GIGYF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010819577.2	XP_010817879.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X4
XM_015460477.1	XP_015315963.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460476.2	XP_015315962.1	GRB10-interacting GYF protein 1 isoform X1
XM_015460480.2	XP_015315966.1	GRB10-interacting GYF protein 1 isoform X1
XM_015460481.1	XP_015315967.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X2
XM_024984969.1	XP_024840737.1	GRB10-interacting GYF protein 1 isoform X3
XM_024984970.1	XP_024840738.1	GRB10-interacting GYF protein 1 isoform X4
XM_024984971.1	XP_024840739.1	GRB10-interacting GYF protein 1 isoform X5
XM_015460474.1	XP_015315960.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460476.1	XP_015315962.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_010819566.2	XP_010817868.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X3
XM_015460477.2	XP_015315963.1	GRB10-interacting GYF protein 1 isoform X1
NM_001205998.1	NP_001192927.1	GRB10-interacting GYF protein 1
XM_015460473.1	XP_015315959.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460478.1	XP_015315964.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460479.1	XP_015315965.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460480.1	XP_015315966.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460475.2	XP_015315961.1	GRB10-interacting GYF protein 1 isoform X1
XM_024984967.1	XP_024840735.1	GRB10-interacting GYF protein 1 isoform X2
XM_015460475.1	XP_015315961.1	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
XM_015460478.2	XP_015315964.1	GRB10-interacting GYF protein 1 isoform X1
XM_024984968.1	XP_024840736.1	GRB10-interacting GYF protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230328	XM_024984971.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa33276	NM_001205998.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33274	XM_024984967.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33274	XM_010819566.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33275	XM_024984968.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33275	XM_024984969.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33275	XM_010819577.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa33277	XM_024984970.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460478.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460477.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460479.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460480.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460473.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460474.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460475.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460476.2 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88575	XM_015460481.1 Latest version!	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBa88574	XM_015460476.1	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460477.1	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460480.1	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460475.1	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa88574	XM_015460478.1	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

