PBRM1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015459550.1	XP_015315036.1	protein polybromo-1 isoform X5
XM_010817828.2	XP_010816130.1	protein polybromo-1 isoform X10
XM_005222983.4	XP_005223040.1	protein polybromo-1 isoform X7
XM_024982487.1	XP_024838255.1	protein polybromo-1 isoform X7
XM_024982491.1	XP_024838259.1	protein polybromo-1 isoform X8
XM_024982494.1	XP_024838262.1	protein polybromo-1 isoform X12
XM_015459551.1	XP_015315037.1	protein polybromo-1 isoform X5
XM_010817824.2	XP_010816126.1	protein polybromo-1 isoform X7
XM_024982485.1	XP_024838253.1	protein polybromo-1 isoform X4
XM_024982486.1	XP_024838254.1	protein polybromo-1 isoform X5
XM_024982489.1	XP_024838257.1	protein polybromo-1 isoform X7
XM_024982493.1	XP_024838261.1	protein polybromo-1 isoform X10
XM_010817827.3	XP_010816129.1	protein polybromo-1 isoform X13
XM_010817830.1	XP_010816132.1	protein polybromo-1 isoform X18
XM_010817823.1	XP_010816125.1	protein polybromo-1 isoform X6
XM_010817827.2	XP_010816129.1	protein polybromo-1 isoform X9
XM_005222983.3	XP_005223040.1	protein polybromo-1 isoform X5
XM_010817817.2	XP_010816119.1	protein polybromo-1 isoform X2
XM_010817818.2	XP_010816120.1	protein polybromo-1 isoform X3
XM_015459548.1	XP_015315034.1	protein polybromo-1 isoform X1
XM_024982488.1	XP_024838256.1	protein polybromo-1 isoform X7
XM_024982495.1	XP_024838263.1	protein polybromo-1 isoform X16
NM_001305012.1	NP_001291941.1	protein polybromo-1
XM_010817819.3	XP_010816121.1	protein polybromo-1 isoform X6
XM_010817826.1	XP_010816128.1	protein polybromo-1 isoform X11
XM_005222979.3	XP_005223036.1	protein polybromo-1 isoform X1
XM_010817819.2	XP_010816121.1	protein polybromo-1 isoform X4
XM_005222979.4	XP_005223036.1	protein polybromo-1 isoform X1
XM_024982483.1	XP_024838251.1	protein polybromo-1 isoform X2
XM_024982484.1	XP_024838252.1	protein polybromo-1 isoform X3
XM_024982490.1	XP_024838258.1	protein polybromo-1 isoform X7
XM_024982492.1	XP_024838260.1	protein polybromo-1 isoform X9
XM_010817828.3	XP_010816130.1	protein polybromo-1 isoform X14
XM_010817829.1	XP_010816131.1	protein polybromo-1 isoform X15
XM_024982497.1	XP_024838265.1	protein polybromo-1 isoform X17

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228783	XM_024982483.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228784	XM_024982484.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228785	XM_024982492.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228786	XM_024982493.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228787	XM_024982494.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa228788	XM_024982495.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa228789	XM_024982497.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa31683	NM_001305012.1 Latest version!	Bos taurus polybromo 1 (PBRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31679	XM_015459548.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31679	XM_005222979.4 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31681	XM_010817817.2 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31681	XM_024982485.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31682	XM_010817819.3 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_024982489.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_024982488.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_024982487.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_024982490.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_015459550.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_005222983.4 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31683	XM_015459551.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31684	XM_024982491.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31684	XM_010817823.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31685	XM_010817824.2 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31686	XM_010817826.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31687	XM_010817827.3 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa31688	XM_010817828.3 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa31689	XM_010817829.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa31690	XM_010817830.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa46598	XM_024982486.1 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa46598	XM_010817818.2 Latest version!	Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa31687	XM_010817827.2	Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa31683	XM_005222983.3	Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31682	XM_010817819.2	Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31679	XM_005222979.3	Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31688	XM_010817828.2	Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

PBRM1 ( XM_024982483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838251.1 Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228783
Clone ID Related Accession (Same CDS sequence)	XM_024982483.1
Accession Version	XM_024982483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838251.1
CDS	57..5228
Translation	MRRLAFRGAGCALVKLKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982483.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GTTGGATTCC 
ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982484.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838252.1 Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.

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CloneID	OBa228784
Clone ID Related Accession (Same CDS sequence)	XM_024982484.1
Accession Version	XM_024982484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838252.1
CDS	79..5250
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKRAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982484.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982492.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838260.1 Bos taurus polybromo 1 (PBRM1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa228785
Clone ID Related Accession (Same CDS sequence)	XM_024982492.1
Accession Version	XM_024982492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGTA AAAAGAACAT AAGAAAGCAG 
CGAATGAAAA TCTTATTCAA TGTTGTTCTC GAAGCTCGTG AGCCAGGCTC AGGCAGAAGA 
CTTTGTGATC TATTTATGGT TAAACCATCC AAAAAGGACT ATCCTGATTA TTATAAAATC 
ATCTTGGAGC CAATGGACTT GAAAATAATT GAGCATAACA TCCGCAATGA CAAATATGCG 
GGGGAGGAGG GAATGATAGA GGACATGAAG CTGATGTTCC GGAACGCCAG GCACTACAAC 
GAGGAGGGCT CCCAGGTGTA CAACGATGCA CACATCCTGG AGAAGTTACT CAAGGATAAA 
AGGAAAGAGC TGGGCCCACT GCCTGATGAT GATGACATGG CATCTCCCAA ACTCAAGCTG 
AGTAGGAAGA GTGGTATTTC TCCTAAAAAA TCAAAATACA TGACTCCAAT GCAGCAGAAA 
CTAAATGAAG TCTATGAAGC TGTAAAGAAC TATACTGATA AGAGGGGTCG CCGCCTCAGT 
GCCATATTTC TGAGGCTTCC CTCCAGATCC GAGCTGCCTG ACTACTATTT GACCATTAAA 
AAGCCCATGG ACATGGAAAA AATTCGAAGT CACATGATGG CCAACAAATA CCAAGATATA 
GACTCCATGG TTGAGGACTT CGTCATGATG TTTAACAATG CCTGTACATA CAATGAGCCC 
GAGTCTTTGA TCTACAAAGA TGCCCTTGTC CTACACAAAG TGCTGCTTGA AACTCGGAGA 
GACTTAGAAG GAGATGAGGA CTCGCATGTC CCAAATGTAA CCTTGCTGAT TCAGGAGCTT 
ATCCATAATC TTTTTGTGTC AGTCATGAGT CATCAGGATG ATGAAGGAAG ATGCTACAGT 
GATTCCTTAG CAGAAATTCC TGCTGTGGAT CCCAGCTTTC CAAACAAACC TCCTCTTACT 
TTTGACATAA TTAGGAAGAA TGTTGAAAAT AACCGCTACC GACGGCTTGA TTTATTTCAA 
GAGCATATGT TTGAAGTATT GGAGAGGGCA AGAAGGATGA ATCGGACAGA TTCTGAAATA 
TATGAGGATG CGGTAGAACT TCAGCAGTTT TTTATTAAAA TTCGTGATGA ACTCTGCAAA 
AATGGAGAGA TCCTTCTTTC ACCAGCACTC AGCTATACCA CAAAACATTT GCATAATGAT 
GTGGAGAAAG AAAAGAAGGA AAAACTGCCA AAAGAAATAG AGGAAGATAA ACTAAAACGA 
GAAGAAGAAA AAAGAGAAGC TGAAAAGAGT GAAGATTCCT CAGGAGCTGC AGGCCTCTCA 
GGCTTACATC GCACATACAG CCAGGACTGC AGCTTTAAGA ACAGCATGTA CCATGTTGGA 
GATTACGTCT ATGTGGAGCC CTCAGAGGTC AATCTACAAC CGCATATCGT CTGTATTGAG 
AGACTGTGGG AAGATTCTGC TGGTGAAAAA TGGTTGTATG GCTGTTGGTT TTATCGGCCA 
AATGAAACAT TCCATTTGGC TACACGGAAA TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC 
TATTACAATA AAGTTCCTGT TAGTAAAATT CTAGGAAAAT GTGTGGTCAT GTTTGTCAAG 
GAATACTTTA AATTATGCCC AGAAAATTTT CGAGATGAGG ATGTTTTTGT CTGTGAATCA 
CGATATTCTG CCAAAACCAA ATCTTTTAAG AAAATTAAAC TGTGGACCAT GCCCGTCAGC 
TCGGTTAGGT TTGTCCCTCG AGATGTGCCC TTGCCTGTGG TCCGAGTGGC CTCTGTATTT 
GCAAACGCAG ATAAAGCGGA TGATGAGAAG AATACAGACA ACTCAGAGGA CAGTCGAACT 
GAAGACAGTT GTAACTTGGA AAAGGAGAAA GAAGATGTCC CTGTGGAAAT GTCGAATGGT 
GAACCAGGTT GCCACTACTT TGAGCAGCTC CGGTATAATG ACATGTGGCT GAAGGTTGGC 
GACTGTGTCT TCATCAAGTC CCATGGGCTA GTGCGTCCTC GTGTTGGCAG AATTGAAAAG 
GTATGGGTCC GAGATGGAGC TGCGTATTTT TATGGCCCCA TCTTCATTCA CCCAGAAGAG 
ACGGAACATG AGCCGACAAA AATGTTCTAC AAGAAGGAAG TGTTTTTGAG TAATCTGGAA 
GAAACCTGTC CCATGACATG TATTCTAGGA AAATGTGCTG TGTTGTCATT CAAGGACTTC 
CTCTCCTGCA GGCCAACTGA AATTCCAGAA AATGACATTC TGCTTTGTGA GAGCCGCTAC 
AACGAGAGTG ACAAGCAGAT GAAGAAATTT AAGGGACTGA AGAGATTTTC ACTGTCTGCT 
AAAGTGGTAG ATGATGAAAT TTATTACTTC AGAAAACCAA TTGTTCCTCA GAAGGAGCCC 
TCACCTTTAT TGGAAAAGAA GATCCAGTTG CTAGAAGCTA AATTTGCTGA GTTAGAAGGT 
GGAGATGATG ATATTGAAGA GATGGGAGAA GAGGATAGTG AGGTCATTGA GCCTCCCTCT 
CTACCTCAAC TTCAGACTCC CCTGGCCAGT GAGCTGGATC TCATGCCCTA CACACCCCCA 
CAGTCTACGC CCAAATCTGC CAAAGGCAGT GCAAAGAAGG AAGGCTCCAA ACGGAAGATC 
AACATGAGTG GCTACATCCT GTTCAGCAGT GAGATGAGAG CTGTGATTAA GGCCCAGCAC 
CCAGACTATT CTTTTGGGGA GCTCAGCCGA CTGGTGGGGA CAGAGTGGAG AAACCTTGAG 
ACAGCCAAGA AAGCAGAATA TGAAGAGCGG GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA 
GAGCGAGCAG CACAGCAACA GCAGCCGAGT GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG 
GCTCTCATGG GGGTGGTGCC ACCACCAACA CCAATGGGGA TGCTCAATCA GCAGTTGACA 
CCTGTTGCAG GCATGATGGG TGGCTATCCG CCAGGCCTTC CACCTTTGCA GGGCCCAGTT 
GATGGCCTTG TTAGCATGGG CAGCATGCAG CCACTTCACC CTGGGGGGCC TCCACCCCAC 
CATCTTCCGC CAGGTGTGCC CGGCCTCCCG GGCATCCCAC CACCGGGTGT GATGAACCAA 
GGAGTGGCCC CTATGGTAGG GACTCCAGCA CCAGGTGGAA GTCCGTATGG ACAGCAGGTA 
GGTGTTCTGG GGCCTCCAGG GCAGCAGGCA CCACCTCCAT ATCCTGGCCC ACATCCTGCT 
GGCCCCCCTG TCATACAGCA GCCAACAACA CCCATGTTTG TGGCTCCCCC ACCGAAGACC 
CAACGGCTTC TCCACTCAGA GGCCTACCTA AAATACATTG AAGGACTCAG TGCTGAGTCC 
AACAGCATTA GCAAGTGGGA CCAGACTCTG GCAGCTCGAA GACGTGATGT CCATCTGTCG 
AAAGAACAGG AGAGCCGCCT TCCCTCTCAC TGGCTAAAAA GTAAAGGGGC CCACACCACC 
ATGGCAGATG CCCTATGGCG CCTTCGGGAT CTGATGCTCC GAGACACCCT CAACATTCGC 
CAAGCATACA ATCTAGAAAA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838260.1
CDS	199..5265
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982492.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982492.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGTA AAAAGAACAT AAGAAAGCAG 
CGAATGAAAA TCTTATTCAA TGTTGTTCTC GAAGCTCGTG AGCCAGGCTC AGGCAGAAGA 
CTTTGTGATC TATTTATGGT TAAACCATCC AAAAAGGACT ATCCTGATTA TTATAAAATC 
ATCTTGGAGC CAATGGACTT GAAAATAATT GAGCATAACA TCCGCAATGA CAAATATGCG 
GGGGAGGAGG GAATGATAGA GGACATGAAG CTGATGTTCC GGAACGCCAG GCACTACAAC 
GAGGAGGGCT CCCAGGTGTA CAACGATGCA CACATCCTGG AGAAGTTACT CAAGGATAAA 
AGGAAAGAGC TGGGCCCACT GCCTGATGAT GATGACATGG CATCTCCCAA ACTCAAGCTG 
AGTAGGAAGA GTGGTATTTC TCCTAAAAAA TCAAAATACA TGACTCCAAT GCAGCAGAAA 
CTAAATGAAG TCTATGAAGC TGTAAAGAAC TATACTGATA AGAGGGGTCG CCGCCTCAGT 
GCCATATTTC TGAGGCTTCC CTCCAGATCC GAGCTGCCTG ACTACTATTT GACCATTAAA 
AAGCCCATGG ACATGGAAAA AATTCGAAGT CACATGATGG CCAACAAATA CCAAGATATA 
GACTCCATGG TTGAGGACTT CGTCATGATG TTTAACAATG CCTGTACATA CAATGAGCCC 
GAGTCTTTGA TCTACAAAGA TGCCCTTGTC CTACACAAAG TGCTGCTTGA AACTCGGAGA 
GACTTAGAAG GAGATGAGGA CTCGCATGTC CCAAATGTAA CCTTGCTGAT TCAGGAGCTT 
ATCCATAATC TTTTTGTGTC AGTCATGAGT CATCAGGATG ATGAAGGAAG ATGCTACAGT 
GATTCCTTAG CAGAAATTCC TGCTGTGGAT CCCAGCTTTC CAAACAAACC TCCTCTTACT 
TTTGACATAA TTAGGAAGAA TGTTGAAAAT AACCGCTACC GACGGCTTGA TTTATTTCAA 
GAGCATATGT TTGAAGTATT GGAGAGGGCA AGAAGGATGA ATCGGACAGA TTCTGAAATA 
TATGAGGATG CGGTAGAACT TCAGCAGTTT TTTATTAAAA TTCGTGATGA ACTCTGCAAA 
AATGGAGAGA TCCTTCTTTC ACCAGCACTC AGCTATACCA CAAAACATTT GCATAATGAT 
GTGGAGAAAG AAAAGAAGGA AAAACTGCCA AAAGAAATAG AGGAAGATAA ACTAAAACGA 
GAAGAAGAAA AAAGAGAAGC TGAAAAGAGT GAAGATTCCT CAGGAGCTGC AGGCCTCTCA 
GGCTTACATC GCACATACAG CCAGGACTGC AGCTTTAAGA ACAGCATGTA CCATGTTGGA 
GATTACGTCT ATGTGGAGCC CTCAGAGGTC AATCTACAAC CGCATATCGT CTGTATTGAG 
AGACTGTGGG AAGATTCTGC TGGTGAAAAA TGGTTGTATG GCTGTTGGTT TTATCGGCCA 
AATGAAACAT TCCATTTGGC TACACGGAAA TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC 
TATTACAATA AAGTTCCTGT TAGTAAAATT CTAGGAAAAT GTGTGGTCAT GTTTGTCAAG 
GAATACTTTA AATTATGCCC AGAAAATTTT CGAGATGAGG ATGTTTTTGT CTGTGAATCA 
CGATATTCTG CCAAAACCAA ATCTTTTAAG AAAATTAAAC TGTGGACCAT GCCCGTCAGC 
TCGGTTAGGT TTGTCCCTCG AGATGTGCCC TTGCCTGTGG TCCGAGTGGC CTCTGTATTT 
GCAAACGCAG ATAAAGCGGA TGATGAGAAG AATACAGACA ACTCAGAGGA CAGTCGAACT 
GAAGACAGTT GTAACTTGGA AAAGGAGAAA GAAGATGTCC CTGTGGAAAT GTCGAATGGT 
GAACCAGGTT GCCACTACTT TGAGCAGCTC CGGTATAATG ACATGTGGCT GAAGGTTGGC 
GACTGTGTCT TCATCAAGTC CCATGGGCTA GTGCGTCCTC GTGTTGGCAG AATTGAAAAG 
GTATGGGTCC GAGATGGAGC TGCGTATTTT TATGGCCCCA TCTTCATTCA CCCAGAAGAG 
ACGGAACATG AGCCGACAAA AATGTTCTAC AAGAAGGAAG TGTTTTTGAG TAATCTGGAA 
GAAACCTGTC CCATGACATG TATTCTAGGA AAATGTGCTG TGTTGTCATT CAAGGACTTC 
CTCTCCTGCA GGCCAACTGA AATTCCAGAA AATGACATTC TGCTTTGTGA GAGCCGCTAC 
AACGAGAGTG ACAAGCAGAT GAAGAAATTT AAGGGACTGA AGAGATTTTC ACTGTCTGCT 
AAAGTGGTAG ATGATGAAAT TTATTACTTC AGAAAACCAA TTGTTCCTCA GAAGGAGCCC 
TCACCTTTAT TGGAAAAGAA GATCCAGTTG CTAGAAGCTA AATTTGCTGA GTTAGAAGGT 
GGAGATGATG ATATTGAAGA GATGGGAGAA GAGGATAGTG AGGTCATTGA GCCTCCCTCT 
CTACCTCAAC TTCAGACTCC CCTGGCCAGT GAGCTGGATC TCATGCCCTA CACACCCCCA 
CAGTCTACGC CCAAATCTGC CAAAGGCAGT GCAAAGAAGG AAGGCTCCAA ACGGAAGATC 
AACATGAGTG GCTACATCCT GTTCAGCAGT GAGATGAGAG CTGTGATTAA GGCCCAGCAC 
CCAGACTATT CTTTTGGGGA GCTCAGCCGA CTGGTGGGGA CAGAGTGGAG AAACCTTGAG 
ACAGCCAAGA AAGCAGAATA TGAAGAGCGG GCAGCTAAAG TTGCTGAGCA GCAGGAGAGA 
GAGCGAGCAG CACAGCAACA GCAGCCGAGT GCTTCTCCCC GAGCAGGCAC CCCTGTGGGG 
GCTCTCATGG GGGTGGTGCC ACCACCAACA CCAATGGGGA TGCTCAATCA GCAGTTGACA 
CCTGTTGCAG GCATGATGGG TGGCTATCCG CCAGGCCTTC CACCTTTGCA GGGCCCAGTT 
GATGGCCTTG TTAGCATGGG CAGCATGCAG CCACTTCACC CTGGGGGGCC TCCACCCCAC 
CATCTTCCGC CAGGTGTGCC CGGCCTCCCG GGCATCCCAC CACCGGGTGT GATGAACCAA 
GGAGTGGCCC CTATGGTAGG GACTCCAGCA CCAGGTGGAA GTCCGTATGG ACAGCAGGTA 
GGTGTTCTGG GGCCTCCAGG GCAGCAGGCA CCACCTCCAT ATCCTGGCCC ACATCCTGCT 
GGCCCCCCTG TCATACAGCA GCCAACAACA CCCATGTTTG TGGCTCCCCC ACCGAAGACC 
CAACGGCTTC TCCACTCAGA GGCCTACCTA AAATACATTG AAGGACTCAG TGCTGAGTCC 
AACAGCATTA GCAAGTGGGA CCAGACTCTG GCAGCTCGAA GACGTGATGT CCATCTGTCG 
AAAGAACAGG AGAGCCGCCT TCCCTCTCAC TGGCTAAAAA GTAAAGGGGC CCACACCACC 
ATGGCAGATG CCCTATGGCG CCTTCGGGAT CTGATGCTCC GAGACACCCT CAACATTCGC 
CAAGCATACA ATCTAGAAAA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982493.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838261.1 Bos taurus polybromo 1 (PBRM1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa228786
Clone ID Related Accession (Same CDS sequence)	XM_024982493.1
Accession Version	XM_024982493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG TAATAAAAGA 
GCAGTCCAAG GGGGTCGTTT ATCTGCAATT ACCATGGCAC TTCAGTATGG CTCAGAAAGT 
GAAGAGGATG CTGCTTTAGC TGCTGCGCGA TATGAAGAAG GAGAATCTGA AGCAGAGAGC 
ATTACTTCAT TTATGGATGT TTCAAATCCT TTTTATCAGC TTTATGACAC AGTTCGGAGC 
TGTCGGAATA ACCAAGGCCA GCTAATAGCT GAACCTTTTT TCCATTTGCC TTCAAAGAAA 
AAATATCCTG ATTATTATCA GCAAATTAAA ACACCCATAT CACTACAACA GATCAGAACA 
AAGCTAAAAA ACCAAGAATA TGAAACTTTA GATCATTTGG AGTGTGATCT GAATTTAATG 
TTTGAAAATG CCAAACGCTA TAATGTTCCC AACTCGGCCA TCTACAAGCG AGTTCTGAAA 
CTGCAGCAAG TCATGCAGGC AAAGAAGAAA GAGCTTGCTA GGAGAGACGA CATTGAGGAT 
GGAGACAGCA TGATTTCTTC AGCCACCTCT GATACTGGTA GTGCCAAAAG AAAAAGTAAA 
AAGAACATAA GAAAGCAGCG AATGAAAATC TTATTCAATG TTGTTCTCGA AGCTCGTGAG 
CCAGGCTCAG GCAGAAGACT TTGTGATCTA TTTATGGTTA AACCATCCAA AAAGGACTAT 
CCTGATTATT ATAAAATCAT CTTGGAGCCA ATGGACTTGA AAATAATTGA GCATAACATC 
CGCAATGACA AATATGCGGG GGAGGAGGGA ATGATAGAGG ACATGAAGCT GATGTTCCGG 
AACGCCAGGC ACTACAACGA GGAGGGCTCC CAGGTGTACA ACGATGCACA CATCCTGGAG 
AAGTTACTCA AGGATAAAAG GAAAGAGCTG GGCCCACTGC CTGATGATGA TGACATGGCA 
TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT GGTATTTCTC CTAAAAAATC AAAATACATG 
ACTCCAATGC AGCAGAAACT AAATGAAGTC TATGAAGCTG TAAAGAACTA TACTGATAAG 
AGGGGTCGCC GCCTCAGTGC CATATTTCTG AGGCTTCCCT CCAGATCCGA GCTGCCTGAC 
TACTATTTGA CCATTAAAAA GCCCATGGAC ATGGAAAAAA TTCGAAGTCA CATGATGGCC 
AACAAATACC AAGATATAGA CTCCATGGTT GAGGACTTCG TCATGATGTT TAACAATGCC 
TGTACATACA ATGAGCCCGA GTCTTTGATC TACAAAGATG CCCTTGTCCT ACACAAAGTG 
CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA GATGAGGACT CGCATGTCCC AAATGTAACC 
TTGCTGATTC AGGAGCTTAT CCATAATCTT TTTGTGTCAG TCATGAGTCA TCAGGATGAT 
GAAGGAAGAT GCTACAGTGA TTCCTTAGCA GAAATTCCTG CTGTGGATCC CAGCTTTCCA 
AACAAACCTC CTCTTACTTT TGACATAATT AGGAAGAATG TTGAAAATAA CCGCTACCGA 
CGGCTTGATT TATTTCAAGA GCATATGTTT GAAGTATTGG AGAGGGCAAG AAGGATGAAT 
CGGACAGATT CTGAAATATA TGAGGATGCG GTAGAACTTC AGCAGTTTTT TATTAAAATT 
CGTGATGAAC TCTGCAAAAA TGGAGAGATC CTTCTTTCAC CAGCACTCAG CTATACCACA 
AAACATTTGC ATAATGATGT GGAGAAAGAA AAGAAGGAAA AACTGCCAAA AGAAATAGAG 
GAAGATAAAC TAAAACGAGA AGAAGAAAAA AGAGAAGCTG AAAAGAGTGA AGATTCCTCA 
GGAGCTGCAG GCCTCTCAGG CTTACATCGC ACATACAGCC AGGACTGCAG CTTTAAGAAC 
AGCATGTACC ATGTTGGAGA TTACGTCTAT GTGGAGCCCT CAGAGGTCAA TCTACAACCG 
CATATCGTCT GTATTGAGAG ACTGTGGGAA GATTCTGCTG GTGAAAAATG GTTGTATGGC 
TGTTGGTTTT ATCGGCCAAA TGAAACATTC CATTTGGCTA CACGGAAATT TCTAGAAAAA 
GAAGTTTTTA AGAGTGACTA TTACAATAAA GTTCCTGTTA GTAAAATTCT AGGAAAATGT 
GTGGTCATGT TTGTCAAGGA ATACTTTAAA TTATGCCCAG AAAATTTTCG AGATGAGGAT 
GTTTTTGTCT GTGAATCACG ATATTCTGCC AAAACCAAAT CTTTTAAGAA AATTAAACTG 
TGGACCATGC CCGTCAGCTC GGTTAGGTTT GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC 
CGAGTGGCCT CTGTATTTGC AAACGCAGAT AAAGCGGATG ATGAGAAGAA TACAGACAAC 
TCAGAGGACA GTCGAACTGA AGACAGTTGT AACTTGGAAA AGGAGAAAGA AGATGTCCCT 
GTGGAAATGT CGAATGGTGA ACCAGGTTGC CACTACTTTG AGCAGCTCCG GTATAATGAC 
ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT 
GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA GATGGAGCTG CGTATTTTTA TGGCCCCATC 
TTCATTCACC CAGAAGAGAC GGAACATGAG CCGACAAAAA TGTTCTACAA GAAGGAAGTG 
TTTTTGAGTA ATCTGGAAGA AACCTGTCCC ATGACATGTA TTCTAGGAAA ATGTGCTGTG 
TTGTCATTCA AGGACTTCCT CTCCTGCAGG CCAACTGAAA TTCCAGAAAA TGACATTCTG 
CTTTGTGAGA GCCGCTACAA CGAGAGTGAC AAGCAGATGA AGAAATTTAA GGGACTGAAG 
AGATTTTCAC TGTCTGCTAA AGTGGTAGAT GATGAAATTT ATTACTTCAG AAAACCAATT 
GTTCCTCAGA AGGAGCCCTC ACCTTTATTG GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA 
TTTGCTGAGT TAGAAGGTGG AGATGATGAT ATTGAAGAGA TGGGAGAAGA GGATAGTGAG 
GTCATTGAGC CTCCCTCTCT ACCTCAACTT CAGACTCCCC TGGCCAGTGA GCTGGATCTC 
ATGCCCTACA CACCCCCACA GTCTACGCCC AAATCTGCCA AAGGCAGTGC AAAGAAGGAA 
GGCTCCAAAC GGAAGATCAA CATGAGTGGC TACATCCTGT TCAGCAGTGA GATGAGAGCT 
GTGATTAAGG CCCAGCACCC AGACTATTCT TTTGGGGAGC TCAGCCGACT GGTGGGGACA 
GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838261.1
CDS	79..5103
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982493.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982493.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG TAATAAAAGA 
GCAGTCCAAG GGGGTCGTTT ATCTGCAATT ACCATGGCAC TTCAGTATGG CTCAGAAAGT 
GAAGAGGATG CTGCTTTAGC TGCTGCGCGA TATGAAGAAG GAGAATCTGA AGCAGAGAGC 
ATTACTTCAT TTATGGATGT TTCAAATCCT TTTTATCAGC TTTATGACAC AGTTCGGAGC 
TGTCGGAATA ACCAAGGCCA GCTAATAGCT GAACCTTTTT TCCATTTGCC TTCAAAGAAA 
AAATATCCTG ATTATTATCA GCAAATTAAA ACACCCATAT CACTACAACA GATCAGAACA 
AAGCTAAAAA ACCAAGAATA TGAAACTTTA GATCATTTGG AGTGTGATCT GAATTTAATG 
TTTGAAAATG CCAAACGCTA TAATGTTCCC AACTCGGCCA TCTACAAGCG AGTTCTGAAA 
CTGCAGCAAG TCATGCAGGC AAAGAAGAAA GAGCTTGCTA GGAGAGACGA CATTGAGGAT 
GGAGACAGCA TGATTTCTTC AGCCACCTCT GATACTGGTA GTGCCAAAAG AAAAAGTAAA 
AAGAACATAA GAAAGCAGCG AATGAAAATC TTATTCAATG TTGTTCTCGA AGCTCGTGAG 
CCAGGCTCAG GCAGAAGACT TTGTGATCTA TTTATGGTTA AACCATCCAA AAAGGACTAT 
CCTGATTATT ATAAAATCAT CTTGGAGCCA ATGGACTTGA AAATAATTGA GCATAACATC 
CGCAATGACA AATATGCGGG GGAGGAGGGA ATGATAGAGG ACATGAAGCT GATGTTCCGG 
AACGCCAGGC ACTACAACGA GGAGGGCTCC CAGGTGTACA ACGATGCACA CATCCTGGAG 
AAGTTACTCA AGGATAAAAG GAAAGAGCTG GGCCCACTGC CTGATGATGA TGACATGGCA 
TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT GGTATTTCTC CTAAAAAATC AAAATACATG 
ACTCCAATGC AGCAGAAACT AAATGAAGTC TATGAAGCTG TAAAGAACTA TACTGATAAG 
AGGGGTCGCC GCCTCAGTGC CATATTTCTG AGGCTTCCCT CCAGATCCGA GCTGCCTGAC 
TACTATTTGA CCATTAAAAA GCCCATGGAC ATGGAAAAAA TTCGAAGTCA CATGATGGCC 
AACAAATACC AAGATATAGA CTCCATGGTT GAGGACTTCG TCATGATGTT TAACAATGCC 
TGTACATACA ATGAGCCCGA GTCTTTGATC TACAAAGATG CCCTTGTCCT ACACAAAGTG 
CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA GATGAGGACT CGCATGTCCC AAATGTAACC 
TTGCTGATTC AGGAGCTTAT CCATAATCTT TTTGTGTCAG TCATGAGTCA TCAGGATGAT 
GAAGGAAGAT GCTACAGTGA TTCCTTAGCA GAAATTCCTG CTGTGGATCC CAGCTTTCCA 
AACAAACCTC CTCTTACTTT TGACATAATT AGGAAGAATG TTGAAAATAA CCGCTACCGA 
CGGCTTGATT TATTTCAAGA GCATATGTTT GAAGTATTGG AGAGGGCAAG AAGGATGAAT 
CGGACAGATT CTGAAATATA TGAGGATGCG GTAGAACTTC AGCAGTTTTT TATTAAAATT 
CGTGATGAAC TCTGCAAAAA TGGAGAGATC CTTCTTTCAC CAGCACTCAG CTATACCACA 
AAACATTTGC ATAATGATGT GGAGAAAGAA AAGAAGGAAA AACTGCCAAA AGAAATAGAG 
GAAGATAAAC TAAAACGAGA AGAAGAAAAA AGAGAAGCTG AAAAGAGTGA AGATTCCTCA 
GGAGCTGCAG GCCTCTCAGG CTTACATCGC ACATACAGCC AGGACTGCAG CTTTAAGAAC 
AGCATGTACC ATGTTGGAGA TTACGTCTAT GTGGAGCCCT CAGAGGTCAA TCTACAACCG 
CATATCGTCT GTATTGAGAG ACTGTGGGAA GATTCTGCTG GTGAAAAATG GTTGTATGGC 
TGTTGGTTTT ATCGGCCAAA TGAAACATTC CATTTGGCTA CACGGAAATT TCTAGAAAAA 
GAAGTTTTTA AGAGTGACTA TTACAATAAA GTTCCTGTTA GTAAAATTCT AGGAAAATGT 
GTGGTCATGT TTGTCAAGGA ATACTTTAAA TTATGCCCAG AAAATTTTCG AGATGAGGAT 
GTTTTTGTCT GTGAATCACG ATATTCTGCC AAAACCAAAT CTTTTAAGAA AATTAAACTG 
TGGACCATGC CCGTCAGCTC GGTTAGGTTT GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC 
CGAGTGGCCT CTGTATTTGC AAACGCAGAT AAAGCGGATG ATGAGAAGAA TACAGACAAC 
TCAGAGGACA GTCGAACTGA AGACAGTTGT AACTTGGAAA AGGAGAAAGA AGATGTCCCT 
GTGGAAATGT CGAATGGTGA ACCAGGTTGC CACTACTTTG AGCAGCTCCG GTATAATGAC 
ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT 
GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA GATGGAGCTG CGTATTTTTA TGGCCCCATC 
TTCATTCACC CAGAAGAGAC GGAACATGAG CCGACAAAAA TGTTCTACAA GAAGGAAGTG 
TTTTTGAGTA ATCTGGAAGA AACCTGTCCC ATGACATGTA TTCTAGGAAA ATGTGCTGTG 
TTGTCATTCA AGGACTTCCT CTCCTGCAGG CCAACTGAAA TTCCAGAAAA TGACATTCTG 
CTTTGTGAGA GCCGCTACAA CGAGAGTGAC AAGCAGATGA AGAAATTTAA GGGACTGAAG 
AGATTTTCAC TGTCTGCTAA AGTGGTAGAT GATGAAATTT ATTACTTCAG AAAACCAATT 
GTTCCTCAGA AGGAGCCCTC ACCTTTATTG GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA 
TTTGCTGAGT TAGAAGGTGG AGATGATGAT ATTGAAGAGA TGGGAGAAGA GGATAGTGAG 
GTCATTGAGC CTCCCTCTCT ACCTCAACTT CAGACTCCCC TGGCCAGTGA GCTGGATCTC 
ATGCCCTACA CACCCCCACA GTCTACGCCC AAATCTGCCA AAGGCAGTGC AAAGAAGGAA 
GGCTCCAAAC GGAAGATCAA CATGAGTGGC TACATCCTGT TCAGCAGTGA GATGAGAGCT 
GTGATTAAGG CCCAGCACCC AGACTATTCT TTTGGGGAGC TCAGCCGACT GGTGGGGACA 
GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA GCAGAATATG AAGAGCGGGC AGCTAAAGTT 
GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA CAGCAACAGC AGCCGAGTGC TTCTCCCCGA 
GCAGGCACCC CTGTGGGGGC TCTCATGGGG GTGGTGCCAC CACCAACACC AATGGGGATG 
CTCAATCAGC AGTTGACACC TGTTGCAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982494.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838262.1 Bos taurus polybromo 1 (PBRM1), transcript variant X17, mRNA.

