ANKRD50 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD50
Entrez Gene ID	527956
Full Name	ankyrin repeat domain 50
General protein information	Preferred Names ankyrin repeat domain 50 Names ankyrin repeat domain-containing protein 50
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005217603.3	XP_005217660.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_005217604.3	XP_005217661.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_010813781.2	XP_010812083.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_005217603.4	XP_005217660.1	ankyrin repeat domain-containing protein 50 isoform X1
NM_001205949.1	NP_001192878.1	ankyrin repeat domain-containing protein 50
NM_001205949.2	NP_001192878.1	ankyrin repeat domain-containing protein 50
XM_005217604.4	XP_005217661.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_010813781.3	XP_010812083.1	ankyrin repeat domain-containing protein 50 isoform X1

Pathways from BioSystems

Homologies

	ANKRD50	XP_001104758.1
Gallus gallus (chicken)	ANKRD50	XP_420618.4
Canis lupus familiaris (dog)	ANKRD50	XP_540955.2
Danio rerio (zebrafish)	si:dkey-145p14.5	XP_696361.3
Pan troglodytes (chimpanzee)	ANKRD50	XP_517429.1
Homo sapiens (human)	ANKRD50	NP_065070.1
Bos taurus (cattle)	ANKRD50	NP_001192878.1
Rattus norvegicus (Norway rat)	Ankrd50	NP_001178535.1
Xenopus tropicalis (tropical clawed frog)	ankrd50	XP_002942044.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ANKRD50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38567	NM_001205949.2 Latest version!	Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27703	XM_005217603.4 Latest version!	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27703	XM_005217604.4 Latest version!	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27703	XM_010813781.3 Latest version!	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa27703	XM_005217603.3	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa38567	NM_001205949.1	Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27703	XM_005217604.3	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa27703	XM_010813781.2	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD50 ( NM_001205949.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192878.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

Price & Availability↑

CloneID	OBa38567
Clone ID Related Accession (Same CDS sequence)	NM_001205949.1 , NM_001205949.2
Accession Version	NM_001205949.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02044718.1. On Apr 20, 2011 this sequence version replaced XM_606363.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403654, SAMN02403655 [ECO:0000348] ##Evidence-Data-END##

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG 
ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT 
GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG 
AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC 
CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA 
TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG 
AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT 
ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT 
CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC 
GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG 
AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA 
CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC 
TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA 
ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT 
GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC 
TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT 
AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA 
CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG 
GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG 
TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG 
GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA 
CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT 
GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG 
GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA 
GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG 
CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT 
TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA 
GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT 
GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA 
GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT 
GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT 
GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT 
TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA 
GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA 
TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG 
CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG 
GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT 
GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC 
TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG 
ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC 
TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC 
TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC 
ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC 
GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT 
GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT 
GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT 
CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC 
ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA 
TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA 
AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG 
CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG 
TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC 
AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG 
TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA 
ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG 
CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT 
CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT 
ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA 
GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG 
GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192878.1
CDS	1..4290
Translation	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_001205949.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205949.1

ORF Insert Sequence:

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG 
ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT 
GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG 
AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC 
CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA 
TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG 
AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT 
ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT 
CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC 
GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG 
AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA 
CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC 
TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA 
ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT 
GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC 
TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT 
AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA 
CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG 
GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG 
TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG 
GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA 
CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT 
GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG 
GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA 
GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG 
CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT 
TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA 
GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT 
GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA 
GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT 
GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT 
GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT 
TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA 
GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA 
TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG 
CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG 
GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT 
GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC 
TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG 
ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC 
TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC 
TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC 
ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC 
GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT 
GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT 
GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT 
CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC 
ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA 
TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA 
AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG 
CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG 
TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC 
AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG 
TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA 
ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG 
CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT 
CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT 
ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA 
GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG 
GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( NM_001205949.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192878.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