GIGYF1 ( XM_024984971.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840739.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa230328
Clone ID Related Accession (Same CDS sequence)	XM_024984971.1
Accession Version	XM_024984971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG 
CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCC GCCAGCTCCC GCAGTGCGTG 
CTCCGGGAAA AGGCAGCTCT GGGAGACCTG CCGCCGCCGC AGCAGCAGCA GCTCACCGCG 
TTCCTGCAGC AGCTCCAAGC TCTCAAACCC CCCAGGGGCG GGGACCAGAA CTTGCTCCCG 
ACGATGAACC GGTCCTTGTC GGTGCCAGAC TCGGGTCCCC TCTGGGACAT ACATACCTCA 
GCCTCACCAC AGTCAGGCGG TGAGGCCAGT CTTTGGGATA TACCAATTAA CTCTTCGACT 
CAGGGTCCAA TTCTAGAACA ACTCCAGCTG CAACACAAAT TCCAGGAGCG CAGAGAAGTG 
GAGCTCAGGG CGAAGCGGGA GGAGGAGGAG CGGAAGCGCC GGGAAGAGAA GCGCCGCCAG 
CAGCAGCAGC AGCAGGAGGA GCAGAAGCGG CGGCAGGAGG AAGAGGAGCT GTTCCGGCGC 
AAGCAGGCGC GGCAGCAGGA GCTGCTGCTG AAGCTGCTGC AGCAGCAGCA GCAGGCGGTG 
GCCGCCGTCC CCGCGCCTCC TACACCCAGT TCCCCGCCTC CGCTGTGGGC TGGCCTGGCC 
AAGCAGGGCC TGTCCATGAA GACGCTGCTG GAGCTGCAGC TGGAGGGCGA GCGGCACCTG 
CATAAGCAGC CCCCGCCTCG GGAGCCATCG CGGGCCCAGG CCCCCAACCA CCGCGTGCAG 
CTCGGGGGCC TGGGCTCCGC CCCCCTGAAC CAGTGGGTAT CTGAGGCCGG GCCGCTGTGG 
GGTGGGCCCG ACAAGAGTGG GGGCGGCAGC AGCAGCCTGG GCCTCTGGGA AGACACCCTC 
AAGAGCAGTG GGAGCCTGGC CCGGAGCCTG GGCCTGAAGA ACAGCCGCAG CAGCCCCTCT 
CTCAGTGACT CGTACAGCCA CTTGTCTGGT CGGCCTGTGC GCAAAAAGAC GGAGGAGGAA 
GAGAAGCTGC TGAAGCTGCT GCAGGGCATC CCCCGGCCCC AGGATGGCTT CACCCAGTGG 
TGTGAGCAGA TGCTGCACAC GCTGAGCACC ACGGGCAGCC TGGACGTGCC CATGGCTGTA 
GCGATCCTCA AGGAGGTGGA ATCCCCCTAT GACGTCCACG ACTATATTCG TTCCTGCCTG 
GGGGACACGC TGGAAGCCAA AGAATTTGCC AAACAATTCC TGGAGCGGAG GGCCAAGCAG 
AAGGCCAGCC AGCAGCGGCA GCAGCAGCAG GAGGCTTGGC TGAGCAGCGG CTCCCTACAG 
ACAGCCTTTC AGACCAACCA CAGCACCAAG CTTGGCCCCG GGGAGGGCAG CAAGGCCAAG 
AGGCGGGCAC TGATGCTGCA CTCGGACCCC AGCATCTTGG GGTACTCCCT GCACGGACCT 
TCTGGTGAGA TCGAGAGCGT GGATGACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840739.1
CDS	661..3753
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_024984971.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984971.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG 
CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCC GCCAGCTCCC GCAGTGCGTG 
CTCCGGGAAA AGGCAGCTCT GGGAGACCTG CCGCCGCCGC AGCAGCAGCA GCTCACCGCG 
TTCCTGCAGC AGCTCCAAGC TCTCAAACCC CCCAGGGGCG GGGACCAGAA CTTGCTCCCG 
ACGATGAACC GGTCCTTGTC GGTGCCAGAC TCGGGTCCCC TCTGGGACAT ACATACCTCA 
GCCTCACCAC AGTCAGGCGG TGAGGCCAGT CTTTGGGATA TACCAATTAA CTCTTCGACT 
CAGGGTCCAA TTCTAGAACA ACTCCAGCTG CAACACAAAT TCCAGGAGCG CAGAGAAGTG 
GAGCTCAGGG CGAAGCGGGA GGAGGAGGAG CGGAAGCGCC GGGAAGAGAA GCGCCGCCAG 
CAGCAGCAGC AGCAGGAGGA GCAGAAGCGG CGGCAGGAGG AAGAGGAGCT GTTCCGGCGC 
AAGCAGGCGC GGCAGCAGGA GCTGCTGCTG AAGCTGCTGC AGCAGCAGCA GCAGGCGGTG 
GCCGCCGTCC CCGCGCCTCC TACACCCAGT TCCCCGCCTC CGCTGTGGGC TGGCCTGGCC 
AAGCAGGGCC TGTCCATGAA GACGCTGCTG GAGCTGCAGC TGGAGGGCGA GCGGCACCTG 
CATAAGCAGC CCCCGCCTCG GGAGCCATCG CGGGCCCAGG CCCCCAACCA CCGCGTGCAG 
CTCGGGGGCC TGGGCTCCGC CCCCCTGAAC CAGTGGGTAT CTGAGGCCGG GCCGCTGTGG 
GGTGGGCCCG ACAAGAGTGG GGGCGGCAGC AGCAGCCTGG GCCTCTGGGA AGACACCCTC 
AAGAGCAGTG GGAGCCTGGC CCGGAGCCTG GGCCTGAAGA ACAGCCGCAG CAGCCCCTCT 
CTCAGTGACT CGTACAGCCA CTTGTCTGGT CGGCCTGTGC GCAAAAAGAC GGAGGAGGAA 
GAGAAGCTGC TGAAGCTGCT GCAGGGCATC CCCCGGCCCC AGGATGGCTT CACCCAGTGG 
TGTGAGCAGA TGCTGCACAC GCTGAGCACC ACGGGCAGCC TGGACGTGCC CATGGCTGTA 
GCGATCCTCA AGGAGGTGGA ATCCCCCTAT GACGTCCACG ACTATATTCG TTCCTGCCTG 
GGGGACACGC TGGAAGCCAA AGAATTTGCC AAACAATTCC TGGAGCGGAG GGCCAAGCAG 
AAGGCCAGCC AGCAGCGGCA GCAGCAGCAG GAGGCTTGGC TGAGCAGCGG CTCCCTACAG 
ACAGCCTTTC AGACCAACCA CAGCACCAAG CTTGGCCCCG GGGAGGGCAG CAAGGCCAAG 
AGGCGGGCAC TGATGCTGCA CTCGGACCCC AGCATCTTGG GGTACTCCCT GCACGGACCT 
TCTGGTGAGA TCGAGAGCGT GGATGACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( NM_001205998.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192927.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), mRNA.

