TRMT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT1
Entrez Gene ID	539613
Full Name	tRNA methyltransferase 1
General protein information	Preferred Names tRNA methyltransferase 1 Names tRNA (guanine(26)-N(2))-dimethyltransferase TRM1 tRNA methyltransferase 1 homolog tRNA methyltransferase 1 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015471950.1	XP_015327436.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_015471951.1	XP_015327437.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
XM_015471951.2	XP_015327437.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
NM_001206374.1	NP_001193303.1	tRNA (guanine(26)-N(2))-dimethyltransferase
XM_024994483.1	XP_024850251.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1

Pathways from BioSystems

Homologies

	TRMT1	XP_002808218.1
Mus musculus (house mouse)	Trmt1	NP_932137.2
Caenorhabditis elegans (roundworm)	trm-1	NP_506513.1
Xenopus tropicalis (tropical clawed frog)	trmt1	XP_004919202.1
Danio rerio (zebrafish)	trmt1	XP_685886.4
Kluyveromyces lactis	KLLA0F19404g	XP_455951.1
Neurospora crassa	NCU06336	XP_962370.1
Oryza sativa (rice)	Os03g0786700	NP_001051491.1
Rattus norvegicus (Norway rat)	Trmt1	NP_001013892.1
Drosophila melanogaster (fruit fly)	CG6388	NP_609566.1
Saccharomyces cerevisiae (baker's yeast)	TRM1	NP_010405.3
Schizosaccharomyces pombe (fission yeast)	trm1	NP_596547.1
Magnaporthe oryzae (rice blast fungus)	MGG_01448	XP_003714401.1
Homo sapiens (human)	TRMT1	NP_060192.1
Bos taurus (cattle)	TRMT1	NP_001193303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009740	XP_318825.4
Arabidopsis thaliana (thale cress)	AT5G15810	NP_197085.2
Pan troglodytes (chimpanzee)	TRMT1	XP_512420.1
Canis lupus familiaris (dog)	TRMT1	XP_533897.5
Eremothecium gossypii	AGOS_AGR075W	NP_986740.1
Arabidopsis thaliana (thale cress)	AT3G02320	NP_186881.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38007	NM_001206374.1 Latest version!	Bos taurus tRNA methyltransferase 1 (TRMT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa20922	XM_015471950.1 Latest version!	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa20922	XM_024994483.1 Latest version!	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa20923	XM_015471951.2 Latest version!	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa20923	XM_015471951.1	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT1 ( NM_001206374.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193303.1 Bos taurus tRNA methyltransferase 1 (TRMT1), mRNA.

Price & Availability↑

CloneID	OBa38007
Clone ID Related Accession (Same CDS sequence)	NM_001206374.1
Accession Version	NM_001206374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC149091.1. On or before Apr 23, 2011 this sequence version replaced XM_588358.5, XM_002688756.1. ##Evidence-Data-START## Transcript exon combination :: BC149091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCGTG CGAGGACCGT TCTGTGGCTA AGCCCGACTC TCCGCTCCGT TCTCAGTCTC 
TATAGAGCCC GGTTTATGGA GGGGCAGCCC CAAGGGCCGC CAAATTCAGC AGCCATGGAG 
AATGGCACCG AGCCGGACGG AGAAGAGCGC CCACCTGGGT TCCAGGAGAC GACGATCACC 
GAGGGGGCCG CCAGGATCGT CTTTCCCAAC GCCAACGAAG TTTTCTACAA CCCAGTGCAA 
GAGTTCAACC GGGACCTAAC ATGCGCTGTG ATCACGGAAT TTGCTCGTAT TCAGCTTGCG 
GCCAAAGGAA TCCAGATCAA GGTGCCAGGT GAGAAGGATG TGCAAAAGGT GGTCGTGGAC 
TTGTCAGAGC CAACGGAGGA CAAGGCTGAA CTGAAAGAGG GTGCAAACCT GGCCCTGGAA 
GACCAGCCTC GAACAGCCGC CGTGGGGGAG ATCTGTGAGG AAGGCCTGCG AGTGCTGGAA 
GGCCTGGCAG CCTCCGGCTT ACGTTCCATT CGCTTTGCTC GAGAGGTGCC TGGTCTCCGA 
TCCGTGGTTG CCAATGACGC CTCTGCCCGA GCTGTGGATC TTATGCGCCG TAATGTGCAG 
CTCAACGAAG TGGCCCACCT GGTACAGCCC AGCCAGGCAG ATGCCCGGAT GCTGATGTAC 
CAGCACCAGA AGGCGTCGGA GAGGTTTGAC GTCATCGACC TCGACCCTTA CGGCAGCCCC 
GCCTCTTTCC TGGATGCAGC CGTGCAGGCT GTGAGTGAAG GAGGGCTGCT GTGCGTCACC 
TGCACAGATA TGGCGGTCCT GGCGGGGAAC AGCGGGGAGA CCTGCTACAG CAAATACGGG 
GCCATGGCCC TCAAGAGCCG GGCCTGCCAC GAGATGGCCC TGAGGACTGT CCTGCACAGC 
CTGGATCTCC GTGCCAACTG CTACCAGCGC TTTGTGGTGC CACTGCTCAG CATCAGCGCT 
GACTTCTATG TACGGGTTTT TGTTCGTGTC TTCACTGGTC AGGCCAAGGT CAAGGCCTCA 
GCCAGCAAGC AGGCGCTGGT GTTCCAGTGC GTGGGCTGCG GGGCCTTCCA CCTGCAGCGT 
CTTGGCAAAG CATCAGCAGC CTCCGGTGGC CGGCTCAAGT TCTCTGCAGC CTGCGGCCCC 
CCCGTGGCCC CTGAGTGCGA GCACTGTGGG CAGCGACACC AGCTTGGTGG CCCCATGTGG 
GCAGAGCCCC TCCATGACCT GGAATTCGTG GGCCGTGTCC TCGAGGCTGT GAGCGCCAAC 
CCTGGCCGCT TCCACACCGC AGAGCGGATC CGAGGAGTGC TGAGTGTCAT CACGGAGGAG 
CTCCCGGACG TGCCTCTCTA CTATACGCTG GACCAGCTGA GCAGCACCAT CCACTGCAAC 
ACACCCAGCC TCCTGCAGCT GCGGTCGGCC CTCCTCCACG CTGGCTTCCG GGTCTCCCTC 
TCCCATGCCT GTAAGAATGC TGTGAAGACA GACGCTCCCT CCTCGACCCT CTGGGACATC 
ATGCGGTGCT GGGAGAAGGA GTGTCCAGTG AAACGGGAGC GCCTGTCAGA GAGCAGCCCA 
GCCTTCCGCA TTCTCAGCGT GGAGCCCAGG CTGCAGGCCA ACTTCACCAT CCGGGATGAT 
GCCAACCCCA GCTCCCGCCA GCGAGGACTC AAGCGCTTCC AGGCCAACCC TGAGGCCAAC 
TGGGGTCCCC GGCCCCGTGC CCGGCCAGGA GGCAAGGCAG CAGGAGAAAC TGTGGAAGAG 
AGACGTAGGC TGCTCCAAAA TAAGCGAAAA GAGCCGGTGG AGGACCCAGC TGAGCGGGCT 
GCCTGGCTCA AAACATTTCC CTGCAAGAGG TTCAAGGAGG GCACCTGTCA ACAGGGGGGC 
CAGTGCTGCT ACTCGCATAG CCCCCCCTCA CCTAAGGCCA CTGCTGAAGC CACCCCCACT 
GACTGTCCAG AGGCCCCCAG TCAGAACCCC GCTGAGCCTG GGGCTGCTAC TGGTCCAGGC 
ACAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193303.1
CDS	88..2076
Translation	MSRARTVLWLSPTLRSVLSLYRARFMEGQPQGPPNSAAMENGTEPDGEERPPGFQETTITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKVVVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPYGSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASAASGGRLKFSAACGPPVAPECEHCGQRHQLGGPMWAEPLHDLEFVGRVLEAVSANPGRFHTAERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHAGFRVSLSHACKNAVKTDAPSSTLWDIMRCWEKECPVKRERLSESSPAFRILSVEPRLQANFTIRDDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAAGETVEERRRLLQNKRKEPVEDPAERAAWLKTFPCKRFKEGTCQQGGQCCYSHSPPSPKATAEATPTDCPEAPSQNPAEPGAATGPGTE

