LOC103869811 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103869811
Entrez Gene ID	103869811
Full Name	probable cyclic nucleotide-gated ion channel 20, chloroplastic
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018659147.1	XP_018514663.1	probable cyclic nucleotide-gated ion channel 20, chloroplastic

Pathways from BioSystems
Gene Ontology

The following LOC103869811 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103869811 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr149216	XM_018659147.1 Latest version!	Brassica rapa probable cyclic nucleotide-gated ion channel 20, chloroplastic (LOC103869811), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103869811 ( XM_018659147.1 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_018514663.1 Brassica rapa probable cyclic nucleotide-gated ion channel 20, chloroplastic (LOC103869811), mRNA.

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CloneID	OBr149216
Clone ID Related Accession (Same CDS sequence)	XM_018659147.1
Accession Version	XM_018659147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	probable cyclic nucleotide-gated ion channel 20, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024799.1) and transcript sequence (FX700867.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 247 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGAAGG ATGATGTTCC CATGCTTCCA GTTTCAGACT CATCATCGTT ATCTCGTACT 
AGGCCTTTCA CTTCCAGGTC ACGTAGCGTT TCCCTCGCAA ACACTTCTTC GATCCTTGAT 
GGGTTCGACA GCTCGACTGT TGTTTTAGGC TACACAGGTC CTCTTCGAAC TCATAGAAGG 
CCTCCTTTAG TTCAGATGAG TGGTCCTCTC TCATCTACTC GCAGTTCTGA GCCTCTCTTT 
CTTCCTCCTC CTCCTACTTC CACTCGTGAT GTCTCCTCCT CTCAGCCTGA GAGGTACCCT 
TCCTTTGCTG CTCTCGAACA CAAAAACTCA GAGGAGGAGT TCGTATTGAA ACACGCACAT 
CTCTTGAGAT CTGGACAGTT GGGTATGTGT AATGATCCTT ACTGTACCAC TTGTCCTTCT 
TACTACAACC GGAAAGCTTC CCAAATCCCT ACTTCTAGAG TTTCTGCCAT TTTTGATTCC 
ACTTTCCATG ATGCTAAAGG TTGGGCTAGG AGGTTTGCTA CATCTATTAA TAGACACTTA 
CCAGGAATCA TGAATCCTCA CTCCAAGTTC GTTCAGAGAT GGACTAAGTT CTTTGCCTTT 
TCTTGCTTGT TAGCTATTTT TATAGATCCC CTCTTCTTCT TCCTCATATT AGTCAAGCAG 
AACAATAATT GCATTGAGAT TGATTGGCCG ATGGCTCACA CATTTGTAGC TGTTAGAAGT 
GTAACAGATG TTTTGTTCTC TGTAAACATC CTGCTTCAGT TCCGATTAGC CTATGTAGCT 
CCTGAGTCTA CGGTTGTTGG TGCTGGCCAG TTGGTAGACC ATCCAAGAAA AGTTGCTAGT 
CATTACTTCC GAGGAAAGTT TTTACTAGAT TTGTTCATAG TGATGCCACT TCCACAGATA 