Price & Availability↑

CloneID	OBa33276
Clone ID Related Accession (Same CDS sequence)	NM_001205998.1
Accession Version	NM_001205998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Apr 20, 2011 this sequence version replaced XM_610923.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGAGGAC 
ATGAGGGGAA TGCAGCTAAG TCCTGGGGCG GGCTCACCGC CTGGCCCACC AGACCTGGAA 
GATGATGAAG GCTTGAAGCA CCTGCAGCAG GAGGCGGAGA AGCTCGTGGC CTCCCTGCAG 
GACAGCTCCC TGGAGGAGGA GCAGTTCACG GCCGCCATCC AGGCCCAGGG CCTGCGTCAC 
TCTGCAGCCG CCACTGCCCT CCCCCTCAGC CATGGTGCGG CCCGGAAGTG GTTCTACAAG 
GACCCGCAGG GCGAGATCCA AGGCCCCTTC ACGACCCAGG AGATGGCGGA GTGGTTCCAG 
GCGGGCTACT TCTCCATGGC CCTGCTGGTG AAGCGGGGCT GTGATGAGGG CTTCCAGCCA 
CTGGGTGAGG TGATCAAGAT GTGGGGTCGC GTACCCTTTG CCCCTGGGCC CTCACCTCCC 
CCGCTGCTGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC 
CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG 
CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG 
AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC 
ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT 
AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG 
CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG 
AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG 
CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG 
CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG 
GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC 
GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC 
CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC 
GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG 
GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC 
AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG 
ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC 
TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG 
CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT 
CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG 
AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC 
GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC 
AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC 
CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192927.1
CDS	1..3105
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	NM_001205998.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205998.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGAGGAC 
ATGAGGGGAA TGCAGCTAAG TCCTGGGGCG GGCTCACCGC CTGGCCCACC AGACCTGGAA 
GATGATGAAG GCTTGAAGCA CCTGCAGCAG GAGGCGGAGA AGCTCGTGGC CTCCCTGCAG 
GACAGCTCCC TGGAGGAGGA GCAGTTCACG GCCGCCATCC AGGCCCAGGG CCTGCGTCAC 
TCTGCAGCCG CCACTGCCCT CCCCCTCAGC CATGGTGCGG CCCGGAAGTG GTTCTACAAG 
GACCCGCAGG GCGAGATCCA AGGCCCCTTC ACGACCCAGG AGATGGCGGA GTGGTTCCAG 
GCGGGCTACT TCTCCATGGC CCTGCTGGTG AAGCGGGGCT GTGATGAGGG CTTCCAGCCA 
CTGGGTGAGG TGATCAAGAT GTGGGGTCGC GTACCCTTTG CCCCTGGGCC CTCACCTCCC 
CCGCTGCTGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC 
CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG 
CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG 
AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC 
ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT 
AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG 
CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG 
AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG 
CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG 
CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG 
GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC 
GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC 
CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC 
GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG 
GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC 
AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG 
ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC 
TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG 
CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT 
CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG 
AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC 
GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC 
AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC 
CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_010819566.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817868.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

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CloneID	OBa33274
Clone ID Related Accession (Same CDS sequence)	XM_010819566.2 , XM_024984967.1
Accession Version	XM_010819566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC 
CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG 
CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG 
GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC 
TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA 
CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC 
CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG 
GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA 
GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG 
CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG 
CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG 
GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC 
CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT 
GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC 
CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC 
AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC 
AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG 
GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG 
GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC 
TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG 
GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG 
AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG 
GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG 
TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817868.1
CDS	1690..4800
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_010819566.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819566.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC 
CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG 
CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG 
GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC 
TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA 
CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC 
CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG 
GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA 
GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG 
CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG 
CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG 
GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC 
CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT 
GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC 
CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC 
AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC 
AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG 
GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG 
GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC 
TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG 
GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG 
AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG 
GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG 
TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_024984967.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840735.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa33274
Clone ID Related Accession (Same CDS sequence)	XM_010819566.2 , XM_024984967.1
Accession Version	XM_024984967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC 
CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG 
CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG 
GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC 
TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA 
CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC 
CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG 
GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA 
GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG 
CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG 
CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG 
GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC 
CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT 
GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC 
CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC 
AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC 
AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG 
GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG 
GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC 
TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG 
GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG 
AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG 
GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG 
TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840735.1
CDS	661..3771
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_024984967.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984967.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC 
CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG 
CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG 
GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC 
TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA 
CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC 
CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG 
GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA 
GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG 
CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG 
CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG 
GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC 
CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT 
GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC 
CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC 
AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC 
AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG 
GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG 
GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC 
TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG 
GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG 
AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG 
GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG 
TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_010819577.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817879.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X11, mRNA.