Price & Availability↑

CloneID	OBa38567
Clone ID Related Accession (Same CDS sequence)	NM_001205949.1 , NM_001205949.2
Accession Version	NM_001205949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-20
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Dec 21, 2018 this sequence version replaced NM_001205949.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721698.3619.1, SRR5721694.3415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG 
ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT 
GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG 
AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC 
CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA 
TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG 
AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT 
ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT 
CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC 
GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG 
AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA 
CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC 
TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA 
ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT 
GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC 
TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT 
AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA 
CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG 
GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG 
TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG 
GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA 
CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT 
GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG 
GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA 
GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG 
CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT 
TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA 
GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT 
GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA 
GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT 
GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT 
GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT 
TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA 
GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA 
TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG 
CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG 
GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT 
GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC 
TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG 
ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC 
TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC 
TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC 
ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC 
GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT 
GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT 
GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT 
CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC 
ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA 
TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA 
AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG 
CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG 
TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC 
AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG 
TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA 
ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG 
CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT 
CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT 
ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA 
GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG 
GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192878.1
CDS	1..4290
Translation	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_001205949.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205949.2

ORF Insert Sequence:

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG 
ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT 
GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG 
AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC 
CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA 
TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG 
AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT 
ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT 
CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC 
GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG 
AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA 
CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC 
TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA 
ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT 
GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC 
TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT 
AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA 
CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG 
GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG 
TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG 
GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA 
CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT 
GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG 
GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA 
GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG 
CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT 
TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA 
GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT 
GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA 
GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT 
GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT 
GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT 
TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA 
GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA 
TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG 
CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG 
GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT 
GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC 
TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG 
ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC 
TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC 
TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC 
ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC 
GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT 
GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT 
GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT 
CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC 
ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA 
TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA 
AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG 
CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG 
TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC 
AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG 
TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA 
ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG 
CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT 
CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT 
ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA 
GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG 
GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_005217603.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217660.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

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CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_005217603.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217660.1
CDS	686..5008
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_005217603.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217603.3

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_005217604.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217661.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

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CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_005217604.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217604.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217661.1
CDS	739..5061
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_005217604.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217604.3

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_010813781.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812083.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_010813781.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812083.1
CDS	883..5205
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_010813781.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813781.2

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_005217603.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217660.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_005217603.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217603.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217660.1
CDS	661..4983
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_005217603.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217603.4

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_005217604.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217661.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

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CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_005217604.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217604.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217661.1
CDS	714..5036
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_005217604.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217604.4

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_010813781.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812083.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

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CloneID	OBa27703
Clone ID Related Accession (Same CDS sequence)	XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
Accession Version	XM_010813781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812083.1
CDS	943..5265
Translation	MLANVDGKKPIMTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKTNCCNSAVNTPPLVMNSGNNASVVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPTSLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAELLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHLEMVQVLITYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSVSSKMQSLTIKSNSSGSTGGGEAQPSLRSLPNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHSLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHHSPSSEFEWSQVKPSLKSTKTNKGGKSENSNKSGSAGKKAKQNNSSQPKVLEYEMTQFDKRGPTAKSGTSAPSKQMPADSQCKIMIPSAQPEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTIQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_010813781.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813781.3

ORF Insert Sequence:

ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT 
TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC 
ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT 
GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA 
CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC 
TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT 
CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT 
CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT 
AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT 
CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC 
TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA 
TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT 
CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC 
ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA 
GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT 
CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT 
CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG 
GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA 
TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA 
GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC 
AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA 
GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA 
TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT 
GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA 
ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC 
GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG 
ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG 
GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA 
GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA 
CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG 
GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA 
CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA 
GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA 
GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT 
AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC 
GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG 
GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG 
ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG 
GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG 
TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC 
ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA 
GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA 
CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA 
GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA 
GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA 
GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT 
GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA 
CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT 
GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA 
GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC 
AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG 
CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC 
TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT 
GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA 
CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT 
CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG 
TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG 
TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT 
TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT 
TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC 
CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC 
CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG 
AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA 
GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT 
AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC 
ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA 
ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC 
CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG 
CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG 
CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT 
CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ANKRD50 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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