spk-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	spk-1
Entrez Gene ID	176328
Full Name	SR Protein Kinase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025962.2	NP_001021133.1	Serine/threonine-protein kinase spk-1
NM_001025962.3	NP_001021133.1	Serine/threonine-protein kinase spk-1
NM_001025963.5	NP_001021134.1	Serine/threonine-protein kinase spk-1
NM_001306716.2	NP_001293645.1	Protein kinase domain-containing protein
NM_001025963.4	NP_001021134.1	Serine/threonine-protein kinase spk-1
NM_001306716.1	NP_001293645.1	Serine/threonine-protein kinase spk-1
NM_066679.5	NP_499080.3	Serine/threonine-protein kinase spk-1
NM_066679.4	NP_499080.3	Serine/threonine-protein kinase spk-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Srpk3	NP_062658.1
Bos taurus (cattle)	SRPK3	NP_001076859.1
Danio rerio (zebrafish)	srpk3	XP_694973.5
Xenopus tropicalis (tropical clawed frog)	srpk2	NP_001096257.1
Rattus norvegicus (Norway rat)	Srpk3	NP_908934.1
Homo sapiens (human)	SRPK3	NP_055185.2
Pan troglodytes (chimpanzee)	SRPK3	XP_003317820.2
	SRPK3	XP_002806501.1
Canis lupus familiaris (dog)	SRPK3	XP_855347.2
Caenorhabditis elegans (roundworm)	spk-1	NP_001021133.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study concludes that programmed cell death in C. elegans is regulated by an alternative splicing event controlled by the SR protein kinase SPK-1
Title: SPK-1, an SR protein kinase, inhibits programmed cell death in Caenorhabditis elegans.

The following spk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340091	NM_001025963.5 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa341776	NM_001025962.3 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa341862	NM_001306716.2 Latest version!	Caenorhabditis elegans Protein kinase domain-containing protein (spk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa340092	NM_066679.5 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa341776	NM_001025962.2	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa340091	NM_001025963.4	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa341862	NM_001306716.1	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa340092	NM_066679.4	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spk-1 ( NM_001025963.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021134.1 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

Price & Availability↑

CloneID	OCa340091
Clone ID Related Accession (Same CDS sequence)	NM_001025963.5 , NM_001025963.4
Accession Version	NM_001025963.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001025963.4.

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGC AAAATGGTGT CCGAATGCGT CCTCCATCTT TGCTCTTCAA TGGTCCGATT 
CCCCAACTGC TTCAAGGCAG TTCTTGTGTG AATACGCCAT CGTCTCCAAG AACGGTTCCA 
CCGCCACCAC CATCTCTCTA TCCACAGGTT GGATGCGTCT GCAATCAGAC GTATCATCTG 
ACACAGGAGT TGGAGAACTT CAACGCTTCA CAAGTGGAAG ATGTCACCAT GGAAGATACT 
GTCAATGAAA ATGGAAACAG AAACAAGGTG GAAATCAGGA GCCCTGATCG TTTCGATAGA 
ACCACGCTGA CTCCATTCAG TGATCCGGAG AGCAAGTTTG GAGATTTGGC CTCACCATCC 
GCCGAATATC TGAGCTCGCC CATGAGCCAG TTGCCTCCAG GTGGTATCCT GCCTGCTCCT 
CCAGTTGGCC CCAACATTGG TGATCCATAT TGTGACATCG ACGTCAAAAT CGCTGATCTC 
GGAAACGCGT GTTGGGTGAA TCATCACTAC ACGGACGATA TTCAAACTCG TCAGTATAGA 
GCACTTGAAG TGTTGATCGG TTCAGGATAC GGACCTCCAG CTGATATTTG GAGTACCGCG 
TGTATGGCTT TTGAATTGGC AACCGGTGAC TATTTGTTTG AACCACACCA AGGAGATAAC 
TATTCACGCG ATGAAGATCA TCTTGCGCAC ATTTCCGAAC TTCTTGGAGC AATTCCACCG 
TCAATTTACA AAAAGGGTAA ACATTGGAGA GAGTTCTTCC ACAAAAATGG ACACTTACTT 
CACATTCACC AACTTAAACC ATGGTCACTA TACGAGGTTT TGAGACAAAA ATACGAGTGG 
TCTCATGAAG ACGCGCAGCA GTTTGAAAGT TTCCTTCGTC CGATGCTTGA TTTCGATCAG 
GAGAAACGAT CTACGGCCAA AATTGCATTG AAACATCCGT TCTTGTTACC ATTTGGAGGA 
AGAGCGCCAA AATCTGATTG TCCACCTGAG CTTCTGAGCA AGATGTTTCC GGATGGATTG 
ATTCCCGAGC CGTTCGACGG AAATGAGCAT CAGGAAGTTT ACCGTGACGA AAATGATAGT 
AGATCGGCGA GCGAGAGGAG TGCCAATAGC AGGTCTGCGG GCGGCAGCGA CGACGACGAC 
GAGGAGGAGT TCAACATGAA TCGACCTGGT CCATCGGGGG TGATAACAAA CAATGAAACG 
ACCGACATTT CTGACATTGA ACGCTTTCAG CTGGATTTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021134.1
CDS	29..2353
Translation	MGGPEKNSTVPLPNKKRKKKTNKKKPTAPNTPTSPQAGEKNANLKNGTVTTNGSNHVERTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPQNGVRMRPPSLLFNGPIPQLLQGSSCVNTPSSPRTVPPPPPSLYPQVGCVCNQTYHLTQELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_001025963.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025963.5

ORF Insert Sequence:

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGC AAAATGGTGT CCGAATGCGT CCTCCATCTT TGCTCTTCAA TGGTCCGATT 
CCCCAACTGC TTCAAGGCAG TTCTTGTGTG AATACGCCAT CGTCTCCAAG AACGGTTCCA 
CCGCCACCAC CATCTCTCTA TCCACAGGTT GGATGCGTCT GCAATCAGAC GTATCATCTG 
ACACAGGAGT TGGAGAACTT CAACGCTTCA CAAGTGGAAG ATGTCACCAT GGAAGATACT 
GTCAATGAAA ATGGAAACAG AAACAAGGTG GAAATCAGGA GCCCTGATCG TTTCGATAGA 
ACCACGCTGA CTCCATTCAG TGATCCGGAG AGCAAGTTTG GAGATTTGGC CTCACCATCC 
GCCGAATATC TGAGCTCGCC CATGAGCCAG TTGCCTCCAG GTGGTATCCT GCCTGCTCCT 
CCAGTTGGCC CCAACATTGG TGATCCATAT TGTGACATCG ACGTCAAAAT CGCTGATCTC 
GGAAACGCGT GTTGGGTGAA TCATCACTAC ACGGACGATA TTCAAACTCG TCAGTATAGA 
GCACTTGAAG TGTTGATCGG TTCAGGATAC GGACCTCCAG CTGATATTTG GAGTACCGCG 
TGTATGGCTT TTGAATTGGC AACCGGTGAC TATTTGTTTG AACCACACCA AGGAGATAAC 
TATTCACGCG ATGAAGATCA TCTTGCGCAC ATTTCCGAAC TTCTTGGAGC AATTCCACCG 
TCAATTTACA AAAAGGGTAA ACATTGGAGA GAGTTCTTCC ACAAAAATGG ACACTTACTT 
CACATTCACC AACTTAAACC ATGGTCACTA TACGAGGTTT TGAGACAAAA ATACGAGTGG 
TCTCATGAAG ACGCGCAGCA GTTTGAAAGT TTCCTTCGTC CGATGCTTGA TTTCGATCAG 
GAGAAACGAT CTACGGCCAA AATTGCATTG AAACATCCGT TCTTGTTACC ATTTGGAGGA 
AGAGCGCCAA AATCTGATTG TCCACCTGAG CTTCTGAGCA AGATGTTTCC GGATGGATTG 
ATTCCCGAGC CGTTCGACGG AAATGAGCAT CAGGAAGTTT ACCGTGACGA AAATGATAGT 
AGATCGGCGA GCGAGAGGAG TGCCAATAGC AGGTCTGCGG GCGGCAGCGA CGACGACGAC 
GAGGAGGAGT TCAACATGAA TCGACCTGGT CCATCGGGGG TGATAACAAA CAATGAAACG 
ACCGACATTT CTGACATTGA ACGCTTTCAG CTGGATTTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_001025963.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021134.1 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

Price & Availability↑

CloneID	OCa340091
Clone ID Related Accession (Same CDS sequence)	NM_001025963.5 , NM_001025963.4
Accession Version	NM_001025963.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001025963.3. COMPLETENESS: incomplete on both ends.