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CloneID	OBa33275
Clone ID Related Accession (Same CDS sequence)	XM_010819577.2 , XM_024984969.1 , XM_024984968.1
Accession Version	XM_010819577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817879.1
CDS	1690..4797
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_010819577.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819577.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_024984969.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840737.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa33275
Clone ID Related Accession (Same CDS sequence)	XM_010819577.2 , XM_024984969.1 , XM_024984968.1
Accession Version	XM_024984969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840737.1
CDS	544..3651
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_024984969.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984969.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_024984968.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840736.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa33275
Clone ID Related Accession (Same CDS sequence)	XM_010819577.2 , XM_024984969.1 , XM_024984968.1
Accession Version	XM_024984968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840736.1
CDS	661..3768
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_024984968.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984968.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA 
GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_024984970.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840738.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa33277
Clone ID Related Accession (Same CDS sequence)	XM_024984970.1
Accession Version	XM_024984970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG 
CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCA GCCGCCAGCT CCCGCAGTGC 
GTGCTCCGGG AAAAGGCAGC TCTGGGAGAC CTGCCGCCGC CGCAGCAGCA GCAGCTCACC 
GCGTTCCTGC AGCAGCTCCA AGCTCTCAAA CCCCCCAGGG GCGGGGACCA GAACTTGCTC 
CCGACGATGA ACCGGTCCTT GTCGGTGCCA GACTCGGGTC CCCTCTGGGA CATACATACC 
TCAGCCTCAC CACAGTCAGG CGGTGAGGCC AGTCTTTGGG ATATACCAAT TAACTCTTCG 
ACTCAGGGTC CAATTCTAGA ACAACTCCAG CTGCAACACA AATTCCAGGA GCGCAGAGAA 
GTGGAGCTCA GGGCGAAGCG GGAGGAGGAG GAGCGGAAGC GCCGGGAAGA GAAGCGCCGC 
CAGCAGCAGC AGCAGCAGGA GGAGCAGAAG CGGCGGCAGG AGGAAGAGGA GCTGTTCCGG 
CGCAAGCAGG CGCGGCAGCA GGAGCTGCTG CTGAAGCTGC TGCAGCAGCA GCAGCAGGCG 
GTGGCCGCCG TCCCCGCGCC TCCTACACCC AGTTCCCCGC CTCCGCTGTG GGCTGGCCTG 
GCCAAGCAGG GCCTGTCCAT GAAGACGCTG CTGGAGCTGC AGCTGGAGGG CGAGCGGCAC 
CTGCATAAGC AGCCCCCGCC TCGGGAGCCA TCGCGGGCCC AGGCCCCCAA CCACCGCGTG 
CAGCTCGGGG GCCTGGGCTC CGCCCCCCTG AACCAGTGGG TATCTGAGGC CGGGCCGCTG 
TGGGGTGGGC CCGACAAGAG TGGGGGCGGC AGCAGCAGCC TGGGCCTCTG GGAAGACACC 
CTCAAGAGCA GTGGGAGCCT GGCCCGGAGC CTGGGCCTGA AGAACAGCCG CAGCAGCCCC 
TCTCTCAGTG ACTCGTACAG CCACTTGTCT GGTCGGCCTG TGCGCAAAAA GACGGAGGAG 
GAAGAGAAGC TGCTGAAGCT GCTGCAGGGC ATCCCCCGGC CCCAGGATGG CTTCACCCAG 
TGGTGTGAGC AGATGCTGCA CACGCTGAGC ACCACGGGCA GCCTGGACGT GCCCATGGCT 
GTAGCGATCC TCAAGGAGGT GGAATCCCCC TATGACGTCC ACGACTATAT TCGTTCCTGC 
CTGGGGGACA CGCTGGAAGC CAAAGAATTT GCCAAACAAT TCCTGGAGCG GAGGGCCAAG 
CAGAAGGCCA GCCAGCAGCG GCAGCAGCAG CAGGAGGCTT GGCTGAGCAG CGGCTCCCTA 
