VPS8 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015472150.1	XP_015327636.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_005201567.3	XP_005201624.1	vacuolar protein sorting-associated protein 8 homolog isoform X3
XM_015472153.1	XP_015327639.1	vacuolar protein sorting-associated protein 8 homolog isoform X4
XM_015472149.1	XP_015327635.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_024995290.1	XP_024851058.1	vacuolar protein sorting-associated protein 8 homolog isoform X4
XM_015462056.1	XP_015317542.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_015462058.1	XP_015317544.1	vacuolar protein sorting-associated protein 8 homolog isoform X4
XM_024995278.1	XP_024851046.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_024995281.1	XP_024851049.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_001251842.7	XP_001251843.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_015462057.1	XP_015317543.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_024995284.1	XP_024851052.1	vacuolar protein sorting-associated protein 8 homolog isoform X3
XM_005192219.3	XP_005192276.1	vacuolar protein sorting-associated protein 8 homolog isoform X3
XM_002684849.5	XP_002684895.1	vacuolar protein sorting-associated protein 8 homolog isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219165	XM_024995278.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219166	XM_024995281.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219167	XM_024995284.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa219168	XM_024995290.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa87754	XM_015462058.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa87754	XM_015472153.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa15784	XM_015472149.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15784	XM_002684849.5 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15784	XM_015462056.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15784	XM_001251842.7 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15785	XM_015462057.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15785	XM_015472150.1 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15786	XM_005192219.3 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa15786	XM_005201567.3 Latest version!	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

VPS8 ( XM_024995278.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851046.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219165
Clone ID Related Accession (Same CDS sequence)	XM_024995278.1
Accession Version	XM_024995278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAGGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACAAATG CAGCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851046.1
CDS	225..4505
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYKCSSSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_024995278.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995278.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAGGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACAAATG CAGCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_024995281.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851049.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219166
Clone ID Related Accession (Same CDS sequence)	XM_024995281.1
Accession Version	XM_024995281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAGGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACAAATG CAGCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851049.1
CDS	225..4451
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYKCSSSNKAGKLSENSSEVKKGRITPSQATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_024995281.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995281.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAGGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACAAATG CAGCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_024995284.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851052.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

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CloneID	OBa219167
Clone ID Related Accession (Same CDS sequence)	XM_024995284.1
Accession Version	XM_024995284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACATT CACTTTTGAA GGGAATTTCT GCCCAGATAG TGTCTGCAGC TGACAAAGTA 
GATGCTGGCT TGCCTACAGC AATTGCAGTA TCCAGTTTGA TAGCAGTGGG TACATCTCAT 
GGATTGGCCT TAATATTTGA TCAGAATCAA GCTTTGCGAC TCTGCTTGGG TAGCACTAGT 
GTTGGAGGTC AGTATGGAGC TATCTCTGCC CTCAGTATCA ACAATGACTG CTCAAGACTT 
CTCTGTGGCT TTGCTAAGGG ACAGATCACT ATGTGGGATT TGGCCAGTGG AAAGCTTCTA 
AGATCAATAA CAGATGCTCA TCCTCCGGGA ACAGCAATAT TACATATTAA GTTTACAGAT 
GATCCAACTC TTGCAATTTG CAATGACAGC GGAGGCTCTG TTTTTGAATT GACATTTAAG 
AGAGTGATGG GAGTGAGAAC TTGTGAATCT CGATGCCTGT TTAGCGGTTC CAAGGGTGAA 
GTTTGCTGTA TTGAACCTCT CCACTCTAAG CCCGAGTTGA AAGACCATCC CATCACACAA 
TTTTCCTTAT TGGCCATGGC ATCCTTAACA AAGATACTGG TCATTGGATT GAAGCCATCC 
TTGAAAGTGT GGATGACTTT TCCTTATGGA CGGATGGATC CTTCCAGCGT TCCATTGCTG 
GCCTGGCACT TCGTGGCAGT GCATAATTAC GTGAATCCCA TGCTAGCCTT CTGCAGAGGA 
GATGTTGTTC ATTTTCTCCT GGTTAAGAGA GACGAATCTG GAGCGATACA TGTTACTAAG 
CAAAAGCATC TTCACCTATA CTATGACCTC ATCAACTTTA CTTGGATAAA CTCACGCACA 
ATTGTGCTCT TGGACAGTGT AGAGAAGTTG CATGTGATTG ATCGGCAAAC TCAGGAGGAA 
CTGGAAACAG TGGAGATCTC AGAAGTTCAG TTGGTCTATA ATAGCAGCCA TTTCAAATCA 
CTAGCCACTG GAGGAAACGT GAGCCAGGCA CTGGCTTTGG TTGGAGAGAA GGCTTGCTAT 
CAATCCATAA GTAGCTATGG TGGTCAGATC TTTTATTTGG GAACAAAATC TGTTTATGTG 
ATGATGCTGA GGAGCTGGAG GGAGAGAGTG GATCACCTCT TGAAACAAGA CTGTCTTACA 
GAAGCTTTGG CTCTGGCATG GTCTTTCCAT GAAGGAAAAG CAAAAGCAGT AGTAGGGTTA 
TCAGGGGATG CCAGTAAGCG AAAGGCTATT GTTGCAGATC GGATGGTAGA AATCCTATTG 
CATTATGCAG ACCGAGCTCT AAAAAAGTGC CCAGACCAGG GAAAAATCCA AGTGATGGAG 
CAACATTTTC AGGATATGGT ACCCGTCATA GTTGATTACT GCCTTCTGCT ACAGCGAAAG 
GATCTTTTAT TTAGCCAGGT GTATGACAAA TTAAATGAGA ATTCAGTGGC CAAAGGAGTG 
TTTCTGGAGT GCCTTGAACC ATATATTTTA AGTGATAAAT TGGTGGGGAT CACACCCCAG 
GTGATGAAAG ACTTGATTGT TCATTTCCAA GATAAAAAGT TAATGGAAAA TGTGGAAGCC 
CTCATTGTGC ATATGGATAT CACCAGCCTA GATATTCAGC AGATAGTTCT CATGTGTTGG 
GAAAATCGAC TATATGATGC TATGATCTAT GTCTACAACA GAGGCATGAA TGAATTTATT 
AGTCCAATGG AGAAACTTTT CAGAGTCATT GCACCTCCTT TGAATGCAGG AAAGACGCTA 
ACAGATGAGC AAGTTGTTAT GGGCAACAAG CTTCTTGTAT ATATTAGCTG TTGTCTAGCA 
GGTCGTGCCT ATCCGCTGGG TGACATCCCT GAAGATCTTG TTCCCTTGGT TAAAAACCAG 
GTTTTTGAAT TTCTAATTCG CCTGCATTCA ACAGAAGCTC CCCCTGAAGA AGAAATCTAT 
CCTTATATTC GGACTTTGCT GCATTTCGAC ACACGAGAAT TTCTAAATGT ATTGGCGCTG 
ACTTTTGAAG ATTTTAAAAA TGATAAGCAA GCTGTAGAAT ATCAACAACG TATTGTGGAT 
ATTTTGTTGA AGGTTATGGT GGAGAATTCA GATTTTACGC CCTCACAAGT GGGGTGTCTC 
TTTACCTTCC TTGCCCGGCA GCTTGCGAAG CCTGACAACA CCTTATTTGT AAACAGAACT 
CTTTTTGATC AGGTCCTTGA ATTCCTTTGT AGTCCTGATG ATGACTCCCG ACACTCTGAA 
AGACAGCAGG TCCTTTTAGA ATTGCTGCAG GCTGGAGGCA TAGTTCAGTT TGAAGAGAGT 
CGACTCATCC GTATGGCAGA GAAAGCAGAA TTCTACCAAA TTTGTGAATT TATGTATGAA 
CAAGAACACC AATATGACAG AATTATTGAT TGCTACTTAC GTGACCCACT ACGAGAGGAA 
GAAGTCTTTA ACTACATTCA CAATATCTTA TCCATTCCTG GATACAGTGT GGAGGAGAAG 
CAGTCTGTAT GGCAGAAGGC TATGGATCAT ATTGAGGAAC TTGTGGCCCT GAAGCCCTGT 
AAAGCTGCAG AGCTGGTTGC TACTCACTTT TCTGAACAGA TCGAAACAGT CATTAAGAAA 
CTTCAGAACC AGGTTTTGCT TTTCAAGTTT TTAAGAAGCC TTCTTGACCC AAGGGAAGGT 
ATTCATGTAA ATCAAGAATC GCTGCAGATA TCTCCCTGTA TAATAGAGCA GTTCATTGAG 
CTCTTGTGTC AGTTCAGCCC AAACCACGTT ATTGAGACGC TGCAAGTCCT TGAGTGCTAC 
CGCCTAGAAG AAACTATTCA GATTACCCAG AAGTATCAAC TTTGTGAAGT CACTGCTTAT 
CTATTAGAAA AGAAAGGAGA TATTCATGGT GCCTTCTTAA TAATGCTAAA GAGACTACAA 
AGCAAACTTC AAGAGATAAT ACGTCAAGAT AAAAATACCA AAGAGGATCC CTCATTGAAG 
GATGTTGAAG AGACCATGGT AGAGACGATT GCCCTCTGCC AGAGAAATTC ACATAATTTG 
AACCAACAGC AGAGAGAGGC ACTCTGGTTT CCGTTATTGG AGGCAATGAT GGCCCCTCAG 
AAGCTGTCTA GTTCAGCTTC ACCTCATCCA CACTCTGAAG CTCTGAAATC TTTGACCATG 
CAAGTTCTCA ACAGTATGGC AGCATTTATT GCCCTTCCAT CCATCCTGCA GCGAATCTTA 
CAGGACCCAG TTTATGGAAA AGGAAAACTT GGAGAAATCC AGGGCCTTAT TCTGGGAATG 
TTGGATACCT TTAACTATGA ACAGACCCTG CTGGAAACGA CAACTAGCCT TCTAAACCAA 
GATCTCCATT GGTCATTGTG TAACCTGAGA GCTTCAGTTA CCAGAGGACT GAATCCCAAG 
CAGGATTACT GCTCTATTTG TTTGCAGCAG TACAAGAGAC GCCAAGAAAT GGCTGATGAA 
ATTATTGTCT TTAGCTGTGG CCATTTATAT CACTCGTTCT GTCTGCAAAG CAAAGAGTGC 
ACCATAGAAA TTGAAGGGCA AACAAGATGG ACGTGCTACA AATGCAGCTC AAGTAACAAA 
GCAGGAAAGT TGAGTGAAAA TTCATCTGAA GTTAAAAAGG GAAGGATAAC CCCATCACAG 
GTAAAAATGT CCCCATCCTG TCATCTGTCC AAAGGGGATC CTGCTATTAA AAAGGCAACC 
TCAGAACCTG TTCTGGATCC ACAGCAAATC CAAGCATTTG ATCAGCTCTG CCATCTCTAC 
CGAGGAAGTT CCAGGCTGGC CCTCCTCACG GAACTCTCCC AGAATCGCAG TGGCGAGAGC 
TGCAGGCCAT TTGGAGGGTC CCAGAGTGGC CCCGCCTTCA ACAGCATCTT CCAGAAGGAG 
AACTTCCAGC TGCAGCTCAT TCCTCCCCCG GTGACTGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851052.1
CDS	261..4145
Translation	MRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYKCSSSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_024995284.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995284.1

ORF Insert Sequence:

ATGCGACATT CACTTTTGAA GGGAATTTCT GCCCAGATAG TGTCTGCAGC TGACAAAGTA 
GATGCTGGCT TGCCTACAGC AATTGCAGTA TCCAGTTTGA TAGCAGTGGG TACATCTCAT 
GGATTGGCCT TAATATTTGA TCAGAATCAA GCTTTGCGAC TCTGCTTGGG TAGCACTAGT 
GTTGGAGGTC AGTATGGAGC TATCTCTGCC CTCAGTATCA ACAATGACTG CTCAAGACTT 
CTCTGTGGCT TTGCTAAGGG ACAGATCACT ATGTGGGATT TGGCCAGTGG AAAGCTTCTA 
AGATCAATAA CAGATGCTCA TCCTCCGGGA ACAGCAATAT TACATATTAA GTTTACAGAT 
GATCCAACTC TTGCAATTTG CAATGACAGC GGAGGCTCTG TTTTTGAATT GACATTTAAG 
AGAGTGATGG GAGTGAGAAC TTGTGAATCT CGATGCCTGT TTAGCGGTTC CAAGGGTGAA 
GTTTGCTGTA TTGAACCTCT CCACTCTAAG CCCGAGTTGA AAGACCATCC CATCACACAA 
TTTTCCTTAT TGGCCATGGC ATCCTTAACA AAGATACTGG TCATTGGATT GAAGCCATCC 
TTGAAAGTGT GGATGACTTT TCCTTATGGA CGGATGGATC CTTCCAGCGT TCCATTGCTG 
GCCTGGCACT TCGTGGCAGT GCATAATTAC GTGAATCCCA TGCTAGCCTT CTGCAGAGGA 
GATGTTGTTC ATTTTCTCCT GGTTAAGAGA GACGAATCTG GAGCGATACA TGTTACTAAG 
CAAAAGCATC TTCACCTATA CTATGACCTC ATCAACTTTA CTTGGATAAA CTCACGCACA 
ATTGTGCTCT TGGACAGTGT AGAGAAGTTG CATGTGATTG ATCGGCAAAC TCAGGAGGAA 
CTGGAAACAG TGGAGATCTC AGAAGTTCAG TTGGTCTATA ATAGCAGCCA TTTCAAATCA 
CTAGCCACTG GAGGAAACGT GAGCCAGGCA CTGGCTTTGG TTGGAGAGAA GGCTTGCTAT 
CAATCCATAA GTAGCTATGG TGGTCAGATC TTTTATTTGG GAACAAAATC TGTTTATGTG 
ATGATGCTGA GGAGCTGGAG GGAGAGAGTG GATCACCTCT TGAAACAAGA CTGTCTTACA 
GAAGCTTTGG CTCTGGCATG GTCTTTCCAT GAAGGAAAAG CAAAAGCAGT AGTAGGGTTA 
TCAGGGGATG CCAGTAAGCG AAAGGCTATT GTTGCAGATC GGATGGTAGA AATCCTATTG 
CATTATGCAG ACCGAGCTCT AAAAAAGTGC CCAGACCAGG GAAAAATCCA AGTGATGGAG 
CAACATTTTC AGGATATGGT ACCCGTCATA GTTGATTACT GCCTTCTGCT ACAGCGAAAG 
GATCTTTTAT TTAGCCAGGT GTATGACAAA TTAAATGAGA ATTCAGTGGC CAAAGGAGTG 
TTTCTGGAGT GCCTTGAACC ATATATTTTA AGTGATAAAT TGGTGGGGAT CACACCCCAG 
GTGATGAAAG ACTTGATTGT TCATTTCCAA GATAAAAAGT TAATGGAAAA TGTGGAAGCC 
CTCATTGTGC ATATGGATAT CACCAGCCTA GATATTCAGC AGATAGTTCT CATGTGTTGG 
GAAAATCGAC TATATGATGC TATGATCTAT GTCTACAACA GAGGCATGAA TGAATTTATT 
AGTCCAATGG AGAAACTTTT CAGAGTCATT GCACCTCCTT TGAATGCAGG AAAGACGCTA 
ACAGATGAGC AAGTTGTTAT GGGCAACAAG CTTCTTGTAT ATATTAGCTG TTGTCTAGCA 
GGTCGTGCCT ATCCGCTGGG TGACATCCCT GAAGATCTTG TTCCCTTGGT TAAAAACCAG 
GTTTTTGAAT TTCTAATTCG CCTGCATTCA ACAGAAGCTC CCCCTGAAGA AGAAATCTAT 
CCTTATATTC GGACTTTGCT GCATTTCGAC ACACGAGAAT TTCTAAATGT ATTGGCGCTG 
ACTTTTGAAG ATTTTAAAAA TGATAAGCAA GCTGTAGAAT ATCAACAACG TATTGTGGAT 
ATTTTGTTGA AGGTTATGGT GGAGAATTCA GATTTTACGC CCTCACAAGT GGGGTGTCTC 
TTTACCTTCC TTGCCCGGCA GCTTGCGAAG CCTGACAACA CCTTATTTGT AAACAGAACT 
CTTTTTGATC AGGTCCTTGA ATTCCTTTGT AGTCCTGATG ATGACTCCCG ACACTCTGAA 
AGACAGCAGG TCCTTTTAGA ATTGCTGCAG GCTGGAGGCA TAGTTCAGTT TGAAGAGAGT 
CGACTCATCC GTATGGCAGA GAAAGCAGAA TTCTACCAAA TTTGTGAATT TATGTATGAA 
CAAGAACACC AATATGACAG AATTATTGAT TGCTACTTAC GTGACCCACT ACGAGAGGAA 
GAAGTCTTTA ACTACATTCA CAATATCTTA TCCATTCCTG GATACAGTGT GGAGGAGAAG 
CAGTCTGTAT GGCAGAAGGC TATGGATCAT ATTGAGGAAC TTGTGGCCCT GAAGCCCTGT 
AAAGCTGCAG AGCTGGTTGC TACTCACTTT TCTGAACAGA TCGAAACAGT CATTAAGAAA 
CTTCAGAACC AGGTTTTGCT TTTCAAGTTT TTAAGAAGCC TTCTTGACCC AAGGGAAGGT 
ATTCATGTAA ATCAAGAATC GCTGCAGATA TCTCCCTGTA TAATAGAGCA GTTCATTGAG 
CTCTTGTGTC AGTTCAGCCC AAACCACGTT ATTGAGACGC TGCAAGTCCT TGAGTGCTAC 
CGCCTAGAAG AAACTATTCA GATTACCCAG AAGTATCAAC TTTGTGAAGT CACTGCTTAT 
CTATTAGAAA AGAAAGGAGA TATTCATGGT GCCTTCTTAA TAATGCTAAA GAGACTACAA 
AGCAAACTTC AAGAGATAAT ACGTCAAGAT AAAAATACCA AAGAGGATCC CTCATTGAAG 
GATGTTGAAG AGACCATGGT AGAGACGATT GCCCTCTGCC AGAGAAATTC ACATAATTTG 
AACCAACAGC AGAGAGAGGC ACTCTGGTTT CCGTTATTGG AGGCAATGAT GGCCCCTCAG 
AAGCTGTCTA GTTCAGCTTC ACCTCATCCA CACTCTGAAG CTCTGAAATC TTTGACCATG 
CAAGTTCTCA ACAGTATGGC AGCATTTATT GCCCTTCCAT CCATCCTGCA GCGAATCTTA 
CAGGACCCAG TTTATGGAAA AGGAAAACTT GGAGAAATCC AGGGCCTTAT TCTGGGAATG 
TTGGATACCT TTAACTATGA ACAGACCCTG CTGGAAACGA CAACTAGCCT TCTAAACCAA 
GATCTCCATT GGTCATTGTG TAACCTGAGA GCTTCAGTTA CCAGAGGACT GAATCCCAAG 
CAGGATTACT GCTCTATTTG TTTGCAGCAG TACAAGAGAC GCCAAGAAAT GGCTGATGAA 
ATTATTGTCT TTAGCTGTGG CCATTTATAT CACTCGTTCT GTCTGCAAAG CAAAGAGTGC 
ACCATAGAAA TTGAAGGGCA AACAAGATGG ACGTGCTACA AATGCAGCTC AAGTAACAAA 
GCAGGAAAGT TGAGTGAAAA TTCATCTGAA GTTAAAAAGG GAAGGATAAC CCCATCACAG 
GTAAAAATGT CCCCATCCTG TCATCTGTCC AAAGGGGATC CTGCTATTAA AAAGGCAACC 
TCAGAACCTG TTCTGGATCC ACAGCAAATC CAAGCATTTG ATCAGCTCTG CCATCTCTAC 
CGAGGAAGTT CCAGGCTGGC CCTCCTCACG GAACTCTCCC AGAATCGCAG TGGCGAGAGC 
TGCAGGCCAT TTGGAGGGTC CCAGAGTGGC CCCGCCTTCA ACAGCATCTT CCAGAAGGAG 
AACTTCCAGC TGCAGCTCAT TCCTCCCCCG GTGACTGAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_024995290.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851058.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