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGC AAAATGGTGT CCGAATGCGT CCTCCATCTT TGCTCTTCAA TGGTCCGATT 
CCCCAACTGC TTCAAGGCAG TTCTTGTGTG AATACGCCAT CGTCTCCAAG AACGGTTCCA 
CCGCCACCAC CATCTCTCTA TCCACAGGTT GGATGCGTCT GCAATCAGAC GTATCATCTG 
ACACAGGAGT TGGAGAACTT CAACGCTTCA CAAGTGGAAG ATGTCACCAT GGAAGATACT 
GTCAATGAAA ATGGAAACAG AAACAAGGTG GAAATCAGGA GCCCTGATCG TTTCGATAGA 
ACCACGCTGA CTCCATTCAG TGATCCGGAG AGCAAGTTTG GAGATTTGGC CTCACCATCC 
GCCGAATATC TGAGCTCGCC CATGAGCCAG TTGCCTCCAG GTGGTATCCT GCCTGCTCCT 
CCAGTTGGCC CCAACATTGG TGATCCATAT TGTGACATCG ACGTCAAAAT CGCTGATCTC 
GGAAACGCGT GTTGGGTGAA TCATCACTAC ACGGACGATA TTCAAACTCG TCAGTATAGA 
GCACTTGAAG TGTTGATCGG TTCAGGATAC GGACCTCCAG CTGATATTTG GAGTACCGCG 
TGTATGGCTT TTGAATTGGC AACCGGTGAC TATTTGTTTG AACCACACCA AGGAGATAAC 
TATTCACGCG ATGAAGATCA TCTTGCGCAC ATTTCCGAAC TTCTTGGAGC AATTCCACCG 
TCAATTTACA AAAAGGGTAA ACATTGGAGA GAGTTCTTCC ACAAAAATGG ACACTTACTT 
CACATTCACC AACTTAAACC ATGGTCACTA TACGAGGTTT TGAGACAAAA ATACGAGTGG 
TCTCATGAAG ACGCGCAGCA GTTTGAAAGT TTCCTTCGTC CGATGCTTGA TTTCGATCAG 
GAGAAACGAT CTACGGCCAA AATTGCATTG AAACATCCGT TCTTGTTACC ATTTGGAGGA 
AGAGCGCCAA AATCTGATTG TCCACCTGAG CTTCTGAGCA AGATGTTTCC GGATGGATTG 
ATTCCCGAGC CGTTCGACGG AAATGAGCAT CAGGAAGTTT ACCGTGACGA AAATGATAGT 
AGATCGGCGA GCGAGAGGAG TGCCAATAGC AGGTCTGCGG GCGGCAGCGA CGACGACGAC 
GAGGAGGAGT TCAACATGAA TCGACCTGGT CCATCGGGGG TGATAACAAA CAATGAAACG 
ACCGACATTT CTGACATTGA ACGCTTTCAG CTGGATTTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021134.1
CDS	1..2325
Translation	MGGPEKNSTVPLPNKKRKKKTNKKKPTAPNTPTSPQAGEKNANLKNGTVTTNGSNHVERTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPQNGVRMRPPSLLFNGPIPQLLQGSSCVNTPSSPRTVPPPPPSLYPQVGCVCNQTYHLTQELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_001025963.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025963.4

ORF Insert Sequence:

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGC AAAATGGTGT CCGAATGCGT CCTCCATCTT TGCTCTTCAA TGGTCCGATT 
CCCCAACTGC TTCAAGGCAG TTCTTGTGTG AATACGCCAT CGTCTCCAAG AACGGTTCCA 
CCGCCACCAC CATCTCTCTA TCCACAGGTT GGATGCGTCT GCAATCAGAC GTATCATCTG 
ACACAGGAGT TGGAGAACTT CAACGCTTCA CAAGTGGAAG ATGTCACCAT GGAAGATACT 
GTCAATGAAA ATGGAAACAG AAACAAGGTG GAAATCAGGA GCCCTGATCG TTTCGATAGA 
ACCACGCTGA CTCCATTCAG TGATCCGGAG AGCAAGTTTG GAGATTTGGC CTCACCATCC 
GCCGAATATC TGAGCTCGCC CATGAGCCAG TTGCCTCCAG GTGGTATCCT GCCTGCTCCT 
CCAGTTGGCC CCAACATTGG TGATCCATAT TGTGACATCG ACGTCAAAAT CGCTGATCTC 
GGAAACGCGT GTTGGGTGAA TCATCACTAC ACGGACGATA TTCAAACTCG TCAGTATAGA 
GCACTTGAAG TGTTGATCGG TTCAGGATAC GGACCTCCAG CTGATATTTG GAGTACCGCG 
TGTATGGCTT TTGAATTGGC AACCGGTGAC TATTTGTTTG AACCACACCA AGGAGATAAC 
TATTCACGCG ATGAAGATCA TCTTGCGCAC ATTTCCGAAC TTCTTGGAGC AATTCCACCG 
TCAATTTACA AAAAGGGTAA ACATTGGAGA GAGTTCTTCC ACAAAAATGG ACACTTACTT 
CACATTCACC AACTTAAACC ATGGTCACTA TACGAGGTTT TGAGACAAAA ATACGAGTGG 
TCTCATGAAG ACGCGCAGCA GTTTGAAAGT TTCCTTCGTC CGATGCTTGA TTTCGATCAG 
GAGAAACGAT CTACGGCCAA AATTGCATTG AAACATCCGT TCTTGTTACC ATTTGGAGGA 
AGAGCGCCAA AATCTGATTG TCCACCTGAG CTTCTGAGCA AGATGTTTCC GGATGGATTG 
ATTCCCGAGC CGTTCGACGG AAATGAGCAT CAGGAAGTTT ACCGTGACGA AAATGATAGT 
AGATCGGCGA GCGAGAGGAG TGCCAATAGC AGGTCTGCGG GCGGCAGCGA CGACGACGAC 
GAGGAGGAGT TCAACATGAA TCGACCTGGT CCATCGGGGG TGATAACAAA CAATGAAACG 
ACCGACATTT CTGACATTGA ACGCTTTCAG CTGGATTTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_001025962.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021133.1 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

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CloneID	OCa341776
Clone ID Related Accession (Same CDS sequence)	NM_001025962.2 , NM_001025962.3
Accession Version	NM_001025962.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001025962.1. COMPLETENESS: incomplete on both ends.

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGG AGTTGGAGAA CTTCAACGCT TCACAAGTGG AAGATGTCAC CATGGAAGAT 
ACTGTCAATG AAAATGGAAA CAGAAACAAG GTGGAAATCA GGAGCCCTGA TCGTTTCGAT 
AGAACCACGC TGACTCCATT CAGTGATCCG GAGAGCAAGT TTGGAGATTT GGCCTCACCA 
TCCGCCGAAT ATCTGAGCTC GCCCATGAGC CAGTTGCCTC CAGGTGGTAT CCTGCCTGCT 
CCTCCAGTTG GCCCCAACAT TGGTGATCCA TATTGTGACA TCGACGTCAA AATCGCTGAT 
CTCGGAAACG CGTGTTGGGT GAATCATCAC TACACGGACG ATATTCAAAC TCGTCAGTAT 
AGAGCACTTG AAGTGTTGAT CGGTTCAGGA TACGGACCTC CAGCTGATAT TTGGAGTACC 
GCGTGTATGG CTTTTGAATT GGCAACCGGT GACTATTTGT TTGAACCACA CCAAGGAGAT 
AACTATTCAC GCGATGAAGA TCATCTTGCG CACATTTCCG AACTTCTTGG AGCAATTCCA 
CCGTCAATTT ACAAAAAGGG TAAACATTGG AGAGAGTTCT TCCACAAAAA TGGACACTTA 
CTTCACATTC ACCAACTTAA ACCATGGTCA CTATACGAGG TTTTGAGACA AAAATACGAG 
TGGTCTCATG AAGACGCGCA GCAGTTTGAA AGTTTCCTTC GTCCGATGCT TGATTTCGAT 
CAGGAGAAAC GATCTACGGC CAAAATTGCA TTGAAACATC CGTTCTTGTT ACCATTTGGA 
GGAAGAGCGC CAAAATCTGA TTGTCCACCT GAGCTTCTGA GCAAGATGTT TCCGGATGGA 
TTGATTCCCG AGCCGTTCGA CGGAAATGAG CATCAGGAAG TTTACCGTGA CGAAAATGAT 
AGTAGATCGG CGAGCGAGAG GAGTGCCAAT AGCAGGTCTG CGGGCGGCAG CGACGACGAC 
GACGAGGAGG AGTTCAACAT GAATCGACCT GGTCCATCGG GGCTGGATTT GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021133.1
CDS	1..2097
Translation	MGGPEKNSTVPLPNKKRKKKTNKKKPTAPNTPTSPQAGEKNANLKNGTVTTNGSNHVERTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGLDLQ

Target ORF information:

RefSeq Version	NM_001025962.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025962.2

ORF Insert Sequence:

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGG AGTTGGAGAA CTTCAACGCT TCACAAGTGG AAGATGTCAC CATGGAAGAT 
ACTGTCAATG AAAATGGAAA CAGAAACAAG GTGGAAATCA GGAGCCCTGA TCGTTTCGAT 
AGAACCACGC TGACTCCATT CAGTGATCCG GAGAGCAAGT TTGGAGATTT GGCCTCACCA 
TCCGCCGAAT ATCTGAGCTC GCCCATGAGC CAGTTGCCTC CAGGTGGTAT CCTGCCTGCT 
CCTCCAGTTG GCCCCAACAT TGGTGATCCA TATTGTGACA TCGACGTCAA AATCGCTGAT 
CTCGGAAACG CGTGTTGGGT GAATCATCAC TACACGGACG ATATTCAAAC TCGTCAGTAT 
AGAGCACTTG AAGTGTTGAT CGGTTCAGGA TACGGACCTC CAGCTGATAT TTGGAGTACC 
GCGTGTATGG CTTTTGAATT GGCAACCGGT GACTATTTGT TTGAACCACA CCAAGGAGAT 
AACTATTCAC GCGATGAAGA TCATCTTGCG CACATTTCCG AACTTCTTGG AGCAATTCCA 
CCGTCAATTT ACAAAAAGGG TAAACATTGG AGAGAGTTCT TCCACAAAAA TGGACACTTA 
CTTCACATTC ACCAACTTAA ACCATGGTCA CTATACGAGG TTTTGAGACA AAAATACGAG 
TGGTCTCATG AAGACGCGCA GCAGTTTGAA AGTTTCCTTC GTCCGATGCT TGATTTCGAT 
CAGGAGAAAC GATCTACGGC CAAAATTGCA TTGAAACATC CGTTCTTGTT ACCATTTGGA 
GGAAGAGCGC CAAAATCTGA TTGTCCACCT GAGCTTCTGA GCAAGATGTT TCCGGATGGA 
TTGATTCCCG AGCCGTTCGA CGGAAATGAG CATCAGGAAG TTTACCGTGA CGAAAATGAT 
AGTAGATCGG CGAGCGAGAG GAGTGCCAAT AGCAGGTCTG CGGGCGGCAG CGACGACGAC 
GACGAGGAGG AGTTCAACAT GAATCGACCT GGTCCATCGG GGCTGGATTT GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_001025962.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021133.1 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

Price & Availability↑

CloneID	OCa341776
Clone ID Related Accession (Same CDS sequence)	NM_001025962.2 , NM_001025962.3
Accession Version	NM_001025962.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001025962.2.

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGG AGTTGGAGAA CTTCAACGCT TCACAAGTGG AAGATGTCAC CATGGAAGAT 
ACTGTCAATG AAAATGGAAA CAGAAACAAG GTGGAAATCA GGAGCCCTGA TCGTTTCGAT 
AGAACCACGC TGACTCCATT CAGTGATCCG GAGAGCAAGT TTGGAGATTT GGCCTCACCA 
TCCGCCGAAT ATCTGAGCTC GCCCATGAGC CAGTTGCCTC CAGGTGGTAT CCTGCCTGCT 
CCTCCAGTTG GCCCCAACAT TGGTGATCCA TATTGTGACA TCGACGTCAA AATCGCTGAT 
CTCGGAAACG CGTGTTGGGT GAATCATCAC TACACGGACG ATATTCAAAC TCGTCAGTAT 
AGAGCACTTG AAGTGTTGAT CGGTTCAGGA TACGGACCTC CAGCTGATAT TTGGAGTACC 
GCGTGTATGG CTTTTGAATT GGCAACCGGT GACTATTTGT TTGAACCACA CCAAGGAGAT 
AACTATTCAC GCGATGAAGA TCATCTTGCG CACATTTCCG AACTTCTTGG AGCAATTCCA 
CCGTCAATTT ACAAAAAGGG TAAACATTGG AGAGAGTTCT TCCACAAAAA TGGACACTTA 
CTTCACATTC ACCAACTTAA ACCATGGTCA CTATACGAGG TTTTGAGACA AAAATACGAG 
TGGTCTCATG AAGACGCGCA GCAGTTTGAA AGTTTCCTTC GTCCGATGCT TGATTTCGAT 
CAGGAGAAAC GATCTACGGC CAAAATTGCA TTGAAACATC CGTTCTTGTT ACCATTTGGA 
GGAAGAGCGC CAAAATCTGA TTGTCCACCT GAGCTTCTGA GCAAGATGTT TCCGGATGGA 
TTGATTCCCG AGCCGTTCGA CGGAAATGAG CATCAGGAAG TTTACCGTGA CGAAAATGAT 
AGTAGATCGG CGAGCGAGAG GAGTGCCAAT AGCAGGTCTG CGGGCGGCAG CGACGACGAC 
GACGAGGAGG AGTTCAACAT GAATCGACCT GGTCCATCGG GGCTGGATTT GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021133.1
CDS	29..2125
Translation	MGGPEKNSTVPLPNKKRKKKTNKKKPTAPNTPTSPQAGEKNANLKNGTVTTNGSNHVERTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGLDLQ

Target ORF information:

RefSeq Version	NM_001025962.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025962.3

ORF Insert Sequence:

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 
ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 
AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 
GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 
TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 
GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 
TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 
GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 
GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 
TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 
TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 
GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 
GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 
TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 
GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 
GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 
AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 
GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 
AATGCACCGG AGTTGGAGAA CTTCAACGCT TCACAAGTGG AAGATGTCAC CATGGAAGAT 
ACTGTCAATG AAAATGGAAA CAGAAACAAG GTGGAAATCA GGAGCCCTGA TCGTTTCGAT 
AGAACCACGC TGACTCCATT CAGTGATCCG GAGAGCAAGT TTGGAGATTT GGCCTCACCA 
TCCGCCGAAT ATCTGAGCTC GCCCATGAGC CAGTTGCCTC CAGGTGGTAT CCTGCCTGCT 
CCTCCAGTTG GCCCCAACAT TGGTGATCCA TATTGTGACA TCGACGTCAA AATCGCTGAT 
CTCGGAAACG CGTGTTGGGT GAATCATCAC TACACGGACG ATATTCAAAC TCGTCAGTAT 
AGAGCACTTG AAGTGTTGAT CGGTTCAGGA TACGGACCTC CAGCTGATAT TTGGAGTACC 
GCGTGTATGG CTTTTGAATT GGCAACCGGT GACTATTTGT TTGAACCACA CCAAGGAGAT 
AACTATTCAC GCGATGAAGA TCATCTTGCG CACATTTCCG AACTTCTTGG AGCAATTCCA 
CCGTCAATTT ACAAAAAGGG TAAACATTGG AGAGAGTTCT TCCACAAAAA TGGACACTTA 
CTTCACATTC ACCAACTTAA ACCATGGTCA CTATACGAGG TTTTGAGACA AAAATACGAG 
TGGTCTCATG AAGACGCGCA GCAGTTTGAA AGTTTCCTTC GTCCGATGCT TGATTTCGAT 
CAGGAGAAAC GATCTACGGC CAAAATTGCA TTGAAACATC CGTTCTTGTT ACCATTTGGA 
GGAAGAGCGC CAAAATCTGA TTGTCCACCT GAGCTTCTGA GCAAGATGTT TCCGGATGGA 
TTGATTCCCG AGCCGTTCGA CGGAAATGAG CATCAGGAAG TTTACCGTGA CGAAAATGAT 
AGTAGATCGG CGAGCGAGAG GAGTGCCAAT AGCAGGTCTG CGGGCGGCAG CGACGACGAC 
GACGAGGAGG AGTTCAACAT GAATCGACCT GGTCCATCGG GGCTGGATTT GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_001306716.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293645.1 Caenorhabditis elegans Protein kinase domain-containing protein (spk-1), partial mRNA.

Price & Availability↑

CloneID	OCa341862
Clone ID Related Accession (Same CDS sequence)	NM_001306716.2 , NM_001306716.1
Accession Version	NM_001306716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein kinase domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001306716.1. COMPLETENESS: incomplete on the 5' end.

ATGGATCCTG GAGAACAACT TGGATCTGAC GATGAAGAAC AAGAAGATCC ACGTGATTAC 
AAACGAGGTG GATATCATCC AGTCAACATT GGTGATGTAT TCAATGCGAG ATATCATGTG 
ATTCGTAAAC TTGGATGGGG TCACTTTTCT ACGGTTTGGC TGGCGTGGGA CACTCAAGAC 
AAACGATTTG TTGCCATGAA AATAGTGAAG TCAGCGGAGC ATTACACGGA AGCGGCTCTT 
GATGAAATTA AGTTGTTGCT ATCCGTACGA AGTGCTGATC CAAATGACAT CGGATGCCAC 
AAGGTTGTTC AATTGCTCGA TGAATTCACT GTTACGGGAA TTAATGGTCA GCACGTTGCT 
ATGGTGTTTG AAGTTCTTGG GTGCAATCTG TTAAAGCTGA TTATACGCTC AAATTATCGA 
GGACTTCATC TGGAACAAGT TAGGAAAATA TGCAGACAAG TTCTGGAGGC GTTAGGATAT 
ATGCATGAAA AGTGCGGCAT CATTCATACT GATATTAAAC CTGAAAATGT GCTAATCACG 
ATGAGCCGAG AAGAGATCAA GATTATGGCT CAACATGCTG TAGTTGCCCG GAAGATGAAT 
ATGAAAATGA GTGGATCGGC TGTCAGTACT GCACCGGATC ATTTGGTGAA AATGGCTCAG 
GAGAATATGA CGAAAAACAA GAAGAAAAAG ATGAAGAAGA AGGCCAAAAA ACAACGAGAG 
AAGTTGGAAG CGGAATTGGC TGGACTCGAA GGCCTCAAAA TGGACGCTAA TGGGCTGCAG 
GAGGCATACA ATAATGCACC GGAGTTGGAG AACTTCAACG CTTCACAAGT GGAAGATGTC 
ACCATGGAAG ATACTGTCAA TGAAAATGGA AACAGAAACA AGGTGGAAAT CAGGAGCCCT 
GATCGTTTCG ATAGAACCAC GCTGACTCCA TTCAGTGATC CGGAGAGCAA GTTTGGAGAT 
TTGGCCTCAC CATCCGCCGA ATATCTGAGC TCGCCCATGA GCCAGTTGCC TCCAGGTGGT 
ATCCTGCCTG CTCCTCCAGT TGGCCCCAAC ATTGGTGATC CATATTGTGA CATCGACGTC 
AAAATCGCTG ATCTCGGAAA CGCGTGTTGG GTGAATCATC ACTACACGGA CGATATTCAA 
ACTCGTCAGT ATAGAGCACT TGAAGTGTTG ATCGGTTCAG GATACGGACC TCCAGCTGAT 
ATTTGGAGTA CCGCGTGTAT GGCTTTTGAA TTGGCAACCG GTGACTATTT GTTTGAACCA 
CACCAAGGAG ATAACTATTC ACGCGATGAA GATCATCTTG CGCACATTTC CGAACTTCTT 
GGAGCAATTC CACCGTCAAT TTACAAAAAG GGTAAACATT GGAGAGAGTT CTTCCACAAA 
AATGGACACT TACTTCACAT TCACCAACTT AAACCATGGT CACTATACGA GGTTTTGAGA 
CAAAAATACG AGTGGTCTCA TGAAGACGCG CAGCAGTTTG AAAGTTTCCT TCGTCCGATG 
CTTGATTTCG ATCAGGAGAA ACGATCTACG GCCAAAATTG CATTGAAACA TCCGTTCTTG 
TTACCATTTG GAGGAAGAGC GCCAAAATCT GATTGTCCAC CTGAGCTTCT GAGCAAGATG 
TTTCCGGATG GATTGATTCC CGAGCCGTTC GACGGAAATG AGCATCAGGA AGTTTACCGT 
GACGAAAATG ATAGTAGATC GGCGAGCGAG AGGAGTGCCA ATAGCAGGTC TGCGGGCGGC 
AGCGACGACG ACGACGAGGA GGAGTTCAAC ATGAATCGAC CTGGTCCATC GGGGGTGATA 
ACAAACAATG AAACGACCGA CATTTCTGAC ATTGAACGCT TTCAGCTGGA TTTGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293645.1
CDS	1..1860
Translation	MDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_001306716.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein kinase domain-containing protein (spk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306716.2

ORF Insert Sequence:

ATGGATCCTG GAGAACAACT TGGATCTGAC GATGAAGAAC AAGAAGATCC ACGTGATTAC 
AAACGAGGTG GATATCATCC AGTCAACATT GGTGATGTAT TCAATGCGAG ATATCATGTG 
ATTCGTAAAC TTGGATGGGG TCACTTTTCT ACGGTTTGGC TGGCGTGGGA CACTCAAGAC 
AAACGATTTG TTGCCATGAA AATAGTGAAG TCAGCGGAGC ATTACACGGA AGCGGCTCTT 
GATGAAATTA AGTTGTTGCT ATCCGTACGA AGTGCTGATC CAAATGACAT CGGATGCCAC 
AAGGTTGTTC AATTGCTCGA TGAATTCACT GTTACGGGAA TTAATGGTCA GCACGTTGCT 
ATGGTGTTTG AAGTTCTTGG GTGCAATCTG TTAAAGCTGA TTATACGCTC AAATTATCGA 
GGACTTCATC TGGAACAAGT TAGGAAAATA TGCAGACAAG TTCTGGAGGC GTTAGGATAT 
ATGCATGAAA AGTGCGGCAT CATTCATACT GATATTAAAC CTGAAAATGT GCTAATCACG 
ATGAGCCGAG AAGAGATCAA GATTATGGCT CAACATGCTG TAGTTGCCCG GAAGATGAAT 
ATGAAAATGA GTGGATCGGC TGTCAGTACT GCACCGGATC ATTTGGTGAA AATGGCTCAG 
GAGAATATGA CGAAAAACAA GAAGAAAAAG ATGAAGAAGA AGGCCAAAAA ACAACGAGAG 
AAGTTGGAAG CGGAATTGGC TGGACTCGAA GGCCTCAAAA TGGACGCTAA TGGGCTGCAG 
GAGGCATACA ATAATGCACC GGAGTTGGAG AACTTCAACG CTTCACAAGT GGAAGATGTC 
ACCATGGAAG ATACTGTCAA TGAAAATGGA AACAGAAACA AGGTGGAAAT CAGGAGCCCT 
GATCGTTTCG ATAGAACCAC GCTGACTCCA TTCAGTGATC CGGAGAGCAA GTTTGGAGAT 
TTGGCCTCAC CATCCGCCGA ATATCTGAGC TCGCCCATGA GCCAGTTGCC TCCAGGTGGT 
ATCCTGCCTG CTCCTCCAGT TGGCCCCAAC ATTGGTGATC CATATTGTGA CATCGACGTC 
AAAATCGCTG ATCTCGGAAA CGCGTGTTGG GTGAATCATC ACTACACGGA CGATATTCAA 
ACTCGTCAGT ATAGAGCACT TGAAGTGTTG ATCGGTTCAG GATACGGACC TCCAGCTGAT 
ATTTGGAGTA CCGCGTGTAT GGCTTTTGAA TTGGCAACCG GTGACTATTT GTTTGAACCA 
CACCAAGGAG ATAACTATTC ACGCGATGAA GATCATCTTG CGCACATTTC CGAACTTCTT 
GGAGCAATTC CACCGTCAAT TTACAAAAAG GGTAAACATT GGAGAGAGTT CTTCCACAAA 
AATGGACACT TACTTCACAT TCACCAACTT AAACCATGGT CACTATACGA GGTTTTGAGA 
CAAAAATACG AGTGGTCTCA TGAAGACGCG CAGCAGTTTG AAAGTTTCCT TCGTCCGATG 
CTTGATTTCG ATCAGGAGAA ACGATCTACG GCCAAAATTG CATTGAAACA TCCGTTCTTG 
TTACCATTTG GAGGAAGAGC GCCAAAATCT GATTGTCCAC CTGAGCTTCT GAGCAAGATG 
TTTCCGGATG GATTGATTCC CGAGCCGTTC GACGGAAATG AGCATCAGGA AGTTTACCGT 
GACGAAAATG ATAGTAGATC GGCGAGCGAG AGGAGTGCCA ATAGCAGGTC TGCGGGCGGC 
AGCGACGACG ACGACGAGGA GGAGTTCAAC ATGAATCGAC CTGGTCCATC GGGGGTGATA 
ACAAACAATG AAACGACCGA CATTTCTGAC ATTGAACGCT TTCAGCTGGA TTTGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_001306716.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293645.1 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

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CloneID	OCa341862
Clone ID Related Accession (Same CDS sequence)	NM_001306716.2 , NM_001306716.1
Accession Version	NM_001306716.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGATCCTG GAGAACAACT TGGATCTGAC GATGAAGAAC AAGAAGATCC ACGTGATTAC 
AAACGAGGTG GATATCATCC AGTCAACATT GGTGATGTAT TCAATGCGAG ATATCATGTG 
ATTCGTAAAC TTGGATGGGG TCACTTTTCT ACGGTTTGGC TGGCGTGGGA CACTCAAGAC 
AAACGATTTG TTGCCATGAA AATAGTGAAG TCAGCGGAGC ATTACACGGA AGCGGCTCTT 
GATGAAATTA AGTTGTTGCT ATCCGTACGA AGTGCTGATC CAAATGACAT CGGATGCCAC 
AAGGTTGTTC AATTGCTCGA TGAATTCACT GTTACGGGAA TTAATGGTCA GCACGTTGCT 
ATGGTGTTTG AAGTTCTTGG GTGCAATCTG TTAAAGCTGA TTATACGCTC AAATTATCGA 
GGACTTCATC TGGAACAAGT TAGGAAAATA TGCAGACAAG TTCTGGAGGC GTTAGGATAT 
ATGCATGAAA AGTGCGGCAT CATTCATACT GATATTAAAC CTGAAAATGT GCTAATCACG 
ATGAGCCGAG AAGAGATCAA GATTATGGCT CAACATGCTG TAGTTGCCCG GAAGATGAAT 
ATGAAAATGA GTGGATCGGC TGTCAGTACT GCACCGGATC ATTTGGTGAA AATGGCTCAG 
GAGAATATGA CGAAAAACAA GAAGAAAAAG ATGAAGAAGA AGGCCAAAAA ACAACGAGAG 
AAGTTGGAAG CGGAATTGGC TGGACTCGAA GGCCTCAAAA TGGACGCTAA TGGGCTGCAG 
GAGGCATACA ATAATGCACC GGAGTTGGAG AACTTCAACG CTTCACAAGT GGAAGATGTC 
ACCATGGAAG ATACTGTCAA TGAAAATGGA AACAGAAACA AGGTGGAAAT CAGGAGCCCT 
GATCGTTTCG ATAGAACCAC GCTGACTCCA TTCAGTGATC CGGAGAGCAA GTTTGGAGAT 
TTGGCCTCAC CATCCGCCGA ATATCTGAGC TCGCCCATGA GCCAGTTGCC TCCAGGTGGT 
ATCCTGCCTG CTCCTCCAGT TGGCCCCAAC ATTGGTGATC CATATTGTGA CATCGACGTC 
AAAATCGCTG ATCTCGGAAA CGCGTGTTGG GTGAATCATC ACTACACGGA CGATATTCAA 
ACTCGTCAGT ATAGAGCACT TGAAGTGTTG ATCGGTTCAG GATACGGACC TCCAGCTGAT 
ATTTGGAGTA CCGCGTGTAT GGCTTTTGAA TTGGCAACCG GTGACTATTT GTTTGAACCA 
CACCAAGGAG ATAACTATTC ACGCGATGAA GATCATCTTG CGCACATTTC CGAACTTCTT 
GGAGCAATTC CACCGTCAAT TTACAAAAAG GGTAAACATT GGAGAGAGTT CTTCCACAAA 
AATGGACACT TACTTCACAT TCACCAACTT AAACCATGGT CACTATACGA GGTTTTGAGA 
CAAAAATACG AGTGGTCTCA TGAAGACGCG CAGCAGTTTG AAAGTTTCCT TCGTCCGATG 
CTTGATTTCG ATCAGGAGAA ACGATCTACG GCCAAAATTG CATTGAAACA TCCGTTCTTG 
TTACCATTTG GAGGAAGAGC GCCAAAATCT GATTGTCCAC CTGAGCTTCT GAGCAAGATG 
TTTCCGGATG GATTGATTCC CGAGCCGTTC GACGGAAATG AGCATCAGGA AGTTTACCGT 
GACGAAAATG ATAGTAGATC GGCGAGCGAG AGGAGTGCCA ATAGCAGGTC TGCGGGCGGC 
AGCGACGACG ACGACGAGGA GGAGTTCAAC ATGAATCGAC CTGGTCCATC GGGGGTGATA 
ACAAACAATG AAACGACCGA CATTTCTGAC ATTGAACGCT TTCAGCTGGA TTTGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293645.1
CDS	1..1860
Translation	MDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_001306716.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306716.1