CAGACAGCCT TTCAGACCAA CCACAGCACC AAGCTTGGCC CCGGGGAGGG CAGCAAGGCC 
AAGAGGCGGG CACTGATGCT GCACTCGGAC CCCAGCATCT TGGGGTACTC CCTGCACGGA 
CCTTCTGGTG AGATCGAGAG CGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840738.1
CDS	661..3756
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_024984970.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984970.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG 
CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCA GCCGCCAGCT CCCGCAGTGC 
GTGCTCCGGG AAAAGGCAGC TCTGGGAGAC CTGCCGCCGC CGCAGCAGCA GCAGCTCACC 
GCGTTCCTGC AGCAGCTCCA AGCTCTCAAA CCCCCCAGGG GCGGGGACCA GAACTTGCTC 
CCGACGATGA ACCGGTCCTT GTCGGTGCCA GACTCGGGTC CCCTCTGGGA CATACATACC 
TCAGCCTCAC CACAGTCAGG CGGTGAGGCC AGTCTTTGGG ATATACCAAT TAACTCTTCG 
ACTCAGGGTC CAATTCTAGA ACAACTCCAG CTGCAACACA AATTCCAGGA GCGCAGAGAA 
GTGGAGCTCA GGGCGAAGCG GGAGGAGGAG GAGCGGAAGC GCCGGGAAGA GAAGCGCCGC 
CAGCAGCAGC AGCAGCAGGA GGAGCAGAAG CGGCGGCAGG AGGAAGAGGA GCTGTTCCGG 
CGCAAGCAGG CGCGGCAGCA GGAGCTGCTG CTGAAGCTGC TGCAGCAGCA GCAGCAGGCG 
GTGGCCGCCG TCCCCGCGCC TCCTACACCC AGTTCCCCGC CTCCGCTGTG GGCTGGCCTG 
GCCAAGCAGG GCCTGTCCAT GAAGACGCTG CTGGAGCTGC AGCTGGAGGG CGAGCGGCAC 
CTGCATAAGC AGCCCCCGCC TCGGGAGCCA TCGCGGGCCC AGGCCCCCAA CCACCGCGTG 
CAGCTCGGGG GCCTGGGCTC CGCCCCCCTG AACCAGTGGG TATCTGAGGC CGGGCCGCTG 
TGGGGTGGGC CCGACAAGAG TGGGGGCGGC AGCAGCAGCC TGGGCCTCTG GGAAGACACC 
CTCAAGAGCA GTGGGAGCCT GGCCCGGAGC CTGGGCCTGA AGAACAGCCG CAGCAGCCCC 
TCTCTCAGTG ACTCGTACAG CCACTTGTCT GGTCGGCCTG TGCGCAAAAA GACGGAGGAG 
GAAGAGAAGC TGCTGAAGCT GCTGCAGGGC ATCCCCCGGC CCCAGGATGG CTTCACCCAG 
TGGTGTGAGC AGATGCTGCA CACGCTGAGC ACCACGGGCA GCCTGGACGT GCCCATGGCT 
GTAGCGATCC TCAAGGAGGT GGAATCCCCC TATGACGTCC ACGACTATAT TCGTTCCTGC 
CTGGGGGACA CGCTGGAAGC CAAAGAATTT GCCAAACAAT TCCTGGAGCG GAGGGCCAAG 
CAGAAGGCCA GCCAGCAGCG GCAGCAGCAG CAGGAGGCTT GGCTGAGCAG CGGCTCCCTA 
CAGACAGCCT TTCAGACCAA CCACAGCACC AAGCTTGGCC CCGGGGAGGG CAGCAAGGCC 
AAGAGGCGGG CACTGATGCT GCACTCGGAC CCCAGCATCT TGGGGTACTC CCTGCACGGA 
CCTTCTGGTG AGATCGAGAG CGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460477.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315963.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460477.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315963.1
CDS	1507..4734
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460477.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460477.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460476.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315962.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315962.1
CDS	1426..4653
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460476.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460476.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460480.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315966.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315966.1
CDS	351..3578
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460480.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460480.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460474.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315960.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