TTGATATTAT GGATAATACC AGCAAACTTA GCCATATCTG GGGCAAACTA TGCAAAGAAC 
CTTTTACGTG CTGCAGTTCT TTTCCAATAC ATTCCCAAGT TATATAGGCT ACTACCACTT 
CTTGCTGGAC AAACACCGAC TGGCTTCATC TTTGAGTCGG CTTGGGCTAA TTTTTTTATT 
AATCTTCTTA CCTTCATGCT TGCTGGTCAT GTCGTTGGCT CTTGTTGGTA TCTTTTTGGT 
CTGCAGAGAG TTAATCAATG CCTTCGAGAT GCTTGCGGTA GTTCTGATCG TCCATGCAGA 
GAGCTTATAG ATTGTCGTGG TGAAACTAGC GCTTTATCAG CTGCGTGGAA AGATAATGCG 
AGTGCCCGTG CTTGTTTTCA AGAGGATGGT TTTCCTTATG GAATCTATTT GAAGGCAGTT 
AATCTCACCA ATCATAATAG CCTCTTCACA AGATACAGTT ACTCTCTTTT TTGGGGGTTT 
CAGCAAATCA GCACGCTTGC TGGAAACCTA ATTCCAAGTT ACTTTCTTGG GGAGGTCTTC 
TTCACCATGG GCATTATCGG ATTGGGGCTT TTGCTTTTCG CTCTTCTTAT TGGTAATATG 
CAGAACTTCC TTCAATCTCT CGGTCGAAGG AATTTGGAAA TGACTTTGAG ACGGCGTGAT 
GTGGAGCAAT GGATGAGGCA TAGACGGTTG CCAGGAGATA TTAGAAAGAG GGTGCGAGAG 
GCTGAGCGGT TCAACTGGGC TGCTACCAGA GGAGTTAATG AAGAATTGTT GTTTAAGAAT 
ATGCCTGATG ACCTTCAAAG AGATATAAGA AAACACCTCT TCATATTTCT CAAGAAGGTG 
AGGATATTTT CGGTGATGGA TGAACCAATC TTAGATGCTA TACGTGAGAG GCTGAAACAA 
AGGACTTACA TAAGGAGTAG CATGGTCTTG CACCGCGGAG GTCTAGTTGA GAAAATGGTA 
TTCATAGTGA GAGGTGAGAT AGAGAGCGTT GGAGAAGATG GTTATGTTCT ACCATTGTCA 
GAAGGAGATG TTTGTGGTGA AGAACTTCTC ACTTGGTGCC TCGAACGCTC TTCTGTAAAC 
CCCGATGGGA CAAGGATACG GATGCCCTCA AAGGGACTGC ATAGCAACAG AAACGTTAGC 
TGTGTGACAA ACGTGGAGGC GTTTTCGCTG AGTGTAGCAG ATCTTGAAGA TGTAACGAGC 
TTGTTCTCTA GATTCCTAAG GAGCAATCGT GTGCAAGGAG CCATAAGGTA TGAGTCTCCT 
TATTGGAGGC TACGAGCAGC TATTCAGATT CAAGTGGCTT GGAGATACCG AAGGAGACGG 
CTTCAGAGAT TGTACACTGA TCAGTCTAGT TACAGCCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018514663.1
CDS	124..2385
Translation	MEKDDVPMLPVSDSSSLSRTRPFTSRSRSVSLANTSSILDGFDSSTVVLGYTGPLRTHRRPPLVQMSGPLSSTRSSEPLFLPPPPTSTRDVSSSQPERYPSFAALEHKNSEEEFVLKHAHLLRSGQLGMCNDPYCTTCPSYYNRKASQIPTSRVSAIFDSTFHDAKGWARRFATSINRHLPGIMNPHSKFVQRWTKFFAFSCLLAIFIDPLFFFLILVKQNNNCIEIDWPMAHTFVAVRSVTDVLFSVNILLQFRLAYVAPESTVVGAGQLVDHPRKVASHYFRGKFLLDLFIVMPLPQILILWIIPANLAISGANYAKNLLRAAVLFQYIPKLYRLLPLLAGQTPTGFIFESAWANFFINLLTFMLAGHVVGSCWYLFGLQRVNQCLRDACGSSDRPCRELIDCRGETSALSAAWKDNASARACFQEDGFPYGIYLKAVNLTNHNSLFTRYSYSLFWGFQQISTLAGNLIPSYFLGEVFFTMGIIGLGLLLFALLIGNMQNFLQSLGRRNLEMTLRRRDVEQWMRHRRLPGDIRKRVREAERFNWAATRGVNEELLFKNMPDDLQRDIRKHLFIFLKKVRIFSVMDEPILDAIRERLKQRTYIRSSMVLHRGGLVEKMVFIVRGEIESVGEDGYVLPLSEGDVCGEELLTWCLERSSVNPDGTRIRMPSKGLHSNRNVSCVTNVEAFSLSVADLEDVTSLFSRFLRSNRVQGAIRYESPYWRLRAAIQIQVAWRYRRRRLQRLYTDQSSYSL