Target ORF information:

RefSeq Version	NM_001206374.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 (TRMT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206374.1

ORF Insert Sequence:

ATGTCCCGTG CGAGGACCGT TCTGTGGCTA AGCCCGACTC TCCGCTCCGT TCTCAGTCTC 
TATAGAGCCC GGTTTATGGA GGGGCAGCCC CAAGGGCCGC CAAATTCAGC AGCCATGGAG 
AATGGCACCG AGCCGGACGG AGAAGAGCGC CCACCTGGGT TCCAGGAGAC GACGATCACC 
GAGGGGGCCG CCAGGATCGT CTTTCCCAAC GCCAACGAAG TTTTCTACAA CCCAGTGCAA 
GAGTTCAACC GGGACCTAAC ATGCGCTGTG ATCACGGAAT TTGCTCGTAT TCAGCTTGCG 
GCCAAAGGAA TCCAGATCAA GGTGCCAGGT GAGAAGGATG TGCAAAAGGT GGTCGTGGAC 
TTGTCAGAGC CAACGGAGGA CAAGGCTGAA CTGAAAGAGG GTGCAAACCT GGCCCTGGAA 
GACCAGCCTC GAACAGCCGC CGTGGGGGAG ATCTGTGAGG AAGGCCTGCG AGTGCTGGAA 
GGCCTGGCAG CCTCCGGCTT ACGTTCCATT CGCTTTGCTC GAGAGGTGCC TGGTCTCCGA 
TCCGTGGTTG CCAATGACGC CTCTGCCCGA GCTGTGGATC TTATGCGCCG TAATGTGCAG 
CTCAACGAAG TGGCCCACCT GGTACAGCCC AGCCAGGCAG ATGCCCGGAT GCTGATGTAC 
CAGCACCAGA AGGCGTCGGA GAGGTTTGAC GTCATCGACC TCGACCCTTA CGGCAGCCCC 
GCCTCTTTCC TGGATGCAGC CGTGCAGGCT GTGAGTGAAG GAGGGCTGCT GTGCGTCACC 
TGCACAGATA TGGCGGTCCT GGCGGGGAAC AGCGGGGAGA CCTGCTACAG CAAATACGGG 
GCCATGGCCC TCAAGAGCCG GGCCTGCCAC GAGATGGCCC TGAGGACTGT CCTGCACAGC 
CTGGATCTCC GTGCCAACTG CTACCAGCGC TTTGTGGTGC CACTGCTCAG CATCAGCGCT 
GACTTCTATG TACGGGTTTT TGTTCGTGTC TTCACTGGTC AGGCCAAGGT CAAGGCCTCA 
GCCAGCAAGC AGGCGCTGGT GTTCCAGTGC GTGGGCTGCG GGGCCTTCCA CCTGCAGCGT 
CTTGGCAAAG CATCAGCAGC CTCCGGTGGC CGGCTCAAGT TCTCTGCAGC CTGCGGCCCC 
CCCGTGGCCC CTGAGTGCGA GCACTGTGGG CAGCGACACC AGCTTGGTGG CCCCATGTGG 
GCAGAGCCCC TCCATGACCT GGAATTCGTG GGCCGTGTCC TCGAGGCTGT GAGCGCCAAC 
CCTGGCCGCT TCCACACCGC AGAGCGGATC CGAGGAGTGC TGAGTGTCAT CACGGAGGAG 
CTCCCGGACG TGCCTCTCTA CTATACGCTG GACCAGCTGA GCAGCACCAT CCACTGCAAC 
ACACCCAGCC TCCTGCAGCT GCGGTCGGCC CTCCTCCACG CTGGCTTCCG GGTCTCCCTC 
TCCCATGCCT GTAAGAATGC TGTGAAGACA GACGCTCCCT CCTCGACCCT CTGGGACATC 
ATGCGGTGCT GGGAGAAGGA GTGTCCAGTG AAACGGGAGC GCCTGTCAGA GAGCAGCCCA 
GCCTTCCGCA TTCTCAGCGT GGAGCCCAGG CTGCAGGCCA ACTTCACCAT CCGGGATGAT 
GCCAACCCCA GCTCCCGCCA GCGAGGACTC AAGCGCTTCC AGGCCAACCC TGAGGCCAAC 
TGGGGTCCCC GGCCCCGTGC CCGGCCAGGA GGCAAGGCAG CAGGAGAAAC TGTGGAAGAG 
AGACGTAGGC TGCTCCAAAA TAAGCGAAAA GAGCCGGTGG AGGACCCAGC TGAGCGGGCT 
GCCTGGCTCA AAACATTTCC CTGCAAGAGG TTCAAGGAGG GCACCTGTCA ACAGGGGGGC 
CAGTGCTGCT ACTCGCATAG CCCCCCCTCA CCTAAGGCCA CTGCTGAAGC CACCCCCACT 
GACTGTCCAG AGGCCCCCAG TCAGAACCCC GCTGAGCCTG GGGCTGCTAC TGGTCCAGGC 
ACAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_015471950.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327436.1 Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20922
Clone ID Related Accession (Same CDS sequence)	XM_015471950.1 , XM_024994483.1
Accession Version	XM_015471950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104012.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGGA CTGGCGGGCA GAGGCGCGCG 
CGGATGTCCC GTGCGAGGAC CGTTCTGTGG CTAAGCCCGA CTCTCCGCTC CGTTCTCAGT 
CTCTATAGAG CCCGGTTTAT GGAGGGGCAG CCCCAAGGGC CGCCAAATTC AGCAGCCATG 
GAGAATGGCA CCGAGCCGGA CGGAGAAGAG CGCCCACCTG GGTTCCAGGA GACGACGATC 
ACCGAGGGGG CCGCCAGGAT CGTCTTTCCC AACGCCAACG AAGTTTTCTA CAACCCAGTG 
CAAGAGTTCA ACCGGGACCT AACATGCGCT GTGATCACGG AATTTGCTCG TATTCAGCTT 
GCGGCCAAAG GAATCCAGAT CAAGGTGCCA GGTGAGAAGG ATGTGCAAAA GGTGGTCGTG 
GACTTGTCAG AGCCAACGGA GGACAAGGCT GAACTGAAAG AGGGTGCAAA CCTGGCCCTG 
GAAGACCAGC CTCGAACAGC CGCCGTGGGG GAGATCTGTG AGGAAGGCCT GCGAGTGCTG 
GAAGGCCTGG CAGCCTCCGG CTTACGTTCC ATTCGCTTTG CTCGAGAGGT GCCTGGTCTC 
CGATCCGTGG TTGCCAATGA CGCCTCTGCC CGAGCTGTGG ATCTTATGCG CCGTAATGTG 
CAGCTCAACG AAGTGGCCCA CCTGGTACAG CCCAGCCAGG CAGATGCCCG GATGCTGATG 
TACCAGCACC AGAAGGCGTC GGAGAGGTTT GACGTCATCG ACCTCGACCC TTACGGCAGC 
CCCGCCTCTT TCCTGGATGC AGCCGTGCAG GCTGTGAGTG AAGGAGGGCT GCTGTGCGTC 
ACCTGCACAG ATATGGCGGT CCTGGCGGGG AACAGCGGGG AGACCTGCTA CAGCAAATAC 
GGGGCCATGG CCCTCAAGAG CCGGGCCTGC CACGAGATGG CCCTGAGGAC TGTCCTGCAC 
AGCCTGGATC TCCGTGCCAA CTGCTACCAG CGCTTTGTGG TGCCACTGCT CAGCATCAGC 
GCTGACTTCT ATGTACGGGT TTTTGTTCGT GTCTTCACTG GTCAGGCCAA GGTCAAGGCC 
TCAGCCAGCA AGCAGGCGCT GGTGTTCCAG TGCGTGGGCT GCGGGGCCTT CCACCTGCAG 
CGTCTTGGCA AAGCATCAGC AGCCTCCGGT GGCCGGCTCA AGTTCTCTGC AGCCTGCGGC 
CCCCCCGTGG CCCCTGAGTG CGAGCACTGT GGGCAGCGAC ACCAGCTTGG TGGCCCCATG 
TGGGCAGAGC CCCTCCATGA CCTGGAATTC GTGGGCCGTG TCCTCGAGGC TGTGAGCGCC 
AACCCTGGCC GCTTCCACAC CGCAGAGCGG ATCCGAGGAG TGCTGAGTGT CATCACGGAG 
GAGCTCCCGG ACGTGCCTCT CTACTATACG CTGGACCAGC TGAGCAGCAC CATCCACTGC 
AACACACCCA GCCTCCTGCA GCTGCGGTCG GCCCTCCTCC ACGCTGGCTT CCGGGTCTCC 
CTCTCCCATG CCTGTAAGAA TGCTGTGAAG ACAGACGCTC CCTCCTCGAC CCTCTGGGAC 
ATCATGCGGT GCTGGGAGAA GGAGTGTCCA GTGAAACGGG AGCGCCTGTC AGAGAGCAGC 
CCAGCCTTCC GCATTCTCAG CGTGGAGCCC AGGCTGCAGG CCAACTTCAC CATCCGGGAT 
GATGCCAACC CCAGCTCCCG CCAGCGAGGA CTCAAGCGCT TCCAGGCCAA CCCTGAGGCC 
AACTGGGGTC CCCGGCCCCG TGCCCGGCCA GGAGGCAAGG CAGCAGGAGA AACTGTGGAA 
GAGAGACGTA GGCTGCTCCA AAATAAGCGA AAAGAGCCGG TGGAGGACCC AGCTGAGCGG 
GCTGCCTGGC TCAAAACATT TCCCTGCAAG AGGTTCAAGG AGGGCACCTG TCAACAGGGG 
GGCCAGTGCT GCTACTCGCA TAGCCCCCCC TCACCTAAGG CCACTGCTGA AGCCACCCCC 
ACTGACTGTC CAGAGGCCCC CAGTCAGAAC CCCGCTGAGC CTGGGGCTGC TACTGGTCCA 
GGCACAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327436.1
CDS	16..2127
Translation	MICPPSVPTCRRPGPNPASVHRDQFDFRCLERRTGGQRRARMSRARTVLWLSPTLRSVLSLYRARFMEGQPQGPPNSAAMENGTEPDGEERPPGFQETTITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKVVVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPYGSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASAASGGRLKFSAACGPPVAPECEHCGQRHQLGGPMWAEPLHDLEFVGRVLEAVSANPGRFHTAERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHAGFRVSLSHACKNAVKTDAPSSTLWDIMRCWEKECPVKRERLSESSPAFRILSVEPRLQANFTIRDDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAAGETVEERRRLLQNKRKEPVEDPAERAAWLKTFPCKRFKEGTCQQGGQCCYSHSPPSPKATAEATPTDCPEAPSQNPAEPGAATGPGTE