ORF Insert Sequence:

ATGGATCCTG GAGAACAACT TGGATCTGAC GATGAAGAAC AAGAAGATCC ACGTGATTAC 
AAACGAGGTG GATATCATCC AGTCAACATT GGTGATGTAT TCAATGCGAG ATATCATGTG 
ATTCGTAAAC TTGGATGGGG TCACTTTTCT ACGGTTTGGC TGGCGTGGGA CACTCAAGAC 
AAACGATTTG TTGCCATGAA AATAGTGAAG TCAGCGGAGC ATTACACGGA AGCGGCTCTT 
GATGAAATTA AGTTGTTGCT ATCCGTACGA AGTGCTGATC CAAATGACAT CGGATGCCAC 
AAGGTTGTTC AATTGCTCGA TGAATTCACT GTTACGGGAA TTAATGGTCA GCACGTTGCT 
ATGGTGTTTG AAGTTCTTGG GTGCAATCTG TTAAAGCTGA TTATACGCTC AAATTATCGA 
GGACTTCATC TGGAACAAGT TAGGAAAATA TGCAGACAAG TTCTGGAGGC GTTAGGATAT 
ATGCATGAAA AGTGCGGCAT CATTCATACT GATATTAAAC CTGAAAATGT GCTAATCACG 
ATGAGCCGAG AAGAGATCAA GATTATGGCT CAACATGCTG TAGTTGCCCG GAAGATGAAT 
ATGAAAATGA GTGGATCGGC TGTCAGTACT GCACCGGATC ATTTGGTGAA AATGGCTCAG 
GAGAATATGA CGAAAAACAA GAAGAAAAAG ATGAAGAAGA AGGCCAAAAA ACAACGAGAG 
AAGTTGGAAG CGGAATTGGC TGGACTCGAA GGCCTCAAAA TGGACGCTAA TGGGCTGCAG 
GAGGCATACA ATAATGCACC GGAGTTGGAG AACTTCAACG CTTCACAAGT GGAAGATGTC 
ACCATGGAAG ATACTGTCAA TGAAAATGGA AACAGAAACA AGGTGGAAAT CAGGAGCCCT 
GATCGTTTCG ATAGAACCAC GCTGACTCCA TTCAGTGATC CGGAGAGCAA GTTTGGAGAT 
TTGGCCTCAC CATCCGCCGA ATATCTGAGC TCGCCCATGA GCCAGTTGCC TCCAGGTGGT 
ATCCTGCCTG CTCCTCCAGT TGGCCCCAAC ATTGGTGATC CATATTGTGA CATCGACGTC 
AAAATCGCTG ATCTCGGAAA CGCGTGTTGG GTGAATCATC ACTACACGGA CGATATTCAA 
ACTCGTCAGT ATAGAGCACT TGAAGTGTTG ATCGGTTCAG GATACGGACC TCCAGCTGAT 
ATTTGGAGTA CCGCGTGTAT GGCTTTTGAA TTGGCAACCG GTGACTATTT GTTTGAACCA 
CACCAAGGAG ATAACTATTC ACGCGATGAA GATCATCTTG CGCACATTTC CGAACTTCTT 
GGAGCAATTC CACCGTCAAT TTACAAAAAG GGTAAACATT GGAGAGAGTT CTTCCACAAA 
AATGGACACT TACTTCACAT TCACCAACTT AAACCATGGT CACTATACGA GGTTTTGAGA 
CAAAAATACG AGTGGTCTCA TGAAGACGCG CAGCAGTTTG AAAGTTTCCT TCGTCCGATG 
CTTGATTTCG ATCAGGAGAA ACGATCTACG GCCAAAATTG CATTGAAACA TCCGTTCTTG 
TTACCATTTG GAGGAAGAGC GCCAAAATCT GATTGTCCAC CTGAGCTTCT GAGCAAGATG 
TTTCCGGATG GATTGATTCC CGAGCCGTTC GACGGAAATG AGCATCAGGA AGTTTACCGT 
GACGAAAATG ATAGTAGATC GGCGAGCGAG AGGAGTGCCA ATAGCAGGTC TGCGGGCGGC 
AGCGACGACG ACGACGAGGA GGAGTTCAAC ATGAATCGAC CTGGTCCATC GGGGGTGATA 
ACAAACAATG AAACGACCGA CATTTCTGAC ATTGAACGCT TTCAGCTGGA TTTGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_066679.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499080.3 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

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CloneID	OCa340092
Clone ID Related Accession (Same CDS sequence)	NM_066679.5 , NM_066679.4
Accession Version	NM_066679.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066679.4.