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CloneID	OBa228787
Clone ID Related Accession (Same CDS sequence)	XM_024982494.1
Accession Version	XM_024982494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGTAAT 
AAAAGAGCAG TCCAAGGGGG TCGTTTATCT GCAATTACCA TGGCACTTCA GTATGGCTCA 
GAAAGTGAAG AGGATGCTGC TTTAGCTGCT GCGCGATATG AAGAAGGAGA ATCTGAAGCA 
GAGAGCATTA CTTCATTTAT GGATGTTTCA AATCCTTTTT ATCAGCTTTA TGACACAGTT 
CGGAGCTGTC GGAATAACCA AGGCCAGCTA ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA 
AAGAAAAAAT ATCCTGATTA TTATCAGCAA ATTAAAACAC CCATATCACT ACAACAGATC 
AGAACAAAGC TAAAAAACCA AGAATATGAA ACTTTAGATC ATTTGGAGTG TGATCTGAAT 
TTAATGTTTG AAAATGCCAA ACGCTATAAT GTTCCCAACT CGGCCATCTA CAAGCGAGTT 
CTGAAACTGC AGCAAGTCAT GCAGGCAAAG AAGAAAGAGC TTGCTAGGAG AGACGACATT 
GAGGATGGAG ACAGCATGAT TTCTTCAGCC ACCTCTGATA CTGGTAGTGC CAAAAGAAAA 
AGTAAAAAGA ACATAAGAAA GCAGCGAATG AAAATCTTAT TCAATGTTGT TCTCGAAGCT 
CGTGAGCCAG GCTCAGGCAG AAGACTTTGT GATCTATTTA TGGTTAAACC ATCCAAAAAG 
GACTATCCTG ATTATTATAA AATCATCTTG GAGCCAATGG ACTTGAAAAT AATTGAGCAT 
AACATCCGCA ATGACAAATA TGCGGGGGAG GAGGGAATGA TAGAGGACAT GAAGCTGATG 
TTCCGGAACG CCAGGCACTA CAACGAGGAG GGCTCCCAGG TGTACAACGA TGCACACATC 
CTGGAGAAGT TACTCAAGGA TAAAAGGAAA GAGCTGGGCC CACTGCCTGA TGATGATGAC 
ATGGCATCTC CCAAACTCAA GCTGAGTAGG AAGAGTGGTA TTTCTCCTAA AAAATCAAAA 
TACATGACTC CAATGCAGCA GAAACTAAAT GAAGTCTATG AAGCTGTAAA GAACTATACT 
GATAAGAGGG GTCGCCGCCT CAGTGCCATA TTTCTGAGGC TTCCCTCCAG ATCCGAGCTG 
CCTGACTACT ATTTGACCAT TAAAAAGCCC ATGGACATGG AAAAAATTCG AAGTCACATG 
ATGGCCAACA AATACCAAGA TATAGACTCC ATGGTTGAGG ACTTCGTCAT GATGTTTAAC 
AATGCCTGTA CATACAATGA GCCCGAGTCT TTGATCTACA AAGATGCCCT TGTCCTACAC 
AAAGTGCTGC TTGAAACTCG GAGAGACTTA GAAGGAGATG AGGACTCGCA TGTCCCAAAT 
GTAACCTTGC TGATTCAGGA GCTTATCCAT AATCTTTTTG TGTCAGTCAT GAGTCATCAG 
GATGATGAAG GAAGATGCTA CAGTGATTCC TTAGCAGAAA TTCCTGCTGT GGATCCCAGC 
TTTCCAAACA AACCTCCTCT TACTTTTGAC ATAATTAGGA AGAATGTTGA AAATAACCGC 
TACCGACGGC TTGATTTATT TCAAGAGCAT ATGTTTGAAG TATTGGAGAG GGCAAGAAGG 
ATGAATCGGA CAGATTCTGA AATATATGAG GATGCGGTAG AACTTCAGCA GTTTTTTATT 
AAAATTCGTG ATGAACTCTG CAAAAATGGA GAGATCCTTC TTTCACCAGC ACTCAGCTAT 
ACCACAAAAC ATTTGCATAA TGATGTGGAG AAAGAAAAGA AGGAAAAACT GCCAAAAGAA 
ATAGAGGAAG ATAAACTAAA ACGAGAAGAA GAAAAAAGAG AAGCTGAAAA GAGTGAAGAT 
TCCTCAGGAG CTGCAGGCCT CTCAGGCTTA CATCGCACAT ACAGCCAGGA CTGCAGCTTT 
AAGAACAGCA TGTACCATGT TGGAGATTAC GTCTATGTGG AGCCCTCAGA GGTCAATCTA 
CAACCGCATA TCGTCTGTAT TGAGAGACTG TGGGAAGATT CTGCTGGTGA AAAATGGTTG 
TATGGCTGTT GGTTTTATCG GCCAAATGAA ACATTCCATT TGGCTACACG GAAATTTCTA 
GAAAAAGAAG TTTTTAAGAG TGACTATTAC AATAAAGTTC CTGTTAGTAA AATTCTAGGA 
AAATGTGTGG TCATGTTTGT CAAGGAATAC TTTAAATTAT GCCCAGAAAA TTTTCGAGAT 
GAGGATGTTT TTGTCTGTGA ATCACGATAT TCTGCCAAAA CCAAATCTTT TAAGAAAATT 
AAACTGTGGA CCATGCCCGT CAGCTCGGTT AGGTTTGTCC CTCGAGATGT GCCCTTGCCT 
GTGGTCCGAG TGGCCTCTGT ATTTGCAAAC GCAGATAAAG CGGATGATGA GAAGAATACA 
GACAACTCAG AGGACAGTCG AACTGAAGAC AGTTGTAACT TGGAAAAGGA GAAAGAAGAT 
GTCCCTGTGG AAATGTCGAA TGGTGAACCA GGTTGCCACT ACTTTGAGCA GCTCCGGTAT 
AATGACATGT GGCTGAAGGT TGGCGACTGT GTCTTCATCA AGTCCCATGG GCTAGTGCGT 
CCTCGTGTTG GCAGAATTGA AAAGGTATGG GTCCGAGATG GAGCTGCGTA TTTTTATGGC 
CCCATCTTCA TTCACCCAGA AGAGACGGAA CATGAGCCGA CAAAAATGTT CTACAAGAAG 
GAAGTGTTTT TGAGTAATCT GGAAGAAACC TGTCCCATGA CATGTATTCT AGGAAAATGT 
GCTGTGTTGT CATTCAAGGA CTTCCTCTCC TGCAGGCCAA CTGAAATTCC AGAAAATGAC 
ATTCTGCTTT GTGAGAGCCG CTACAACGAG AGTGACAAGC AGATGAAGAA ATTTAAGGGA 
CTGAAGAGAT TTTCACTGTC TGCTAAAGTG GTAGATGATG AAATTTATTA CTTCAGAAAA 
CCAATTGTTC CTCAGAAGGA GCCCTCACCT TTATTGGAAA AGAAGATCCA GTTGCTAGAA 
GCTAAATTTG CTGAGTTAGA AGGTGGAGAT GATGATATTG AAGAGATGGG AGAAGAGGAT 
AGTGAGGTCA TTGAGCCTCC CTCTCTACCT CAACTTCAGA CTCCCCTGGC CAGTGAGCTG 
GATCTCATGC CCTACACACC CCCACAGTCT ACGCCCAAAT CTGCCAAAGG CAGTGCAAAG 
AAGGAAGGCT CCAAACGGAA GATCAACATG AGTGGCTACA TCCTGTTCAG CAGTGAGATG 
AGAGCTGTGA TTAAGGCCCA GCACCCAGAC TATTCTTTTG GGGAGCTCAG CCGACTGGTG 
GGGACAGAGT GGAGAAACCT TGAGACAGCC AAGAAAGCAG AATATGAAGA GCGGGCAGCT 
AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA GCAGCACAGC AACAGCAGCC GAGTGCTTCT 
CCCCGAGCAG GCACCCCTGT GGGGGCTCTC ATGGGGGTGG TGCCACCACC AACACCAATG 
GGGATGCTCA ATCAGCAGTT GACACCTGTT GCAGGCATGA TGGGTGGCTA TCCGCCAGGC 
CTTCCACCTT TGCAGGGCCC AGTTGATGGC CTTGTTAGCA TGGGCAGCAT GCAGCCACTT 
CACCCTGGGG GGCCTCCACC CCACCATCTT CCGCCAGGTG TGCCCGGCCT CCCGGGCATC 
CCACCACCGG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCGT ATGGACAGCA GGTAGGTGTT CTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCTG GCCCACATCC TGCTGGCCCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTGGCTC CCCCACCGAA GACCCAACGG CTTCTCCACT CAGAGGCCTA CCTAAAATAC 
ATTGAAGGAC TCAGTGCTGA GTCCAACAGC ATTAGCAAGT GGGACCAGAC TCTGGCAGCT 
CGAAGACGTG ATGTCCATCT GTCGAAAGAA CAGGAGAGCC GCCTTCCCTC TCACTGGCTA 
AAAAGTAAAG GGGCCCACAC CACCATGGCA GATGCCCTAT GGCGCCTTCG GGATCTGATG 
CTCCGAGACA CCCTCAACAT TCGCCAAGCA TACAATCTAG AAAACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838262.1
CDS	137..5107
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982494.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982494.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGTAAT 
AAAAGAGCAG TCCAAGGGGG TCGTTTATCT GCAATTACCA TGGCACTTCA GTATGGCTCA 
GAAAGTGAAG AGGATGCTGC TTTAGCTGCT GCGCGATATG AAGAAGGAGA ATCTGAAGCA 
GAGAGCATTA CTTCATTTAT GGATGTTTCA AATCCTTTTT ATCAGCTTTA TGACACAGTT 
CGGAGCTGTC GGAATAACCA AGGCCAGCTA ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA 
AAGAAAAAAT ATCCTGATTA TTATCAGCAA ATTAAAACAC CCATATCACT ACAACAGATC 
AGAACAAAGC TAAAAAACCA AGAATATGAA ACTTTAGATC ATTTGGAGTG TGATCTGAAT 
TTAATGTTTG AAAATGCCAA ACGCTATAAT GTTCCCAACT CGGCCATCTA CAAGCGAGTT 
CTGAAACTGC AGCAAGTCAT GCAGGCAAAG AAGAAAGAGC TTGCTAGGAG AGACGACATT 
GAGGATGGAG ACAGCATGAT TTCTTCAGCC ACCTCTGATA CTGGTAGTGC CAAAAGAAAA 
AGTAAAAAGA ACATAAGAAA GCAGCGAATG AAAATCTTAT TCAATGTTGT TCTCGAAGCT 
CGTGAGCCAG GCTCAGGCAG AAGACTTTGT GATCTATTTA TGGTTAAACC ATCCAAAAAG 
GACTATCCTG ATTATTATAA AATCATCTTG GAGCCAATGG ACTTGAAAAT AATTGAGCAT 
AACATCCGCA ATGACAAATA TGCGGGGGAG GAGGGAATGA TAGAGGACAT GAAGCTGATG 
TTCCGGAACG CCAGGCACTA CAACGAGGAG GGCTCCCAGG TGTACAACGA TGCACACATC 
CTGGAGAAGT TACTCAAGGA TAAAAGGAAA GAGCTGGGCC CACTGCCTGA TGATGATGAC 
ATGGCATCTC CCAAACTCAA GCTGAGTAGG AAGAGTGGTA TTTCTCCTAA AAAATCAAAA 
TACATGACTC CAATGCAGCA GAAACTAAAT GAAGTCTATG AAGCTGTAAA GAACTATACT 
GATAAGAGGG GTCGCCGCCT CAGTGCCATA TTTCTGAGGC TTCCCTCCAG ATCCGAGCTG 
CCTGACTACT ATTTGACCAT TAAAAAGCCC ATGGACATGG AAAAAATTCG AAGTCACATG 
ATGGCCAACA AATACCAAGA TATAGACTCC ATGGTTGAGG ACTTCGTCAT GATGTTTAAC 
AATGCCTGTA CATACAATGA GCCCGAGTCT TTGATCTACA AAGATGCCCT TGTCCTACAC 
AAAGTGCTGC TTGAAACTCG GAGAGACTTA GAAGGAGATG AGGACTCGCA TGTCCCAAAT 
GTAACCTTGC TGATTCAGGA GCTTATCCAT AATCTTTTTG TGTCAGTCAT GAGTCATCAG 
GATGATGAAG GAAGATGCTA CAGTGATTCC TTAGCAGAAA TTCCTGCTGT GGATCCCAGC 
TTTCCAAACA AACCTCCTCT TACTTTTGAC ATAATTAGGA AGAATGTTGA AAATAACCGC 
TACCGACGGC TTGATTTATT TCAAGAGCAT ATGTTTGAAG TATTGGAGAG GGCAAGAAGG 
ATGAATCGGA CAGATTCTGA AATATATGAG GATGCGGTAG AACTTCAGCA GTTTTTTATT 
AAAATTCGTG ATGAACTCTG CAAAAATGGA GAGATCCTTC TTTCACCAGC ACTCAGCTAT 
ACCACAAAAC ATTTGCATAA TGATGTGGAG AAAGAAAAGA AGGAAAAACT GCCAAAAGAA 
ATAGAGGAAG ATAAACTAAA ACGAGAAGAA GAAAAAAGAG AAGCTGAAAA GAGTGAAGAT 
TCCTCAGGAG CTGCAGGCCT CTCAGGCTTA CATCGCACAT ACAGCCAGGA CTGCAGCTTT 
AAGAACAGCA TGTACCATGT TGGAGATTAC GTCTATGTGG AGCCCTCAGA GGTCAATCTA 
CAACCGCATA TCGTCTGTAT TGAGAGACTG TGGGAAGATT CTGCTGGTGA AAAATGGTTG 
TATGGCTGTT GGTTTTATCG GCCAAATGAA ACATTCCATT TGGCTACACG GAAATTTCTA 
GAAAAAGAAG TTTTTAAGAG TGACTATTAC AATAAAGTTC CTGTTAGTAA AATTCTAGGA 
AAATGTGTGG TCATGTTTGT CAAGGAATAC TTTAAATTAT GCCCAGAAAA TTTTCGAGAT 
GAGGATGTTT TTGTCTGTGA ATCACGATAT TCTGCCAAAA CCAAATCTTT TAAGAAAATT 
AAACTGTGGA CCATGCCCGT CAGCTCGGTT AGGTTTGTCC CTCGAGATGT GCCCTTGCCT 
GTGGTCCGAG TGGCCTCTGT ATTTGCAAAC GCAGATAAAG CGGATGATGA GAAGAATACA 
GACAACTCAG AGGACAGTCG AACTGAAGAC AGTTGTAACT TGGAAAAGGA GAAAGAAGAT 
GTCCCTGTGG AAATGTCGAA TGGTGAACCA GGTTGCCACT ACTTTGAGCA GCTCCGGTAT 
AATGACATGT GGCTGAAGGT TGGCGACTGT GTCTTCATCA AGTCCCATGG GCTAGTGCGT 
CCTCGTGTTG GCAGAATTGA AAAGGTATGG GTCCGAGATG GAGCTGCGTA TTTTTATGGC 
CCCATCTTCA TTCACCCAGA AGAGACGGAA CATGAGCCGA CAAAAATGTT CTACAAGAAG 
GAAGTGTTTT TGAGTAATCT GGAAGAAACC TGTCCCATGA CATGTATTCT AGGAAAATGT 
GCTGTGTTGT CATTCAAGGA CTTCCTCTCC TGCAGGCCAA CTGAAATTCC AGAAAATGAC 
ATTCTGCTTT GTGAGAGCCG CTACAACGAG AGTGACAAGC AGATGAAGAA ATTTAAGGGA 
CTGAAGAGAT TTTCACTGTC TGCTAAAGTG GTAGATGATG AAATTTATTA CTTCAGAAAA 
CCAATTGTTC CTCAGAAGGA GCCCTCACCT TTATTGGAAA AGAAGATCCA GTTGCTAGAA 
GCTAAATTTG CTGAGTTAGA AGGTGGAGAT GATGATATTG AAGAGATGGG AGAAGAGGAT 
AGTGAGGTCA TTGAGCCTCC CTCTCTACCT CAACTTCAGA CTCCCCTGGC CAGTGAGCTG 
GATCTCATGC CCTACACACC CCCACAGTCT ACGCCCAAAT CTGCCAAAGG CAGTGCAAAG 
AAGGAAGGCT CCAAACGGAA GATCAACATG AGTGGCTACA TCCTGTTCAG CAGTGAGATG 
AGAGCTGTGA TTAAGGCCCA GCACCCAGAC TATTCTTTTG GGGAGCTCAG CCGACTGGTG 
GGGACAGAGT GGAGAAACCT TGAGACAGCC AAGAAAGCAG AATATGAAGA GCGGGCAGCT 
AAAGTTGCTG AGCAGCAGGA GAGAGAGCGA GCAGCACAGC AACAGCAGCC GAGTGCTTCT 
CCCCGAGCAG GCACCCCTGT GGGGGCTCTC ATGGGGGTGG TGCCACCACC AACACCAATG 
GGGATGCTCA ATCAGCAGTT GACACCTGTT GCAGGCATGA TGGGTGGCTA TCCGCCAGGC 
CTTCCACCTT TGCAGGGCCC AGTTGATGGC CTTGTTAGCA TGGGCAGCAT GCAGCCACTT 
CACCCTGGGG GGCCTCCACC CCACCATCTT CCGCCAGGTG TGCCCGGCCT CCCGGGCATC 
CCACCACCGG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCGT ATGGACAGCA GGTAGGTGTT CTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCTG GCCCACATCC TGCTGGCCCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTGGCTC CCCCACCGAA GACCCAACGG CTTCTCCACT CAGAGGCCTA CCTAAAATAC 
ATTGAAGGAC TCAGTGCTGA GTCCAACAGC ATTAGCAAGT GGGACCAGAC TCTGGCAGCT 
CGAAGACGTG ATGTCCATCT GTCGAAAGAA CAGGAGAGCC GCCTTCCCTC TCACTGGCTA 
AAAAGTAAAG GGGCCCACAC CACCATGGCA GATGCCCTAT GGCGCCTTCG GGATCTGATG 
CTCCGAGACA CCCTCAACAT TCGCCAAGCA TACAATCTAG AAAACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982495.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838263.1 Bos taurus polybromo 1 (PBRM1), transcript variant X21, mRNA.