Target ORF information:

RefSeq Version	XM_015471950.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471950.1

ORF Insert Sequence:

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGGA CTGGCGGGCA GAGGCGCGCG 
CGGATGTCCC GTGCGAGGAC CGTTCTGTGG CTAAGCCCGA CTCTCCGCTC CGTTCTCAGT 
CTCTATAGAG CCCGGTTTAT GGAGGGGCAG CCCCAAGGGC CGCCAAATTC AGCAGCCATG 
GAGAATGGCA CCGAGCCGGA CGGAGAAGAG CGCCCACCTG GGTTCCAGGA GACGACGATC 
ACCGAGGGGG CCGCCAGGAT CGTCTTTCCC AACGCCAACG AAGTTTTCTA CAACCCAGTG 
CAAGAGTTCA ACCGGGACCT AACATGCGCT GTGATCACGG AATTTGCTCG TATTCAGCTT 
GCGGCCAAAG GAATCCAGAT CAAGGTGCCA GGTGAGAAGG ATGTGCAAAA GGTGGTCGTG 
GACTTGTCAG AGCCAACGGA GGACAAGGCT GAACTGAAAG AGGGTGCAAA CCTGGCCCTG 
GAAGACCAGC CTCGAACAGC CGCCGTGGGG GAGATCTGTG AGGAAGGCCT GCGAGTGCTG 
GAAGGCCTGG CAGCCTCCGG CTTACGTTCC ATTCGCTTTG CTCGAGAGGT GCCTGGTCTC 
CGATCCGTGG TTGCCAATGA CGCCTCTGCC CGAGCTGTGG ATCTTATGCG CCGTAATGTG 
CAGCTCAACG AAGTGGCCCA CCTGGTACAG CCCAGCCAGG CAGATGCCCG GATGCTGATG 
TACCAGCACC AGAAGGCGTC GGAGAGGTTT GACGTCATCG ACCTCGACCC TTACGGCAGC 
CCCGCCTCTT TCCTGGATGC AGCCGTGCAG GCTGTGAGTG AAGGAGGGCT GCTGTGCGTC 
ACCTGCACAG ATATGGCGGT CCTGGCGGGG AACAGCGGGG AGACCTGCTA CAGCAAATAC 
GGGGCCATGG CCCTCAAGAG CCGGGCCTGC CACGAGATGG CCCTGAGGAC TGTCCTGCAC 
AGCCTGGATC TCCGTGCCAA CTGCTACCAG CGCTTTGTGG TGCCACTGCT CAGCATCAGC 
GCTGACTTCT ATGTACGGGT TTTTGTTCGT GTCTTCACTG GTCAGGCCAA GGTCAAGGCC 
TCAGCCAGCA AGCAGGCGCT GGTGTTCCAG TGCGTGGGCT GCGGGGCCTT CCACCTGCAG 
CGTCTTGGCA AAGCATCAGC AGCCTCCGGT GGCCGGCTCA AGTTCTCTGC AGCCTGCGGC 
CCCCCCGTGG CCCCTGAGTG CGAGCACTGT GGGCAGCGAC ACCAGCTTGG TGGCCCCATG 
TGGGCAGAGC CCCTCCATGA CCTGGAATTC GTGGGCCGTG TCCTCGAGGC TGTGAGCGCC 
AACCCTGGCC GCTTCCACAC CGCAGAGCGG ATCCGAGGAG TGCTGAGTGT CATCACGGAG 
GAGCTCCCGG ACGTGCCTCT CTACTATACG CTGGACCAGC TGAGCAGCAC CATCCACTGC 
AACACACCCA GCCTCCTGCA GCTGCGGTCG GCCCTCCTCC ACGCTGGCTT CCGGGTCTCC 
CTCTCCCATG CCTGTAAGAA TGCTGTGAAG ACAGACGCTC CCTCCTCGAC CCTCTGGGAC 
ATCATGCGGT GCTGGGAGAA GGAGTGTCCA GTGAAACGGG AGCGCCTGTC AGAGAGCAGC 
CCAGCCTTCC GCATTCTCAG CGTGGAGCCC AGGCTGCAGG CCAACTTCAC CATCCGGGAT 
GATGCCAACC CCAGCTCCCG CCAGCGAGGA CTCAAGCGCT TCCAGGCCAA CCCTGAGGCC 
AACTGGGGTC CCCGGCCCCG TGCCCGGCCA GGAGGCAAGG CAGCAGGAGA AACTGTGGAA 
GAGAGACGTA GGCTGCTCCA AAATAAGCGA AAAGAGCCGG TGGAGGACCC AGCTGAGCGG 
GCTGCCTGGC TCAAAACATT TCCCTGCAAG AGGTTCAAGG AGGGCACCTG TCAACAGGGG 
GGCCAGTGCT GCTACTCGCA TAGCCCCCCC TCACCTAAGG CCACTGCTGA AGCCACCCCC 
ACTGACTGTC CAGAGGCCCC CAGTCAGAAC CCCGCTGAGC CTGGGGCTGC TACTGGTCCA 
GGCACAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024994483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850251.1 Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20922
Clone ID Related Accession (Same CDS sequence)	XM_015471950.1 , XM_024994483.1
Accession Version	XM_024994483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGGA CTGGCGGGCA GAGGCGCGCG 
CGGATGTCCC GTGCGAGGAC CGTTCTGTGG CTAAGCCCGA CTCTCCGCTC CGTTCTCAGT 