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CloneID	OBa219168
Clone ID Related Accession (Same CDS sequence)	XM_024995290.1
Accession Version	XM_024995290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCC TCCGGGAACA GCAATATTAC ATATTAAGGA GAGTGATGGG AGTGAGAACT 
TGTGAATCTC GATGCCTGTT TAGCGGTTCC AAGGGTGAAG TTTGCTGTAT TGAACCTCTC 
CACTCTAAGC CCGAGTTGAA AGACCATCCC ATCACACAAT TTTCCTTATT GGCCATGGCA 
TCCTTAACAA AGATACTGGT CATTGGATTG AAGCCATCCT TGAAAGTGTG GATGACTTTT 
CCTTATGGAC GGATGGATCC TTCCAGCGTT CCATTGCTGG CCTGGCACTT CGTGGCAGTG 
CATAATTACG TGAATCCCAT GCTAGCCTTC TGCAGAGGAG ATGTTGTTCA TTTTCTCCTG 
GTTAAGAGAG ACGAATCTGG AGCGATACAT GTTACTAAGC AAAAGCATCT TCACCTATAC 
TATGACCTCA TCAACTTTAC TTGGATAAAC TCACGCACAA TTGTGCTCTT GGACAGTGTA 
GAGAAGTTGC ATGTGATTGA TCGGCAAACT CAGGAGGAAC TGGAAACAGT GGAGATCTCA 
GAAGTTCAGT TGGTCTATAA TAGCAGCCAT TTCAAATCAC TAGCCACTGG AGGAAACGTG 
AGCCAGGCAC TGGCTTTGGT TGGAGAGAAG GCTTGCTATC AATCCATAAG TAGCTATGGT 
GGTCAGATCT TTTATTTGGG AACAAAATCT GTTTATGTGA TGATGCTGAG GAGCTGGAGG 
GAGAGAGTGG ATCACCTCTT GAAACAAGAC TGTCTTACAG AAGCTTTGGC TCTGGCATGG 
TCTTTCCATG AAGGAAAAGC AAAAGCAGTA GTAGGGTTAT CAGGGGATGC CAGTAAGCGA 
AAGGCTATTG TTGCAGATCG GATGGTAGAA ATCCTATTGC ATTATGCAGA CCGAGCTCTA 
AAAAAGTGCC CAGACCAGGG AAAAATCCAA GTGATGGAGC AACATTTTCA GGATATGGTA 
CCCGTCATAG TTGATTACTG CCTTCTGCTA CAGCGAAAGG ATCTTTTATT TAGCCAGGTG 
TATGACAAAT TAAATGAGAA TTCAGTGGCC AAAGGAGTGT TTCTGGAGTG CCTTGAACCA 
TATATTTTAA GTGATAAATT GGTGGGGATC ACACCCCAGG TGATGAAAGA CTTGATTGTT 
CATTTCCAAG ATAAAAAGTT AATGGAAAAT GTGGAAGCCC TCATTGTGCA TATGGATATC 
ACCAGCCTAG ATATTCAGCA GATAGTTCTC ATGTGTTGGG AAAATCGACT ATATGATGCT 
ATGATCTATG TCTACAACAG AGGCATGAAT GAATTTATTA GTCCAATGGA GAAACTTTTC 
AGAGTCATTG CACCTCCTTT GAATGCAGGA AAGACGCTAA CAGATGAGCA AGTTGTTATG 
GGCAACAAGC TTCTTGTATA TATTAGCTGT TGTCTAGCAG GTCGTGCCTA TCCGCTGGGT 
GACATCCCTG AAGATCTTGT TCCCTTGGTT AAAAACCAGG TTTTTGAATT TCTAATTCGC 
CTGCATTCAA CAGAAGCTCC CCCTGAAGAA GAAATCTATC CTTATATTCG GACTTTGCTG 
CATTTCGACA CACGAGAATT TCTAAATGTA TTGGCGCTGA CTTTTGAAGA TTTTAAAAAT 
GATAAGCAAG CTGTAGAATA TCAACAACGT ATTGTGGATA TTTTGTTGAA GGTTATGGTG 
GAGAATTCAG ATTTTACGCC CTCACAAGTG GGGTGTCTCT TTACCTTCCT TGCCCGGCAG 
CTTGCGAAGC CTGACAACAC CTTATTTGTA AACAGAACTC TTTTTGATCA GGTCCTTGAA 
TTCCTTTGTA GTCCTGATGA TGACTCCCGA CACTCTGAAA GACAGCAGGT CCTTTTAGAA 
TTGCTGCAGG CTGGAGGCAT AGTTCAGTTT GAAGAGAGTC GACTCATCCG TATGGCAGAG 
AAAGCAGAAT TCTACCAAAT TTGTGAATTT ATGTATGAAC AAGAACACCA ATATGACAGA 
ATTATTGATT GCTACTTACG TGACCCACTA CGAGAGGAAG AAGTCTTTAA CTACATTCAC 
AATATCTTAT CCATTCCTGG ATACAGTGTG GAGGAGAAGC AGTCTGTATG GCAGAAGGCT 
ATGGATCATA TTGAGGAACT TGTGGCCCTG AAGCCCTGTA AAGCTGCAGA GCTGGTTGCT 
ACTCACTTTT CTGAACAGAT CGAAACAGTC ATTAAGAAAC TTCAGAACCA GGTTTTGCTT 
TTCAAGTTTT TAAGAAGCCT TCTTGACCCA AGGGAAGGTA TTCATGTAAA TCAAGAATCG 
CTGCAGATAT CTCCCTGTAT AATAGAGCAG TTCATTGAGC TCTTGTGTCA GTTCAGCCCA 
AACCACGTTA TTGAGACGCT GCAAGTCCTT GAGTGCTACC GCCTAGAAGA AACTATTCAG 
ATTACCCAGA AGTATCAACT TTGTGAAGTC ACTGCTTATC TATTAGAAAA GAAAGGAGAT 
ATTCATGGTG CCTTCTTAAT AATGCTAAAG AGACTACAAA GCAAACTTCA AGAGATAATA 
CGTCAAGATA AAAATACCAA AGAGGATCCC TCATTGAAGG ATGTTGAAGA GACCATGGTA 
GAGACGATTG CCCTCTGCCA GAGAAATTCA CATAATTTGA ACCAACAGCA GAGAGAGGCA 
CTCTGGTTTC CGTTATTGGA GGCAATGATG GCCCCTCAGA AGCTGTCTAG TTCAGCTTCA 
CCTCATCCAC ACTCTGAAGC TCTGAAATCT TTGACCATGC AAGTTCTCAA CAGTATGGCA 
GCATTTATTG CCCTTCCATC CATCCTGCAG CGAATCTTAC AGGACCCAGT TTATGGAAAA 
GGAAAACTTG GAGAAATCCA GGGCCTTATT CTGGGAATGT TGGATACCTT TAACTATGAA 
CAGACCCTGC TGGAAACGAC AACTAGCCTT CTAAACCAAG ATCTCCATTG GTCATTGTGT 
AACCTGAGAG CTTCAGTTAC CAGAGGACTG AATCCCAAGC AGGATTACTG CTCTATTTGT 
TTGCAGCAGT ACAAGAGACG CCAAGAAATG GCTGATGAAA TTATTGTCTT TAGCTGTGGC 
CATTTATATC ACTCGTTCTG TCTGCAAAGC AAAGAGTGCA CCATAGAAAT TGAAGGGCAA 
ACAAGATGGA CGTGCTACAA ATGCAGCTCA AGTAACAAAG CAGGAAAGTT GAGTGAAAAT 
TCATCTGAAG TTAAAAAGGG AAGGATAACC CCATCACAGG TAAAAATGTC CCCATCCTGT 
CATCTGTCCA AAGGGGATCC TGCTATTAAA AAGGCAACCT CAGAACCTGT TCTGGATCCA 
CAGCAAATCC AAGCATTTGA TCAGCTCTGC CATCTCTACC GAGGAAGTTC CAGGCTGGCC 
CTCCTCACGG AACTCTCCCA GAATCGCAGT GGCGAGAGCT GCAGGCCATT TGGAGGGTCC 
CAGAGTGGCC CCGCCTTCAA CAGCATCTTC CAGAAGGAGA ACTTCCAGCT GCAGCTCATT 
CCTCCCCCGG TGACTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851058.1
CDS	175..3678
Translation	MLILREQQYYILRRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYKCSSSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_024995290.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995290.1

ORF Insert Sequence:

ATGCTCATCC TCCGGGAACA GCAATATTAC ATATTAAGGA GAGTGATGGG AGTGAGAACT 
TGTGAATCTC GATGCCTGTT TAGCGGTTCC AAGGGTGAAG TTTGCTGTAT TGAACCTCTC 
CACTCTAAGC CCGAGTTGAA AGACCATCCC ATCACACAAT TTTCCTTATT GGCCATGGCA 
TCCTTAACAA AGATACTGGT CATTGGATTG AAGCCATCCT TGAAAGTGTG GATGACTTTT 
CCTTATGGAC GGATGGATCC TTCCAGCGTT CCATTGCTGG CCTGGCACTT CGTGGCAGTG 
CATAATTACG TGAATCCCAT GCTAGCCTTC TGCAGAGGAG ATGTTGTTCA TTTTCTCCTG 
GTTAAGAGAG ACGAATCTGG AGCGATACAT GTTACTAAGC AAAAGCATCT TCACCTATAC 
TATGACCTCA TCAACTTTAC TTGGATAAAC TCACGCACAA TTGTGCTCTT GGACAGTGTA 
GAGAAGTTGC ATGTGATTGA TCGGCAAACT CAGGAGGAAC TGGAAACAGT GGAGATCTCA 
GAAGTTCAGT TGGTCTATAA TAGCAGCCAT TTCAAATCAC TAGCCACTGG AGGAAACGTG 
AGCCAGGCAC TGGCTTTGGT TGGAGAGAAG GCTTGCTATC AATCCATAAG TAGCTATGGT 
GGTCAGATCT TTTATTTGGG AACAAAATCT GTTTATGTGA TGATGCTGAG GAGCTGGAGG 
GAGAGAGTGG ATCACCTCTT GAAACAAGAC TGTCTTACAG AAGCTTTGGC TCTGGCATGG 
TCTTTCCATG AAGGAAAAGC AAAAGCAGTA GTAGGGTTAT CAGGGGATGC CAGTAAGCGA 
AAGGCTATTG TTGCAGATCG GATGGTAGAA ATCCTATTGC ATTATGCAGA CCGAGCTCTA 
AAAAAGTGCC CAGACCAGGG AAAAATCCAA GTGATGGAGC AACATTTTCA GGATATGGTA 
CCCGTCATAG TTGATTACTG CCTTCTGCTA CAGCGAAAGG ATCTTTTATT TAGCCAGGTG 
TATGACAAAT TAAATGAGAA TTCAGTGGCC AAAGGAGTGT TTCTGGAGTG CCTTGAACCA 
TATATTTTAA GTGATAAATT GGTGGGGATC ACACCCCAGG TGATGAAAGA CTTGATTGTT 
CATTTCCAAG ATAAAAAGTT AATGGAAAAT GTGGAAGCCC TCATTGTGCA TATGGATATC 
ACCAGCCTAG ATATTCAGCA GATAGTTCTC ATGTGTTGGG AAAATCGACT ATATGATGCT 
ATGATCTATG TCTACAACAG AGGCATGAAT GAATTTATTA GTCCAATGGA GAAACTTTTC 
AGAGTCATTG CACCTCCTTT GAATGCAGGA AAGACGCTAA CAGATGAGCA AGTTGTTATG 
GGCAACAAGC TTCTTGTATA TATTAGCTGT TGTCTAGCAG GTCGTGCCTA TCCGCTGGGT 
GACATCCCTG AAGATCTTGT TCCCTTGGTT AAAAACCAGG TTTTTGAATT TCTAATTCGC 
CTGCATTCAA CAGAAGCTCC CCCTGAAGAA GAAATCTATC CTTATATTCG GACTTTGCTG 
CATTTCGACA CACGAGAATT TCTAAATGTA TTGGCGCTGA CTTTTGAAGA TTTTAAAAAT 
GATAAGCAAG CTGTAGAATA TCAACAACGT ATTGTGGATA TTTTGTTGAA GGTTATGGTG 
GAGAATTCAG ATTTTACGCC CTCACAAGTG GGGTGTCTCT TTACCTTCCT TGCCCGGCAG 
CTTGCGAAGC CTGACAACAC CTTATTTGTA AACAGAACTC TTTTTGATCA GGTCCTTGAA 
TTCCTTTGTA GTCCTGATGA TGACTCCCGA CACTCTGAAA GACAGCAGGT CCTTTTAGAA 
TTGCTGCAGG CTGGAGGCAT AGTTCAGTTT GAAGAGAGTC GACTCATCCG TATGGCAGAG 
AAAGCAGAAT TCTACCAAAT TTGTGAATTT ATGTATGAAC AAGAACACCA ATATGACAGA 
ATTATTGATT GCTACTTACG TGACCCACTA CGAGAGGAAG AAGTCTTTAA CTACATTCAC 
AATATCTTAT CCATTCCTGG ATACAGTGTG GAGGAGAAGC AGTCTGTATG GCAGAAGGCT 
ATGGATCATA TTGAGGAACT TGTGGCCCTG AAGCCCTGTA AAGCTGCAGA GCTGGTTGCT 
ACTCACTTTT CTGAACAGAT CGAAACAGTC ATTAAGAAAC TTCAGAACCA GGTTTTGCTT 
TTCAAGTTTT TAAGAAGCCT TCTTGACCCA AGGGAAGGTA TTCATGTAAA TCAAGAATCG 
CTGCAGATAT CTCCCTGTAT AATAGAGCAG TTCATTGAGC TCTTGTGTCA GTTCAGCCCA 
AACCACGTTA TTGAGACGCT GCAAGTCCTT GAGTGCTACC GCCTAGAAGA AACTATTCAG 
ATTACCCAGA AGTATCAACT TTGTGAAGTC ACTGCTTATC TATTAGAAAA GAAAGGAGAT 
ATTCATGGTG CCTTCTTAAT AATGCTAAAG AGACTACAAA GCAAACTTCA AGAGATAATA 
CGTCAAGATA AAAATACCAA AGAGGATCCC TCATTGAAGG ATGTTGAAGA GACCATGGTA 
GAGACGATTG CCCTCTGCCA GAGAAATTCA CATAATTTGA ACCAACAGCA GAGAGAGGCA 
CTCTGGTTTC CGTTATTGGA GGCAATGATG GCCCCTCAGA AGCTGTCTAG TTCAGCTTCA 
CCTCATCCAC ACTCTGAAGC TCTGAAATCT TTGACCATGC AAGTTCTCAA CAGTATGGCA 
GCATTTATTG CCCTTCCATC CATCCTGCAG CGAATCTTAC AGGACCCAGT TTATGGAAAA 
GGAAAACTTG GAGAAATCCA GGGCCTTATT CTGGGAATGT TGGATACCTT TAACTATGAA 
CAGACCCTGC TGGAAACGAC AACTAGCCTT CTAAACCAAG ATCTCCATTG GTCATTGTGT 
AACCTGAGAG CTTCAGTTAC CAGAGGACTG AATCCCAAGC AGGATTACTG CTCTATTTGT 
TTGCAGCAGT ACAAGAGACG CCAAGAAATG GCTGATGAAA TTATTGTCTT TAGCTGTGGC 
CATTTATATC ACTCGTTCTG TCTGCAAAGC AAAGAGTGCA CCATAGAAAT TGAAGGGCAA 
ACAAGATGGA CGTGCTACAA ATGCAGCTCA AGTAACAAAG CAGGAAAGTT GAGTGAAAAT 
TCATCTGAAG TTAAAAAGGG AAGGATAACC CCATCACAGG TAAAAATGTC CCCATCCTGT 
CATCTGTCCA AAGGGGATCC TGCTATTAAA AAGGCAACCT CAGAACCTGT TCTGGATCCA 
CAGCAAATCC AAGCATTTGA TCAGCTCTGC CATCTCTACC GAGGAAGTTC CAGGCTGGCC 
CTCCTCACGG AACTCTCCCA GAATCGCAGT GGCGAGAGCT GCAGGCCATT TGGAGGGTCC 
CAGAGTGGCC CCGCCTTCAA CAGCATCTTC CAGAAGGAGA ACTTCCAGCT GCAGCTCATT 
CCTCCCCCGG TGACTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015472153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327639.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa87754
Clone ID Related Accession (Same CDS sequence)	XM_015472153.1 , XM_015462058.1
Accession Version	XM_015472153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGA GAACTTGTGA ATCTCGATGC CTGTTTAGCG GTTCCAAGGG TGAAGTTTGC 
TGTATTGAAC CTCTCCACTC TAAGCCCGAG TTGAAAGACC ATCCCATCAC ACAATTTTCC 
TTATTGGCCA TGGCATCCTT AACAAAGATA CTGGTCATTG GATTGAAGCC ATCCTTGAAA 
GTGTGGATGA CTTTTCCTTA TGGACGGATG GATCCTTCCA GCGTTCCATT GCTGGCCTGG 
CACTTCGTGG CAGTGCATAA TTACGTGAAT CCCATGCTAG CCTTCTGCAG AGGAGATGTT 
GTTCATTTTC TCCTGGTTAA GAGAGACGAA TCTGGAGCGA TACATGTTAC TAAGCAAAAG 
CATCTTCACC TATACTATGA CCTCATCAAC TTTACTTGGA TAAACTCACG CACAATTGTG 
CTCTTGGACA GTGTAGAGAA GTTGCATGTG ATTGATCGGC AAACTCAGGA GGAACTGGAA 
ACAGTGGAGA TCTCAGAAGT TCAGTTGGTC TATAATAGCA GCCATTTCAA ATCACTAGCC 
ACTGGAGGAA ACGTGAGCCA GGCACTGGCT TTGGTTGGAG AGAAGGCTTG CTATCAATCC 
ATAAGTAGCT ATGGTGGTCA GATCTTTTAT TTGGGAACAA AATCTGTTTA TGTGATGATG 
CTGAGGAGCT GGAGGGAGAG AGTGGATCAC CTCTTGAAAC AAGACTGTCT TACAGAAGCT 
TTGGCTCTGG CATGGTCTTT CCATGAAGGA AAAGCAAAAG CAGTAGTAGG GTTATCAGGG 
GATGCCAGTA AGCGAAAGGC TATTGTTGCA GATCGGATGG TAGAAATCCT ATTGCATTAT 
GCAGACCGAG CTCTAAAAAA GTGCCCAGAC CAGGGAAAAA TCCAAGTGAT GGAGCAACAT 
TTTCAGGATA TGGTACCCGT CATAGTTGAT TACTGCCTTC TGCTACAGCG AAAGGATCTT 
TTATTTAGCC AGGTGTATGA CAAATTAAAT GAGAATTCAG TGGCCAAAGG AGTGTTTCTG 
GAGTGCCTTG AACCATATAT TTTAAGTGAT AAATTGGTGG GGATCACACC CCAGGTGATG 
AAAGACTTGA TTGTTCATTT CCAAGATAAA AAGTTAATGG AAAATGTGGA AGCCCTCATT 
GTGCATATGG ATATCACCAG CCTAGATATT CAGCAGATAG TTCTCATGTG TTGGGAAAAT 
CGACTATATG ATGCTATGAT CTATGTCTAC AACAGAGGCA TGAATGAATT TATTAGTCCA 
ATGGAGAAAC TTTTCAGAGT CATTGCACCT CCTTTGAATG CAGGAAAGAC GCTAACAGAT 
GAGCAAGTTG TTATGGGCAA CAAGCTTCTT GTATATATTA GCTGTTGTCT AGCAGGTCGT 
GCCTATCCGC TGGGTGACAT CCCTGAAGAT CTTGTTCCCT TGGTTAAAAA CCAGGTTTTT 
GAATTTCTAA TTCGCCTGCA TTCAACAGAA GCTCCCCCTG AAGAAGAAAT CTATCCTTAT 
ATTCGGACTT TGCTGCATTT CGACACACGA GAATTTCTAA ATGTATTGGC GCTGACTTTT 
GAAGATTTTA AAAATGATAA GCAAGCTGTA GAATATCAAC AACGTATTGT GGATATTTTG 
TTGAAGGTTA TGGTGGAGAA TTCAGATTTT ACGCCCTCAC AAGTGGGGTG TCTCTTTACC 
TTCCTTGCCC GGCAGCTTGC GAAGCCTGAC AACACCTTAT TTGTAAACAG AACTCTTTTT 
GATCAGGTCC TTGAATTCCT TTGTAGTCCT GATGATGACT CCCGACACTC TGAAAGACAG 
CAGGTCCTTT TAGAATTGCT GCAGGCTGGA GGCATAGTTC AGTTTGAAGA GAGTCGACTC 
ATCCGTATGG CAGAGAAAGC AGAATTCTAC CAAATTTGTG AATTTATGTA TGAACAAGAA 
CACCAATATG ACAGAATTAT TGATTGCTAC TTACGTGACC CACTACGAGA GGAAGAAGTC 
TTTAACTACA TTCACAATAT CTTATCCATT CCTGGATACA GTGTGGAGGA GAAGCAGTCT 
GTATGGCAGA AGGCTATGGA TCATATTGAG GAACTTGTGG CCCTGAAGCC CTGTAAAGCT 
GCAGAGCTGG TTGCTACTCA CTTTTCTGAA CAGATCGAAA CAGTCATTAA GAAACTTCAG 
AACCAGGTTT TGCTTTTCAA GTTTTTAAGA AGCCTTCTTG ACCCAAGGGA AGGTATTCAT 
GTAAATCAAG AATCGCTGCA GATATCTCCC TGTATAATAG AGCAGTTCAT TGAGCTCTTG 
TGTCAGTTCA GCCCAAACCA CGTTATTGAG ACGCTGCAAG TCCTTGAGTG CTACCGCCTA 
GAAGAAACTA TTCAGATTAC CCAGAAGTAT CAACTTTGTG AAGTCACTGC TTATCTATTA 
GAAAAGAAAG GAGATATTCA TGGTGCCTTC TTAATAATGC TAAAGAGACT ACAAAGCAAA 
CTTCAAGAGA TAATACGTCA AGATAAAAAT ACCAAAGAGG ATCCCTCATT GAAGGATGTT 
GAAGAGACCA TGGTAGAGAC GATTGCCCTC TGCCAGAGAA ATTCACATAA TTTGAACCAA 
CAGCAGAGAG AGGCACTCTG GTTTCCGTTA TTGGAGGCAA TGATGGCCCC TCAGAAGCTG 
TCTAGTTCAG CTTCACCTCA TCCACACTCT GAAGCTCTGA AATCTTTGAC CATGCAAGTT 
CTCAACAGTA TGGCAGCATT TATTGCCCTT CCATCCATCC TGCAGCGAAT CTTACAGGAC 
CCAGTTTATG GAAAAGGAAA ACTTGGAGAA ATCCAAGGCC TTATTCTGGG AATGTTGGAT 
ACCTTTAACT ATGAACAGAC CCTGCTGGAA ACGACAACTA GCCTTCTAAA CCAAGATCTC 
CATTGGTCAT TGTGTAACCT GAGAGCTTCA GTTACCAGAG GACTGAATCC CAAGCAGGAT 
TACTGCTCTA TTTGTTTGCA GCAGTACAAG AGACGCCAAG AAATGGCTGA TGAAATTATT 
GTCTTTAGCT GTGGCCATTT ATATCACTCG TTCTGTCTGC AAAGCAAAGA GTGCACCATA 
GAAATTGAAG GGCAAACAAG ATGGACGTGC TACATTTGCA TCTCAAGTAA CAAAGCAGGA 
AAGTTGAGTG AAAATTCATC TGAAGTTAAA AAGGGAAGGA TAACCCCATC ACAGGTAAAA 
ATGTCCCCAT CCTGTCATCT GTCCAAAGGG GATCCTGCTA TTAAAAAGGC AACCTCAGAA 
CCTGTTCTGG ATCCACAGCA AATCCAAGCA TTTGATCAGC TCTGCCATCT CTACCGAGGA 
AGTTCCAGGC TGGCCCTCCT CACGGAACTC TCCCAGAATC GCAGTGGCGA GAGCTGCAGG 
CCATTTGGAG GGTCCCAGAG TGGCCCCGCC TTCAACAGCA TCTTCCAGAA GGAGAACTTC 
CAGCTGCAGC TCATTCCTCC CCCGGTGACT GAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327639.1
CDS	94..3552
Translation	MGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015472153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472153.1

ORF Insert Sequence:

ATGGGAGTGA GAACTTGTGA ATCTCGATGC CTGTTTAGCG GTTCCAAGGG TGAAGTTTGC 
TGTATTGAAC CTCTCCACTC TAAGCCCGAG TTGAAAGACC ATCCCATCAC ACAATTTTCC 
TTATTGGCCA TGGCATCCTT AACAAAGATA CTGGTCATTG GATTGAAGCC ATCCTTGAAA 
GTGTGGATGA CTTTTCCTTA TGGACGGATG GATCCTTCCA GCGTTCCATT GCTGGCCTGG 
CACTTCGTGG CAGTGCATAA TTACGTGAAT CCCATGCTAG CCTTCTGCAG AGGAGATGTT 
GTTCATTTTC TCCTGGTTAA GAGAGACGAA TCTGGAGCGA TACATGTTAC TAAGCAAAAG 
CATCTTCACC TATACTATGA CCTCATCAAC TTTACTTGGA TAAACTCACG CACAATTGTG 
CTCTTGGACA GTGTAGAGAA GTTGCATGTG ATTGATCGGC AAACTCAGGA GGAACTGGAA 
ACAGTGGAGA TCTCAGAAGT TCAGTTGGTC TATAATAGCA GCCATTTCAA ATCACTAGCC 
ACTGGAGGAA ACGTGAGCCA GGCACTGGCT TTGGTTGGAG AGAAGGCTTG CTATCAATCC 
ATAAGTAGCT ATGGTGGTCA GATCTTTTAT TTGGGAACAA AATCTGTTTA TGTGATGATG 
CTGAGGAGCT GGAGGGAGAG AGTGGATCAC CTCTTGAAAC AAGACTGTCT TACAGAAGCT 
TTGGCTCTGG CATGGTCTTT CCATGAAGGA AAAGCAAAAG CAGTAGTAGG GTTATCAGGG 
GATGCCAGTA AGCGAAAGGC TATTGTTGCA GATCGGATGG TAGAAATCCT ATTGCATTAT 
GCAGACCGAG CTCTAAAAAA GTGCCCAGAC CAGGGAAAAA TCCAAGTGAT GGAGCAACAT 
TTTCAGGATA TGGTACCCGT CATAGTTGAT TACTGCCTTC TGCTACAGCG AAAGGATCTT 
TTATTTAGCC AGGTGTATGA CAAATTAAAT GAGAATTCAG TGGCCAAAGG AGTGTTTCTG 
GAGTGCCTTG AACCATATAT TTTAAGTGAT AAATTGGTGG GGATCACACC CCAGGTGATG 
AAAGACTTGA TTGTTCATTT CCAAGATAAA AAGTTAATGG AAAATGTGGA AGCCCTCATT 
GTGCATATGG ATATCACCAG CCTAGATATT CAGCAGATAG TTCTCATGTG TTGGGAAAAT 
CGACTATATG ATGCTATGAT CTATGTCTAC AACAGAGGCA TGAATGAATT TATTAGTCCA 
ATGGAGAAAC TTTTCAGAGT CATTGCACCT CCTTTGAATG CAGGAAAGAC GCTAACAGAT 
GAGCAAGTTG TTATGGGCAA CAAGCTTCTT GTATATATTA GCTGTTGTCT AGCAGGTCGT 
GCCTATCCGC TGGGTGACAT CCCTGAAGAT CTTGTTCCCT TGGTTAAAAA CCAGGTTTTT 
GAATTTCTAA TTCGCCTGCA TTCAACAGAA GCTCCCCCTG AAGAAGAAAT CTATCCTTAT 
ATTCGGACTT TGCTGCATTT CGACACACGA GAATTTCTAA ATGTATTGGC GCTGACTTTT 
GAAGATTTTA AAAATGATAA GCAAGCTGTA GAATATCAAC AACGTATTGT GGATATTTTG 
TTGAAGGTTA TGGTGGAGAA TTCAGATTTT ACGCCCTCAC AAGTGGGGTG TCTCTTTACC 
TTCCTTGCCC GGCAGCTTGC GAAGCCTGAC AACACCTTAT TTGTAAACAG AACTCTTTTT 
GATCAGGTCC TTGAATTCCT TTGTAGTCCT GATGATGACT CCCGACACTC TGAAAGACAG 
CAGGTCCTTT TAGAATTGCT GCAGGCTGGA GGCATAGTTC AGTTTGAAGA GAGTCGACTC 
ATCCGTATGG CAGAGAAAGC AGAATTCTAC CAAATTTGTG AATTTATGTA TGAACAAGAA 
CACCAATATG ACAGAATTAT TGATTGCTAC TTACGTGACC CACTACGAGA GGAAGAAGTC 
TTTAACTACA TTCACAATAT CTTATCCATT CCTGGATACA GTGTGGAGGA GAAGCAGTCT 
GTATGGCAGA AGGCTATGGA TCATATTGAG GAACTTGTGG CCCTGAAGCC CTGTAAAGCT 
GCAGAGCTGG TTGCTACTCA CTTTTCTGAA CAGATCGAAA CAGTCATTAA GAAACTTCAG 
AACCAGGTTT TGCTTTTCAA GTTTTTAAGA AGCCTTCTTG ACCCAAGGGA AGGTATTCAT 
GTAAATCAAG AATCGCTGCA GATATCTCCC TGTATAATAG AGCAGTTCAT TGAGCTCTTG 
TGTCAGTTCA GCCCAAACCA CGTTATTGAG ACGCTGCAAG TCCTTGAGTG CTACCGCCTA 
GAAGAAACTA TTCAGATTAC CCAGAAGTAT CAACTTTGTG AAGTCACTGC TTATCTATTA 
GAAAAGAAAG GAGATATTCA TGGTGCCTTC TTAATAATGC TAAAGAGACT ACAAAGCAAA 
CTTCAAGAGA TAATACGTCA AGATAAAAAT ACCAAAGAGG ATCCCTCATT GAAGGATGTT 
GAAGAGACCA TGGTAGAGAC GATTGCCCTC TGCCAGAGAA ATTCACATAA TTTGAACCAA 
CAGCAGAGAG AGGCACTCTG GTTTCCGTTA TTGGAGGCAA TGATGGCCCC TCAGAAGCTG 
TCTAGTTCAG CTTCACCTCA TCCACACTCT GAAGCTCTGA AATCTTTGAC CATGCAAGTT 
CTCAACAGTA TGGCAGCATT TATTGCCCTT CCATCCATCC TGCAGCGAAT CTTACAGGAC 
CCAGTTTATG GAAAAGGAAA ACTTGGAGAA ATCCAAGGCC TTATTCTGGG AATGTTGGAT 
ACCTTTAACT ATGAACAGAC CCTGCTGGAA ACGACAACTA GCCTTCTAAA CCAAGATCTC 
CATTGGTCAT TGTGTAACCT GAGAGCTTCA GTTACCAGAG GACTGAATCC CAAGCAGGAT 
TACTGCTCTA TTTGTTTGCA GCAGTACAAG AGACGCCAAG AAATGGCTGA TGAAATTATT 
GTCTTTAGCT GTGGCCATTT ATATCACTCG TTCTGTCTGC AAAGCAAAGA GTGCACCATA 
GAAATTGAAG GGCAAACAAG ATGGACGTGC TACATTTGCA TCTCAAGTAA CAAAGCAGGA 
AAGTTGAGTG AAAATTCATC TGAAGTTAAA AAGGGAAGGA TAACCCCATC ACAGGTAAAA 
ATGTCCCCAT CCTGTCATCT GTCCAAAGGG GATCCTGCTA TTAAAAAGGC AACCTCAGAA 
CCTGTTCTGG ATCCACAGCA AATCCAAGCA TTTGATCAGC TCTGCCATCT CTACCGAGGA 
AGTTCCAGGC TGGCCCTCCT CACGGAACTC TCCCAGAATC GCAGTGGCGA GAGCTGCAGG 
CCATTTGGAG GGTCCCAGAG TGGCCCCGCC TTCAACAGCA TCTTCCAGAA GGAGAACTTC 
CAGCTGCAGC TCATTCCTCC CCCGGTGACT GAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015462058.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317544.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.

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CloneID	OBa87754
Clone ID Related Accession (Same CDS sequence)	XM_015472153.1 , XM_015462058.1
Accession Version	XM_015462058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644522.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGA GAACTTGTGA ATCTCGATGC CTGTTTAGCG GTTCCAAGGG TGAAGTTTGC 
TGTATTGAAC CTCTCCACTC TAAGCCCGAG TTGAAAGACC ATCCCATCAC ACAATTTTCC 
TTATTGGCCA TGGCATCCTT AACAAAGATA CTGGTCATTG GATTGAAGCC ATCCTTGAAA 
GTGTGGATGA CTTTTCCTTA TGGACGGATG GATCCTTCCA GCGTTCCATT GCTGGCCTGG 
CACTTCGTGG CAGTGCATAA TTACGTGAAT CCCATGCTAG CCTTCTGCAG AGGAGATGTT 
GTTCATTTTC TCCTGGTTAA GAGAGACGAA TCTGGAGCGA TACATGTTAC TAAGCAAAAG 
CATCTTCACC TATACTATGA CCTCATCAAC TTTACTTGGA TAAACTCACG CACAATTGTG 
CTCTTGGACA GTGTAGAGAA GTTGCATGTG ATTGATCGGC AAACTCAGGA GGAACTGGAA 
ACAGTGGAGA TCTCAGAAGT TCAGTTGGTC TATAATAGCA GCCATTTCAA ATCACTAGCC 
ACTGGAGGAA ACGTGAGCCA GGCACTGGCT TTGGTTGGAG AGAAGGCTTG CTATCAATCC 
ATAAGTAGCT ATGGTGGTCA GATCTTTTAT TTGGGAACAA AATCTGTTTA TGTGATGATG 
CTGAGGAGCT GGAGGGAGAG AGTGGATCAC CTCTTGAAAC AAGACTGTCT TACAGAAGCT 
TTGGCTCTGG CATGGTCTTT CCATGAAGGA AAAGCAAAAG CAGTAGTAGG GTTATCAGGG 
GATGCCAGTA AGCGAAAGGC TATTGTTGCA GATCGGATGG TAGAAATCCT ATTGCATTAT 
GCAGACCGAG CTCTAAAAAA GTGCCCAGAC CAGGGAAAAA TCCAAGTGAT GGAGCAACAT 
TTTCAGGATA TGGTACCCGT CATAGTTGAT TACTGCCTTC TGCTACAGCG AAAGGATCTT 
TTATTTAGCC AGGTGTATGA CAAATTAAAT GAGAATTCAG TGGCCAAAGG AGTGTTTCTG 
GAGTGCCTTG AACCATATAT TTTAAGTGAT AAATTGGTGG GGATCACACC CCAGGTGATG 
AAAGACTTGA TTGTTCATTT CCAAGATAAA AAGTTAATGG AAAATGTGGA AGCCCTCATT 
GTGCATATGG ATATCACCAG CCTAGATATT CAGCAGATAG TTCTCATGTG TTGGGAAAAT 
CGACTATATG ATGCTATGAT CTATGTCTAC AACAGAGGCA TGAATGAATT TATTAGTCCA 
ATGGAGAAAC TTTTCAGAGT CATTGCACCT CCTTTGAATG CAGGAAAGAC GCTAACAGAT 
GAGCAAGTTG TTATGGGCAA CAAGCTTCTT GTATATATTA GCTGTTGTCT AGCAGGTCGT 
GCCTATCCGC TGGGTGACAT CCCTGAAGAT CTTGTTCCCT TGGTTAAAAA CCAGGTTTTT 
GAATTTCTAA TTCGCCTGCA TTCAACAGAA GCTCCCCCTG AAGAAGAAAT CTATCCTTAT 
ATTCGGACTT TGCTGCATTT CGACACACGA GAATTTCTAA ATGTATTGGC GCTGACTTTT 
GAAGATTTTA AAAATGATAA GCAAGCTGTA GAATATCAAC AACGTATTGT GGATATTTTG 
TTGAAGGTTA TGGTGGAGAA TTCAGATTTT ACGCCCTCAC AAGTGGGGTG TCTCTTTACC 
TTCCTTGCCC GGCAGCTTGC GAAGCCTGAC AACACCTTAT TTGTAAACAG AACTCTTTTT 
GATCAGGTCC TTGAATTCCT TTGTAGTCCT GATGATGACT CCCGACACTC TGAAAGACAG 
CAGGTCCTTT TAGAATTGCT GCAGGCTGGA GGCATAGTTC AGTTTGAAGA GAGTCGACTC 
ATCCGTATGG CAGAGAAAGC AGAATTCTAC CAAATTTGTG AATTTATGTA TGAACAAGAA 
CACCAATATG ACAGAATTAT TGATTGCTAC TTACGTGACC CACTACGAGA GGAAGAAGTC 
TTTAACTACA TTCACAATAT CTTATCCATT CCTGGATACA GTGTGGAGGA GAAGCAGTCT 
GTATGGCAGA AGGCTATGGA TCATATTGAG GAACTTGTGG CCCTGAAGCC CTGTAAAGCT 
GCAGAGCTGG TTGCTACTCA CTTTTCTGAA CAGATCGAAA CAGTCATTAA GAAACTTCAG 
AACCAGGTTT TGCTTTTCAA GTTTTTAAGA AGCCTTCTTG ACCCAAGGGA AGGTATTCAT 
GTAAATCAAG AATCGCTGCA GATATCTCCC TGTATAATAG AGCAGTTCAT TGAGCTCTTG 
TGTCAGTTCA GCCCAAACCA CGTTATTGAG ACGCTGCAAG TCCTTGAGTG CTACCGCCTA 
GAAGAAACTA TTCAGATTAC CCAGAAGTAT CAACTTTGTG AAGTCACTGC TTATCTATTA 
GAAAAGAAAG GAGATATTCA TGGTGCCTTC TTAATAATGC TAAAGAGACT ACAAAGCAAA 
CTTCAAGAGA TAATACGTCA AGATAAAAAT ACCAAAGAGG ATCCCTCATT GAAGGATGTT 
GAAGAGACCA TGGTAGAGAC GATTGCCCTC TGCCAGAGAA ATTCACATAA TTTGAACCAA 
CAGCAGAGAG AGGCACTCTG GTTTCCGTTA TTGGAGGCAA TGATGGCCCC TCAGAAGCTG 
TCTAGTTCAG CTTCACCTCA TCCACACTCT GAAGCTCTGA AATCTTTGAC CATGCAAGTT 
CTCAACAGTA TGGCAGCATT TATTGCCCTT CCATCCATCC TGCAGCGAAT CTTACAGGAC 
CCAGTTTATG GAAAAGGAAA ACTTGGAGAA ATCCAAGGCC TTATTCTGGG AATGTTGGAT 
ACCTTTAACT ATGAACAGAC CCTGCTGGAA ACGACAACTA GCCTTCTAAA CCAAGATCTC 
CATTGGTCAT TGTGTAACCT GAGAGCTTCA GTTACCAGAG GACTGAATCC CAAGCAGGAT 
TACTGCTCTA TTTGTTTGCA GCAGTACAAG AGACGCCAAG AAATGGCTGA TGAAATTATT 
GTCTTTAGCT GTGGCCATTT ATATCACTCG TTCTGTCTGC AAAGCAAAGA GTGCACCATA 
GAAATTGAAG GGCAAACAAG ATGGACGTGC TACATTTGCA TCTCAAGTAA CAAAGCAGGA 
AAGTTGAGTG AAAATTCATC TGAAGTTAAA AAGGGAAGGA TAACCCCATC ACAGGTAAAA 
ATGTCCCCAT CCTGTCATCT GTCCAAAGGG GATCCTGCTA TTAAAAAGGC AACCTCAGAA 
CCTGTTCTGG ATCCACAGCA AATCCAAGCA TTTGATCAGC TCTGCCATCT CTACCGAGGA 
AGTTCCAGGC TGGCCCTCCT CACGGAACTC TCCCAGAATC GCAGTGGCGA GAGCTGCAGG 
CCATTTGGAG GGTCCCAGAG TGGCCCCGCC TTCAACAGCA TCTTCCAGAA GGAGAACTTC 
CAGCTGCAGC TCATTCCTCC CCCGGTGACT GAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317544.1
CDS	94..3552
Translation	MGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015462058.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462058.1