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CloneID	OBa228788
Clone ID Related Accession (Same CDS sequence)	XM_024982495.1
Accession Version	XM_024982495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCGT ATGGACAGCA GGTAGGTGTT CTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCTG GCCCACATCC TGCTGGCCCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTGGCTC CCCCACCGAA GACCCAACGG CTTCTCCACT CAGAGGCCTA CCTAAAATAC 
ATTGAAGGAC TCAGTGCTGA GTCCAACAGC ATTAGCAAGT GGGACCAGAC TCTGGCAGCT 
CGAAGACGTG ATGTCCATCT GTCGAAAGAA CAGGAGAGCC GCCTTCCCTC TCACTGGCTA 
AAAAGTAAAG GGGCCCACAC CACCATGGCA GATGCCCTAT GGCGCCTTCG GGATCTGATG 
CTCCGAGACA CCCTCAACAT TCGCCAAGCA TACAATCTAG AAAACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838263.1
CDS	79..4929
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKRAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982495.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982495.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCGT ATGGACAGCA GGTAGGTGTT CTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCTG GCCCACATCC TGCTGGCCCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTGGCTC CCCCACCGAA GACCCAACGG CTTCTCCACT CAGAGGCCTA CCTAAAATAC 
ATTGAAGGAC TCAGTGCTGA GTCCAACAGC ATTAGCAAGT GGGACCAGAC TCTGGCAGCT 
CGAAGACGTG ATGTCCATCT GTCGAAAGAA CAGGAGAGCC GCCTTCCCTC TCACTGGCTA 
AAAAGTAAAG GGGCCCACAC CACCATGGCA GATGCCCTAT GGCGCCTTCG GGATCTGATG 
CTCCGAGACA CCCTCAACAT TCGCCAAGCA TACAATCTAG AAAACCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982497.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838265.1 Bos taurus polybromo 1 (PBRM1), transcript variant X22, mRNA.

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CloneID	OBa228789
Clone ID Related Accession (Same CDS sequence)	XM_024982497.1
Accession Version	XM_024982497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838265.1
CDS	79..4923
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982497.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982497.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( NM_001305012.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001291941.1 Bos taurus polybromo 1 (PBRM1), mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	NM_001305012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	protein polybromo-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On Feb 13, 2015 this sequence version replaced XM_003587634.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145456, SAMN03145467 [ECO:0000348] ##Evidence-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291941.1
CDS	120..5231
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	NM_001305012.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305012.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_015459548.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315034.1 Bos taurus polybromo 1 (PBRM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31679
Clone ID Related Accession (Same CDS sequence)	XM_015459548.1 , XM_005222979.3 , XM_005222979.4
Accession Version	XM_015459548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315034.1
CDS	79..5253
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_015459548.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459548.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_005222979.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223036.1 Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa31679
Clone ID Related Accession (Same CDS sequence)	XM_015459548.1 , XM_005222979.3 , XM_005222979.4
Accession Version	XM_005222979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223036.1
CDS	184..5358
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_005222979.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222979.3

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_005222979.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223036.1 Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.

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CloneID	OBa31679
Clone ID Related Accession (Same CDS sequence)	XM_015459548.1 , XM_005222979.3 , XM_005222979.4
Accession Version	XM_005222979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222979.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223036.1
CDS	194..5368
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_005222979.4
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222979.4

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCCG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCGTATGGAC AGCAGGTAGG TGTTCTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTG GCTCCCCCAC CGAAGACCCA ACGGCTTCTC 
CACTCAGAGG CCTACCTAAA ATACATTGAA GGACTCAGTG CTGAGTCCAA CAGCATTAGC 
AAGTGGGACC AGACTCTGGC AGCTCGAAGA CGTGATGTCC ATCTGTCGAA AGAACAGGAG 
AGCCGCCTTC CCTCTCACTG GCTAAAAAGT AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTATGGCGCC TTCGGGATCT GATGCTCCGA GACACCCTCA ACATTCGCCA AGCATACAAT 
CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982485.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838253.1 Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.

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CloneID	OBa31681
Clone ID Related Accession (Same CDS sequence)	XM_024982485.1 , XM_010817817.2
Accession Version	XM_024982485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGAGCGGG CAGCTAAAGT TGCTGAGCAG CAGGAGAGAG AGCGAGCAGC ACAGCAACAG 
CAGCCGAGTG CTTCTCCCCG AGCAGGCACC CCTGTGGGGG CTCTCATGGG GGTGGTGCCA 
CCACCAACAC CAATGGGGAT GCTCAATCAG CAGTTGACAC CTGTTGCAGG CATGATGGGT 
GGCTATCCGC CAGGCCTTCC ACCTTTGCAG GGCCCAGTTG ATGGCCTTGT TAGCATGGGC 
AGCATGCAGC CACTTCACCC TGGGGGGCCT CCACCCCACC ATCTTCCGCC AGGTGTGCCC 
GGCCTCCCGG GCATCCCACC ACCGGGTGTG ATGAACCAAG GAGTGGCCCC TATGGTAGGG 
ACTCCAGCAC CAGGTGGAAG TCCGTATGGA CAGCAGGTAG GTGTTCTGGG GCCTCCAGGG 
CAGCAGGCAC CACCTCCATA TCCTGGCCCA CATCCTGCTG GCCCCCCTGT CATACAGCAG 
CCAACAACAC CCATGTTTGT GGCTCCCCCA CCGAAGACCC AACGGCTTCT CCACTCAGAG 
GCCTACCTAA AATACATTGA AGGACTCAGT GCTGAGTCCA ACAGCATTAG CAAGTGGGAC 
CAGACTCTGG CAGCTCGAAG ACGTGATGTC CATCTGTCGA AAGAACAGGA GAGCCGCCTT 
CCCTCTCACT GGCTAAAAAG TAAAGGGGCC CACACCACCA TGGCAGATGC CCTATGGCGC 
CTTCGGGATC TGATGCTCCG AGACACCCTC AACATTCGCC AAGCATACAA TCTAGAAAAC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838253.1
CDS	194..5359
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982485.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982485.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGAGCGGG CAGCTAAAGT TGCTGAGCAG CAGGAGAGAG AGCGAGCAGC ACAGCAACAG 
CAGCCGAGTG CTTCTCCCCG AGCAGGCACC CCTGTGGGGG CTCTCATGGG GGTGGTGCCA 
CCACCAACAC CAATGGGGAT GCTCAATCAG CAGTTGACAC CTGTTGCAGG CATGATGGGT 
GGCTATCCGC CAGGCCTTCC ACCTTTGCAG GGCCCAGTTG ATGGCCTTGT TAGCATGGGC 
AGCATGCAGC CACTTCACCC TGGGGGGCCT CCACCCCACC ATCTTCCGCC AGGTGTGCCC 
GGCCTCCCGG GCATCCCACC ACCGGGTGTG ATGAACCAAG GAGTGGCCCC TATGGTAGGG 
ACTCCAGCAC CAGGTGGAAG TCCGTATGGA CAGCAGGTAG GTGTTCTGGG GCCTCCAGGG 
CAGCAGGCAC CACCTCCATA TCCTGGCCCA CATCCTGCTG GCCCCCCTGT CATACAGCAG 
CCAACAACAC CCATGTTTGT GGCTCCCCCA CCGAAGACCC AACGGCTTCT CCACTCAGAG 
GCCTACCTAA AATACATTGA AGGACTCAGT GCTGAGTCCA ACAGCATTAG CAAGTGGGAC 
CAGACTCTGG CAGCTCGAAG ACGTGATGTC CATCTGTCGA AAGAACAGGA GAGCCGCCTT 
CCCTCTCACT GGCTAAAAAG TAAAGGGGCC CACACCACCA TGGCAGATGC CCTATGGCGC 
CTTCGGGATC TGATGCTCCG AGACACCCTC AACATTCGCC AAGCATACAA TCTAGAAAAC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817817.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816119.1 Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.

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CloneID	OBa31681
Clone ID Related Accession (Same CDS sequence)	XM_024982485.1 , XM_010817817.2
Accession Version	XM_010817817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGAGCGGG CAGCTAAAGT TGCTGAGCAG CAGGAGAGAG AGCGAGCAGC ACAGCAACAG 
CAGCCGAGTG CTTCTCCCCG AGCAGGCACC CCTGTGGGGG CTCTCATGGG GGTGGTGCCA 
CCACCAACAC CAATGGGGAT GCTCAATCAG CAGTTGACAC CTGTTGCAGG CATGATGGGT 
GGCTATCCGC CAGGCCTTCC ACCTTTGCAG GGCCCAGTTG ATGGCCTTGT TAGCATGGGC 
AGCATGCAGC CACTTCACCC TGGGGGGCCT CCACCCCACC ATCTTCCGCC AGGTGTGCCC 
GGCCTCCCGG GCATCCCACC ACCGGGTGTG ATGAACCAAG GAGTGGCCCC TATGGTAGGG 
ACTCCAGCAC CAGGTGGAAG TCCGTATGGA CAGCAGGTAG GTGTTCTGGG GCCTCCAGGG 
CAGCAGGCAC CACCTCCATA TCCTGGCCCA CATCCTGCTG GCCCCCCTGT CATACAGCAG 
CCAACAACAC CCATGTTTGT GGCTCCCCCA CCGAAGACCC AACGGCTTCT CCACTCAGAG 
GCCTACCTAA AATACATTGA AGGACTCAGT GCTGAGTCCA ACAGCATTAG CAAGTGGGAC 
CAGACTCTGG CAGCTCGAAG ACGTGATGTC CATCTGTCGA AAGAACAGGA GAGCCGCCTT 
CCCTCTCACT GGCTAAAAAG TAAAGGGGCC CACACCACCA TGGCAGATGC CCTATGGCGC 
CTTCGGGATC TGATGCTCCG AGACACCCTC AACATTCGCC AAGCATACAA TCTAGAAAAC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816119.1
CDS	79..5244
Translation	MRRLAFRGAGCALKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817817.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817817.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGAAGTTGGA TTCCATGGGT 
TCCAAGAGAA GAAGAGCTAC CTCCCCTTCC AGTAGTGTCA GTGGGGACTT TGATGATGGG 
CACCATTCTG TGTCTACACC AGGCCCAAGC AGGAAAAGGA GGAGACTTTC CAGTCTTCCA 
ACTGTAGACC CTATTGCTGT GTGCCATGAA CTCTACAACA CCATCCGAGA CTATAAGGAT 
GAGCAGGGCA GGCTCCTCTG TGAGCTCTTC ATTAGGGCAC CAAAACGAAG AAATCAACCA 
GACTATTATG AAGTGGTTTC TCAGCCCATT GACTTGATGA AAATCCAACA GAAACTAAAA 
ATGGAAGAGT ATGATGATGT TAATCTGCTT ACTGCTGACT TCCAGCTGCT TTTTAACAAT 
GCAAAGGCCT ATTATAAGCC AGATTCTCCT GAATATAAAG CTGCTTGCAA ACTCTGGGAT 
TTGTACCTTC GAACAAGAAA TGAGTTTGTT CAGAAGGGAG AAGCAGATGA CGAAGATGAT 
GATGAAGACG GGCAAGACAG TCAAGGCGCA GTGACGGAAG GATCTTCTCC AGGTTACTTG 
AAGGAGATCC TGGAGCAGCT TCTTGAAGCC ATAGTTGTAG CCACAAATCC ATCAGGACGT 
CTCATAAGCG AACTTTTTCA GAAGCTGCCT TCTAAAGTGC AATATCCAGA CTATTATGCA 
ATTATTAAGG AGCCTATAGA TCTCAAGACC ATTGCCCAGA GGATACAGAA TGGAAGCTAC 
AAGAGTATTC ACGCAATGGC CAAAGATATA GATCTCCTAG CAAAAAATGC CAAAACTTAC 
AATGAGCCTG GTTCTCAAGT GTTCAAGGAT GCAAATTCTA TTAAAAAAAT ATTTTATATG 
AAAAAGGCAG AAATTGAACA TGAAATGACA AAGTCAAGCC TTCGAATGAG GACTTCATCA 
AATTTGGCTG CAACCAGGCT GACAGGTCCT TCGCACAGTA AAGGCAGCCT TGGTGAAGAG 
AGAAATCCCA CTAGCAAGTA TTACCGTAAT AAAAGAGCAG TCCAAGGGGG TCGTTTATCT 
GCAATTACCA TGGCACTTCA GTATGGCTCA GAAAGTGAAG AGGATGCTGC TTTAGCTGCT 
GCGCGATATG AAGAAGGAGA ATCTGAAGCA GAGAGCATTA CTTCATTTAT GGATGTTTCA 
AATCCTTTTT ATCAGCTTTA TGACACAGTT CGGAGCTGTC GGAATAACCA AGGCCAGCTA 
ATAGCTGAAC CTTTTTTCCA TTTGCCTTCA AAGAAAAAAT ATCCTGATTA TTATCAGCAA 
ATTAAAACAC CCATATCACT ACAACAGATC AGAACAAAGC TAAAAAACCA AGAATATGAA 
ACTTTAGATC ATTTGGAGTG TGATCTGAAT TTAATGTTTG AAAATGCCAA ACGCTATAAT 
GTTCCCAACT CGGCCATCTA CAAGCGAGTT CTGAAACTGC AGCAAGTCAT GCAGGCAAAG 
AAGAAAGAGC TTGCTAGGAG AGACGACATT GAGGATGGAG ACAGCATGAT TTCTTCAGCC 
ACCTCTGATA CTGGTAGTGC CAAAAGAAAA AGGAGCACTC TTGACAGTGA GATGTTGGGT 
CTCAGGAGGC TCTCCAGTAA AAAGAACATA AGAAAGCAGC GAATGAAAAT CTTATTCAAT 
GTTGTTCTCG AAGCTCGTGA GCCAGGCTCA GGCAGAAGAC TTTGTGATCT ATTTATGGTT 
AAACCATCCA AAAAGGACTA TCCTGATTAT TATAAAATCA TCTTGGAGCC AATGGACTTG 
AAAATAATTG AGCATAACAT CCGCAATGAC AAATATGCGG GGGAGGAGGG AATGATAGAG 
GACATGAAGC TGATGTTCCG GAACGCCAGG CACTACAACG AGGAGGGCTC CCAGGTGTAC 
AACGATGCAC ACATCCTGGA GAAGTTACTC AAGGATAAAA GGAAAGAGCT GGGCCCACTG 
CCTGATGATG ATGACATGGC ATCTCCCAAA CTCAAGCTGA GTAGGAAGAG TGGTATTTCT 
CCTAAAAAAT CAAAATACAT GACTCCAATG CAGCAGAAAC TAAATGAAGT CTATGAAGCT 
GTAAAGAACT ATACTGATAA GAGGGGTCGC CGCCTCAGTG CCATATTTCT GAGGCTTCCC 
TCCAGATCCG AGCTGCCTGA CTACTATTTG ACCATTAAAA AGCCCATGGA CATGGAAAAA 
ATTCGAAGTC ACATGATGGC CAACAAATAC CAAGATATAG ACTCCATGGT TGAGGACTTC 
GTCATGATGT TTAACAATGC CTGTACATAC AATGAGCCCG AGTCTTTGAT CTACAAAGAT 
GCCCTTGTCC TACACAAAGT GCTGCTTGAA ACTCGGAGAG ACTTAGAAGG AGATGAGGAC 
TCGCATGTCC CAAATGTAAC CTTGCTGATT CAGGAGCTTA TCCATAATCT TTTTGTGTCA 
GTCATGAGTC ATCAGGATGA TGAAGGAAGA TGCTACAGTG ATTCCTTAGC AGAAATTCCT 
GCTGTGGATC CCAGCTTTCC AAACAAACCT CCTCTTACTT TTGACATAAT TAGGAAGAAT 
GTTGAAAATA ACCGCTACCG ACGGCTTGAT TTATTTCAAG AGCATATGTT TGAAGTATTG 
GAGAGGGCAA GAAGGATGAA TCGGACAGAT TCTGAAATAT ATGAGGATGC GGTAGAACTT 
CAGCAGTTTT TTATTAAAAT TCGTGATGAA CTCTGCAAAA ATGGAGAGAT CCTTCTTTCA 
CCAGCACTCA GCTATACCAC AAAACATTTG CATAATGATG TGGAGAAAGA AAAGAAGGAA 
AAACTGCCAA AAGAAATAGA GGAAGATAAA CTAAAACGAG AAGAAGAAAA AAGAGAAGCT 
GAAAAGAGTG AAGATTCCTC AGGAGCTGCA GGCCTCTCAG GCTTACATCG CACATACAGC 
CAGGACTGCA GCTTTAAGAA CAGCATGTAC CATGTTGGAG ATTACGTCTA TGTGGAGCCC 
TCAGAGGTCA ATCTACAACC GCATATCGTC TGTATTGAGA GACTGTGGGA AGATTCTGCT 
GGTGAAAAAT GGTTGTATGG CTGTTGGTTT TATCGGCCAA ATGAAACATT CCATTTGGCT 
ACACGGAAAT TTCTAGAAAA AGAAGTTTTT AAGAGTGACT ATTACAATAA AGTTCCTGTT 
AGTAAAATTC TAGGAAAATG TGTGGTCATG TTTGTCAAGG AATACTTTAA ATTATGCCCA 
GAAAATTTTC GAGATGAGGA TGTTTTTGTC TGTGAATCAC GATATTCTGC CAAAACCAAA 
TCTTTTAAGA AAATTAAACT GTGGACCATG CCCGTCAGCT CGGTTAGGTT TGTCCCTCGA 
GATGTGCCCT TGCCTGTGGT CCGAGTGGCC TCTGTATTTG CAAACGCAGA TAAAGCGGAT 
GATGAGAAGA ATACAGACAA CTCAGAGGAC AGTCGAACTG AAGACAGTTG TAACTTGGAA 
AAGGAGAAAG AAGATGTCCC TGTGGAAATG TCGAATGGTG AACCAGGTTG CCACTACTTT 
GAGCAGCTCC GGTATAATGA CATGTGGCTG AAGGTTGGCG ACTGTGTCTT CATCAAGTCC 
CATGGGCTAG TGCGTCCTCG TGTTGGCAGA ATTGAAAAGG TATGGGTCCG AGATGGAGCT 
GCGTATTTTT ATGGCCCCAT CTTCATTCAC CCAGAAGAGA CGGAACATGA GCCGACAAAA 
ATGTTCTACA AGAAGGAAGT GTTTTTGAGT AATCTGGAAG AAACCTGTCC CATGACATGT 
ATTCTAGGAA AATGTGCTGT GTTGTCATTC AAGGACTTCC TCTCCTGCAG GCCAACTGAA 
ATTCCAGAAA ATGACATTCT GCTTTGTGAG AGCCGCTACA ACGAGAGTGA CAAGCAGATG 
AAGAAATTTA AGGGACTGAA GAGATTTTCA CTGTCTGCTA AAGTGGTAGA TGATGAAATT 
TATTACTTCA GAAAACCAAT TGTTCCTCAG AAGGAGCCCT CACCTTTATT GGAAAAGAAG 
ATCCAGTTGC TAGAAGCTAA ATTTGCTGAG TTAGAAGGTG GAGATGATGA TATTGAAGAG 
ATGGGAGAAG AGGATAGTGA GGTCATTGAG CCTCCCTCTC TACCTCAACT TCAGACTCCC 
CTGGCCAGTG AGCTGGATCT CATGCCCTAC ACACCCCCAC AGTCTACGCC CAAATCTGCC 
AAAGGCAGTG CAAAGAAGGA AGGCTCCAAA CGGAAGATCA ACATGAGTGG CTACATCCTG 
TTCAGCAGTG AGATGAGAGC TGTGATTAAG GCCCAGCACC CAGACTATTC TTTTGGGGAG 
CTCAGCCGAC TGGTGGGGAC AGAGTGGAGA AACCTTGAGA CAGCCAAGAA AGCAGAATAT 
GAAGAGCGGG CAGCTAAAGT TGCTGAGCAG CAGGAGAGAG AGCGAGCAGC ACAGCAACAG 
CAGCCGAGTG CTTCTCCCCG AGCAGGCACC CCTGTGGGGG CTCTCATGGG GGTGGTGCCA 
CCACCAACAC CAATGGGGAT GCTCAATCAG CAGTTGACAC CTGTTGCAGG CATGATGGGT 
GGCTATCCGC CAGGCCTTCC ACCTTTGCAG GGCCCAGTTG ATGGCCTTGT TAGCATGGGC 
AGCATGCAGC CACTTCACCC TGGGGGGCCT CCACCCCACC ATCTTCCGCC AGGTGTGCCC 
GGCCTCCCGG GCATCCCACC ACCGGGTGTG ATGAACCAAG GAGTGGCCCC TATGGTAGGG 
ACTCCAGCAC CAGGTGGAAG TCCGTATGGA CAGCAGGTAG GTGTTCTGGG GCCTCCAGGG 
CAGCAGGCAC CACCTCCATA TCCTGGCCCA CATCCTGCTG GCCCCCCTGT CATACAGCAG 
CCAACAACAC CCATGTTTGT GGCTCCCCCA CCGAAGACCC AACGGCTTCT CCACTCAGAG 
GCCTACCTAA AATACATTGA AGGACTCAGT GCTGAGTCCA ACAGCATTAG CAAGTGGGAC 
CAGACTCTGG CAGCTCGAAG ACGTGATGTC CATCTGTCGA AAGAACAGGA GAGCCGCCTT 
CCCTCTCACT GGCTAAAAAG TAAAGGGGCC CACACCACCA TGGCAGATGC CCTATGGCGC 
CTTCGGGATC TGATGCTCCG AGACACCCTC AACATTCGCC AAGCATACAA TCTAGAAAAC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817819.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816121.1 Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.