CTCTATAGAG CCCGGTTTAT GGAGGGGCAG CCCCAAGGGC CGCCAAATTC AGCAGCCATG 
GAGAATGGCA CCGAGCCGGA CGGAGAAGAG CGCCCACCTG GGTTCCAGGA GACGACGATC 
ACCGAGGGGG CCGCCAGGAT CGTCTTTCCC AACGCCAACG AAGTTTTCTA CAACCCAGTG 
CAAGAGTTCA ACCGGGACCT AACATGCGCT GTGATCACGG AATTTGCTCG TATTCAGCTT 
GCGGCCAAAG GAATCCAGAT CAAGGTGCCA GGTGAGAAGG ATGTGCAAAA GGTGGTCGTG 
GACTTGTCAG AGCCAACGGA GGACAAGGCT GAACTGAAAG AGGGTGCAAA CCTGGCCCTG 
GAAGACCAGC CTCGAACAGC CGCCGTGGGG GAGATCTGTG AGGAAGGCCT GCGAGTGCTG 
GAAGGCCTGG CAGCCTCCGG CTTACGTTCC ATTCGCTTTG CTCGAGAGGT GCCTGGTCTC 
CGATCCGTGG TTGCCAATGA CGCCTCTGCC CGAGCTGTGG ATCTTATGCG CCGTAATGTG 
CAGCTCAACG AAGTGGCCCA CCTGGTACAG CCCAGCCAGG CAGATGCCCG GATGCTGATG 
TACCAGCACC AGAAGGCGTC GGAGAGGTTT GACGTCATCG ACCTCGACCC TTACGGCAGC 
CCCGCCTCTT TCCTGGATGC AGCCGTGCAG GCTGTGAGTG AAGGAGGGCT GCTGTGCGTC 
ACCTGCACAG ATATGGCGGT CCTGGCGGGG AACAGCGGGG AGACCTGCTA CAGCAAATAC 
GGGGCCATGG CCCTCAAGAG CCGGGCCTGC CACGAGATGG CCCTGAGGAC TGTCCTGCAC 
AGCCTGGATC TCCGTGCCAA CTGCTACCAG CGCTTTGTGG TGCCACTGCT CAGCATCAGC 
GCTGACTTCT ATGTACGGGT TTTTGTTCGT GTCTTCACTG GTCAGGCCAA GGTCAAGGCC 
TCAGCCAGCA AGCAGGCGCT GGTGTTCCAG TGCGTGGGCT GCGGGGCCTT CCACCTGCAG 
CGTCTTGGCA AAGCATCAGC AGCCTCCGGT GGCCGGCTCA AGTTCTCTGC AGCCTGCGGC 
CCCCCCGTGG CCCCTGAGTG CGAGCACTGT GGGCAGCGAC ACCAGCTTGG TGGCCCCATG 
TGGGCAGAGC CCCTCCATGA CCTGGAATTC GTGGGCCGTG TCCTCGAGGC TGTGAGCGCC 
AACCCTGGCC GCTTCCACAC CGCAGAGCGG ATCCGAGGAG TGCTGAGTGT CATCACGGAG 
GAGCTCCCGG ACGTGCCTCT CTACTATACG CTGGACCAGC TGAGCAGCAC CATCCACTGC 
AACACACCCA GCCTCCTGCA GCTGCGGTCG GCCCTCCTCC ACGCTGGCTT CCGGGTCTCC 
CTCTCCCATG CCTGTAAGAA TGCTGTGAAG ACAGACGCTC CCTCCTCGAC CCTCTGGGAC 
ATCATGCGGT GCTGGGAGAA GGAGTGTCCA GTGAAACGGG AGCGCCTGTC AGAGAGCAGC 
CCAGCCTTCC GCATTCTCAG CGTGGAGCCC AGGCTGCAGG CCAACTTCAC CATCCGGGAT 
GATGCCAACC CCAGCTCCCG CCAGCGAGGA CTCAAGCGCT TCCAGGCCAA CCCTGAGGCC 
AACTGGGGTC CCCGGCCCCG TGCCCGGCCA GGAGGCAAGG CAGCAGGAGA AACTGTGGAA 
GAGAGACGTA GGCTGCTCCA AAATAAGCGA AAAGAGCCGG TGGAGGACCC AGCTGAGCGG 
GCTGCCTGGC TCAAAACATT TCCCTGCAAG AGGTTCAAGG AGGGCACCTG TCAACAGGGG 
GGCCAGTGCT GCTACTCGCA TAGCCCCCCC TCACCTAAGG CCACTGCTGA AGCCACCCCC 
ACTGACTGTC CAGAGGCCCC CAGTCAGAAC CCCGCTGAGC CTGGGGCTGC TACTGGTCCA 
GGCACAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850251.1
CDS	414..2525
Translation	MICPPSVPTCRRPGPNPASVHRDQFDFRCLERRTGGQRRARMSRARTVLWLSPTLRSVLSLYRARFMEGQPQGPPNSAAMENGTEPDGEERPPGFQETTITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKVVVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPYGSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASAASGGRLKFSAACGPPVAPECEHCGQRHQLGGPMWAEPLHDLEFVGRVLEAVSANPGRFHTAERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHAGFRVSLSHACKNAVKTDAPSSTLWDIMRCWEKECPVKRERLSESSPAFRILSVEPRLQANFTIRDDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAAGETVEERRRLLQNKRKEPVEDPAERAAWLKTFPCKRFKEGTCQQGGQCCYSHSPPSPKATAEATPTDCPEAPSQNPAEPGAATGPGTE