ORF Insert Sequence:

ATGGGAGTGA GAACTTGTGA ATCTCGATGC CTGTTTAGCG GTTCCAAGGG TGAAGTTTGC 
TGTATTGAAC CTCTCCACTC TAAGCCCGAG TTGAAAGACC ATCCCATCAC ACAATTTTCC 
TTATTGGCCA TGGCATCCTT AACAAAGATA CTGGTCATTG GATTGAAGCC ATCCTTGAAA 
GTGTGGATGA CTTTTCCTTA TGGACGGATG GATCCTTCCA GCGTTCCATT GCTGGCCTGG 
CACTTCGTGG CAGTGCATAA TTACGTGAAT CCCATGCTAG CCTTCTGCAG AGGAGATGTT 
GTTCATTTTC TCCTGGTTAA GAGAGACGAA TCTGGAGCGA TACATGTTAC TAAGCAAAAG 
CATCTTCACC TATACTATGA CCTCATCAAC TTTACTTGGA TAAACTCACG CACAATTGTG 
CTCTTGGACA GTGTAGAGAA GTTGCATGTG ATTGATCGGC AAACTCAGGA GGAACTGGAA 
ACAGTGGAGA TCTCAGAAGT TCAGTTGGTC TATAATAGCA GCCATTTCAA ATCACTAGCC 
ACTGGAGGAA ACGTGAGCCA GGCACTGGCT TTGGTTGGAG AGAAGGCTTG CTATCAATCC 
ATAAGTAGCT ATGGTGGTCA GATCTTTTAT TTGGGAACAA AATCTGTTTA TGTGATGATG 
CTGAGGAGCT GGAGGGAGAG AGTGGATCAC CTCTTGAAAC AAGACTGTCT TACAGAAGCT 
TTGGCTCTGG CATGGTCTTT CCATGAAGGA AAAGCAAAAG CAGTAGTAGG GTTATCAGGG 
GATGCCAGTA AGCGAAAGGC TATTGTTGCA GATCGGATGG TAGAAATCCT ATTGCATTAT 
GCAGACCGAG CTCTAAAAAA GTGCCCAGAC CAGGGAAAAA TCCAAGTGAT GGAGCAACAT 
TTTCAGGATA TGGTACCCGT CATAGTTGAT TACTGCCTTC TGCTACAGCG AAAGGATCTT 
TTATTTAGCC AGGTGTATGA CAAATTAAAT GAGAATTCAG TGGCCAAAGG AGTGTTTCTG 
GAGTGCCTTG AACCATATAT TTTAAGTGAT AAATTGGTGG GGATCACACC CCAGGTGATG 
AAAGACTTGA TTGTTCATTT CCAAGATAAA AAGTTAATGG AAAATGTGGA AGCCCTCATT 
GTGCATATGG ATATCACCAG CCTAGATATT CAGCAGATAG TTCTCATGTG TTGGGAAAAT 
CGACTATATG ATGCTATGAT CTATGTCTAC AACAGAGGCA TGAATGAATT TATTAGTCCA 
ATGGAGAAAC TTTTCAGAGT CATTGCACCT CCTTTGAATG CAGGAAAGAC GCTAACAGAT 
GAGCAAGTTG TTATGGGCAA CAAGCTTCTT GTATATATTA GCTGTTGTCT AGCAGGTCGT 
GCCTATCCGC TGGGTGACAT CCCTGAAGAT CTTGTTCCCT TGGTTAAAAA CCAGGTTTTT 
GAATTTCTAA TTCGCCTGCA TTCAACAGAA GCTCCCCCTG AAGAAGAAAT CTATCCTTAT 
ATTCGGACTT TGCTGCATTT CGACACACGA GAATTTCTAA ATGTATTGGC GCTGACTTTT 
GAAGATTTTA AAAATGATAA GCAAGCTGTA GAATATCAAC AACGTATTGT GGATATTTTG 
TTGAAGGTTA TGGTGGAGAA TTCAGATTTT ACGCCCTCAC AAGTGGGGTG TCTCTTTACC 
TTCCTTGCCC GGCAGCTTGC GAAGCCTGAC AACACCTTAT TTGTAAACAG AACTCTTTTT 
GATCAGGTCC TTGAATTCCT TTGTAGTCCT GATGATGACT CCCGACACTC TGAAAGACAG 
CAGGTCCTTT TAGAATTGCT GCAGGCTGGA GGCATAGTTC AGTTTGAAGA GAGTCGACTC 
ATCCGTATGG CAGAGAAAGC AGAATTCTAC CAAATTTGTG AATTTATGTA TGAACAAGAA 
CACCAATATG ACAGAATTAT TGATTGCTAC TTACGTGACC CACTACGAGA GGAAGAAGTC 
TTTAACTACA TTCACAATAT CTTATCCATT CCTGGATACA GTGTGGAGGA GAAGCAGTCT 
GTATGGCAGA AGGCTATGGA TCATATTGAG GAACTTGTGG CCCTGAAGCC CTGTAAAGCT 
GCAGAGCTGG TTGCTACTCA CTTTTCTGAA CAGATCGAAA CAGTCATTAA GAAACTTCAG 
AACCAGGTTT TGCTTTTCAA GTTTTTAAGA AGCCTTCTTG ACCCAAGGGA AGGTATTCAT 
GTAAATCAAG AATCGCTGCA GATATCTCCC TGTATAATAG AGCAGTTCAT TGAGCTCTTG 
TGTCAGTTCA GCCCAAACCA CGTTATTGAG ACGCTGCAAG TCCTTGAGTG CTACCGCCTA 
GAAGAAACTA TTCAGATTAC CCAGAAGTAT CAACTTTGTG AAGTCACTGC TTATCTATTA 
GAAAAGAAAG GAGATATTCA TGGTGCCTTC TTAATAATGC TAAAGAGACT ACAAAGCAAA 
CTTCAAGAGA TAATACGTCA AGATAAAAAT ACCAAAGAGG ATCCCTCATT GAAGGATGTT 
GAAGAGACCA TGGTAGAGAC GATTGCCCTC TGCCAGAGAA ATTCACATAA TTTGAACCAA 
CAGCAGAGAG AGGCACTCTG GTTTCCGTTA TTGGAGGCAA TGATGGCCCC TCAGAAGCTG 
TCTAGTTCAG CTTCACCTCA TCCACACTCT GAAGCTCTGA AATCTTTGAC CATGCAAGTT 
CTCAACAGTA TGGCAGCATT TATTGCCCTT CCATCCATCC TGCAGCGAAT CTTACAGGAC 
CCAGTTTATG GAAAAGGAAA ACTTGGAGAA ATCCAAGGCC TTATTCTGGG AATGTTGGAT 
ACCTTTAACT ATGAACAGAC CCTGCTGGAA ACGACAACTA GCCTTCTAAA CCAAGATCTC 
CATTGGTCAT TGTGTAACCT GAGAGCTTCA GTTACCAGAG GACTGAATCC CAAGCAGGAT 
TACTGCTCTA TTTGTTTGCA GCAGTACAAG AGACGCCAAG AAATGGCTGA TGAAATTATT 
GTCTTTAGCT GTGGCCATTT ATATCACTCG TTCTGTCTGC AAAGCAAAGA GTGCACCATA 
GAAATTGAAG GGCAAACAAG ATGGACGTGC TACATTTGCA TCTCAAGTAA CAAAGCAGGA 
AAGTTGAGTG AAAATTCATC TGAAGTTAAA AAGGGAAGGA TAACCCCATC ACAGGTAAAA 
ATGTCCCCAT CCTGTCATCT GTCCAAAGGG GATCCTGCTA TTAAAAAGGC AACCTCAGAA 
CCTGTTCTGG ATCCACAGCA AATCCAAGCA TTTGATCAGC TCTGCCATCT CTACCGAGGA 
AGTTCCAGGC TGGCCCTCCT CACGGAACTC TCCCAGAATC GCAGTGGCGA GAGCTGCAGG 
CCATTTGGAG GGTCCCAGAG TGGCCCCGCC TTCAACAGCA TCTTCCAGAA GGAGAACTTC 
CAGCTGCAGC TCATTCCTCC CCCGGTGACT GAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015472149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327635.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa15784
Clone ID Related Accession (Same CDS sequence)	XM_015472149.1 , XM_015462056.1 , XM_001251842.7 , XM_002684849.5
Accession Version	XM_015472149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327635.1
CDS	1..4287
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015472149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472149.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015462056.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317542.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa15784
Clone ID Related Accession (Same CDS sequence)	XM_015472149.1 , XM_015462056.1 , XM_001251842.7 , XM_002684849.5
Accession Version	XM_015462056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644522.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317542.1
CDS	1..4287
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015462056.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462056.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_001251842.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001251843.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