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CloneID	OBa31682
Clone ID Related Accession (Same CDS sequence)	XM_010817819.3 , XM_010817819.2
Accession Version	XM_010817819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816121.1
CDS	79..5208
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817819.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817819.3

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817819.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816121.1 Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.

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CloneID	OBa31682
Clone ID Related Accession (Same CDS sequence)	XM_010817819.3 , XM_010817819.2
Accession Version	XM_010817819.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816121.1
CDS	79..5208
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817819.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817819.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_015459550.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315036.1 Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_015459550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315036.1
CDS	480..5591
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_015459550.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459550.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_005222983.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223040.1 Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_005222983.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222983.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223040.1
CDS	133..5244
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_005222983.4
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222983.4

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982487.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838255.1 Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_024982487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838255.1
CDS	263..5374
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982487.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982487.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_015459551.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315037.1 Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_015459551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315037.1
CDS	477..5588
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_015459551.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459551.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982489.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838257.1 Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_024982489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838257.1
CDS	186..5297
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982489.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982489.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_005222983.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223040.1 Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_005222983.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223040.1
CDS	133..5244
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_005222983.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222983.3

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982488.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838256.1 Bos taurus polybromo 1 (PBRM1), transcript variant X10, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_024982488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838256.1
CDS	260..5371
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982488.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982488.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982490.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838258.1 Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.

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CloneID	OBa31683
Clone ID Related Accession (Same CDS sequence)	XM_015459550.1 , XM_005222983.4 , XM_024982487.1 , XM_015459551.1 , XM_024982489.1 , XM_005222983.3 , XM_024982488.1 , NM_001305012.1 , XM_024982490.1
Accession Version	XM_024982490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838258.1
CDS	183..5294
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982490.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982490.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGTA GTGTCAGTGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC TACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAGT 
CTTCCAACTG TAGACCCTAT TGCTGTGTGC CATGAACTCT ACAACACCAT CCGAGACTAT 
AAGGATGAGC AGGGCAGGCT CCTCTGTGAG CTCTTCATTA GGGCACCAAA ACGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT CTGCTTACTG CTGACTTCCA GCTGCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCTGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AGGGAGAAGC AGATGACGAA 
GATGATGATG AAGACGGGCA AGACAGTCAA GGCGCAGTGA CGGAAGGATC TTCTCCAGGT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCCAC AAATCCATCA 
GGACGTCTCA TAAGCGAACT TTTTCAGAAG CTGCCTTCTA AAGTGCAATA TCCAGACTAT 
TATGCAATTA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAGA GTATTCACGC AATGGCCAAA GATATAGATC TCCTAGCAAA AAATGCCAAA 
ACTTACAATG AGCCTGGTTC TCAAGTGTTC AAGGATGCAA ATTCTATTAA AAAAATATTT 
TATATGAAAA AGGCAGAAAT TGAACATGAA ATGACAAAGT CAAGCCTTCG AATGAGGACT 
TCATCAAATT TGGCTGCAAC CAGGCTGACA GGTCCTTCGC ACAGTAAAGG CAGCCTTGGT 
GAAGAGAGAA ATCCCACTAG CAAGTATTAC CGTAATAAAA GAGCAGTCCA AGGGGGTCGT 
TTATCTGCAA TTACCATGGC ACTTCAGTAT GGCTCAGAAA GTGAAGAGGA TGCTGCTTTA 
GCTGCTGCGC GATATGAAGA AGGAGAATCT GAAGCAGAGA GCATTACTTC ATTTATGGAT 
GTTTCAAATC CTTTTTATCA GCTTTATGAC ACAGTTCGGA GCTGTCGGAA TAACCAAGGC 
CAGCTAATAG CTGAACCTTT TTTCCATTTG CCTTCAAAGA AAAAATATCC TGATTATTAT 
CAGCAAATTA AAACACCCAT ATCACTACAA CAGATCAGAA CAAAGCTAAA AAACCAAGAA 
TATGAAACTT TAGATCATTT GGAGTGTGAT CTGAATTTAA TGTTTGAAAA TGCCAAACGC 
TATAATGTTC CCAACTCGGC CATCTACAAG CGAGTTCTGA AACTGCAGCA AGTCATGCAG 
GCAAAGAAGA AAGAGCTTGC TAGGAGAGAC GACATTGAGG ATGGAGACAG CATGATTTCT 
TCAGCCACCT CTGATACTGG TAGTGCCAAA AGAAAAAGGA GCACTCTTGA CAGTGAGATG 
TTGGGTCTCA GGAGGCTCTC CAGTAAAAAG AACATAAGAA AGCAGCGAAT GAAAATCTTA 
TTCAATGTTG TTCTCGAAGC TCGTGAGCCA GGCTCAGGCA GAAGACTTTG TGATCTATTT 
ATGGTTAAAC CATCCAAAAA GGACTATCCT GATTATTATA AAATCATCTT GGAGCCAATG 
GACTTGAAAA TAATTGAGCA TAACATCCGC AATGACAAAT ATGCGGGGGA GGAGGGAATG 
ATAGAGGACA TGAAGCTGAT GTTCCGGAAC GCCAGGCACT ACAACGAGGA GGGCTCCCAG 
GTGTACAACG ATGCACACAT CCTGGAGAAG TTACTCAAGG ATAAAAGGAA AGAGCTGGGC 
CCACTGCCTG ATGATGATGA CATGGCATCT CCCAAACTCA AGCTGAGTAG GAAGAGTGGT 
ATTTCTCCTA AAAAATCAAA ATACATGACT CCAATGCAGC AGAAACTAAA TGAAGTCTAT 
GAAGCTGTAA AGAACTATAC TGATAAGAGG GGTCGCCGCC TCAGTGCCAT ATTTCTGAGG 
CTTCCCTCCA GATCCGAGCT GCCTGACTAC TATTTGACCA TTAAAAAGCC CATGGACATG 
GAAAAAATTC GAAGTCACAT GATGGCCAAC AAATACCAAG ATATAGACTC CATGGTTGAG 
GACTTCGTCA TGATGTTTAA CAATGCCTGT ACATACAATG AGCCCGAGTC TTTGATCTAC 
AAAGATGCCC TTGTCCTACA CAAAGTGCTG CTTGAAACTC GGAGAGACTT AGAAGGAGAT 
GAGGACTCGC ATGTCCCAAA TGTAACCTTG CTGATTCAGG AGCTTATCCA TAATCTTTTT 
GTGTCAGTCA TGAGTCATCA GGATGATGAA GGAAGATGCT ACAGTGATTC CTTAGCAGAA 
ATTCCTGCTG TGGATCCCAG CTTTCCAAAC AAACCTCCTC TTACTTTTGA CATAATTAGG 
AAGAATGTTG AAAATAACCG CTACCGACGG CTTGATTTAT TTCAAGAGCA TATGTTTGAA 
GTATTGGAGA GGGCAAGAAG GATGAATCGG ACAGATTCTG AAATATATGA GGATGCGGTA 
GAACTTCAGC AGTTTTTTAT TAAAATTCGT GATGAACTCT GCAAAAATGG AGAGATCCTT 
CTTTCACCAG CACTCAGCTA TACCACAAAA CATTTGCATA ATGATGTGGA GAAAGAAAAG 
AAGGAAAAAC TGCCAAAAGA AATAGAGGAA GATAAACTAA AACGAGAAGA AGAAAAAAGA 
GAAGCTGAAA AGAGTGAAGA TTCCTCAGGA GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGCAGCTT TAAGAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAGCCCTCAG AGGTCAATCT ACAACCGCAT ATCGTCTGTA TTGAGAGACT GTGGGAAGAT 
TCTGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTATC GGCCAAATGA AACATTCCAT 
TTGGCTACAC GGAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAATAAAGTT 
CCTGTTAGTA AAATTCTAGG AAAATGTGTG GTCATGTTTG TCAAGGAATA CTTTAAATTA 
TGCCCAGAAA ATTTTCGAGA TGAGGATGTT TTTGTCTGTG AATCACGATA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCG TCAGCTCGGT TAGGTTTGTC 
CCTCGAGATG TGCCCTTGCC TGTGGTCCGA GTGGCCTCTG TATTTGCAAA CGCAGATAAA 
GCGGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAACTGAAGA CAGTTGTAAC 
TTGGAAAAGG AGAAAGAAGA TGTCCCTGTG GAAATGTCGA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCGGTA TAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GGCTAGTGCG TCCTCGTGTT GGCAGAATTG AAAAGGTATG GGTCCGAGAT 
GGAGCTGCGT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAGACGGA ACATGAGCCG 
ACAAAAATGT TCTACAAGAA GGAAGTGTTT TTGAGTAATC TGGAAGAAAC CTGTCCCATG 
ACATGTATTC TAGGAAAATG TGCTGTGTTG TCATTCAAGG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATTC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAACGA GAGTGACAAG 
CAGATGAAGA AATTTAAGGG ACTGAAGAGA TTTTCACTGT CTGCTAAAGT GGTAGATGAT 
GAAATTTATT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCCTCACC TTTATTGGAA 
AAGAAGATCC AGTTGCTAGA AGCTAAATTT GCTGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAGGA TAGTGAGGTC ATTGAGCCTC CCTCTCTACC TCAACTTCAG 
ACTCCCCTGG CCAGTGAGCT GGATCTCATG CCCTACACAC CCCCACAGTC TACGCCCAAA 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AGATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGAGCTGTG ATTAAGGCCC AGCACCCAGA CTATTCTTTT 
GGGGAGCTCA GCCGACTGGT GGGGACAGAG TGGAGAAACC TTGAGACAGC CAAGAAAGCA 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCCGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCG TATGGACAGC AGGTAGGTGT TCTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCT GGCCCACATC CTGCTGGCCC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTGGCT CCCCCACCGA AGACCCAACG GCTTCTCCAC 
TCAGAGGCCT ACCTAAAATA CATTGAAGGA CTCAGTGCTG AGTCCAACAG CATTAGCAAG 
TGGGACCAGA CTCTGGCAGC TCGAAGACGT GATGTCCATC TGTCGAAAGA ACAGGAGAGC 
CGCCTTCCCT CTCACTGGCT AAAAAGTAAA GGGGCCCACA CCACCATGGC AGATGCCCTA 
TGGCGCCTTC GGGATCTGAT GCTCCGAGAC ACCCTCAACA TTCGCCAAGC ATACAATCTA 
GAAAACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982491.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838259.1 Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa31684
Clone ID Related Accession (Same CDS sequence)	XM_024982491.1 , XM_010817823.1
Accession Version	XM_024982491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGGAGCA CTCTTGACAG TGAGATGTTG GGTCTCAGGA GGCTCTCCAG TAAAAAGAAC 
ATAAGAAAGC AGCGAATGAA AATCTTATTC AATGTTGTTC TCGAAGCTCG TGAGCCAGGC 
TCAGGCAGAA GACTTTGTGA TCTATTTATG GTTAAACCAT CCAAAAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTGGA GCCAATGGAC TTGAAAATAA TTGAGCATAA CATCCGCAAT 
GACAAATATG CGGGGGAGGA GGGAATGATA GAGGACATGA AGCTGATGTT CCGGAACGCC 
AGGCACTACA ACGAGGAGGG CTCCCAGGTG TACAACGATG CACACATCCT GGAGAAGTTA 
CTCAAGGATA AAAGGAAAGA GCTGGGCCCA CTGCCTGATG ATGATGACAT GGCATCTCCC 
AAACTCAAGC TGAGTAGGAA GAGTGGTATT TCTCCTAAAA AATCAAAATA CATGACTCCA 
ATGCAGCAGA AACTAAATGA AGTCTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTCA GTGCCATATT TCTGAGGCTT CCCTCCAGAT CCGAGCTGCC TGACTACTAT 
TTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCCAACAAA 
TACCAAGATA TAGACTCCAT GGTTGAGGAC TTCGTCATGA TGTTTAACAA TGCCTGTACA 
TACAATGAGC CCGAGTCTTT GATCTACAAA GATGCCCTTG TCCTACACAA AGTGCTGCTT 
GAAACTCGGA GAGACTTAGA AGGAGATGAG GACTCGCATG TCCCAAATGT AACCTTGCTG 
ATTCAGGAGC TTATCCATAA TCTTTTTGTG TCAGTCATGA GTCATCAGGA TGATGAAGGA 
AGATGCTACA GTGATTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAGCTT TCCAAACAAA 
CCTCCTCTTA CTTTTGACAT AATTAGGAAG AATGTTGAAA ATAACCGCTA CCGACGGCTT 
GATTTATTTC AAGAGCATAT GTTTGAAGTA TTGGAGAGGG CAAGAAGGAT GAATCGGACA 
GATTCTGAAA TATATGAGGA TGCGGTAGAA CTTCAGCAGT TTTTTATTAA AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCACCAGCAC TCAGCTATAC CACAAAACAT 
TTGCATAATG ATGTGGAGAA AGAAAAGAAG GAAAAACTGC CAAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GAGAAGAAGA AAAAAGAGAA GCTGAAAAGA GTGAAGATTC CTCAGGAGCT 
GCAGGCCTCT CAGGCTTACA TCGCACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TACCATGTTG GAGATTACGT CTATGTGGAG CCCTCAGAGG TCAATCTACA ACCGCATATC 
GTCTGTATTG AGAGACTGTG GGAAGATTCT GCTGGTGAAA AATGGTTGTA TGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGGA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTATTACAA TAAAGTTCCT GTTAGTAAAA TTCTAGGAAA ATGTGTGGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGC CCAGAAAATT TTCGAGATGA GGATGTTTTT 
GTCTGTGAAT CACGATATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCGTCA GCTCGGTTAG GTTTGTCCCT CGAGATGTGC CCTTGCCTGT GGTCCGAGTG 
GCCTCTGTAT TTGCAAACGC AGATAAAGCG GATGATGAGA AGAATACAGA CAACTCAGAG 
GACAGTCGAA CTGAAGACAG TTGTAACTTG GAAAAGGAGA AAGAAGATGT CCCTGTGGAA 
ATGTCGAATG GTGAACCAGG TTGCCACTAC TTTGAGCAGC TCCGGTATAA TGACATGTGG 
CTGAAGGTTG GCGACTGTGT CTTCATCAAG TCCCATGGGC TAGTGCGTCC TCGTGTTGGC 
AGAATTGAAA AGGTATGGGT CCGAGATGGA GCTGCGTATT TTTATGGCCC CATCTTCATT 
CACCCAGAAG AGACGGAACA TGAGCCGACA AAAATGTTCT ACAAGAAGGA AGTGTTTTTG 
AGTAATCTGG AAGAAACCTG TCCCATGACA TGTATTCTAG GAAAATGTGC TGTGTTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATTCCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ACAACGAGAG TGACAAGCAG ATGAAGAAAT TTAAGGGACT GAAGAGATTT 
TCACTGTCTG CTAAAGTGGT AGATGATGAA ATTTATTACT TCAGAAAACC AATTGTTCCT 
CAGAAGGAGC CCTCACCTTT ATTGGAAAAG AAGATCCAGT TGCTAGAAGC TAAATTTGCT 
GAGTTAGAAG GTGGAGATGA TGATATTGAA GAGATGGGAG AAGAGGATAG TGAGGTCATT 
GAGCCTCCCT CTCTACCTCA ACTTCAGACT CCCCTGGCCA GTGAGCTGGA TCTCATGCCC 
TACACACCCC CACAGTCTAC GCCCAAATCT GCCAAAGGCA GTGCAAAGAA GGAAGGCTCC 
AAACGGAAGA TCAACATGAG TGGCTACATC CTGTTCAGCA GTGAGATGAG AGCTGTGATT 
AAGGCCCAGC ACCCAGACTA TTCTTTTGGG GAGCTCAGCC GACTGGTGGG GACAGAGTGG 
AGAAACCTTG AGACAGCCAA GAAAGCAGAA TATGAAGAGC GGGCAGCTAA AGTTGCTGAG 
CAGCAGGAGA GAGAGCGAGC AGCACAGCAA CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC 
ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG CCACCACCAA CACCAATGGG GATGCTCAAT 
CAGCAGTTGA CACCTGTTGC AGGCATGATG GGTGGCTATC CGCCAGGCCT TCCACCTTTG 
CAGGGCCCAG TTGATGGCCT TGTTAGCATG GGCAGCATGC AGCCACTTCA CCCTGGGGGG 
CCTCCACCCC ACCATCTTCC GCCAGGTGTG CCCGGCCTCC CGGGCATCCC ACCACCGGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838259.1
CDS	236..5314
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982491.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982491.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGGAGCA CTCTTGACAG TGAGATGTTG GGTCTCAGGA GGCTCTCCAG TAAAAAGAAC 
ATAAGAAAGC AGCGAATGAA AATCTTATTC AATGTTGTTC TCGAAGCTCG TGAGCCAGGC 
TCAGGCAGAA GACTTTGTGA TCTATTTATG GTTAAACCAT CCAAAAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTGGA GCCAATGGAC TTGAAAATAA TTGAGCATAA CATCCGCAAT 
GACAAATATG CGGGGGAGGA GGGAATGATA GAGGACATGA AGCTGATGTT CCGGAACGCC 
AGGCACTACA ACGAGGAGGG CTCCCAGGTG TACAACGATG CACACATCCT GGAGAAGTTA 
CTCAAGGATA AAAGGAAAGA GCTGGGCCCA CTGCCTGATG ATGATGACAT GGCATCTCCC 
AAACTCAAGC TGAGTAGGAA GAGTGGTATT TCTCCTAAAA AATCAAAATA CATGACTCCA 
ATGCAGCAGA AACTAAATGA AGTCTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTCA GTGCCATATT TCTGAGGCTT CCCTCCAGAT CCGAGCTGCC TGACTACTAT 
TTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCCAACAAA 
TACCAAGATA TAGACTCCAT GGTTGAGGAC TTCGTCATGA TGTTTAACAA TGCCTGTACA 
TACAATGAGC CCGAGTCTTT GATCTACAAA GATGCCCTTG TCCTACACAA AGTGCTGCTT 
GAAACTCGGA GAGACTTAGA AGGAGATGAG GACTCGCATG TCCCAAATGT AACCTTGCTG 
ATTCAGGAGC TTATCCATAA TCTTTTTGTG TCAGTCATGA GTCATCAGGA TGATGAAGGA 
AGATGCTACA GTGATTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAGCTT TCCAAACAAA 
CCTCCTCTTA CTTTTGACAT AATTAGGAAG AATGTTGAAA ATAACCGCTA CCGACGGCTT 
GATTTATTTC AAGAGCATAT GTTTGAAGTA TTGGAGAGGG CAAGAAGGAT GAATCGGACA 
GATTCTGAAA TATATGAGGA TGCGGTAGAA CTTCAGCAGT TTTTTATTAA AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCACCAGCAC TCAGCTATAC CACAAAACAT 
TTGCATAATG ATGTGGAGAA AGAAAAGAAG GAAAAACTGC CAAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GAGAAGAAGA AAAAAGAGAA GCTGAAAAGA GTGAAGATTC CTCAGGAGCT 
GCAGGCCTCT CAGGCTTACA TCGCACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TACCATGTTG GAGATTACGT CTATGTGGAG CCCTCAGAGG TCAATCTACA ACCGCATATC 
GTCTGTATTG AGAGACTGTG GGAAGATTCT GCTGGTGAAA AATGGTTGTA TGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGGA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTATTACAA TAAAGTTCCT GTTAGTAAAA TTCTAGGAAA ATGTGTGGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGC CCAGAAAATT TTCGAGATGA GGATGTTTTT 
GTCTGTGAAT CACGATATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCGTCA GCTCGGTTAG GTTTGTCCCT CGAGATGTGC CCTTGCCTGT GGTCCGAGTG 
GCCTCTGTAT TTGCAAACGC AGATAAAGCG GATGATGAGA AGAATACAGA CAACTCAGAG 
GACAGTCGAA CTGAAGACAG TTGTAACTTG GAAAAGGAGA AAGAAGATGT CCCTGTGGAA 
ATGTCGAATG GTGAACCAGG TTGCCACTAC TTTGAGCAGC TCCGGTATAA TGACATGTGG 
CTGAAGGTTG GCGACTGTGT CTTCATCAAG TCCCATGGGC TAGTGCGTCC TCGTGTTGGC 
AGAATTGAAA AGGTATGGGT CCGAGATGGA GCTGCGTATT TTTATGGCCC CATCTTCATT 
CACCCAGAAG AGACGGAACA TGAGCCGACA AAAATGTTCT ACAAGAAGGA AGTGTTTTTG 
AGTAATCTGG AAGAAACCTG TCCCATGACA TGTATTCTAG GAAAATGTGC TGTGTTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATTCCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ACAACGAGAG TGACAAGCAG ATGAAGAAAT TTAAGGGACT GAAGAGATTT 
TCACTGTCTG CTAAAGTGGT AGATGATGAA ATTTATTACT TCAGAAAACC AATTGTTCCT 
CAGAAGGAGC CCTCACCTTT ATTGGAAAAG AAGATCCAGT TGCTAGAAGC TAAATTTGCT 
GAGTTAGAAG GTGGAGATGA TGATATTGAA GAGATGGGAG AAGAGGATAG TGAGGTCATT 
GAGCCTCCCT CTCTACCTCA ACTTCAGACT CCCCTGGCCA GTGAGCTGGA TCTCATGCCC 
TACACACCCC CACAGTCTAC GCCCAAATCT GCCAAAGGCA GTGCAAAGAA GGAAGGCTCC 
AAACGGAAGA TCAACATGAG TGGCTACATC CTGTTCAGCA GTGAGATGAG AGCTGTGATT 
AAGGCCCAGC ACCCAGACTA TTCTTTTGGG GAGCTCAGCC GACTGGTGGG GACAGAGTGG 
AGAAACCTTG AGACAGCCAA GAAAGCAGAA TATGAAGAGC GGGCAGCTAA AGTTGCTGAG 
CAGCAGGAGA GAGAGCGAGC AGCACAGCAA CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC 
ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG CCACCACCAA CACCAATGGG GATGCTCAAT 
CAGCAGTTGA CACCTGTTGC AGGCATGATG GGTGGCTATC CGCCAGGCCT TCCACCTTTG 
CAGGGCCCAG TTGATGGCCT TGTTAGCATG GGCAGCATGC AGCCACTTCA CCCTGGGGGG 
CCTCCACCCC ACCATCTTCC GCCAGGTGTG CCCGGCCTCC CGGGCATCCC ACCACCGGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817823.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816125.1 Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa31684
Clone ID Related Accession (Same CDS sequence)	XM_024982491.1 , XM_010817823.1
Accession Version	XM_010817823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGGAGCA CTCTTGACAG TGAGATGTTG GGTCTCAGGA GGCTCTCCAG TAAAAAGAAC 
ATAAGAAAGC AGCGAATGAA AATCTTATTC AATGTTGTTC TCGAAGCTCG TGAGCCAGGC 
TCAGGCAGAA GACTTTGTGA TCTATTTATG GTTAAACCAT CCAAAAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTGGA GCCAATGGAC TTGAAAATAA TTGAGCATAA CATCCGCAAT 
GACAAATATG CGGGGGAGGA GGGAATGATA GAGGACATGA AGCTGATGTT CCGGAACGCC 
AGGCACTACA ACGAGGAGGG CTCCCAGGTG TACAACGATG CACACATCCT GGAGAAGTTA 
CTCAAGGATA AAAGGAAAGA GCTGGGCCCA CTGCCTGATG ATGATGACAT GGCATCTCCC 
AAACTCAAGC TGAGTAGGAA GAGTGGTATT TCTCCTAAAA AATCAAAATA CATGACTCCA 
ATGCAGCAGA AACTAAATGA AGTCTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTCA GTGCCATATT TCTGAGGCTT CCCTCCAGAT CCGAGCTGCC TGACTACTAT 
TTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCCAACAAA 
TACCAAGATA TAGACTCCAT GGTTGAGGAC TTCGTCATGA TGTTTAACAA TGCCTGTACA 
TACAATGAGC CCGAGTCTTT GATCTACAAA GATGCCCTTG TCCTACACAA AGTGCTGCTT 
GAAACTCGGA GAGACTTAGA AGGAGATGAG GACTCGCATG TCCCAAATGT AACCTTGCTG 
ATTCAGGAGC TTATCCATAA TCTTTTTGTG TCAGTCATGA GTCATCAGGA TGATGAAGGA 
AGATGCTACA GTGATTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAGCTT TCCAAACAAA 
CCTCCTCTTA CTTTTGACAT AATTAGGAAG AATGTTGAAA ATAACCGCTA CCGACGGCTT 
GATTTATTTC AAGAGCATAT GTTTGAAGTA TTGGAGAGGG CAAGAAGGAT GAATCGGACA 
GATTCTGAAA TATATGAGGA TGCGGTAGAA CTTCAGCAGT TTTTTATTAA AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCACCAGCAC TCAGCTATAC CACAAAACAT 
TTGCATAATG ATGTGGAGAA AGAAAAGAAG GAAAAACTGC CAAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GAGAAGAAGA AAAAAGAGAA GCTGAAAAGA GTGAAGATTC CTCAGGAGCT 
GCAGGCCTCT CAGGCTTACA TCGCACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TACCATGTTG GAGATTACGT CTATGTGGAG CCCTCAGAGG TCAATCTACA ACCGCATATC 
GTCTGTATTG AGAGACTGTG GGAAGATTCT GCTGGTGAAA AATGGTTGTA TGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGGA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTATTACAA TAAAGTTCCT GTTAGTAAAA TTCTAGGAAA ATGTGTGGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGC CCAGAAAATT TTCGAGATGA GGATGTTTTT 
GTCTGTGAAT CACGATATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCGTCA GCTCGGTTAG GTTTGTCCCT CGAGATGTGC CCTTGCCTGT GGTCCGAGTG 
GCCTCTGTAT TTGCAAACGC AGATAAAGCG GATGATGAGA AGAATACAGA CAACTCAGAG 
GACAGTCGAA CTGAAGACAG TTGTAACTTG GAAAAGGAGA AAGAAGATGT CCCTGTGGAA 
ATGTCGAATG GTGAACCAGG TTGCCACTAC TTTGAGCAGC TCCGGTATAA TGACATGTGG 
CTGAAGGTTG GCGACTGTGT CTTCATCAAG TCCCATGGGC TAGTGCGTCC TCGTGTTGGC 
AGAATTGAAA AGGTATGGGT CCGAGATGGA GCTGCGTATT TTTATGGCCC CATCTTCATT 
CACCCAGAAG AGACGGAACA TGAGCCGACA AAAATGTTCT ACAAGAAGGA AGTGTTTTTG 
AGTAATCTGG AAGAAACCTG TCCCATGACA TGTATTCTAG GAAAATGTGC TGTGTTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATTCCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ACAACGAGAG TGACAAGCAG ATGAAGAAAT TTAAGGGACT GAAGAGATTT 
TCACTGTCTG CTAAAGTGGT AGATGATGAA ATTTATTACT TCAGAAAACC AATTGTTCCT 
CAGAAGGAGC CCTCACCTTT ATTGGAAAAG AAGATCCAGT TGCTAGAAGC TAAATTTGCT 
GAGTTAGAAG GTGGAGATGA TGATATTGAA GAGATGGGAG AAGAGGATAG TGAGGTCATT 
GAGCCTCCCT CTCTACCTCA ACTTCAGACT CCCCTGGCCA GTGAGCTGGA TCTCATGCCC 
TACACACCCC CACAGTCTAC GCCCAAATCT GCCAAAGGCA GTGCAAAGAA GGAAGGCTCC 
AAACGGAAGA TCAACATGAG TGGCTACATC CTGTTCAGCA GTGAGATGAG AGCTGTGATT 
AAGGCCCAGC ACCCAGACTA TTCTTTTGGG GAGCTCAGCC GACTGGTGGG GACAGAGTGG 
AGAAACCTTG AGACAGCCAA GAAAGCAGAA TATGAAGAGC GGGCAGCTAA AGTTGCTGAG 
CAGCAGGAGA GAGAGCGAGC AGCACAGCAA CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC 
ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG CCACCACCAA CACCAATGGG GATGCTCAAT 
CAGCAGTTGA CACCTGTTGC AGGCATGATG GGTGGCTATC CGCCAGGCCT TCCACCTTTG 
CAGGGCCCAG TTGATGGCCT TGTTAGCATG GGCAGCATGC AGCCACTTCA CCCTGGGGGG 
CCTCCACCCC ACCATCTTCC GCCAGGTGTG CCCGGCCTCC CGGGCATCCC ACCACCGGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816125.1
CDS	79..5157
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817823.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817823.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGGAGCA CTCTTGACAG TGAGATGTTG GGTCTCAGGA GGCTCTCCAG TAAAAAGAAC 
ATAAGAAAGC AGCGAATGAA AATCTTATTC AATGTTGTTC TCGAAGCTCG TGAGCCAGGC 
TCAGGCAGAA GACTTTGTGA TCTATTTATG GTTAAACCAT CCAAAAAGGA CTATCCTGAT 
TATTATAAAA TCATCTTGGA GCCAATGGAC TTGAAAATAA TTGAGCATAA CATCCGCAAT 
GACAAATATG CGGGGGAGGA GGGAATGATA GAGGACATGA AGCTGATGTT CCGGAACGCC 
AGGCACTACA ACGAGGAGGG CTCCCAGGTG TACAACGATG CACACATCCT GGAGAAGTTA 
CTCAAGGATA AAAGGAAAGA GCTGGGCCCA CTGCCTGATG ATGATGACAT GGCATCTCCC 
AAACTCAAGC TGAGTAGGAA GAGTGGTATT TCTCCTAAAA AATCAAAATA CATGACTCCA 
ATGCAGCAGA AACTAAATGA AGTCTATGAA GCTGTAAAGA ACTATACTGA TAAGAGGGGT 
CGCCGCCTCA GTGCCATATT TCTGAGGCTT CCCTCCAGAT CCGAGCTGCC TGACTACTAT 
TTGACCATTA AAAAGCCCAT GGACATGGAA AAAATTCGAA GTCACATGAT GGCCAACAAA 
TACCAAGATA TAGACTCCAT GGTTGAGGAC TTCGTCATGA TGTTTAACAA TGCCTGTACA 
TACAATGAGC CCGAGTCTTT GATCTACAAA GATGCCCTTG TCCTACACAA AGTGCTGCTT 
GAAACTCGGA GAGACTTAGA AGGAGATGAG GACTCGCATG TCCCAAATGT AACCTTGCTG 
ATTCAGGAGC TTATCCATAA TCTTTTTGTG TCAGTCATGA GTCATCAGGA TGATGAAGGA 
AGATGCTACA GTGATTCCTT AGCAGAAATT CCTGCTGTGG ATCCCAGCTT TCCAAACAAA 
CCTCCTCTTA CTTTTGACAT AATTAGGAAG AATGTTGAAA ATAACCGCTA CCGACGGCTT 
GATTTATTTC AAGAGCATAT GTTTGAAGTA TTGGAGAGGG CAAGAAGGAT GAATCGGACA 
GATTCTGAAA TATATGAGGA TGCGGTAGAA CTTCAGCAGT TTTTTATTAA AATTCGTGAT 
GAACTCTGCA AAAATGGAGA GATCCTTCTT TCACCAGCAC TCAGCTATAC CACAAAACAT 
TTGCATAATG ATGTGGAGAA AGAAAAGAAG GAAAAACTGC CAAAAGAAAT AGAGGAAGAT 
AAACTAAAAC GAGAAGAAGA AAAAAGAGAA GCTGAAAAGA GTGAAGATTC CTCAGGAGCT 
GCAGGCCTCT CAGGCTTACA TCGCACATAC AGCCAGGACT GCAGCTTTAA GAACAGCATG 
TACCATGTTG GAGATTACGT CTATGTGGAG CCCTCAGAGG TCAATCTACA ACCGCATATC 
GTCTGTATTG AGAGACTGTG GGAAGATTCT GCTGGTGAAA AATGGTTGTA TGGCTGTTGG 
TTTTATCGGC CAAATGAAAC ATTCCATTTG GCTACACGGA AATTTCTAGA AAAAGAAGTT 
TTTAAGAGTG ACTATTACAA TAAAGTTCCT GTTAGTAAAA TTCTAGGAAA ATGTGTGGTC 
ATGTTTGTCA AGGAATACTT TAAATTATGC CCAGAAAATT TTCGAGATGA GGATGTTTTT 
GTCTGTGAAT CACGATATTC TGCCAAAACC AAATCTTTTA AGAAAATTAA ACTGTGGACC 
ATGCCCGTCA GCTCGGTTAG GTTTGTCCCT CGAGATGTGC CCTTGCCTGT GGTCCGAGTG 
GCCTCTGTAT TTGCAAACGC AGATAAAGCG GATGATGAGA AGAATACAGA CAACTCAGAG 
GACAGTCGAA CTGAAGACAG TTGTAACTTG GAAAAGGAGA AAGAAGATGT CCCTGTGGAA 
ATGTCGAATG GTGAACCAGG TTGCCACTAC TTTGAGCAGC TCCGGTATAA TGACATGTGG 
CTGAAGGTTG GCGACTGTGT CTTCATCAAG TCCCATGGGC TAGTGCGTCC TCGTGTTGGC 
AGAATTGAAA AGGTATGGGT CCGAGATGGA GCTGCGTATT TTTATGGCCC CATCTTCATT 
CACCCAGAAG AGACGGAACA TGAGCCGACA AAAATGTTCT ACAAGAAGGA AGTGTTTTTG 
AGTAATCTGG AAGAAACCTG TCCCATGACA TGTATTCTAG GAAAATGTGC TGTGTTGTCA 
TTCAAGGACT TCCTCTCCTG CAGGCCAACT GAAATTCCAG AAAATGACAT TCTGCTTTGT 
GAGAGCCGCT ACAACGAGAG TGACAAGCAG ATGAAGAAAT TTAAGGGACT GAAGAGATTT 
TCACTGTCTG CTAAAGTGGT AGATGATGAA ATTTATTACT TCAGAAAACC AATTGTTCCT 
CAGAAGGAGC CCTCACCTTT ATTGGAAAAG AAGATCCAGT TGCTAGAAGC TAAATTTGCT 
GAGTTAGAAG GTGGAGATGA TGATATTGAA GAGATGGGAG AAGAGGATAG TGAGGTCATT 
GAGCCTCCCT CTCTACCTCA ACTTCAGACT CCCCTGGCCA GTGAGCTGGA TCTCATGCCC 
TACACACCCC CACAGTCTAC GCCCAAATCT GCCAAAGGCA GTGCAAAGAA GGAAGGCTCC 
AAACGGAAGA TCAACATGAG TGGCTACATC CTGTTCAGCA GTGAGATGAG AGCTGTGATT 
AAGGCCCAGC ACCCAGACTA TTCTTTTGGG GAGCTCAGCC GACTGGTGGG GACAGAGTGG 
AGAAACCTTG AGACAGCCAA GAAAGCAGAA TATGAAGAGC GGGCAGCTAA AGTTGCTGAG 
CAGCAGGAGA GAGAGCGAGC AGCACAGCAA CAGCAGCCGA GTGCTTCTCC CCGAGCAGGC 
ACCCCTGTGG GGGCTCTCAT GGGGGTGGTG CCACCACCAA CACCAATGGG GATGCTCAAT 
CAGCAGTTGA CACCTGTTGC AGGCATGATG GGTGGCTATC CGCCAGGCCT TCCACCTTTG 
CAGGGCCCAG TTGATGGCCT TGTTAGCATG GGCAGCATGC AGCCACTTCA CCCTGGGGGG 
CCTCCACCCC ACCATCTTCC GCCAGGTGTG CCCGGCCTCC CGGGCATCCC ACCACCGGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817824.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816126.1 Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.

