CHUK cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHUK
Entrez Gene ID	106902832
Full Name	conserved helix-loop-helix ubiquitous kinase
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014966638.1	XP_014822124.1	inhibitor of nuclear factor kappa-B kinase subunit alpha

Pathways from BioSystems
Gene Ontology

The following CHUK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHUK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa60421	XM_014966638.1 Latest version!	Calidris pugnax conserved helix-loop-helix ubiquitous kinase (CHUK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHUK ( XM_014966638.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014822124.1 Calidris pugnax conserved helix-loop-helix ubiquitous kinase (CHUK), transcript variant X1, mRNA.

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CloneID	OCa60421
Clone ID Related Accession (Same CDS sequence)	XM_014966638.1
Accession Version	XM_014966638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGACC GCCTGGGGAC CGGCGGCTTC GGCAACGTCT GCCTCTACCA GCACCAGGAC 
TCGGGCGTCC GGGTGGCCAT CAAGTCCTGC CGTCTGGAGC TCAGCGTCAA GAACAAGGAC 
CGCTGGTGCC ACGAGATAGA CATCATGAAG AAGTTGAACC ATCCCAATGT AGTAAGAGCC 
TGTGAAGTCC CTGAGGAGAT GAACTTTCTT GTTAACGACG TTCCCCTTCT GGCAATGGAA 
TATTGTTCAG GGGGTGACCT CCGGAAGTTG CTAAACAAAC CAGAAAACTG CTGTGGACTT 
AAGGAAAGTC AAATACTTTC TCTGCTTAGT GACATTGGGT CTGGTATCCA ATATTTGCAC 
GAGAACAGAA TTATACACAG AGATTTAAAG CCTGAAAATA TTGTTCTTCA GGATGAAGGT 
GGGAAGATTG TTCACAAAAT AATAGACCTG GGATACGCAA AGGATCTGGA TCAGGGAAGT 
TTATGCACTT CATTTGTTGG AACATTACAA TATTTGGCTC CAGAACTCTT TGAGAATAAA 
TCATACTCAG TTACTGTTGA TTACTGGAGC TTTGGAACGA TGGTGTTTGA ATGCATTGCT 
GGATTTAGAC CTTTCTTACA TAATCTACAG CCATTTACCT GGCATGAAAA AATCAAGAAA 
AAAGATCCAA AGCATATCTT TGCTTCTGAA GAGATGAATG GGGAGGTTCA CTTTAGTACC 
CATTTGCCAG AGCCTCACAG CCTCTGTGGT GTAATTGTAG AACCGATGGA AAATTGGTTG 
CAACTGATGC TGAACTGGGA TCCGCAACAG AGGGGTGGAG GTTTAGATCC TGAGACTAGT 
CGTCCAAAGT GTTTCCTAAT AATGGACCAC ATACTGAATT TGAAGATAGT ACACATCCTA 
AGCATGACCT CTGCCAAGAT TATTTCATTT CTCTTGCATC CTGAGGAAAG CCTTCATTCC 
CTTCAGATTC GGATTGAGTT TGAAACTGGA ATAAGTACTG GAAATCAGGA GCTGCTGTTG 
GAAACGGGGA TTTGCCTGGA CCCTCGGAAA CCTGCTTCAC AGTGTGTTAT TGATGGCGTG 
AGAGGCTGGG ATAGCTACAT GGTCTATTTG TTTGATAAAA GCAAAACTGT CTATGATGGA 
CCCTTTGATT CTCGGAGTCT TTCTGACTGT GTAAATTACA TTGTACAGGA TAGTAAAATC 
CAACTGCCAA TTCCTCAGCT GCGCAAGGTA TGGGCAGAAG CAGTTCACTA CGTGATTGGG 
CTAAAAGAAG ATTATAGCAG GCTTTTCCAG GGCCAGAGAG CTGCCATGCT CAGTCTTCTT 
CGGTATAATG CCAACCTAAT CAAAATGAAA AACAATATGG TGTCAGCATC TCAGCAGCTG 
AAAGCAAAGC TGGAATTCTT TCATCAAAGC ATTCGCCTCG ACTTGGAAAG ATACAGCGAC 
CAGATGGCTT ATGGCATATC TTCAGAAAAG ATGCTCAAAG CCTGGAAGGA GATGCAAGAG 
AAGGCCTCTC AGTGTGCACA GGCTGAAGAT ATAGGATATC TGGATGAACA GATCATGGCT 
TTGCACACAG AGATCGTAGA GCTTCAGAAG AGTCCATATG CAAGGCGCCA AGGAGAGGTT 
ATGGAGAGCT TGGAACAGAG AGCCATAGAC CTGTACAAGC AATTAAAAAC AAGACCTCCA 
GATCATGCCT ACAGTGACAG CACGGACATG GTGAAGATCA TTGTGCAGAC AGTACAAAGC 
CAAGACCGAG TTCTCAAGGA GCTTTTCGGC CATCTCAGCA AGCTCTTGGG CTGTAAGCAG 
AAGATCATTG ACTTGCTTCC AAAGATTGAA GTGGCTCTAA ATAACATCAA GGAAGCTGAC 
AACTCAGTGA TGCAGATGCA GGGCAAAAGA CAAAGGGAGA TATGGCATTT GTTGAAAATT 
GCTTGTACGC AGAGCTCTTC TCGATCACTG GTAAGCTCCA GCCTGGAAGG GACAGCCTCA 
ACTCCAGCAG TCACATGGCT CCAACAGAGC TCCTCAGGGA ACATACCTCA CCCTCTGTCA 
TCTCTGGCAA CTCCTGAAGA CAGGGAAAAT TTTGTCCATG TGATAGAAGA GAACCTGAAT 
TACCTTGAAC TCTTTAGCAG TATTTTGCAG GAGGTGAGAC AGGAGCAGAA CAACAGCATG 
ATGAGTTTTG ATTGGAGCTG GCTGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014822124.1
CDS	60..2249
Translation	MRDRLGTGGFGNVCLYQHQDSGVRVAIKSCRLELSVKNKDRWCHEIDIMKKLNHPNVVRACEVPEEMNFLVNDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIQYLHENRIIHRDLKPENIVLQDEGGKIVHKIIDLGYAKDLDQGSLCTSFVGTLQYLAPELFENKSYSVTVDYWSFGTMVFECIAGFRPFLHNLQPFTWHEKIKKKDPKHIFASEEMNGEVHFSTHLPEPHSLCGVIVEPMENWLQLMLNWDPQQRGGGLDPETSRPKCFLIMDHILNLKIVHILSMTSAKIISFLLHPEESLHSLQIRIEFETGISTGNQELLLETGICLDPRKPASQCVIDGVRGWDSYMVYLFDKSKTVYDGPFDSRSLSDCVNYIVQDSKIQLPIPQLRKVWAEAVHYVIGLKEDYSRLFQGQRAAMLSLLRYNANLIKMKNNMVSASQQLKAKLEFFHQSIRLDLERYSDQMAYGISSEKMLKAWKEMQEKASQCAQAEDIGYLDEQIMALHTEIVELQKSPYARRQGEVMESLEQRAIDLYKQLKTRPPDHAYSDSTDMVKIIVQTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKIEVALNNIKEADNSVMQMQGKRQREIWHLLKIACTQSSSRSLVSSSLEGTASTPAVTWLQQSSSGNIPHPLSSLATPEDRENFVHVIEENLNYLELFSSILQEVRQEQNNSMMSFDWSWLK

Target ORF information:

RefSeq Version	XM_014966638.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax conserved helix-loop-helix ubiquitous kinase (CHUK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014966638.1

ORF Insert Sequence:

ATGCGGGACC GCCTGGGGAC CGGCGGCTTC GGCAACGTCT GCCTCTACCA GCACCAGGAC 
TCGGGCGTCC GGGTGGCCAT CAAGTCCTGC CGTCTGGAGC TCAGCGTCAA GAACAAGGAC 
CGCTGGTGCC ACGAGATAGA CATCATGAAG AAGTTGAACC ATCCCAATGT AGTAAGAGCC 
TGTGAAGTCC CTGAGGAGAT GAACTTTCTT GTTAACGACG TTCCCCTTCT GGCAATGGAA 
TATTGTTCAG GGGGTGACCT CCGGAAGTTG CTAAACAAAC CAGAAAACTG CTGTGGACTT 
AAGGAAAGTC AAATACTTTC TCTGCTTAGT GACATTGGGT CTGGTATCCA ATATTTGCAC 
GAGAACAGAA TTATACACAG AGATTTAAAG CCTGAAAATA TTGTTCTTCA GGATGAAGGT 
GGGAAGATTG TTCACAAAAT AATAGACCTG GGATACGCAA AGGATCTGGA TCAGGGAAGT 
TTATGCACTT CATTTGTTGG AACATTACAA TATTTGGCTC CAGAACTCTT TGAGAATAAA 
TCATACTCAG TTACTGTTGA TTACTGGAGC TTTGGAACGA TGGTGTTTGA ATGCATTGCT 
GGATTTAGAC CTTTCTTACA TAATCTACAG CCATTTACCT GGCATGAAAA AATCAAGAAA 
AAAGATCCAA AGCATATCTT TGCTTCTGAA GAGATGAATG GGGAGGTTCA CTTTAGTACC 
CATTTGCCAG AGCCTCACAG CCTCTGTGGT GTAATTGTAG AACCGATGGA AAATTGGTTG 
CAACTGATGC TGAACTGGGA TCCGCAACAG AGGGGTGGAG GTTTAGATCC TGAGACTAGT 
CGTCCAAAGT GTTTCCTAAT AATGGACCAC ATACTGAATT TGAAGATAGT ACACATCCTA 
AGCATGACCT CTGCCAAGAT TATTTCATTT CTCTTGCATC CTGAGGAAAG CCTTCATTCC 
CTTCAGATTC GGATTGAGTT TGAAACTGGA ATAAGTACTG GAAATCAGGA GCTGCTGTTG 
GAAACGGGGA TTTGCCTGGA CCCTCGGAAA CCTGCTTCAC AGTGTGTTAT TGATGGCGTG 
AGAGGCTGGG ATAGCTACAT GGTCTATTTG TTTGATAAAA GCAAAACTGT CTATGATGGA 
CCCTTTGATT CTCGGAGTCT TTCTGACTGT GTAAATTACA TTGTACAGGA TAGTAAAATC 
CAACTGCCAA TTCCTCAGCT GCGCAAGGTA TGGGCAGAAG CAGTTCACTA CGTGATTGGG 
CTAAAAGAAG ATTATAGCAG GCTTTTCCAG GGCCAGAGAG CTGCCATGCT CAGTCTTCTT 
CGGTATAATG CCAACCTAAT CAAAATGAAA AACAATATGG TGTCAGCATC TCAGCAGCTG 
AAAGCAAAGC TGGAATTCTT TCATCAAAGC ATTCGCCTCG ACTTGGAAAG ATACAGCGAC 
CAGATGGCTT ATGGCATATC TTCAGAAAAG ATGCTCAAAG CCTGGAAGGA GATGCAAGAG 
AAGGCCTCTC AGTGTGCACA GGCTGAAGAT ATAGGATATC TGGATGAACA GATCATGGCT 
TTGCACACAG AGATCGTAGA GCTTCAGAAG AGTCCATATG CAAGGCGCCA AGGAGAGGTT 
ATGGAGAGCT TGGAACAGAG AGCCATAGAC CTGTACAAGC AATTAAAAAC AAGACCTCCA 
GATCATGCCT ACAGTGACAG CACGGACATG GTGAAGATCA TTGTGCAGAC AGTACAAAGC 
CAAGACCGAG TTCTCAAGGA GCTTTTCGGC CATCTCAGCA AGCTCTTGGG CTGTAAGCAG 
AAGATCATTG ACTTGCTTCC AAAGATTGAA GTGGCTCTAA ATAACATCAA GGAAGCTGAC 
AACTCAGTGA TGCAGATGCA GGGCAAAAGA CAAAGGGAGA TATGGCATTT GTTGAAAATT 
GCTTGTACGC AGAGCTCTTC TCGATCACTG GTAAGCTCCA GCCTGGAAGG GACAGCCTCA 
ACTCCAGCAG TCACATGGCT CCAACAGAGC TCCTCAGGGA ACATACCTCA CCCTCTGTCA 
TCTCTGGCAA CTCCTGAAGA CAGGGAAAAT TTTGTCCATG TGATAGAAGA GAACCTGAAT 
TACCTTGAAC TCTTTAGCAG TATTTTGCAG GAGGTGAGAC AGGAGCAGAA CAACAGCATG 
ATGAGTTTTG ATTGGAGCTG GCTGAAATAG