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CloneID	OBa15784
Clone ID Related Accession (Same CDS sequence)	XM_015472149.1 , XM_015462056.1 , XM_001251842.7 , XM_002684849.5
Accession Version	XM_001251842.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644522.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001251842.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001251843.1
CDS	212..4498
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_001251842.7
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001251842.7

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_002684849.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684895.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

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CloneID	OBa15784
Clone ID Related Accession (Same CDS sequence)	XM_015472149.1 , XM_015462056.1 , XM_001251842.7 , XM_002684849.5
Accession Version	XM_002684849.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684849.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684895.1
CDS	212..4498
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_002684849.5
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684849.5

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGTAAAAAT GTCCCCATCC 
TGTCATCTGT CCAAAGGGGA TCCTGCTATT AAAAAGGCAA CCTCAGAACC TGTTCTGGAT 
CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCATCTCT ACCGAGGAAG TTCCAGGCTG 
GCCCTCCTCA CGGAACTCTC CCAGAATCGC AGTGGCGAGA GCTGCAGGCC ATTTGGAGGG 
TCCCAGAGTG GCCCCGCCTT CAACAGCATC TTCCAGAAGG AGAACTTCCA GCTGCAGCTC 
ATTCCTCCCC CGGTGACTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015472150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327636.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa15785
Clone ID Related Accession (Same CDS sequence)	XM_015472150.1 , XM_015462057.1
Accession Version	XM_015472150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAAGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACATTTG CATCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327636.1
CDS	1..4281
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015472150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472150.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAAGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACATTTG CATCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_015462057.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317543.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa15785
Clone ID Related Accession (Same CDS sequence)	XM_015472150.1 , XM_015462057.1
Accession Version	XM_015462057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644522.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAAGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACATTTG CATCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317543.1
CDS	1..4281
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQVKMSPSCHLSKGDPAIKKATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_015462057.1
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462057.1

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGATCAG 
AATCAAGCTT TGCGACTCTG CTTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCTATC 
TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC TAAGGGACAG 
ATCACTATGT GGGATTTGGC CAGTGGAAAG CTTCTAAGAT CAATAACAGA TGCTCATCCT 
CCGGGAACAG CAATATTACA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT 
GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACTTGT 
GAATCTCGAT GCCTGTTTAG CGGTTCCAAG GGTGAAGTTT GCTGTATTGA ACCTCTCCAC 
TCTAAGCCCG AGTTGAAAGA CCATCCCATC ACACAATTTT CCTTATTGGC CATGGCATCC 
TTAACAAAGA TACTGGTCAT TGGATTGAAG CCATCCTTGA AAGTGTGGAT GACTTTTCCT 
TATGGACGGA TGGATCCTTC CAGCGTTCCA TTGCTGGCCT GGCACTTCGT GGCAGTGCAT 
AATTACGTGA ATCCCATGCT AGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTCCTGGTT 
AAGAGAGACG AATCTGGAGC GATACATGTT ACTAAGCAAA AGCATCTTCA CCTATACTAT 
GACCTCATCA ACTTTACTTG GATAAACTCA CGCACAATTG TGCTCTTGGA CAGTGTAGAG 
AAGTTGCATG TGATTGATCG GCAAACTCAG GAGGAACTGG AAACAGTGGA GATCTCAGAA 
GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCACTAG CCACTGGAGG AAACGTGAGC 
CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGCTATCAAT CCATAAGTAG CTATGGTGGT 
CAGATCTTTT ATTTGGGAAC AAAATCTGTT TATGTGATGA TGCTGAGGAG CTGGAGGGAG 
AGAGTGGATC ACCTCTTGAA ACAAGACTGT CTTACAGAAG CTTTGGCTCT GGCATGGTCT 
TTCCATGAAG GAAAAGCAAA AGCAGTAGTA GGGTTATCAG GGGATGCCAG TAAGCGAAAG 
GCTATTGTTG CAGATCGGAT GGTAGAAATC CTATTGCATT ATGCAGACCG AGCTCTAAAA 
AAGTGCCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAAC ATTTTCAGGA TATGGTACCC 
GTCATAGTTG ATTACTGCCT TCTGCTACAG CGAAAGGATC TTTTATTTAG CCAGGTGTAT 
GACAAATTAA ATGAGAATTC AGTGGCCAAA GGAGTGTTTC TGGAGTGCCT TGAACCATAT 
ATTTTAAGTG ATAAATTGGT GGGGATCACA CCCCAGGTGA TGAAAGACTT GATTGTTCAT 
TTCCAAGATA AAAAGTTAAT GGAAAATGTG GAAGCCCTCA TTGTGCATAT GGATATCACC 
AGCCTAGATA TTCAGCAGAT AGTTCTCATG TGTTGGGAAA ATCGACTATA TGATGCTATG 
ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA 
GTCATTGCAC CTCCTTTGAA TGCAGGAAAG ACGCTAACAG ATGAGCAAGT TGTTATGGGC 
AACAAGCTTC TTGTATATAT TAGCTGTTGT CTAGCAGGTC GTGCCTATCC GCTGGGTGAC 
ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT AATTCGCCTG 
CATTCAACAG AAGCTCCCCC TGAAGAAGAA ATCTATCCTT ATATTCGGAC TTTGCTGCAT 
TTCGACACAC GAGAATTTCT AAATGTATTG GCGCTGACTT TTGAAGATTT TAAAAATGAT 
AAGCAAGCTG TAGAATATCA ACAACGTATT GTGGATATTT TGTTGAAGGT TATGGTGGAG 
AATTCAGATT TTACGCCCTC ACAAGTGGGG TGTCTCTTTA CCTTCCTTGC CCGGCAGCTT 
GCGAAGCCTG ACAACACCTT ATTTGTAAAC AGAACTCTTT TTGATCAGGT CCTTGAATTC 
CTTTGTAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAATTG 
CTGCAGGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGAC TCATCCGTAT GGCAGAGAAA 
GCAGAATTCT ACCAAATTTG TGAATTTATG TATGAACAAG AACACCAATA TGACAGAATT 
ATTGATTGCT ACTTACGTGA CCCACTACGA GAGGAAGAAG TCTTTAACTA CATTCACAAT 
ATCTTATCCA TTCCTGGATA CAGTGTGGAG GAGAAGCAGT CTGTATGGCA GAAGGCTATG 
GATCATATTG AGGAACTTGT GGCCCTGAAG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT 
CACTTTTCTG AACAGATCGA AACAGTCATT AAGAAACTTC AGAACCAGGT TTTGCTTTTC 
AAGTTTTTAA GAAGCCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATCGCTG 
CAGATATCTC CCTGTATAAT AGAGCAGTTC ATTGAGCTCT TGTGTCAGTT CAGCCCAAAC 
CACGTTATTG AGACGCTGCA AGTCCTTGAG TGCTACCGCC TAGAAGAAAC TATTCAGATT 
ACCCAGAAGT ATCAACTTTG TGAAGTCACT GCTTATCTAT TAGAAAAGAA AGGAGATATT 
CATGGTGCCT TCTTAATAAT GCTAAAGAGA CTACAAAGCA AACTTCAAGA GATAATACGT 
CAAGATAAAA ATACCAAAGA GGATCCCTCA TTGAAGGATG TTGAAGAGAC CATGGTAGAG 
ACGATTGCCC TCTGCCAGAG AAATTCACAT AATTTGAACC AACAGCAGAG AGAGGCACTC 
TGGTTTCCGT TATTGGAGGC AATGATGGCC CCTCAGAAGC TGTCTAGTTC AGCTTCACCT 
CATCCACACT CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTCAACAG TATGGCAGCA 
TTTATTGCCC TTCCATCCAT CCTGCAGCGA ATCTTACAGG ACCCAGTTTA TGGAAAAGGA 
AAACTTGGAG AAATCCAAGG CCTTATTCTG GGAATGTTGG ATACCTTTAA CTATGAACAG 
ACCCTGCTGG AAACGACAAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC 
CTGAGAGCTT CAGTTACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATTTGTTTG 
CAGCAGTACA AGAGACGCCA AGAAATGGCT GATGAAATTA TTGTCTTTAG CTGTGGCCAT 
TTATATCACT CGTTCTGTCT GCAAAGCAAA GAGTGCACCA TAGAAATTGA AGGGCAAACA 
AGATGGACGT GCTACATTTG CATCTCAAGT AACAAAGCAG GAAAGTTGAG TGAAAATTCA 
TCTGAAGTTA AAAAGGGAAG GATAACCCCA TCACAGGTAA AAATGTCCCC ATCCTGTCAT 
CTGTCCAAAG GGGATCCTGC TATTAAAAAG GCAACCTCAG AACCTGTTCT GGATCCACAG 
CAAATCCAAG CATTTGATCA GCTCTGCCAT CTCTACCGAG GAAGTTCCAG GCTGGCCCTC 
CTCACGGAAC TCTCCCAGAA TCGCAGTGGC GAGAGCTGCA GGCCATTTGG AGGGTCCCAG 
AGTGGCCCCG CCTTCAACAG CATCTTCCAG AAGGAGAACT TCCAGCTGCA GCTCATTCCT 
CCCCCGGTGA CTGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_005201567.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201624.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

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CloneID	OBa15786
Clone ID Related Accession (Same CDS sequence)	XM_005201567.3 , XM_005192219.3
Accession Version	XM_005201567.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201567.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGCAACCTC AGAACCTGTT 
CTGGATCCAC AGCAAATCCA AGCATTTGAT CAGCTCTGCC ATCTCTACCG AGGAAGTTCC 
AGGCTGGCCC TCCTCACGGA ACTCTCCCAG AATCGCAGTG GCGAGAGCTG CAGGCCATTT 
GGAGGGTCCC AGAGTGGCCC CGCCTTCAAC AGCATCTTCC AGAAGGAGAA CTTCCAGCTG 
CAGCTCATTC CTCCCCCGGT GACTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201624.1
CDS	212..4444
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_005201567.3
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201567.3

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGCAACCTC AGAACCTGTT 
CTGGATCCAC AGCAAATCCA AGCATTTGAT CAGCTCTGCC ATCTCTACCG AGGAAGTTCC 
AGGCTGGCCC TCCTCACGGA ACTCTCCCAG AATCGCAGTG GCGAGAGCTG CAGGCCATTT 
GGAGGGTCCC AGAGTGGCCC CGCCTTCAAC AGCATCTTCC AGAAGGAGAA CTTCCAGCTG 
CAGCTCATTC CTCCCCCGGT GACTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_005192219.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192276.1 Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