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CloneID	OBa31685
Clone ID Related Accession (Same CDS sequence)	XM_010817824.2
Accession Version	XM_010817824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816126.1
CDS	79..5097
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817824.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817824.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCGTAT 
GGACAGCAGG TAGGTGTTCT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCTGGC 
CCACATCCTG CTGGCCCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTGGCTCCC 
CCACCGAAGA CCCAACGGCT TCTCCACTCA GAGGCCTACC TAAAATACAT TGAAGGACTC 
AGTGCTGAGT CCAACAGCAT TAGCAAGTGG GACCAGACTC TGGCAGCTCG AAGACGTGAT 
GTCCATCTGT CGAAAGAACA GGAGAGCCGC CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTATGG CGCCTTCGGG ATCTGATGCT CCGAGACACC 
CTCAACATTC GCCAAGCATA CAATCTAGAA AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817826.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816128.1 Bos taurus polybromo 1 (PBRM1), transcript variant X16, mRNA.

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CloneID	OBa31686
Clone ID Related Accession (Same CDS sequence)	XM_010817826.1
Accession Version	XM_010817826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816128.1
CDS	79..5088
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817826.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817826.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCCGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCGTA TGGACAGCAG 
GTAGGTGTTC TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCTGG CCCACATCCT 
GCTGGCCCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTGGCTCC CCCACCGAAG 
ACCCAACGGC TTCTCCACTC AGAGGCCTAC CTAAAATACA TTGAAGGACT CAGTGCTGAG 
TCCAACAGCA TTAGCAAGTG GGACCAGACT CTGGCAGCTC GAAGACGTGA TGTCCATCTG 
TCGAAAGAAC AGGAGAGCCG CCTTCCCTCT CACTGGCTAA AAAGTAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTATG GCGCCTTCGG GATCTGATGC TCCGAGACAC CCTCAACATT 
CGCCAAGCAT ACAATCTAGA AAACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817827.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816129.1 Bos taurus polybromo 1 (PBRM1), transcript variant X18, mRNA.

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CloneID	OBa31687
Clone ID Related Accession (Same CDS sequence)	XM_010817827.3 , XM_010817827.2
Accession Version	XM_010817827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816129.1
CDS	79..5043
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817827.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817827.3

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817827.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816129.1 Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.

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CloneID	OBa31687
Clone ID Related Accession (Same CDS sequence)	XM_010817827.3 , XM_010817827.2
Accession Version	XM_010817827.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816129.1
CDS	79..5043
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817827.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817827.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCCG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCGTATGGAC AGCAGGTAGG TGTTCTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCTGGCCCAC ATCCTGCTGG CCCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTG 
GCTCCCCCAC CGAAGACCCA ACGGCTTCTC CACTCAGAGG CCTACCTAAA ATACATTGAA 
GGACTCAGTG CTGAGTCCAA CAGCATTAGC AAGTGGGACC AGACTCTGGC AGCTCGAAGA 
CGTGATGTCC ATCTGTCGAA AGAACAGGAG AGCCGCCTTC CCTCTCACTG GCTAAAAAGT 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTATGGCGCC TTCGGGATCT GATGCTCCGA 
GACACCCTCA ACATTCGCCA AGCATACAAT CTAGAAAACC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817828.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816130.1 Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa31688
Clone ID Related Accession (Same CDS sequence)	XM_010817828.2 , XM_010817828.3
Accession Version	XM_010817828.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGTAAAA AGAACATAAG AAAGCAGCGA ATGAAAATCT TATTCAATGT TGTTCTCGAA 
GCTCGTGAGC CAGGCTCAGG CAGAAGACTT TGTGATCTAT TTATGGTTAA ACCATCCAAA 
AAGGACTATC CTGATTATTA TAAAATCATC TTGGAGCCAA TGGACTTGAA AATAATTGAG 
CATAACATCC GCAATGACAA ATATGCGGGG GAGGAGGGAA TGATAGAGGA CATGAAGCTG 
ATGTTCCGGA ACGCCAGGCA CTACAACGAG GAGGGCTCCC AGGTGTACAA CGATGCACAC 
ATCCTGGAGA AGTTACTCAA GGATAAAAGG AAAGAGCTGG GCCCACTGCC TGATGATGAT 
GACATGGCAT CTCCCAAACT CAAGCTGAGT AGGAAGAGTG GTATTTCTCC TAAAAAATCA 
AAATACATGA CTCCAATGCA GCAGAAACTA AATGAAGTCT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTCAGTGCC ATATTTCTGA GGCTTCCCTC CAGATCCGAG 
CTGCCTGACT ACTATTTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCCA ACAAATACCA AGATATAGAC TCCATGGTTG AGGACTTCGT CATGATGTTT 
AACAATGCCT GTACATACAA TGAGCCCGAG TCTTTGATCT ACAAAGATGC CCTTGTCCTA 
CACAAAGTGC TGCTTGAAAC TCGGAGAGAC TTAGAAGGAG ATGAGGACTC GCATGTCCCA 
AATGTAACCT TGCTGATTCA GGAGCTTATC CATAATCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGATG AAGGAAGATG CTACAGTGAT TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AGCTTTCCAA ACAAACCTCC TCTTACTTTT GACATAATTA GGAAGAATGT TGAAAATAAC 
CGCTACCGAC GGCTTGATTT ATTTCAAGAG CATATGTTTG AAGTATTGGA GAGGGCAAGA 
AGGATGAATC GGACAGATTC TGAAATATAT GAGGATGCGG TAGAACTTCA GCAGTTTTTT 
ATTAAAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCACC AGCACTCAGC 
TATACCACAA AACATTTGCA TAATGATGTG GAGAAAGAAA AGAAGGAAAA ACTGCCAAAA 
GAAATAGAGG AAGATAAACT AAAACGAGAA GAAGAAAAAA GAGAAGCTGA AAAGAGTGAA 
GATTCCTCAG GAGCTGCAGG CCTCTCAGGC TTACATCGCA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTACCA TGTTGGAGAT TACGTCTATG TGGAGCCCTC AGAGGTCAAT 
CTACAACCGC ATATCGTCTG TATTGAGAGA CTGTGGGAAG ATTCTGCTGG TGAAAAATGG 
TTGTATGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGGAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAT TACAATAAAG TTCCTGTTAG TAAAATTCTA 
GGAAAATGTG TGGTCATGTT TGTCAAGGAA TACTTTAAAT TATGCCCAGA AAATTTTCGA 
GATGAGGATG TTTTTGTCTG TGAATCACGA TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CGTCAGCTCG GTTAGGTTTG TCCCTCGAGA TGTGCCCTTG 
CCTGTGGTCC GAGTGGCCTC TGTATTTGCA AACGCAGATA AAGCGGATGA TGAGAAGAAT 
ACAGACAACT CAGAGGACAG TCGAACTGAA GACAGTTGTA ACTTGGAAAA GGAGAAAGAA 
GATGTCCCTG TGGAAATGTC GAATGGTGAA CCAGGTTGCC ACTACTTTGA GCAGCTCCGG 
TATAATGACA TGTGGCTGAA GGTTGGCGAC TGTGTCTTCA TCAAGTCCCA TGGGCTAGTG 
CGTCCTCGTG TTGGCAGAAT TGAAAAGGTA TGGGTCCGAG ATGGAGCTGC GTATTTTTAT 
GGCCCCATCT TCATTCACCC AGAAGAGACG GAACATGAGC CGACAAAAAT GTTCTACAAG 
AAGGAAGTGT TTTTGAGTAA TCTGGAAGAA ACCTGTCCCA TGACATGTAT TCTAGGAAAA 
TGTGCTGTGT TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT TCCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTACAAC GAGAGTGACA AGCAGATGAA GAAATTTAAG 
GGACTGAAGA GATTTTCACT GTCTGCTAAA GTGGTAGATG ATGAAATTTA TTACTTCAGA 
AAACCAATTG TTCCTCAGAA GGAGCCCTCA CCTTTATTGG AAAAGAAGAT CCAGTTGCTA 
GAAGCTAAAT TTGCTGAGTT AGAAGGTGGA GATGATGATA TTGAAGAGAT GGGAGAAGAG 
GATAGTGAGG TCATTGAGCC TCCCTCTCTA CCTCAACTTC AGACTCCCCT GGCCAGTGAG 
CTGGATCTCA TGCCCTACAC ACCCCCACAG TCTACGCCCA AATCTGCCAA AGGCAGTGCA 
AAGAAGGAAG GCTCCAAACG GAAGATCAAC ATGAGTGGCT ACATCCTGTT CAGCAGTGAG 
ATGAGAGCTG TGATTAAGGC CCAGCACCCA GACTATTCTT TTGGGGAGCT CAGCCGACTG 
GTGGGGACAG AGTGGAGAAA CCTTGAGACA GCCAAGAAAG CAGAATATGA AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCCC ACCATCTTCC GCCAGGTGTG 
CCCGGCCTCC CGGGCATCCC ACCACCGGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816130.1
CDS	79..4947
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817828.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817828.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGTAAAA AGAACATAAG AAAGCAGCGA ATGAAAATCT TATTCAATGT TGTTCTCGAA 
GCTCGTGAGC CAGGCTCAGG CAGAAGACTT TGTGATCTAT TTATGGTTAA ACCATCCAAA 
AAGGACTATC CTGATTATTA TAAAATCATC TTGGAGCCAA TGGACTTGAA AATAATTGAG 
CATAACATCC GCAATGACAA ATATGCGGGG GAGGAGGGAA TGATAGAGGA CATGAAGCTG 
ATGTTCCGGA ACGCCAGGCA CTACAACGAG GAGGGCTCCC AGGTGTACAA CGATGCACAC 
ATCCTGGAGA AGTTACTCAA GGATAAAAGG AAAGAGCTGG GCCCACTGCC TGATGATGAT 
GACATGGCAT CTCCCAAACT CAAGCTGAGT AGGAAGAGTG GTATTTCTCC TAAAAAATCA 
AAATACATGA CTCCAATGCA GCAGAAACTA AATGAAGTCT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTCAGTGCC ATATTTCTGA GGCTTCCCTC CAGATCCGAG 
CTGCCTGACT ACTATTTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCCA ACAAATACCA AGATATAGAC TCCATGGTTG AGGACTTCGT CATGATGTTT 
AACAATGCCT GTACATACAA TGAGCCCGAG TCTTTGATCT ACAAAGATGC CCTTGTCCTA 
CACAAAGTGC TGCTTGAAAC TCGGAGAGAC TTAGAAGGAG ATGAGGACTC GCATGTCCCA 
AATGTAACCT TGCTGATTCA GGAGCTTATC CATAATCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGATG AAGGAAGATG CTACAGTGAT TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AGCTTTCCAA ACAAACCTCC TCTTACTTTT GACATAATTA GGAAGAATGT TGAAAATAAC 
CGCTACCGAC GGCTTGATTT ATTTCAAGAG CATATGTTTG AAGTATTGGA GAGGGCAAGA 
AGGATGAATC GGACAGATTC TGAAATATAT GAGGATGCGG TAGAACTTCA GCAGTTTTTT 
ATTAAAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCACC AGCACTCAGC 
TATACCACAA AACATTTGCA TAATGATGTG GAGAAAGAAA AGAAGGAAAA ACTGCCAAAA 
GAAATAGAGG AAGATAAACT AAAACGAGAA GAAGAAAAAA GAGAAGCTGA AAAGAGTGAA 
GATTCCTCAG GAGCTGCAGG CCTCTCAGGC TTACATCGCA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTACCA TGTTGGAGAT TACGTCTATG TGGAGCCCTC AGAGGTCAAT 
CTACAACCGC ATATCGTCTG TATTGAGAGA CTGTGGGAAG ATTCTGCTGG TGAAAAATGG 
TTGTATGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGGAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAT TACAATAAAG TTCCTGTTAG TAAAATTCTA 
GGAAAATGTG TGGTCATGTT TGTCAAGGAA TACTTTAAAT TATGCCCAGA AAATTTTCGA 
GATGAGGATG TTTTTGTCTG TGAATCACGA TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CGTCAGCTCG GTTAGGTTTG TCCCTCGAGA TGTGCCCTTG 
CCTGTGGTCC GAGTGGCCTC TGTATTTGCA AACGCAGATA AAGCGGATGA TGAGAAGAAT 
ACAGACAACT CAGAGGACAG TCGAACTGAA GACAGTTGTA ACTTGGAAAA GGAGAAAGAA 
GATGTCCCTG TGGAAATGTC GAATGGTGAA CCAGGTTGCC ACTACTTTGA GCAGCTCCGG 
TATAATGACA TGTGGCTGAA GGTTGGCGAC TGTGTCTTCA TCAAGTCCCA TGGGCTAGTG 
CGTCCTCGTG TTGGCAGAAT TGAAAAGGTA TGGGTCCGAG ATGGAGCTGC GTATTTTTAT 
GGCCCCATCT TCATTCACCC AGAAGAGACG GAACATGAGC CGACAAAAAT GTTCTACAAG 
AAGGAAGTGT TTTTGAGTAA TCTGGAAGAA ACCTGTCCCA TGACATGTAT TCTAGGAAAA 
TGTGCTGTGT TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT TCCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTACAAC GAGAGTGACA AGCAGATGAA GAAATTTAAG 
GGACTGAAGA GATTTTCACT GTCTGCTAAA GTGGTAGATG ATGAAATTTA TTACTTCAGA 
AAACCAATTG TTCCTCAGAA GGAGCCCTCA CCTTTATTGG AAAAGAAGAT CCAGTTGCTA 
GAAGCTAAAT TTGCTGAGTT AGAAGGTGGA GATGATGATA TTGAAGAGAT GGGAGAAGAG 
GATAGTGAGG TCATTGAGCC TCCCTCTCTA CCTCAACTTC AGACTCCCCT GGCCAGTGAG 
CTGGATCTCA TGCCCTACAC ACCCCCACAG TCTACGCCCA AATCTGCCAA AGGCAGTGCA 
AAGAAGGAAG GCTCCAAACG GAAGATCAAC ATGAGTGGCT ACATCCTGTT CAGCAGTGAG 
ATGAGAGCTG TGATTAAGGC CCAGCACCCA GACTATTCTT TTGGGGAGCT CAGCCGACTG 
GTGGGGACAG AGTGGAGAAA CCTTGAGACA GCCAAGAAAG CAGAATATGA AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCCC ACCATCTTCC GCCAGGTGTG 
CCCGGCCTCC CGGGCATCCC ACCACCGGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817828.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816130.1 Bos taurus polybromo 1 (PBRM1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa31688
Clone ID Related Accession (Same CDS sequence)	XM_010817828.2 , XM_010817828.3
Accession Version	XM_010817828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGTAAAA AGAACATAAG AAAGCAGCGA ATGAAAATCT TATTCAATGT TGTTCTCGAA 
GCTCGTGAGC CAGGCTCAGG CAGAAGACTT TGTGATCTAT TTATGGTTAA ACCATCCAAA 
AAGGACTATC CTGATTATTA TAAAATCATC TTGGAGCCAA TGGACTTGAA AATAATTGAG 
CATAACATCC GCAATGACAA ATATGCGGGG GAGGAGGGAA TGATAGAGGA CATGAAGCTG 
ATGTTCCGGA ACGCCAGGCA CTACAACGAG GAGGGCTCCC AGGTGTACAA CGATGCACAC 
ATCCTGGAGA AGTTACTCAA GGATAAAAGG AAAGAGCTGG GCCCACTGCC TGATGATGAT 
GACATGGCAT CTCCCAAACT CAAGCTGAGT AGGAAGAGTG GTATTTCTCC TAAAAAATCA 
AAATACATGA CTCCAATGCA GCAGAAACTA AATGAAGTCT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTCAGTGCC ATATTTCTGA GGCTTCCCTC CAGATCCGAG 
CTGCCTGACT ACTATTTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCCA ACAAATACCA AGATATAGAC TCCATGGTTG AGGACTTCGT CATGATGTTT 
AACAATGCCT GTACATACAA TGAGCCCGAG TCTTTGATCT ACAAAGATGC CCTTGTCCTA 
CACAAAGTGC TGCTTGAAAC TCGGAGAGAC TTAGAAGGAG ATGAGGACTC GCATGTCCCA 
AATGTAACCT TGCTGATTCA GGAGCTTATC CATAATCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGATG AAGGAAGATG CTACAGTGAT TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AGCTTTCCAA ACAAACCTCC TCTTACTTTT GACATAATTA GGAAGAATGT TGAAAATAAC 
CGCTACCGAC GGCTTGATTT ATTTCAAGAG CATATGTTTG AAGTATTGGA GAGGGCAAGA 
AGGATGAATC GGACAGATTC TGAAATATAT GAGGATGCGG TAGAACTTCA GCAGTTTTTT 
ATTAAAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCACC AGCACTCAGC 
TATACCACAA AACATTTGCA TAATGATGTG GAGAAAGAAA AGAAGGAAAA ACTGCCAAAA 
GAAATAGAGG AAGATAAACT AAAACGAGAA GAAGAAAAAA GAGAAGCTGA AAAGAGTGAA 
GATTCCTCAG GAGCTGCAGG CCTCTCAGGC TTACATCGCA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTACCA TGTTGGAGAT TACGTCTATG TGGAGCCCTC AGAGGTCAAT 
CTACAACCGC ATATCGTCTG TATTGAGAGA CTGTGGGAAG ATTCTGCTGG TGAAAAATGG 
TTGTATGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGGAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAT TACAATAAAG TTCCTGTTAG TAAAATTCTA 
GGAAAATGTG TGGTCATGTT TGTCAAGGAA TACTTTAAAT TATGCCCAGA AAATTTTCGA 
GATGAGGATG TTTTTGTCTG TGAATCACGA TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CGTCAGCTCG GTTAGGTTTG TCCCTCGAGA TGTGCCCTTG 
CCTGTGGTCC GAGTGGCCTC TGTATTTGCA AACGCAGATA AAGCGGATGA TGAGAAGAAT 
ACAGACAACT CAGAGGACAG TCGAACTGAA GACAGTTGTA ACTTGGAAAA GGAGAAAGAA 
GATGTCCCTG TGGAAATGTC GAATGGTGAA CCAGGTTGCC ACTACTTTGA GCAGCTCCGG 
TATAATGACA TGTGGCTGAA GGTTGGCGAC TGTGTCTTCA TCAAGTCCCA TGGGCTAGTG 
CGTCCTCGTG TTGGCAGAAT TGAAAAGGTA TGGGTCCGAG ATGGAGCTGC GTATTTTTAT 
GGCCCCATCT TCATTCACCC AGAAGAGACG GAACATGAGC CGACAAAAAT GTTCTACAAG 
AAGGAAGTGT TTTTGAGTAA TCTGGAAGAA ACCTGTCCCA TGACATGTAT TCTAGGAAAA 
TGTGCTGTGT TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT TCCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTACAAC GAGAGTGACA AGCAGATGAA GAAATTTAAG 
GGACTGAAGA GATTTTCACT GTCTGCTAAA GTGGTAGATG ATGAAATTTA TTACTTCAGA 
AAACCAATTG TTCCTCAGAA GGAGCCCTCA CCTTTATTGG AAAAGAAGAT CCAGTTGCTA 
GAAGCTAAAT TTGCTGAGTT AGAAGGTGGA GATGATGATA TTGAAGAGAT GGGAGAAGAG 
GATAGTGAGG TCATTGAGCC TCCCTCTCTA CCTCAACTTC AGACTCCCCT GGCCAGTGAG 
CTGGATCTCA TGCCCTACAC ACCCCCACAG TCTACGCCCA AATCTGCCAA AGGCAGTGCA 
AAGAAGGAAG GCTCCAAACG GAAGATCAAC ATGAGTGGCT ACATCCTGTT CAGCAGTGAG 
ATGAGAGCTG TGATTAAGGC CCAGCACCCA GACTATTCTT TTGGGGAGCT CAGCCGACTG 
GTGGGGACAG AGTGGAGAAA CCTTGAGACA GCCAAGAAAG CAGAATATGA AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCCC ACCATCTTCC GCCAGGTGTG 
CCCGGCCTCC CGGGCATCCC ACCACCGGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816130.1
CDS	79..4947
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMSNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817828.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817828.3