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CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315960.1
CDS	994..4221
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460474.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460474.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460476.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315962.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315962.1
CDS	1441..4668
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460476.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460476.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460477.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315963.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315963.1
CDS	1492..4719
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460477.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460477.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460473.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315959.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315959.1
CDS	1489..4716
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460473.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460473.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460478.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315964.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315964.1
CDS	471..3698
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460478.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460478.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460479.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315965.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315965.1
CDS	680..3907
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460479.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460479.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460480.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315966.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315966.1
CDS	336..3563
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460480.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460480.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460475.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315961.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

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CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315961.1
CDS	1506..4733
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460475.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460475.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460475.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315961.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315961.1
CDS	1521..4748
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460475.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460475.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460478.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315964.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88574
Clone ID Related Accession (Same CDS sequence)	XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
Accession Version	XM_015460478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315964.1
CDS	486..3713
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460478.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460478.2

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG 
CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG 
CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC 
CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG 
GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA 
ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG 
GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA 
GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG 
GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG 
CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG 
TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG 
GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC 
AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG 
GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC 
TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC 
CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA 
AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT 
GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC 
GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT 
ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG 
CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC 
AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG 
GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC 
TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF1 ( XM_015460481.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315967.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

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CloneID	OBa88575
Clone ID Related Accession (Same CDS sequence)	XM_015460481.1
Accession Version	XM_015460481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PERQ amino acid-rich with GYF domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC 
CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG 
CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG 
AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC 
ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT 
AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG 
CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG 
AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG 
CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG 
CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG 
GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC 
GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC 
CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC 
GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG 
GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC 
AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG 
ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC 
TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG 
CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT 
CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG 
AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC 
GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC 
AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC 
CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315967.1
CDS	1690..4914
Translation	MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKESKVPDELQDKEFAALLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLGGASRGRGSTRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKSARRDGARSGFDEGGAGPRKEHARSDSENWRSLREEQEEEEEGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWREHGDRRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRGPDGFDDDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEEPSEGLDEEGPEAGGKELTPLPPQEEKSSSPSPLPTLGPLWGANGEGDDPVEKDLPAAEGEDMRGMQLSPGAGSPPGPPDLEDDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAIQAQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPFTTQEMAEWFQAGYFSMALLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLVSVPPLAWNREGRQEPGRGQRWVQSQTGAKPRERCGLTQGNMDQERLKKQQELAAAALYQQLQHQQFLQLVGSRQLPQCVLREKAALGDLPPPQQQQLTAFLQQLQALKPPRGGDQNLLPTMNRSLSVPDSGPLWDIHTSASPQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQEEEELFRRKQARQQELLLKLLQQQQQAVAAVPAPPTPSSPPPLWAGLAKQGLSMKTLLELQLEGERHLHKQPPPREPSRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGGSSSLGLWEDTLKSSGSLARSLGLKNSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQMLHTLSTTGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLSSGSLQTAFQTNHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGPSGEIESVDDY

Target ORF information:

RefSeq Version	XM_015460481.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460481.1

ORF Insert Sequence:

ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC 
TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG 
CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG 
CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG 
AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC 
ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA 
GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC 
AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT 
GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT 
CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG 
CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG 
CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG 
TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG 
CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC 
GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG 
GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA 
CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG 
CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG 
GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG 
GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG 
GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG 
CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT 
CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC 
AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC 
CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG 
CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT 
CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT 
GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC 
CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC 
GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC 
CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG 
CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG 
AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC 
ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT 
AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG 
CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG 
AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG 
CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG 
CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG 
GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC 
GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC 
CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC 
GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG 
GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC 
AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG 
ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC 
TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG 
CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT 
CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG 
AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC 
GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC 
AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC 
CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GIGYF1
Entrez Gene ID	532406
Full Name	GRB10 interacting GYF protein 1
General protein information	Preferred Names GRB10 interacting GYF protein 1 Names PERQ amino acid-rich with GYF domain-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	GIGYF1	XP_003318704.1
	GIGYF1	XP_001104969.1
Bos taurus (cattle)	GIGYF1	NP_001192927.1
Rattus norvegicus (Norway rat)	Gigyf1	NP_001100603.1
Danio rerio (zebrafish)	LOC100002679	XP_003199382.2
Xenopus tropicalis (tropical clawed frog)	gigyf1	NP_001072603.1
Homo sapiens (human)	GIGYF1	NP_072096.2
Mus musculus (house mouse)	Gigyf1	NP_113596.2
Canis lupus familiaris (dog)	GIGYF1	XP_546951.2
Danio rerio (zebrafish)	LOC568584	XP_001919939.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GIGYF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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