGTCT GTGCTTTTAT CTTGGAACTA CTTTTGCAGG AGCCATGTAT 
ATACTTGGTG CTATTGAAAT CTTATTGACC TACATTGTCC CTGCAGCAGC GGTATTCAAA 
CCTGTTGACT CAAATGAGTC TGGTGCTATG CTGAACAACA TGAGACTCTA TGGGACAATT 
TTCCTTGCTT TTATGGGCTT AGTTGTCTTC ATAGGAGTCC GATATGTTAA TAAATTTGCC 
TCCTTATTTT TGGTCTGTGT GATAATATCC ATCTTGTCCA TTTATGTGGG TGTCATTAAA 
ACAGCAGTCC ATCCTCCAGA ATTTAAAATT TGTATGTTAG GAAATCGAAC ACTTTCCAGA 
GGTTTGTTTG ATGTTTGTGC CAAGACCACT ATTGAAAACA ATGAAACCGT GGCAACCCAG 
CTGTGGGAAT TTTTCTGCAA CAGTAAAAAT CTAACCACGG ATGATTGTGA CGAGTATTTT 
GTTCACAATA ATGTGTCTGA AATCCAAGGG ATTCCTGGTG TGGCAAGTGG CATTATATAC 
GAAAACCTGT GGAGCAGGTA CATGACCAAG GGGGAGATAC TCGAAAAGCA AACGTTGCAA 
TCGGTTGAAA CTTCTGGGCC AAAAAAGTAT ATACCCATCT ATGTTCTTGC AGACATAACC 
ACTACTTTTA CCATGCTGGT TGGCATCTTC TTTCCTTCAG TAACTGGTAT CATGGCTGGT 
TCAAATCGAT CTGGAGACCT GAAGGATGCA CAAAAGTCGA TTCCTGTTGG TACAATATTG 
GCTATTGCTA CAACTTCACT AGTTTACCTG AGTAGTGTGG TGTTATTTGG CGCCTGTGTC 
GAAGGTGTTG TCCTTCGCGA TAAATACGGT GATGCTGTTA ATAAGAATTT AGTTGTGGGA 
ACTCTAGCTT GGCCTTCTCC CTGGGTTATT GTGGTTGGTT CCTTTTTCTC TACATGTGGA 
GCCGGTTTAC AAAGCTTAAC TGGGGCTCCC CGATTGCTAC AAGCAATTGC TAAGGACAAT 
ATCATTCCTT TCTTACGGGT GTTTGGACAT GGAAAAGCAA ATGGTGAACC AACTTGGGCT 
CTTCTTCTGA CGGCTATAAT AGCTGAACTC GGAATACTGA TCGCCTCACT TGATATGGTG 
GCTCCCATTC TCTCAATGTT TTTCTTAATG TGCTATTTGT TTGTTAACCT GGCTTGTGCA 
GTACAAACAC TTCTGCGAAC TCCCAACTGG CGTCCCAGAT TTAAATACTA CCATTGGGCT 
TTATCATTTT TAGGCATGAG CACATGCCTT GCTCTAATGT TCATTTCTTC ATGGTATTAC 
GCTCTTGTCG CTATGCTTAT CGCAGGCATG ATTTACAAGT ATATTGAGTA CCAAGGTGCA 
GAAAAAGAGT GGGGTGATGG TATTCGGGGT CTGTCTCTGA GCGCAGCTCG TTATGCTTTG 
CTGAGGCTGG AAGAAGGTCC TCCTCATACT AAGAACTGGA GGCCTCAGTT GCTGGTACTG 
CTGAAACTAG ATGAAGATTT ACATGTGAAA CATCCCAGAT TGCTAACATT TGCATCTCAG 
CTAAAAGCTG GCAAAGGTTT AACTATCGTT GGCTCAGTCA TTCAAGGGAA TTTTCTAGAA 
AACTACGGAG AAGCACAAGC CGCTGAACAA ACAATTAAAA GCATGATGGC CATAGAGAAG 
GTGAAAGGGT TTACTCAATT GGTGGTTTCT TCCAAGGTTC GAGAAGGCAT TGCACATTTA 
ATTCAATCTT GTGGACTTGG TGGAATGAAA CACAATTCAG TAGTTATGGG TTGGCCTTAT 
GGCTGGAGAC AGAGTGAGGA TCCCAGAGCC TGGAAAACTT TCATTGACAC TGTAAGAGTC 
ACCACAGCTG GTCACCTTGC TCTGTTGGTG CCCAAGAACG TCTCCCTCTA TCCAAGTAAT 
CATGAACGCT TTACTGAGGG CCGCATTGAT GTTTGGTGGA TTGTGCACGA TGGGGGCATG 
TTGATGCTGC TCCCTTTCCT CCTCCGACAA CACAAGGTCT GGAAAAAATG CACGATGCGC 
ATTTTCACTG TTGCACAAAT GGATGATAAC AGCATTCAGA TGAAAAAGGA CCTTGCAACT 
TTCCTTTACC ACCTCCGAAT TGAGGCAGAA GTTGAAGTAG TTGAGATGCA CGGCAGTGAT 
ATTTCAGCTT ATACATACGA AAGAACTCTG ATGATGGAGC AGCGGTCACA AATGTTGAGG 
CAGATGAGGC TATCCAAGAC TGAGAGGGAA AGAGAGGCTC AGTTGGTAAA AGACAGGAAT 
TCAATGATTC GTCAAGAAAG CATATACTCT GATGAAGAGG AAGAGCTACA TGTTTCTCCT 
GACAAAGTAC AGATGACCTG GACAAAAGAG AAATATGAAT CTGAGCGAAA CCGTTGTAAG 
ATGCATACCC CGAACAGCTT CAGGGAATTG ATCAGTATTA AACCAGACCA GTCCAATGTA 
AGGAGAATGC ATACAGCGGT CAAGCTAAAT GAAGTCATTG TAAATAGGTC TCATGATGCC 
AAGCTGGTTT TGCTGAACAT GCCTGGACCA CCAAAGAACC AGGATGGCGA TGAAAACTAC 
ATGGAATTTT TGGAAGTACT TACAGAAGGT TTGGAGAGAG TACTTCTGGT CCGAGGTGGT 
GGTTGTGAAG TCATCACTAT TTATTCTTGA