Target ORF information:

RefSeq Version	XM_024994483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994483.1

ORF Insert Sequence:

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGGA CTGGCGGGCA GAGGCGCGCG 
CGGATGTCCC GTGCGAGGAC CGTTCTGTGG CTAAGCCCGA CTCTCCGCTC CGTTCTCAGT 
CTCTATAGAG CCCGGTTTAT GGAGGGGCAG CCCCAAGGGC CGCCAAATTC AGCAGCCATG 
GAGAATGGCA CCGAGCCGGA CGGAGAAGAG CGCCCACCTG GGTTCCAGGA GACGACGATC 
ACCGAGGGGG CCGCCAGGAT CGTCTTTCCC AACGCCAACG AAGTTTTCTA CAACCCAGTG 
CAAGAGTTCA ACCGGGACCT AACATGCGCT GTGATCACGG AATTTGCTCG TATTCAGCTT 
GCGGCCAAAG GAATCCAGAT CAAGGTGCCA GGTGAGAAGG ATGTGCAAAA GGTGGTCGTG 
GACTTGTCAG AGCCAACGGA GGACAAGGCT GAACTGAAAG AGGGTGCAAA CCTGGCCCTG 
GAAGACCAGC CTCGAACAGC CGCCGTGGGG GAGATCTGTG AGGAAGGCCT GCGAGTGCTG 
GAAGGCCTGG CAGCCTCCGG CTTACGTTCC ATTCGCTTTG CTCGAGAGGT GCCTGGTCTC 
CGATCCGTGG TTGCCAATGA CGCCTCTGCC CGAGCTGTGG ATCTTATGCG CCGTAATGTG 
CAGCTCAACG AAGTGGCCCA CCTGGTACAG CCCAGCCAGG CAGATGCCCG GATGCTGATG 
TACCAGCACC AGAAGGCGTC GGAGAGGTTT GACGTCATCG ACCTCGACCC TTACGGCAGC 
CCCGCCTCTT TCCTGGATGC AGCCGTGCAG GCTGTGAGTG AAGGAGGGCT GCTGTGCGTC 
ACCTGCACAG ATATGGCGGT CCTGGCGGGG AACAGCGGGG AGACCTGCTA CAGCAAATAC 
GGGGCCATGG CCCTCAAGAG CCGGGCCTGC CACGAGATGG CCCTGAGGAC TGTCCTGCAC 
AGCCTGGATC TCCGTGCCAA CTGCTACCAG CGCTTTGTGG TGCCACTGCT CAGCATCAGC 
GCTGACTTCT ATGTACGGGT TTTTGTTCGT GTCTTCACTG GTCAGGCCAA GGTCAAGGCC 
TCAGCCAGCA AGCAGGCGCT GGTGTTCCAG TGCGTGGGCT GCGGGGCCTT CCACCTGCAG 
CGTCTTGGCA AAGCATCAGC AGCCTCCGGT GGCCGGCTCA AGTTCTCTGC AGCCTGCGGC 
CCCCCCGTGG CCCCTGAGTG CGAGCACTGT GGGCAGCGAC ACCAGCTTGG TGGCCCCATG 
TGGGCAGAGC CCCTCCATGA CCTGGAATTC GTGGGCCGTG TCCTCGAGGC TGTGAGCGCC 
AACCCTGGCC GCTTCCACAC CGCAGAGCGG ATCCGAGGAG TGCTGAGTGT CATCACGGAG 
GAGCTCCCGG ACGTGCCTCT CTACTATACG CTGGACCAGC TGAGCAGCAC CATCCACTGC 
AACACACCCA GCCTCCTGCA GCTGCGGTCG GCCCTCCTCC ACGCTGGCTT CCGGGTCTCC 
CTCTCCCATG CCTGTAAGAA TGCTGTGAAG ACAGACGCTC CCTCCTCGAC CCTCTGGGAC 
ATCATGCGGT GCTGGGAGAA GGAGTGTCCA GTGAAACGGG AGCGCCTGTC AGAGAGCAGC 
CCAGCCTTCC GCATTCTCAG CGTGGAGCCC AGGCTGCAGG CCAACTTCAC CATCCGGGAT 
GATGCCAACC CCAGCTCCCG CCAGCGAGGA CTCAAGCGCT TCCAGGCCAA CCCTGAGGCC 
AACTGGGGTC CCCGGCCCCG TGCCCGGCCA GGAGGCAAGG CAGCAGGAGA AACTGTGGAA 
GAGAGACGTA GGCTGCTCCA AAATAAGCGA AAAGAGCCGG TGGAGGACCC AGCTGAGCGG 
GCTGCCTGGC TCAAAACATT TCCCTGCAAG AGGTTCAAGG AGGGCACCTG TCAACAGGGG 
GGCCAGTGCT GCTACTCGCA TAGCCCCCCC TCACCTAAGG CCACTGCTGA AGCCACCCCC 
ACTGACTGTC CAGAGGCCCC CAGTCAGAAC CCCGCTGAGC CTGGGGCTGC TACTGGTCCA 
GGCACAGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_015471951.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327437.1 Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