ATGTTTGTGG TTCTATTCTG TAGATGGCTC AATGGCTTCA GCCAGCATCG AGAACGACTT 
TATAAATTCG AAGATGAAGC CGTTCAGAGA CGCCGCCGTT TTCGTGTTCA TTCAGAGGAA 
TCGGAAGAAC TGAATGACCA CGAATCGTAT TCTGAAACTG ATATTTGTAC ACAGCTTTTG 
GCATCATCTG CAGACGTAAC CTGTCATATA AATGTTGATT TGGTCAACCA GAATCGATTT 
TATTTCTTTG TAAAGCAAAG AAATAAAAAA GAAAATTCGA CCAATGAAGA AGAAGAAATC 
TCTTCCCAAG AAATAAATAC GAGTACACAA AATGAAGAGA CAGAAGTTAA TTTAGTGTTC 
GCTAGTGCGG AAGATAATGG AGGCGTCGCG TCGAATTTCG ATATATCAGA CGCTCTAATG 
GTTACCTCTA ACATTTCCGA AGTCAGTAAA CTACCGGAAA CGTTGTGCGA AGAGGGTGAA 
ACTGTACAAC AAAAAAGTGT TGTAAATGAG AATGATCATT CGAATGAGGA CGATGAACAG 
TCGCTACAAT CACAAGATGG TTCAAGATGC TCTGATGAAG ACATGAACTC TTGCGTATCT 
GCCTCAGATG AAGAGGACGT TGAATCTCAA GATGATTCTT TTCATGTGAA TGATGCTACC 
GAAGAATCAA TTGATTCGGT AAGCTCTATT GAATCACAAG AAGCAGAAGA GTCAGCAACT 
GAAGATTTGG CGTCGTGTCA TTCGAACGAC GATAAAAATG AAAAAGATGT TCTTGTCGAT 
GAGGACACAT CCAAATACGA TAACCTTCCG GTGGAAATGC GGTCAGCAAA AGAGGGTGAA 
GAGAGTGAAG GGACGATCGA TTCTTCAGTC TCCTCGTCGA CATCGTCTTC CTCAACGGGC 
GACGACGGGG ACGACAGTGC TACCTCTTAC GACTCAGAAG ATATCGAAAT TCAGATGTTC 
GAGTATGATC TGGGAACCGC ATGCGCTTCA GCTTCTATTT CTATTCCTCG TCCATCTATA 
ATTCCAAGAA ATAACAAAAA GACCGAAGTC AATGCTAATG AAGAGCGTTT GGACGATTTA 
AGTGTGAGCC CCGGACGAAG TGATTCTCCT GGAGGCGGCG GTGGTGGACA TTCAGATTCA 
TTCCAAGATC CAATGGATCC TGGAGAACAA CTTGGATCTG ACGATGAAGA ACAAGAAGAT 
CCACGTGATT ACAAACGAGG TGGATATCAT CCAGTCAACA TTGGTGATGT ATTCAATGCG 
AGATATCATG TGATTCGTAA ACTTGGATGG GGTCACTTTT CTACGGTTTG GCTGGCGTGG 
GACACTCAAG ACAAACGATT TGTTGCCATG AAAATAGTGA AGTCAGCGGA GCATTACACG 
GAAGCGGCTC TTGATGAAAT TAAGTTGTTG CTATCCGTAC GAAGTGCTGA TCCAAATGAC 
ATCGGATGCC ACAAGGTTGT TCAATTGCTC GATGAATTCA CTGTTACGGG AATTAATGGT 
CAGCACGTTG CTATGGTGTT TGAAGTTCTT GGGTGCAATC TGTTAAAGCT GATTATACGC 
TCAAATTATC GAGGACTTCA TCTGGAACAA GTTAGGAAAA TATGCAGACA AGTTCTGGAG 
GCGTTAGGAT ATATGCATGA AAAGTGCGGC ATCATTCATA CTGATATTAA ACCTGAAAAT 
GTGCTAATCA CGATGAGCCG AGAAGAGATC AAGATTATGG CTCAACATGC TGTAGTTGCC 
CGGAAGATGA ATATGAAAAT GAGTGGATCG GCTGTCAGTA CTGCACCGGA TCATTTGGTG 
AAAATGGCTC AGGAGAATAT GACGAAAAAC AAGAAGAAAA AGATGAAGAA GAAGGCCAAA 
AAACAACGAG AGAAGTTGGA AGCGGAATTG GCTGGACTCG AAGGCCTCAA AATGGACGCT 
AATGGGCTGC AGGAGGCATA CAATAATGCA CCGGAGTTGG AGAACTTCAA CGCTTCACAA 
GTGGAAGATG TCACCATGGA AGATACTGTC AATGAAAATG GAAACAGAAA CAAGGTGGAA 
ATCAGGAGCC CTGATCGTTT CGATAGAACC ACGCTGACTC CATTCAGTGA TCCGGAGAGC 
AAGTTTGGAG ATTTGGCCTC ACCATCCGCC GAATATCTGA GCTCGCCCAT GAGCCAGTTG 
CCTCCAGGTG GTATCCTGCC TGCTCCTCCA GTTGGCCCCA ACATTGGTGA TCCATATTGT 
GACATCGACG TCAAAATCGC TGATCTCGGA AACGCGTGTT GGGTGAATCA TCACTACACG 
GACGATATTC AAACTCGTCA GTATAGAGCA CTTGAAGTGT TGATCGGTTC AGGATACGGA 
CCTCCAGCTG ATATTTGGAG TACCGCGTGT ATGGCTTTTG AATTGGCAAC CGGTGACTAT 
TTGTTTGAAC CACACCAAGG AGATAACTAT TCACGCGATG AAGATCATCT TGCGCACATT 
TCCGAACTTC TTGGAGCAAT TCCACCGTCA ATTTACAAAA AGGGTAAACA TTGGAGAGAG 
TTCTTCCACA AAAATGGACA CTTACTTCAC ATTCACCAAC TTAAACCATG GTCACTATAC 
GAGGTTTTGA GACAAAAATA CGAGTGGTCT CATGAAGACG CGCAGCAGTT TGAAAGTTTC 
CTTCGTCCGA TGCTTGATTT CGATCAGGAG AAACGATCTA CGGCCAAAAT TGCATTGAAA 
CATCCGTTCT TGTTACCATT TGGAGGAAGA GCGCCAAAAT CTGATTGTCC ACCTGAGCTT 
CTGAGCAAGA TGTTTCCGGA TGGATTGATT CCCGAGCCGT TCGACGGAAA TGAGCATCAG 
GAAGTTTACC GTGACGAAAA TGATAGTAGA TCGGCGAGCG AGAGGAGTGC CAATAGCAGG 
TCTGCGGGCG GCAGCGACGA CGACGACGAG GAGGAGTTCA ACATGAATCG ACCTGGTCCA 
TCGGGGGTGA TAACAAACAA TGAAACGACC GACATTTCTG ACATTGAACG CTTTCAGCTG 
GATTTGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499080.3
CDS	457..3468
Translation	MFVVLFCRWLNGFSQHRERLYKFEDEAVQRRRRFRVHSEESEELNDHESYSETDICTQLLASSADVTCHINVDLVNQNRFYFFVKQRNKKENSTNEEEEISSQEINTSTQNEETEVNLVFASAEDNGGVASNFDISDALMVTSNISEVSKLPETLCEEGETVQQKSVVNENDHSNEDDEQSLQSQDGSRCSDEDMNSCVSASDEEDVESQDDSFHVNDATEESIDSVSSIESQEAEESATEDLASCHSNDDKNEKDVLVDEDTSKYDNLPVEMRSAKEGEESEGTIDSSVSSSTSSSSTGDDGDDSATSYDSEDIEIQMFEYDLGTACASASISIPRPSIIPRNNKKTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_066679.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066679.5

ORF Insert Sequence:

ATGTTTGTGG TTCTATTCTG TAGATGGCTC AATGGCTTCA GCCAGCATCG AGAACGACTT 
TATAAATTCG AAGATGAAGC CGTTCAGAGA CGCCGCCGTT TTCGTGTTCA TTCAGAGGAA 
TCGGAAGAAC TGAATGACCA CGAATCGTAT TCTGAAACTG ATATTTGTAC ACAGCTTTTG 
GCATCATCTG CAGACGTAAC CTGTCATATA AATGTTGATT TGGTCAACCA GAATCGATTT 
TATTTCTTTG TAAAGCAAAG AAATAAAAAA GAAAATTCGA CCAATGAAGA AGAAGAAATC 
TCTTCCCAAG AAATAAATAC GAGTACACAA AATGAAGAGA CAGAAGTTAA TTTAGTGTTC 
GCTAGTGCGG AAGATAATGG AGGCGTCGCG TCGAATTTCG ATATATCAGA CGCTCTAATG 
GTTACCTCTA ACATTTCCGA AGTCAGTAAA CTACCGGAAA CGTTGTGCGA AGAGGGTGAA 
ACTGTACAAC AAAAAAGTGT TGTAAATGAG AATGATCATT CGAATGAGGA CGATGAACAG 
TCGCTACAAT CACAAGATGG TTCAAGATGC TCTGATGAAG ACATGAACTC TTGCGTATCT 
GCCTCAGATG AAGAGGACGT TGAATCTCAA GATGATTCTT TTCATGTGAA TGATGCTACC 
GAAGAATCAA TTGATTCGGT AAGCTCTATT GAATCACAAG AAGCAGAAGA GTCAGCAACT 
GAAGATTTGG CGTCGTGTCA TTCGAACGAC GATAAAAATG AAAAAGATGT TCTTGTCGAT 
GAGGACACAT CCAAATACGA TAACCTTCCG GTGGAAATGC GGTCAGCAAA AGAGGGTGAA 
GAGAGTGAAG GGACGATCGA TTCTTCAGTC TCCTCGTCGA CATCGTCTTC CTCAACGGGC 
GACGACGGGG ACGACAGTGC TACCTCTTAC GACTCAGAAG ATATCGAAAT TCAGATGTTC 
GAGTATGATC TGGGAACCGC ATGCGCTTCA GCTTCTATTT CTATTCCTCG TCCATCTATA 
ATTCCAAGAA ATAACAAAAA GACCGAAGTC AATGCTAATG AAGAGCGTTT GGACGATTTA 
AGTGTGAGCC CCGGACGAAG TGATTCTCCT GGAGGCGGCG GTGGTGGACA TTCAGATTCA 
TTCCAAGATC CAATGGATCC TGGAGAACAA CTTGGATCTG ACGATGAAGA ACAAGAAGAT 
CCACGTGATT ACAAACGAGG TGGATATCAT CCAGTCAACA TTGGTGATGT ATTCAATGCG 
AGATATCATG TGATTCGTAA ACTTGGATGG GGTCACTTTT CTACGGTTTG GCTGGCGTGG 
GACACTCAAG ACAAACGATT TGTTGCCATG AAAATAGTGA AGTCAGCGGA GCATTACACG 
GAAGCGGCTC TTGATGAAAT TAAGTTGTTG CTATCCGTAC GAAGTGCTGA TCCAAATGAC 
ATCGGATGCC ACAAGGTTGT TCAATTGCTC GATGAATTCA CTGTTACGGG AATTAATGGT 
CAGCACGTTG CTATGGTGTT TGAAGTTCTT GGGTGCAATC TGTTAAAGCT GATTATACGC 
TCAAATTATC GAGGACTTCA TCTGGAACAA GTTAGGAAAA TATGCAGACA AGTTCTGGAG 
GCGTTAGGAT ATATGCATGA AAAGTGCGGC ATCATTCATA CTGATATTAA ACCTGAAAAT 
GTGCTAATCA CGATGAGCCG AGAAGAGATC AAGATTATGG CTCAACATGC TGTAGTTGCC 
CGGAAGATGA ATATGAAAAT GAGTGGATCG GCTGTCAGTA CTGCACCGGA TCATTTGGTG 
AAAATGGCTC AGGAGAATAT GACGAAAAAC AAGAAGAAAA AGATGAAGAA GAAGGCCAAA 
AAACAACGAG AGAAGTTGGA AGCGGAATTG GCTGGACTCG AAGGCCTCAA AATGGACGCT 
AATGGGCTGC AGGAGGCATA CAATAATGCA CCGGAGTTGG AGAACTTCAA CGCTTCACAA 
GTGGAAGATG TCACCATGGA AGATACTGTC AATGAAAATG GAAACAGAAA CAAGGTGGAA 
ATCAGGAGCC CTGATCGTTT CGATAGAACC ACGCTGACTC CATTCAGTGA TCCGGAGAGC 
AAGTTTGGAG ATTTGGCCTC ACCATCCGCC GAATATCTGA GCTCGCCCAT GAGCCAGTTG 
CCTCCAGGTG GTATCCTGCC TGCTCCTCCA GTTGGCCCCA ACATTGGTGA TCCATATTGT 
GACATCGACG TCAAAATCGC TGATCTCGGA AACGCGTGTT GGGTGAATCA TCACTACACG 
GACGATATTC AAACTCGTCA GTATAGAGCA CTTGAAGTGT TGATCGGTTC AGGATACGGA 
CCTCCAGCTG ATATTTGGAG TACCGCGTGT ATGGCTTTTG AATTGGCAAC CGGTGACTAT 
TTGTTTGAAC CACACCAAGG AGATAACTAT TCACGCGATG AAGATCATCT TGCGCACATT 
TCCGAACTTC TTGGAGCAAT TCCACCGTCA ATTTACAAAA AGGGTAAACA TTGGAGAGAG 
TTCTTCCACA AAAATGGACA CTTACTTCAC ATTCACCAAC TTAAACCATG GTCACTATAC 
GAGGTTTTGA GACAAAAATA CGAGTGGTCT CATGAAGACG CGCAGCAGTT TGAAAGTTTC 
CTTCGTCCGA TGCTTGATTT CGATCAGGAG AAACGATCTA CGGCCAAAAT TGCATTGAAA 
CATCCGTTCT TGTTACCATT TGGAGGAAGA GCGCCAAAAT CTGATTGTCC ACCTGAGCTT 
CTGAGCAAGA TGTTTCCGGA TGGATTGATT CCCGAGCCGT TCGACGGAAA TGAGCATCAG 
GAAGTTTACC GTGACGAAAA TGATAGTAGA TCGGCGAGCG AGAGGAGTGC CAATAGCAGG 
TCTGCGGGCG GCAGCGACGA CGACGACGAG GAGGAGTTCA ACATGAATCG ACCTGGTCCA 
TCGGGGGTGA TAACAAACAA TGAAACGACC GACATTTCTG ACATTGAACG CTTTCAGCTG 
GATTTGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spk-1 ( NM_066679.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499080.3 Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