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGT 
AATAAAAGAG CAGTCCAAGG GGGTCGTTTA TCTGCAATTA CCATGGCACT TCAGTATGGC 
TCAGAAAGTG AAGAGGATGC TGCTTTAGCT GCTGCGCGAT ATGAAGAAGG AGAATCTGAA 
GCAGAGAGCA TTACTTCATT TATGGATGTT TCAAATCCTT TTTATCAGCT TTATGACACA 
GTTCGGAGCT GTCGGAATAA CCAAGGCCAG CTAATAGCTG AACCTTTTTT CCATTTGCCT 
TCAAAGAAAA AATATCCTGA TTATTATCAG CAAATTAAAA CACCCATATC ACTACAACAG 
ATCAGAACAA AGCTAAAAAA CCAAGAATAT GAAACTTTAG ATCATTTGGA GTGTGATCTG 
AATTTAATGT TTGAAAATGC CAAACGCTAT AATGTTCCCA ACTCGGCCAT CTACAAGCGA 
GTTCTGAAAC TGCAGCAAGT CATGCAGGCA AAGAAGAAAG AGCTTGCTAG GAGAGACGAC 
ATTGAGGATG GAGACAGCAT GATTTCTTCA GCCACCTCTG ATACTGGTAG TGCCAAAAGA 
AAAAGTAAAA AGAACATAAG AAAGCAGCGA ATGAAAATCT TATTCAATGT TGTTCTCGAA 
GCTCGTGAGC CAGGCTCAGG CAGAAGACTT TGTGATCTAT TTATGGTTAA ACCATCCAAA 
AAGGACTATC CTGATTATTA TAAAATCATC TTGGAGCCAA TGGACTTGAA AATAATTGAG 
CATAACATCC GCAATGACAA ATATGCGGGG GAGGAGGGAA TGATAGAGGA CATGAAGCTG 
ATGTTCCGGA ACGCCAGGCA CTACAACGAG GAGGGCTCCC AGGTGTACAA CGATGCACAC 
ATCCTGGAGA AGTTACTCAA GGATAAAAGG AAAGAGCTGG GCCCACTGCC TGATGATGAT 
GACATGGCAT CTCCCAAACT CAAGCTGAGT AGGAAGAGTG GTATTTCTCC TAAAAAATCA 
AAATACATGA CTCCAATGCA GCAGAAACTA AATGAAGTCT ATGAAGCTGT AAAGAACTAT 
ACTGATAAGA GGGGTCGCCG CCTCAGTGCC ATATTTCTGA GGCTTCCCTC CAGATCCGAG 
CTGCCTGACT ACTATTTGAC CATTAAAAAG CCCATGGACA TGGAAAAAAT TCGAAGTCAC 
ATGATGGCCA ACAAATACCA AGATATAGAC TCCATGGTTG AGGACTTCGT CATGATGTTT 
AACAATGCCT GTACATACAA TGAGCCCGAG TCTTTGATCT ACAAAGATGC CCTTGTCCTA 
CACAAAGTGC TGCTTGAAAC TCGGAGAGAC TTAGAAGGAG ATGAGGACTC GCATGTCCCA 
AATGTAACCT TGCTGATTCA GGAGCTTATC CATAATCTTT TTGTGTCAGT CATGAGTCAT 
CAGGATGATG AAGGAAGATG CTACAGTGAT TCCTTAGCAG AAATTCCTGC TGTGGATCCC 
AGCTTTCCAA ACAAACCTCC TCTTACTTTT GACATAATTA GGAAGAATGT TGAAAATAAC 
CGCTACCGAC GGCTTGATTT ATTTCAAGAG CATATGTTTG AAGTATTGGA GAGGGCAAGA 
AGGATGAATC GGACAGATTC TGAAATATAT GAGGATGCGG TAGAACTTCA GCAGTTTTTT 
ATTAAAATTC GTGATGAACT CTGCAAAAAT GGAGAGATCC TTCTTTCACC AGCACTCAGC 
TATACCACAA AACATTTGCA TAATGATGTG GAGAAAGAAA AGAAGGAAAA ACTGCCAAAA 
GAAATAGAGG AAGATAAACT AAAACGAGAA GAAGAAAAAA GAGAAGCTGA AAAGAGTGAA 
GATTCCTCAG GAGCTGCAGG CCTCTCAGGC TTACATCGCA CATACAGCCA GGACTGCAGC 
TTTAAGAACA GCATGTACCA TGTTGGAGAT TACGTCTATG TGGAGCCCTC AGAGGTCAAT 
CTACAACCGC ATATCGTCTG TATTGAGAGA CTGTGGGAAG ATTCTGCTGG TGAAAAATGG 
TTGTATGGCT GTTGGTTTTA TCGGCCAAAT GAAACATTCC ATTTGGCTAC ACGGAAATTT 
CTAGAAAAAG AAGTTTTTAA GAGTGACTAT TACAATAAAG TTCCTGTTAG TAAAATTCTA 
GGAAAATGTG TGGTCATGTT TGTCAAGGAA TACTTTAAAT TATGCCCAGA AAATTTTCGA 
GATGAGGATG TTTTTGTCTG TGAATCACGA TATTCTGCCA AAACCAAATC TTTTAAGAAA 
ATTAAACTGT GGACCATGCC CGTCAGCTCG GTTAGGTTTG TCCCTCGAGA TGTGCCCTTG 
CCTGTGGTCC GAGTGGCCTC TGTATTTGCA AACGCAGATA AAGCGGATGA TGAGAAGAAT 
ACAGACAACT CAGAGGACAG TCGAACTGAA GACAGTTGTA ACTTGGAAAA GGAGAAAGAA 
GATGTCCCTG TGGAAATGTC GAATGGTGAA CCAGGTTGCC ACTACTTTGA GCAGCTCCGG 
TATAATGACA TGTGGCTGAA GGTTGGCGAC TGTGTCTTCA TCAAGTCCCA TGGGCTAGTG 
CGTCCTCGTG TTGGCAGAAT TGAAAAGGTA TGGGTCCGAG ATGGAGCTGC GTATTTTTAT 
GGCCCCATCT TCATTCACCC AGAAGAGACG GAACATGAGC CGACAAAAAT GTTCTACAAG 
AAGGAAGTGT TTTTGAGTAA TCTGGAAGAA ACCTGTCCCA TGACATGTAT TCTAGGAAAA 
TGTGCTGTGT TGTCATTCAA GGACTTCCTC TCCTGCAGGC CAACTGAAAT TCCAGAAAAT 
GACATTCTGC TTTGTGAGAG CCGCTACAAC GAGAGTGACA AGCAGATGAA GAAATTTAAG 
GGACTGAAGA GATTTTCACT GTCTGCTAAA GTGGTAGATG ATGAAATTTA TTACTTCAGA 
AAACCAATTG TTCCTCAGAA GGAGCCCTCA CCTTTATTGG AAAAGAAGAT CCAGTTGCTA 
GAAGCTAAAT TTGCTGAGTT AGAAGGTGGA GATGATGATA TTGAAGAGAT GGGAGAAGAG 
GATAGTGAGG TCATTGAGCC TCCCTCTCTA CCTCAACTTC AGACTCCCCT GGCCAGTGAG 
CTGGATCTCA TGCCCTACAC ACCCCCACAG TCTACGCCCA AATCTGCCAA AGGCAGTGCA 
AAGAAGGAAG GCTCCAAACG GAAGATCAAC ATGAGTGGCT ACATCCTGTT CAGCAGTGAG 
ATGAGAGCTG TGATTAAGGC CCAGCACCCA GACTATTCTT TTGGGGAGCT CAGCCGACTG 
GTGGGGACAG AGTGGAGAAA CCTTGAGACA GCCAAGAAAG CAGAATATGA AGGCATGATG 
GGTGGCTATC CGCCAGGCCT TCCACCTTTG CAGGGCCCAG TTGATGGCCT TGTTAGCATG 
GGCAGCATGC AGCCACTTCA CCCTGGGGGG CCTCCACCCC ACCATCTTCC GCCAGGTGTG 
CCCGGCCTCC CGGGCATCCC ACCACCGGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817829.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816131.1 Bos taurus polybromo 1 (PBRM1), transcript variant X20, mRNA.

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CloneID	OBa31689
Clone ID Related Accession (Same CDS sequence)	XM_010817829.1
Accession Version	XM_010817829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA GCAGGTAGGT GTTCTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGCCCACA TCCTGCTGGC CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTGG CTCCCCCACC GAAGACCCAA CGGCTTCTCC ACTCAGAGGC CTACCTAAAA 
TACATTGAAG GACTCAGTGC TGAGTCCAAC AGCATTAGCA AGTGGGACCA GACTCTGGCA 
GCTCGAAGAC GTGATGTCCA TCTGTCGAAA GAACAGGAGA GCCGCCTTCC CTCTCACTGG 
CTAAAAAGTA AAGGGGCCCA CACCACCATG GCAGATGCCC TATGGCGCCT TCGGGATCTG 
ATGCTCCGAG ACACCCTCAA CATTCGCCAA GCATACAATC TAGAAAACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816131.1
CDS	79..4932
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817829.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817829.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAGCACTCT TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT CTCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTCGA AGCTCGTGAG CCAGGCTCAG GCAGAAGACT TTGTGATCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCGGG GGAGGAGGGA 
ATGATAGAGG ACATGAAGCT GATGTTCCGG AACGCCAGGC ACTACAACGA GGAGGGCTCC 
CAGGTGTACA ACGATGCACA CATCCTGGAG AAGTTACTCA AGGATAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCA TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGTATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAAGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CCAGATCCGA GCTGCCTGAC TACTATTTGA CCATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAATACC AAGATATAGA CTCCATGGTT 
GAGGACTTCG TCATGATGTT TAACAATGCC TGTACATACA ATGAGCCCGA GTCTTTGATC 
TACAAAGATG CCCTTGTCCT ACACAAAGTG CTGCTTGAAA CTCGGAGAGA CTTAGAAGGA 
GATGAGGACT CGCATGTCCC AAATGTAACC TTGCTGATTC AGGAGCTTAT CCATAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAAGGAAGAT GCTACAGTGA TTCCTTAGCA 
GAAATTCCTG CTGTGGATCC CAGCTTTCCA AACAAACCTC CTCTTACTTT TGACATAATT 
AGGAAGAATG TTGAAAATAA CCGCTACCGA CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AGAGGGCAAG AAGGATGAAT CGGACAGATT CTGAAATATA TGAGGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATC 
CTTCTTTCAC CAGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAA 
AAGAAGGAAA AACTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCA GGAGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGCAG CTTTAAGAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAGCCCT CAGAGGTCAA TCTACAACCG CATATCGTCT GTATTGAGAG ACTGTGGGAA 
GATTCTGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ATCGGCCAAA TGAAACATTC 
CATTTGGCTA CACGGAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAATAAA 
GTTCCTGTTA GTAAAATTCT AGGAAAATGT GTGGTCATGT TTGTCAAGGA ATACTTTAAA 
TTATGCCCAG AAAATTTTCG AGATGAGGAT GTTTTTGTCT GTGAATCACG ATATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCGTCAGCTC GGTTAGGTTT 
GTCCCTCGAG ATGTGCCCTT GCCTGTGGTC CGAGTGGCCT CTGTATTTGC AAACGCAGAT 
AAAGCGGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAACTGA AGACAGTTGT 
AACTTGGAAA AGGAGAAAGA AGATGTCCCT GTGGAAATGT CGAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCG GTATAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGGCTAGT GCGTCCTCGT GTTGGCAGAA TTGAAAAGGT ATGGGTCCGA 
GATGGAGCTG CGTATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAGAC GGAACATGAG 
CCGACAAAAA TGTTCTACAA GAAGGAAGTG TTTTTGAGTA ATCTGGAAGA AACCTGTCCC 
ATGACATGTA TTCTAGGAAA ATGTGCTGTG TTGTCATTCA AGGACTTCCT CTCCTGCAGG 
CCAACTGAAA TTCCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA CGAGAGTGAC 
AAGCAGATGA AGAAATTTAA GGGACTGAAG AGATTTTCAC TGTCTGCTAA AGTGGTAGAT 
GATGAAATTT ATTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCCTC ACCTTTATTG 
GAAAAGAAGA TCCAGTTGCT AGAAGCTAAA TTTGCTGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA GGATAGTGAG GTCATTGAGC CTCCCTCTCT ACCTCAACTT 
CAGACTCCCC TGGCCAGTGA GCTGGATCTC ATGCCCTACA CACCCCCACA GTCTACGCCC 
AAATCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAGATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGAGCT GTGATTAAGG CCCAGCACCC AGACTATTCT 
TTTGGGGAGC TCAGCCGACT GGTGGGGACA GAGTGGAGAA ACCTTGAGAC AGCCAAGAAA 
GCAGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CGTATGGACA GCAGGTAGGT GTTCTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CTGGCCCACA TCCTGCTGGC CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTGG CTCCCCCACC GAAGACCCAA CGGCTTCTCC ACTCAGAGGC CTACCTAAAA 
TACATTGAAG GACTCAGTGC TGAGTCCAAC AGCATTAGCA AGTGGGACCA GACTCTGGCA 
GCTCGAAGAC GTGATGTCCA TCTGTCGAAA GAACAGGAGA GCCGCCTTCC CTCTCACTGG 
CTAAAAAGTA AAGGGGCCCA CACCACCATG GCAGATGCCC TATGGCGCCT TCGGGATCTG 
ATGCTCCGAG ACACCCTCAA CATTCGCCAA GCATACAATC TAGAAAACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817830.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816132.1 Bos taurus polybromo 1 (PBRM1), transcript variant X23, mRNA.