Target ORF information:

RefSeq Version	XM_018659147.1
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa probable cyclic nucleotide-gated ion channel 20, chloroplastic (LOC103869811), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018659147.1

ORF Insert Sequence:

ATGGAGAAGG ATGATGTTCC CATGCTTCCA GTTTCAGACT CATCATCGTT ATCTCGTACT 
AGGCCTTTCA CTTCCAGGTC ACGTAGCGTT TCCCTCGCAA ACACTTCTTC GATCCTTGAT 
GGGTTCGACA GCTCGACTGT TGTTTTAGGC TACACAGGTC CTCTTCGAAC TCATAGAAGG 
CCTCCTTTAG TTCAGATGAG TGGTCCTCTC TCATCTACTC GCAGTTCTGA GCCTCTCTTT 
CTTCCTCCTC CTCCTACTTC CACTCGTGAT GTCTCCTCCT CTCAGCCTGA GAGGTACCCT 
TCCTTTGCTG CTCTCGAACA CAAAAACTCA GAGGAGGAGT TCGTATTGAA ACACGCACAT 
CTCTTGAGAT CTGGACAGTT GGGTATGTGT AATGATCCTT ACTGTACCAC TTGTCCTTCT 
TACTACAACC GGAAAGCTTC CCAAATCCCT ACTTCTAGAG TTTCTGCCAT TTTTGATTCC 
ACTTTCCATG ATGCTAAAGG TTGGGCTAGG AGGTTTGCTA CATCTATTAA TAGACACTTA 
CCAGGAATCA TGAATCCTCA CTCCAAGTTC GTTCAGAGAT GGACTAAGTT CTTTGCCTTT 
TCTTGCTTGT TAGCTATTTT TATAGATCCC CTCTTCTTCT TCCTCATATT AGTCAAGCAG 
AACAATAATT GCATTGAGAT TGATTGGCCG ATGGCTCACA CATTTGTAGC TGTTAGAAGT 
GTAACAGATG TTTTGTTCTC TGTAAACATC CTGCTTCAGT TCCGATTAGC CTATGTAGCT 
CCTGAGTCTA CGGTTGTTGG TGCTGGCCAG TTGGTAGACC ATCCAAGAAA AGTTGCTAGT 
CATTACTTCC GAGGAAAGTT TTTACTAGAT TTGTTCATAG TGATGCCACT TCCACAGATA 
TTGATATTAT GGATAATACC AGCAAACTTA GCCATATCTG GGGCAAACTA TGCAAAGAAC 
CTTTTACGTG CTGCAGTTCT TTTCCAATAC ATTCCCAAGT TATATAGGCT ACTACCACTT 
CTTGCTGGAC AAACACCGAC TGGCTTCATC TTTGAGTCGG CTTGGGCTAA TTTTTTTATT 
AATCTTCTTA CCTTCATGCT TGCTGGTCAT GTCGTTGGCT CTTGTTGGTA TCTTTTTGGT 
CTGCAGAGAG TTAATCAATG CCTTCGAGAT GCTTGCGGTA GTTCTGATCG TCCATGCAGA 
GAGCTTATAG ATTGTCGTGG TGAAACTAGC GCTTTATCAG CTGCGTGGAA AGATAATGCG 
AGTGCCCGTG CTTGTTTTCA AGAGGATGGT TTTCCTTATG GAATCTATTT GAAGGCAGTT 
AATCTCACCA ATCATAATAG CCTCTTCACA AGATACAGTT ACTCTCTTTT TTGGGGGTTT 
CAGCAAATCA GCACGCTTGC TGGAAACCTA ATTCCAAGTT ACTTTCTTGG GGAGGTCTTC 
TTCACCATGG GCATTATCGG ATTGGGGCTT TTGCTTTTCG CTCTTCTTAT TGGTAATATG 
CAGAACTTCC TTCAATCTCT CGGTCGAAGG AATTTGGAAA TGACTTTGAG ACGGCGTGAT 
GTGGAGCAAT GGATGAGGCA TAGACGGTTG CCAGGAGATA TTAGAAAGAG GGTGCGAGAG 
GCTGAGCGGT TCAACTGGGC TGCTACCAGA GGAGTTAATG AAGAATTGTT GTTTAAGAAT 
ATGCCTGATG ACCTTCAAAG AGATATAAGA AAACACCTCT TCATATTTCT CAAGAAGGTG 
AGGATATTTT CGGTGATGGA TGAACCAATC TTAGATGCTA TACGTGAGAG GCTGAAACAA 
AGGACTTACA TAAGGAGTAG CATGGTCTTG CACCGCGGAG GTCTAGTTGA GAAAATGGTA 
TTCATAGTGA GAGGTGAGAT AGAGAGCGTT GGAGAAGATG GTTATGTTCT ACCATTGTCA 
GAAGGAGATG TTTGTGGTGA AGAACTTCTC ACTTGGTGCC TCGAACGCTC TTCTGTAAAC 
CCCGATGGGA CAAGGATACG GATGCCCTCA AAGGGACTGC ATAGCAACAG AAACGTTAGC 
TGTGTGACAA ACGTGGAGGC GTTTTCGCTG AGTGTAGCAG ATCTTGAAGA TGTAACGAGC 
TTGTTCTCTA GATTCCTAAG GAGCAATCGT GTGCAAGGAG CCATAAGGTA TGAGTCTCCT 
TATTGGAGGC TACGAGCAGC TATTCAGATT CAAGTGGCTT GGAGATACCG AAGGAGACGG 
CTTCAGAGAT TGTACACTGA TCAGTCTAGT TACAGCCTTT AG