IL6 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL6
Entrez Gene ID	102415101
Full Name	interleukin 6
Synonyms	IL-6
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290980.1	NP_001277909.1	interleukin-6 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

IGF-1 attenuates LPS induced pro-inflammatory cytokines expression in buffalo (Bubalus bubalis) granulosa cells.
Onnureddy K, Ravinder, Onteru SK, Singh D
Molecular immunology64(1)136-43(2015 Mar)

Molecular cloning, sequencing and phylogenetic analysis of inflammatory cytokines of swamp type buffalo contrasting with other bubaline breeds.
Mingala CN, Odbileg R, Konnai S, Ohashi K, Onuma M
Comparative immunology, microbiology and infectious diseases30(2)119-31(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present work studied the effect of IGF-1 on lipopolysaccharide mediated pro-inflammatory cytokine expression and aromatase genes' expressions as well as proliferation of buffalo granulosa cells.
Title: IGF-1 attenuates LPS induced pro-inflammatory cytokines expression in buffalo (Bubalus bubalis) granulosa cells.

The following IL6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu00080	NM_001290980.1 Latest version!	Bubalus bubalis interleukin 6 (IL6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL6 ( NM_001290980.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> NP_001277909.1 Bubalus bubalis interleukin 6 (IL6), mRNA.

Price & Availability↑

CloneID	OBu00080
Clone ID Related Accession (Same CDS sequence)	NM_001290980.1
Accession Version	NM_001290980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bubalus bubalis(water buffalo)
Product	interleukin-6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB246788.1. On Mar 20, 2014 this sequence version replaced XM_006074852.1. ##Evidence-Data-START## Transcript exon combination :: AB246788.1, AY347710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103886389, SAMEA103886404 [ECO:0000348] ##Evidence-Data-END##

ATGAACTCCC GCTTCACAAG CGCCTTCACT CCATTCGCTG TCTCCCTGGG GCTGCTCCTG 
GTGATGACTT CTGCTTTCCC TACCCCGGGT CCCCTGGGAG AAGATTTCAA AAATGACACC 
ACCCCAGGCA GACTACTTCT GACCACTCCA GAGAAAACCG AAGCTCTCAT TAAGCGCATG 
GTCGACAAAA TTTCTGCAAT GAGAAAGGAG ATATGTGAGA AGAATGACGA GTGTGAAAGC 
AGCAAGGACA CACTGGCAGA AAATAAGCTG AATCTTCCAA AAATGGAGGA AAAGGACGGA 
TGCTTCCAAT CTGGGTTCAA TCAGGCGATT TGCTTGATCA GAACCACTGC TGGTCTTCTG 
GAGTATCAGA TATACCTGGA CTACCTCCAG AATGAGTATG AGGGAAATCG GGAAAATGTA 
AGGGATTTGA GGAAAAATAT CAGAACACTG ATCCAGATCC TGAAGCAAAA GATCGCAGAT 
CTAATAACCA CTCCAGCCAC AAACACTGAC CTGCTGGAGA AGATGCAGTC CTCAAACGAG 
TGGGTAAAGA ACGCAAAGAT TATCCTCATC CTGAGAAACC TTGAGAATTT CCTACAGTTC 
AGCCTGAGAG CTATTCGGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277909.1
CDS	40..666
Translation	MNSRFTSAFTPFAVSLGLLLVMTSAFPTPGPLGEDFKNDTTPGRLLLTTPEKTEALIKRMVDKISAMRKEICEKNDECESSKDTLAENKLNLPKMEEKDGCFQSGFNQAICLIRTTAGLLEYQIYLDYLQNEYEGNRENVRDLRKNIRTLIQILKQKIADLITTPATNTDLLEKMQSSNEWVKNAKIILILRNLENFLQFSLRAIRMK

Target ORF information:

RefSeq Version	NM_001290980.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis interleukin 6 (IL6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290980.1

ORF Insert Sequence:

ATGAACTCCC GCTTCACAAG CGCCTTCACT CCATTCGCTG TCTCCCTGGG GCTGCTCCTG 
GTGATGACTT CTGCTTTCCC TACCCCGGGT CCCCTGGGAG AAGATTTCAA AAATGACACC 
ACCCCAGGCA GACTACTTCT GACCACTCCA GAGAAAACCG AAGCTCTCAT TAAGCGCATG 
GTCGACAAAA TTTCTGCAAT GAGAAAGGAG ATATGTGAGA AGAATGACGA GTGTGAAAGC 
AGCAAGGACA CACTGGCAGA AAATAAGCTG AATCTTCCAA AAATGGAGGA AAAGGACGGA 
TGCTTCCAAT CTGGGTTCAA TCAGGCGATT TGCTTGATCA GAACCACTGC TGGTCTTCTG 
GAGTATCAGA TATACCTGGA CTACCTCCAG AATGAGTATG AGGGAAATCG GGAAAATGTA 
AGGGATTTGA GGAAAAATAT CAGAACACTG ATCCAGATCC TGAAGCAAAA GATCGCAGAT 
CTAATAACCA CTCCAGCCAC AAACACTGAC CTGCTGGAGA AGATGCAGTC CTCAAACGAG 
TGGGTAAAGA ACGCAAAGAT TATCCTCATC CTGAGAAACC TTGAGAATTT CCTACAGTTC 
AGCCTGAGAG CTATTCGGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IGF-1 attenuates LPS induced pro-inflammatory cytokines expression in buffalo (Bubalus bubalis) granulosa cells.
Molecular immunology64(1)136-43(2015 Mar)
Onnureddy K,Ravinder,Onteru SK,Singh D

Molecular cloning, sequencing and phylogenetic analysis of inflammatory cytokines of swamp type buffalo contrasting with other bubaline breeds.
Comparative immunology, microbiology and infectious diseases30(2)119-31(2007 Mar)
Mingala CN,Odbileg R,Konnai S,Ohashi K,Onuma M