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CloneID	OBa31690
Clone ID Related Accession (Same CDS sequence)	XM_010817830.1
Accession Version	XM_010817830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816132.1
CDS	79..4887
Translation	MRRLAFRGAGCALVKLKKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817830.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817830.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGG TAAAGCTGAA GAAGTTGGAT 
TCCATGGGTT CCAAGAGAAG AAGAGCTACC TCCCCTTCCA GTAGTGTCAG TGGGGACTTT 
GATGATGGGC ACCATTCTGT GTCTACACCA GGCCCAAGCA GGAAAAGGAG GAGACTTTCC 
AGTCTTCCAA CTGTAGACCC TATTGCTGTG TGCCATGAAC TCTACAACAC CATCCGAGAC 
TATAAGGATG AGCAGGGCAG GCTCCTCTGT GAGCTCTTCA TTAGGGCACC AAAACGAAGA 
AATCAACCAG ACTATTATGA AGTGGTTTCT CAGCCCATTG ACTTGATGAA AATCCAACAG 
AAACTAAAAA TGGAAGAGTA TGATGATGTT AATCTGCTTA CTGCTGACTT CCAGCTGCTT 
TTTAACAATG CAAAGGCCTA TTATAAGCCA GATTCTCCTG AATATAAAGC TGCTTGCAAA 
CTCTGGGATT TGTACCTTCG AACAAGAAAT GAGTTTGTTC AGAAGGGAGA AGCAGATGAC 
GAAGATGATG ATGAAGACGG GCAAGACAGT CAAGGCGCAG TGACGGAAGG ATCTTCTCCA 
GGTTACTTGA AGGAGATCCT GGAGCAGCTT CTTGAAGCCA TAGTTGTAGC CACAAATCCA 
TCAGGACGTC TCATAAGCGA ACTTTTTCAG AAGCTGCCTT CTAAAGTGCA ATATCCAGAC 
TATTATGCAA TTATTAAGGA GCCTATAGAT CTCAAGACCA TTGCCCAGAG GATACAGAAT 
GGAAGCTACA AGAGTATTCA CGCAATGGCC AAAGATATAG ATCTCCTAGC AAAAAATGCC 
AAAACTTACA ATGAGCCTGG TTCTCAAGTG TTCAAGGATG CAAATTCTAT TAAAAAAATA 
TTTTATATGA AAAAGGCAGA AATTGAACAT GAAATGACAA AGTCAAGCCT TCGAATGAGG 
ACTTCATCAA ATTTGGCTGC AACCAGGCTG ACAGGTCCTT CGCACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT CCAAGGGGGT 
CGTTTATCTG CAATTACCAT GGCACTTCAG TATGGCTCAG AAAGTGAAGA GGATGCTGCT 
TTAGCTGCTG CGCGATATGA AGAAGGAGAA TCTGAAGCAG AGAGCATTAC TTCATTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTC GGAGCTGTCG GAATAACCAA 
GGCCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA TCCTGATTAT 
TATCAGCAAA TTAAAACACC CATATCACTA CAACAGATCA GAACAAAGCT AAAAAACCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TTCCCAACTC GGCCATCTAC AAGCGAGTTC TGAAACTGCA GCAAGTCATG 
CAGGCAAAGA AGAAAGAGCT TGCTAGGAGA GACGACATTG AGGATGGAGA CAGCATGATT 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTCGAAGCTC GTGAGCCAGG CTCAGGCAGA 
AGACTTTGTG ATCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCGGGGGAGG AGGGAATGAT AGAGGACATG AAGCTGATGT TCCGGAACGC CAGGCACTAC 
AACGAGGAGG GCTCCCAGGT GTACAACGAT GCACACATCC TGGAGAAGTT ACTCAAGGAT 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCATCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGTAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AAGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCCAGA TCCGAGCTGC CTGACTACTA TTTGACCATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA ATACCAAGAT 
ATAGACTCCA TGGTTGAGGA CTTCGTCATG ATGTTTAACA ATGCCTGTAC ATACAATGAG 
CCCGAGTCTT TGATCTACAA AGATGCCCTT GTCCTACACA AAGTGCTGCT TGAAACTCGG 
AGAGACTTAG AAGGAGATGA GGACTCGCAT GTCCCAAATG TAACCTTGCT GATTCAGGAG 
CTTATCCATA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAAGG AAGATGCTAC 
AGTGATTCCT TAGCAGAAAT TCCTGCTGTG GATCCCAGCT TTCCAAACAA ACCTCCTCTT 
ACTTTTGACA TAATTAGGAA GAATGTTGAA AATAACCGCT ACCGACGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAGAGG GCAAGAAGGA TGAATCGGAC AGATTCTGAA 
ATATATGAGG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATCCTTCT TTCACCAGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAAAAGAA GGAAAAACTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCAGGAGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGCAGCTTTA AGAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA GCCCTCAGAG GTCAATCTAC AACCGCATAT CGTCTGTATT 
GAGAGACTGT GGGAAGATTC TGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTATCGG 
CCAAATGAAA CATTCCATTT GGCTACACGG AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ATAAAGTTCC TGTTAGTAAA ATTCTAGGAA AATGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAATTATG CCCAGAAAAT TTTCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGATATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCGTC 
AGCTCGGTTA GGTTTGTCCC TCGAGATGTG CCCTTGCCTG TGGTCCGAGT GGCCTCTGTA 
TTTGCAAACG CAGATAAAGC GGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
ACTGAAGACA GTTGTAACTT GGAAAAGGAG AAAGAAGATG TCCCTGTGGA AATGTCGAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCGGTATA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGG CTAGTGCGTC CTCGTGTTGG CAGAATTGAA 
AAGGTATGGG TCCGAGATGG AGCTGCGTAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAGACGGAAC ATGAGCCGAC AAAAATGTTC TACAAGAAGG AAGTGTTTTT GAGTAATCTG 
GAAGAAACCT GTCCCATGAC ATGTATTCTA GGAAAATGTG CTGTGTTGTC ATTCAAGGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATTCCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAACGAGA GTGACAAGCA GATGAAGAAA TTTAAGGGAC TGAAGAGATT TTCACTGTCT 
GCTAAAGTGG TAGATGATGA AATTTATTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCCTCACCTT TATTGGAAAA GAAGATCCAG TTGCTAGAAG CTAAATTTGC TGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAGGATA GTGAGGTCAT TGAGCCTCCC 
TCTCTACCTC AACTTCAGAC TCCCCTGGCC AGTGAGCTGG ATCTCATGCC CTACACACCC 
CCACAGTCTA CGCCCAAATC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAG 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GAGCTGTGAT TAAGGCCCAG 
CACCCAGACT ATTCTTTTGG GGAGCTCAGC CGACTGGTGG GGACAGAGTG GAGAAACCTT 
GAGACAGCCA AGAAAGCAGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCGTAT GGACAGCAGG TAGGTGTTCT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCTGGC CCACATCCTG CTGGCCCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTGGCTCCC CCACCGAAGA CCCAACGGCT TCTCCACTCA 
GAGGCCTACC TAAAATACAT TGAAGGACTC AGTGCTGAGT CCAACAGCAT TAGCAAGTGG 
GACCAGACTC TGGCAGCTCG AAGACGTGAT GTCCATCTGT CGAAAGAACA GGAGAGCCGC 
CTTCCCTCTC ACTGGCTAAA AAGTAAAGGG GCCCACACCA CCATGGCAGA TGCCCTATGG 
CGCCTTCGGG ATCTGATGCT CCGAGACACC CTCAACATTC GCCAAGCATA CAATCTAGAA 
AACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024982486.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838254.1 Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.

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CloneID	OBa46598
Clone ID Related Accession (Same CDS sequence)	XM_024982486.1 , XM_010817818.2
Accession Version	XM_024982486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCTACCTC CCCTTCCAGT AGTGTCAGTG GGGACTTTGA TGATGGGCAC 
CATTCTGTGT CTACACCAGG CCCAAGCAGG AAAAGGAGGA GACTTTCCAG TCTTCCAACT 
GTAGACCCTA TTGCTGTGTG CCATGAACTC TACAACACCA TCCGAGACTA TAAGGATGAG 
CAGGGCAGGC TCCTCTGTGA GCTCTTCATT AGGGCACCAA AACGAAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTAAAAATG 
GAAGAGTATG ATGATGTTAA TCTGCTTACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTATT ATAAGCCAGA TTCTCCTGAA TATAAAGCTG CTTGCAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAGGGAGAAG CAGATGACGA AGATGATGAT 
GAAGACGGGC AAGACAGTCA AGGCGCAGTG ACGGAAGGAT CTTCTCCAGG TTACTTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGTCTC 
ATAAGCGAAC TTTTTCAGAA GCTGCCTTCT AAAGTGCAAT ATCCAGACTA TTATGCAATT 
ATTAAGGAGC CTATAGATCT CAAGACCATT GCCCAGAGGA TACAGAATGG AAGCTACAAG 
AGTATTCACG CAATGGCCAA AGATATAGAT CTCCTAGCAA AAAATGCCAA AACTTACAAT 
GAGCCTGGTT CTCAAGTGTT CAAGGATGCA AATTCTATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATGA AATGACAAAG TCAAGCCTTC GAATGAGGAC TTCATCAAAT 
TTGGCTGCAA CCAGGCTGAC AGGTCCTTCG CACAGTAAAG GCAGCCTTGG TGAAGAGAGA 
AATCCCACTA GCAAGTATTA CCGTAATAAA AGAGCAGTCC AAGGGGGTCG TTTATCTGCA 
ATTACCATGG CACTTCAGTA TGGCTCAGAA AGTGAAGAGG ATGCTGCTTT AGCTGCTGCG 
CGATATGAAG AAGGAGAATC TGAAGCAGAG AGCATTACTT CATTTATGGA TGTTTCAAAT 
CCTTTTTATC AGCTTTATGA CACAGTTCGG AGCTGTCGGA ATAACCAAGG CCAGCTAATA 
GCTGAACCTT TTTTCCATTT GCCTTCAAAG AAAAAATATC CTGATTATTA TCAGCAAATT 
AAAACACCCA TATCACTACA ACAGATCAGA ACAAAGCTAA AAAACCAAGA ATATGAAACT 
TTAGATCATT TGGAGTGTGA TCTGAATTTA ATGTTTGAAA ATGCCAAACG CTATAATGTT 
CCCAACTCGG CCATCTACAA GCGAGTTCTG AAACTGCAGC AAGTCATGCA GGCAAAGAAG 
AAAGAGCTTG CTAGGAGAGA CGACATTGAG GATGGAGACA GCATGATTTC TTCAGCCACC 
TCTGATACTG GTAGTGCCAA AAGAAAAAGG AGCACTCTTG ACAGTGAGAT GTTGGGTCTC 
AGGAGGCTCT CCAGTAAAAA GAACATAAGA AAGCAGCGAA TGAAAATCTT ATTCAATGTT 
GTTCTCGAAG CTCGTGAGCC AGGCTCAGGC AGAAGACTTT GTGATCTATT TATGGTTAAA 
CCATCCAAAA AGGACTATCC TGATTATTAT AAAATCATCT TGGAGCCAAT GGACTTGAAA 
ATAATTGAGC ATAACATCCG CAATGACAAA TATGCGGGGG AGGAGGGAAT GATAGAGGAC 
ATGAAGCTGA TGTTCCGGAA CGCCAGGCAC TACAACGAGG AGGGCTCCCA GGTGTACAAC 
GATGCACACA TCCTGGAGAA GTTACTCAAG GATAAAAGGA AAGAGCTGGG CCCACTGCCT 
GATGATGATG ACATGGCATC TCCCAAACTC AAGCTGAGTA GGAAGAGTGG TATTTCTCCT 
AAAAAATCAA AATACATGAC TCCAATGCAG CAGAAACTAA ATGAAGTCTA TGAAGCTGTA 
AAGAACTATA CTGATAAGAG GGGTCGCCGC CTCAGTGCCA TATTTCTGAG GCTTCCCTCC 
AGATCCGAGC TGCCTGACTA CTATTTGACC ATTAAAAAGC CCATGGACAT GGAAAAAATT 
CGAAGTCACA TGATGGCCAA CAAATACCAA GATATAGACT CCATGGTTGA GGACTTCGTC 
ATGATGTTTA ACAATGCCTG TACATACAAT GAGCCCGAGT CTTTGATCTA CAAAGATGCC 
CTTGTCCTAC ACAAAGTGCT GCTTGAAACT CGGAGAGACT TAGAAGGAGA TGAGGACTCG 
CATGTCCCAA ATGTAACCTT GCTGATTCAG GAGCTTATCC ATAATCTTTT TGTGTCAGTC 
ATGAGTCATC AGGATGATGA AGGAAGATGC TACAGTGATT CCTTAGCAGA AATTCCTGCT 
GTGGATCCCA GCTTTCCAAA CAAACCTCCT CTTACTTTTG ACATAATTAG GAAGAATGTT 
GAAAATAACC GCTACCGACG GCTTGATTTA TTTCAAGAGC ATATGTTTGA AGTATTGGAG 
AGGGCAAGAA GGATGAATCG GACAGATTCT GAAATATATG AGGATGCGGT AGAACTTCAG 
CAGTTTTTTA TTAAAATTCG TGATGAACTC TGCAAAAATG GAGAGATCCT TCTTTCACCA 
GCACTCAGCT ATACCACAAA ACATTTGCAT AATGATGTGG AGAAAGAAAA GAAGGAAAAA 
CTGCCAAAAG AAATAGAGGA AGATAAACTA AAACGAGAAG AAGAAAAAAG AGAAGCTGAA 
AAGAGTGAAG ATTCCTCAGG AGCTGCAGGC CTCTCAGGCT TACATCGCAC ATACAGCCAG 
GACTGCAGCT TTAAGAACAG CATGTACCAT GTTGGAGATT ACGTCTATGT GGAGCCCTCA 
GAGGTCAATC TACAACCGCA TATCGTCTGT ATTGAGAGAC TGTGGGAAGA TTCTGCTGGT 
GAAAAATGGT TGTATGGCTG TTGGTTTTAT CGGCCAAATG AAACATTCCA TTTGGCTACA 
CGGAAATTTC TAGAAAAAGA AGTTTTTAAG AGTGACTATT ACAATAAAGT TCCTGTTAGT 
AAAATTCTAG GAAAATGTGT GGTCATGTTT GTCAAGGAAT ACTTTAAATT ATGCCCAGAA 
AATTTTCGAG ATGAGGATGT TTTTGTCTGT GAATCACGAT ATTCTGCCAA AACCAAATCT 
TTTAAGAAAA TTAAACTGTG GACCATGCCC GTCAGCTCGG TTAGGTTTGT CCCTCGAGAT 
GTGCCCTTGC CTGTGGTCCG AGTGGCCTCT GTATTTGCAA ACGCAGATAA AGCGGATGAT 
GAGAAGAATA CAGACAACTC AGAGGACAGT CGAACTGAAG ACAGTTGTAA CTTGGAAAAG 
GAGAAAGAAG ATGTCCCTGT GGAAATGTCG AATGGTGAAC CAGGTTGCCA CTACTTTGAG 
CAGCTCCGGT ATAATGACAT GTGGCTGAAG GTTGGCGACT GTGTCTTCAT CAAGTCCCAT 
GGGCTAGTGC GTCCTCGTGT TGGCAGAATT GAAAAGGTAT GGGTCCGAGA TGGAGCTGCG 
TATTTTTATG GCCCCATCTT CATTCACCCA GAAGAGACGG AACATGAGCC GACAAAAATG 
TTCTACAAGA AGGAAGTGTT TTTGAGTAAT CTGGAAGAAA CCTGTCCCAT GACATGTATT 
CTAGGAAAAT GTGCTGTGTT GTCATTCAAG GACTTCCTCT CCTGCAGGCC AACTGAAATT 
CCAGAAAATG ACATTCTGCT TTGTGAGAGC CGCTACAACG AGAGTGACAA GCAGATGAAG 
AAATTTAAGG GACTGAAGAG ATTTTCACTG TCTGCTAAAG TGGTAGATGA TGAAATTTAT 
TACTTCAGAA AACCAATTGT TCCTCAGAAG GAGCCCTCAC CTTTATTGGA AAAGAAGATC 
CAGTTGCTAG AAGCTAAATT TGCTGAGTTA GAAGGTGGAG ATGATGATAT TGAAGAGATG 
GGAGAAGAGG ATAGTGAGGT CATTGAGCCT CCCTCTCTAC CTCAACTTCA GACTCCCCTG 
GCCAGTGAGC TGGATCTCAT GCCCTACACA CCCCCACAGT CTACGCCCAA ATCTGCCAAA 
GGCAGTGCAA AGAAGGAAGG CTCCAAACGG AAGATCAACA TGAGTGGCTA CATCCTGTTC 
AGCAGTGAGA TGAGAGCTGT GATTAAGGCC CAGCACCCAG ACTATTCTTT TGGGGAGCTC 
AGCCGACTGG TGGGGACAGA GTGGAGAAAC CTTGAGACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCCCACCATC TTCCGCCAGG TGTGCCCGGC 
CTCCCGGGCA TCCCACCACC GGGTGTGATG AACCAAGGAG TGGCCCCTAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAG CAGGTAGGTG TTCTGGGGCC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGCCCACAT CCTGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACACCCA TGTTTGTGGC TCCCCCACCG AAGACCCAAC GGCTTCTCCA CTCAGAGGCC 
TACCTAAAAT ACATTGAAGG ACTCAGTGCT GAGTCCAACA GCATTAGCAA GTGGGACCAG 
ACTCTGGCAG CTCGAAGACG TGATGTCCAT CTGTCGAAAG AACAGGAGAG CCGCCTTCCC 
TCTCACTGGC TAAAAAGTAA AGGGGCCCAC ACCACCATGG CAGATGCCCT ATGGCGCCTT 
CGGGATCTGA TGCTCCGAGA CACCCTCAAC ATTCGCCAAG CATACAATCT AGAAAACCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838254.1
CDS	194..5356
Translation	MRRLAFRGAGCALKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_024982486.1
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982486.1

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCTACCTC CCCTTCCAGT AGTGTCAGTG GGGACTTTGA TGATGGGCAC 
CATTCTGTGT CTACACCAGG CCCAAGCAGG AAAAGGAGGA GACTTTCCAG TCTTCCAACT 
GTAGACCCTA TTGCTGTGTG CCATGAACTC TACAACACCA TCCGAGACTA TAAGGATGAG 
CAGGGCAGGC TCCTCTGTGA GCTCTTCATT AGGGCACCAA AACGAAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTAAAAATG 
GAAGAGTATG ATGATGTTAA TCTGCTTACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTATT ATAAGCCAGA TTCTCCTGAA TATAAAGCTG CTTGCAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAGGGAGAAG CAGATGACGA AGATGATGAT 
GAAGACGGGC AAGACAGTCA AGGCGCAGTG ACGGAAGGAT CTTCTCCAGG TTACTTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGTCTC 
ATAAGCGAAC TTTTTCAGAA GCTGCCTTCT AAAGTGCAAT ATCCAGACTA TTATGCAATT 
ATTAAGGAGC CTATAGATCT CAAGACCATT GCCCAGAGGA TACAGAATGG AAGCTACAAG 
AGTATTCACG CAATGGCCAA AGATATAGAT CTCCTAGCAA AAAATGCCAA AACTTACAAT 
GAGCCTGGTT CTCAAGTGTT CAAGGATGCA AATTCTATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATGA AATGACAAAG TCAAGCCTTC GAATGAGGAC TTCATCAAAT 
TTGGCTGCAA CCAGGCTGAC AGGTCCTTCG CACAGTAAAG GCAGCCTTGG TGAAGAGAGA 
AATCCCACTA GCAAGTATTA CCGTAATAAA AGAGCAGTCC AAGGGGGTCG TTTATCTGCA 
ATTACCATGG CACTTCAGTA TGGCTCAGAA AGTGAAGAGG ATGCTGCTTT AGCTGCTGCG 
CGATATGAAG AAGGAGAATC TGAAGCAGAG AGCATTACTT CATTTATGGA TGTTTCAAAT 
CCTTTTTATC AGCTTTATGA CACAGTTCGG AGCTGTCGGA ATAACCAAGG CCAGCTAATA 
GCTGAACCTT TTTTCCATTT GCCTTCAAAG AAAAAATATC CTGATTATTA TCAGCAAATT 
AAAACACCCA TATCACTACA ACAGATCAGA ACAAAGCTAA AAAACCAAGA ATATGAAACT 
TTAGATCATT TGGAGTGTGA TCTGAATTTA ATGTTTGAAA ATGCCAAACG CTATAATGTT 
CCCAACTCGG CCATCTACAA GCGAGTTCTG AAACTGCAGC AAGTCATGCA GGCAAAGAAG 
AAAGAGCTTG CTAGGAGAGA CGACATTGAG GATGGAGACA GCATGATTTC TTCAGCCACC 
TCTGATACTG GTAGTGCCAA AAGAAAAAGG AGCACTCTTG ACAGTGAGAT GTTGGGTCTC 
AGGAGGCTCT CCAGTAAAAA GAACATAAGA AAGCAGCGAA TGAAAATCTT ATTCAATGTT 
GTTCTCGAAG CTCGTGAGCC AGGCTCAGGC AGAAGACTTT GTGATCTATT TATGGTTAAA 
CCATCCAAAA AGGACTATCC TGATTATTAT AAAATCATCT TGGAGCCAAT GGACTTGAAA 
ATAATTGAGC ATAACATCCG CAATGACAAA TATGCGGGGG AGGAGGGAAT GATAGAGGAC 
ATGAAGCTGA TGTTCCGGAA CGCCAGGCAC TACAACGAGG AGGGCTCCCA GGTGTACAAC 
GATGCACACA TCCTGGAGAA GTTACTCAAG GATAAAAGGA AAGAGCTGGG CCCACTGCCT 
GATGATGATG ACATGGCATC TCCCAAACTC AAGCTGAGTA GGAAGAGTGG TATTTCTCCT 
AAAAAATCAA AATACATGAC TCCAATGCAG CAGAAACTAA ATGAAGTCTA TGAAGCTGTA 
AAGAACTATA CTGATAAGAG GGGTCGCCGC CTCAGTGCCA TATTTCTGAG GCTTCCCTCC 
AGATCCGAGC TGCCTGACTA CTATTTGACC ATTAAAAAGC CCATGGACAT GGAAAAAATT 
CGAAGTCACA TGATGGCCAA CAAATACCAA GATATAGACT CCATGGTTGA GGACTTCGTC 
ATGATGTTTA ACAATGCCTG TACATACAAT GAGCCCGAGT CTTTGATCTA CAAAGATGCC 
CTTGTCCTAC ACAAAGTGCT GCTTGAAACT CGGAGAGACT TAGAAGGAGA TGAGGACTCG 
CATGTCCCAA ATGTAACCTT GCTGATTCAG GAGCTTATCC ATAATCTTTT TGTGTCAGTC 
ATGAGTCATC AGGATGATGA AGGAAGATGC TACAGTGATT CCTTAGCAGA AATTCCTGCT 
GTGGATCCCA GCTTTCCAAA CAAACCTCCT CTTACTTTTG ACATAATTAG GAAGAATGTT 
GAAAATAACC GCTACCGACG GCTTGATTTA TTTCAAGAGC ATATGTTTGA AGTATTGGAG 
AGGGCAAGAA GGATGAATCG GACAGATTCT GAAATATATG AGGATGCGGT AGAACTTCAG 
CAGTTTTTTA TTAAAATTCG TGATGAACTC TGCAAAAATG GAGAGATCCT TCTTTCACCA 
GCACTCAGCT ATACCACAAA ACATTTGCAT AATGATGTGG AGAAAGAAAA GAAGGAAAAA 
CTGCCAAAAG AAATAGAGGA AGATAAACTA AAACGAGAAG AAGAAAAAAG AGAAGCTGAA 
AAGAGTGAAG ATTCCTCAGG AGCTGCAGGC CTCTCAGGCT TACATCGCAC ATACAGCCAG 
GACTGCAGCT TTAAGAACAG CATGTACCAT GTTGGAGATT ACGTCTATGT GGAGCCCTCA 
GAGGTCAATC TACAACCGCA TATCGTCTGT ATTGAGAGAC TGTGGGAAGA TTCTGCTGGT 
GAAAAATGGT TGTATGGCTG TTGGTTTTAT CGGCCAAATG AAACATTCCA TTTGGCTACA 
CGGAAATTTC TAGAAAAAGA AGTTTTTAAG AGTGACTATT ACAATAAAGT TCCTGTTAGT 
AAAATTCTAG GAAAATGTGT GGTCATGTTT GTCAAGGAAT ACTTTAAATT ATGCCCAGAA 
AATTTTCGAG ATGAGGATGT TTTTGTCTGT GAATCACGAT ATTCTGCCAA AACCAAATCT 
TTTAAGAAAA TTAAACTGTG GACCATGCCC GTCAGCTCGG TTAGGTTTGT CCCTCGAGAT 
GTGCCCTTGC CTGTGGTCCG AGTGGCCTCT GTATTTGCAA ACGCAGATAA AGCGGATGAT 
GAGAAGAATA CAGACAACTC AGAGGACAGT CGAACTGAAG ACAGTTGTAA CTTGGAAAAG 
GAGAAAGAAG ATGTCCCTGT GGAAATGTCG AATGGTGAAC CAGGTTGCCA CTACTTTGAG 
CAGCTCCGGT ATAATGACAT GTGGCTGAAG GTTGGCGACT GTGTCTTCAT CAAGTCCCAT 
GGGCTAGTGC GTCCTCGTGT TGGCAGAATT GAAAAGGTAT GGGTCCGAGA TGGAGCTGCG 
TATTTTTATG GCCCCATCTT CATTCACCCA GAAGAGACGG AACATGAGCC GACAAAAATG 
TTCTACAAGA AGGAAGTGTT TTTGAGTAAT CTGGAAGAAA CCTGTCCCAT GACATGTATT 
CTAGGAAAAT GTGCTGTGTT GTCATTCAAG GACTTCCTCT CCTGCAGGCC AACTGAAATT 
CCAGAAAATG ACATTCTGCT TTGTGAGAGC CGCTACAACG AGAGTGACAA GCAGATGAAG 
AAATTTAAGG GACTGAAGAG ATTTTCACTG TCTGCTAAAG TGGTAGATGA TGAAATTTAT 
TACTTCAGAA AACCAATTGT TCCTCAGAAG GAGCCCTCAC CTTTATTGGA AAAGAAGATC 
CAGTTGCTAG AAGCTAAATT TGCTGAGTTA GAAGGTGGAG ATGATGATAT TGAAGAGATG 
GGAGAAGAGG ATAGTGAGGT CATTGAGCCT CCCTCTCTAC CTCAACTTCA GACTCCCCTG 
GCCAGTGAGC TGGATCTCAT GCCCTACACA CCCCCACAGT CTACGCCCAA ATCTGCCAAA 
GGCAGTGCAA AGAAGGAAGG CTCCAAACGG AAGATCAACA TGAGTGGCTA CATCCTGTTC 
AGCAGTGAGA TGAGAGCTGT GATTAAGGCC CAGCACCCAG ACTATTCTTT TGGGGAGCTC 
AGCCGACTGG TGGGGACAGA GTGGAGAAAC CTTGAGACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCCCACCATC TTCCGCCAGG TGTGCCCGGC 
CTCCCGGGCA TCCCACCACC GGGTGTGATG AACCAAGGAG TGGCCCCTAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAG CAGGTAGGTG TTCTGGGGCC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGCCCACAT CCTGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACACCCA TGTTTGTGGC TCCCCCACCG AAGACCCAAC GGCTTCTCCA CTCAGAGGCC 
TACCTAAAAT ACATTGAAGG ACTCAGTGCT GAGTCCAACA GCATTAGCAA GTGGGACCAG 
ACTCTGGCAG CTCGAAGACG TGATGTCCAT CTGTCGAAAG AACAGGAGAG CCGCCTTCCC 
TCTCACTGGC TAAAAAGTAA AGGGGCCCAC ACCACCATGG CAGATGCCCT ATGGCGCCTT 
CGGGATCTGA TGCTCCGAGA CACCCTCAAC ATTCGCCAAG CATACAATCT AGAAAACCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_010817818.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816120.1 Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.