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CloneID	OCa340092
Clone ID Related Accession (Same CDS sequence)	NM_066679.5 , NM_066679.4
Accession Version	NM_066679.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase spk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066679.3. COMPLETENESS: incomplete on both ends.

ATGTTTGTGG TTCTATTCTG TAGATGGCTC AATGGCTTCA GCCAGCATCG AGAACGACTT 
TATAAATTCG AAGATGAAGC CGTTCAGAGA CGCCGCCGTT TTCGTGTTCA TTCAGAGGAA 
TCGGAAGAAC TGAATGACCA CGAATCGTAT TCTGAAACTG ATATTTGTAC ACAGCTTTTG 
GCATCATCTG CAGACGTAAC CTGTCATATA AATGTTGATT TGGTCAACCA GAATCGATTT 
TATTTCTTTG TAAAGCAAAG AAATAAAAAA GAAAATTCGA CCAATGAAGA AGAAGAAATC 
TCTTCCCAAG AAATAAATAC GAGTACACAA AATGAAGAGA CAGAAGTTAA TTTAGTGTTC 
GCTAGTGCGG AAGATAATGG AGGCGTCGCG TCGAATTTCG ATATATCAGA CGCTCTAATG 
GTTACCTCTA ACATTTCCGA AGTCAGTAAA CTACCGGAAA CGTTGTGCGA AGAGGGTGAA 
ACTGTACAAC AAAAAAGTGT TGTAAATGAG AATGATCATT CGAATGAGGA CGATGAACAG 
TCGCTACAAT CACAAGATGG TTCAAGATGC TCTGATGAAG ACATGAACTC TTGCGTATCT 
GCCTCAGATG AAGAGGACGT TGAATCTCAA GATGATTCTT TTCATGTGAA TGATGCTACC 
GAAGAATCAA TTGATTCGGT AAGCTCTATT GAATCACAAG AAGCAGAAGA GTCAGCAACT 
GAAGATTTGG CGTCGTGTCA TTCGAACGAC GATAAAAATG AAAAAGATGT TCTTGTCGAT 
GAGGACACAT CCAAATACGA TAACCTTCCG GTGGAAATGC GGTCAGCAAA AGAGGGTGAA 
GAGAGTGAAG GGACGATCGA TTCTTCAGTC TCCTCGTCGA CATCGTCTTC CTCAACGGGC 
GACGACGGGG ACGACAGTGC TACCTCTTAC GACTCAGAAG ATATCGAAAT TCAGATGTTC 
GAGTATGATC TGGGAACCGC ATGCGCTTCA GCTTCTATTT CTATTCCTCG TCCATCTATA 
ATTCCAAGAA ATAACAAAAA GACCGAAGTC AATGCTAATG AAGAGCGTTT GGACGATTTA 
AGTGTGAGCC CCGGACGAAG TGATTCTCCT GGAGGCGGCG GTGGTGGACA TTCAGATTCA 
TTCCAAGATC CAATGGATCC TGGAGAACAA CTTGGATCTG ACGATGAAGA ACAAGAAGAT 
CCACGTGATT ACAAACGAGG TGGATATCAT CCAGTCAACA TTGGTGATGT ATTCAATGCG 
AGATATCATG TGATTCGTAA ACTTGGATGG GGTCACTTTT CTACGGTTTG GCTGGCGTGG 
GACACTCAAG ACAAACGATT TGTTGCCATG AAAATAGTGA AGTCAGCGGA GCATTACACG 
GAAGCGGCTC TTGATGAAAT TAAGTTGTTG CTATCCGTAC GAAGTGCTGA TCCAAATGAC 
ATCGGATGCC ACAAGGTTGT TCAATTGCTC GATGAATTCA CTGTTACGGG AATTAATGGT 
CAGCACGTTG CTATGGTGTT TGAAGTTCTT GGGTGCAATC TGTTAAAGCT GATTATACGC 
TCAAATTATC GAGGACTTCA TCTGGAACAA GTTAGGAAAA TATGCAGACA AGTTCTGGAG 
GCGTTAGGAT ATATGCATGA AAAGTGCGGC ATCATTCATA CTGATATTAA ACCTGAAAAT 
GTGCTAATCA CGATGAGCCG AGAAGAGATC AAGATTATGG CTCAACATGC TGTAGTTGCC 
CGGAAGATGA ATATGAAAAT GAGTGGATCG GCTGTCAGTA CTGCACCGGA TCATTTGGTG 
AAAATGGCTC AGGAGAATAT GACGAAAAAC AAGAAGAAAA AGATGAAGAA GAAGGCCAAA 
AAACAACGAG AGAAGTTGGA AGCGGAATTG GCTGGACTCG AAGGCCTCAA AATGGACGCT 
AATGGGCTGC AGGAGGCATA CAATAATGCA CCGGAGTTGG AGAACTTCAA CGCTTCACAA 
GTGGAAGATG TCACCATGGA AGATACTGTC AATGAAAATG GAAACAGAAA CAAGGTGGAA 
ATCAGGAGCC CTGATCGTTT CGATAGAACC ACGCTGACTC CATTCAGTGA TCCGGAGAGC 
AAGTTTGGAG ATTTGGCCTC ACCATCCGCC GAATATCTGA GCTCGCCCAT GAGCCAGTTG 
CCTCCAGGTG GTATCCTGCC TGCTCCTCCA GTTGGCCCCA ACATTGGTGA TCCATATTGT 
GACATCGACG TCAAAATCGC TGATCTCGGA AACGCGTGTT GGGTGAATCA TCACTACACG 
GACGATATTC AAACTCGTCA GTATAGAGCA CTTGAAGTGT TGATCGGTTC AGGATACGGA 
CCTCCAGCTG ATATTTGGAG TACCGCGTGT ATGGCTTTTG AATTGGCAAC CGGTGACTAT 
TTGTTTGAAC CACACCAAGG AGATAACTAT TCACGCGATG AAGATCATCT TGCGCACATT 
TCCGAACTTC TTGGAGCAAT TCCACCGTCA ATTTACAAAA AGGGTAAACA TTGGAGAGAG 
TTCTTCCACA AAAATGGACA CTTACTTCAC ATTCACCAAC TTAAACCATG GTCACTATAC 
GAGGTTTTGA GACAAAAATA CGAGTGGTCT CATGAAGACG CGCAGCAGTT TGAAAGTTTC 
CTTCGTCCGA TGCTTGATTT CGATCAGGAG AAACGATCTA CGGCCAAAAT TGCATTGAAA 
CATCCGTTCT TGTTACCATT TGGAGGAAGA GCGCCAAAAT CTGATTGTCC ACCTGAGCTT 
CTGAGCAAGA TGTTTCCGGA TGGATTGATT CCCGAGCCGT TCGACGGAAA TGAGCATCAG 
GAAGTTTACC GTGACGAAAA TGATAGTAGA TCGGCGAGCG AGAGGAGTGC CAATAGCAGG 
TCTGCGGGCG GCAGCGACGA CGACGACGAG GAGGAGTTCA ACATGAATCG ACCTGGTCCA 
TCGGGGGTGA TAACAAACAA TGAAACGACC GACATTTCTG ACATTGAACG CTTTCAGCTG 
GATTTGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499080.3
CDS	1..3012
Translation	MFVVLFCRWLNGFSQHRERLYKFEDEAVQRRRRFRVHSEESEELNDHESYSETDICTQLLASSADVTCHINVDLVNQNRFYFFVKQRNKKENSTNEEEEISSQEINTSTQNEETEVNLVFASAEDNGGVASNFDISDALMVTSNISEVSKLPETLCEEGETVQQKSVVNENDHSNEDDEQSLQSQDGSRCSDEDMNSCVSASDEEDVESQDDSFHVNDATEESIDSVSSIESQEAEESATEDLASCHSNDDKNEKDVLVDEDTSKYDNLPVEMRSAKEGEESEGTIDSSVSSSTSSSSTGDDGDDSATSYDSEDIEIQMFEYDLGTACASASISIPRPSIIPRNNKKTEVNANEERLDDLSVSPGRSDSPGGGGGGHSDSFQDPMDPGEQLGSDDEEQEDPRDYKRGGYHPVNIGDVFNARYHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDIGCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEALGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMKMSGSAVSTAPDHLVKMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMDANGLQEAYNNAPELENFNASQVEDVTMEDTVNENGNRNKVEIRSPDRFDRTTLTPFSDPESKFGDLASPSAEYLSSPMSQLPPGGILPAPPVGPNIGDPYCDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVLIGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKKGKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRSTAKIALKHPFLLPFGGRAPKSDCPPELLSKMFPDGLIPEPFDGNEHQEVYRDENDSRSASERSANSRSAGGSDDDDEEEFNMNRPGPSGVITNNETTDISDIERFQLDLQ

Target ORF information:

RefSeq Version	NM_066679.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase spk-1 (spk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066679.4

ORF Insert Sequence:

ATGTTTGTGG TTCTATTCTG TAGATGGCTC AATGGCTTCA GCCAGCATCG AGAACGACTT 
TATAAATTCG AAGATGAAGC CGTTCAGAGA CGCCGCCGTT TTCGTGTTCA TTCAGAGGAA 
TCGGAAGAAC TGAATGACCA CGAATCGTAT TCTGAAACTG ATATTTGTAC ACAGCTTTTG 
GCATCATCTG CAGACGTAAC CTGTCATATA AATGTTGATT TGGTCAACCA GAATCGATTT 
TATTTCTTTG TAAAGCAAAG AAATAAAAAA GAAAATTCGA CCAATGAAGA AGAAGAAATC 
TCTTCCCAAG AAATAAATAC GAGTACACAA AATGAAGAGA CAGAAGTTAA TTTAGTGTTC 
GCTAGTGCGG AAGATAATGG AGGCGTCGCG TCGAATTTCG ATATATCAGA CGCTCTAATG 
GTTACCTCTA ACATTTCCGA AGTCAGTAAA CTACCGGAAA CGTTGTGCGA AGAGGGTGAA 
ACTGTACAAC AAAAAAGTGT TGTAAATGAG AATGATCATT CGAATGAGGA CGATGAACAG 
TCGCTACAAT CACAAGATGG TTCAAGATGC TCTGATGAAG ACATGAACTC TTGCGTATCT 
GCCTCAGATG AAGAGGACGT TGAATCTCAA GATGATTCTT TTCATGTGAA TGATGCTACC 
GAAGAATCAA TTGATTCGGT AAGCTCTATT GAATCACAAG AAGCAGAAGA GTCAGCAACT 
GAAGATTTGG CGTCGTGTCA TTCGAACGAC GATAAAAATG AAAAAGATGT TCTTGTCGAT 
GAGGACACAT CCAAATACGA TAACCTTCCG GTGGAAATGC GGTCAGCAAA AGAGGGTGAA 
GAGAGTGAAG GGACGATCGA TTCTTCAGTC TCCTCGTCGA CATCGTCTTC CTCAACGGGC 
GACGACGGGG ACGACAGTGC TACCTCTTAC GACTCAGAAG ATATCGAAAT TCAGATGTTC 
GAGTATGATC TGGGAACCGC ATGCGCTTCA GCTTCTATTT CTATTCCTCG TCCATCTATA 
ATTCCAAGAA ATAACAAAAA GACCGAAGTC AATGCTAATG AAGAGCGTTT GGACGATTTA 
AGTGTGAGCC CCGGACGAAG TGATTCTCCT GGAGGCGGCG GTGGTGGACA TTCAGATTCA 
TTCCAAGATC CAATGGATCC TGGAGAACAA CTTGGATCTG ACGATGAAGA ACAAGAAGAT 
CCACGTGATT ACAAACGAGG TGGATATCAT CCAGTCAACA TTGGTGATGT ATTCAATGCG 
AGATATCATG TGATTCGTAA ACTTGGATGG GGTCACTTTT CTACGGTTTG GCTGGCGTGG 
GACACTCAAG ACAAACGATT TGTTGCCATG AAAATAGTGA AGTCAGCGGA GCATTACACG 
GAAGCGGCTC TTGATGAAAT TAAGTTGTTG CTATCCGTAC GAAGTGCTGA TCCAAATGAC 
ATCGGATGCC ACAAGGTTGT TCAATTGCTC GATGAATTCA CTGTTACGGG AATTAATGGT 
CAGCACGTTG CTATGGTGTT TGAAGTTCTT GGGTGCAATC TGTTAAAGCT GATTATACGC 
TCAAATTATC GAGGACTTCA TCTGGAACAA GTTAGGAAAA TATGCAGACA AGTTCTGGAG 
GCGTTAGGAT ATATGCATGA AAAGTGCGGC ATCATTCATA CTGATATTAA ACCTGAAAAT 
GTGCTAATCA CGATGAGCCG AGAAGAGATC AAGATTATGG CTCAACATGC TGTAGTTGCC 
CGGAAGATGA ATATGAAAAT GAGTGGATCG GCTGTCAGTA CTGCACCGGA TCATTTGGTG 
AAAATGGCTC AGGAGAATAT GACGAAAAAC AAGAAGAAAA AGATGAAGAA GAAGGCCAAA 
AAACAACGAG AGAAGTTGGA AGCGGAATTG GCTGGACTCG AAGGCCTCAA AATGGACGCT 
AATGGGCTGC AGGAGGCATA CAATAATGCA CCGGAGTTGG AGAACTTCAA CGCTTCACAA 
GTGGAAGATG TCACCATGGA AGATACTGTC AATGAAAATG GAAACAGAAA CAAGGTGGAA 
ATCAGGAGCC CTGATCGTTT CGATAGAACC ACGCTGACTC CATTCAGTGA TCCGGAGAGC 
AAGTTTGGAG ATTTGGCCTC ACCATCCGCC GAATATCTGA GCTCGCCCAT GAGCCAGTTG 
CCTCCAGGTG GTATCCTGCC TGCTCCTCCA GTTGGCCCCA ACATTGGTGA TCCATATTGT 
GACATCGACG TCAAAATCGC TGATCTCGGA AACGCGTGTT GGGTGAATCA TCACTACACG 
GACGATATTC AAACTCGTCA GTATAGAGCA CTTGAAGTGT TGATCGGTTC AGGATACGGA 
CCTCCAGCTG ATATTTGGAG TACCGCGTGT ATGGCTTTTG AATTGGCAAC CGGTGACTAT 
TTGTTTGAAC CACACCAAGG AGATAACTAT TCACGCGATG AAGATCATCT TGCGCACATT 
TCCGAACTTC TTGGAGCAAT TCCACCGTCA ATTTACAAAA AGGGTAAACA TTGGAGAGAG 
TTCTTCCACA AAAATGGACA CTTACTTCAC ATTCACCAAC TTAAACCATG GTCACTATAC 
GAGGTTTTGA GACAAAAATA CGAGTGGTCT CATGAAGACG CGCAGCAGTT TGAAAGTTTC 
CTTCGTCCGA TGCTTGATTT CGATCAGGAG AAACGATCTA CGGCCAAAAT TGCATTGAAA 
CATCCGTTCT TGTTACCATT TGGAGGAAGA GCGCCAAAAT CTGATTGTCC ACCTGAGCTT 
CTGAGCAAGA TGTTTCCGGA TGGATTGATT CCCGAGCCGT TCGACGGAAA TGAGCATCAG 
GAAGTTTACC GTGACGAAAA TGATAGTAGA TCGGCGAGCG AGAGGAGTGC CAATAGCAGG 
TCTGCGGGCG GCAGCGACGA CGACGACGAG GAGGAGTTCA ACATGAATCG ACCTGGTCCA 
TCGGGGGTGA TAACAAACAA TGAAACGACC GACATTTCTG ACATTGAACG CTTTCAGCTG 
GATTTGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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spk-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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