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CloneID	OBa15786
Clone ID Related Accession (Same CDS sequence)	XM_005201567.3 , XM_005192219.3
Accession Version	XM_005192219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644522.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGCAACCTC AGAACCTGTT 
CTGGATCCAC AGCAAATCCA AGCATTTGAT CAGCTCTGCC ATCTCTACCG AGGAAGTTCC 
AGGCTGGCCC TCCTCACGGA ACTCTCCCAG AATCGCAGTG GCGAGAGCTG CAGGCCATTT 
GGAGGGTCCC AGAGTGGCCC CGCCTTCAAC AGCATCTTCC AGAAGGAGAA CTTCCAGCTG 
CAGCTCATTC CTCCCCCGGT GACTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192276.1
CDS	212..4444
Translation	MENEPDHENVEQSLCAKTTDEELNKSFSLEASLSKFSYIDLDKELEFRNDLIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTIDSHSYDTSSVASSDSGDRTNLKRKKKLPDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKPELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAVHNYVNPMLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRQTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLRSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILLHYADRALKKCPDQGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLFSQVYDKLNENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQIVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSTEAPPEEEIYPYIRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYEQEHQYDRIIDCYLRDPLREEEVFNYIHNILSIPGYSVEEKQSVWQKAMDHIEELVALKPCKAAELVATHFSEQIETVIKKLQNQVLLFKFLRSLLDPREGIHVNQESLQISPCIIEQFIELLCQFSPNHVIETLQVLECYRLEETIQITQKYQLCEVTAYLLEKKGDIHGAFLIMLKRLQSKLQEIIRQDKNTKEDPSLKDVEETMVETIALCQRNSHNLNQQQREALWFPLLEAMMAPQKLSSSASPHPHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYHSFCLQSKECTIEIEGQTRWTCYICISSNKAGKLSENSSEVKKGRITPSQATSEPVLDPQQIQAFDQLCHLYRGSSRLALLTELSQNRSGESCRPFGGSQSGPAFNSIFQKENFQLQLIPPPVTED

Target ORF information:

RefSeq Version	XM_005192219.3
Organism	Bos taurus(cattle)
Definition	Bos taurus VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192219.3

ORF Insert Sequence:

ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TCTGTGCCAA GACTACTGAT 
GAGGAACTGA ACAAGTCTTT CAGTCTAGAA GCTTCACTTT CAAAATTCTC TTATATAGAT 
CTGGACAAGG AACTGGAGTT CAGAAATGAT CTGATTGATG ATAAGGAGTT CGATATTCCT 
CAGGTTGATA CTCCTCCAAC ACTGGAAAGC ATACTAAATG AAACTGATGA TGAAGATGAA 
TCTTTTGTTC TTGAAGATCC TACATTGTTA AACATTGATA CTATTGATTC TCACTCTTAC 
GATACTTCAT CTGTGGCAAG CTCAGATAGT GGTGACAGGA CCAACTTAAA AAGAAAGAAG 
AAATTACCTG ATTCTTTTTC ACTCCATGGA TCAGTTATGC GACATTCACT TTTGAAGGGA 
ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTAGATG CTGGCTTGCC TACAGCAATT 
GCAGTATCCA GTTTGATAGC AGTGGGTACA TCTCATGGAT TGGCCTTAAT ATTTGGAAAA 
GATCAGAATC AAGCTTTGCG ACTCTGCTTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA 
GCTATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCTAAG 
GGACAGATCA CTATGTGGGA TTTGGCCAGT GGAAAGCTTC TAAGATCAAT AACAGATGCT 
CATCCTCCGG GAACAGCAAT ATTACATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT 
TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA 
ACTTGTGAAT CTCGATGCCT GTTTAGCGGT TCCAAGGGTG AAGTTTGCTG TATTGAACCT 
CTCCACTCTA AGCCCGAGTT GAAAGACCAT CCCATCACAC AATTTTCCTT ATTGGCCATG 
GCATCCTTAA CAAAGATACT GGTCATTGGA TTGAAGCCAT CCTTGAAAGT GTGGATGACT 
TTTCCTTATG GACGGATGGA TCCTTCCAGC GTTCCATTGC TGGCCTGGCA CTTCGTGGCA 
GTGCATAATT ACGTGAATCC CATGCTAGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTC 
CTGGTTAAGA GAGACGAATC TGGAGCGATA CATGTTACTA AGCAAAAGCA TCTTCACCTA 
TACTATGACC TCATCAACTT TACTTGGATA AACTCACGCA CAATTGTGCT CTTGGACAGT 
GTAGAGAAGT TGCATGTGAT TGATCGGCAA ACTCAGGAGG AACTGGAAAC AGTGGAGATC 
TCAGAAGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CACTAGCCAC TGGAGGAAAC 
GTGAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGCT ATCAATCCAT AAGTAGCTAT 
GGTGGTCAGA TCTTTTATTT GGGAACAAAA TCTGTTTATG TGATGATGCT GAGGAGCTGG 
AGGGAGAGAG TGGATCACCT CTTGAAACAA GACTGTCTTA CAGAAGCTTT GGCTCTGGCA 
TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTAGGGT TATCAGGGGA TGCCAGTAAG 
CGAAAGGCTA TTGTTGCAGA TCGGATGGTA GAAATCCTAT TGCATTATGC AGACCGAGCT 
CTAAAAAAGT GCCCAGACCA GGGAAAAATC CAAGTGATGG AGCAACATTT TCAGGATATG 
GTACCCGTCA TAGTTGATTA CTGCCTTCTG CTACAGCGAA AGGATCTTTT ATTTAGCCAG 
GTGTATGACA AATTAAATGA GAATTCAGTG GCCAAAGGAG TGTTTCTGGA GTGCCTTGAA 
CCATATATTT TAAGTGATAA ATTGGTGGGG ATCACACCCC AGGTGATGAA AGACTTGATT 
GTTCATTTCC AAGATAAAAA GTTAATGGAA AATGTGGAAG CCCTCATTGT GCATATGGAT 
ATCACCAGCC TAGATATTCA GCAGATAGTT CTCATGTGTT GGGAAAATCG ACTATATGAT 
GCTATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT 
TTCAGAGTCA TTGCACCTCC TTTGAATGCA GGAAAGACGC TAACAGATGA GCAAGTTGTT 
ATGGGCAACA AGCTTCTTGT ATATATTAGC TGTTGTCTAG CAGGTCGTGC CTATCCGCTG 
GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTAATT 
CGCCTGCATT CAACAGAAGC TCCCCCTGAA GAAGAAATCT ATCCTTATAT TCGGACTTTG 
CTGCATTTCG ACACACGAGA ATTTCTAAAT GTATTGGCGC TGACTTTTGA AGATTTTAAA 
AATGATAAGC AAGCTGTAGA ATATCAACAA CGTATTGTGG ATATTTTGTT GAAGGTTATG 
GTGGAGAATT CAGATTTTAC GCCCTCACAA GTGGGGTGTC TCTTTACCTT CCTTGCCCGG 
CAGCTTGCGA AGCCTGACAA CACCTTATTT GTAAACAGAA CTCTTTTTGA TCAGGTCCTT 
GAATTCCTTT GTAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA 
GAATTGCTGC AGGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGACTCAT CCGTATGGCA 
GAGAAAGCAG AATTCTACCA AATTTGTGAA TTTATGTATG AACAAGAACA CCAATATGAC 
AGAATTATTG ATTGCTACTT ACGTGACCCA CTACGAGAGG AAGAAGTCTT TAACTACATT 
CACAATATCT TATCCATTCC TGGATACAGT GTGGAGGAGA AGCAGTCTGT ATGGCAGAAG 
GCTATGGATC ATATTGAGGA ACTTGTGGCC CTGAAGCCCT GTAAAGCTGC AGAGCTGGTT 
GCTACTCACT TTTCTGAACA GATCGAAACA GTCATTAAGA AACTTCAGAA CCAGGTTTTG 
CTTTTCAAGT TTTTAAGAAG CCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA 
TCGCTGCAGA TATCTCCCTG TATAATAGAG CAGTTCATTG AGCTCTTGTG TCAGTTCAGC 
CCAAACCACG TTATTGAGAC GCTGCAAGTC CTTGAGTGCT ACCGCCTAGA AGAAACTATT 
CAGATTACCC AGAAGTATCA ACTTTGTGAA GTCACTGCTT ATCTATTAGA AAAGAAAGGA 
GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGACTAC AAAGCAAACT TCAAGAGATA 
ATACGTCAAG ATAAAAATAC CAAAGAGGAT CCCTCATTGA AGGATGTTGA AGAGACCATG 
GTAGAGACGA TTGCCCTCTG CCAGAGAAAT TCACATAATT TGAACCAACA GCAGAGAGAG 
GCACTCTGGT TTCCGTTATT GGAGGCAATG ATGGCCCCTC AGAAGCTGTC TAGTTCAGCT 
TCACCTCATC CACACTCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT CAACAGTATG 
GCAGCATTTA TTGCCCTTCC ATCCATCCTG CAGCGAATCT TACAGGACCC AGTTTATGGA 
AAAGGAAAAC TTGGAGAAAT CCAAGGCCTT ATTCTGGGAA TGTTGGATAC CTTTAACTAT 
GAACAGACCC TGCTGGAAAC GACAACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG 
TGTAACCTGA GAGCTTCAGT TACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATT 
TGTTTGCAGC AGTACAAGAG ACGCCAAGAA ATGGCTGATG AAATTATTGT CTTTAGCTGT 
GGCCATTTAT ATCACTCGTT CTGTCTGCAA AGCAAAGAGT GCACCATAGA AATTGAAGGG 
CAAACAAGAT GGACGTGCTA CATTTGCATC TCAAGTAACA AAGCAGGAAA GTTGAGTGAA 
AATTCATCTG AAGTTAAAAA GGGAAGGATA ACCCCATCAC AGGCAACCTC AGAACCTGTT 
CTGGATCCAC AGCAAATCCA AGCATTTGAT CAGCTCTGCC ATCTCTACCG AGGAAGTTCC 
AGGCTGGCCC TCCTCACGGA ACTCTCCCAG AATCGCAGTG GCGAGAGCTG CAGGCCATTT 
GGAGGGTCCC AGAGTGGCCC CGCCTTCAAC AGCATCTTCC AGAAGGAGAA CTTCCAGCTG 
CAGCTCATTC CTCCCCCGGT GACTGAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	VPS8
Entrez Gene ID	615672
Full Name	VPS8, CORVET complex subunit
General protein information	Preferred Names VPS8, CORVET complex subunit Names vacuolar protein sorting-associated protein 8 homolog Vacuolar protein sorting-associated protein 8 homolog vacuolar protein sorting 8 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	VPS8	XP_001097319.1
Mus musculus (house mouse)	Vps8	NP_001272822.1
Xenopus tropicalis (tropical clawed frog)	vps8	XP_002936581.2
Pan troglodytes (chimpanzee)	VPS8	XP_003310234.1
Gallus gallus (chicken)	VPS8	XP_004937039.1
Caenorhabditis elegans (roundworm)	C42C1.4	NP_001023059.1
Danio rerio (zebrafish)	vps8	NP_001074085.2
Drosophila melanogaster (fruit fly)	CG10144	NP_648048.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007578	XP_308296.3
Arabidopsis thaliana (thale cress)	AT4G00800	NP_567189.6
Bos taurus (cattle)	VPS8	XP_003585710.1
Rattus norvegicus (Norway rat)	Vps8	XP_006248637.1
Homo sapiens (human)	VPS8	NP_001009921.1
Canis lupus familiaris (dog)	VPS8	XP_005639847.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

VPS8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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