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CloneID	OBa20923
Clone ID Related Accession (Same CDS sequence)	XM_015471951.1 , XM_015471951.2
Accession Version	XM_015471951.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGTC TCTATAGAGC CCGGTTTATG 
GAGGGGCAGC CCCAAGGGCC GCCAAATTCA GCAGCCATGG AGAATGGCAC CGAGCCGGAC 
GGAGAAGAGC GCCCACCTGG GTTCCAGGAG ACGACGATCA CCGAGGGGGC CGCCAGGATC 
GTCTTTCCCA ACGCCAACGA AGTTTTCTAC AACCCAGTGC AAGAGTTCAA CCGGGACCTA 
ACATGCGCTG TGATCACGGA ATTTGCTCGT ATTCAGCTTG CGGCCAAAGG AATCCAGATC 
AAGGTGCCAG GTGAGAAGGA TGTGCAAAAG GTGGTCGTGG ACTTGTCAGA GCCAACGGAG 
GACAAGGCTG AACTGAAAGA GGGTGCAAAC CTGGCCCTGG AAGACCAGCC TCGAACAGCC 
GCCGTGGGGG AGATCTGTGA GGAAGGCCTG CGAGTGCTGG AAGGCCTGGC AGCCTCCGGC 
TTACGTTCCA TTCGCTTTGC TCGAGAGGTG CCTGGTCTCC GATCCGTGGT TGCCAATGAC 
GCCTCTGCCC GAGCTGTGGA TCTTATGCGC CGTAATGTGC AGCTCAACGA AGTGGCCCAC 
CTGGTACAGC CCAGCCAGGC AGATGCCCGG ATGCTGATGT ACCAGCACCA GAAGGCGTCG 
GAGAGGTTTG ACGTCATCGA CCTCGACCCT TACGGCAGCC CCGCCTCTTT CCTGGATGCA 
GCCGTGCAGG CTGTGAGTGA AGGAGGGCTG CTGTGCGTCA CCTGCACAGA TATGGCGGTC 
CTGGCGGGGA ACAGCGGGGA GACCTGCTAC AGCAAATACG GGGCCATGGC CCTCAAGAGC 
CGGGCCTGCC ACGAGATGGC CCTGAGGACT GTCCTGCACA GCCTGGATCT CCGTGCCAAC 
TGCTACCAGC GCTTTGTGGT GCCACTGCTC AGCATCAGCG CTGACTTCTA TGTACGGGTT 
TTTGTTCGTG TCTTCACTGG TCAGGCCAAG GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG 
GTGTTCCAGT GCGTGGGCTG CGGGGCCTTC CACCTGCAGC GTCTTGGCAA AGCATCAGCA 
GCCTCCGGTG GCCGGCTCAA GTTCTCTGCA GCCTGCGGCC CCCCCGTGGC CCCTGAGTGC 
GAGCACTGTG GGCAGCGACA CCAGCTTGGT GGCCCCATGT GGGCAGAGCC CCTCCATGAC 
CTGGAATTCG TGGGCCGTGT CCTCGAGGCT GTGAGCGCCA ACCCTGGCCG CTTCCACACC 
GCAGAGCGGA TCCGAGGAGT GCTGAGTGTC ATCACGGAGG AGCTCCCGGA CGTGCCTCTC 
TACTATACGC TGGACCAGCT GAGCAGCACC ATCCACTGCA ACACACCCAG CCTCCTGCAG 
CTGCGGTCGG CCCTCCTCCA CGCTGGCTTC CGGGTCTCCC TCTCCCATGC CTGTAAGAAT 
GCTGTGAAGA CAGACGCTCC CTCCTCGACC CTCTGGGACA TCATGCGGTG CTGGGAGAAG 
GAGTGTCCAG TGAAACGGGA GCGCCTGTCA GAGAGCAGCC CAGCCTTCCG CATTCTCAGC 
GTGGAGCCCA GGCTGCAGGC CAACTTCACC ATCCGGGATG ATGCCAACCC CAGCTCCCGC 
CAGCGAGGAC TCAAGCGCTT CCAGGCCAAC CCTGAGGCCA ACTGGGGTCC CCGGCCCCGT 
GCCCGGCCAG GAGGCAAGGC AGCAGGAGAA ACTGTGGAAG AGAGACGTAG GCTGCTCCAA 
AATAAGCGAA AAGAGCCGGT GGAGGACCCA GCTGAGCGGG CTGCCTGGCT CAAAACATTT 
CCCTGCAAGA GGTTCAAGGA GGGCACCTGT CAACAGGGGG GCCAGTGCTG CTACTCGCAT 
AGCCCCCCCT CACCTAAGGC CACTGCTGAA GCCACCCCCA CTGACTGTCC AGAGGCCCCC 
AGTCAGAACC CCGCTGAGCC TGGGGCTGCT ACTGGTCCAG GCACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327437.1
CDS	17..2047
Translation	MICPPSVPTCRRPGPNPASVHRDQFDFRCLERRLYRARFMEGQPQGPPNSAAMENGTEPDGEERPPGFQETTITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKVVVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPYGSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASAASGGRLKFSAACGPPVAPECEHCGQRHQLGGPMWAEPLHDLEFVGRVLEAVSANPGRFHTAERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHAGFRVSLSHACKNAVKTDAPSSTLWDIMRCWEKECPVKRERLSESSPAFRILSVEPRLQANFTIRDDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAAGETVEERRRLLQNKRKEPVEDPAERAAWLKTFPCKRFKEGTCQQGGQCCYSHSPPSPKATAEATPTDCPEAPSQNPAEPGAATGPGTE

Target ORF information:

RefSeq Version	XM_015471951.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471951.1

ORF Insert Sequence:

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGTC TCTATAGAGC CCGGTTTATG 
GAGGGGCAGC CCCAAGGGCC GCCAAATTCA GCAGCCATGG AGAATGGCAC CGAGCCGGAC 
GGAGAAGAGC GCCCACCTGG GTTCCAGGAG ACGACGATCA CCGAGGGGGC CGCCAGGATC 
GTCTTTCCCA ACGCCAACGA AGTTTTCTAC AACCCAGTGC AAGAGTTCAA CCGGGACCTA 
ACATGCGCTG TGATCACGGA ATTTGCTCGT ATTCAGCTTG CGGCCAAAGG AATCCAGATC 
AAGGTGCCAG GTGAGAAGGA TGTGCAAAAG GTGGTCGTGG ACTTGTCAGA GCCAACGGAG 
GACAAGGCTG AACTGAAAGA GGGTGCAAAC CTGGCCCTGG AAGACCAGCC TCGAACAGCC 
GCCGTGGGGG AGATCTGTGA GGAAGGCCTG CGAGTGCTGG AAGGCCTGGC AGCCTCCGGC 
TTACGTTCCA TTCGCTTTGC TCGAGAGGTG CCTGGTCTCC GATCCGTGGT TGCCAATGAC 
GCCTCTGCCC GAGCTGTGGA TCTTATGCGC CGTAATGTGC AGCTCAACGA AGTGGCCCAC 
CTGGTACAGC CCAGCCAGGC AGATGCCCGG ATGCTGATGT ACCAGCACCA GAAGGCGTCG 
GAGAGGTTTG ACGTCATCGA CCTCGACCCT TACGGCAGCC CCGCCTCTTT CCTGGATGCA 
GCCGTGCAGG CTGTGAGTGA AGGAGGGCTG CTGTGCGTCA CCTGCACAGA TATGGCGGTC 
CTGGCGGGGA ACAGCGGGGA GACCTGCTAC AGCAAATACG GGGCCATGGC CCTCAAGAGC 
CGGGCCTGCC ACGAGATGGC CCTGAGGACT GTCCTGCACA GCCTGGATCT CCGTGCCAAC 
TGCTACCAGC GCTTTGTGGT GCCACTGCTC AGCATCAGCG CTGACTTCTA TGTACGGGTT 
TTTGTTCGTG TCTTCACTGG TCAGGCCAAG GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG 
GTGTTCCAGT GCGTGGGCTG CGGGGCCTTC CACCTGCAGC GTCTTGGCAA AGCATCAGCA 
GCCTCCGGTG GCCGGCTCAA GTTCTCTGCA GCCTGCGGCC CCCCCGTGGC CCCTGAGTGC 
GAGCACTGTG GGCAGCGACA CCAGCTTGGT GGCCCCATGT GGGCAGAGCC CCTCCATGAC 
CTGGAATTCG TGGGCCGTGT CCTCGAGGCT GTGAGCGCCA ACCCTGGCCG CTTCCACACC 
GCAGAGCGGA TCCGAGGAGT GCTGAGTGTC ATCACGGAGG AGCTCCCGGA CGTGCCTCTC 
TACTATACGC TGGACCAGCT GAGCAGCACC ATCCACTGCA ACACACCCAG CCTCCTGCAG 
CTGCGGTCGG CCCTCCTCCA CGCTGGCTTC CGGGTCTCCC TCTCCCATGC CTGTAAGAAT 
GCTGTGAAGA CAGACGCTCC CTCCTCGACC CTCTGGGACA TCATGCGGTG CTGGGAGAAG 
GAGTGTCCAG TGAAACGGGA GCGCCTGTCA GAGAGCAGCC CAGCCTTCCG CATTCTCAGC 
GTGGAGCCCA GGCTGCAGGC CAACTTCACC ATCCGGGATG ATGCCAACCC CAGCTCCCGC 
CAGCGAGGAC TCAAGCGCTT CCAGGCCAAC CCTGAGGCCA ACTGGGGTCC CCGGCCCCGT 
GCCCGGCCAG GAGGCAAGGC AGCAGGAGAA ACTGTGGAAG AGAGACGTAG GCTGCTCCAA 
AATAAGCGAA AAGAGCCGGT GGAGGACCCA GCTGAGCGGG CTGCCTGGCT CAAAACATTT 
CCCTGCAAGA GGTTCAAGGA GGGCACCTGT CAACAGGGGG GCCAGTGCTG CTACTCGCAT 
AGCCCCCCCT CACCTAAGGC CACTGCTGAA GCCACCCCCA CTGACTGTCC AGAGGCCCCC 
AGTCAGAACC CCGCTGAGCC TGGGGCTGCT ACTGGTCCAG GCACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_015471951.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327437.1 Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20923
Clone ID Related Accession (Same CDS sequence)	XM_015471951.1 , XM_015471951.2
Accession Version	XM_015471951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGTC TCTATAGAGC CCGGTTTATG 
GAGGGGCAGC CCCAAGGGCC GCCAAATTCA GCAGCCATGG AGAATGGCAC CGAGCCGGAC 
GGAGAAGAGC GCCCACCTGG GTTCCAGGAG ACGACGATCA CCGAGGGGGC CGCCAGGATC 
GTCTTTCCCA ACGCCAACGA AGTTTTCTAC AACCCAGTGC AAGAGTTCAA CCGGGACCTA 
ACATGCGCTG TGATCACGGA ATTTGCTCGT ATTCAGCTTG CGGCCAAAGG AATCCAGATC 
AAGGTGCCAG GTGAGAAGGA TGTGCAAAAG GTGGTCGTGG ACTTGTCAGA GCCAACGGAG 
GACAAGGCTG AACTGAAAGA GGGTGCAAAC CTGGCCCTGG AAGACCAGCC TCGAACAGCC 
GCCGTGGGGG AGATCTGTGA GGAAGGCCTG CGAGTGCTGG AAGGCCTGGC AGCCTCCGGC 
TTACGTTCCA TTCGCTTTGC TCGAGAGGTG CCTGGTCTCC GATCCGTGGT TGCCAATGAC 
GCCTCTGCCC GAGCTGTGGA TCTTATGCGC CGTAATGTGC AGCTCAACGA AGTGGCCCAC 
CTGGTACAGC CCAGCCAGGC AGATGCCCGG ATGCTGATGT ACCAGCACCA GAAGGCGTCG 
GAGAGGTTTG ACGTCATCGA CCTCGACCCT TACGGCAGCC CCGCCTCTTT CCTGGATGCA 
GCCGTGCAGG CTGTGAGTGA AGGAGGGCTG CTGTGCGTCA CCTGCACAGA TATGGCGGTC 
CTGGCGGGGA ACAGCGGGGA GACCTGCTAC AGCAAATACG GGGCCATGGC CCTCAAGAGC 
CGGGCCTGCC ACGAGATGGC CCTGAGGACT GTCCTGCACA GCCTGGATCT CCGTGCCAAC 
TGCTACCAGC GCTTTGTGGT GCCACTGCTC AGCATCAGCG CTGACTTCTA TGTACGGGTT 
TTTGTTCGTG TCTTCACTGG TCAGGCCAAG GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG 
GTGTTCCAGT GCGTGGGCTG CGGGGCCTTC CACCTGCAGC GTCTTGGCAA AGCATCAGCA 
GCCTCCGGTG GCCGGCTCAA GTTCTCTGCA GCCTGCGGCC CCCCCGTGGC CCCTGAGTGC 
GAGCACTGTG GGCAGCGACA CCAGCTTGGT GGCCCCATGT GGGCAGAGCC CCTCCATGAC 
CTGGAATTCG TGGGCCGTGT CCTCGAGGCT GTGAGCGCCA ACCCTGGCCG CTTCCACACC 
GCAGAGCGGA TCCGAGGAGT GCTGAGTGTC ATCACGGAGG AGCTCCCGGA CGTGCCTCTC 
TACTATACGC TGGACCAGCT GAGCAGCACC ATCCACTGCA ACACACCCAG CCTCCTGCAG 
CTGCGGTCGG CCCTCCTCCA CGCTGGCTTC CGGGTCTCCC TCTCCCATGC CTGTAAGAAT 
GCTGTGAAGA CAGACGCTCC CTCCTCGACC CTCTGGGACA TCATGCGGTG CTGGGAGAAG 
GAGTGTCCAG TGAAACGGGA GCGCCTGTCA GAGAGCAGCC CAGCCTTCCG CATTCTCAGC 
GTGGAGCCCA GGCTGCAGGC CAACTTCACC ATCCGGGATG ATGCCAACCC CAGCTCCCGC 
CAGCGAGGAC TCAAGCGCTT CCAGGCCAAC CCTGAGGCCA ACTGGGGTCC CCGGCCCCGT 
GCCCGGCCAG GAGGCAAGGC AGCAGGAGAA ACTGTGGAAG AGAGACGTAG GCTGCTCCAA 
AATAAGCGAA AAGAGCCGGT GGAGGACCCA GCTGAGCGGG CTGCCTGGCT CAAAACATTT 
CCCTGCAAGA GGTTCAAGGA GGGCACCTGT CAACAGGGGG GCCAGTGCTG CTACTCGCAT 
AGCCCCCCCT CACCTAAGGC CACTGCTGAA GCCACCCCCA CTGACTGTCC AGAGGCCCCC 
AGTCAGAACC CCGCTGAGCC TGGGGCTGCT ACTGGTCCAG GCACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327437.1
CDS	412..2442
Translation	MICPPSVPTCRRPGPNPASVHRDQFDFRCLERRLYRARFMEGQPQGPPNSAAMENGTEPDGEERPPGFQETTITEGAARIVFPNANEVFYNPVQEFNRDLTCAVITEFARIQLAAKGIQIKVPGEKDVQKVVVDLSEPTEDKAELKEGANLALEDQPRTAAVGEICEEGLRVLEGLAASGLRSIRFAREVPGLRSVVANDASARAVDLMRRNVQLNEVAHLVQPSQADARMLMYQHQKASERFDVIDLDPYGSPASFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRTVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASAASGGRLKFSAACGPPVAPECEHCGQRHQLGGPMWAEPLHDLEFVGRVLEAVSANPGRFHTAERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHAGFRVSLSHACKNAVKTDAPSSTLWDIMRCWEKECPVKRERLSESSPAFRILSVEPRLQANFTIRDDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAAGETVEERRRLLQNKRKEPVEDPAERAAWLKTFPCKRFKEGTCQQGGQCCYSHSPPSPKATAEATPTDCPEAPSQNPAEPGAATGPGTE