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CloneID	OBa46598
Clone ID Related Accession (Same CDS sequence)	XM_024982486.1 , XM_010817818.2
Accession Version	XM_010817818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCTACCTC CCCTTCCAGT AGTGTCAGTG GGGACTTTGA TGATGGGCAC 
CATTCTGTGT CTACACCAGG CCCAAGCAGG AAAAGGAGGA GACTTTCCAG TCTTCCAACT 
GTAGACCCTA TTGCTGTGTG CCATGAACTC TACAACACCA TCCGAGACTA TAAGGATGAG 
CAGGGCAGGC TCCTCTGTGA GCTCTTCATT AGGGCACCAA AACGAAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTAAAAATG 
GAAGAGTATG ATGATGTTAA TCTGCTTACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTATT ATAAGCCAGA TTCTCCTGAA TATAAAGCTG CTTGCAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAGGGAGAAG CAGATGACGA AGATGATGAT 
GAAGACGGGC AAGACAGTCA AGGCGCAGTG ACGGAAGGAT CTTCTCCAGG TTACTTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGTCTC 
ATAAGCGAAC TTTTTCAGAA GCTGCCTTCT AAAGTGCAAT ATCCAGACTA TTATGCAATT 
ATTAAGGAGC CTATAGATCT CAAGACCATT GCCCAGAGGA TACAGAATGG AAGCTACAAG 
AGTATTCACG CAATGGCCAA AGATATAGAT CTCCTAGCAA AAAATGCCAA AACTTACAAT 
GAGCCTGGTT CTCAAGTGTT CAAGGATGCA AATTCTATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATGA AATGACAAAG TCAAGCCTTC GAATGAGGAC TTCATCAAAT 
TTGGCTGCAA CCAGGCTGAC AGGTCCTTCG CACAGTAAAG GCAGCCTTGG TGAAGAGAGA 
AATCCCACTA GCAAGTATTA CCGTAATAAA AGAGCAGTCC AAGGGGGTCG TTTATCTGCA 
ATTACCATGG CACTTCAGTA TGGCTCAGAA AGTGAAGAGG ATGCTGCTTT AGCTGCTGCG 
CGATATGAAG AAGGAGAATC TGAAGCAGAG AGCATTACTT CATTTATGGA TGTTTCAAAT 
CCTTTTTATC AGCTTTATGA CACAGTTCGG AGCTGTCGGA ATAACCAAGG CCAGCTAATA 
GCTGAACCTT TTTTCCATTT GCCTTCAAAG AAAAAATATC CTGATTATTA TCAGCAAATT 
AAAACACCCA TATCACTACA ACAGATCAGA ACAAAGCTAA AAAACCAAGA ATATGAAACT 
TTAGATCATT TGGAGTGTGA TCTGAATTTA ATGTTTGAAA ATGCCAAACG CTATAATGTT 
CCCAACTCGG CCATCTACAA GCGAGTTCTG AAACTGCAGC AAGTCATGCA GGCAAAGAAG 
AAAGAGCTTG CTAGGAGAGA CGACATTGAG GATGGAGACA GCATGATTTC TTCAGCCACC 
TCTGATACTG GTAGTGCCAA AAGAAAAAGG AGCACTCTTG ACAGTGAGAT GTTGGGTCTC 
AGGAGGCTCT CCAGTAAAAA GAACATAAGA AAGCAGCGAA TGAAAATCTT ATTCAATGTT 
GTTCTCGAAG CTCGTGAGCC AGGCTCAGGC AGAAGACTTT GTGATCTATT TATGGTTAAA 
CCATCCAAAA AGGACTATCC TGATTATTAT AAAATCATCT TGGAGCCAAT GGACTTGAAA 
ATAATTGAGC ATAACATCCG CAATGACAAA TATGCGGGGG AGGAGGGAAT GATAGAGGAC 
ATGAAGCTGA TGTTCCGGAA CGCCAGGCAC TACAACGAGG AGGGCTCCCA GGTGTACAAC 
GATGCACACA TCCTGGAGAA GTTACTCAAG GATAAAAGGA AAGAGCTGGG CCCACTGCCT 
GATGATGATG ACATGGCATC TCCCAAACTC AAGCTGAGTA GGAAGAGTGG TATTTCTCCT 
AAAAAATCAA AATACATGAC TCCAATGCAG CAGAAACTAA ATGAAGTCTA TGAAGCTGTA 
AAGAACTATA CTGATAAGAG GGGTCGCCGC CTCAGTGCCA TATTTCTGAG GCTTCCCTCC 
AGATCCGAGC TGCCTGACTA CTATTTGACC ATTAAAAAGC CCATGGACAT GGAAAAAATT 
CGAAGTCACA TGATGGCCAA CAAATACCAA GATATAGACT CCATGGTTGA GGACTTCGTC 
ATGATGTTTA ACAATGCCTG TACATACAAT GAGCCCGAGT CTTTGATCTA CAAAGATGCC 
CTTGTCCTAC ACAAAGTGCT GCTTGAAACT CGGAGAGACT TAGAAGGAGA TGAGGACTCG 
CATGTCCCAA ATGTAACCTT GCTGATTCAG GAGCTTATCC ATAATCTTTT TGTGTCAGTC 
ATGAGTCATC AGGATGATGA AGGAAGATGC TACAGTGATT CCTTAGCAGA AATTCCTGCT 
GTGGATCCCA GCTTTCCAAA CAAACCTCCT CTTACTTTTG ACATAATTAG GAAGAATGTT 
GAAAATAACC GCTACCGACG GCTTGATTTA TTTCAAGAGC ATATGTTTGA AGTATTGGAG 
AGGGCAAGAA GGATGAATCG GACAGATTCT GAAATATATG AGGATGCGGT AGAACTTCAG 
CAGTTTTTTA TTAAAATTCG TGATGAACTC TGCAAAAATG GAGAGATCCT TCTTTCACCA 
GCACTCAGCT ATACCACAAA ACATTTGCAT AATGATGTGG AGAAAGAAAA GAAGGAAAAA 
CTGCCAAAAG AAATAGAGGA AGATAAACTA AAACGAGAAG AAGAAAAAAG AGAAGCTGAA 
AAGAGTGAAG ATTCCTCAGG AGCTGCAGGC CTCTCAGGCT TACATCGCAC ATACAGCCAG 
GACTGCAGCT TTAAGAACAG CATGTACCAT GTTGGAGATT ACGTCTATGT GGAGCCCTCA 
GAGGTCAATC TACAACCGCA TATCGTCTGT ATTGAGAGAC TGTGGGAAGA TTCTGCTGGT 
GAAAAATGGT TGTATGGCTG TTGGTTTTAT CGGCCAAATG AAACATTCCA TTTGGCTACA 
CGGAAATTTC TAGAAAAAGA AGTTTTTAAG AGTGACTATT ACAATAAAGT TCCTGTTAGT 
AAAATTCTAG GAAAATGTGT GGTCATGTTT GTCAAGGAAT ACTTTAAATT ATGCCCAGAA 
AATTTTCGAG ATGAGGATGT TTTTGTCTGT GAATCACGAT ATTCTGCCAA AACCAAATCT 
TTTAAGAAAA TTAAACTGTG GACCATGCCC GTCAGCTCGG TTAGGTTTGT CCCTCGAGAT 
GTGCCCTTGC CTGTGGTCCG AGTGGCCTCT GTATTTGCAA ACGCAGATAA AGCGGATGAT 
GAGAAGAATA CAGACAACTC AGAGGACAGT CGAACTGAAG ACAGTTGTAA CTTGGAAAAG 
GAGAAAGAAG ATGTCCCTGT GGAAATGTCG AATGGTGAAC CAGGTTGCCA CTACTTTGAG 
CAGCTCCGGT ATAATGACAT GTGGCTGAAG GTTGGCGACT GTGTCTTCAT CAAGTCCCAT 
GGGCTAGTGC GTCCTCGTGT TGGCAGAATT GAAAAGGTAT GGGTCCGAGA TGGAGCTGCG 
TATTTTTATG GCCCCATCTT CATTCACCCA GAAGAGACGG AACATGAGCC GACAAAAATG 
TTCTACAAGA AGGAAGTGTT TTTGAGTAAT CTGGAAGAAA CCTGTCCCAT GACATGTATT 
CTAGGAAAAT GTGCTGTGTT GTCATTCAAG GACTTCCTCT CCTGCAGGCC AACTGAAATT 
CCAGAAAATG ACATTCTGCT TTGTGAGAGC CGCTACAACG AGAGTGACAA GCAGATGAAG 
AAATTTAAGG GACTGAAGAG ATTTTCACTG TCTGCTAAAG TGGTAGATGA TGAAATTTAT 
TACTTCAGAA AACCAATTGT TCCTCAGAAG GAGCCCTCAC CTTTATTGGA AAAGAAGATC 
CAGTTGCTAG AAGCTAAATT TGCTGAGTTA GAAGGTGGAG ATGATGATAT TGAAGAGATG 
GGAGAAGAGG ATAGTGAGGT CATTGAGCCT CCCTCTCTAC CTCAACTTCA GACTCCCCTG 
GCCAGTGAGC TGGATCTCAT GCCCTACACA CCCCCACAGT CTACGCCCAA ATCTGCCAAA 
GGCAGTGCAA AGAAGGAAGG CTCCAAACGG AAGATCAACA TGAGTGGCTA CATCCTGTTC 
AGCAGTGAGA TGAGAGCTGT GATTAAGGCC CAGCACCCAG ACTATTCTTT TGGGGAGCTC 
AGCCGACTGG TGGGGACAGA GTGGAGAAAC CTTGAGACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCCCACCATC TTCCGCCAGG TGTGCCCGGC 
CTCCCGGGCA TCCCACCACC GGGTGTGATG AACCAAGGAG TGGCCCCTAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAG CAGGTAGGTG TTCTGGGGCC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGCCCACAT CCTGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACACCCA TGTTTGTGGC TCCCCCACCG AAGACCCAAC GGCTTCTCCA CTCAGAGGCC 
TACCTAAAAT ACATTGAAGG ACTCAGTGCT GAGTCCAACA GCATTAGCAA GTGGGACCAG 
ACTCTGGCAG CTCGAAGACG TGATGTCCAT CTGTCGAAAG AACAGGAGAG CCGCCTTCCC 
TCTCACTGGC TAAAAAGTAA AGGGGCCCAC ACCACCATGG CAGATGCCCT ATGGCGCCTT 
CGGGATCTGA TGCTCCGAGA CACCCTCAAC ATTCGCCAAG CATACAATCT AGAAAACCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816120.1
CDS	79..5241
Translation	MRRLAFRGAGCALKLDSMGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSSLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDSQGAVTEGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHEMTKSSLRMRTSSNLAATRLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKTPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRSTLDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKDKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPSFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPSEVNLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFANADKADDEKNTDNSEDSRTEDSCNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENL

Target ORF information:

RefSeq Version	XM_010817818.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polybromo 1 (PBRM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817818.2

ORF Insert Sequence:

ATGAGGAGAC TGGCTTTTCG AGGAGCTGGT TGTGCTCTGA AGTTGGATTC CATGGGTTCC 
AAGAGAAGAA GAGCTACCTC CCCTTCCAGT AGTGTCAGTG GGGACTTTGA TGATGGGCAC 
CATTCTGTGT CTACACCAGG CCCAAGCAGG AAAAGGAGGA GACTTTCCAG TCTTCCAACT 
GTAGACCCTA TTGCTGTGTG CCATGAACTC TACAACACCA TCCGAGACTA TAAGGATGAG 
CAGGGCAGGC TCCTCTGTGA GCTCTTCATT AGGGCACCAA AACGAAGAAA TCAACCAGAC 
TATTATGAAG TGGTTTCTCA GCCCATTGAC TTGATGAAAA TCCAACAGAA ACTAAAAATG 
GAAGAGTATG ATGATGTTAA TCTGCTTACT GCTGACTTCC AGCTGCTTTT TAACAATGCA 
AAGGCCTATT ATAAGCCAGA TTCTCCTGAA TATAAAGCTG CTTGCAAACT CTGGGATTTG 
TACCTTCGAA CAAGAAATGA GTTTGTTCAG AAGGGAGAAG CAGATGACGA AGATGATGAT 
GAAGACGGGC AAGACAGTCA AGGCGCAGTG ACGGAAGGAT CTTCTCCAGG TTACTTGAAG 
GAGATCCTGG AGCAGCTTCT TGAAGCCATA GTTGTAGCCA CAAATCCATC AGGACGTCTC 
ATAAGCGAAC TTTTTCAGAA GCTGCCTTCT AAAGTGCAAT ATCCAGACTA TTATGCAATT 
ATTAAGGAGC CTATAGATCT CAAGACCATT GCCCAGAGGA TACAGAATGG AAGCTACAAG 
AGTATTCACG CAATGGCCAA AGATATAGAT CTCCTAGCAA AAAATGCCAA AACTTACAAT 
GAGCCTGGTT CTCAAGTGTT CAAGGATGCA AATTCTATTA AAAAAATATT TTATATGAAA 
AAGGCAGAAA TTGAACATGA AATGACAAAG TCAAGCCTTC GAATGAGGAC TTCATCAAAT 
TTGGCTGCAA CCAGGCTGAC AGGTCCTTCG CACAGTAAAG GCAGCCTTGG TGAAGAGAGA 
AATCCCACTA GCAAGTATTA CCGTAATAAA AGAGCAGTCC AAGGGGGTCG TTTATCTGCA 
ATTACCATGG CACTTCAGTA TGGCTCAGAA AGTGAAGAGG ATGCTGCTTT AGCTGCTGCG 
CGATATGAAG AAGGAGAATC TGAAGCAGAG AGCATTACTT CATTTATGGA TGTTTCAAAT 
CCTTTTTATC AGCTTTATGA CACAGTTCGG AGCTGTCGGA ATAACCAAGG CCAGCTAATA 
GCTGAACCTT TTTTCCATTT GCCTTCAAAG AAAAAATATC CTGATTATTA TCAGCAAATT 
AAAACACCCA TATCACTACA ACAGATCAGA ACAAAGCTAA AAAACCAAGA ATATGAAACT 
TTAGATCATT TGGAGTGTGA TCTGAATTTA ATGTTTGAAA ATGCCAAACG CTATAATGTT 
CCCAACTCGG CCATCTACAA GCGAGTTCTG AAACTGCAGC AAGTCATGCA GGCAAAGAAG 
AAAGAGCTTG CTAGGAGAGA CGACATTGAG GATGGAGACA GCATGATTTC TTCAGCCACC 
TCTGATACTG GTAGTGCCAA AAGAAAAAGG AGCACTCTTG ACAGTGAGAT GTTGGGTCTC 
AGGAGGCTCT CCAGTAAAAA GAACATAAGA AAGCAGCGAA TGAAAATCTT ATTCAATGTT 
GTTCTCGAAG CTCGTGAGCC AGGCTCAGGC AGAAGACTTT GTGATCTATT TATGGTTAAA 
CCATCCAAAA AGGACTATCC TGATTATTAT AAAATCATCT TGGAGCCAAT GGACTTGAAA 
ATAATTGAGC ATAACATCCG CAATGACAAA TATGCGGGGG AGGAGGGAAT GATAGAGGAC 
ATGAAGCTGA TGTTCCGGAA CGCCAGGCAC TACAACGAGG AGGGCTCCCA GGTGTACAAC 
GATGCACACA TCCTGGAGAA GTTACTCAAG GATAAAAGGA AAGAGCTGGG CCCACTGCCT 
GATGATGATG ACATGGCATC TCCCAAACTC AAGCTGAGTA GGAAGAGTGG TATTTCTCCT 
AAAAAATCAA AATACATGAC TCCAATGCAG CAGAAACTAA ATGAAGTCTA TGAAGCTGTA 
AAGAACTATA CTGATAAGAG GGGTCGCCGC CTCAGTGCCA TATTTCTGAG GCTTCCCTCC 
AGATCCGAGC TGCCTGACTA CTATTTGACC ATTAAAAAGC CCATGGACAT GGAAAAAATT 
CGAAGTCACA TGATGGCCAA CAAATACCAA GATATAGACT CCATGGTTGA GGACTTCGTC 
ATGATGTTTA ACAATGCCTG TACATACAAT GAGCCCGAGT CTTTGATCTA CAAAGATGCC 
CTTGTCCTAC ACAAAGTGCT GCTTGAAACT CGGAGAGACT TAGAAGGAGA TGAGGACTCG 
CATGTCCCAA ATGTAACCTT GCTGATTCAG GAGCTTATCC ATAATCTTTT TGTGTCAGTC 
ATGAGTCATC AGGATGATGA AGGAAGATGC TACAGTGATT CCTTAGCAGA AATTCCTGCT 
GTGGATCCCA GCTTTCCAAA CAAACCTCCT CTTACTTTTG ACATAATTAG GAAGAATGTT 
GAAAATAACC GCTACCGACG GCTTGATTTA TTTCAAGAGC ATATGTTTGA AGTATTGGAG 
AGGGCAAGAA GGATGAATCG GACAGATTCT GAAATATATG AGGATGCGGT AGAACTTCAG 
CAGTTTTTTA TTAAAATTCG TGATGAACTC TGCAAAAATG GAGAGATCCT TCTTTCACCA 
GCACTCAGCT ATACCACAAA ACATTTGCAT AATGATGTGG AGAAAGAAAA GAAGGAAAAA 
CTGCCAAAAG AAATAGAGGA AGATAAACTA AAACGAGAAG AAGAAAAAAG AGAAGCTGAA 
AAGAGTGAAG ATTCCTCAGG AGCTGCAGGC CTCTCAGGCT TACATCGCAC ATACAGCCAG 
GACTGCAGCT TTAAGAACAG CATGTACCAT GTTGGAGATT ACGTCTATGT GGAGCCCTCA 
GAGGTCAATC TACAACCGCA TATCGTCTGT ATTGAGAGAC TGTGGGAAGA TTCTGCTGGT 
GAAAAATGGT TGTATGGCTG TTGGTTTTAT CGGCCAAATG AAACATTCCA TTTGGCTACA 
CGGAAATTTC TAGAAAAAGA AGTTTTTAAG AGTGACTATT ACAATAAAGT TCCTGTTAGT 
AAAATTCTAG GAAAATGTGT GGTCATGTTT GTCAAGGAAT ACTTTAAATT ATGCCCAGAA 
AATTTTCGAG ATGAGGATGT TTTTGTCTGT GAATCACGAT ATTCTGCCAA AACCAAATCT 
TTTAAGAAAA TTAAACTGTG GACCATGCCC GTCAGCTCGG TTAGGTTTGT CCCTCGAGAT 
GTGCCCTTGC CTGTGGTCCG AGTGGCCTCT GTATTTGCAA ACGCAGATAA AGCGGATGAT 
GAGAAGAATA CAGACAACTC AGAGGACAGT CGAACTGAAG ACAGTTGTAA CTTGGAAAAG 
GAGAAAGAAG ATGTCCCTGT GGAAATGTCG AATGGTGAAC CAGGTTGCCA CTACTTTGAG 
CAGCTCCGGT ATAATGACAT GTGGCTGAAG GTTGGCGACT GTGTCTTCAT CAAGTCCCAT 
GGGCTAGTGC GTCCTCGTGT TGGCAGAATT GAAAAGGTAT GGGTCCGAGA TGGAGCTGCG 
TATTTTTATG GCCCCATCTT CATTCACCCA GAAGAGACGG AACATGAGCC GACAAAAATG 
TTCTACAAGA AGGAAGTGTT TTTGAGTAAT CTGGAAGAAA CCTGTCCCAT GACATGTATT 
CTAGGAAAAT GTGCTGTGTT GTCATTCAAG GACTTCCTCT CCTGCAGGCC AACTGAAATT 
CCAGAAAATG ACATTCTGCT TTGTGAGAGC CGCTACAACG AGAGTGACAA GCAGATGAAG 
AAATTTAAGG GACTGAAGAG ATTTTCACTG TCTGCTAAAG TGGTAGATGA TGAAATTTAT 
TACTTCAGAA AACCAATTGT TCCTCAGAAG GAGCCCTCAC CTTTATTGGA AAAGAAGATC 
CAGTTGCTAG AAGCTAAATT TGCTGAGTTA GAAGGTGGAG ATGATGATAT TGAAGAGATG 
GGAGAAGAGG ATAGTGAGGT CATTGAGCCT CCCTCTCTAC CTCAACTTCA GACTCCCCTG 
GCCAGTGAGC TGGATCTCAT GCCCTACACA CCCCCACAGT CTACGCCCAA ATCTGCCAAA 
GGCAGTGCAA AGAAGGAAGG CTCCAAACGG AAGATCAACA TGAGTGGCTA CATCCTGTTC 
AGCAGTGAGA TGAGAGCTGT GATTAAGGCC CAGCACCCAG ACTATTCTTT TGGGGAGCTC 
AGCCGACTGG TGGGGACAGA GTGGAGAAAC CTTGAGACAG CCAAGAAAGC AGAATATGAA 
GAGCGGGCAG CTAAAGTTGC TGAGCAGCAG GAGAGAGAGC GAGCAGCACA GCAACAGCAG 
CCGAGTGCTT CTCCCCGAGC AGGCACCCCT GTGGGGGCTC TCATGGGGGT GGTGCCACCA 
CCAACACCAA TGGGGATGCT CAATCAGCAG TTGACACCTG TTGCAGGCAT GATGGGTGGC 
TATCCGCCAG GCCTTCCACC TTTGCAGGGC CCAGTTGATG GCCTTGTTAG CATGGGCAGC 
ATGCAGCCAC TTCACCCTGG GGGGCCTCCA CCCCACCATC TTCCGCCAGG TGTGCCCGGC 
CTCCCGGGCA TCCCACCACC GGGTGTGATG AACCAAGGAG TGGCCCCTAT GGTAGGGACT 
CCAGCACCAG GTGGAAGTCC GTATGGACAG CAGGTAGGTG TTCTGGGGCC TCCAGGGCAG 
CAGGCACCAC CTCCATATCC TGGCCCACAT CCTGCTGGCC CCCCTGTCAT ACAGCAGCCA 
ACAACACCCA TGTTTGTGGC TCCCCCACCG AAGACCCAAC GGCTTCTCCA CTCAGAGGCC 
TACCTAAAAT ACATTGAAGG ACTCAGTGCT GAGTCCAACA GCATTAGCAA GTGGGACCAG 
ACTCTGGCAG CTCGAAGACG TGATGTCCAT CTGTCGAAAG AACAGGAGAG CCGCCTTCCC 
TCTCACTGGC TAAAAAGTAA AGGGGCCCAC ACCACCATGG CAGATGCCCT ATGGCGCCTT 
CGGGATCTGA TGCTCCGAGA CACCCTCAAC ATTCGCCAAG CATACAATCT AGAAAACCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PBRM1
Entrez Gene ID	506557
Full Name	polybromo 1
Synonyms	PB1
General protein information	Preferred Names polybromo 1 Names protein polybromo-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Drosophila melanogaster (fruit fly)	polybromo	NP_651288.1
Xenopus tropicalis (tropical clawed frog)	pbrm1	XP_004919938.1
Homo sapiens (human)	PBRM1	NP_060783.3
	PBRM1	XP_001088172.2
Rattus norvegicus (Norway rat)	Pbrm1	XP_006252749.1
Canis lupus familiaris (dog)	PBRM1	XP_005632499.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001099	XP_322068.4
Pan troglodytes (chimpanzee)	PBRM1	XP_516515.3
Mus musculus (house mouse)	Pbrm1	NP_001074720.1
Gallus gallus (chicken)	PBRM1	NP_990496.1
Bos taurus (cattle)	PBRM1	XP_005223037.1
Danio rerio (zebrafish)	pbrm1	XP_693735.5
Caenorhabditis elegans (roundworm)	pbrm-1	NP_001021008.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PBRM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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