Target ORF information:

RefSeq Version	XM_015471951.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471951.2

ORF Insert Sequence:

ATGATCTGCC CGCCCTCTGT GCCAACATGT CGGCGCCCAG GTCCTAATCC GGCAAGCGTG 
CACCGAGACC AGTTCGATTT TCGGTGTCTC GAGAGACGTC TCTATAGAGC CCGGTTTATG 
GAGGGGCAGC CCCAAGGGCC GCCAAATTCA GCAGCCATGG AGAATGGCAC CGAGCCGGAC 
GGAGAAGAGC GCCCACCTGG GTTCCAGGAG ACGACGATCA CCGAGGGGGC CGCCAGGATC 
GTCTTTCCCA ACGCCAACGA AGTTTTCTAC AACCCAGTGC AAGAGTTCAA CCGGGACCTA 
ACATGCGCTG TGATCACGGA ATTTGCTCGT ATTCAGCTTG CGGCCAAAGG AATCCAGATC 
AAGGTGCCAG GTGAGAAGGA TGTGCAAAAG GTGGTCGTGG ACTTGTCAGA GCCAACGGAG 
GACAAGGCTG AACTGAAAGA GGGTGCAAAC CTGGCCCTGG AAGACCAGCC TCGAACAGCC 
GCCGTGGGGG AGATCTGTGA GGAAGGCCTG CGAGTGCTGG AAGGCCTGGC AGCCTCCGGC 
TTACGTTCCA TTCGCTTTGC TCGAGAGGTG CCTGGTCTCC GATCCGTGGT TGCCAATGAC 
GCCTCTGCCC GAGCTGTGGA TCTTATGCGC CGTAATGTGC AGCTCAACGA AGTGGCCCAC 
CTGGTACAGC CCAGCCAGGC AGATGCCCGG ATGCTGATGT ACCAGCACCA GAAGGCGTCG 
GAGAGGTTTG ACGTCATCGA CCTCGACCCT TACGGCAGCC CCGCCTCTTT CCTGGATGCA 
GCCGTGCAGG CTGTGAGTGA AGGAGGGCTG CTGTGCGTCA CCTGCACAGA TATGGCGGTC 
CTGGCGGGGA ACAGCGGGGA GACCTGCTAC AGCAAATACG GGGCCATGGC CCTCAAGAGC 
CGGGCCTGCC ACGAGATGGC CCTGAGGACT GTCCTGCACA GCCTGGATCT CCGTGCCAAC 
TGCTACCAGC GCTTTGTGGT GCCACTGCTC AGCATCAGCG CTGACTTCTA TGTACGGGTT 
TTTGTTCGTG TCTTCACTGG TCAGGCCAAG GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG 
GTGTTCCAGT GCGTGGGCTG CGGGGCCTTC CACCTGCAGC GTCTTGGCAA AGCATCAGCA 
GCCTCCGGTG GCCGGCTCAA GTTCTCTGCA GCCTGCGGCC CCCCCGTGGC CCCTGAGTGC 
GAGCACTGTG GGCAGCGACA CCAGCTTGGT GGCCCCATGT GGGCAGAGCC CCTCCATGAC 
CTGGAATTCG TGGGCCGTGT CCTCGAGGCT GTGAGCGCCA ACCCTGGCCG CTTCCACACC 
GCAGAGCGGA TCCGAGGAGT GCTGAGTGTC ATCACGGAGG AGCTCCCGGA CGTGCCTCTC 
TACTATACGC TGGACCAGCT GAGCAGCACC ATCCACTGCA ACACACCCAG CCTCCTGCAG 
CTGCGGTCGG CCCTCCTCCA CGCTGGCTTC CGGGTCTCCC TCTCCCATGC CTGTAAGAAT 
GCTGTGAAGA CAGACGCTCC CTCCTCGACC CTCTGGGACA TCATGCGGTG CTGGGAGAAG 
GAGTGTCCAG TGAAACGGGA GCGCCTGTCA GAGAGCAGCC CAGCCTTCCG CATTCTCAGC 
GTGGAGCCCA GGCTGCAGGC CAACTTCACC ATCCGGGATG ATGCCAACCC CAGCTCCCGC 
CAGCGAGGAC TCAAGCGCTT CCAGGCCAAC CCTGAGGCCA ACTGGGGTCC CCGGCCCCGT 
GCCCGGCCAG GAGGCAAGGC AGCAGGAGAA ACTGTGGAAG AGAGACGTAG GCTGCTCCAA 
AATAAGCGAA AAGAGCCGGT GGAGGACCCA GCTGAGCGGG CTGCCTGGCT CAAAACATTT 
CCCTGCAAGA GGTTCAAGGA GGGCACCTGT CAACAGGGGG GCCAGTGCTG CTACTCGCAT 
AGCCCCCCCT CACCTAAGGC CACTGCTGAA GCCACCCCCA CTGACTGTCC AGAGGCCCCC 
AGTCAGAACC CCGCTGAGCC TGGGGCTGCT ACTGGTCCAG GCACAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TRMT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム